CN102365939B - Botanical sterilization preparation and application thereof for controlling anthracnose of grape - Google Patents
Botanical sterilization preparation and application thereof for controlling anthracnose of grape Download PDFInfo
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- CN102365939B CN102365939B CN2011104002040A CN201110400204A CN102365939B CN 102365939 B CN102365939 B CN 102365939B CN 2011104002040 A CN2011104002040 A CN 2011104002040A CN 201110400204 A CN201110400204 A CN 201110400204A CN 102365939 B CN102365939 B CN 102365939B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
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Abstract
The invention discloses a botanical sterilization preparation, which contains the following components in percentage by weight: 0.1-50% of thymol, 5-30% of emulsifier, 0.1-20% of azone, 10-60% of ethanol, and 0.1-84.8% of water. The invention further discloses a preparation method and application of the botanical sterilization preparation of the botanical sterilization preparation to control of the bitter rot or anthracnose of grape. Pharmaceutical components of the botanical sterilization preparation based on thymol come from plants or extractives thereof with an insecticidal function; the botanical sterilization preparation not only can be used for effectively controlling the bitter rot or anthracnose of grape but also can be used for promoting growth and development of crops, increasing production, increasing income, improving quality, increasing nutrient utilization rate, controlling injurious insects, avoiding pollution and the like; and the botanical sterilization preparation is applied to the production of nuisanceless, green and organic agricultural products.
Description
Technical field
The invention belongs in the agricultural production fruits and vegetables technical field of preventing and curing diseases, be specifically related to a kind of botanical sterilization preparation and the application in preventing and treating bitter rot or anthracnose of grape thereof.
Background technology
Bitter rot or anthracnose of grape (Grape anthracnose) has another name called late blight, is one of important disease during grape is produced.Anthracnose is that Deuteromycotina belongs to, and germ mainly hides at positions such as the annual complicated and confused textura epidermoidea of being infected, leaf scars with mycelium, and is attached to the invalid tissues such as deadwood, fallen leaves, decayed fruit with conidium and survives the winter.Generally only occur on the painted or maturescent fruit, but germ can be infected green fruit, climing, leaf and tendril etc., and tissue does not show obvious symptom.Fruit morbidity after painted, just at the fleck of the large brown circle of fruit face generation syringe needle, spot enlarged gradually afterwards, and depression, grew gradually the pore that wheel line shape is arranged on the surface.When weather was moist, growing pink shot-like particle on the scab was the conidium agglomerate of germ.Fall ill when serious, scab can expand to half or whole fruit face, and the soft corruption of fruit grain easily come off, or drying shrinkage becomes mummy gradually.Carpopodium and cob morbidity produce the scab that the crineous Long Circle caves in, and cause fruit to rot in a large number, cause serious loss.
Preventing and treating on the means of bitter rot or anthracnose of grape at present, main or chemical control, agricultural chemicals commonly used mainly is mancozeb, tmtd, carbendazim.Efficient, quick-acting, easy to use, economic benefit advantages of higher that bactericide has, but only have protective effect, nothing is treated and is rooted out effect, and the lasting period is short, and application times is many, and germ has produced pesticide resistance to it; Long-time unreasonable use chemical pesticide control bitter rot or anthracnose of grape causes pathogen to develop immunity to drugs gradually, and control efficiency reduces, and can increase the fruit Pesticide Residues, and contaminated environment increases production cost.Chemical pesticide still is difficult to effectively solve to people and animals' toxic and side effect and residue problem so far.
Along with the appearance of more and more pesticide resistance phytopathogens, seek that other is alternative, toxic and side effect is little, agricultural chemicals has become the focal issue of agricultural sustainable development safely and effectively.The characteristics such as it is safe, efficient, pollution-free that botanical biological pesticide has and are preserved the ecological environment and food safety production requires to match.And by the biopesticide of making after the improvement of vegetable source natural compound, not only control efficiency is good, also to fruit, flower, the Ye Douwu harm of plant, to the person poultry safety, has efficient noresidue, without poisoning, and the advantages such as the non-resistant property of medicine; Simultaneously can the Effective Raise quality of agricultural product in the control disease.
Summary of the invention
Technical problem to be solved by this invention provides a kind of botanical sterilization preparation based on thymol, and said preparation is a kind of friendly preparation of many effects novel environmental that integrates antibiotic, nutrition, improves quality.
The technical problem that the present invention also will solve provides the preparation method of above-mentioned botanical sterilization preparation based on thymol.
The technical problem that the present invention will solve at last provides the application of above-mentioned botanical sterilization preparation in preventing and treating bitter rot or anthracnose of grape.
For solving the problems of the technologies described above, the technical solution used in the present invention is as follows:
A kind of botanical sterilization preparation, it comprises the component of following percetage by weight: thymol 0.1～50%, emulsifier 5～30%, azone 0.1～20%, ethanol 10～60%, water 0.1～84.8%.
Above-mentioned botanical sterilization preparation preferably comprises the component of following percetage by weight: thymol 0.5～5%, and emulsifier 10～20%, azone 2～3%, ethanol 20～40%, water 32～67.5%, and described botanical sterilization preparation contains Trichoderma 1 * 10
8Individual/ml.
Wherein, described emulsifier is one or more the mixture in phenethyl phenol polyethenoxy ether, phenethyl phenol polyoxyethylene poly-oxygen propylene aether, aralkyl phenol polyethenoxy ether phosphate, alkylphenol polyoxyethylene phosphate, alkyl naphthalene sulfonic acid calcium, castor oil ethylene oxide adduct, aralkyl phenol polyethenoxy ether, fatty alcohol-polyoxyethylene ether, alkyl phenol formaldehyde resin polyoxyethylene ether and the dialkyl benzene sulfonic acids calcium.
Above-mentioned thymol can carry out composite with carbendazim, Tebuconazole.
The preparation method of above-mentioned botanical sterilization preparation, press formula ratio and prepare raw material thymol, emulsifier, azone, second alcohol and water, thymol added mix the formation solution A in the part ethanol, again emulsifier and azone are formed solution B with the dissolving of residue ethanol, then solution A, solution B and water are mixed.
Wherein, ethanol uses (being above-mentioned part ethanol and above-mentioned residue ethanol) at twice, the abundant dissolving of each used in amounts raw material that assurance is dissolved, and twice consumption sum is the ethanol raw material total amount of formula ratio.
The application of above-mentioned botanical sterilization preparation in promoting grape growth.
The application of above-mentioned botanical sterilization preparation in preventing and treating bitter rot or anthracnose of grape.
Beneficial effect: its effective component of botanical sterilization preparation based on thymol of the present invention derives from plant or its extract with insecticidal function, can not only effectively prevent and treat bitter rot or anthracnose of grape, have simultaneously promote crop growth, increasing both production and income, improve quality, improve plant recovery of nutrient, pest control, the multi-efficiency such as pollution-free, be applicable to nuisanceless, green and organic farm products production.The said preparation effect significantly, raw material are easy to get, and production technology is simple, market potential is large, economic benefit and social benefit are obvious, has very wide application prospect.
Embodiment
According to following embodiment, the present invention may be better understood.Yet, those skilled in the art will readily understand, embodiment is described only to be used for explanation the present invention, and should also can not limit the present invention described in detail in claims.
Embodiment 1:
A kind of botanical sterilization preparation, it comprises the component of following percetage by weight: thymol 2%, emulsifier (emulsifier is phenethyl phenol polyoxyethylene poly-oxygen propylene aether) 20%, azone 2%, ethanol 30%, water 46%, and described botanical sterilization preparation contains Trichoderma 1 * 10
8Individual/ml.
The preparation method of above-mentioned botanical sterilization preparation, press formula ratio and prepare raw material thymol, emulsifier, azone, second alcohol and water, mix the formation solution A in the ethanol with thymol adding 1/4 amount, again emulsifier and azone are formed solution B with the ethanol dissolving that remains 3/4 amount, then solution A, solution B and water are mixed.
Embodiment 2:
A kind of botanical sterilization preparation, it comprises the component of following percetage by weight: thymol 1%, emulsifier (emulsifier is dialkyl benzene sulfonic acids calcium) 10%, azone 2%, ethanol 20%, water 67%, and described botanical sterilization preparation contains Trichoderma 1 * 10
8Individual/ml.
The preparation method of above-mentioned botanical sterilization preparation, press formula ratio and prepare raw material thymol, emulsifier, azone, second alcohol and water, mix the formation solution A in the ethanol with thymol adding 1/2 amount, again emulsifier and azone are formed solution B with the ethanol dissolving that remains 1/2 amount, then solution A, solution B and water are mixed.
Embodiment 3:
A kind of botanical sterilization preparation, it comprises the component of following percetage by weight: thymol 0.5%, emulsifier (emulsifier is phenethyl phenol polyethenoxy ether and alkylphenol polyoxyethylene phosphate by weight 1: 2 mixture) 10%, azone 2%, ethanol 20%, water 67.5%, and described botanical sterilization preparation contains Trichoderma 1 * 10
8Individual/ml.
The preparation method is with embodiment 2.
Embodiment 4:
A kind of botanical sterilization preparation, it comprises the component of following percetage by weight: thymol 0.1%, emulsifier (emulsifier is alkyl naphthalene sulfonic acid calcium, castor oil ethylene oxide adduct and aralkyl phenol polyethenoxy ether by weight 1: 1: 1 mixture) 30%, azone 20%, ethanol 10%, water 39.9%, and described botanical sterilization preparation contains Trichoderma 1 * 10
8Individual/ml.
The preparation method is with embodiment 2.
Embodiment 5:
A kind of botanical sterilization preparation, it comprises the component of following percetage by weight: thymol 50%, emulsifier (emulsifier is fatty alcohol-polyoxyethylene ether, alkyl phenol formaldehyde resin polyoxyethylene ether and dialkyl benzene sulfonic acids calcium by weight 1: 1: 1 mixture) 5%, azone 0.1%, ethanol 20%, water 24.9%, and described botanical sterilization preparation contains Trichoderma 1 * 10
8Individual/ml.
The preparation method is with embodiment 2.
Embodiment 6: based on the botanical sterilization preparation of thymol to the promotion of plant growth with to the improvement effect of quality.
Formula I: 1/2Hoagland nutrient solution.
The botanical sterilization preparation of the thymol that formula I I: embodiment 1 makes.
Choose 3 processing of dimension design of farmland: process 1: contrast (contrast CK is clear water); Process 2: formula I; Process 3: formula I I.Each is processed and repeats 3 times, and treatment fluid sprays once every 5d, sprays continuously 3 times, waits for that gathering in the crops rear sampling analyzes.
Assay method:
Pericarp anthocyanin content: get each and repeat 20 of cold storage fruits, drill through about 30 on fruit grain equatorial plane pericarp with card punch, mixing is weighed, and adds 2% hydrochloric acid-ethanol 4mL in the 10mL centrifuge tube, places 32 ℃ of insulating box dippings 2 hours, then take out centrifuge tube and descended 8000 * g centrifugal 10 minutes at 4 ℃, collect supernatant, add again 2% hydrochloric acid-ethanol 3mL lixiviate, repeat to extract 3 times, merge supernatant, constant volume is to 10mL.Measure the content of anthocyanin with pH differential methods such as Wang Shaobo.(Wang Shaobo, Du Yongfeng, Yao grasp the anthocyan in the magnificent .pH differential method mensuration Testa sojae atricolor. chemical analysis metering, 2008 (1): 46-49)
Fruit juice soluble solid, titratable acid and pH value: break into homogenate with beater after the sample peeling, be divided in 3 centrifuge tubes, 8000 * g centrifugal 10 minutes under 4 ℃, get the standby survey of supernatant.Soluble solid is measured with electronic digital display type saccharometer (DR103).Titratable acid content is measured with the acid base neutralization titration method, and is converted into tartaric acid content.The pH value is measured with pH meter.Specifically measure with reference to methods such as Liu Ailings.(what is for army building, Wang Chenghua, Li Weifu, Xie Zhaosen, old Yu, Shen Yuliang, Wang Shiping for Liu Ailing, Lu Zhixing. and nutrient solution concentration is on the impact of " behind the peak " developmental stages of grape berry and quality, southern china fruit tree .2011,40 (4): 74-77.)
Table 1 different disposal is on the impact of grape biology amount and quality
As shown in Table 1, grape spike number, Dan Suichong, sugar-acid ratio, soluble solid content and pericarp anthocyanin content after formula I is processed increased by 5.6%, 10.3%, 4.1%, 10.3% and 8.8% than contrast respectively; Spike number, Dan Suichong, sugar-acid ratio, soluble solid content and pericarp anthocyanin content had increased by 14.9%, 19.6%, 16.6%, 13.5% and 17.6% than contrast respectively after formula I I processed.
On having as can be known, with formula I I process to grape biology amount and quality to improve effect best.
Embodiment 7: based on the botanical sterilization preparation of thymol at the preventive and therapeutic effect to bitter rot or anthracnose of grape.
Formula I: 50% carbendazol wettable powder.
Formula I I:75% tpn wetting powder.
The botanical sterilization preparation of the thymol that formula I II: embodiment 1 makes.
Choose four processing of morbidity dimension design of farmland: process 1: contrast (contrast CK is clear water); Process 2: formula I; Process 3: formula I I; Process 4: formula I II.Each process to repeat 3 times, and treatment fluid sprays once every 5d, carries out statistical analysis after spraying 3 times.
Assay method:
The mensuration of disease index and control efficiency: with reference to Dou Rui flitch method.(Dou Rui wood .3 plants endogenetic bacterium is to the control efficiency of graw mold of tomato. Henan Agricultural Sciences, and 2010,4:77-79.)
As shown in Table 2, on grape, formula I is 37.00% to the preventive effect of bitter rot or anthracnose of grape; Formula I I is 47.80% to the preventive effect of bitter rot or anthracnose of grape; The preventive effect of formula I II bitter rot or anthracnose of grape is 78.68%.
As from the foregoing, process best to the control efficiency of bitter rot or anthracnose of grape with formula I I.
Table 2 different disposal is to the bitter rot or anthracnose of grape control efficiency
Claims (2)
1. the application of botanical sterilization preparation in promoting grape growth; Wherein, described botanical sterilization preparation, it comprises the component of following percetage by weight: thymol 0.1 ~ 50%, emulsifier 5 ~ 30%, azone 0.1 ~ 20%, ethanol 10 ~ 60%, water 0.1 ~ 84.8%; Wherein, described emulsifier is one or more the mixture in phenethyl phenol polyethenoxy ether, phenethyl phenol polyoxyethylene poly-oxygen propylene aether, aralkyl phenol polyethenoxy ether phosphate, alkylphenol polyoxyethylene phosphate, alkyl naphthalene sulfonic acid calcium, castor oil ethylene oxide adduct, aralkyl phenol polyethenoxy ether, fatty alcohol-polyoxyethylene ether, alkyl phenol formaldehyde resin polyoxyethylene ether and the dialkyl benzene sulfonic acids calcium.
2. the application of botanical sterilization preparation in preventing and treating bitter rot or anthracnose of grape; Wherein, described botanical sterilization preparation, it comprises the component of following percetage by weight: thymol 0.1 ~ 50%, emulsifier 5 ~ 30%, azone 0.1 ~ 20%, ethanol 10 ~ 60%, water 0.1 ~ 84.8%; Wherein, described emulsifier is one or more the mixture in phenethyl phenol polyethenoxy ether, phenethyl phenol polyoxyethylene poly-oxygen propylene aether, aralkyl phenol polyethenoxy ether phosphate, alkylphenol polyoxyethylene phosphate, alkyl naphthalene sulfonic acid calcium, castor oil ethylene oxide adduct, aralkyl phenol polyethenoxy ether, fatty alcohol-polyoxyethylene ether, alkyl phenol formaldehyde resin polyoxyethylene ether and the dialkyl benzene sulfonic acids calcium.
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| CN2011104002040A CN102365939B (en) | 2011-12-06 | 2011-12-06 | Botanical sterilization preparation and application thereof for controlling anthracnose of grape |
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| CN2011104002040A CN102365939B (en) | 2011-12-06 | 2011-12-06 | Botanical sterilization preparation and application thereof for controlling anthracnose of grape |
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| CN102365939B true CN102365939B (en) | 2013-04-17 |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102365939B (en) * | 2011-12-06 | 2013-04-17 | 江苏省农业科学院 | Botanical sterilization preparation and application thereof for controlling anthracnose of grape |
| CN109258637A (en) * | 2018-10-17 | 2019-01-25 | 四川省兰月科技有限公司 | Sterilize the composition pesticide of control shoot |
| CN109526530B (en) * | 2018-11-21 | 2021-06-08 | 靳职雄 | Planting method of pollution-free grapes |
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| JP2001310214A (en) * | 2000-04-28 | 2001-11-06 | Aubex Corp | Lengthy material working device and lengthy material working method |
| CN1357229A (en) * | 2000-12-13 | 2002-07-10 | 刘岱 | Application, production and processing of elsholtzia herb as natural plant pest and disease preventing and eliminating agent |
| CN102365939A (en) * | 2011-12-06 | 2012-03-07 | 江苏省农业科学院 | Botanical sterilization preparation and application thereof for controlling bitter rot or anthracnose of grape |
| CN102365940A (en) * | 2011-12-06 | 2012-03-07 | 江苏省农业科学院 | Botanical sterilization preparation and application thereof for controlling pepper anthracnose |
| CN102388869A (en) * | 2011-12-06 | 2012-03-28 | 江苏省农业科学院 | Botanical fungicide and its application in control of strawberry botrytis cinerea |
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|---|---|---|---|---|
| JP2001310214A (en) * | 2000-04-28 | 2001-11-06 | Aubex Corp | Lengthy material working device and lengthy material working method |
| CN1357229A (en) * | 2000-12-13 | 2002-07-10 | 刘岱 | Application, production and processing of elsholtzia herb as natural plant pest and disease preventing and eliminating agent |
| CN102365939A (en) * | 2011-12-06 | 2012-03-07 | 江苏省农业科学院 | Botanical sterilization preparation and application thereof for controlling bitter rot or anthracnose of grape |
| CN102365940A (en) * | 2011-12-06 | 2012-03-07 | 江苏省农业科学院 | Botanical sterilization preparation and application thereof for controlling pepper anthracnose |
| CN102388869A (en) * | 2011-12-06 | 2012-03-28 | 江苏省农业科学院 | Botanical fungicide and its application in control of strawberry botrytis cinerea |
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