CN102487968B - Plant source sterilization preparation and application thereof in prevention and treatment of rice sheath blight - Google Patents
Plant source sterilization preparation and application thereof in prevention and treatment of rice sheath blight Download PDFInfo
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- 235000009566 rice Nutrition 0.000 title claims abstract description 38
- 238000002360 preparation method Methods 0.000 title claims abstract description 33
- 241000196324 Embryophyta Species 0.000 title claims abstract description 19
- 240000007594 Oryza sativa Species 0.000 title claims 2
- 238000011282 treatment Methods 0.000 title description 16
- 230000002265 prevention Effects 0.000 title description 3
- 230000001954 sterilising effect Effects 0.000 title 1
- 238000004659 sterilization and disinfection Methods 0.000 title 1
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 44
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- AXTGDCSMTYGJND-UHFFFAOYSA-N 1-dodecylazepan-2-one Chemical compound CCCCCCCCCCCCN1CCCCCC1=O AXTGDCSMTYGJND-UHFFFAOYSA-N 0.000 claims abstract description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 17
- 230000000844 anti-bacterial effect Effects 0.000 claims abstract description 14
- 239000000417 fungicide Substances 0.000 claims abstract description 11
- 230000000855 fungicidal effect Effects 0.000 claims abstract description 10
- 230000001737 promoting effect Effects 0.000 claims abstract description 4
- 229920000056 polyoxyethylene ether Polymers 0.000 claims description 15
- 239000000203 mixture Substances 0.000 claims description 12
- 229940051841 polyoxyethylene ether Drugs 0.000 claims description 12
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 claims description 9
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 5
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- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 4
- BDHFUVZGWQCTTF-UHFFFAOYSA-M sulfonate Chemical compound [O-]S(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-M 0.000 claims description 4
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical class C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 claims description 3
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- 150000002191 fatty alcohols Chemical class 0.000 claims description 3
- ZEMPKEQAKRGZGQ-XOQCFJPHSA-N glycerol triricinoleate Natural products CCCCCC[C@@H](O)CC=CCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCC=CC[C@@H](O)CCCCCC)OC(=O)CCCCCCCC=CC[C@H](O)CCCCCC ZEMPKEQAKRGZGQ-XOQCFJPHSA-N 0.000 claims description 3
- 239000011347 resin Substances 0.000 claims description 3
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- DMAXMXPDVWTIRV-UHFFFAOYSA-N 2-(2-phenylethyl)phenol Chemical group OC1=CC=CC=C1CCC1=CC=CC=C1 DMAXMXPDVWTIRV-UHFFFAOYSA-N 0.000 claims description 2
- 229920002503 polyoxyethylene-polyoxypropylene Polymers 0.000 claims description 2
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- BVTJGGGYKAMDBN-UHFFFAOYSA-N Dioxetane Chemical compound C1COO1 BVTJGGGYKAMDBN-UHFFFAOYSA-N 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
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- 240000003768 Solanum lycopersicum Species 0.000 description 1
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- STJLVHWMYQXCPB-UHFFFAOYSA-N propiconazole Chemical compound O1C(CCC)COC1(C=1C(=CC(Cl)=CC=1)Cl)CN1N=CN=C1 STJLVHWMYQXCPB-UHFFFAOYSA-N 0.000 description 1
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
本发明公开了一种植物源杀菌制剂,它包含如下重量百分数的组分:麝香草酚0.1~50%,乳化剂5~30%,氮酮0.1~20%,乙醇10~60%,水0.1~84.8%。本发明还公开了上述植物源杀菌制剂的制备方法,及其在防治水稻纹枯病中的应用。本发明的基于麝香草酚的植物源杀菌制剂其药效成分来源于具有杀虫功能的植物或其提取物,不仅能有效防治水稻纹枯病,同时具有促进作物生长发育、增产增收、改善品质、提高养分利用率、防治害虫、无污染等多重功效,适用于无公害、绿色和有机农产品生产。该制剂应用效果显著、原材料易得,生产工艺简单、市场潜力大、经济效益和社会效益明显,具有十分广阔的应用前景。The invention discloses a plant source fungicidal preparation, which comprises the following components in weight percent: 0.1-50% thymol, 5-30% emulsifier, 0.1-20% azone, 10-60% ethanol, and 0.1% water ~84.8%. The invention also discloses a preparation method of the above-mentioned botanical bactericidal preparation and its application in preventing and treating rice sheath blight. The thymol-based botanical fungicide preparation of the present invention has medicinal ingredients derived from plants with insecticidal functions or their extracts, which can not only effectively prevent and control rice sheath blight, but also have the functions of promoting crop growth and development, increasing production and income, and improving quality. , Improve nutrient utilization efficiency, prevent pests, and have multiple functions such as no pollution. It is suitable for pollution-free, green and organic agricultural production. The preparation has obvious application effects, easy-to-obtain raw materials, simple production process, great market potential, obvious economic and social benefits, and has very broad application prospects.
Description
技术领域 technical field
本发明属于农业生产中作物防病治病技术领域,具体涉及一种植物源杀菌制剂及其在防治水稻纹枯病中的应用。The invention belongs to the technical field of crop disease prevention and treatment in agricultural production, and in particular relates to a botanical bactericidal preparation and its application in preventing and treating rice sheath blight.
背景技术 Background technique
水稻纹枯病俗名“花脚杆”、“烂脚瘟”、“云纹病”,属热带系病害,是水稻常发性和重发性病害之一。20世纪90年代中期对我国南方稻区的调查结果表明:水稻纹枯病的发病面积是稻瘟病的2.07倍,造成的损失是稻瘟病的1.97倍,其发生危害程度已远远超过水稻稻瘟病、条纹叶枯病等,成为水稻第一大病害,给水稻高产稳产带来严重威胁。近年来受气候异常、土壤菌量累积量增高,防治措施不及时与农田粗放的管理等原因,水稻纹枯病发生危害普遍较为严重。发病率高达60%左右,受害较重的田块,造成植株不能正常抽穗,结实率降低,籽粒不饱满,或严重倒伏,严重影响水稻产量。水稻纹枯病在我国各稻区均有分布,在长江以南稻区发生普遍,危害性也大。在东北地区,辽宁省发生较重,已成为当地水稻的主要病害之一。黑龙江省近几年来也有所发生,稻株受害后瘪谷率增加,千粒重降低如果引起倒伏,茎叶腐烂,则损失更大。Rice sheath blight, commonly known as "flower foot stem", "rotten foot blast", and "moire disease", belongs to tropical diseases and is one of the frequent and recurrent diseases of rice. In the middle of the 1990s, the survey results of the southern rice areas in my country showed that the incidence area of rice sheath blight was 2.07 times that of rice blast, and the loss caused was 1.97 times that of rice blast, and its damage degree has far exceeded that of rice blast. , stripe leaf blight, etc., have become the number one disease of rice, posing a serious threat to high and stable rice yields. In recent years, rice sheath blight is generally more serious due to abnormal climate, increased accumulation of soil bacteria, untimely control measures and extensive management of farmland. The incidence rate is as high as about 60%. In the heavily damaged fields, the plants cannot be heading normally, the seed setting rate is reduced, the grains are not full, or they are seriously lodging, which seriously affects the rice yield. Rice sheath blight is distributed in all rice areas in my country, and it is common and harmful in the rice areas south of the Yangtze River. In Northeast China, Liaoning Province has a severe occurrence and has become one of the main diseases of local rice. Heilongjiang Province has also happened in recent years. After rice plants are damaged, the shriveling rate increases. If the thousand-grain weight decreases, if it causes lodging and stem and leaf rot, the loss will be even greater.
近几十年来,人们已在水稻纹枯病的防治上进行了一系列化学防治技术研究,取得了一定的结果。目前对水稻纹枯病的药剂防治主要还是以化学农药井冈霉素为主。井冈霉素是防治水稻纹枯病的主要药种,该药具有内吸传导性,兼有预防和治疗作用,但其持效期较短,在近年来水稻纹枯病发生严重的情况下,井冈霉素的防效更是难以保证。同时随着化学农药的逐年使用纹枯病的抗药性也在逐渐增加,为此农民加大农药的使用量,若过度加大用量,不仅会增加作物中农药残留量,抑制土壤中有益微生物生长,而且会污染环境,增加生产成本。由此,农药使用和依赖程度呈现出恶性循环状态。In recent decades, people have carried out a series of chemical control technology research on the control of rice sheath blight, and achieved certain results. At present, the chemical pesticide control of rice sheath blight is mainly based on the chemical pesticide Jinggangmycin. Jinggangmycin is the main drug for the control of rice sheath blight. The drug has systemic conductivity and has both preventive and therapeutic effects, but its duration is short. In recent years, when rice sheath blight has occurred seriously, The control effect of Jinggangmycin is even more difficult to guarantee. At the same time, with the use of chemical pesticides year by year, the resistance of sheath blight is gradually increasing. For this reason, farmers increase the use of pesticides. If the amount is excessively increased, it will not only increase the amount of pesticide residues in crops, but also inhibit the growth of beneficial microorganisms in the soil. , and will pollute the environment and increase production costs. As a result, the use and dependence of pesticides presents a vicious cycle.
随着食品安全事件的频繁发生,国家对食品安全的重视,开发研制出高效、环境友好型植物源生物农药来替代高毒、高残留的化学农药就成为农业科学研究的一个热点。通过植物源天然化合物改良后制成的生物农药,一方面对植株的果、花、叶都无危害,对人畜安全,具有高效无残留,无药害,无抗性药性等优点;另一方面在控制病害的同时可以有效提高农产品品质。With the frequent occurrence of food safety incidents and the country's emphasis on food safety, it has become a hot spot in agricultural scientific research to develop efficient and environmentally friendly plant-derived biological pesticides to replace highly toxic and high-residue chemical pesticides. Biopesticides made by improving natural compounds of plant origin, on the one hand, have no harm to the fruits, flowers, and leaves of plants, are safe to humans and animals, and have the advantages of high efficiency, no residue, no harm, and no drug resistance; on the other hand, It can effectively improve the quality of agricultural products while controlling diseases.
发明内容Contents of the invention
本发明所要解决的技术问题是提供一种基于麝香草酚的植物源杀菌制剂,该制剂是一种集抗菌、营养、改善品质于一体的多功效新型环境友好制剂。The technical problem to be solved by the present invention is to provide a thymol-based botanical bactericidal preparation, which is a new multifunctional environment-friendly preparation integrating antibacterial, nutrition and quality improvement.
本发明还要解决的技术问题是提供上述基于麝香草酚的植物源杀菌制剂的制备方法。The technical problem to be solved in the present invention is to provide a preparation method of the above-mentioned thymol-based plant source fungicide.
本发明最后要解决的技术问题是提供上述植物源杀菌制剂在防治水稻纹枯病中的应用。The final technical problem to be solved by the present invention is to provide the application of the above-mentioned botanical fungicides in the prevention and treatment of rice sheath blight.
为解决上述技术问题,本发明采用的技术方案如下:In order to solve the problems of the technologies described above, the technical scheme adopted in the present invention is as follows:
一种植物源杀菌制剂,它包含如下重量百分数的组分:麝香草酚0.1~50%,乳化剂5~30%,氮酮0.1~20%,乙醇10~60%,水0.1~84.8%。A plant source fungicide preparation, which comprises the following components in weight percent: 0.1-50% thymol, 5-30% emulsifier, 0.1-20% azone, 10-60% ethanol, and 0.1-84.8% water.
上述植物源杀菌制剂,优选包含如下重量百分数的组分:麝香草酚0.5~5%,乳化剂10~20%,氮酮1~2%,乙醇20~40%,水33~68%。The plant-derived bactericidal preparation preferably contains the following components in weight percent: 0.5-5% thymol, 10-20% emulsifier, 1-2% azone, 20-40% ethanol, and 33-68% water.
其中,所述的乳化剂为苯乙基酚聚氧乙烯醚、苯乙基苯酚聚氧乙烯聚氧丙烯醚、芳烷基酚聚氧乙烯醚磷酸酯、烷基酚聚氧乙烯醚磷酸酯、烷基萘磺酸钙、蓖麻油环氧乙烷加成物、芳烷基酚聚氧乙烯醚、脂肪醇聚氧乙烯醚、烷基酚甲醛树脂聚氧乙烯醚和二烷基苯磺酸钙中的一种或几种的混合物。Wherein, the emulsifier is styrene-ethylphenol polyoxyethylene ether, styrene-ethylphenol polyoxyethylene polyoxypropylene ether, aralkylphenol polyoxyethylene ether phosphate, alkylphenol polyoxyethylene ether phosphate, Calcium alkylnaphthalene sulfonate, castor oil ethylene oxide adduct, aralkylphenol ethoxylates, fatty alcohol ethoxylates, alkylphenol formaldehyde resin ethoxylates and calcium dialkylbenzene sulfonate one or a mixture of several.
上述麝香草酚可与井冈霉素和丙环唑进行复配。The above thymol can be compounded with Jinggangmycin and propiconazole.
上述植物源杀菌制剂的制备方法,按配方量准备原料麝香草酚、乳化剂、氮酮、乙醇和水,将麝香草酚加入部分乙醇中混合均匀形成溶液A,再将乳化剂和氮酮用剩余乙醇溶解形成溶液B,然后将溶液A、溶液B和水混合均匀即得。The preparation method of the above-mentioned botanical bactericidal preparation, prepare raw materials thymol, emulsifier, azone, ethanol and water according to the formula quantity, add thymol to part of ethanol and mix uniformly to form solution A, then use emulsifier and azone Dissolve the remaining ethanol to form solution B, and then mix solution A, solution B and water evenly.
其中,乙醇分两次使用(即上述部分乙醇和上述剩余乙醇),每次的用量需保证所溶解原料的充分溶解,且两次的用量之和为配方量的乙醇原料总量。Wherein, ethanol is used twice (being above-mentioned part ethanol and above-mentioned remaining ethanol), and each consumption needs to guarantee the fully dissolving of dissolved raw material, and the sum of two consumptions is the ethanol raw material total amount of formula quantity.
上述植物源杀菌制剂在促进水稻生长中的应用。Application of the above-mentioned botanical bactericidal preparation in promoting the growth of rice.
上述植物源杀菌制剂在防治水稻纹枯病中的应用。The application of the above-mentioned botanical fungicide preparation in the prevention and treatment of rice sheath blight.
有益效果:本发明的基于麝香草酚的植物源杀菌制剂其药效成分来源于具有杀虫功能的植物或其提取物,不仅能有效防治水稻纹枯病,同时具有促进作物生长发育、增产增收、改善品质、提高养分利用率、防治害虫、无污染等多重功效,适用于无公害、绿色和有机农产品生产。该制剂应用效果显著、原材料易得,生产工艺简单、市场潜力大、经济效益和社会效益明显,具有十分广阔的应用前景。Beneficial effects: The medicinal ingredients of the thymol-based botanical fungicidal preparation of the present invention are derived from plants with insecticidal functions or their extracts, which can not only effectively prevent and control rice sheath blight, but also have the functions of promoting crop growth and development, increasing production and income , Improve quality, increase nutrient utilization efficiency, control pests, and have multiple functions such as pollution-free, suitable for pollution-free, green and organic agricultural production. The preparation has obvious application effects, easy-to-obtain raw materials, simple production process, great market potential, obvious economic and social benefits, and has very broad application prospects.
具体实施方式Detailed ways
根据下述实施例,可以更好地理解本发明。然而,本领域的技术人员容易理解,实施例所描述的仅用于说明本发明,而不应当也不会限制权利要求书中所详细描述的本发明。The present invention can be better understood from the following examples. However, those skilled in the art can easily understand that the description of the embodiments is only for illustrating the present invention, and should not and will not limit the present invention described in the claims.
实施例1:Example 1:
一种植物源杀菌制剂,它包含如下重量百分数的组分:麝香草酚1%,乳化剂(乳化剂为苯乙基酚聚氧乙烯醚)15%,氮酮2%,乙醇30%,水52%。A kind of plant source bactericidal preparation, it comprises the component of following percentage by weight: Thymol 1%, emulsifier (emulsifier is phenethylphenol polyoxyethylene ether) 15%, azone 2%, ethanol 30%, water 52%.
上述植物源杀菌制剂的制备方法,按配方量准备原料麝香草酚、乳化剂、氮酮、乙醇和水,将麝香草酚加入1/4量的乙醇中混合均匀形成溶液A,再将乳化剂和氮酮用剩余3/4量的乙醇溶解形成溶液B,然后将溶液A、溶液B和水混合均匀即得。The preparation method of the above-mentioned plant-derived bactericidal preparation, prepare raw materials thymol, emulsifier, azone, ethanol and water according to the formula amount, add thymol to 1/4 amount of ethanol and mix uniformly to form solution A, and then emulsifier Dissolve azone and azone with the remaining 3/4 amount of ethanol to form solution B, and then mix solution A, solution B and water evenly.
实施例2:Example 2:
一种植物源杀菌制剂,它包含如下重量百分数的组分:麝香草酚2%,乳化剂(乳化剂为乳化剂为芳烷基酚聚氧乙烯醚磷酸酯和烷基酚聚氧乙烯醚磷酸酯按重量比1∶1的混合物)20%,氮酮2%,乙醇40%,水36%。A kind of plant source bactericidal preparation, it comprises the component of following percentage by weight: Thymol 2%, emulsifying agent (emulsifying agent is that emulsifying agent is aralkylphenol polyoxyethylene ether phosphoric acid ester and alkylphenol polyoxyethylene ether phosphoric acid ester Ester (1:1 mixture by weight) 20%, azone 2%, ethanol 40%, water 36%.
上述植物源杀菌制剂的制备方法,按配方量准备原料麝香草酚、乳化剂、氮酮、乙醇和水,将麝香草酚加入1/2量的乙醇中混合均匀形成溶液A,再将乳化剂和氮酮用剩余1/2量的乙醇溶解形成溶液B,然后将溶液A、溶液B和水混合均匀即得。The preparation method of the above-mentioned plant-derived bactericidal preparation, prepare raw materials thymol, emulsifier, azone, ethanol and water according to the formula quantity, add thymol to 1/2 amount of ethanol and mix uniformly to form solution A, and then emulsifier Dissolve azone and azone with the remaining 1/2 amount of ethanol to form solution B, and then mix solution A, solution B and water evenly.
实施例3:Example 3:
一种植物源杀菌制剂,它包含如下重量百分数的组分:麝香草酚0.5%,乳化剂(乳化剂为烷基酚聚氧乙烯醚磷酸酯)10%,氮酮2%,乙醇30%,水57.5%。A plant source fungicide preparation, which comprises the following components in percentage by weight: thymol 0.5%, emulsifier (emulsifier is alkylphenol polyoxyethylene ether phosphate) 10%, azone 2%, ethanol 30%, Water 57.5%.
制备方法同实施例2。The preparation method is the same as in Example 2.
实施例4:Example 4:
一种植物源杀菌制剂,它包含如下重量百分数的组分:麝香草酚0.1%,乳化剂(乳化剂为烷基萘磺酸钙与蓖麻油环氧乙烷加成物按重量比1∶2的混合物)30%,氮酮0.1%,乙醇60%,水9.8%。A kind of plant source bactericidal preparation, it comprises the component of following percentage by weight: Thymol 0.1%, emulsifying agent (emulsifying agent is calcium alkylnaphthalene sulfonate and castor oil ethylene oxide adduct by weight ratio 1: 2 mixture) 30%, azone 0.1%, ethanol 60%, water 9.8%.
制备方法同实施例2。The preparation method is the same as in Example 2.
实施例5:Example 5:
一种植物源杀菌制剂,它包含如下重量百分数的组分:麝香草酚50%,乳化剂(乳化剂为芳烷基酚聚氧乙烯醚、脂肪醇聚氧乙烯醚和烷基酚甲醛树脂聚氧乙烯醚按重量比1∶2∶1的混合物)5%,氮酮20%,乙醇10%,水15%。A kind of plant source bactericidal preparation, it comprises the component of following percentage by weight: Thymol 50%, emulsifying agent (emulsifying agent is aralkylphenol polyoxyethylene ether, fatty alcohol polyoxyethylene ether and alkylphenol formaldehyde resin polyoxyethylene ether) Oxyethylene ether (1:2:1 mixture by weight) 5%, azone 20%, ethanol 10%, water 15%.
制备方法同实施例2。The preparation method is the same as in Example 2.
实施例6:基于麝香草酚的植物源杀菌制剂对作物生长的促进和对品质的改善作用。Example 6: Thymol-based botanical fungicides promote crop growth and improve quality.
配方I:1/2Hoagland营养液。Recipe I: 1/2 Hoagland nutrient solution.
配方II:实施例1制得的麝香草酚的植物源杀菌制剂。Formula II: the botanical fungicide preparation of thymol prepared in Example 1.
选取一个田块设计三个处理:处理1:对照(对照CK为清水);处理2:配方I;处理3:配方II。每个处理重复3次,处理液每隔10d喷施一次,连喷施5次后进行分析。Select a field to design three treatments: treatment 1: control (control CK is clear water); treatment 2: formula I; treatment 3: formula II. Each treatment was repeated 3 times, and the treatment solution was sprayed every 10 days, and the analysis was carried out after 5 consecutive sprays.
表1不同处理对水稻相关产量指标的影响Table 1 Effects of different treatments on rice related yield indicators
由表1可知,在配方I处理后水稻穗数、单穗重、千粒重、结实率和产量分别比对照增加了4.7%、9.9%、10.4%、8.1%和12.5%;配方II处理后水稻穗数、单穗重、千粒重、结实率和产量分别比对照增加了13.4%、32.3%、23.1%、12.8%和23.8%。It can be seen from Table 1 that the number of panicles, single panicle weight, thousand-grain weight, seed setting rate and yield of rice increased by 4.7%, 9.9%, 10.4%, 8.1% and 12.5% respectively after formula I treatment; The number, weight per ear, thousand-grain weight, seed setting rate and yield increased by 13.4%, 32.3%, 23.1%, 12.8% and 23.8% respectively compared with the control.
有上可知,以配方II处理对水稻生长效果最好。It can be known from the above that the treatment with formula II has the best effect on rice growth.
实施例7:基于麝香草酚的植物源杀菌制剂在对作物水稻纹枯病的防治作用。Example 7: Control effect of thymol-based botanical fungicides on crop rice sheath blight.
配方I:5%井冈霉素水剂。Formula I: 5% Jinggangmycin aqueous solution.
配方II:实施例1制得的麝香草酚的植物源杀菌制剂。Formula II: the botanical fungicide preparation of thymol prepared in Example 1.
选取发病田块设计四个处理:处理1:对照(对照CK为清水);处理2:配方I;处理Choose diseased field piece design four and handle: handle 1: contrast (contrast CK is clear water); Process 2: formula I;
3:配方II。每个处理重复3次,处理液每隔7d喷施一次,连喷施3次后进行分析。3: Formulation II. Each treatment was repeated 3 times, and the treatment solution was sprayed every 7 days, and the analysis was carried out after 3 consecutive sprays.
测定方法:test methods:
病情指数和防治效果的测定:参照窦瑞木方法。(窦瑞木.3株植物内生细菌对番茄灰霉病的防治效果.河南农业科学,2010,4:77-79.)Determination of disease index and control effect: refer to Dou Ruimu's method. (Dou Ruimu. Control effect of three endophytic bacteria on tomato gray mold. Henan Agricultural Sciences, 2010, 4: 77-79.)
由表2可知,在水稻上,配方I对水稻纹枯病的防效为48.38%;配方II对水稻纹枯病的防效为77.22%。It can be known from Table 2 that on rice, the control effect of formula I on rice sheath blight is 48.38%; the control effect of formula II on rice sheath blight is 77.22%.
由上可知,以配方II处理对水稻上水稻纹枯病的防治效果最好。It can be seen from the above that the control effect of rice sheath blight on rice is best treated with formula II.
表2不同处理对水稻纹枯病防治效果Table 2 Control effect of different treatments on rice sheath blight
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