CN105394105A - Natural sterilization agent capable of preventing and treating shorthairy antenoron anthracnose and preparation method of natural sterilization agent - Google Patents

Natural sterilization agent capable of preventing and treating shorthairy antenoron anthracnose and preparation method of natural sterilization agent Download PDF

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Publication number
CN105394105A
CN105394105A CN201510762175.0A CN201510762175A CN105394105A CN 105394105 A CN105394105 A CN 105394105A CN 201510762175 A CN201510762175 A CN 201510762175A CN 105394105 A CN105394105 A CN 105394105A
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CN
China
Prior art keywords
anthracnose
parts
shorthairy antenoron
natural
bactericidal agent
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Pending
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CN201510762175.0A
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Chinese (zh)
Inventor
董长良
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Agricultural Chemicals Co Ltd Of Toyota Of Gaoyou City
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Agricultural Chemicals Co Ltd Of Toyota Of Gaoyou City
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Priority to CN201510762175.0A priority Critical patent/CN105394105A/en
Publication of CN105394105A publication Critical patent/CN105394105A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/40Liliopsida [monocotyledons]
    • A01N65/44Poaceae or Gramineae [Grass family], e.g. bamboo, lemon grass or citronella grass

Abstract

The invention provides a natural sterilization agent capable of preventing and treating shorthairy antenoron anthracnose. The natural sterilization agent is prepared from the following components: India madder roots, common cnidium fruits, talcum, bamboo shavings, antifeverile dichroa roots, Chinese pulsatilla roots, Siberian cocklour fruits, Sinkiang arnebia roots, natural indigo and European verbena. The natural sterilization agent capable of preventing and treating the shorthairy antenoron anthracnose has abundant raw material sources and is low in cost; and the natural sterilization agent is prepared from purely natural components, so that the natural sterilization agent has no toxin and pollution, and can be used for effectively preventing and treating the shorthairy antenoron anthracnose.

Description

A kind ofly prevent and treat natural bactericidal agent of Shorthairy Antenoron anthracnose and preparation method thereof
Technical field
The invention belongs to prevention and elimination of disease and pests field, particularly a kind ofly prevent and treat natural bactericidal agent of Shorthairy Antenoron anthracnose and preparation method thereof.
Background technology
Shorthairy Antenoron (formal name used at school: Anoectochilusroxburghii (Wall.) Lindl.): plant height 8-18 centimetre.Root-like stock is crawled, and extends, meat, and tool saves, and joint is taken root.Stem is upright, and meat is cylindrical, tool 2-4 piece leaf.Blade oval or avette, above mulberry or black purple, tool golden red with the beautiful net arteries and veins of silky lustre, back side lilac red.Raceme tool 2-6 flower, long 3-5 centimetre; Floral white or pale red, be not inverted (lip is positioned at top); The sepal back side is by pubescence, and middle sepal is avette, and depression is in boat shape; Petal quality is thin, nearly sickle shaped.The florescence 8-11 month.Shorthairy Antenoron is born in evergreen broad-leaved sylvan life or the dark and damp place of cheuch of height above sea level 50-1600 rice.Originate in China, also there is distribution in Japan, Thailand, Laos, Vietnam, India, Bhutan to Nepal, Bangladesh.
Roxburgh anoectochilus terminal bud is the orchid family Anoectochilus Blume (gold thread Nelumbo) Anoectochilus plant, is a kind of famous and precious rare Chinese herbal medicine, has refrigerant annealing, the solid lung of cool blood, and wound of dispelling removing toxic substances, opens middle gas, effect of strengthening by means of tonics.But because its reproduction rate is low, poor growth, adds that people surge to roxburgh anoectochilus terminal bud demand in recent years, causes excessively excavating, make the wild resource of roxburgh anoectochilus terminal bud be seriously damaged.Taiwan is own lists one of rare plant of 29 kinds of needs protections in by roxburgh anoectochilus terminal bud, Fujian Province is also classified as provincial natural crude drugs protection kind.The nutritive value had due to roxburgh anoectochilus terminal bud and medical value, commercially supply falls short of demand, and current roxburgh anoectochilus terminal bud is mainly through artificial cultivation, and artificial cultivation pattern mainly contains high standard demonstration greenhouse cultivation and cultivation under woods.Because roxburgh anoectochilus terminal bud growth conditions is unique, easily cause serious damage by disease and insect in cultivation, affect yield and quality.Wherein white beautiful disease is one of comparatively common disease of roxburgh anoectochilus terminal bud, anthracnose also known as white mould, the basal part of stem of this sick main harm plant near ground and root.
Shorthairy Antenoron anthracnose has all the year round, and high temperature and rainy season is more ferocious sticks up.Scab first extends from blade tip to rhizome, is just brown, then expands gradually and increases, occur many dry stains, cause whole strain dead time serious.Prevention and controls: except actively changing Bodhisattva's environmental condition, in period of disease, can first control with the 800-1500 times of liquid spray of 50% thiophanate methyl wetting powder, every 7-10 days are once; And then be aided with 1% equivalent formula bordeaux mixture, every first quarter moon once, continuous spraying 3-5 time.
At present, in the plantings such as vegetables, melon and fruit, flowers, be chemical pesticide bactericide for preventing fungi normally used, the problem of environmental pollution caused owing to using chemical pesticide is in a large number on the rise.Meanwhile, adopt chemical pesticide bactericide, time various phytopathogen bacterial classification produce drug resistance, pesticide resistance, add the difficulty of control, even cause and prevent and treat unsuccessfully.In addition, chemical pesticide does not have selectivity because of it, also can threaten to useful biology.And a lot of chemical pesticide is non-degradable or is difficult to degraded, easily pollutes the enrichment of thing at animals and plants cylinder accumulation.
Along with the requirement of the public to " pollution-free food ", " pollution-free food " is more and more higher, market to pollution-free, can the ecological agricultural chemical demand of natural degradation also increase gradually.From the development of pesticide science, " Harmless " of " back to nature " and agricultural chemicals on pesticide manufacturing uses, be the inexorable trend of society and Development of Natural Science, therefore new bio wide-spectrum bactericide has wide development prospect.
Summary of the invention
Goal of the invention: the natural bactericidal agent that the object of this invention is to provide a kind of environmental protection, control Shorthairy Antenoron anthracnose that bactericidal effect is good.
Technical scheme: a kind of natural bactericidal agent preventing and treating Shorthairy Antenoron anthracnose provided by the invention, is made up of following component: madder, frutus cnidii, talcum, caulis bambusae in taenian, Changshan, the Chinese pulsatilla, the achene of Siberian cocklebur, alkanet, indigo naturalis, Verbena officinalis.
Be made up of the component of following weight portion: madder 8-12 part, frutus cnidii 4-6 part, talcum 4-6 part, caulis bambusae in taenian 2-4 part, Changshan 2-4 part, Chinese pulsatilla 2-4 part, achene of Siberian cocklebur 1-3 part, alkanet 1-3 part, indigo naturalis 0.5-1.5 part, Verbena officinalis 0.5-1.5 part.
Be made up of the component of following weight portion: 10 parts, madder, frutus cnidii 5 parts, 5 parts, talcum, caulis bambusae in taenian 3 parts, Changshan 3 parts, the Chinese pulsatilla 3 parts, the achene of Siberian cocklebur 2 parts, alkanet 2 parts, indigo naturalis 1 part, Verbena officinalis 1 part.
Present invention also offers the preparation method of the natural bactericidal agent of above-mentioned control Shorthairy Antenoron anthracnose, comprise the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is madder, frutus cnidii, talcum, caulis bambusae in taenian, Changshan, the Chinese pulsatilla, the achene of Siberian cocklebur, alkanet, indigo naturalis and Verbena officinalis;
(2) compound is added the ethanol water of 5-10 times amount 60%, be heated to 50-70 DEG C and decoct 2-4h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5-10 times amount 60%, is heated to 50-70 DEG C and decocts 2-4h;
(4) filter, merging filtrate, is concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, leaves standstill 24-48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of Shorthairy Antenoron anthracnose.
Present invention also offers the application of the natural bactericidal agent of above-mentioned control Shorthairy Antenoron anthracnose, be specially: the natural bactericidal agent of control Shorthairy Antenoron anthracnose is mixed with 500-1000 times of water gaging and is sterilization microbial inoculum.
Beneficial effect: the natural bactericidal agent abundant raw material source of control Shorthairy Antenoron anthracnose provided by the invention, with low cost, adopt the component of pure natural to make, not only Nonpoisonous, non-environmental-pollution, and can effectively prevent and treat Shorthairy Antenoron anthracnose.
Embodiment
According to following embodiment, the present invention may be better understood.But those skilled in the art will readily understand, concrete material proportion, process conditions and result thereof described by embodiment only for illustration of the present invention, and should can not limit the present invention described in detail in claims yet.
Embodiment 1
The natural bactericidal agent of control Shorthairy Antenoron anthracnose, is made up of the component of following weight portion: 8 parts, madder, frutus cnidii 6 parts, 4 parts, talcum, caulis bambusae in taenian 2 parts, Changshan 2 parts, the Chinese pulsatilla 2 parts, the achene of Siberian cocklebur 3 parts, alkanet 3 parts, indigo naturalis 1.5 parts, Verbena officinalis 0.5 part.
Its preparation method, comprises the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is madder, frutus cnidii, talcum, caulis bambusae in taenian, Changshan, the Chinese pulsatilla, the achene of Siberian cocklebur, alkanet, indigo naturalis and Verbena officinalis;
(2) compound is added the ethanol water of 8 times amount 60%, be heated to 60 DEG C and decoct 3h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 8 times amount 60%, is heated to 60 DEG C and decocts 2-4h;
(4) filter, merging filtrate, be concentrated into that ethanol adds total amount 1/15, add the water of concentrate 2 times of volumes, leave standstill 36h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of Shorthairy Antenoron anthracnose.
Embodiment 2
The natural bactericidal agent of control Shorthairy Antenoron anthracnose, is made up of the component of following weight portion: 12 parts, madder, frutus cnidii 4 parts, 6 parts, talcum, caulis bambusae in taenian 4 parts, Changshan 4 parts, the Chinese pulsatilla 4 parts, the achene of Siberian cocklebur 1 part, alkanet 1 part, indigo naturalis 0.5 part, Verbena officinalis 1.5 parts.
Its preparation method, comprises the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is madder, frutus cnidii, talcum, caulis bambusae in taenian, Changshan, the Chinese pulsatilla, the achene of Siberian cocklebur, alkanet, indigo naturalis and Verbena officinalis;
(2) compound is added the ethanol water of 5 times amount 60%, be heated to 70 DEG C and decoct 4h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5 times amount 60%, is heated to 70 DEG C and decocts 4h;
(4) filter, merging filtrate, be concentrated into that ethanol adds total amount 1/20, add the water of concentrate 1 times of volume, leave standstill 48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of Shorthairy Antenoron anthracnose.
Embodiment 3
The natural bactericidal agent of control Shorthairy Antenoron anthracnose, is made up of the component of following weight portion: 10 parts, madder, frutus cnidii 5 parts, 5 parts, talcum, caulis bambusae in taenian 3 parts, Changshan 3 parts, the Chinese pulsatilla 3 parts, the achene of Siberian cocklebur 2 parts, alkanet 2 parts, indigo naturalis 1 part, Verbena officinalis 1 part.
Its preparation method, comprises the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is madder, frutus cnidii, talcum, caulis bambusae in taenian, Changshan, the Chinese pulsatilla, the achene of Siberian cocklebur, alkanet, indigo naturalis and Verbena officinalis;
(2) compound is added the ethanol water of 10 times amount 60%, be heated to 50 DEG C and decoct 2h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 10 times amount 60%, is heated to 50 DEG C and decocts 2h;
(4) filter, merging filtrate, be concentrated into that ethanol adds total amount 1/10, add the water of concentrate 3 times of volumes, leave standstill 24h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of Shorthairy Antenoron anthracnose.
Embodiment 4 measures the natural bactericidal agent of the control Shorthairy Antenoron anthracnose of embodiment 1 to 3 to the inhibit activities of Shorthairy Antenoron pathogen
This experiment, in vitro, measures the natural bactericidal agent of control Shorthairy Antenoron anthracnose of embodiment 1 to 3 to the inhibit activities of Shorthairy Antenoron pathogen, method and result as follows:
1. for examination bacterial classification
C. dematium Colletotrichumdematium (Pers.) Grove is commercially available.
2. Toxicity Determination
Mycelial growth rate method and spore germination method is utilized to measure the embodiment of the present invention 1 natural bactericidal agent to the virulence of Roche pyrenomycetes (Sclero-tiumrolfsiiSacc.), by following formulae discovery inhibiting rate:
3. experimental result
Measure embodiment 1-3 natural bactericidal agent to the virulence for examination pathogen with growth rate method, the results are shown in Table 1.
Table 1 embodiment 1-3 natural bactericidal agent is to the virulence for examination pathogen
Adopt the method for regulation in " pesticide field efficacy medicine test criterion ", test the field efficacy of the natural bactericidal agent of the embodiment of the present invention 1 to 3, diluted concentration is 500-1000 times, the results are shown in Table 2.
The field control effectiveness test of the natural bactericidal agent of table 2 embodiment 1 to 3
Adopt the natural bactericidal agent treatment Shorthairy Antenoron anthracnose of embodiment 1 to 3, various disease respectively treats 100 strains, and extension rate is 500 times, the results are shown in Table 3.
The natural bactericidal agent treatment Shorthairy Antenoron anthracnose of table 3 embodiment 1 to 3

Claims (5)

1. prevent and treat a natural bactericidal agent for Shorthairy Antenoron anthracnose, it is characterized in that: be made up of following component: madder, frutus cnidii, talcum, caulis bambusae in taenian, Changshan, the Chinese pulsatilla, the achene of Siberian cocklebur, alkanet, indigo naturalis, Verbena officinalis.
2. a kind of natural bactericidal agent preventing and treating Shorthairy Antenoron anthracnose according to claim 1, is characterized in that: be made up of the component of following weight portion: madder 8-12 part, frutus cnidii 4-6 part, talcum 4-6 part, caulis bambusae in taenian 2-4 part, Changshan 2-4 part, Chinese pulsatilla 2-4 part, achene of Siberian cocklebur 1-3 part, alkanet 1-3 part, indigo naturalis 0.5-1.5 part, Verbena officinalis 0.5-1.5 part.
3. a kind of natural bactericidal agent preventing and treating Shorthairy Antenoron anthracnose according to claim 1, is characterized in that: be made up of the component of following weight portion: 10 parts, madder, frutus cnidii 5 parts, 5 parts, talcum, caulis bambusae in taenian 3 parts, Changshan 3 parts, the Chinese pulsatilla 3 parts, the achene of Siberian cocklebur 2 parts, alkanet 2 parts, indigo naturalis 1 part, Verbena officinalis 1 part.
4. prevent and treat a preparation method for the natural bactericidal agent of Shorthairy Antenoron anthracnose, it is characterized in that: comprise the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is madder, frutus cnidii, talcum, caulis bambusae in taenian, Changshan, the Chinese pulsatilla, the achene of Siberian cocklebur, alkanet, indigo naturalis and Verbena officinalis;
(2) compound is added the ethanol water of 5-10 times amount 60%, be heated to 50-70 DEG C and decoct 2-4h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5-10 times amount 60%, is heated to 50-70 DEG C and decocts 2-4h;
(4) filter, merging filtrate, is concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, leaves standstill 24-48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, must prevent and treat the natural bactericidal agent of Shorthairy Antenoron anthracnose.
5. prevent and treat the application of natural bactericidal agent in control Shorthairy Antenoron anthracnose of Shorthairy Antenoron anthracnose, it is characterized in that: the natural bactericidal agent of control Shorthairy Antenoron anthracnose is mixed with 500-1000 times of water gaging and is sterilization microbial inoculum.
CN201510762175.0A 2015-11-10 2015-11-10 Natural sterilization agent capable of preventing and treating shorthairy antenoron anthracnose and preparation method of natural sterilization agent Pending CN105394105A (en)

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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106116922A (en) * 2016-06-29 2016-11-16 固镇县华丰蔬菜专业合作社 A kind of Fructus Momordicae charantiae dedicated fertilizer preventing and treating Fructus Momordicae charantiae anthrax
CN106116826A (en) * 2016-06-22 2016-11-16 固镇玉鹏生态农业文化园有限公司 A kind of bitter rot or anthracnose of grape control agent
CN107318899A (en) * 2017-07-28 2017-11-07 黔西南州林晨黔农业发展有限公司 A kind of composition of prevention asparagus anthracnose
CN107494630A (en) * 2017-08-22 2017-12-22 紫云苗族布依族自治县亚鲁王文化旅游产业发展有限公司 A kind of composition for treating Fructus Akebiae anthracnose
CN107897215A (en) * 2017-11-27 2018-04-13 桂林斯泰硕农农业科技有限公司 The method for preventing Bu Funa leaf blights evil

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106116826A (en) * 2016-06-22 2016-11-16 固镇玉鹏生态农业文化园有限公司 A kind of bitter rot or anthracnose of grape control agent
CN106116922A (en) * 2016-06-29 2016-11-16 固镇县华丰蔬菜专业合作社 A kind of Fructus Momordicae charantiae dedicated fertilizer preventing and treating Fructus Momordicae charantiae anthrax
CN107318899A (en) * 2017-07-28 2017-11-07 黔西南州林晨黔农业发展有限公司 A kind of composition of prevention asparagus anthracnose
CN107494630A (en) * 2017-08-22 2017-12-22 紫云苗族布依族自治县亚鲁王文化旅游产业发展有限公司 A kind of composition for treating Fructus Akebiae anthracnose
CN107897215A (en) * 2017-11-27 2018-04-13 桂林斯泰硕农农业科技有限公司 The method for preventing Bu Funa leaf blights evil

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