CN103380787B - Natural bactericide for preventing and curing nursery stock damping-off and preparation method thereof - Google Patents
Natural bactericide for preventing and curing nursery stock damping-off and preparation method thereof Download PDFInfo
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Abstract
The invention provides a natural bactericide for preventing and curing nursery stock damping-off. The natural bactericide is composed of following components: mulberry leaves, tarragon, celandine, acalypha australis, coptis, buddhas lamp twigs and leaves, pogostemon, cirsium japonicum, aconitum kusnezoffii, and cordate houttuynia. The natural bactericide for preventing and curing nursery stock damping-off has the advantages of wide raw material resources and low cost, is composed of pure natural components, not only is non-toxic and non-harmful, but also can effectively prevent and cure the nursery stock damping-off.
Description
Technical field
The invention belongs to prevention and elimination of disease and pests field, particularly a kind of natural bactericidal agent preventing and treating seedling damping-off and preparation method thereof.
Background technology
Seedling damping-off, is commonly called as " seedling ", " mould ", " bound feet pest ", is the most serious the most general disease occurred in Gardening Nursery, mainly betides the Seedling Stage of forest flower plant.Caused by melon and fruit pythium Mastigomycotina fungal infection.Rotten some kinds that are mould and Phytophthora of thorn also can cause morbidity.Germ host range is very wide, can cause dead seedling in flakes time serious.Contaminated and rot when seed or young shoot are not unearthed, falling ill at Seedling Stage, the basal part of stem under earth's surface or earth's surface presents water stain shape scab, sick portion yellowish-brown, contracting of hanging, and can expand to plant top and the bottom, in wire.Patient's condition development rapidly, organizes disintegration, and namely young stem wilts lodging, but in a short time limb in green, if during humid environment, sick portion and near also to grow white wool shape mould in native face.
At present, in vegetables, melon and fruit plantation, be chemical pesticide bactericide for preventing fungi normally used, the problem of environmental pollution caused owing to using chemical pesticide is in a large number on the rise.Meanwhile, adopt chemical pesticide bactericide, time various phytopathogen bacterial classification produce drug resistance, pesticide resistance, add the difficulty of control, even cause and prevent and treat unsuccessfully.In addition, chemical pesticide does not have selectivity because of it, also can threaten to useful biology.And a lot of chemical pesticide is non-degradable or is difficult to degraded, easily pollutes the enrichment of thing at animals and plants cylinder accumulation.
Along with the requirement of the public to " pollution-free food ", " pollution-free food " is more and more higher, market to pollution-free, can the ecological agricultural chemical demand of natural degradation also increase gradually.From the development of pesticide science, " Harmless " of " back to nature " and agricultural chemicals on pesticide manufacturing uses, be the inexorable trend of society and Development of Natural Science, therefore new bio wide-spectrum bactericide has wide development prospect.
Summary of the invention
Goal of the invention: the natural bactericidal agent that the object of this invention is to provide a kind of environmental protection, control seedling damping-off that bactericidal effect is good.
Technical scheme: a kind of natural bactericidal agent preventing and treating seedling damping-off provided by the invention, is made up of following component: mulberry leaf, tarragon, greater celandine, acalypha copperleaf, the coptis, Dong's sauce back of the body, Pogostemon cablin, setose thistle, radix aconiti agrestis, cordate houttuynia.
Be made up of the component of following weight portion: mulberry leaf 4-6 part, tarragon 4-6 part, greater celandine 4-6 part, acalypha copperleaf 4-6 part, coptis 3-5 part, Dong's sauce back of the body 1-3 part, Pogostemon cablin 1-3 part, setose thistle 1-3 part, radix aconiti agrestis 0.5-1.5 part, cordate houttuynia 1-3 part.
Be made up of the component of following weight portion: 5 parts, mulberry leaf, tarragon 5 parts, greater celandine 5 parts, acalypha copperleaf 5 parts, the coptis 4 parts, Dong's sauce carry on the back 2 parts, Pogostemon cablin 2 parts, setose thistle 2 parts, radix aconiti agrestis 1 part, cordate houttuynia 2 parts.
Present invention also offers the preparation method of the natural bactericidal agent of above-mentioned control seedling damping-off, comprise the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is mulberry leaf, tarragon, greater celandine, acalypha copperleaf, the coptis, Dong's sauce back of the body, Pogostemon cablin, setose thistle, radix aconiti agrestis and cordate houttuynia;
(2) compound is added the ethanol water of 5-10 times amount 95%, be heated to 60-80 DEG C and decoct 1-3h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5-10 times amount 95%, is heated to 60-80 DEG C and decocts 1-3h;
(4) filter, merging filtrate, is concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, leaves standstill 24-48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of seedling damping-off.
Present invention also offers the application of natural bactericidal agent in control seedling damping-off of above-mentioned control seedling damping-off, be specially: the natural bactericidal agent of control seedling damping-off is mixed with 500-1000 times of water gaging and is sterilization microbial inoculum.
Beneficial effect: the natural bactericidal agent abundant raw material source of control seedling damping-off provided by the invention, with low cost, adopt the component of pure natural to make, not only Nonpoisonous, non-environmental-pollution, and effectively can prevent and treat seedling damping-off.
Embodiment
According to following embodiment, the present invention may be better understood.But those skilled in the art will readily understand, concrete material proportion, process conditions and result thereof described by embodiment only for illustration of the present invention, and should can not limit the present invention described in detail in claims yet.
Embodiment 1
The natural bactericidal agent of control seedling damping-off, be made up of the component of following weight portion: 4 parts, mulberry leaf, tarragon 5 parts, greater celandine 4 parts, acalypha copperleaf 5 parts, the coptis 4 parts, Dong's sauce carry on the back 1 part, Pogostemon cablin 3 parts, setose thistle 3 parts, radix aconiti agrestis 1.5 parts, cordate houttuynia 2 parts.
Its preparation method, comprises the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is mulberry leaf, tarragon, greater celandine, acalypha copperleaf, the coptis, Dong's sauce back of the body, Pogostemon cablin, setose thistle, radix aconiti agrestis and cordate houttuynia;
(2) compound is added the ethanol water of 8 times amount 95%, be heated to 70 DEG C and decoct 2h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 8 times amount 95%, is heated to 60 DEG C and decocts 1-3h;
(4) filter, merging filtrate, be concentrated into that ethanol adds total amount 1/15, add the water of concentrate 2 times of volumes, leave standstill 36h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of seedling damping-off.
Embodiment 2
The natural bactericidal agent of control seedling damping-off, be made up of the component of following weight portion: 6 parts, mulberry leaf, tarragon 6 parts, greater celandine 6 parts, acalypha copperleaf 4 parts, the coptis 3 parts, Dong's sauce carry on the back 3 parts, Pogostemon cablin 1 part, setose thistle 1 part, radix aconiti agrestis 0.5 part, cordate houttuynia 1 part.
Its preparation method, comprises the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is mulberry leaf, tarragon, greater celandine, acalypha copperleaf, the coptis, Dong's sauce back of the body, Pogostemon cablin, setose thistle, radix aconiti agrestis and cordate houttuynia;
(2) compound is added the ethanol water of 5 times amount 95%, be heated to 80 DEG C and decoct 1h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5 times amount 95%, is heated to 70 DEG C and decocts 4h;
(4) filter, merging filtrate, be concentrated into that ethanol adds total amount 1/20, add the water of concentrate 1 times of volume, leave standstill 48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of seedling damping-off.
Embodiment 3
The natural bactericidal agent of control seedling damping-off, be made up of the component of following weight portion: 5 parts, mulberry leaf, tarragon 4 parts, greater celandine 5 parts, acalypha copperleaf 6 parts, the coptis 5 parts, Dong's sauce carry on the back 2 parts, Pogostemon cablin 2 parts, setose thistle 2 parts, radix aconiti agrestis 1 part, cordate houttuynia 3 parts.
Its preparation method, comprises the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is mulberry leaf, tarragon, greater celandine, acalypha copperleaf, the coptis, Dong's sauce back of the body, Pogostemon cablin, setose thistle, radix aconiti agrestis and cordate houttuynia;
(2) compound is added the ethanol water of 10 times amount 95%, be heated to 60 DEG C and decoct 3h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 10 times amount 95%, is heated to 50 DEG C and decocts 2h;
(4) filter, merging filtrate, be concentrated into that ethanol adds total amount 1/10, add the water of concentrate 3 times of volumes, leave standstill 24h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of seedling damping-off.
Embodiment 4 measures the natural bactericidal agent of the control seedling damping-off of embodiment 1 to 3 to the inhibit activities of strawberry pathogen
This experiment, in vitro, measures the natural bactericidal agent of control seedling damping-off of embodiment 1 to 3 to the inhibit activities of strawberry pathogen, method and result as follows:
1. for examination bacterial classification
Pythium spp (Pythium aphanidermatum (Fds.) Fitz.) is commercially available
Sickle-like bacteria (Fusarium solani Mart App.et.Woll) is commercially available
Rhizoctonia solani Kuhn (Rhizoctonia solani Kuhn) is commercially available
2. Toxicity Determination
Mycelial growth rate method and spore germination method is utilized to measure the embodiment of the present invention 1 natural bactericidal agent to the virulence of above-mentioned germ, by following formulae discovery inhibiting rate:
3. experimental result
Measure embodiment 1 natural bactericidal agent to the virulence for examination pathogen with growth rate method, the results are shown in Table 1.
Table 1 embodiment 1 natural bactericidal agent is to the virulence of pythium spp
Table 2 embodiment 1 natural bactericidal agent is to the virulence of sickle-like bacteria
The virulence of table 3 embodiment 1 natural bactericidal agent Rhizoctonia solani
Adopt the method for regulation in " pesticide field efficacy medicine test criterion ", test the field efficacy of the natural bactericidal agent of the embodiment of the present invention 1 to 3, diluted concentration is 500-1000 times, the results are shown in Table 2.
The field control effectiveness test of the natural bactericidal agent of table 2 embodiment 1 to 3
Adopt the natural bactericidal agent treatment seedling damping-off of embodiment 1 to 3, various disease respectively treats 100 strains, and extension rate is 500 times, the results are shown in Table 3.
The natural bactericidal agent treatment strawberry disease of table 3 embodiment 1 to 3
Claims (4)
1. prevent and treat a natural bactericidal agent for seedling damping-off, it is characterized in that: be made up of the component of following weight portion: mulberry leaf 4-6 part, tarragon 4-6 part, greater celandine 4-6 part, acalypha copperleaf 4-6 part, coptis 3-5 part, Dong's sauce back of the body 1-3 part, Pogostemon cablin 1-3 part, setose thistle 1-3 part, radix aconiti agrestis 0.5-1.5 part, cordate houttuynia 1-3 part;
The preparation method of this bactericide, comprises the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is mulberry leaf, tarragon, greater celandine, acalypha copperleaf, the coptis, Dong's sauce back of the body, Pogostemon cablin, setose thistle, radix aconiti agrestis and cordate houttuynia;
(2) compound is added the ethanol water of 5-10 times amount 95%, be heated to 60-80 DEG C and decoct 1-3h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5-10 times amount 95%, is heated to 60-80 DEG C and decocts 1-3h;
(4) filter, merging filtrate, is concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, leaves standstill 24-48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of seedling damping-off.
2. a kind of natural bactericidal agent preventing and treating seedling damping-off according to claim 1, is characterized in that: be made up of the component of following weight portion: 5 parts, mulberry leaf, tarragon 5 parts, greater celandine 5 parts, acalypha copperleaf 5 parts, the coptis 4 parts, Dong's sauce carry on the back 2 parts, Pogostemon cablin 2 parts, setose thistle 2 parts, radix aconiti agrestis 1 part, cordate houttuynia 2 parts.
3. a preparation method for the natural bactericidal agent of the control seedling damping-off described in claim 1 or 2, is characterized in that: comprise the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is mulberry leaf, tarragon, greater celandine, acalypha copperleaf, the coptis, Dong's sauce back of the body, Pogostemon cablin, setose thistle, radix aconiti agrestis and cordate houttuynia;
(2) compound is added the ethanol water of 5-10 times amount 95%, be heated to 60-80 DEG C and decoct 1-3h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5-10 times amount 95%, is heated to 60-80 DEG C and decocts 1-3h;
(4) filter, merging filtrate, is concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, leaves standstill 24-48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of seedling damping-off.
4. the application of natural bactericidal agent in control seedling damping-off of the control seedling damping-off described in claim 1 or 2, is characterized in that: mixed with 500-1000 times of water gaging by the natural bactericidal agent of control seedling damping-off and be sterilization microbial inoculum.
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105309499A (en) * | 2015-11-18 | 2016-02-10 | 无锡市稼宝药业有限公司 | Botanical fungicide for damping-off disease |
| CN105532768A (en) * | 2016-02-04 | 2016-05-04 | 史运江 | Biopesticide for controlling cabbage bacterial black spot and preparation method |
| CN107027816A (en) * | 2017-05-03 | 2017-08-11 | 沈荣存 | A kind of natural bactericidal agent for preventing and treating safron rust and its application |
| CN106993636A (en) * | 2017-05-03 | 2017-08-01 | 沈荣存 | A kind of preparation method for the natural bactericidal agent for preventing and treating safron rust |
| CN107372666A (en) * | 2017-08-28 | 2017-11-24 | 锦屏县新化乡新化所蔬菜专业合作社 | A kind of composition for preventing and treating vegetable seedling samping off and preparation method thereof |
| CN108064892A (en) * | 2017-11-29 | 2018-05-25 | 沿河土家族自治县双强牧业有限责任公司 | A kind of composition for treating tomato seedling samping off and preparation method thereof |
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