CN105379781A - Natural bactericide for preventing and treating Anoectochilus roxburghii southern blight and preparation method of natural bactericide - Google Patents
Natural bactericide for preventing and treating Anoectochilus roxburghii southern blight and preparation method of natural bactericide Download PDFInfo
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- CN105379781A CN105379781A CN201510762174.6A CN201510762174A CN105379781A CN 105379781 A CN105379781 A CN 105379781A CN 201510762174 A CN201510762174 A CN 201510762174A CN 105379781 A CN105379781 A CN 105379781A
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Abstract
The invention provides a natural bactericide for preventing and treating Anoectochilus roxburghii southern blight. The natural bactericide is prepared from the following components: perilla leaves, manyleaf paris rhizomes, dianthus superbus, lilac daphne flower buds, largehead atractylodes rhizomes, safflowers, motherwort herb, Pogostemon cablin, turmeric and giant knotweed rhizomes. The natural bactericide for preventing and treating the Anoectochilus roxburghii southern blight is abundant in sources of raw materials and low in cost, and is made of the purely natural components so that the natural bactericide has no toxin or pollution and can be used for effectively preventing and treating the Anoectochilus roxburghii southern blight.
Description
Technical field
The invention belongs to prevention and elimination of disease and pests field, particularly a kind ofly prevent and treat natural bactericidal agent of Shorthairy Antenoron southern blight and preparation method thereof.
Background technology
Shorthairy Antenoron (formal name used at school: Anoectochilusroxburghii (Wall.) Lindl.): plant height 8-18 centimetre.Root-like stock is crawled, and extends, meat, and tool saves, and joint is taken root.Stem is upright, and meat is cylindrical, tool 2-4 piece leaf.Blade oval or avette, above mulberry or black purple, tool golden red with the beautiful net arteries and veins of silky lustre, back side lilac red.Raceme tool 2-6 flower, long 3-5 centimetre; Floral white or pale red, be not inverted (lip is positioned at top); The sepal back side is by pubescence, and middle sepal is avette, and depression is in boat shape; Petal quality is thin, nearly sickle shaped.The florescence 8-11 month.Shorthairy Antenoron is born in evergreen broad-leaved sylvan life or the dark and damp place of cheuch of height above sea level 50-1600 rice.Originate in China, also there is distribution in Japan, Thailand, Laos, Vietnam, India, Bhutan to Nepal, Bangladesh.
Roxburgh anoectochilus terminal bud is the orchid family Anoectochilus Blume (gold thread Nelumbo) Anoectochilus plant, is a kind of famous and precious rare Chinese herbal medicine, has refrigerant annealing, the solid lung of cool blood, and wound of dispelling removing toxic substances, opens middle gas, effect of strengthening by means of tonics.But because its reproduction rate is low, poor growth, adds that people surge to roxburgh anoectochilus terminal bud demand in recent years, causes excessively excavating, make the wild resource of roxburgh anoectochilus terminal bud be seriously damaged.Taiwan is own lists one of rare plant of 29 kinds of needs protections in by roxburgh anoectochilus terminal bud, Fujian Province is also classified as provincial natural crude drugs protection kind.The nutritive value had due to roxburgh anoectochilus terminal bud and medical value, commercially supply falls short of demand, and current roxburgh anoectochilus terminal bud is mainly through artificial cultivation, and artificial cultivation pattern mainly contains high standard demonstration greenhouse cultivation and cultivation under woods.Because roxburgh anoectochilus terminal bud growth conditions is unique, easily cause serious damage by disease and insect in cultivation, affect yield and quality.Wherein white beautiful disease is one of comparatively common disease of roxburgh anoectochilus terminal bud, southern blight also known as white mould, the basal part of stem of this sick main harm plant near ground and root.
Southern blight is infected by Roche pyrenomycetes Sclero-tiumrolfsiiSacc. to cause, and is one of orchid worldwide disease, this disease many plum rain season at the end of spring and the beginning of summer and autumn rain unbroken time occur, the root of main harm plant and basal part of stem.White beautiful disease causes harm the research of medicinal plant, what bibliographical information was more is bighead atractylodes rhizome southern blight, Xiao Zhong waits so long and carries out Molecular Identification to bighead atractylodes rhizome southern blight pathogen, adopt 6 kinds of medium to cultivate separate sources bighead atractylodes rhizome southern blight pathogen respectively, and study the Virulence Difference of bactericide to different growing area bighead atractylodes rhizome sclerotium rolfsii.
At present, in the plantings such as vegetables, melon and fruit, flowers, be chemical pesticide bactericide for preventing fungi normally used, the problem of environmental pollution caused owing to using chemical pesticide is in a large number on the rise.Meanwhile, adopt chemical pesticide bactericide, time various phytopathogen bacterial classification produce drug resistance, pesticide resistance, add the difficulty of control, even cause and prevent and treat unsuccessfully.In addition, chemical pesticide does not have selectivity because of it, also can threaten to useful biology.And a lot of chemical pesticide is non-degradable or is difficult to degraded, easily pollutes the enrichment of thing at animals and plants cylinder accumulation.
Along with the requirement of the public to " pollution-free food ", " pollution-free food " is more and more higher, market to pollution-free, can the ecological agricultural chemical demand of natural degradation also increase gradually.From the development of pesticide science, " Harmless " of " back to nature " and agricultural chemicals on pesticide manufacturing uses, be the inexorable trend of society and Development of Natural Science, therefore new bio wide-spectrum bactericide has wide development prospect.
Summary of the invention
Goal of the invention: the natural bactericidal agent that the object of this invention is to provide a kind of environmental protection, control Shorthairy Antenoron southern blight that bactericidal effect is good.
Technical scheme: a kind of natural bactericidal agent preventing and treating Shorthairy Antenoron southern blight provided by the invention, be made up of following component: perilla leaf, flea stop, fringed pink, lilac daphne, the bighead atractylodes rhizome, safflower, siberian motherwort, Pogostemon cablin, turmeric, giant knotweed.
Be made up of the component of following weight portion: perilla leaf 4-6 part, flea stop 4-6 part, fringed pink 4-6 part, lilac daphne 2-4 part, bighead atractylodes rhizome 2-4 part, safflower 1-3 part, siberian motherwort 1-3 part, Pogostemon cablin 1-3 part, turmeric 0.5-1.5 part, giant knotweed 0.5-1.5 part.
Be made up of the component of following weight portion: perilla leaf 5 parts, flea stop 5 parts, fringed pink 5 parts, lilac daphne 3 parts, the bighead atractylodes rhizome 3 parts, 2 parts, safflower, siberian motherwort 2 parts, Pogostemon cablin 2 parts, 1 part, turmeric, giant knotweed 1 part.
Present invention also offers the preparation method of the natural bactericidal agent of above-mentioned control Shorthairy Antenoron southern blight, comprise the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is perilla leaf, flea stops, fringed pink, lilac daphne, the bighead atractylodes rhizome, safflower, siberian motherwort, Pogostemon cablin, turmeric and giant knotweed;
(2) compound is added the ethanol water of 5-10 times amount 60%, be heated to 50-70 DEG C and decoct 2-4h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5-10 times amount 60%, is heated to 50-70 DEG C and decocts 2-4h;
(4) filter, merging filtrate, is concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, leaves standstill 24-48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of Shorthairy Antenoron southern blight.
Present invention also offers the application of the natural bactericidal agent of above-mentioned control Shorthairy Antenoron southern blight, be specially: the natural bactericidal agent of control Shorthairy Antenoron southern blight is mixed with 500-1000 times of water gaging and is sterilization microbial inoculum.
Beneficial effect: the natural bactericidal agent abundant raw material source of control Shorthairy Antenoron southern blight provided by the invention, with low cost, adopt the component of pure natural to make, not only Nonpoisonous, non-environmental-pollution, and can effectively prevent and treat Shorthairy Antenoron southern blight.
Embodiment
According to following embodiment, the present invention may be better understood.But those skilled in the art will readily understand, concrete material proportion, process conditions and result thereof described by embodiment only for illustration of the present invention, and should can not limit the present invention described in detail in claims yet.
Embodiment 1
The natural bactericidal agent of control Shorthairy Antenoron southern blight, be made up of the component of following weight portion: perilla leaf 4 parts, flea stop 6 parts, fringed pink 4 parts, lilac daphne 2 parts, the bighead atractylodes rhizome 2 parts, 1 part, safflower, siberian motherwort 3 parts, Pogostemon cablin 3 parts, 1.5 parts, turmeric, giant knotweed 0.5 part.
Its preparation method, comprises the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is perilla leaf, flea stops, fringed pink, lilac daphne, the bighead atractylodes rhizome, safflower, siberian motherwort, Pogostemon cablin, turmeric and giant knotweed;
(2) compound is added the ethanol water of 8 times amount 60%, be heated to 60 DEG C and decoct 3h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 8 times amount 60%, is heated to 60 DEG C and decocts 2-4h;
(4) filter, merging filtrate, be concentrated into that ethanol adds total amount 1/15, add the water of concentrate 2 times of volumes, leave standstill 36h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of Shorthairy Antenoron southern blight.
Embodiment 2
The natural bactericidal agent of control Shorthairy Antenoron southern blight, be made up of the component of following weight portion: perilla leaf 6 parts, flea stop 4 parts, fringed pink 6 parts, lilac daphne 4 parts, the bighead atractylodes rhizome 4 parts, 3 parts, safflower, siberian motherwort 1 part, Pogostemon cablin 1 part, 0.5 part, turmeric, giant knotweed 1.5 parts.
Its preparation method, comprises the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is perilla leaf, flea stops, fringed pink, lilac daphne, the bighead atractylodes rhizome, safflower, siberian motherwort, Pogostemon cablin, turmeric and giant knotweed;
(2) compound is added the ethanol water of 5 times amount 60%, be heated to 70 DEG C and decoct 4h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5 times amount 60%, is heated to 70 DEG C and decocts 4h;
(4) filter, merging filtrate, be concentrated into that ethanol adds total amount 1/20, add the water of concentrate 1 times of volume, leave standstill 48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of Shorthairy Antenoron southern blight.
Embodiment 3
The natural bactericidal agent of control Shorthairy Antenoron southern blight, be made up of the component of following weight portion: perilla leaf 5 parts, flea stop 5 parts, fringed pink 5 parts, lilac daphne 3 parts, the bighead atractylodes rhizome 3 parts, 2 parts, safflower, siberian motherwort 2 parts, Pogostemon cablin 2 parts, 1 part, turmeric, giant knotweed 1 part.
Its preparation method, comprises the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is perilla leaf, flea stops, fringed pink, lilac daphne, the bighead atractylodes rhizome, safflower, siberian motherwort, Pogostemon cablin, turmeric and giant knotweed;
(2) compound is added the ethanol water of 10 times amount 60%, be heated to 50 DEG C and decoct 2h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 10 times amount 60%, is heated to 50 DEG C and decocts 2h;
(4) filter, merging filtrate, be concentrated into that ethanol adds total amount 1/10, add the water of concentrate 3 times of volumes, leave standstill 24h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of Shorthairy Antenoron southern blight.
Embodiment 4 measures the natural bactericidal agent of the control Shorthairy Antenoron southern blight of embodiment 1 to 3 to the inhibit activities of Shorthairy Antenoron pathogen
This experiment, in vitro, measures the natural bactericidal agent of control Shorthairy Antenoron southern blight of embodiment 1 to 3 to the inhibit activities of Shorthairy Antenoron pathogen, method and result as follows:
1. for examination bacterial classification
Roche pyrenomycetes (Sclero-tiumrolfsiiSacc.) is commercially available.
2. Toxicity Determination
Mycelial growth rate method and spore germination method is utilized to measure the embodiment of the present invention 1 natural bactericidal agent to the virulence of Roche pyrenomycetes (Sclero-tiumrolfsiiSacc.), by following formulae discovery inhibiting rate:
3. experimental result
Measure embodiment 1-3 natural bactericidal agent to the virulence for examination pathogen with growth rate method, the results are shown in Table 1.
Table 1 embodiment 1-3 natural bactericidal agent is to the virulence for examination pathogen
Adopt the method for regulation in " pesticide field efficacy medicine test criterion ", test the field efficacy of the natural bactericidal agent of the embodiment of the present invention 1 to 3, diluted concentration is 500-1000 times, the results are shown in Table 2.
The field control effectiveness test of the natural bactericidal agent of table 2 embodiment 1 to 3
Adopt the natural bactericidal agent treatment Shorthairy Antenoron southern blight of embodiment 1 to 3, various disease respectively treats 100 strains, and extension rate is 500 times, the results are shown in Table 3.
The natural bactericidal agent treatment Shorthairy Antenoron southern blight of table 3 embodiment 1 to 3
Claims (5)
1. prevent and treat a natural bactericidal agent for Shorthairy Antenoron southern blight, it is characterized in that: be made up of following component: perilla leaf, flea stop, fringed pink, lilac daphne, the bighead atractylodes rhizome, safflower, siberian motherwort, Pogostemon cablin, turmeric, giant knotweed.
2. a kind of natural bactericidal agent preventing and treating Shorthairy Antenoron southern blight according to claim 1, is characterized in that: be made up of the component of following weight portion: perilla leaf 4-6 part, flea stop 4-6 part, fringed pink 4-6 part, lilac daphne 2-4 part, bighead atractylodes rhizome 2-4 part, safflower 1-3 part, siberian motherwort 1-3 part, Pogostemon cablin 1-3 part, turmeric 0.5-1.5 part, giant knotweed 0.5-1.5 part.
3. a kind of natural bactericidal agent preventing and treating Shorthairy Antenoron southern blight according to claim 1, is characterized in that: be made up of the component of following weight portion: perilla leaf 5 parts, flea stop 5 parts, fringed pink 5 parts, lilac daphne 3 parts, the bighead atractylodes rhizome 3 parts, 2 parts, safflower, siberian motherwort 2 parts, Pogostemon cablin 2 parts, 1 part, turmeric, giant knotweed 1 part.
4. prevent and treat a preparation method for the natural bactericidal agent of Shorthairy Antenoron southern blight, it is characterized in that: comprise the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is perilla leaf, flea stops, fringed pink, lilac daphne, the bighead atractylodes rhizome, safflower, siberian motherwort, Pogostemon cablin, turmeric and giant knotweed;
(2) compound is added the ethanol water of 5-10 times amount 60%, be heated to 50-70 DEG C and decoct 2-4h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5-10 times amount 60%, is heated to 50-70 DEG C and decocts 2-4h;
(4) filter, merging filtrate, is concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, leaves standstill 24-48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of Shorthairy Antenoron southern blight.
5. prevent and treat the application of natural bactericidal agent in control Shorthairy Antenoron southern blight of Shorthairy Antenoron southern blight, it is characterized in that: the natural bactericidal agent of control Shorthairy Antenoron southern blight is mixed with 500-1000 times of water gaging and is sterilization microbial inoculum.
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| CN105580854A (en) * | 2016-03-11 | 2016-05-18 | 史运江 | Biopesticide for preventing and treating peanut leaf blight and preparation method |
| CN106490047A (en) * | 2016-10-31 | 2017-03-15 | 郑州思辩科技有限公司 | A kind of tea tree specific complex agricultural chemicals and preparation method thereof |
| CN107041388A (en) * | 2017-05-03 | 2017-08-15 | 沈荣存 | A kind of natural bactericidal agent for preventing and treating reed wormwood artemisia southern blight and preparation method and application |
| CN107079938A (en) * | 2017-05-03 | 2017-08-22 | 沈荣存 | A kind of preparation method for the natural bactericidal agent for preventing and treating reed wormwood artemisia southern blight |
| CN107751264A (en) * | 2017-09-15 | 2018-03-06 | 海门市利美农副产品有限公司 | A kind of Farm chemical of Chinese berbs and preparation method thereof |
| CN112741119A (en) * | 2020-12-28 | 2021-05-04 | 浙江农林大学 | Natural southern blight bactericide based on cinnamomum camphora leaves and preparation method thereof |
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2015
- 2015-11-10 CN CN201510762174.6A patent/CN105379781A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105580854A (en) * | 2016-03-11 | 2016-05-18 | 史运江 | Biopesticide for preventing and treating peanut leaf blight and preparation method |
| CN106490047A (en) * | 2016-10-31 | 2017-03-15 | 郑州思辩科技有限公司 | A kind of tea tree specific complex agricultural chemicals and preparation method thereof |
| CN107041388A (en) * | 2017-05-03 | 2017-08-15 | 沈荣存 | A kind of natural bactericidal agent for preventing and treating reed wormwood artemisia southern blight and preparation method and application |
| CN107079938A (en) * | 2017-05-03 | 2017-08-22 | 沈荣存 | A kind of preparation method for the natural bactericidal agent for preventing and treating reed wormwood artemisia southern blight |
| CN107751264A (en) * | 2017-09-15 | 2018-03-06 | 海门市利美农副产品有限公司 | A kind of Farm chemical of Chinese berbs and preparation method thereof |
| CN112741119A (en) * | 2020-12-28 | 2021-05-04 | 浙江农林大学 | Natural southern blight bactericide based on cinnamomum camphora leaves and preparation method thereof |
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Application publication date: 20160309 |