CN103461399B - A kind of natural bactericidal agent preventing and treating ginkgo nursery stock damping-off and preparation method thereof - Google Patents

A kind of natural bactericidal agent preventing and treating ginkgo nursery stock damping-off and preparation method thereof Download PDF

Info

Publication number
CN103461399B
CN103461399B CN201310445466.8A CN201310445466A CN103461399B CN 103461399 B CN103461399 B CN 103461399B CN 201310445466 A CN201310445466 A CN 201310445466A CN 103461399 B CN103461399 B CN 103461399B
Authority
CN
China
Prior art keywords
nursery stock
parts
bactericidal agent
grams
natural bactericidal
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN201310445466.8A
Other languages
Chinese (zh)
Other versions
CN103461399A (en
Inventor
卞佳林
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Li Houde
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CN201310445466.8A priority Critical patent/CN103461399B/en
Publication of CN103461399A publication Critical patent/CN103461399A/en
Application granted granted Critical
Publication of CN103461399B publication Critical patent/CN103461399B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Abstract

A kind of natural bactericidal agent preventing and treating ginkgo nursery stock damping-off provided by the invention, is made up of following component: the seed of feather cockscomb, tarragon, blackberry lily, edible tulip, bluish dogbane, the fruit of Rangoon creeper, fructus schisandrae, moutan bark, dark plum, composite polymineral.This prevents and treats the natural bactericidal agent abundant raw material source of ginkgo nursery stock damping-off, with low cost, adopts the component of pure natural to make, not only Nonpoisonous, non-environmental-pollution, and effectively can prevent and treat ginkgo nursery stock damping-off.

Description

A kind of natural bactericidal agent preventing and treating ginkgo nursery stock damping-off and preparation method thereof
Technical field
The invention belongs to prevention and elimination of disease and pests field, particularly a kind of natural bactericidal agent preventing and treating ginkgo nursery stock damping-off and preparation method thereof.
Background technology
Ginkgo nursery stock damping-off is one of Major Diseases of Seedlings of Ginkgo biloba, all generally occurs in each ginkgo nursery garden.Death of seedling rate is very high, and especially when sowing more late, the incidence of disease is higher.Ginkgo nursery stock damping-off disease occurs more than between 4 ~ June.Because period of disease is different, usually there are four kinds of symptom: 1. plant before real kind of the bud that rots is unearthed and invaded by germ, cause seed decay to be called bud-rot type damping off.2. cauline leaf rots.Young shoot is unearthed period, due to reasons such as humidity are excessive or nursery stock is overstocked, is invaded by germ, causes cauline leaf to bond and rots, claim top rot type sudden disease.3. seedling is dampinged off.Take root period after coming up, because nursery stock degree of lignification is poor, germ invades rhizome, produces brown spot, and it is water stain shape that scab expands, and because germ spreads in seedling stem tissue, causes typical seedling to damping off symptom.4. nursery stock stands withered.After nursery stock stem lignification, germ invades from root, makes butt rot, and sick seedling is withered, but non-lodging, claim the damping-off of seedling.Above-mentioned four kinds of symptoms all have generation, but sudden imperial the most serious with seedling.The producing cause of ginkgo seedling damping off it has been generally acknowledged that and is divided into non-infective and infectivity.Ground, non-invasive disease reason garden ponding, earthing are blocked up, table soil hardens, surface temperature is crossed high reason and caused, can easy prevention and control by improving seedling management.Infectivity cause of disease comprises rhizoctonia fusarium, sickle-like bacteria Rhizoctonia and pythium spp (Pyihium) etc.These three kinds of pathogens all have stronger S, can survive at ordinary times on the plant residue of soil, once run into suitable host and namely wet environment infects harm.Pythium spp is many to be endangered when soil temperature 12 ~ 23 DEG C, and the thermophilic of rhizoctonia 25 ~ 28 DEG C, the thermophilic of sickle-like bacteria is 20 ~ 30 DEG C.The not easily prevention and control of infectivity cause of disease.
In existing ginkgo seedling raising process, be chemical pesticide bactericide for preventing the infectivity cause of disease of damping off normally used, the problem of environmental pollution caused owing to using chemical pesticide is in a large number on the rise.Meanwhile, adopt chemical pesticide bactericide, time various phytopathogen bacterial classification produce drug resistance, pesticide resistance, add the difficulty of control, even cause and prevent and treat unsuccessfully.In addition, chemical pesticide does not have selectivity because of it, also can threaten to useful biology.And a lot of chemical pesticide is non-degradable or is difficult to degraded, easily pollutes the enrichment of thing at animals and plants cylinder accumulation.
Along with the requirement of the public to " pollution-free food ", " pollution-free food " is more and more higher, market to pollution-free, can the ecological agricultural chemical demand of natural degradation also increase gradually.From the development of pesticide science, " Harmless " of " back to nature " and agricultural chemicals on pesticide manufacturing uses, be the inexorable trend of society and Development of Natural Science, therefore new bio wide-spectrum bactericide has wide development prospect.
Summary of the invention
Goal of the invention: the object of this invention is to provide a kind of environmental protection, the natural bactericidal agent preventing and treating ginkgo nursery stock damping-off that bactericidal effect is good.
Technical scheme: a kind of natural bactericidal agent preventing and treating ginkgo nursery stock damping-off provided by the invention, is made up of following component: the seed of feather cockscomb, tarragon, blackberry lily, edible tulip, bluish dogbane, the fruit of Rangoon creeper, fructus schisandrae, moutan bark, dark plum, composite polymineral.
Preferably, be made up of the component of following weight portion: seed of feather cockscomb 4-6 part, tarragon 4-6 part, blackberry lily 4-6 part, edible tulip 4-6 part, bluish dogbane 2-4 part, fruit of Rangoon creeper 1-3 part, fructus schisandrae 1-3 part, moutan bark 1-3 part, dark plum 0.5-1.5 part, composite polymineral 1-3 part.
More preferably, be made up of the component of following weight portion: the seed of feather cockscomb 5 parts, tarragon 5 parts, blackberry lily 5 parts, edible tulip 5 parts, bluish dogbane 3 parts, the fruit of Rangoon creeper 2 parts, fructus schisandrae 2 parts, moutan bark 2 parts, dark plum 1 part, composite polymineral 2 parts.
Wherein, described composite polymineral comprises boron, zinc, molybdenum, manganese, copper, iron, and per kilogram composite polymineral comprises: boron 20 grams, 30 grams, zinc, molybdenum 50 grams, 50 grams, manganese, copper 20 grams, iron 100 grams, surplus is the elements such as carbon, oxygen, nitrogen, phosphorus.
Present invention also offers the above-mentioned preparation method preventing and treating the natural bactericidal agent of ginkgo nursery stock damping-off, comprise the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is the seed of feather cockscomb, tarragon, blackberry lily, edible tulip, bluish dogbane, the fruit of Rangoon creeper, fructus schisandrae, moutan bark, dark plum;
(2) compound is added the ethanol water of 5-10 times amount 95%, be heated to 50-80 DEG C and decoct 2-4h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5-10 times amount 95%, is heated to 50-80 DEG C and decocts 2-4h;
(4) filter, merging filtrate, is concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, leaves standstill 24-48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, and adds composite polymineral mixing, must prevent and treat the natural bactericidal agent of ginkgo nursery stock damping-off.
Present invention also offers the above-mentioned natural bactericidal agent preventing and treating ginkgo nursery stock damping-off and preventing and treating the application in ginkgo nursery stock damping-off, be specially: the natural bactericidal agent preventing and treating ginkgo nursery stock damping-off is mixed with 500-1000 times of water gaging and is sterilization microbial inoculum.
Beneficial effect: natural bactericidal agent abundant raw material source of preventing and treating ginkgo nursery stock damping-off provided by the invention, with low cost, adopt the component of pure natural to make, not only Nonpoisonous, non-environmental-pollution, and effectively can prevent and treat ginkgo nursery stock damping-off.
Embodiment
According to following embodiment, the present invention may be better understood.But those skilled in the art will readily understand, concrete material proportion, process conditions and result thereof described by embodiment only for illustration of the present invention, and should can not limit the present invention described in detail in claims yet.
Embodiment 1
Prevent and treat the natural bactericidal agent of ginkgo nursery stock damping-off, be made up of the component of following weight portion: the seed of feather cockscomb 4 parts, tarragon 5 parts, blackberry lily 4 parts, edible tulip 5 parts, bluish dogbane 2 parts, the fruit of Rangoon creeper 1 part, fructus schisandrae 3 parts, moutan bark 3 parts, dark plum 1.5 parts, composite polymineral 2 parts.Per kilogram composite polymineral comprises: boron 20 grams, 30 grams, zinc, molybdenum 50 grams, 50 grams, manganese, copper 20 grams, iron 100 grams.
Its preparation method, comprises the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is the seed of feather cockscomb, tarragon, blackberry lily, edible tulip, bluish dogbane, the fruit of Rangoon creeper, fructus schisandrae, moutan bark, dark plum;
(2) compound is added the ethanol water of 8 times amount 95%, be heated to 60 DEG C and decoct 3h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 8 times amount 95%, is heated to 60 DEG C and decocts 2-4h;
(4) filter, merging filtrate, be concentrated into that ethanol adds total amount 1/15, add the water of concentrate 2 times of volumes, leave standstill 36h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, and adds composite polymineral mixing, must prevent and treat the natural bactericidal agent of ginkgo nursery stock damping-off.
Embodiment 2
Prevent and treat the natural bactericidal agent of ginkgo nursery stock damping-off, be made up of the component of following weight portion: the seed of feather cockscomb 6 parts, tarragon 6 parts, blackberry lily 6 parts, edible tulip 4 parts, bluish dogbane 4 parts, the fruit of Rangoon creeper 3 parts, fructus schisandrae 1 part, moutan bark 1 part, dark plum 0.5 part, composite polymineral 1 part.Per kilogram composite polymineral comprises: boron 20 grams, 30 grams, zinc, molybdenum 50 grams, 50 grams, manganese, copper 20 grams, iron 100 grams.
Its preparation method, comprises the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is the seed of feather cockscomb, tarragon, blackberry lily, edible tulip, bluish dogbane, the fruit of Rangoon creeper, fructus schisandrae, moutan bark, dark plum;
(2) compound is added the ethanol water of 5 times amount 95%, be heated to 80 DEG C and decoct 4h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5 times amount 95%, is heated to 80 DEG C and decocts 4h;
(4) filter, merging filtrate, be concentrated into that ethanol adds total amount 1/20, add the water of concentrate 1 times of volume, leave standstill 48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, and adds composite polymineral mixing, must prevent and treat the natural bactericidal agent of ginkgo nursery stock damping-off.
Embodiment 3
Prevent and treat the natural bactericidal agent of ginkgo nursery stock damping-off, be made up of the component of following weight portion: the seed of feather cockscomb 5 parts, tarragon 4 parts, blackberry lily 5 parts, edible tulip 6 parts, bluish dogbane 3 parts, the fruit of Rangoon creeper 2 parts, fructus schisandrae 2 parts, moutan bark 2 parts, dark plum 1 part, composite polymineral 3 parts.Per kilogram composite polymineral comprises: boron 20 grams, 30 grams, zinc, molybdenum 50 grams, 50 grams, manganese, copper 20 grams, iron 100 grams.
Its preparation method, comprises the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is the seed of feather cockscomb, tarragon, blackberry lily, edible tulip, bluish dogbane, the fruit of Rangoon creeper, fructus schisandrae, moutan bark, dark plum;
(2) compound is added the ethanol water of 10 times amount 95%, be heated to 50 DEG C and decoct 2h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 10 times amount 95%, is heated to 50 DEG C and decocts 2h;
(4) filter, merging filtrate, be concentrated into that ethanol adds total amount 1/10, add the water of concentrate 3 times of volumes, leave standstill 24h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, and adds composite polymineral mixing, must prevent and treat the natural bactericidal agent of ginkgo nursery stock damping-off.
Embodiment 4 measures the natural bactericidal agent preventing and treating ginkgo nursery stock damping-off of embodiment 1 to 3 to the inhibit activities of strawberry pathogen
This experiment, in vitro, measures the natural bactericidal agent preventing and treating ginkgo nursery stock damping-off of embodiment 1 to 3 to the inhibit activities of strawberry pathogen, method and result as follows:
1. for examination bacterial classification
Sickle-like bacteria (Fusarium sp.) GI115 is commercially available
Rhizoctonia solani Kuhn (Rhizoctonia solani Kuhn) is commercially available
Pythium spp (Py.helicoides) is commercially available
2.EC50 measures
Utilize mycelial growth rate method and spore germination method to measure embodiment of the present invention 1-3 natural bactericidal agent to the EC50 value of above-mentioned germ, the results are shown in Table 1.
Table 1 embodiment 1 natural bactericidal agent is to the EC50 value for examination pathogen
Adopt the method for regulation in " pesticide field efficacy medicine test criterion ", test the field efficacy of the natural bactericidal agent of the embodiment of the present invention 1 to 3, diluted concentration is 500 and 1000 times, the results are shown in Table 2.
The field control effectiveness test of the natural bactericidal agent of table 2 embodiment 1 to 3
Adopt the natural bactericidal agent treatment ginkgo nursery stock damping-off of embodiment 1 to 3, various disease respectively treats 100 strains, and extension rate is 500 times, the results are shown in Table 3.
The natural bactericidal agent treatment strawberry disease of table 3 embodiment 1 to 3

Claims (4)

1. prevent and treat a natural bactericidal agent for ginkgo nursery stock damping-off, it is characterized in that: be made up of the component of following weight portion: seed of feather cockscomb 4-6 part, tarragon 4-6 part, blackberry lily 4-6 part, edible tulip 4-6 part, bluish dogbane 2-4 part, fruit of Rangoon creeper 1-3 part, fructus schisandrae 1-3 part, moutan bark 1-3 part, dark plum 0.5-1.5 part, composite polymineral 1-3 part; Described composite polymineral comprises boron, zinc, molybdenum, manganese, copper, iron, and per kilogram composite polymineral comprises: boron 20 grams, 30 grams, zinc, molybdenum 50 grams, 50 grams, manganese, copper 20 grams, iron 100 grams.
2. a kind of natural bactericidal agent preventing and treating ginkgo nursery stock damping-off according to claim 1, is characterized in that: be made up of the component of following weight portion: the seed of feather cockscomb 5 parts, tarragon 5 parts, blackberry lily 5 parts, edible tulip 5 parts, bluish dogbane 3 parts, the fruit of Rangoon creeper 2 parts, fructus schisandrae 2 parts, moutan bark 2 parts, dark plum 1 part, composite polymineral 2 parts.
3. the preparation method preventing and treating the natural bactericidal agent of ginkgo nursery stock damping-off described in claim 1 or 2, is characterized in that: comprise the following steps:
(1) by raw material mixing after pulverizing, sieving respectively, compound is obtained; Described raw material is the seed of feather cockscomb, tarragon, blackberry lily, edible tulip, bluish dogbane, the fruit of Rangoon creeper, fructus schisandrae, moutan bark, dark plum;
(2) compound is added the ethanol water of 5-10 times amount 95%, be heated to 50-80 DEG C and decoct 2-4h;
(3) filter, filtrate is collected, and filter residue adds the ethanol water of 5-10 times amount 95%, is heated to 50-80 DEG C and decocts 2-4h;
(4) filter, merging filtrate, is concentrated into the 1/20-1/10 that ethanol adds total amount, adds 1-3 times of volume water, leaves standstill 24-48h;
(5) filter, filtrate is concentrated into without alcohol taste, adds same weight water and dissolves, and adds composite polymineral mixing, must prevent and treat the natural bactericidal agent of ginkgo nursery stock damping-off.
4. the natural bactericidal agent preventing and treating ginkgo nursery stock damping-off described in claim 1 or 2 is preventing and treating the application in ginkgo nursery stock damping-off, it is characterized in that: mixed with 500-1000 times of water gaging by the natural bactericidal agent preventing and treating ginkgo nursery stock damping-off and be sterilization microbial inoculum.
CN201310445466.8A 2013-09-26 2013-09-26 A kind of natural bactericidal agent preventing and treating ginkgo nursery stock damping-off and preparation method thereof Expired - Fee Related CN103461399B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201310445466.8A CN103461399B (en) 2013-09-26 2013-09-26 A kind of natural bactericidal agent preventing and treating ginkgo nursery stock damping-off and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201310445466.8A CN103461399B (en) 2013-09-26 2013-09-26 A kind of natural bactericidal agent preventing and treating ginkgo nursery stock damping-off and preparation method thereof

Publications (2)

Publication Number Publication Date
CN103461399A CN103461399A (en) 2013-12-25
CN103461399B true CN103461399B (en) 2015-08-05

Family

ID=49786640

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201310445466.8A Expired - Fee Related CN103461399B (en) 2013-09-26 2013-09-26 A kind of natural bactericidal agent preventing and treating ginkgo nursery stock damping-off and preparation method thereof

Country Status (1)

Country Link
CN (1) CN103461399B (en)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105309499A (en) * 2015-11-18 2016-02-10 无锡市稼宝药业有限公司 Botanical fungicide for damping-off disease
CN106417313A (en) * 2016-09-20 2017-02-22 佛山市盈辉作物科学有限公司 Bactericidal composition containing amisulbrom and fluopicolide
CN107372666A (en) * 2017-08-28 2017-11-24 锦屏县新化乡新化所蔬菜专业合作社 A kind of composition for preventing and treating vegetable seedling samping off and preparation method thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1183901A (en) * 1997-12-09 1998-06-10 贾春健 Liquid farm disinfectant made of Chinese medicinal material
CN1895291A (en) * 2006-06-09 2007-01-17 陈永丽 Nano-Chinese medicinal biological product and its preparation
CN102430099A (en) * 2012-01-04 2012-05-02 揭静华 Life-prolonging essence

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1183901A (en) * 1997-12-09 1998-06-10 贾春健 Liquid farm disinfectant made of Chinese medicinal material
CN1895291A (en) * 2006-06-09 2007-01-17 陈永丽 Nano-Chinese medicinal biological product and its preparation
CN102430099A (en) * 2012-01-04 2012-05-02 揭静华 Life-prolonging essence

Also Published As

Publication number Publication date
CN103461399A (en) 2013-12-25

Similar Documents

Publication Publication Date Title
CN103404541B (en) Natural bactericide for preventing and treating strawberry anthracnose and preparation method of natural bactericide
CN104094950B (en) There is the fungicidal composition of synergism
CN104041538B (en) A kind ofly prevent and treat natural bactericidal agent of strawberry black spot and preparation method thereof
CN103380790B (en) Natural bactericide for preventing and curing peach gummosis and preparation method thereof
CN103329945B (en) Natural bactericide for controlling peach tree anthracnose and preparation method
CN101422162B (en) Plant source preparation for preventing and treating Meloidogyne incognita disease and preparation method thereof
CN103380789B (en) Natural bactericide for preventing and curing pear scab and preparation method thereof
CN104026168A (en) Natural fungicide for controlling grey mould fruit rot of strawberry and preparation method of natural fungicide
CN103518783B (en) Natural bactericide for preventing and controlling ginkgo seedling stem rot and preparation method thereof
CN104041547A (en) Natural bactericide for controlling strawberry leaf spot and preparation method thereof
CN103271094A (en) Compound bactericide
CN107164262A (en) A kind of microbial bacterial agent and its bacterial manure available for controlling crop diseases and insect pests
CN103461401B (en) A kind ofly prevent and treat natural bactericidal agent of ginkgo dead leaf disease and preparation method thereof
CN103461399B (en) A kind of natural bactericidal agent preventing and treating ginkgo nursery stock damping-off and preparation method thereof
CN104381268A (en) Compound bactericide
CN104041530A (en) Natural bactericide for controlling strawberry leaf blight and preparation method thereof
CN103380787A (en) Natural bactericide for preventing and curing nursery stock damping-off and preparation method thereof
CN103300023A (en) Pesticide composition
CN101697725A (en) Pesticide composition containing protective bactericide
CN103548849B (en) Synergistic compound biological bactericide and application thereof
CN103461395B (en) A kind ofly prevent and treat natural bactericidal agent of ginkgo die back disease and preparation method thereof
CN104488891A (en) Powdery mildew control sterilization composition
CN104996492A (en) Natural bactericidal agent for preventing and controlling loquat canker, and preparation method thereof
CN103875670B (en) Compound composition of eugenol and cnidium lactone and application thereof
CN104365649B (en) There is the bactericidal composition of potentiation

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C53 Correction of patent for invention or patent application
CB02 Change of applicant information

Address after: 325200 Wenzhou City, Ruian City, fairy street, the village of the first Village No. 115

Applicant after: Bian Jialin

Address before: 212100, No. 4, No. 54, Henderson Pacific Business Center, Dantu New District, Jiangsu, Zhenjiang

Applicant before: Bian Jialin

COR Change of bibliographic data

Free format text: CORRECT: ADDRESS; FROM: 212100 ZHENJIANG, JIANGSU PROVINCE TO: 325200 WENZHOU, ZHEJIANG PROVINCE

C14 Grant of patent or utility model
GR01 Patent grant
CB03 Change of inventor or designer information
CB03 Change of inventor or designer information

Inventor after: Li Houde

Inventor before: Bian Jialin

TR01 Transfer of patent right
TR01 Transfer of patent right

Effective date of registration: 20180111

Address after: 432500 3 groups in Qingming River Village, Qingming River, Yunmeng County, Xiaogan, Hubei

Patentee after: Li Houde

Address before: 325200 Wenzhou City, Ruian City, fairy street, the village of the first Village No. 115

Patentee before: Bian Jialin

CF01 Termination of patent right due to non-payment of annual fee
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20150805

Termination date: 20190926