CN102388869B - Botanical fungicide and its application in control of strawberry botrytis cinerea - Google Patents
Botanical fungicide and its application in control of strawberry botrytis cinerea Download PDFInfo
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- CN102388869B CN102388869B CN 201110400440 CN201110400440A CN102388869B CN 102388869 B CN102388869 B CN 102388869B CN 201110400440 CN201110400440 CN 201110400440 CN 201110400440 A CN201110400440 A CN 201110400440A CN 102388869 B CN102388869 B CN 102388869B
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a botanical fungicide which comprises the following components by weight: 0.1-50% of thymol, 5-30% of emulsifiers, 0.1-20% of azone, 10-60% of ethanol, 0.1-84.8% of water. The invention also discloses a preparation method of the botanical fungicide, and an application in the control of strawberry botrytis cinerea. The medical components of the thymol-based botanical fungicide of the invention are derived from plants or extracts which have an insecticidal function, and the fungicide not only can effectively control crop botrytis cinerea, but also has multiple efficacy of promoting crop growth and development, increasing production and income, improving quality, increasing nutrient utilization rate, controlling insects, causing no pollution, and the like, and is applicable to the production of pollution-free, green, and organic agricultural products. The preparation has significant application effect, easily available raw materials, simple production process, large market potential, obvious economic benefit and social benefit, and has extremely extensive application prospects.
Description
Technical field
The invention belongs in the agricultural production fruits and vegetables technical field of preventing and curing diseases, be specifically related to a kind of plant resource biocide preparation and the application in the control grey mould fruit rot of strawberry thereof.
Background technology
Grey mould fruit rot of strawberry is rot again, is one of important disease during current strawberry produces.In recent years, along with the expansion of cultivated strawberry area, spreading gradually of grey mould fruit rot of strawberry increases the weight of, and there is generation in each cultivated strawberry area of China.The grey mould fruit rot of strawberry cause of disease is the botrytis cinerea of Deuteromycotina Botrytis, and this mycocriny natural disposition is strong, and host range is wide, and spore is elongated.Can cause harm respectively blade, petiole, anthocaulus, flower and fruit, especially obvious especially in berry maturing stage symptom, all be suitable for pathogen infection and diffusion in southern fruit picking peak period temp. and humidity especially, it is particularly serious to fall ill.In flower bud and when morbidity flower,, the initial stage water soaking mode point occurs at calyx, after be expanded into subcircular to irregular shape scab, sepal is withered.Disease expands on ovary and the young fruit, causes young fruit to rot.Disease infects painted berry, and the initial stage scab is filbert having one's hair waved shape, and the back forms the light brown color spot, expansion and grow canescence hair shape bacterium layer in the fruit rapidly later on, after to become the grey powdery mould, i.e. conidium, the soft corruption of final whole fruit comes off.The diseased fruit rate of susceptible variety is about 30%, and serious reaches more than 60%, very big to strawberry output, quality influence, has seriously restricted further developing that strawberry produces.
Present on the means of control grey mould fruit rot of strawberry, mainly still chemical control, agricultural chemicals commonly used mainly is bordeaux mixture, mancozeb, tpn, thiophanate methyl.Efficient, quick-acting, easy to use, economic benefit advantages of higher that bactericide has, but have only protective effect, do not have treatment and root out effect, and the lasting period to be short, application times is many, and germ has produced pesticide resistance to it; Long-time unreasonable use chemical pesticide control grey mould fruit rot of strawberry causes pathogen to develop immunity to drugs gradually, and control efficiency reduces, and can increase residue of pesticide in the fruit, and contaminated environment increases production cost.Chemical pesticide still is difficult to solve effectively to people and animals' toxic and side effect and residue problem so far.
Along with the appearance of more and more pesticide resistance phytopathogens, seek that other is alternative, toxic and side effect is little, agricultural chemicals has become the focal issue of agricultural sustainable development safely and effectively.Characteristics such as it is safe, efficient, pollution-free that botanical biological pesticide has and are preserved the ecological environment and food safety production requires to match.And by the biopesticide of making after the improvement of vegetable source natural compound, not only control efficiency is good, also to fruit, flower, the Ye Douwu harm of plant, to the person poultry safety, has efficient noresidue, no poisoning, advantages such as the non-resistant property of medicine; In the control disease, can effectively improve quality of agricultural product simultaneously.
Summary of the invention
Technical problem to be solved by this invention provides a kind of plant resource biocide preparation based on thymol, and said preparation is a kind of friendly preparation of many effects novel environmental that integrates antibiotic, nutrition, improves quality.
The technical problem that the present invention also will solve provides the preparation method of above-mentioned plant resource biocide preparation.
The technical problem that the present invention will solve at last provides the application of above-mentioned plant resource biocide preparation.
For solving the problems of the technologies described above, the technical solution used in the present invention is as follows:
A kind of plant resource biocide preparation, it comprises the component of following percetage by weight: thymol 0.1～50%, emulsifier 5～30%, azone 0.1～20%, ethanol 10～60%, water 0.1～84.8%.
Above-mentioned plant resource biocide preparation preferably comprises the component of following percetage by weight: thymol 0.5～5%, emulsifier 10～20%, azone 1～2%, ethanol 20～40%, water 33～68%.
Wherein, described emulsifier is one or more the mixture in phenethyl phenol polyethenoxy ether, phenethyl phenol polyoxyethylene poly-oxygen propylene aether, aralkyl phenol polyethenoxy ether phosphate, alkylphenol polyoxyethylene phosphate, alkyl naphthalene sulfonic acid calcium, castor oil ethylene oxide adduct, aralkyl phenol polyethenoxy ether, fatty alcohol-polyoxyethylene ether, alkyl phenol formaldehyde resin polyoxyethylene ether and the dialkyl benzene sulfonic acids calcium.
Above-mentioned thymol can carry out composite with carbendazim, Tebuconazole.
The preparation method of above-mentioned plant resource biocide preparation, press formula ratio and prepare raw material thymol, emulsifier, azone, second alcohol and water, thymol added mix the formation solution A in the part ethanol, again emulsifier and azone are formed solution B with the dissolving of residue ethanol, then solution A, solution B and water are mixed namely.
Wherein, ethanol uses (being above-mentioned part ethanol and above-mentioned residue ethanol) at twice, the abundant dissolving of each used in amounts raw material that assurance is dissolved, and twice consumption sum is the ethanol raw material total amount of formula ratio.
The application of above-mentioned plant resource biocide preparation in promoting the strawberry growth.
The application of above-mentioned plant resource biocide preparation in the control grey mould fruit rot of strawberry.
Beneficial effect: its effective component of plant resource biocide preparation based on thymol of the present invention derives from plant or its extract with insecticidal function, can not only effectively prevent and treat the crop gray mold, have simultaneously promote crop growth, increasing both production and income, improve quality, improve plant recovery of nutrient, pest control, multi-efficiency such as pollution-free, be applicable to nuisanceless, green and organic farm products production.The said preparation effect significantly, raw material are easy to get, and production technology is simple, market potential is big, economic benefit and social benefit are obvious, has very wide application prospect.
Embodiment
According to following embodiment, the present invention may be better understood.Yet, those skilled in the art will readily understand that embodiment is described only to be used for explanation the present invention, and should also can not limit the present invention described in detail in claims.
Embodiment 1:
A kind of plant resource biocide preparation, it comprises the component of following percetage by weight: thymol 2%, emulsifier (emulsifier is the phenethyl phenol polyethenoxy ether) 20%, azone 2%, ethanol 40%, water 36%.
The preparation method of above-mentioned plant resource biocide preparation, press formula ratio and prepare raw material thymol, emulsifier, azone, second alcohol and water, mix the formation solution A in the ethanol with thymol adding 1/4 amount, again emulsifier and azone are formed solution B with the ethanol dissolving that remains 3/4 amount, then solution A, solution B and water are mixed namely.
Embodiment 2:
A kind of plant resource biocide preparation, it comprises the component of following percetage by weight: thymol 1%, emulsifier (emulsifier is that emulsifier is aralkyl phenol polyethenoxy ether phosphate and alkylphenol polyoxyethylene phosphate by weight 1: 1 mixture) 10%, azone 2%, ethanol 20%, water 67%.
The preparation method of above-mentioned plant resource biocide preparation, press formula ratio and prepare raw material thymol, emulsifier, azone, second alcohol and water, mix the formation solution A in the ethanol with thymol adding 1/2 amount, again emulsifier and azone are formed solution B with the ethanol dissolving that remains 1/2 amount, then solution A, solution B and water are mixed namely.
Embodiment 3:
A kind of plant resource biocide preparation, it comprises the component of following percetage by weight: thymol 0.5%, emulsifier (emulsifier is the alkylphenol polyoxyethylene phosphate) 10%, azone 2%, ethanol 20%, water 67.5%.
The preparation method is with embodiment 2.
Embodiment 4:
A kind of plant resource biocide preparation, it comprises the component of following percetage by weight: thymol 0.1%, emulsifier (emulsifier is alkyl naphthalene sulfonic acid calcium and castor oil ethylene oxide adduct by weight 1: 2 mixture) 30%, azone 0.1%, ethanol 60%, water 9.8%.
The preparation method is with embodiment 2.
Embodiment 5:
A kind of plant resource biocide preparation, it comprises the component of following percetage by weight: thymol 50%, emulsifier (emulsifier is aralkyl phenol polyethenoxy ether, fatty alcohol-polyoxyethylene ether and alkyl phenol formaldehyde resin polyoxyethylene ether by weight 1: 2: 1 mixture) 5%, azone 20%, ethanol 10%, water 15%.
The preparation method is with embodiment 2.
Embodiment 6: based on the plant resource biocide preparation of thymol to the promotion of plant growth with to the improvement effect of quality.
Formula I: 1/2Hoagland nutrient solution.
The plant resource biocide preparation of the thymol that formula I I: embodiment 1 prepares.
Choose 3 processing of field piece design: handle 1: contrast (contrast CK is clear water); Handle 2: formula I; Handle 3: formula I I.Each is handled and repeats 3 times, and treatment fluid sprays once every 5d, sprays continuously 3 times, waits for that gathering in the crops the back sampling analyzes.
Assay method:
Vc measures: with reference to high friend's method, get about testing sample 2g, add a small amount of 2% hydrochloric acid, make homogenate in tissue mashing machine, move in the 100mL volumetric flask, to scale, absorbent cotton filters with 1% oxalic acid constant volume, get 10mL filtrate, with demarcated 2,6-dichloroindophenol solution titration determination Vc content.(high friend. the agrochemistry common analysis. Xi'an: Shaanxi science tech publishing house, 1980.)
Soluble solid is measured: adopt hand-held digital display saccharometer to measure.Concrete with reference to opening peaceful method mensuration.(to open peaceful. different proportioning matrix are yielded positive results to strawberry and the influence of fruit quality. Anhui agricultural science, 2011,39 (26): 15876-15877,15881.)
. table 1 different disposal is to the influence of strawberry biological amount and quality
As shown in Table 1, strawberry is handled back individual plant fruit number, single fruit weight, single plant yield, Vc content and soluble solid content in formula I has increased by 9.4%, 9.2%, 8.0%, 4.4% and 7.8% than contrast respectively; Formula I I handles back individual plant fruit number, single fruit weight, single plant yield, Vc content and soluble solid content has increased by 16.2%, 15.5%, 21.0%, 7.6% and 11.2% than contrast respectively.
On having as can be known, with formula I I handle to strawberry biological amount and quality to improve effect best.
Embodiment 7: based on the plant resource biocide preparation of thymol at the preventive and therapeutic effect to grey mould fruit rot of strawberry.
Formula I: 70% thiophanate methyl.
The plant resource biocide preparation of the thymol that formula I I: embodiment 1 prepares.
Choose three processing of morbidity field piece design: handle 1: contrast (contrast CK is clear water); Handle 2: formula I; Handle 3: formula I I.Each handle to repeat 3 times, and treatment fluid sprays once every 5d, carries out statistical analysis after spraying 3 times.
Assay method:
The mensuration of disease index and control efficiency: with reference to Dou Rui flitch method.(Dou Rui wood .3 plants endogenetic bacterium is to the control efficiency of graw mold of tomato. the Henan agricultural science, and 2010,4:77-79.)
As shown in Table 2, on strawberry, formula I is 45.99% to the preventive effect of grey mould fruit rot of strawberry; The preventive effect of the grey mould fruit rot of strawberry of formula I I is 84.47%.
As from the foregoing, handle best to the control efficiency of grey mould fruit rot of strawberry with formula I I.
Table 2 different disposal is to the grey mould fruit rot of strawberry control efficiency
Claims (1)
1. the application of plant resource preparation in improving strawberry output;
Wherein, described plant resource preparation, it comprises the component of following percetage by weight: thymol 2%, phenethyl phenol polyethenoxy ether 20%, azone 2%, ethanol 40%, water 36%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110400440 CN102388869B (en) | 2011-12-06 | 2011-12-06 | Botanical fungicide and its application in control of strawberry botrytis cinerea |
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|---|---|---|---|
| CN 201110400440 CN102388869B (en) | 2011-12-06 | 2011-12-06 | Botanical fungicide and its application in control of strawberry botrytis cinerea |
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| CN102388869A CN102388869A (en) | 2012-03-28 |
| CN102388869B true CN102388869B (en) | 2013-08-07 |
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| CN102365939B (en) * | 2011-12-06 | 2013-04-17 | 江苏省农业科学院 | Botanical sterilization preparation and application thereof for controlling anthracnose of grape |
| CN104026168B (en) * | 2014-05-28 | 2016-05-18 | 赵兰 | A kind of natural bactericidal agent of preventing and treating grey mould fruit rot of strawberry and preparation method thereof |
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| CN1357229A (en) * | 2000-12-13 | 2002-07-10 | 刘岱 | Application, production and processing of elsholtzia herb as natural plant pest and disease preventing and eliminating agent |
| AU2006209046A1 (en) * | 2005-01-26 | 2006-08-03 | Gavish-Galilee Bio Applications Ltd | Compositions and methods for protection of harvested fruits from decay |
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