CN102365940A - Botanical sterilization preparation and application thereof for controlling pepper anthracnose - Google Patents
Botanical sterilization preparation and application thereof for controlling pepper anthracnose Download PDFInfo
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- CN102365940A CN102365940A CN2011104003912A CN201110400391A CN102365940A CN 102365940 A CN102365940 A CN 102365940A CN 2011104003912 A CN2011104003912 A CN 2011104003912A CN 201110400391 A CN201110400391 A CN 201110400391A CN 102365940 A CN102365940 A CN 102365940A
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Abstract
The invention discloses a botanical sterilization preparation, which contains the following components in percentage by weight: 0.1-50% of thymol, 5-30% of emulsifier, 0.1-20% of azone, 10-60% of ethanol, and 0.1-84.8% of water. The invention further discloses a preparation method and application of the botanical sterilization preparation to control of pepper anthracnose. Pharmaceutical components of the botanical sterilization preparation based on thymol come from plants or extractives thereof with an insecticidal function; the botanical sterilization preparation not only can be used for effectively controlling the pepper anthracnose but also can be used for promoting growth and development of crops, increasing production, increasing income, improving quality, increasing nutrient utilization rate, controlling injurious insects, avoiding pollution and the like; the botanical sterilization preparation is applied to the production of nuisanceless, green and organic agricultural products, is obvious in application effect, simple in production process, high in market potential, and obvious in economic benefit and social benefit, and has very wide application prospect; and raw materials are easy to obtain.
Description
Technical field
The invention belongs in the agricultural production fruits and vegetables technical field of preventing and curing diseases, be specifically related to a kind of plant resource biocide preparation and the application in the control pepper anthracnose thereof.
Background technology
Pepper anthracnose (Colletotrichum capsici) is that the speciogenesis on capsicum, the pimento is general and endanger serious worldwide disease.In recent years, along with the continuous expansion of pepper planting area, the disease of capsicum trend multiple and that increase the weight of is obvious day by day, and therefore the yield and quality of capsicum receives very big influence.Pepper anthracnose is the regular incidence evil on capsicum produces, and it is general to distribute, especially in the rainy time; Main harm fruit and blade are the main causes that causes capsicum " three fall ", and diseased fruit rate is about 10% usually; Diseased fruit rate reaches 30%~40% when serious, but the underproduction can cause that pepper seedling is dead more than 50%; Leaf really rots, and is all influential to capsicum quality, output.Pepper anthracnose is several kinds of caused fungal diseases in the Deuteromycotina colletotrichum (Colletotrichum Cord).Pepper anthracnose can cause harm Folium Capsici sheet and fruit show the circular spot of water soaking mode brown, verticillate pore on the scab on sick leaf; Sick fruit goes up and is the circular spot of water soaking mode brown, and scab is protruding gradually, forms taupe, black or orange red granule point, is arranged in the concentric wheel stripe spot, and pale red or orange red mucilage are often overflowed in disease portion during high humidity, i.e. conidium.The drying shrinkage attenuation of disease portion is prone to break when dry.Pepper anthracnose can be divided into red anthrax bacteria, black anthracnose bacterium and stain anthrax bacteria according to the disease symptom.
Present on the means of control pepper anthracnose, mainly still chemical control, chemical control is to use bactericide to prevent and treat pepper anthracnose.Efficient, quick-acting, easy to use, economic benefit advantages of higher that bactericide has, but have only protective effect, do not have treatment and root out effect, and the lasting period to be short, application times is many, and germ has produced pesticide resistance to it; Long-time unreasonable use chemical pesticide control pepper anthracnose causes pathogen to develop immunity to drugs gradually, and control efficiency reduces, and can increase residue of pesticide in the fruit, and contaminated environment increases production cost.Chemical pesticide still is difficult to solve effectively to people and animals' toxic and side effect and residue problem so far.
Along with improve constantly with various countries the pay attention to day by day to protection environment for human survival of the world today to crops quality and quantity demand; And the deepening continuously of sustainable agriculture and lasting plant protection idea; The production of people's growing interest environmental problem and pollution-free food; Developing out efficient, environmentally friendly botanical biological pesticide control pepper anthracnose is the focus of studying at present, also is developing tendency in future.Through the biopesticide of processing after the improvement of vegetable source natural compound, not only control efficiency is good, also to fruit, flower, the Ye Douwu harm of plant, to the person poultry safety, has efficient noresidue, no poisoning, advantages such as the non-resistant property of medicine; In the control disease, can effectively improve quality of agricultural product simultaneously.
Summary of the invention
Technical problem to be solved by this invention provides a kind of plant resource biocide preparation based on thymol, and said preparation is a kind of friendly preparation of many effects novel environmental that integrates antibiotic, nutrition, improves quality.
The technical problem that the present invention also will solve provides the preparation method of above-mentioned plant resource biocide preparation.
The technical problem that the present invention will solve at last provides the application of above-mentioned plant resource biocide preparation in the control pepper anthracnose.
For solving the problems of the technologies described above, the technical scheme that the present invention adopts is following:
A kind of plant resource biocide preparation, it comprises the component of following percetage by weight: thymol 0.1~50%, emulsifier 5~30%, azone 0.1~20%, ethanol 10~60%, water 0.1~84.8%.
Above-mentioned plant resource biocide preparation preferably comprises the component of following percetage by weight: thymol 0.5~5%, emulsifier 10~20%, azone 1~2%, ethanol 20~40%, water 33~68%.
Wherein, described emulsifier is one or more the mixture in phenethyl phenol polyethenoxy ether, phenethyl phenol polyoxyethylene poly-oxygen propylene aether, aralkyl phenol polyethenoxy ether phosphate, APES phosphate, alkyl naphthalene sulfonic acid calcium, castor oil ethylene oxide adduct, aralkyl phenol polyethenoxy ether, AEO, alkyl phenol formaldehyde resin polyoxyethylene ether and the dialkyl benzene sulfonic acids calcium.
Above-mentioned thymol can carry out composite with carbendazim, Tebuconazole.
The preparation method of above-mentioned plant resource biocide preparation; Press formula ratio and prepare raw material thymol, emulsifier, azone, second alcohol and water; Thymol added mix the formation solution A in the part ethanol; Again emulsifier and azone are got with remaining dissolve with ethanol formation solution B, then solution A, solution B and water being mixed promptly.
Wherein, ethanol uses (being above-mentioned part ethanol and above-mentioned residue ethanol) at twice, the abundant dissolving of each used in amounts raw material that assurance is dissolved, and twice consumption sum is the ethanol raw material total amount of formula ratio.
The application of above-mentioned plant resource biocide preparation in promoting chili growth.
The application of above-mentioned plant resource biocide preparation in the control pepper anthracnose.
Beneficial effect: its effective component of plant resource biocide preparation based on thymol of the present invention derives from plant or its extract with insecticidal function; Can not only effectively prevent and treat pepper anthracnose; Have simultaneously promote crop growth, increasing both production and income, improve quality, improve plant recovery of nutrient, pest control, multi-efficiency such as pollution-free, be applicable to nuisanceless, green and organic farm products production.The said preparation effect significantly, raw material are easy to get, and production technology is simple, market potential is big, economic benefit and social benefit are obvious, has the wide application prospect of ten minutes.
Embodiment
According to following embodiment, can understand the present invention better.Yet, those skilled in the art will readily understand that embodiment is described only to be used to explain the present invention, and the present invention that should also can not limit in claims to be described in detail.
Embodiment 1:
A kind of plant resource biocide preparation, it comprises the component of following percetage by weight: thymol 2%, emulsifier (emulsifier is the phenethyl phenol polyethenoxy ether) 20%, azone 2%, ethanol 30%, water 46%.
The preparation method of above-mentioned plant resource biocide preparation; Press formula ratio and prepare raw material thymol, emulsifier, azone, second alcohol and water; Mix the formation solution A in the ethanol with thymol adding 1/4 amount; Dissolve with ethanol with emulsifier and azone usefulness residue 3/4 amount forms solution B again, solution A, solution B and water is mixed promptly to get then.
Embodiment 2:
A kind of plant resource biocide preparation; It comprises the component of following percetage by weight: thymol 5%; Emulsifier (emulsifier is that emulsifier is aralkyl phenol polyethenoxy ether phosphate and APES phosphate by weight 1: 1 mixture) 20%; Azone 4%, ethanol 40%, water 31%.
The preparation method of above-mentioned plant resource biocide preparation; Press formula ratio and prepare raw material thymol, emulsifier, azone, second alcohol and water; Mix the formation solution A in the ethanol with thymol adding 1/2 amount; Dissolve with ethanol with emulsifier and azone usefulness residue 1/2 amount forms solution B again, solution A, solution B and water is mixed promptly to get then.
Embodiment 3:
A kind of plant resource biocide preparation, it comprises the component of following percetage by weight: thymol 0.5%, emulsifier (emulsifier is the APES phosphate) 10%, azone 2%, ethanol 20%, water 67.5%.
The preparation method is with embodiment 2.
Embodiment 4:
A kind of plant resource biocide preparation; It comprises the component of following percetage by weight: thymol 0.1%, emulsifier (emulsifier is alkyl naphthalene sulfonic acid calcium and castor oil ethylene oxide adduct by weight 1: 2 mixture) 30%, azone 0.1%; Ethanol 60%, water 9.8%.
The preparation method is with embodiment 2.
Embodiment 5:
A kind of plant resource biocide preparation; It comprises the component of following percetage by weight: thymol 50%; Emulsifier (emulsifier is aralkyl phenol polyethenoxy ether, AEO and alkyl phenol formaldehyde resin polyoxyethylene ether by weight 1: 2: 1 mixture) 5%; Azone 20%, ethanol 10%, water 15%.
The preparation method is with embodiment 2.
Embodiment 6: based on the plant resource biocide preparation of thymol to the promotion of plant growth with to the improvement effect of quality.
Formula I: 1/2Hoagland nutrient solution.
The plant resource biocide preparation of the thymol that formula I I: embodiment 1 makes.
Seed is with the H of 0.3% (w/w)
2O
2After the sterilization, clean, select the culture dish (diameter 15em) that homogeneous, the normal seed of form place shop 3 metafiltration paper then, train seedling with distilled water.5d after waiting to emerge chooses the consistent material of growing way it is transferred in the plastic basin alms bowl, behind the seedling age 20d, sprays with containing different disposal liquid respectively, and each handles repetition 3 times.Treatment fluid is every to spray once at a distance from 5d, samples behind the 60d, measures and respectively handles green vegetables relevant physiological biochemical indicator.(annotate: contrast CK is a clear water)
Assay method:
Fresh weight is measured: after the off-test, every basin is the consistent seedling of 3 upgrowth situations of picking at random, measures its fresh weight with the clean back of blotting of deionized water then.
Protein content determination: the method with reference to Bradford is measured.(Bradford?M?M.A?rapid?and?sensitive?method?for?the?quantitation?of?microgram?quantities?of?protein?using?the?principle?of?protein-dye?binding.Ann.Biochem,1976,72:248-254.)
Content of nitrite is measured: with reference to standard GB/T 15401-1994 " mensuration of fruit and vegetable and goods thereof---nitrite and nitrate content "; Get green vegetables sample 2~3g; Be ground to the homogenate shape, under weak basic condition, from sample, extract nitrite ion (NO with hot water
2 -), with potassium ferrocyanide and zinc acetate protein precipitation, cross and filter transparent colourless solution, add sulfanilamide (SN) and naphthodiamide hydrochloride again, measure the absorbance of the red complex that generates at wavelength 538nm place, original nitrite ion (NO in the calculation sample
2 -) content.
Vc assay: get about testing sample 2g, add a small amount of 2% hydrochloric acid, in tissue mashing machine, process homogenate; Move in the 100ml volumetric flask, to scale, absorbent cotton filters with 1% oxalic acid constant volume; Get 10ml filtrating; With demarcated 2,6-dichloroindophenol solution titration determination Vc content carries out with reference to the method for Gao Peng.(high friend. the agrochemistry common analysis. Xi'an: Shaanxi science tech publishing house, 1980)
Soluble sugar content is measured: soluble sugar is measured and is adopted the anthrone colorimetric method, carries out with reference to the method for Zou Qi.(Zou Qi. plant physiology experiment instructs. Beijing: Chinese agriculture publishing house, 2000.)
. table 1 different disposal is to the influence of capsicum biomass and quality
Can know by table 1; Capsicum is handled back fresh weight, output, plant height, protein content, Vc content and soluble sugar content in formula I and compares respectively according to having increased by 13.5%, 25.5%, 17.8%, 23.5%, 14.2% and 39.9%, and the content of nitrite comparison is according to having increased by 5.1%; Formula I I handles back fresh weight, output, plant height, protein content, Vc content and soluble sugar content and compares respectively according to having increased by 25.6%, 40.3%, 24.4%, 44.2%, 40.8% and 54.1%, and the content of nitrite comparison is according to having reduced by 8.3%.
Can know on having, with formula I I handle to capsicum biomass and quality to improve effect best.
Embodiment 7: based on the plant resource biocide preparation of thymol at preventive and therapeutic effect to pepper anthracnose.
Formula I: 70% thiophanate methyl wetting powder.
The plant resource biocide preparation prescription of the thymol of formula I I: embodiment 1.
Choose three processing of morbidity field piece design: handle 1: contrast (contrast CK is a clear water); Handle 2: formula I; Handle 3: formula I I.Each handles repetition 3 times, and treatment fluid is every to spray once at a distance from 5d, carries out statistical analysis after spraying 3 times.
Assay method:
The mensuration of disease index and control efficiency: with reference to Dou Rui flitch method.(Dou Rui wood .3 plants endogenetic bacterium is to the control efficiency of graw mold of tomato. the Henan agricultural science, and 2010,4:77-79.)
Can know that by table 2 on capsicum, formula I is 54.76% to the preventive effect of pepper anthracnose; Formula I I is 85.05% to the preventive effect of pepper anthracnose.
By on can know, handle best to the control efficiency of pepper anthracnose with formula I I.
Table 2 different disposal is to the pepper anthracnose control efficiency
Claims (5)
1. a plant resource biocide preparation is characterized in that, it comprises the component of following percetage by weight: thymol 0.1~50%, emulsifier 5~30%, azone 0.1~20%, ethanol 10~60%, water 0.1~84.8%.
2. plant resource biocide preparation according to claim 1; It is characterized in that described emulsifier is one or more the mixture in phenethyl phenol polyethenoxy ether, phenethyl phenol polyoxyethylene poly-oxygen propylene aether, aralkyl phenol polyethenoxy ether phosphate, APES phosphate, alkyl naphthalene sulfonic acid calcium, castor oil ethylene oxide adduct, aralkyl phenol polyethenoxy ether, AEO, alkyl phenol formaldehyde resin polyoxyethylene ether and the dialkyl benzene sulfonic acids calcium.
3. the preparation method of the described plant resource biocide preparation of claim 1; It is characterized in that; Press formula ratio and prepare raw material thymol, emulsifier, azone, second alcohol and water; Thymol added mix the formation solution A in the part ethanol, again emulsifier and azone are formed solution B with the residue dissolve with ethanol, solution A, solution B and water are mixed promptly get then.
4. the application of the described plant resource biocide preparation of claim 1 in promoting chili growth.
5. the application of the described plant resource biocide preparation of claim 1 in the control pepper anthracnose.
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102365939A (en) * | 2011-12-06 | 2012-03-07 | 江苏省农业科学院 | Botanical sterilization preparation and application thereof for controlling bitter rot or anthracnose of grape |
CN109258637A (en) * | 2018-10-17 | 2019-01-25 | 四川省兰月科技有限公司 | Sterilize the composition pesticide of control shoot |
Citations (2)
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JP2001316214A (en) * | 2000-05-11 | 2001-11-13 | Sadayoshi Usuda | Natural drug against plant disease |
CN1357229A (en) * | 2000-12-13 | 2002-07-10 | 刘岱 | Application, production and processing of elsholtzia herb as natural plant pest and disease preventing and eliminating agent |
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Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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JP2001316214A (en) * | 2000-05-11 | 2001-11-13 | Sadayoshi Usuda | Natural drug against plant disease |
CN1357229A (en) * | 2000-12-13 | 2002-07-10 | 刘岱 | Application, production and processing of elsholtzia herb as natural plant pest and disease preventing and eliminating agent |
Non-Patent Citations (1)
Title |
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张静等: "《百里香酚抑菌活性初探》", 《中国农学通报》 * |
Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102365939A (en) * | 2011-12-06 | 2012-03-07 | 江苏省农业科学院 | Botanical sterilization preparation and application thereof for controlling bitter rot or anthracnose of grape |
CN102365939B (en) * | 2011-12-06 | 2013-04-17 | 江苏省农业科学院 | Botanical sterilization preparation and application thereof for controlling anthracnose of grape |
CN109258637A (en) * | 2018-10-17 | 2019-01-25 | 四川省兰月科技有限公司 | Sterilize the composition pesticide of control shoot |
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