CN107041388A - A kind of natural bactericidal agent for preventing and treating reed wormwood artemisia southern blight and preparation method and application - Google Patents
A kind of natural bactericidal agent for preventing and treating reed wormwood artemisia southern blight and preparation method and application Download PDFInfo
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- CN107041388A CN107041388A CN201710304486.1A CN201710304486A CN107041388A CN 107041388 A CN107041388 A CN 107041388A CN 201710304486 A CN201710304486 A CN 201710304486A CN 107041388 A CN107041388 A CN 107041388A
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- wormwood artemisia
- southern blight
- bactericidal agent
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/34—Rosaceae [Rose family], e.g. strawberry, hawthorn, plum, cherry, peach, apricot or almond
Abstract
A kind of natural bactericidal agent for preventing and treating reed wormwood artemisia southern blight that the present invention is provided, is made up of following component：Purslane, subprostrate sophora, Mu Zeihua, dewdrop grass, white Phytolacca acinosa, luffa vine, the seed of Oriental arborvitae, bittersweet, anise, Rubus parvifolius.The natural bactericidal agent raw material sources of the preventing and treating reed wormwood artemisia southern blight enrich, with low cost, are made of pure natural component, not only Nonpoisonous, non-environmental-pollution, and can effectively prevent and treat reed wormwood artemisia southern blight.
Description
Technical field
The invention belongs to prevent and treat pest and disease damage field, more particularly to a kind of natural bactericidal agent and its system for preventing and treating reed wormwood artemisia southern blight
Preparation Method.
Background technology
Reed wormwood artemisia (scientific name：Artemisia selengensis Turcz.ex Bess.) it is composite family sagebruss.Young stem and leaf,
Root-like stock, also known as artemisia selengensis, water Chinese mugwort, water wormwood artemisia etc..Perennial herb；Plant has faint scent.Main root is not substantially or slightly obvious, has many
Number lateral root and fibrous fibrous root；Very shape stem is slightly thick, uprightly or obliquely, 4-10 millimeters of diameter, there is creeping underground stem.Leaf papery or
Papery, above green, hairless or near hairless, the close wool smooth by canescence spider's thread shape in the back side；The lower portion of the stem leaf width it is avette or
It is avette, totally cleaved or drastic crack closely into palmate or finger-like, 5 or 3, divide leaf sliver is linear or wire lanceolar.Capitulum is most,
Long Circle or width egg shape, and constitute on stem panicle that is narrow and extending.Achene is avette, slightly flat, and upper end occasionally has asymmetric
Corolla areloe.The flowering fruit bearing stage 7-10 months.
Reed wormwood artemisia is the herbaceos perennial of wet middle raw shade tolerance.In general 4 months of north of China, the last ten-days period sprout, and open July
Flower, late August to early September is ripe, and mid-October, plant was withered and yellow.It is born in forest, forest land grassland and flat-bottomed land more.It is common in
Sylvan life, border, gully and river valley two sides, also see Plain limes marginis, pool edge and paddy field ridge side.It is the companion of grassy marshland and Marsh meadow
Non-hibernating eggs.In some areas, such as ditch, paddy field side or pond bank can turn into sociales, or even can form the pure group of single excellent kind
Segment.Sometimes also it is scattered near cottage, roadside, Tian Bian, as common weeds.Reed wormwood artemisia has long-rhizome, forms close in underground
Collect root layer, procreation ability is also very strong on the ground, therefore, often into aggregated pattern in group.Sometimes moist climate region wasteland is turned into
The pionner of succession.
Southern blight is one of common disease of reed wormwood artemisia.Southern blight mainly infects subaerial basal part of stem, can also encroach on fruit.
Basal part of stem or vines near the ground are caught an illness, just in crineous, above have white tiffany shape mycelia, radially, edge is particularly evident, after
Phase bears many dark brown rapeseed shape sclerotium in sick portion, when sick stem rots completely, and cauline leaf is all wilted, and plant is withered soon.
At present, for preventing and treating reed wormwood artemisia disease it is usually used be chemical pesticide bactericide, due to a large amount of using chemical pesticides
The problem of environmental pollution caused is on the rise.Meanwhile, using chemical pesticide bactericide, plant pathogen strain is produced resistance
Property, the resistance to the action of a drug, add the difficulty of preventing and treating, or even cause preventing and treating to fail.In addition, chemical pesticide is because it is without selectivity, it is right
Beneficial biology will also result in threat.Moreover, many chemical pesticides are non-degradables or are difficult to degraded, easily in animal and plant body
Interior accumulation and pollute the enrichment of thing.
With requirement more and more higher of the public to " pollution-free food ", " pollution-free food ", market to it is pollution-free, can drop naturally
The ecological agricultural chemical demand of solution also gradually increases.From the point of view of the development of pesticide science, upper " back to nature " is used in pesticide manufacturing
And " Harmless " of agricultural chemicals, it is the inexorable trend of society and Development of Natural Science, therefore targetedly bactericide has extensively
Wealthy development prospect.
The content of the invention
Goal of the invention：It is an object of the invention to provide the good preventing and treating reed wormwood artemisia southern blight of a kind of environmental protection, bactericidal effect
Natural bactericidal agent.
Technical scheme：A kind of natural bactericidal agent for preventing and treating reed wormwood artemisia southern blight that the present invention is provided, is made up of following component：Horse
Bitterroot, subprostrate sophora, Mu Zeihua, dewdrop grass, white Phytolacca acinosa, luffa vine, the seed of Oriental arborvitae, bittersweet, anise, Rubus parvifolius.
As an improvement, being made up of the component of following parts by weight：32-38 parts of purslane, 20-24 parts of subprostrate sophora, scouring rush spend 14-
18 parts, 8-12 parts of dewdrop grass, white Phytolacca acinosa 2-4 parts, 2-4 parts of luffa vine, 2-4 parts of the seed of Oriental arborvitae, 1-3 parts of bittersweet, 1-3 parts of anise, thatch
1-3 parts of the certain kind of berries.
Improve, be made up of the component of following parts by weight as another：34-35 parts of purslane, 21-22 parts of subprostrate sophora, wood
Thief flower 16-17 parts, 9-10 parts of dewdrop grass, white Phytolacca acinosa 3-4 parts, 2-3 parts of luffa vine, 2-3 parts of the seed of Oriental arborvitae, 2-3 parts of bittersweet, anise 2-
3 parts, 1-2 parts of Rubus parvifolius.
Improve, be made up of the component of following parts by weight as another：34.8 parts of purslane, 21.2 parts of subprostrate sophora, scouring rush
Spend 16.5 parts, 9.7 parts of dewdrop grass, 3.6 parts of white Phytolacca acinosa, 2.7 parts of luffa vine, 2.5 parts of the seed of Oriental arborvitae, 2.6 parts of bittersweet, anistree 2.4 parts,
1.8 parts of Rubus parvifolius.
Present invention also offers the preparation method of the natural bactericidal agent of above-mentioned preventing and treating reed wormwood artemisia southern blight, comprise the following steps：
(1) mixed after raw material being distinguished into size-reduced, sieving, obtain compound；The raw material is purslane, subprostrate sophora, scouring rush
Flower, dewdrop grass, white Phytolacca acinosa, luffa vine, the seed of Oriental arborvitae, bittersweet, anise and Rubus parvifolius；
(2) add mixture into 5-10 times measure 95% ethanol water, be heated to 50-70 DEG C decoction 2-4h；
(3) filter, filtrate is collected, 95% ethanol water is measured in 5-10 times of filter residue addition, be heated to 50-70 DEG C of decoction 2-
4h；
(4) filter, merging filtrate, be concentrated into the 1/20-1/10 that ethanol adds total amount, add 1-3 times of volume of water, stand
24-48h；
(5) filter, filtrate is concentrated into no alcohol taste, add with weight water dissolving, produce the natural of preventing and treating reed wormwood artemisia southern blight and kill
Microbial inoculum.
Present invention also offers the application of the natural bactericidal agent of above-mentioned preventing and treating reed wormwood artemisia southern blight, it is specially：Reed wormwood artemisia will be prevented and treated
The natural bactericidal agent of southern blight is measured water with 500-1000 times and mixed Ji Wei sterilization microbial inoculum.
Beneficial effect：The natural bactericidal agent raw material sources for the preventing and treating reed wormwood artemisia southern blight that the present invention is provided enrich, with low cost,
It is made of pure natural component, not only Nonpoisonous, non-environmental-pollution, and can effectively prevents and treats reed wormwood artemisia southern blight.
Embodiment
According to following embodiments, the present invention may be better understood.However, as it will be easily appreciated by one skilled in the art that real
Apply specific material proportion, process conditions and its result described by example and be merely to illustrate the present invention, without that will not also should limit
The present invention described in detail in claims processed.
Embodiment 1
The natural bactericidal agent of reed wormwood artemisia southern blight is prevented and treated, is made up of the component of following parts by weight：32 parts of purslane, subprostrate sophora 24
Part, scouring rush spend 14 parts, 8 parts of dewdrop grass, 2 parts of white Phytolacca acinosa, 4 parts of luffa vine, 4 parts of the seed of Oriental arborvitae, 1 part of bittersweet, anistree 1 part, Rubus parvifolius 3
Part.
Its preparation method, comprises the following steps：
(1) mixed after raw material being distinguished into size-reduced, sieving, obtain compound；The raw material is purslane, subprostrate sophora, scouring rush
Flower, dewdrop grass, white Phytolacca acinosa, luffa vine, the seed of Oriental arborvitae, bittersweet, anise and Rubus parvifolius；
(2) ethanol water of 8 times of amounts 95% is added mixture into, 60 DEG C of decoction 3h are heated to；
(3) filter, filtrate is collected, filter residue adds the ethanol water of 8 times of amounts 95%, be heated to 60 DEG C of decoction 2-4h；
(4) filter, merging filtrate, be concentrated into ethanol adds total amount 1/15, add the water of 2 times of volumes of concentrate, stand
36h；
(5) filter, filtrate is concentrated into no alcohol taste, add with weight water dissolving, produce the natural of preventing and treating reed wormwood artemisia southern blight and kill
Microbial inoculum.
Embodiment 2
The natural bactericidal agent of reed wormwood artemisia southern blight is prevented and treated, is made up of the component of following parts by weight：38 parts of purslane, subprostrate sophora 20
Part, scouring rush spend 18 parts, 12 parts of dewdrop grass, 4 parts of white Phytolacca acinosa, 2 parts of luffa vine, 2 parts of the seed of Oriental arborvitae, 3 parts of bittersweet, anistree 3 parts, Rubus parvifolius 1
Part.
Its preparation method, comprises the following steps：
(1) mixed after raw material being distinguished into size-reduced, sieving, obtain compound；The raw material is purslane, subprostrate sophora, scouring rush
Flower, dewdrop grass, white Phytolacca acinosa, luffa vine, the seed of Oriental arborvitae, bittersweet, anise and Rubus parvifolius；
(2) ethanol water of 5 times of amounts 95% is added mixture into, 70 DEG C of decoction 4h are heated to；
(3) filter, filtrate is collected, filter residue adds the ethanol water of 5 times of amounts 95%, be heated to 70 DEG C of decoction 4h；
(4) filter, merging filtrate, be concentrated into ethanol adds total amount 1/20, add the water of 1 times of volume of concentrate, stand
48h；
(5) filter, filtrate is concentrated into no alcohol taste, add with weight water dissolving, produce the natural of preventing and treating reed wormwood artemisia southern blight and kill
Microbial inoculum.
Embodiment 3
The natural bactericidal agent of reed wormwood artemisia southern blight is prevented and treated, is made up of the component of following parts by weight：35 parts of purslane, subprostrate sophora 21
Part, scouring rush spend 16 parts, 10 parts of dewdrop grass, 3 parts of white Phytolacca acinosa, 3 parts of luffa vine, 2 parts of the seed of Oriental arborvitae, 3 parts of bittersweet, anistree 2 parts, Rubus parvifolius 2
Part.
Its preparation method, comprises the following steps：
(1) mixed after raw material being distinguished into size-reduced, sieving, obtain compound；The raw material is purslane, subprostrate sophora, scouring rush
Flower, dewdrop grass, white Phytolacca acinosa, luffa vine, the seed of Oriental arborvitae, bittersweet, anise and Rubus parvifolius；
(2) ethanol water of 10 times of amounts 95% is added mixture into, 50 DEG C of decoction 2h are heated to；
(3) filter, filtrate is collected, filter residue adds the ethanol water of 10 times of amounts 95%, be heated to 50 DEG C of decoction 2h；
(4) filter, merging filtrate, be concentrated into ethanol adds total amount 1/10, add the water of 3 times of volumes of concentrate, stand
24h；
(5) filter, filtrate is concentrated into no alcohol taste, add with weight water dissolving, produce the natural of preventing and treating reed wormwood artemisia southern blight and kill
Microbial inoculum.
Embodiment 4
The natural bactericidal agent of reed wormwood artemisia southern blight is prevented and treated, is made up of the component of following parts by weight：34 parts of purslane, subprostrate sophora 22
Part, scouring rush spend 17 parts, 9 parts of dewdrop grass, 4 parts of white Phytolacca acinosa, 2 parts of luffa vine, 3 parts of the seed of Oriental arborvitae, 2 parts of bittersweet, anistree 3 parts, Rubus parvifolius 1
Part.
Its preparation method is same as Example 3.
Embodiment 5
The natural bactericidal agent of reed wormwood artemisia southern blight is prevented and treated, is made up of the component of following parts by weight：34.8 parts of purslane, subprostrate sophora
21.2 parts, scouring rush spend 16.5 parts, 9.7 parts of dewdrop grass, 3.6 parts of white Phytolacca acinosa, 2.7 parts of luffa vine, 2.5 parts of the seed of Oriental arborvitae, bittersweet 2.6
Part, anistree 2.4 parts, 1.8 parts of Rubus parvifolius.
Its preparation method is same as Example 3.
Determine inhibitory activity of the natural bactericidal agent for preventing and treating reed wormwood artemisia southern blight to pathogen of embodiment 1 to 5
Comparative example is the microbial inoculum prepared using the method for embodiment 5, and consumption is identical with component weight in embodiment 5.
This experiment determines the natural bactericidal agent of preventing and treating reed wormwood artemisia southern blight of embodiment 1 to 5 to disease in vitro
The inhibitory activity of opportunistic pathogen, method and result are as follows：
1. for examination strain
Sclerotium rolfsii (Sclerotium rolfsii Saccardo) is commercially available.
2. Toxicity Determination
The natural bactericidal agent of the embodiment of the present invention 1 is determined to sclerotium rolfsii using mycelial growth rate method and spore germination method
The virulence of (Sclerotium rolfsii Saccardo), inhibiting rate is calculated by following equation：
3. experimental result
Embodiment 1-5 natural bactericidal agents are determined to the virulence for trying pathogen with growth rate method, the results are shown in Table 1.
The embodiment 1-5 natural bactericidal agents of table 1 are to the virulence for trying pathogen
Using《Pesticide field efficacy medicine test criterion》Specified in method, to the natural bactericidal agent of the embodiment of the present invention 1 to 5
Field efficacy tested, diluted concentration be 500-1000 times, the results are shown in Table 2.
To for examination pathogen：Sclerotium rolfsii (Sclerotium rolfsii Saccardo).
The field control effectiveness test of the natural bactericidal agent of the embodiment 1 to 5 of table 2
Reed wormwood artemisia southern blight, respectively 100 plants of the treatment, extension rate of various diseases are treated using the natural bactericidal agent of embodiment 1 to 5
For 500 times, 3 are the results are shown in Table.
To for examination pathogen：Sclerotium rolfsii (Sclerotium rolfsii Saccardo).
The natural bactericidal agent treatment reed wormwood artemisia southern blight of the embodiment 1 to 3 of table 3
Claims (5)
1. a kind of natural bactericidal agent for preventing and treating reed wormwood artemisia southern blight, it is characterised in that：It is made up of following component：Purslane, subprostrate sophora,
Scouring rush spends, dewdrop grass, white Phytolacca acinosa, luffa vine, the seed of Oriental arborvitae, bittersweet, anise, Rubus parvifolius.
2. a kind of natural bactericidal agent for preventing and treating reed wormwood artemisia southern blight according to claim 1, it is characterised in that：By following weight
The component of part is made：32-38 parts of purslane, 20-24 parts of subprostrate sophora, scouring rush spend 14-18 parts, 8-12 parts of dewdrop grass, white Phytolacca acinosa 2-4
Part, 2-4 parts of luffa vine, 2-4 parts of the seed of Oriental arborvitae, 1-3 parts of bittersweet, 1-3 parts of anise, 1-3 parts of Rubus parvifolius.
3. a kind of natural bactericidal agent for preventing and treating reed wormwood artemisia southern blight according to claim 1, it is characterised in that：By following weight
The component of part is made：34-35 parts of purslane, 21-22 parts of subprostrate sophora, scouring rush spend 16-17 parts, 9-10 parts of dewdrop grass, white Phytolacca acinosa 3-4
Part, 2-3 parts of luffa vine, 2-3 parts of the seed of Oriental arborvitae, 2-3 parts of bittersweet, 2-3 parts of anise, 1-2 parts of Rubus parvifolius.
4. a kind of natural bactericidal agent for preventing and treating reed wormwood artemisia southern blight according to claim 1, it is characterised in that：By following weight
The component of part is made：34.8 parts of purslane, 21.2 parts of subprostrate sophora, scouring rush spend 16.5 parts, 9.7 parts of dewdrop grass, 3.6 parts of white Phytolacca acinosa,
2.7 parts of luffa vine, 2.5 parts of the seed of Oriental arborvitae, 2.6 parts of bittersweet, anistree 2.4 parts, 1.8 parts of Rubus parvifolius.
5. prevent and treat application of the natural bactericidal agent of reed wormwood artemisia southern blight in preventing and treating reed wormwood artemisia southern blight, it is characterised in that：Reed will be prevented and treated
The natural bactericidal agent of wormwood artemisia southern blight is measured water with 500-1000 times and mixed Ji Wei sterilization microbial inoculum.
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Application publication date: 20170815 |