CN103340218A - Natural bactericide for prevention and treatment of peach brown rot and preparation method thereof - Google Patents
Natural bactericide for prevention and treatment of peach brown rot and preparation method thereof Download PDFInfo
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- CN103340218A CN103340218A CN2013102164092A CN201310216409A CN103340218A CN 103340218 A CN103340218 A CN 103340218A CN 2013102164092 A CN2013102164092 A CN 2013102164092A CN 201310216409 A CN201310216409 A CN 201310216409A CN 103340218 A CN103340218 A CN 103340218A
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- brown rot
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- peach brown
- bactericidal agent
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Abstract
A natural bactericide for prevention and treatment of peach brown rot is provided. The natural bactericide is prepared from the following components: balsam pear, phragmites australis, radix puerariae, aristolochia debilis, rhizoma atractylodis macrocephalae, polygonum cuspidatum, java brucea fruit, pogostemon cablin, belamcanda chinensis, and pyrola rotundifolia. The natural bactericide for prevention and treatment of peach brown rot has rich raw material sources, and is low in cost; and the natural bactericide is prepared by the pure natural components, so that the natural bactericide not only is non-toxic and pollution-free, but also can effectively prevent and treat peach brown rot.
Description
Technical field
The invention belongs to the prevention and elimination of disease and pests field, particularly a kind of natural bactericidal agent of preventing and treating the peach brown rot and preparation method thereof.
Background technology
The peach brown rot claims stalk break, fruit rot again, is the important fruit disease of peach.The symptom of the peach brown rot peach fruit of mainly causing harm, secondly also can cause harm flower, leaf and young sprout.Fruit can both be injured from the young fruit to the mellow fruit, but that more near maturation is injured is more heavy.The fruit morbidity produces the circular stigma of brown at the fruit face at first, if condition is suitable, scab will extend to full fruit in several days, and pulp is browning, soft corruption thereupon also, bears the mould clump of taupe at last on the scab surface, i.e. the acervulus of germ.After sick fruit was rotted, what have came off, and the dehydration drying shrinkage that has becomes mummy to hang branch to go up prolongedly not fall.Menophania hole skin is invaded fruit, and the later stage is mainly invaded fruits by various wounds.If the moth fruit is serious, and humidity is excessive, the peach brown rot permanent current is capable to cause disaster.Moist low temperature of florescence, morbidity forms spends corruption.
At present, in the plantation of vegetables, melon and fruit, for prevent fungi normally used be the chemical pesticide bactericide because the problem of environmental pollutions that a large amount of use chemical pesticides cause are on the rise.Simultaneously, adopt the chemical pesticide bactericide, the time various phytopathogen bacterial classifications produce drug resistances, pesticide resistance, increased the difficulty of control, even caused the control failure.In addition, chemical pesticide does not have selectivity because of it, can threaten to useful biology yet.And a lot of chemical pesticides are non-degradables or are difficult to degrade, and pollute the enrichment of thing easily at the animals and plants cylinder accumulation.
Along with the public is more and more higher to the requirement of " pollution-free food ", " pollution-free food ", but also increase gradually to the ecological agricultural chemical demand of pollution-free natural degradation in market.From the development of pesticide science, " nuisancelessization " of " back to nature " and agricultural chemicals be the inexorable trend of society and natural science development, so the new bio wide-spectrum bactericide has the wide development application prospect on pesticide manufacturing uses.
Summary of the invention
Goal of the invention: the natural bactericidal agent that the purpose of this invention is to provide a kind of environmental protection, control peach brown rot that bactericidal effect is good.
Technical scheme: a kind of natural bactericidal agent of preventing and treating the peach brown rot provided by the invention, made by following component: balsam pear, reed, the root of kudzu vine, birthwort, the bighead atractylodes rhizome, giant knotweed, Java brucea, Pogostemon cablin, blackberry lily, wintergreen.
Component by following weight portion is made: balsam pear 4-6 part, reed 4-6 part, root of kudzu vine 4-6 part, birthwort 2-4 part, bighead atractylodes rhizome 2-4 part, giant knotweed 1-3 part, Java brucea 1-3 part, Pogostemon cablin 1-3 part, blackberry lily 0.5-1.5 part, wintergreen 0.5-1.5 part.
Component by following weight portion is made: 5 parts in balsam pear, 5 parts in reed, 5 parts of the roots of kudzu vine, 3 parts of birthworts, 3 parts of the bighead atractylodes rhizomes, 2 parts of giant knotweeds, 2 parts of Java brucea, 2 parts of Pogostemon cablin, 1 part of blackberry lily, 1 part of wintergreen.
The present invention also provides the preparation method of the natural bactericidal agent of above-mentioned control peach brown rot, may further comprise the steps:
(1) with raw material mixing after pulverizing, sieving respectively, gets compound; Described raw material is balsam pear, reed, the root of kudzu vine, birthwort, the bighead atractylodes rhizome, giant knotweed, Java brucea, Pogostemon cablin, blackberry lily and wintergreen;
(2) compound is added 5-10 and doubly measure 95% ethanol water, be heated to 50-70 ℃ and decoct 2-4h;
(3) filter, filtrate is collected, and filter residue adds 5-10 and doubly measures 95% ethanol water, is heated to 50-70 ℃ and decocts 2-4h;
(4) filter, merging filtrate is concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, leaves standstill 24-48h;
(5) filter, filtrate is concentrated into does not have the alcohol flavor, adds with the water-soluble solution of weight, namely gets the natural bactericidal agent of control peach brown rot.
The present invention also provides the application of the natural bactericidal agent of above-mentioned control peach brown rot, is specially: the natural bactericidal agent that will prevent and treat the peach brown rot mixes with 500-1000 times of water gaging and is the sterilization microbial inoculum.
Beneficial effect: the natural bactericidal agent raw material sources of control peach brown rot provided by the invention are abundant, and are with low cost, adopt the component of pure natural to make, Nonpoisonous, non-environmental-pollution not only, and can effectively prevent and treat the peach brown rot.
Embodiment
According to following embodiment, the present invention may be better understood.Yet, those skilled in the art will readily understand that the described concrete material proportion of embodiment, process conditions and result thereof only are used for explanation the present invention, and should also can not limit the present invention described in detail in claims.
Embodiment 1
The natural bactericidal agent of control peach brown rot is made by the component of following weight portion: 4 parts in balsam pear, 6 parts in reed, 4 parts of the roots of kudzu vine, 2 parts of birthworts, 2 parts of the bighead atractylodes rhizomes, 1 part of giant knotweed, 3 parts of Java brucea, 3 parts of Pogostemon cablin, 1.5 parts of blackberry lilys, 0.5 part of wintergreen.
Its preparation method may further comprise the steps:
(1) with raw material mixing after pulverizing, sieving respectively, gets compound; Described raw material is balsam pear, reed, the root of kudzu vine, birthwort, the bighead atractylodes rhizome, giant knotweed, Java brucea, Pogostemon cablin, blackberry lily and wintergreen;
(2) compound is added 8 times and measure 95% ethanol water, be heated to 60 ℃ and decoct 3h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 8 times of amounts 95%, is heated to 60 ℃ and decocts 2-4h;
(4) filter, merging filtrate is concentrated into ethanol and adds 1/15 of total amount, adds the water of 2 times of volumes of concentrate, leaves standstill 36h;
(5) filter, filtrate is concentrated into does not have the alcohol flavor, adds with the water-soluble solution of weight, namely gets the natural bactericidal agent of control peach brown rot.
Embodiment 2
The natural bactericidal agent of control peach brown rot is made by the component of following weight portion: 6 parts in balsam pear, 4 parts in reed, 6 parts of the roots of kudzu vine, 4 parts of birthworts, 4 parts of the bighead atractylodes rhizomes, 3 parts of giant knotweeds, 1 part of Java brucea, 1 part of Pogostemon cablin, 0.5 part of blackberry lily, 1.5 parts of wintergreens.
Its preparation method may further comprise the steps:
(1) with raw material mixing after pulverizing, sieving respectively, gets compound; Described raw material is balsam pear, reed, the root of kudzu vine, birthwort, the bighead atractylodes rhizome, giant knotweed, Java brucea, Pogostemon cablin, blackberry lily and wintergreen;
(2) compound is added 5 times and measure 95% ethanol water, be heated to 70 ℃ and decoct 4h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5 times of amounts 95%, is heated to 70 ℃ and decocts 4h;
(4) filter, merging filtrate is concentrated into ethanol and adds 1/20 of total amount, adds the water of 1 times of volume of concentrate, leaves standstill 48h;
(5) filter, filtrate is concentrated into does not have the alcohol flavor, adds with the water-soluble solution of weight, namely gets the natural bactericidal agent of control peach brown rot.
Embodiment 3
The natural bactericidal agent of control peach brown rot is made by the component of following weight portion: 5 parts in balsam pear, 5 parts in reed, 5 parts of the roots of kudzu vine, 3 parts of birthworts, 3 parts of the bighead atractylodes rhizomes, 2 parts of giant knotweeds, 2 parts of Java brucea, 2 parts of Pogostemon cablin, 1 part of blackberry lily, 1 part of wintergreen.
Its preparation method may further comprise the steps:
(1) with raw material mixing after pulverizing, sieving respectively, gets compound; Described raw material is balsam pear, reed, the root of kudzu vine, birthwort, the bighead atractylodes rhizome, giant knotweed, Java brucea, Pogostemon cablin, blackberry lily and wintergreen;
(2) compound is added 10 times and measure 95% ethanol water, be heated to 50 ℃ and decoct 2h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 10 times of amounts 95%, is heated to 50 ℃ and decocts 2h;
(4) filter, merging filtrate is concentrated into ethanol and adds 1/10 of total amount, adds the water of 3 times of volumes of concentrate, leaves standstill 24h;
(5) filter, filtrate is concentrated into does not have the alcohol flavor, adds with the water-soluble solution of weight, namely gets the natural bactericidal agent of control peach brown rot.
The natural bactericidal agent of the control peach brown rot of embodiment 4 mensuration embodiment 1 to 3 is to the inhibition activity of peach pathogen
This experiment is under isolated condition, and the natural bactericidal agent of the control peach brown rot of mensuration embodiment 1 to 3 is to the inhibition activity of peach pathogen, and method and result are as follows:
1. for the examination bacterial classification
It is commercially available that fruit gives birth to chain sclerotinia sclerotiorum (Moniliunia fructicola).
2. Toxicity Determination
Utilize mycelial growth rate method and spore germination method to measure the embodiment of the invention 1 natural bactericidal agent to the virulence of the living chain sclerotinia sclerotiorum of fruit (Moniliunia fructicola), calculate inhibiting rate by following formula:
3. experimental result
Measure embodiment 1-3 natural bactericidal agent to the virulence for the examination pathogen with growth rate method, the results are shown in Table 1.
Table 1 embodiment 1-3 natural bactericidal agent is to the virulence for the examination pathogen
Adopt method of regulation in " pesticide field efficacy medicine test criterion ", the field efficacy of the natural bactericidal agent of the embodiment of the invention 1 to 3 tested, diluted concentration be 500-1000 doubly, the results are shown in Table 2.
The field control effectiveness test of the natural bactericidal agent of table 2 embodiment 1 to 3
Adopt the natural bactericidal agent treatment peach brown rot of embodiment 1 to 3, various diseases is respectively treated 100 strains, and extension rate is 500 times, the results are shown in Table 3.
The natural bactericidal agent treatment peach brown rot of table 3 embodiment 1 to 3
Claims (5)
1. a natural bactericidal agent of preventing and treating the peach brown rot is characterized in that: made by following component: balsam pear, reed, the root of kudzu vine, birthwort, the bighead atractylodes rhizome, giant knotweed, Java brucea, Pogostemon cablin, blackberry lily, wintergreen.
2. a kind of natural bactericidal agent of preventing and treating the peach brown rot according to claim 1, it is characterized in that: the component by following weight portion is made: balsam pear 4-6 part, reed 4-6 part, root of kudzu vine 4-6 part, birthwort 2-4 part, bighead atractylodes rhizome 2-4 part, giant knotweed 1-3 part, Java brucea 1-3 part, Pogostemon cablin 1-3 part, blackberry lily 0.5-1.5 part, wintergreen 0.5-1.5 part.
3. a kind of natural bactericidal agent of preventing and treating the peach brown rot according to claim 1, it is characterized in that: the component by following weight portion is made: 5 parts in balsam pear, 5 parts in reed, 5 parts of the roots of kudzu vine, 3 parts of birthworts, 3 parts of the bighead atractylodes rhizomes, 2 parts of giant knotweeds, 2 parts of Java brucea, 2 parts of Pogostemon cablin, 1 part of blackberry lily, 1 part of wintergreen.
4. preparation method who prevents and treats the natural bactericidal agent of peach brown rot is characterized in that: may further comprise the steps:
(1) with raw material mixing after pulverizing, sieving respectively, gets compound; Described raw material is balsam pear, reed, the root of kudzu vine, birthwort, the bighead atractylodes rhizome, giant knotweed, Java brucea, Pogostemon cablin, blackberry lily and wintergreen;
(2) compound is added 5-10 and doubly measure 95% ethanol water, be heated to 50-70 ℃ and decoct 2-4h;
(3) filter, filtrate is collected, and filter residue adds 5-10 and doubly measures 95% ethanol water, is heated to 50-70 ℃ and decocts 2-4h;
(4) filter, merging filtrate is concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, leaves standstill 24-48h;
(5) filter, filtrate is concentrated into does not have the alcohol flavor, adds with the water-soluble solution of weight, namely gets the natural bactericidal agent of control peach brown rot.
5. the application of the natural bactericidal agent of control peach brown rot in control peach brown rot is characterized in that: the natural bactericidal agent that will prevent and treat the peach brown rot mixes with 500-1000 times of water gaging and is the sterilization microbial inoculum.
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| CN201310216409.2A CN103340218B (en) | 2013-05-31 | 2013-05-31 | Natural bactericide for prevention and treatment of peach brown rot and preparation method thereof |
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| CN201310216409.2A CN103340218B (en) | 2013-05-31 | 2013-05-31 | Natural bactericide for prevention and treatment of peach brown rot and preparation method thereof |
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| CN103340218A true CN103340218A (en) | 2013-10-09 |
| CN103340218B CN103340218B (en) | 2015-02-11 |
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Cited By (6)
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| CN104146015A (en) * | 2014-07-21 | 2014-11-19 | 句容市天王镇戴庄绿鑫畜禽养殖专业合作社 | Natural bactericide for preventing and treating scab of sugar tangerine trees and preparation method of natural bactericide |
| CN105053075A (en) * | 2015-07-20 | 2015-11-18 | 句容市后白镇解氏家庭农场 | Natural bactericide for controlling loquat leaf parch blight disease and preparation method of natural bactericide |
| CN105340641A (en) * | 2015-11-18 | 2016-02-24 | 北京联合大学 | Pesticide applying method using biopesticide to control peach tree insect disease in whole process |
| CN105494456A (en) * | 2015-12-09 | 2016-04-20 | 赵兰 | Pollution-free sterilizing agent capable of preventing and treating trunk blight disease of waxberries and preparation method thereof |
| CN107125273A (en) * | 2017-06-20 | 2017-09-05 | 松桃苗族自治县继梅养殖专业合作社 | A kind of composition for preventing and treating grape grey mould and preparation method thereof |
| CN107347519A (en) * | 2017-07-28 | 2017-11-17 | 贞丰县维康精品水果有限公司 | A kind of method for preventing and treating peach brown rot |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104146015A (en) * | 2014-07-21 | 2014-11-19 | 句容市天王镇戴庄绿鑫畜禽养殖专业合作社 | Natural bactericide for preventing and treating scab of sugar tangerine trees and preparation method of natural bactericide |
| CN105053075A (en) * | 2015-07-20 | 2015-11-18 | 句容市后白镇解氏家庭农场 | Natural bactericide for controlling loquat leaf parch blight disease and preparation method of natural bactericide |
| CN105340641A (en) * | 2015-11-18 | 2016-02-24 | 北京联合大学 | Pesticide applying method using biopesticide to control peach tree insect disease in whole process |
| CN105494456A (en) * | 2015-12-09 | 2016-04-20 | 赵兰 | Pollution-free sterilizing agent capable of preventing and treating trunk blight disease of waxberries and preparation method thereof |
| CN107125273A (en) * | 2017-06-20 | 2017-09-05 | 松桃苗族自治县继梅养殖专业合作社 | A kind of composition for preventing and treating grape grey mould and preparation method thereof |
| CN107347519A (en) * | 2017-07-28 | 2017-11-17 | 贞丰县维康精品水果有限公司 | A kind of method for preventing and treating peach brown rot |
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| CN103340218B (en) | 2015-02-11 |
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