CN104026168B - A kind of natural bactericidal agent of preventing and treating grey mould fruit rot of strawberry and preparation method thereof - Google Patents
A kind of natural bactericidal agent of preventing and treating grey mould fruit rot of strawberry and preparation method thereof Download PDFInfo
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- CN104026168B CN104026168B CN201410232816.7A CN201410232816A CN104026168B CN 104026168 B CN104026168 B CN 104026168B CN 201410232816 A CN201410232816 A CN 201410232816A CN 104026168 B CN104026168 B CN 104026168B
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Abstract
A kind of natural bactericidal agent of preventing and treating grey mould fruit rot of strawberry provided by the invention, is made up of following component: onion, garlic, lotus leaf, birthwort, Chinese honey locust, tarragon, Radix Isatidis, Pogostemon cablin, honeysuckle, Herba Andrographitis. The natural bactericidal agent raw material sources of this control grey mould fruit rot of strawberry are abundant, with low cost, adopt the component of pure natural to make, not only Nonpoisonous, non-environmental-pollution, and can effectively prevent and treat grey mould fruit rot of strawberry.
Description
Technical field
The invention belongs to prevention and elimination of disease and pests field, particularly a kind of natural bactericidal agent and preparation thereof that prevents and treats grey mould fruit rot of strawberryMethod.
Background technology
Grey mould fruit rot of strawberry StrawberryGrayMould grey mould fruit rot of strawberry is the important disease in current strawberry production. IThere is generation in the each cultivated strawberry of state area, and 20 century 70 sequela increase the weight of gradually, especially the southern fruit picking phase justIn the value spring rain time, fall ill even more serious. The generation of grey mould fruit rot of strawberry often causes flower and fruit rot, the disease of susceptible varietyFruit rate is in 30% left and right, and serious reaches more than 60%, affects very large on the mould output of grass, quality.
Grey mould fruit rot of strawberry mainly cause harm flower, leaf and fruit, also encroach on blade and petiole. Morbidity is many since the florescence, germAt first, from infecting opening the flower that loses or weaker position, make flower be light brown necrosis and rot, produce the mould layer of grey. Ye DuoInvade from base portion strong yellow limb edge, form " V " font brown spot, or the position of dropping along petal infects, form closely roundShape necrotic plaque, has on it and does not very significantly take turns line, the more sparse grey mold of upper life. Fruit is caught an illness many from residual petal or closeOr the position of kiss the earth starts, also can, from the position intrusion contacting with invalid tissue in early days, just be water stain shape taupe badExtremely, darken subsequently, fruit rot, surface produces the mould layer of dense grey. Petiole morbidity, be light brown necrosis,Drying shrinkage, produces sparse grey mold on it.
At present, in vegetables, melon and fruit plantation, be chemical pesticide bactericide for preventing and treating grey mould fruit rot of strawberry normally used, byThe problem of environmental pollution causing in a large amount of use chemical pesticides is on the rise. Meanwhile, adopt chemical pesticide bactericide, timeVarious phytopathogen bacterial classifications produce drug resistance, the resistance to the action of a drug, have increased the difficulty of control, even cause and prevent and treat unsuccessfully. ThisOutward, chemical pesticide, because it does not have selectively, also can threaten to useful biology. And a lot of chemical pesticides areNon-degradable or be difficult to degraded, easily pollutes the enrichment of thing at animals and plants cylinder accumulation.
Along with the public is more and more higher to the requirement of " pollution-free food ", " pollution-free food ", market to pollution-free, can be naturalThe ecological agricultural chemical demand of degraded also increases gradually. From the development of pesticide science, on using, pesticide manufacturing " returnsNature " and " Harmless " of agricultural chemicals, be the inexorable trend of society and Development of Natural Science, therefore new bio wide spectrumBactericide has wide development prospect.
Summary of the invention
Goal of the invention: the sky that the object of this invention is to provide a kind of environmental protection, control grey mould fruit rot of strawberry that bactericidal effect is goodSo bactericide.
Technical scheme: a kind of natural bactericidal agent of preventing and treating grey mould fruit rot of strawberry provided by the invention, made by following component: oceanGreen onion, garlic, lotus leaf, birthwort, Chinese honey locust, tarragon, Radix Isatidis, Pogostemon cablin, honeysuckle, Herba Andrographitis.
Component by following weight portion is made: onion 10-20 part, garlic 8-12 part, lotus leaf 8-12 part, birthwort 2-4Part, Chinese honey locust 2-4 part, tarragon 2-4 part, Radix Isatidis 2-4 part, Pogostemon cablin 1-3 part, honeysuckle 1-3 part, Herba Andrographitis1-3 part.
Component by following weight portion is made: 15 parts of onions, 10 parts, garlic, 10 parts, lotus leaf, 3 parts of birthworts, Chinese honey locust3 parts, 3 parts of tarragons, 3 parts of Radix Isatidis, 2 parts of Pogostemon cablin, 2 parts of honeysuckles, 2 parts of Herba Andrographitis.
The present invention also provides the preparation method of the natural bactericidal agent of above-mentioned control grey mould fruit rot of strawberry, comprises the following steps:
(1) raw material is mixed respectively after pulverizing, sieving, obtain compound; Described raw material be onion, garlic, lotus leaf,Birthwort, Chinese honey locust, tarragon, Radix Isatidis, Pogostemon cablin, honeysuckle and Herba Andrographitis;
(2) compound is added 5-10 doubly measure 95% ethanol water, be heated to 50-70 DEG C and decoct 2-4h;
(3) filter, filtrate is collected, and filter residue adds 5-10 doubly to measure 95% ethanol water, is heated to 50-70 DEG C and decoctsBoil 2-4h;
(4) filter, merging filtrate, is concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, quietPut 24-48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds the water-soluble solution of same weight, must prevent and treat the natural of grey mould fruit rot of strawberryBactericide.
The present invention also provides the application of the natural bactericidal agent of above-mentioned control grey mould fruit rot of strawberry, is specially: will prevent and treat strawberry ashThe natural bactericidal agent of mildew mixes and is sterilization microbial inoculum with 500-1000 times of water gaging.
Beneficial effect: the natural bactericidal agent raw material sources of control grey mould fruit rot of strawberry provided by the invention are abundant, with low cost,Adopt the component of pure natural to make, not only Nonpoisonous, non-environmental-pollution, and can effectively prevent and treat grey mould fruit rot of strawberry.
Detailed description of the invention
According to following embodiment, the present invention may be better understood. But, those skilled in the art will readily understand, realExecute the described concrete material proportion of example, process conditions and result thereof only for the present invention is described, and should also can notThe present invention described in detail in restriction claims.
Embodiment 1
The natural bactericidal agent of control grey mould fruit rot of strawberry, made by the component of following weight portion: 15 parts of onions, 10 parts, garlic,10 parts, lotus leaf, 3 parts of birthworts, 3 parts of Chinese honey locusts, 3 parts of tarragons, 3 parts of Radix Isatidis, 2 parts of Pogostemon cablin, honeysuckle 22 parts of part, Herba Andrographitis
Its preparation method, comprises the following steps:
(1) raw material is mixed respectively after pulverizing, sieving, obtain compound; Described raw material be onion, garlic, lotus leaf,Birthwort, Chinese honey locust, tarragon, Radix Isatidis, Pogostemon cablin, honeysuckle and Herba Andrographitis;
(2) compound is added 8 times measure 95% ethanol water, be heated to 60 DEG C and decoct 3h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 8 times of amounts 95%, is heated to 60 DEG C and decocts 2-4h;
(4) filter, merging filtrate, is concentrated into ethanol and adds 1/15 of total amount, adds the water of 2 times of volumes of concentrate,Leave standstill 36h;
(5) filter, filtrate is concentrated into without alcohol taste, adds the water-soluble solution of same weight, must prevent and treat the natural of grey mould fruit rot of strawberryBactericide.
Embodiment 2
The natural bactericidal agent of control grey mould fruit rot of strawberry, made by the component of following weight portion: 20 parts of onions, 8 parts, garlic,8 parts, lotus leaf, 2 parts of birthworts, 4 parts of Chinese honey locusts, 4 parts of tarragons, 4 parts of Radix Isatidis, 3 parts of Pogostemon cablin, 3 parts of honeysuckles,1 part of Herba Andrographitis.
Its preparation method, comprises the following steps:
(1) raw material is mixed respectively after pulverizing, sieving, obtain compound; Described raw material be onion, garlic, lotus leaf,Birthwort, Chinese honey locust, tarragon, Radix Isatidis, Pogostemon cablin, honeysuckle and Herba Andrographitis;
(2) compound is added 5 times measure 95% ethanol water, be heated to 70 DEG C and decoct 4h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5 times of amounts 95%, is heated to 70 DEG C and decocts 4h;
(4) filter, merging filtrate, is concentrated into ethanol and adds 1/20 of total amount, adds the water of 1 times of volume of concentrate,Leave standstill 48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds the water-soluble solution of same weight, must prevent and treat the natural of grey mould fruit rot of strawberryBactericide.
Embodiment 3
The natural bactericidal agent of control grey mould fruit rot of strawberry, made by the component of following weight portion: 10 parts of onions, 12 parts, garlic,12 parts, lotus leaf, 4 parts of birthworts, 2 parts of Chinese honey locusts, 2 parts of tarragons, 2 parts of Radix Isatidis, 1 part of Pogostemon cablin, honeysuckle 13 parts of part, Herba Andrographitis.
Its preparation method, comprises the following steps:
(1) raw material is mixed respectively after pulverizing, sieving, obtain compound; Described raw material be onion, garlic, lotus leaf,Birthwort, Chinese honey locust, tarragon, Radix Isatidis, Pogostemon cablin, honeysuckle and Herba Andrographitis;
(2) compound is added 10 times measure 95% ethanol water, be heated to 50 DEG C and decoct 2h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 10 times of amounts 95%, is heated to 50 DEG C and decocts 2h;
(4) filter, merging filtrate, is concentrated into ethanol and adds 1/10 of total amount, adds the water of 3 times of volumes of concentrate,Leave standstill 24h;
(5) filter, filtrate is concentrated into without alcohol taste, adds the water-soluble solution of same weight, must prevent and treat the natural of grey mould fruit rot of strawberryBactericide.
The inhibition activity of the natural bactericidal agent of control grey mould fruit rot of strawberry that embodiment 4 measures embodiment 1 to 3 to strawberry pathogen
This experiment is in vitro, and the natural bactericidal agent of the control grey mould fruit rot of strawberry of mensuration embodiment 1 to 3 is to grassThe inhibition activity of certain kind of berries pathogen, method and result are as follows:
1. for examination bacterial classification
Botrytiscinerea is commercially available for Botrytis cinerea germ.
2. Toxicity Determination
Utilize mycelial growth rate method and spore germination method to measure the embodiment of the present invention 1 natural bactericidal agent to Botrytis cinerea germThe virulence of Botrytiscinerea, calculate inhibiting rate by following formula:
3. experimental result
Measure embodiment 1-3 natural bactericidal agent to the virulence for examination pathogen with growth rate method, the results are shown in Table 1.
Table 1 embodiment 1-3 natural bactericidal agent is to the virulence for examination pathogen
Adopt the method for regulation in " pesticide field efficacy medicine test criterion ", to the natural disinfection of the embodiment of the present invention 1 to 3The field efficacy of agent is tested, and diluted concentration is 500-1000 times, the results are shown in Table 2.
The field control effectiveness test of the natural bactericidal agent of table 2 embodiment 1 to 3
The natural bactericidal agent treatment grey mould fruit rot of strawberry that adopts embodiment 1 to 3, various diseases is respectively treated 100 strains, and dilution is doublyNumber is 500 times, the results are shown in Table 3.
The natural bactericidal agent treatment grey mould fruit rot of strawberry of table 3 embodiment 1 to 3
Claims (3)
1. a natural bactericidal agent of preventing and treating grey mould fruit rot of strawberry, is characterized in that: the component by following weight portion is made: onion 10-20 part, garlic 8-12 part, lotus leaf 8-12 part, birthwort 2-4 part, Chinese honey locust 2-4 part, tarragon 2-4 part, Radix Isatidis 2-4 part, Pogostemon cablin 1-3 part, honeysuckle 1-3 part, Herba Andrographitis 1-3 part; The preparation method of the natural bactericidal agent of described control grey mould fruit rot of strawberry, comprises the following steps:
(1) raw material is mixed respectively after pulverizing, sieving, obtain compound; Described raw material is onion, garlic, lotus leaf, birthwort, Chinese honey locust, tarragon, Radix Isatidis, Pogostemon cablin, honeysuckle and Herba Andrographitis;
(2) compound is added 5-10 doubly measure 95% ethanol water, be heated to 50-70 DEG C and decoct 2-4h;
(3) filter, filtrate is collected, and filter residue adds 5-10 doubly to measure 95% ethanol water, is heated to 50-70 DEG C and decocts 2-4h;
(4) filter, merging filtrate, is concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, leaves standstill 24-48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds the water-soluble solution of same weight, must prevent and treat the natural bactericidal agent of grey mould fruit rot of strawberry.
2. a kind of natural bactericidal agent of preventing and treating grey mould fruit rot of strawberry according to claim 1, is characterized in that: the component by following weight portion is made: 15 parts of onions, 10 parts, garlic, 10 parts, lotus leaf, 3 parts of birthworts, 3 parts of Chinese honey locusts, 3 parts of tarragons, 3 parts of Radix Isatidis, 2 parts of Pogostemon cablin, 2 parts of honeysuckles, 2 parts of Herba Andrographitis.
3. a preparation method for the natural bactericidal agent of the control grey mould fruit rot of strawberry described in claim 1 or 2, is characterized in that: comprise the following steps:
(1) raw material is mixed respectively after pulverizing, sieving, obtain compound; Described raw material is onion, garlic, lotus leaf, birthwort, Chinese honey locust, tarragon, Radix Isatidis, Pogostemon cablin, honeysuckle and Herba Andrographitis;
(2) compound is added 5-10 doubly measure 95% ethanol water, be heated to 50-70 DEG C and decoct 2-4h;
(3) filter, filtrate is collected, and filter residue adds 5-10 doubly to measure 95% ethanol water, is heated to 50-70 DEG C and decocts 2-4h;
(4) filter, merging filtrate, is concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, leaves standstill 24-48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds the water-soluble solution of same weight, must prevent and treat the natural bactericidal agent of grey mould fruit rot of strawberry.
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| CN105053075A (en) * | 2015-07-20 | 2015-11-18 | 句容市后白镇解氏家庭农场 | Natural bactericide for controlling loquat leaf parch blight disease and preparation method of natural bactericide |
| CN106106587A (en) * | 2016-06-22 | 2016-11-16 | 固镇玉鹏生态农业文化园有限公司 | A kind of grey mould fruit rot of strawberry control agent and preparation method thereof |
| CN105994451A (en) * | 2016-07-29 | 2016-10-12 | 广西素安生态农业有限公司 | Chinese herbal medicine control agent for common diseases of strawberries and preparation method thereof |
| CN106258363A (en) * | 2016-08-11 | 2017-01-04 | 江苏省农业科学院 | A kind of Fructus Fragariae Ananssae nursery and Bulbus Allii interplanting cultural method |
| CN107027817A (en) * | 2017-05-03 | 2017-08-11 | 沈荣存 | A kind of preparation method for the natural bactericidal agent for preventing and treating reed wormwood artemisia gray mold |
| CN107027818A (en) * | 2017-05-03 | 2017-08-11 | 沈荣存 | A kind of natural bactericidal agent for preventing and treating reed wormwood artemisia gray mold and its application |
| CN107568274A (en) * | 2017-10-31 | 2018-01-12 | 桐梓县茅石乡龙会村蔬菜种植场 | It is a kind of to be used to prevent and treat botanical fungicide of grey mould fruit rot of strawberry and preparation method thereof |
| CN109169716A (en) * | 2018-07-27 | 2019-01-11 | 普定绿盛园生态农业有限公司 | A kind of composition and preparation method thereof for preventing and treating grey mould fruit rot of strawberry |
| CN110250219B (en) * | 2019-08-05 | 2021-09-10 | 深圳大学 | New application of patchouli volatile oil |
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| CN102388869A (en) * | 2011-12-06 | 2012-03-28 | 江苏省农业科学院 | Botanical fungicide and its application in control of strawberry botrytis cinerea |
| CN102409001A (en) * | 2011-11-01 | 2012-04-11 | 浙江省农业科学院 | Fungus used for performing biological prevention and control on plant soil-borne diseases |
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| CN1327745A (en) * | 2001-07-04 | 2001-12-26 | 孙树贵 | Agricultural chemical for preventing and treating plant botrytis |
| CN102409001A (en) * | 2011-11-01 | 2012-04-11 | 浙江省农业科学院 | Fungus used for performing biological prevention and control on plant soil-borne diseases |
| CN102388869A (en) * | 2011-12-06 | 2012-03-28 | 江苏省农业科学院 | Botanical fungicide and its application in control of strawberry botrytis cinerea |
| WO2013110258A1 (en) * | 2012-01-24 | 2013-08-01 | Hochschule Anhalt | Antifungal formulations for combatting plant diseases |
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