Disclosure of Invention
As a result of a variety of extensive and intensive studies and experiments, the inventors of the present invention have found that the plukenetia volubilis linneo extract has a superior moisturizing effect on grape seedlings, and can increase the survival rate of the grape seedlings. Based on this finding, the present invention has been completed.
An object of the present invention is to solve at least the above problems and/or disadvantages and to provide at least the advantages described hereinafter.
Still another object of the present invention is to provide a method for planting pollution-free grapes, which can effectively enrich selenium in grapes.
To achieve these objects and other advantages in accordance with the present invention, there is provided a method for planting pollution-free grapes, comprising the steps of:
1) selecting a field with a good soil structure, deeply ploughing the land by 50-60cm, mixing 1000-1800 kg of decomposed animal excrement and 50-100kg of traditional Chinese medicine bactericide into each mu of land during ploughing, ridging the planting land along rows with the row spacing of 2-2.3m, and building a grape vine-climbing frame along the rows, wherein the traditional Chinese medicine bactericide comprises the following raw material components in parts by weight: 10-15 parts of weak acid electrolyzed water, 30-50 parts of absolute ethyl alcohol, 10-15 parts of ginger powder, 13-16 parts of perilla leaf, 6-9 parts of folium isatidis and 3-5 parts of dandelion; the preparation method of the decomposed animal excrement comprises the following steps: crushing water hyacinth to water content of 8-12% to 1-10mm to obtain crushed water hyacinth material, mixing the crushed water hyacinth material, oyster shell powder and animal excrement with water content of 40-50%, adding EM microbial inoculum for fermenting and decomposing for 30-40 days, adding nitrogen fertilizer, phosphate fertilizer and potassium fertilizer, and mixing uniformly to obtain decomposed animal excrement;
2) selecting excellent grape saplings, soaking the grape saplings in warm water for 60-80min, then soaking the grape saplings in 75% alcohol solution for 3-5min, taking out the grape saplings after being placed in the sun, soaking the grape saplings in the Plukenetia volubilis fruit extract for 2-3h, taking out the grape saplings, uniformly dipping plant ash on the roots of the grape saplings, planting the grape saplings at a plant spacing of 85-95 cm between every two adjacent grapes in each row, laying hay around the grape saplings, and spraying a traditional Chinese medicine insecticide onto the hay, wherein the traditional Chinese medicine insecticide comprises the following raw materials in parts by weight: 3-5 parts of pyrethrum, 10-13 parts of tobacco leaves, 8-12 parts of allicin, 12-15 parts of gynostemma pentaphylla, 6-9 parts of menthol, 6-9 parts of rhizoma arisaematis, 13-15 parts of golden cypress, 10-12 parts of hawthorn flowers, 12-14 parts of chinaberry fruits and 11-13 parts of rutin;
3) after the grape vines germinate to 20-30cm, spraying a new high-lipid film with the concentration of 10% -15%, thymol and azadirachtin mixed solution to the leaf surfaces of the grape vines every 10-15 days, and pruning the grape vines when the grape vines grow to 200-230 cm, so that the lengths of the grape vines are controlled to be 170-200 cm;
4) after the grape vines are planted, organic liquid fertilizer is dripped to the roots of the grape vines every 15-20 days, 0.2-0.3kg of the organic liquid fertilizer is applied to each grape vine, chitin liquid fertilizer and abamectin are dripped to the roots of the grape vines every 10-12 days after the grapes blossom, rotten animal manure is applied to the roots every 20-25 days, when the illumination intensity in the morning is more than 4000 luxes, weak acid electrolytic water with the pH value of 6.5 and the effective chlorine concentration of 10-15mg/L is used for carrying out foliage spraying and continuous planting, and after the grapes mature, the grapes are picked to obtain the grapes.
Preferably, the preparation method of the traditional Chinese medicine bactericide comprises the following steps: crushing the perilla leaves, the indigowoad leaves and the dandelion in parts by weight, uniformly mixing the crushed perilla leaves, the indigowoad leaves and the dandelion with the ginger powder in parts by weight, putting the mixture into a beaker, adding the absolute ethyl alcohol in parts by weight, heating the mixture in a water bath to 40-50 ℃, dripping the weak acid electrolyzed water in parts by weight under the stirring condition, and extracting for 30-40 min to obtain the traditional Chinese medicine bactericide.
Preferably, in the step 2), the temperature of the warm water is 50-55 ℃.
Preferably, the preparation method of the plukenetia volubilis linneo extract comprises the following steps: airing the plukenetia volubilis linneo until the water content is lower than 8%, adding an ethanol solution which is 4-6 times of the mass of the plukenetia volubilis linneo and has the mass fraction of more than 75%, performing reflux extraction for 60-80min at the temperature of 70-80 ℃, cooling to room temperature, filtering to obtain a filtrate, performing rotary evaporation drying on the filtrate until the mass of the plukenetia volubilis linneo is 0.5-1 time of the mass of the plukenetia volubilis linneo, adding a chitosan solution with the mass fraction of 3-5%, and uniformly stirring to obtain a plukenetia volubilis linneo extract: dissolving 3-5 parts by weight of chitosan into 100 parts by weight of acetic acid solution with the mass fraction of 1-2%, stirring until the solution is clear, and adjusting the pH value of filtrate to be neutral after filtering to obtain the chitosan solution.
Preferably, the preparation method of the traditional Chinese medicine pesticide comprises the following steps: adding water with the mass of 3-5 times of that of the medicinal materials into the pyrethrum, the tobacco leaves, the garlicin, the gynostemma pentaphylla, the menthol, the arisaema consanguineum, the phellodendron amurense, the hawthorn flowers, the chinaberry fruits and the rutin in parts by weight respectively, decocting for 1-2 hours, combining decoction, and performing rotary evaporation at the temperature of 80-90 ℃ until the mass of the decoction is 1-1.5 times of the total mass of the medicinal materials to obtain the traditional Chinese medicine pesticide.
Preferably, the mass ratio of the new high-fat film, the thymol and the azadirachtin is 10-12:2-3: 3-5.
Preferably, in the step 4), 200g of chitin liquid fertilizer, 20-30g of abamectin and 0.5-1kg of decomposed animal manure are added into each tree in a dropwise manner.
The invention at least comprises the following beneficial effects: the traditional Chinese medicine bactericide is added during planting, a new high-fat film, a mixed solution of thymol and azadirachtin and root dropwise adding are sprayed on leaf surfaces in a growing period, and chitin liquid fertilizer, abamectin and decomposed animal manure are added in a later period, so that anthracnose, brown spot, downy mildew and white rot of grapes can be avoided in the planting process; weak acid electrolyzed water is used for soaking the grape saplings, the grape saplings are detoxified, and the occurrence of spike stalk brown blight of the grapes is avoided; the grapevine saplings are soaked by the plukenetia volubilis linneo extract, so that the water retention of the grapevine saplings can be enhanced, the occurrence of root rot can be prevented after the grapevine saplings are dipped with plant ash, and the survival rate of the grapevine saplings can be increased by more than 30%; by spraying the traditional Chinese medicine insecticide on the hay during planting and spraying the mixed solution of the new high-fat film, the thymol and the azadirachtin on the leaf surfaces in the growing period, the insect pests such as aphids, mites and swans can be killed or repelled, and the damage to grapes can be avoided; the occurrence of the late rot can be effectively prevented by spraying weak acid electrolyzed water in the period with illumination. The grapes planted by the method have the characteristics of high yield, good quality and the like.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.
Detailed Description
The present invention is described in further detail below to enable those skilled in the art to practice the invention with reference to the description.
It will be understood that terms such as "having," "including," and "comprising," as used herein, do not preclude the presence or addition of one or more other elements or groups thereof.
Example 1
A planting method of pollution-free grapes comprises the following steps:
1) selecting a field with a good soil structure, deeply ploughing the soil by 50cm, mixing 1000kg of decomposed animal excrement and 50kg of traditional Chinese medicine bactericide into each mu of the field when ploughing, ridging the planting field along rows, wherein the row spacing is 2m, and erecting a grape vine-climbing shelf along the rows, wherein the traditional Chinese medicine bactericide comprises the following raw material components by weight: 10kg of weak acid electrolyzed water, 30kg of absolute ethyl alcohol, 10kg of ginger powder, 13kg of perilla leaf, 6kg of dyers woad leaf and 3kg of dandelion; the preparation method of the decomposed animal excrement comprises the following steps: the method comprises the steps of (1) crushing water hyacinth to the water content of 8% to 10mm to obtain water hyacinth crushed material, uniformly mixing the water hyacinth crushed material, oyster shell powder and animal manure with the water content of 50%, adding EM microbial inoculum for fermenting and decomposing for 30 days, adding nitrogen fertilizer, phosphate fertilizer and potassium fertilizer, and uniformly mixing to obtain decomposed animal manure;
2) selecting excellent grape saplings, soaking the grape saplings in warm water for 60min, then soaking the grape saplings in 75% alcohol solution for 3min, taking out the grape saplings, placing the grape saplings in the sun, drying the grape saplings in the sun, soaking the grape saplings for 2h by using the embelia meiliana extract, taking out the grape saplings, uniformly dipping plant ash on the roots of the grape saplings, planting the grape saplings at a plant spacing of 85cm between every two adjacent grapes in each row, paving hay around the grape saplings, and spraying a traditional Chinese medicine insecticide on the hay, wherein the traditional Chinese medicine insecticide comprises the following raw materials in parts by weight: 3kg of pyrethrum, 10kg of tobacco leaves, 8kg of allicin, 12kg of gynostemma pentaphylla, 6kg of menthol, 6kg of rhizoma arisaematis, 13kg of golden cypress, 10kg of hawthorn flowers, 12kg of chinaberry seeds and 11kg of rutin;
3) after the grapevine sprouts to 20cm, spraying a new high-fat film with the concentration of 10%, thymol and azadirachtin mixed solution to the grapevine leaf surfaces every 10 days, and pruning the grapevine seedlings when the grapevine seedlings grow to 200cm, so that the lengths of the grapevine seedlings are controlled to be 170 cm;
4) after the grape vines are planted, organic liquid fertilizer is dripped to the roots of the grape vines every 15 days, 0.2kg of organic liquid fertilizer is applied to each grape vine, chitin liquid fertilizer and abamectin are dripped to the roots of the grape vines every 10 days after the grapes blossom, rotten animal manure is applied to the roots every 20 days, when the illumination intensity in the morning is more than 4000 lux, weak acid electrolyzed water with the pH value of 6.5 and the effective chlorine concentration of 10mg/L is used for foliage spraying and continuous planting, and the grapes are picked after being ripe, so that the grapes are obtained.
The grapes planted in the embodiment are uniform, each bunch is about 2.5 jin, the yield per mu is 4306 jin, and compared with the common planting method, the yield is improved by more than 9%.
Example 2
A planting method of pollution-free grapes comprises the following steps:
1) selecting a field with a good soil structure, deeply ploughing the soil by 50cm, mixing 1000kg of decomposed animal excrement and 50kg of traditional Chinese medicine bactericide into each mu of the field when ploughing, ridging the planting field along rows, wherein the row spacing is 2m, and erecting a grape vine climbing shelf along the rows, wherein the preparation method of the traditional Chinese medicine bactericide comprises the following steps: crushing 13kg of perilla leaf, 6kg of folium isatidis and 3kg of dandelion, uniformly mixing with 10kg of ginger powder, putting the mixture into a beaker, adding 30kg of absolute ethyl alcohol, heating the mixture in a water bath to 40 ℃, dropwise adding 10kg of weak acid electrolyzed water under the stirring condition, and extracting for 30min to obtain the traditional Chinese medicine bactericide; the preparation method of the decomposed animal excrement comprises the following steps: the method comprises the steps of (1) crushing water hyacinth to the water content of 8% to 10mm to obtain water hyacinth crushed materials, uniformly mixing the water hyacinth crushed materials, oyster shell powder and animal manure with the water content of 50%, adding EM microbial inoculum for fermenting and decomposing for 30 days, adding nitrogen fertilizer, phosphate fertilizer and potassium fertilizer, and uniformly mixing to obtain decomposed animal manure;
2) selecting excellent grape saplings, soaking the grape saplings in warm water at 50 ℃ for 60min, then soaking the grape saplings in 75% alcohol solution for 3min, taking out the grape saplings, placing the grape saplings in the sun for drying, soaking the grape saplings in the Plukenetia volubilis fruit extract for 2h, taking out the grape saplings, uniformly dipping plant ash on the roots of the grape saplings, planting the grape saplings at a plant spacing of 85cm between two adjacent grapes in each row, laying hay around the grape saplings, and spraying a traditional Chinese medicine insecticide onto the hay, wherein the traditional Chinese medicine insecticide comprises the following raw materials in parts by weight: 3kg of pyrethrum, 10kg of tobacco leaves, 8kg of allicin, 12kg of gynostemma pentaphylla, 6kg of menthol, 6kg of rhizoma arisaematis, 13kg of golden cypress, 10kg of hawthorn flowers, 12kg of chinaberry seeds and 11kg of rutin;
3) after the grapevine sprouts to 20cm, spraying a new high-fat film with the concentration of 10%, thymol and azadirachtin mixed solution to the grapevine leaf surfaces every 10 days, and pruning the grapevine seedlings when the grapevine seedlings grow to 200cm, so that the lengths of the grapevine seedlings are controlled to be 170 cm;
4) after the grape vines are planted, organic liquid fertilizer is dripped to the roots of the grape vines every 15 days, 0.2kg of organic liquid fertilizer is applied to each grape vine, chitin liquid fertilizer and abamectin are dripped to the roots of the grape vines every 10 days after the grapes blossom, rotten animal manure is applied to the roots every 20 days, when the illumination intensity in the morning is larger than 4000 lux, weak acid electrolyzed water with the pH value of 6.5 and the effective chlorine concentration of 15mg/L is used for foliage spraying and continuous planting, and the grapes are picked after being ripe, so that the grapes are obtained.
In the embodiment, spot blight, brown spot, downy mildew and white rot do not appear in the planting process, but the outbreak rate of the diseases of the grapes planted by adopting the common planting method is more than 17%.
Example 3
A planting method of pollution-free grapes comprises the following steps:
1) selecting a field with a good soil structure, deeply ploughing the soil by 50cm, mixing 1000kg of decomposed animal excrement and 50kg of traditional Chinese medicine bactericide into each mu of the field when ploughing, ridging the planting field along rows, wherein the row spacing is 2m, and erecting a grape vine climbing shelf along the rows, wherein the preparation method of the traditional Chinese medicine bactericide comprises the following steps: crushing 13kg of perilla leaf, 6kg of folium isatidis and 3kg of dandelion, uniformly mixing with 10kg of ginger powder, putting the mixture into a beaker, adding 30kg of absolute ethyl alcohol, heating the mixture in a water bath to 40 ℃, dropwise adding 10kg of weak acid electrolyzed water under the stirring condition, and extracting for 30min to obtain the traditional Chinese medicine bactericide; the preparation method of the decomposed animal excrement comprises the following steps: the method comprises the steps of (1) crushing water hyacinth to the water content of 8% to 10mm to obtain water hyacinth crushed materials, uniformly mixing the water hyacinth crushed materials, oyster shell powder and animal manure with the water content of 50%, adding EM microbial inoculum for fermenting and decomposing for 30 days, adding nitrogen fertilizer, phosphate fertilizer and potassium fertilizer, and uniformly mixing to obtain decomposed animal manure;
2) selecting excellent grape saplings, soaking the grape saplings in warm water for 60min, then soaking the grape saplings in 75% alcohol solution for 3min, taking out the grape saplings, placing the grape saplings in the sun for drying, then soaking the grape saplings for 2h by using the Plukenetia volubilis fruit extract, taking out the grape saplings, uniformly dipping plant ash on the roots of the grape saplings, planting the grape saplings at a plant spacing of 85cm between two adjacent grapes in each row, laying hay around the grape saplings, and spraying a traditional Chinese medicine insecticide onto the hay, wherein the traditional Chinese medicine insecticide comprises the following raw material components: pyrethrum, tobacco leaves, allicin, gynostemma pentaphylla, menthol, rhizoma arisaematis, phellodendron, hawthorn flowers, chinaberry seeds and rutin;
3) after the grapevine sprouts to 20cm, spraying a new high-fat film with the concentration of 10%, thymol and azadirachtin mixed solution to the grapevine leaf surfaces every 10 days, and pruning the grapevine seedlings when the grapevine seedlings grow to 200cm, so that the lengths of the grapevine seedlings are controlled to be 170 cm;
4) after the grape vines are planted, organic liquid fertilizer is dripped to the roots of the grape vines every 15 days, 0.2kg of organic liquid fertilizer is applied to each grape vine, chitin liquid fertilizer and abamectin are dripped to the roots of the grape vines every 10 days after the grapes blossom, rotten animal manure is applied to the roots every 20 days, when the illumination intensity in the morning is more than 4000 lux, weak acid electrolyzed water with the pH value of 6.5 and the effective chlorine concentration of 15mg/L is used for foliage spraying and continuous planting, and the grapes are picked after being ripe, so that the grapes are obtained.
Compared with the method that the grape saplings are not detoxified by the Redomil, the incidence rate of the grape spike-stalk brown blight is reduced by more than 69%.
Example 4
A planting method of pollution-free grapes comprises the following steps:
1) selecting a field with a good soil structure, deeply ploughing the soil by 50cm, mixing 1000kg of decomposed animal excrement and 50kg of traditional Chinese medicine bactericide into each mu of the field when ploughing, ridging the planting field along rows, wherein the row spacing is 2m, and erecting a grape vine climbing shelf along the rows, wherein the preparation method of the traditional Chinese medicine bactericide comprises the following steps: crushing 13kg of perilla leaf, 6kg of folium isatidis and 3kg of dandelion, uniformly mixing with 10kg of ginger powder, putting the mixture into a beaker, adding 30kg of absolute ethyl alcohol, heating the mixture in a water bath to 40 ℃, dropwise adding 10kg of weak acid electrolyzed water under the stirring condition, and extracting for 30min to obtain the traditional Chinese medicine bactericide; the preparation method of the decomposed animal excrement comprises the following steps: the method comprises the steps of (1) crushing water hyacinth to the water content of 8% to 10mm to obtain water hyacinth crushed materials, uniformly mixing the water hyacinth crushed materials, oyster shell powder and animal manure with the water content of 50%, adding EM microbial inoculum for fermenting and decomposing for 30 days, adding nitrogen fertilizer, phosphate fertilizer and potassium fertilizer, and uniformly mixing to obtain decomposed animal manure;
2) selecting excellent grape saplings, soaking the grape saplings in warm water for 60min, then soaking the grape saplings in 75% alcohol solution for 3min, taking out the grape saplings, placing the grape saplings in the sun, drying the grape saplings in the sun, soaking the grape saplings for 2h by using the embelia meiliana extract, taking out the grape saplings, uniformly dipping plant ash on the roots of the grape saplings, planting the grape saplings at a plant spacing of 85cm between every two adjacent grapes in each row, paving hay around the grape saplings, and spraying a traditional Chinese medicine insecticide on the hay, wherein the traditional Chinese medicine insecticide comprises the following raw materials in parts by weight: 3kg of pyrethrum, 10kg of tobacco leaves, 8kg of allicin, 12kg of gynostemma pentaphylla, 6kg of menthol, 6kg of rhizoma arisaematis, 13kg of golden cypress, 10kg of hawthorn flowers, 12kg of chinaberry seeds and 11kg of rutin; the preparation method of the plukenetia volubilis linneo extract comprises the following steps: airing and drying the plukenetia volubilis linneo until the moisture content is 6%, adding an ethanol solution which is 4 times the mass of the plukenetia volubilis linneo and has the mass fraction of 75%, performing reflux extraction for 60min at the temperature of 70 ℃, cooling to room temperature, filtering to obtain a filtrate, performing rotary evaporation drying on the filtrate until the mass of the plukenetia volubilis linneo is 0.5 times that of the plukenetia volubilis linneo, adding a chitosan solution with the mass fraction of 3%, and uniformly stirring to obtain the plukenetia volubilis linneo extract, wherein the preparation: dissolving 3kg of chitosan in 100kg of acetic acid solution with the mass fraction of 1%, stirring until the solution is clear, filtering, and adjusting the pH value of the filtrate to be neutral to obtain the chitosan solution;
3) after the grapevine sprouts to 20cm, spraying a new high-fat film with the concentration of 10%, thymol and azadirachtin mixed solution to the grapevine leaf surfaces every 10 days, and pruning the grapevine seedlings when the grapevine seedlings grow to 200cm, so that the lengths of the grapevine seedlings are controlled to be 170 cm;
4) after the grape vines are planted, organic liquid fertilizer is dripped to the roots of the grape vines every 15 days, 0.2kg of organic liquid fertilizer is applied to each grape vine, chitin liquid fertilizer and abamectin are dripped to the roots of the grape vines every 10 days after the grapes blossom, rotten animal manure is applied to the roots every 20 days, when the illumination intensity in the morning is more than 4000 lux, weak acid electrolyzed water with the pH value of 6.5 and the effective chlorine concentration of 10mg/L is used for foliage spraying and continuous planting, and the grapes are picked after being ripe, so that the grapes are obtained.
Compared with the grape vine seedlings which are not soaked by the plukenetia volubilis linneo extract, the survival rate of the grape vine seedlings after being soaked by the plukenetia volubilis linneo extract is improved by 48%.
Example 5
A planting method of pollution-free grapes comprises the following steps:
1) selecting a field with a good soil structure, deeply ploughing the soil by 50cm, mixing 1000kg of decomposed animal excrement and 50kg of traditional Chinese medicine bactericide into each mu of the field when ploughing, ridging the planting field along rows, wherein the row spacing is 2m, and erecting a grape vine climbing shelf along the rows, wherein the preparation method of the traditional Chinese medicine bactericide comprises the following steps: crushing 13kg of perilla leaf, 6kg of folium isatidis and 3kg of dandelion, uniformly mixing with 10kg of ginger powder, putting the mixture into a beaker, adding 30kg of absolute ethyl alcohol, heating the mixture in a water bath to 40 ℃, dropwise adding 10kg of weak acid electrolyzed water under the stirring condition, and extracting for 30min to obtain the traditional Chinese medicine bactericide; the preparation method of the decomposed animal excrement comprises the following steps: the method comprises the steps of (1) crushing water hyacinth to the water content of 8% to 10mm to obtain water hyacinth crushed materials, uniformly mixing the water hyacinth crushed materials, oyster shell powder and animal manure with the water content of 50%, adding EM microbial inoculum for fermenting and decomposing for 30 days, adding nitrogen fertilizer, phosphate fertilizer and potassium fertilizer, and uniformly mixing to obtain decomposed animal manure;
2) selecting excellent grape saplings, soaking the grape saplings in warm water for 60min, then soaking the grape saplings in 75% alcohol solution for 3min, taking out the grape saplings, placing the grape saplings in the sun for drying, then soaking the grape saplings for 2h by using the Plukenetia volubilis fruit extract, taking out the grape saplings, uniformly dipping plant ash on the roots of the grape saplings, planting the grape saplings at a plant spacing of 85cm between two adjacent grapes in each row, laying hay around the grape saplings, and spraying a traditional Chinese medicine insecticide on the hay, wherein the preparation method of the traditional Chinese medicine insecticide comprises the following steps of: adding water with the mass 3 times of that of the medicinal materials into 3kg of pyrethrum, 10kg of tobacco leaves, 8kg of allicin, 12kg of gynostemma pentaphylla, 6kg of menthol, 6kg of rhizoma arisaematis, 13kg of golden cypress, 10kg of hawthorn flowers, 12kg of chinaberry seeds and 11kg of rutin respectively, decocting for 1 hour, combining the decoction, and performing rotary evaporation at the temperature of 80 ℃ until the mass is 1 time of the sum of the medicinal materials to obtain the traditional Chinese medicine pesticide; the preparation method of the plukenetia volubilis linneo extract comprises the following steps: airing and drying the plukenetia volubilis linneo until the moisture content is 6%, adding an ethanol solution which is 4 times the mass of the plukenetia volubilis linneo and has the mass fraction of 75%, performing reflux extraction for 60min at the temperature of 70 ℃, cooling to room temperature, filtering to obtain a filtrate, performing rotary evaporation drying on the filtrate until the mass fraction of the filtrate is 0.5 times that of the plukenetia volubilis linneo, adding a chitosan solution with the mass fraction of 3%, and uniformly stirring to obtain the plukenetia volubilis linneo extract, wherein the preparation method of the chitosan solution comprises the following: dissolving 3kg of chitosan in 100kg of acetic acid solution with the mass fraction of 1%, stirring until the solution is clear, filtering, and adjusting the pH value of the filtrate to be neutral to obtain the chitosan solution;
3) after the grapevine sprouts to 20cm, spraying a new high-fat film with the concentration of 10%, thymol and azadirachtin mixed solution to the grapevine leaf surfaces every 10 days, and pruning the grapevine seedlings when the grapevine seedlings grow to 200cm, so that the lengths of the grapevine seedlings are controlled to be 170 cm;
4) after the grape vines are planted, organic liquid fertilizer is dripped to the roots of the grape vines every 15 days, 0.2kg of organic liquid fertilizer is applied to each grape vine, chitin liquid fertilizer and abamectin are dripped to the roots of the grape vines every 10 days after the grapes blossom, rotten animal manure is applied to the roots every 20 days, when the illumination intensity in the morning is more than 4000 lux, weak acid electrolyzed water with the pH value of 6.5 and the effective chlorine concentration of 15mg/L is used for foliage spraying and continuous planting, and the grapes are picked after being ripe, so that the grapes are obtained.
The traditional Chinese medicine pesticide prepared by the embodiment can effectively kill or repel aphids, mites and anthriscus sylvestris.
Example 6
A planting method of pollution-free grapes comprises the following steps:
1) selecting a field with a good soil structure, deeply ploughing the soil by 50cm, mixing 1000kg of decomposed animal excrement and 50kg of traditional Chinese medicine bactericide into each mu of the field when ploughing, ridging the planting field along rows, wherein the row spacing is 2m, and erecting a grape vine climbing shelf along the rows, wherein the preparation method of the traditional Chinese medicine bactericide comprises the following steps: crushing 13kg of perilla leaf, 6kg of folium isatidis and 3kg of dandelion, uniformly mixing with 10kg of ginger powder, putting the mixture into a beaker, adding 30kg of absolute ethyl alcohol, heating the mixture in a water bath to 40 ℃, dropwise adding 10kg of weak acid electrolyzed water under the stirring condition, and extracting for 30min to obtain the traditional Chinese medicine bactericide; the preparation method of the decomposed animal excrement comprises the following steps: the method comprises the steps of (1) crushing water hyacinth to the water content of 8% to 10mm to obtain water hyacinth crushed materials, uniformly mixing the water hyacinth crushed materials, oyster shell powder and animal manure with the water content of 50%, adding EM microbial inoculum for fermenting and decomposing for 30 days, adding nitrogen fertilizer, phosphate fertilizer and potassium fertilizer, and uniformly mixing to obtain decomposed animal manure;
2) selecting excellent grape saplings, soaking the grape saplings in warm water for 60min, then soaking the grape saplings in 75% alcohol solution for 3min, taking out the grape saplings, placing the grape saplings in the sun for drying, then soaking the grape saplings for 2h by using the Plukenetia volubilis fruit extract, taking out the grape saplings, uniformly dipping plant ash on the roots of the grape saplings, planting the grape saplings at a plant spacing of 85cm between two adjacent grapes in each row, laying hay around the grape saplings, and spraying a traditional Chinese medicine insecticide on the hay, wherein the preparation method of the traditional Chinese medicine insecticide comprises the following steps of: adding water with the mass 3 times of that of the medicinal materials into 3kg of pyrethrum, 10kg of tobacco leaves, 8kg of allicin, 12kg of gynostemma pentaphylla, 6kg of menthol, 6kg of rhizoma arisaematis, 13kg of golden cypress, 10kg of hawthorn flowers, 12kg of chinaberry seeds and 11kg of rutin respectively, decocting for 1 hour, combining the decoction, and performing rotary evaporation at the temperature of 80 ℃ until the total mass is 1 time of that of the medicinal materials to obtain the traditional Chinese medicine pesticide; the preparation method of the plukenetia volubilis linneo extract comprises the following steps: airing and drying the plukenetia volubilis linneo until the moisture content is 6%, adding an ethanol solution which is 4 times the mass of the plukenetia volubilis linneo and has the mass fraction of 75%, performing reflux extraction for 60min at the temperature of 70 ℃, cooling to room temperature, filtering to obtain a filtrate, performing rotary evaporation drying on the filtrate until the mass fraction of the filtrate is 0.5 times that of the plukenetia volubilis linneo, adding a chitosan solution with the mass fraction of 3%, and uniformly stirring to obtain the plukenetia volubilis linneo extract, wherein the preparation method of the chitosan solution comprises the following: dissolving 3kg of chitosan in 100kg of acetic acid solution with the mass fraction of 1%, stirring until the solution is clear, filtering, and adjusting the pH value of the filtrate to be neutral to obtain the chitosan solution;
3) after the grapevine sprouts to 20cm, spraying a new high-fat film with the concentration of 10%, thymol and azadirachtin mixed solution to the grapevine leaf surface every 10 days, wherein the mass ratio of the new high-fat film to the thymol to the azadirachtin is 10:2:5, and pruning the grapevine seedlings when the grapevine seedlings grow to 200cm, so that the lengths of the seedlings are controlled to 170 cm;
4) after grape vines are planted, organic liquid fertilizer is dripped to the roots of the grape vines every 15 days, 0.2kg of organic liquid fertilizer is applied to each grape vine, chitin liquid fertilizer and abamectin are dripped to the roots of the grape vines every 10 days after the grapes blossom, rotten animal manure is applied to the roots every 20 days, 100g of the chitin liquid fertilizer, 20g of the abamectin and 2kg of the rotten animal manure are dripped to each grape vine, weak acid electrolyzed water with the pH value of 6.5 and the effective chlorine concentration of 13mg/L is used for foliage spraying every 23 days when the illumination intensity in the morning is greater than 4000 lux, the grape vines are planted continuously, and the grape vines are picked after being ripe.
Comparative example 1
Groups 1-1: the outbreak rates of anthracnose, brown spot, downy mildew and white rot of grapes were counted in the same planting process as in example 6 without adding a traditional Chinese medicine bactericide, and the results are shown in table 1.
Groups 1-2: the outbreak rates of anthracnose, brown spot, downy mildew and white rot of grapes were counted in the same manner as in example 6 except that no new high-fat film, thymol and azadirachtin were sprayed, and the results are shown in table 1.
Groups 1 to 3: the procedure of planting the grape without adding the traditional Chinese medicine bactericide and spraying the new high-fat film, the thymol and the azadirachtin is completely the same as that of the example 6, and the outbreak rates of the anthracnose, the brown spot, the downy mildew and the white rot of the grape are counted, and the results are shown in table 1.
TABLE 1
|
Anthracnose (%)
|
Brown spot (%)
|
Downy mildew (%)
|
White rot (%)
|
Group 1-1
|
7.5
|
17.4
|
14.1
|
9.9
|
Groups 1 to 2
|
7.1
|
24.4
|
18.5
|
11.8
|
Groups 1 to 3
|
11.1
|
22.6
|
21.6
|
16.9
|
Example 6
|
1.2
|
6.7
|
7.5
|
4.1 |
From the results in table 1, it can be seen that the addition of the Chinese medicinal bactericide and the spraying of the new high-fat film, thymol and azadirachtin have better control effects on anthracnose, brown spot, downy mildew and white rot of grapes.
Comparative example 2
Group 2-1: the traditional Chinese medicine insecticide is not added, other planting processes are completely the same as those in example 6, the outbreak rates of grape aphids and mites are counted, and the results are shown in table 2.
Groups 2 to 2: the new high-fat film, thymol and azadirachtin are not sprayed, other planting processes are completely the same as example 6, the outbreak rates of grape aphids and mites are counted, and the results are shown in table 2.
Groups 2 to 3: the planting process is completely the same as that of example 6 except that no traditional Chinese medicine pesticide is added and no new high-fat film, thymol and azadirachtin are sprayed, the outbreak rates of grape aphids and mites are counted, and the results are shown in table 2.
TABLE 2
|
Aphid (%)
|
Mites (%)
|
Group 2-1
|
10.5
|
7.8
|
Group 2-2
|
11.6
|
11.4
|
Groups 2 to 3
|
21.5
|
19.6
|
Example 6
|
2.4
|
3.1 |
As can be seen from the results in Table 2, the addition of the traditional Chinese medicine insecticide and the spraying of the new high-fat film, thymol and azadirachtin can effectively prevent the outbreak of aphids and mite pests.
Comparative example 3
Group 3-1 in step 4, the grapes were foliar-sprayed with distilled water instead of weak acid electrolyzed water, the other planting processes were exactly the same as in example 6, and the outbreak rate of late rot was counted, and the results are shown in table 3.
TABLE 3 incidence of late rot
From the results in Table 3, it can be seen that spraying weak acid electrolyzed water on the leaf surface of grape leaves can greatly reduce the incidence of late rot.
While embodiments of the invention have been disclosed above, it is not intended to be limited to the uses set forth in the specification and examples. It can be applied to all kinds of fields suitable for the present invention. Additional modifications will readily occur to those skilled in the art. Therefore, the invention is not to be limited to the specific details and embodiments shown and described herein, without departing from the general concept defined by the appended claims and their equivalents.