CN105053075A - Natural bactericide for controlling loquat leaf parch blight disease and preparation method of natural bactericide - Google Patents
Natural bactericide for controlling loquat leaf parch blight disease and preparation method of natural bactericide Download PDFInfo
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- CN105053075A CN105053075A CN201510427517.3A CN201510427517A CN105053075A CN 105053075 A CN105053075 A CN 105053075A CN 201510427517 A CN201510427517 A CN 201510427517A CN 105053075 A CN105053075 A CN 105053075A
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Abstract
The invention provides a natural bactericide for controlling the loquat leaf parch blight disease. The natural bactericide is prepared from components as follows: spreading hedyotis herbs, garlic, kudzuvine roots, Tokyo violet herbs, Indian trumpetflower seeds, emilia herbs, coral ardisia roots, flowers of fragrant plantain lily, Actinidia chinensis roots and wild chrysanthemum flowers. According to the natural bactericide for controlling the loquat leaf parch blight disease, raw material sources are rich, the cost is low, and the natural bactericide is prepared from the pure natural components, is non-toxic and pollution-free and can effectively control the loquat leaf parch blight disease.
Description
Technical field
The invention belongs to prevention and elimination of disease and pests field, particularly a kind ofly prevent and treat natural bactericidal agent of the shrivelled disease of loquat blade tip and preparation method thereof.
Background technology
Loquat, another name: reed tangerine, golden ball, Lu Zhi, the rose family, Eriobotrya, loquat originates in southeast China, and because leaf-shaped is like Chinese lute musical instrument and name, its flower can be used as medicine.The height of tree 3 ~ 5 meters, leaf is large and grow, thick and have fine hair, and in oblong, shape is as Chinese lute.Another name is again reed tangerine, Lu Zhi, golden ball, scorching fruit, Jiao Zi.Loquat is different from most of fruit tree, and in autumn or bloom early winter, fruit is ripe in spring to early summer, all more Zao than other fruit, is therefore " solely for seasonal pathogens,exopathogens person in fruit tree " by title.The flower of loquat is white or faint yellow, has five pieces of petals, diameter about 2 centimetres, a branch of with five to ten one-tenth, can as nectar source crop.
The shrivelled disease of loquat blade tip is the common disease of loquat blade, is fungal disease, leaf occurs in blade face at the beginning of scab, in chlorisis little Huang point, continues and transfers little foxiness, foxiness to russet spot.Lesion size 1-3 millimeter, subcircular, horn shape or irregular shape.Later stage disease portion is through leaf two sides, and blade face scab central authorities look takes off for ash is brown, grows the mould shot-like particle of black, is cause of disease conidium and stroma.The maximum feature of this scab is that outer rim has a yellow halo, green blade face seems especially eye-catching.
At present, in vegetables, melon and fruit plantation, be chemical pesticide bactericide for preventing fungi normally used, the problem of environmental pollution caused owing to using chemical pesticide is in a large number on the rise.Meanwhile, adopt chemical pesticide bactericide, time various phytopathogen bacterial classification produce drug resistance, pesticide resistance, add the difficulty of control, even cause and prevent and treat unsuccessfully.In addition, chemical pesticide does not have selectivity because of it, also can threaten to useful biology.And a lot of chemical pesticide is non-degradable or is difficult to degraded, easily pollutes the enrichment of thing at animals and plants cylinder accumulation.
Along with the requirement of the public to " pollution-free food ", " pollution-free food " is more and more higher, market to pollution-free, can the ecological agricultural chemical demand of natural degradation also increase gradually.From the development of pesticide science, " Harmless " of " back to nature " and agricultural chemicals on pesticide manufacturing uses, be the inexorable trend of society and Development of Natural Science, therefore new bio wide-spectrum bactericide has wide development prospect.
Summary of the invention
Goal of the invention: the natural bactericidal agent that the object of this invention is to provide a kind of environmental protection, the shrivelled disease of control loquat blade tip that bactericidal effect is good.
Technical scheme: a kind of natural bactericidal agent preventing and treating the shrivelled disease of loquat blade tip provided by the invention, is made up of following component: Hedyotidis Diffusae, garlic, the root of kudzu vine, Chinese violet, Oroxylum indicum, sowthistle tasselflower herb, Japanese ardisia, beautiful Bamboo-utensil flower, Chinese actinidia root, mother chrysanthemum.
Be made up of the component of following weight portion: Hedyotidis Diffusae 8-12 part, garlic 8-12 part, root of kudzu vine 8-12 part, Chinese violet 2-4 part, Oroxylum indicum 2-4 part, sowthistle tasselflower herb 1-3 part, Japanese ardisia 1-3 part, Yu Bamboo-utensil flower 1-3 part, Chinese actinidia root 0.5-1.5 part, mother chrysanthemum 0.5-1.5 part.
Be made up of the component of following weight portion: Hedyotidis Diffusae 10 parts, 10 parts, garlic, the root of kudzu vine 10 parts, Chinese violet 3 parts, Oroxylum indicum 3 parts, sowthistle tasselflower herb 2 parts, Japanese ardisia 2 parts of, Yu Bamboo-utensil spend 2 parts, Chinese actinidia root 1 part, mother chrysanthemum 1 part.
Present invention also offers the preparation method of the natural bactericidal agent of the shrivelled disease of above-mentioned control loquat blade tip, comprise the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is Hedyotidis Diffusae, garlic, the root of kudzu vine, Chinese violet, Oroxylum indicum, sowthistle tasselflower herb, Japanese ardisia, Yu Bamboo-utensil flower, Chinese actinidia root and mother chrysanthemum;
(2) compound is added the ethanol water of 5-10 times amount 95%, be heated to 50-70 DEG C and decoct 2-4h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5-10 times amount 95%, is heated to 50-70 DEG C and decocts 2-4h;
(4) filter, merging filtrate, is concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, leaves standstill 24-48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of the shrivelled disease of loquat blade tip.
Present invention also offers the application of the natural bactericidal agent of the shrivelled disease of above-mentioned control loquat blade tip, be specially: the natural bactericidal agent of the shrivelled disease of control loquat blade tip is mixed with 500-1000 times of water gaging and is sterilization microbial inoculum.
Beneficial effect: the natural bactericidal agent abundant raw material source of the shrivelled disease of control loquat blade tip provided by the invention, with low cost, adopt the component of pure natural to make, not only Nonpoisonous, non-environmental-pollution, and effectively can prevent and treat the shrivelled disease of loquat blade tip.
Embodiment
According to following embodiment, the present invention may be better understood.But those skilled in the art will readily understand, concrete material proportion, process conditions and result thereof described by embodiment only for illustration of the present invention, and should can not limit the present invention described in detail in claims yet.
Embodiment 1
The natural bactericidal agent of control loquat blade tip shrivelled disease, be made up of the component of following weight portion: Hedyotidis Diffusae 10 parts, 10 parts, garlic, the root of kudzu vine 4 parts, Chinese violet 2 parts, Oroxylum indicum 2 parts, sowthistle tasselflower herb 1 part, Japanese ardisia 3 parts, beautiful Bamboo-utensil spend 3 parts, Chinese actinidia root 1.5 parts, mother chrysanthemum 0.5 part.
Its preparation method, comprises the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is Hedyotidis Diffusae, garlic, the root of kudzu vine, Chinese violet, Oroxylum indicum, sowthistle tasselflower herb, Japanese ardisia, Yu Bamboo-utensil flower, Chinese actinidia root and mother chrysanthemum;
(2) compound is added the ethanol water of 8 times amount 95%, be heated to 60 DEG C and decoct 3h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 8 times amount 95%, is heated to 60 DEG C and decocts 2-4h;
(4) filter, merging filtrate, be concentrated into that ethanol adds total amount 1/15, add the water of concentrate 2 times of volumes, leave standstill 36h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of the shrivelled disease of loquat blade tip.
Embodiment 2
The natural bactericidal agent of control loquat blade tip shrivelled disease, be made up of the component of following weight portion: Hedyotidis Diffusae 8 parts, 12 parts, garlic, the root of kudzu vine 6 parts, Chinese violet 4 parts, Oroxylum indicum 4 parts, sowthistle tasselflower herb 3 parts, Japanese ardisia 1 part, beautiful Bamboo-utensil spend 1 part, Chinese actinidia root 0.5 part, mother chrysanthemum 1.5 parts.
Its preparation method, comprises the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is Hedyotidis Diffusae, garlic, the root of kudzu vine, Chinese violet, Oroxylum indicum, sowthistle tasselflower herb, Japanese ardisia, Yu Bamboo-utensil flower, Chinese actinidia root and mother chrysanthemum;
(2) compound is added the ethanol water of 5 times amount 95%, be heated to 70 DEG C and decoct 4h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5 times amount 95%, is heated to 70 DEG C and decocts 4h;
(4) filter, merging filtrate, be concentrated into that ethanol adds total amount 1/20, add the water of concentrate 1 times of volume, leave standstill 48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of the shrivelled disease of loquat blade tip.
Embodiment 3
The natural bactericidal agent of control loquat blade tip shrivelled disease, be made up of the component of following weight portion: Hedyotidis Diffusae 12 parts, 8 parts, garlic, the root of kudzu vine 5 parts, Chinese violet 3 parts, Oroxylum indicum 3 parts, sowthistle tasselflower herb 2 parts, Japanese ardisia 2 parts, beautiful Bamboo-utensil spend 2 parts, Chinese actinidia root 1 part, mother chrysanthemum 1 part.
Its preparation method, comprises the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is Hedyotidis Diffusae, garlic, the root of kudzu vine, Chinese violet, Oroxylum indicum, sowthistle tasselflower herb, Japanese ardisia, Yu Bamboo-utensil flower, Chinese actinidia root and mother chrysanthemum;
(2) compound is added the ethanol water of 10 times amount 95%, be heated to 50 DEG C and decoct 2h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 10 times amount 95%, is heated to 50 DEG C and decocts 2h;
(4) filter, merging filtrate, be concentrated into that ethanol adds total amount 1/10, add the water of concentrate 3 times of volumes, leave standstill 24h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of the shrivelled disease of loquat blade tip.
Embodiment 4 measures the natural bactericidal agent of the shrivelled disease of control loquat blade tip of embodiment 1 to 3 to the inhibit activities of loquat pathogen
This experiment, in vitro, measures the natural bactericidal agent of the shrivelled disease of control loquat blade tip of embodiment 1 to 3 to the inhibit activities of loquat pathogen, method and result as follows:
1. for examination bacterial classification
Shrivelled germ Cylindrocladiumquinqueseptatum is commercially available.
2. Toxicity Determination
Mycelial growth rate method and spore germination method is utilized to measure the virulence of the embodiment of the present invention 1 natural bactericidal agent focusing rot bacterium Cylindrocladiumquinqueseptatum, by following formulae discovery inhibiting rate:
3. experimental result
Measure embodiment 1-3 natural bactericidal agent to the virulence for examination pathogen with growth rate method, the results are shown in Table 1.
Table 1 embodiment 1-3 natural bactericidal agent is to the virulence for examination pathogen
Adopt the method for regulation in " pesticide field efficacy medicine test criterion ", test the field efficacy of the natural bactericidal agent of the embodiment of the present invention 1 to 3, diluted concentration is 500-1000 times, the results are shown in Table 2.
The field control effectiveness test of the natural bactericidal agent of table 2 embodiment 1 to 3
Adopt the shrivelled disease of natural bactericidal agent treatment loquat blade tip of embodiment 1 to 3, various disease respectively treats 100 strains, and extension rate is 500 times, the results are shown in Table 3.
The shrivelled disease of natural bactericidal agent treatment loquat blade tip of table 3 embodiment 1 to 3
Claims (5)
1. prevent and treat a natural bactericidal agent for the shrivelled disease of loquat blade tip, it is characterized in that: be made up of following component: Hedyotidis Diffusae, garlic, the root of kudzu vine, Chinese violet, Oroxylum indicum, sowthistle tasselflower herb, Japanese ardisia, beautiful Bamboo-utensil flower, Chinese actinidia root, mother chrysanthemum.
2. a kind of natural bactericidal agent preventing and treating the shrivelled disease of loquat blade tip according to claim 1, is characterized in that: be made up of the component of following weight portion: Hedyotidis Diffusae 8-12 part, garlic 8-12 part, root of kudzu vine 8-12 part, Chinese violet 2-4 part, Oroxylum indicum 2-4 part, sowthistle tasselflower herb 1-3 part, Japanese ardisia 1-3 part, beautiful Bamboo-utensil flower 1-3 part, Chinese actinidia root 0.5-1.5 part, mother chrysanthemum 0.5-1.5 part.
3. a kind of natural bactericidal agent preventing and treating the shrivelled disease of loquat blade tip according to claim 1, is characterized in that: be made up of the component of following weight portion: Hedyotidis Diffusae 10 parts, 10 parts, garlic, the root of kudzu vine 10 parts, Chinese violet 3 parts, Oroxylum indicum 3 parts, sowthistle tasselflower herb 2 parts, Japanese ardisia 2 parts, beautiful Bamboo-utensil spend 2 parts, Chinese actinidia root 1 part, mother chrysanthemum 1 part.
4. prevent and treat a preparation method for the natural bactericidal agent of the shrivelled disease of loquat blade tip, it is characterized in that: comprise the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is Hedyotidis Diffusae, garlic, the root of kudzu vine, Chinese violet, Oroxylum indicum, sowthistle tasselflower herb, Japanese ardisia, Yu Bamboo-utensil flower, Chinese actinidia root and mother chrysanthemum;
(2) compound is added the ethanol water of 5-10 times amount 95%, be heated to 50-70 DEG C and decoct 2-4h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5-10 times amount 95%, is heated to 50-70 DEG C and decocts 2-4h;
(4) filter, merging filtrate, is concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, leaves standstill 24-48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of the shrivelled disease of loquat blade tip.
5. prevent and treat the application of natural bactericidal agent in the shrivelled disease of control loquat blade tip of the shrivelled disease of loquat blade tip, it is characterized in that: the natural bactericidal agent of the shrivelled disease of control loquat blade tip is mixed with 500-1000 times of water gaging and is sterilization microbial inoculum.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510427517.3A CN105053075A (en) | 2015-07-20 | 2015-07-20 | Natural bactericide for controlling loquat leaf parch blight disease and preparation method of natural bactericide |
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| CN201510427517.3A CN105053075A (en) | 2015-07-20 | 2015-07-20 | Natural bactericide for controlling loquat leaf parch blight disease and preparation method of natural bactericide |
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| CN111802379A (en) * | 2020-06-23 | 2020-10-23 | 中国农业科学院作物科学研究所 | Method for storing test-tube plantlets of Hosta plantaginea |
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Application publication date: 20151118 |
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