CN105028481A - Biological agent for preventing and treating tobacco mosaic virus as well as preparation method and application of biological agent - Google Patents
Biological agent for preventing and treating tobacco mosaic virus as well as preparation method and application of biological agent Download PDFInfo
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- CN105028481A CN105028481A CN201510449021.6A CN201510449021A CN105028481A CN 105028481 A CN105028481 A CN 105028481A CN 201510449021 A CN201510449021 A CN 201510449021A CN 105028481 A CN105028481 A CN 105028481A
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- mosaic virus
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Abstract
The invention relates to a biological agent for preventing and treating tobacco mosaic virus as well as a preparation method and application of the biological agent, and aims to solve the technical problems that the tobacco mosaic virus is hard to prevent and treat, high in preventing and treating cost and high in possibility of environment pollution. The biological agent consists of a composition I and a composition II according to a volume ratio of (10-300):1, wherein the composition I is mineral oil, and the composition II comprises the following materials in percentage by weight: 1-45% of eugenol, 2-25% of an emulsifying agent, 0.1-15% of azone, 10-60% of alcohol and 1-85% of water. The multi-effect novel environment-friendly preparation provided by the invention integrates the functions of resisting bacteria, controlling and repairing, and is good in tobacco mosaic virus preventing and treating effects, free of drug resistance, and very wide in application prospect.
Description
Technical field
The invention belongs to crop disease prevention in agricultural production to cure the disease technical field, be specifically related to the biologic product and the preparation method and application that prevent and treat tobacco mosaic virus disease.
Background technology
China's tobacco planting area reaches more than 2,000 ten thousand mu, in recent years, along with the expansion of tobacco planting area, causes tobacco mosaic virus disease to be expanded year by year.Tobacco mosaic virus (Tobaccomosaicvirus; TMV), be a kind of single strand RNA virus, special infection tobacco and other plants of Solanaceae, can make these infected blades seem mottled stained.After tobacco plant is caught an illness, young leaflet tablet lateral vein and offshoot tissue are translucent shape, i.e. bright arteries and veins, and vein both sides mesophyll tissue is gradually in light green.Virus is bred in a large number in leaf tissue, and make part mesophyll cell increase or increase, occur that blade thin and thick is irregular, color is yellowish green alternate, in floral leaf shape.The mottled degree of rear floral leaf strengthens, and existing large area dark brown necrotic plaque, middle and lower part Lao Ye more so, the heavy leaf-shrinkage of morbidity, deformity, distortion.Tobacco mosaic virus disease is distributed widely in Ge Yan district of China, it is tobacco main diseases virus disease, wherein the ground such as Heilungkiang, Jilin, Liaoning, Shandong, Henan, Anhui, Hubei, Sichuan, Chongqing, Guizhou, Yunnan, Fujian, Guangdong, Taiwan is injured heavier, and the loss endangered tobacco is caused can reach about 50%.
Spread to effectively control the viroses of plant; that fundamentally carries out preventing virus diseases reduces the loss; researcher have employed various measure; comprising the control etc. of selecting, passing virus mediator of the attenuated strain system cross protection between crop rotation, seed detoxification, virus, disease-resistant variety, the disease-resistant research of genetically modified plants in recent years there has also been new progress.But at present mainly still chemical pesticide is relied on to the control of virus disease, as: mushroom proteoglycan, octicin solution, ABOB copper acetate (poison can gram, virus gram absolutely, poison to the greatest extent), ribavirin copper zinc, nonyl phenol sulfonic acid copper, bacterium ABOB, mixed ester acid copper etc.; But, use chemical pesticide not only can produce residue of pesticide, and also increasing gradually along with the pesticide resistance of virus disease that uses year by year of chemical pesticide, vegetable grower can only strengthen the usage amount of agricultural chemicals for this reason, if but excessively strengthen consumption, not only can affect plant growth, increase fruit Pesticide Residues, suppress beneficial microbe growth in soil, and pollute the environment, increase production cost.
Under the prerequisite of current promotion pollution-free food and environmental protection energetically, one of key content of person's work that the integrated control strengthening plant disease and the use reducing chemical pesticide have become plant protection work.And develop a focus of efficient, the environmentally friendly biological agent person that also becomes plant protection work research.
Summary of the invention
The object of this invention is to provide biologic product and the preparation method and application of control tobacco mosaic virus disease, described biologic product integrates antibacterial, controls, repairs, and is a kind of environmentally friendly biologic product.
The technical solution used in the present invention is as follows:
The biologic product of control tobacco mosaic virus disease, described biologic product is made up of component I mineral oil and component I I, and both volume ratios are 10 ~ 300:1; Wherein, the weight percent of component I I is composed as follows: eugenol 1 ~ 45%, emulsifier 2 ~ 25%, azone 0.1 ~ 15%, alcohol 10 ~ 60%, water 1 ~ 85%.
Preferably, the weight percent of described component I I is composed as follows: eugenol 5 ~ 25%, emulsifier 2 ~ 25%, azone 0.1 ~ 5%, alcohol 20 ~ 60%, water 20 ~ 60%.
More preferred, the weight percent of described component I I is composed as follows: eugenol 10 ~ 20%, emulsifier 15 ~ 25%, azone 2 ~ 3%, alcohol 40 ~ 50%, water 7 ~ 28%.
Preferably, the volume ratio of component I and component I I is 50 ~ 200:1.
Alcohol can adopt volumetric concentration to be the alcohol of 92-99%, and the amount added converts accordingly.
Preferably, described mineral oil is machine oil or diesel oil.
Preferably, described emulsifier is at least one in phenethyl phenol polyethenoxy ether, phenethyl phenol polyethenoxy polyethenoxy ether, aralkyl phenol polyethenoxy ether phosphate, alkylphenol polyoxyethylene phosphate, alkyl naphthalene sulfonic acid calcium, castor oil ethylene oxide adduct, aralkyl phenol polyethenoxy ether, alkyl phenol formaldehyde resin polyoxyethylene ether or dialkyl benzene sulfonic acids calcium.
The present invention still further provides a kind of preparation method of biologic product of above-mentioned control tobacco mosaic virus disease, preferably but be not limited to carry out as follows: first prepare the solution A that eugenol is dissolved in alcohol, prepare the solution B that emulsifier and azone are dissolved in alcohol again, add water after A, B mixing and mix and obtain component I I; Proportionally namely blending ingredients I and component I I obtains the biologic product of described control tobacco mosaic virus disease.The biologic product stable performance obtained after mixing, constituent is not easily oxidized, each component energy Synergistic, and it is long to use rear drug effect, long shelf-life during shelf time.
Preferably, the ratio of alcohol for the preparation of solution A and preparation solution B is 40 ~ 70:30 ~ 60.
Biologic product prepared by the present invention is aqueous emulsion.
Biologic product of the present invention has extraordinary application in control tobacco mosaic virus disease.
The invention provides a kind of mineral oil to combine with eugenol the propagation preventing and treating tobacco mosaic virus disease and the biologic product spread.Mineral oil component in preparation, be different from prior art the function be used for as solvent, in the present invention, utilize the oil film that the mineral oil after spraying is formed at plant surface, stop the children of insect, nymph stops falling, the effect can played expelling parasite and suppress germ to encroach on, thus decrease the use of insecticide and bactericide; Another main component eugenol derives from plant resource, and experiment shows there is good control effect to the virus disease of various crop.Mineral oil combines with eugenol by the present invention, deep research is carried out to both usage ratios simultaneously, make composite biologic product effectively cut off insect on the one hand to the propagation of virus disease, effectively can repair the loss that virus disease is brought on the other hand, make the plant recovery more than 70% of catching an illness.Concrete mechanism is as follows: eugenol can improve a series of defensive ferment relevant with disease resistance in tobacco body under the synergy of the composition such as azone, ethanol, excite in plant body and produce defense response, and facilitate the synthesis of protective plant protecting agent, quinones substance, tannin and lignin etc. and disease resistance related substance, improve the ability of tobacco plant Scavenger of ROS, reduce the metabolic dysequilibrium that virus infection causes, improve the resistivity to virus infection.
Described biologic product preventive effect is high, instant effect, drug effect phase are long, is used by 3 ~ 5 times, can prevention and control whole breeding times tobacco mosaic virus disease generation and spread; Can not remain crop because product belongs to micro-poison simultaneously, therefore have efficient noresidue advantage, spray this medicament and can not produce medicine spot (without poisoning), whole breeding time, 3 ~ 5 uses can not be developed immunity to drugs; Easy to use, economic benefit and social benefit obviously, have very wide application prospect.
Described biologic product raw material are easy to get, and production technology is simple, are convenient to promote.
Compared with prior art, tool has the following advantages in the present invention:
The invention provides a kind of friendly preparation of many effects novel environmental integrating antibacterial, control, repair,
Prevent and treat that virus disease is effective, without pesticide resistance, and drug effect is long, storage endurance, has very wide application prospect.
Embodiment
According to following embodiment, the present invention may be better understood.But those skilled in the art will readily understand, the content described by embodiment only for illustration of the present invention, and should can not limit the present invention described in detail in claims yet.
embodiment 1
The biologic product of control tobacco mosaic virus disease, be made up of component I mineral oil and component I I, both volume ratios are 150:1.
Wherein, the weight percent of component I I is composed as follows: eugenol 10%, emulsifier 22%, azone 2.5%, alcohol 50%, and surplus is water.
Emulsifier is alkylphenol polyoxyethylene phosphate.
The preparation method of described biologic product is as follows:
First eugenol is added in part alcohol and mix formation solution A, then the alcohol of emulsifier and azone surplus is dissolved formation solution B, then A and B is mixed, finally carry out constant volume with pure water, obtain component I I.Two kinds of components are mixed in proportion and obtains described biologic product.Wherein, the volume ratio for the preparation of the alcohol of solution A and solution B is 4:6.
embodiment 2
The biologic product of control tobacco mosaic virus disease, be made up of component I mineral oil and component I I, both volume ratios are 200:1.
Wherein, the weight percent of component I I is composed as follows: eugenol 20%, emulsifier 25%, azone 3%, alcohol 45%, and surplus is water.
Emulsifier is phenethyl phenol polyethenoxy polyethenoxy ether and aralkyl phenol polyethenoxy ether phosphate is the mixture of 1:1 by weight.
The preparation method of described biologic product is with embodiment 1.
embodiment 3
The biologic product of control tobacco mosaic virus disease, be made up of component I mineral oil and component I I, both volume ratios are 100:1.
Wherein, the weight percent of component I I is composed as follows: eugenol 15%, emulsifier 15%, azone 2%, alcohol 40%, and surplus is water.
Emulsifier is aralkyl phenol polyethenoxy ether phosphate and the alkylphenol polyoxyethylene phosphate mixture by weight 1:1.
The preparation method of described biologic product is with embodiment 1.
effect example
Relatively descend the performance of series preparation in the preventing and treating of tobacco mosaic virus disease.
Formula I: 20% glucocyamine copper wetting powder.
Formula I I:20% eugenol aqueous emulsion.
Formula I II: the biologic product that embodiment 2 prepares.
(1) control experiment
Tobacco seed uses the H of 0.3% (w/w) respectively
2o
2after sterilization, clean with distilled water, then select homogeneous, that the normal seed of form is placed in paving 3 metafiltration paper culture dish (diameter 15cm), carry out training seedling.5d after emerging, chooses the consistent material of growing way and transfers them in plastic basin alms bowl, and after seedling age 20d, the consistent seedling of growth selection is for subsequent use.Get appropriate tobacco disease leaf, with 0.01mol/L(pH7.0) PBS buffer solution grinding pulping, by frictional inoculation to tobacco leaf.Process respectively: CK(contrast CK for spray clear water), formula I, formula I I and formula I II, each process 100 strain, each process repeats 3 times.Treatment fluid sprays once every 5d, connects after spraying 2 times and samples.Prevention experiment
Field experiment: choose tobacco experiment 3, plot respectively
,each field arranges 4 process: CK(clear water), formula I, formula I I and formula I II.It is spray after the solution of 333mg/kg that preparation water when spraying of above-mentioned formula I, II and III is mixed with drug concentration again.Each process about 1000 strain, each process repeats 3 times.After tobacco field planting, about 15d starts to look into disease index, starts to spray treatment fluid simultaneously, and treatment fluid sprays once every 5d, and continuous spraying 3 times, Application is according to the routine operation of those skilled in the art.
Assay method
The mensuration of disease index and control efficiency is with reference to Dou Rui flitch method (referring to " 3 plants endogenetic bacteriums are to the control efficiency of graw mold of tomato ", Henan Agricultural Sciences, 2010,4:77-79.).
The measurement result of control experiment and prevention experiment is respectively in table 1, table 2.
The different biologic product of table 1 is to the control efficiency of tobacco mosaic virus disease
As shown in Table 1, when not preventing virus disease, the preventive effect that virus disease prevented and treated by three kinds of biologic products is widely different.When tobacco plants is applied, formula I is 48.21% to the preventive effect of virus disease; Formula I I is 64.27% to the preventive effect of virus disease; Formula I II is 78.53% to the preventive effect of virus disease.The control efficiency of biologic product provided by the invention to tobacco mosaic virus disease is best, reaches more than 70% to the control efficiency of virus disease, is far longer than formula I and formula I I.
The different biologic product of table 2 is to the preventive effect of tobacco mosaic virus disease
As shown in Table 2, by primary prevention, the development of virus disease effectively can be controlled.Specific in the application of tobacco plants, formula I is 52.36% to the preventive effect of virus disease; Formula I I is 72.17% to the preventive effect of virus disease; Formula I II is 82.26% to the preventive effect of virus disease.Visible, the control efficiency of biologic product of the present invention to tobacco mosaic virus disease is best, reaches more than 80% to the preventive effect of virus disease, is far longer than formula I and formula I I.
The present invention is applicable to all kinds of tobacco bred, suitable to the result for the treatment of of tobacco mosaic virus disease.
Claims (9)
1. prevent and treat the biologic product of tobacco mosaic virus disease, it is characterized in that, described biologic product is made up of component I mineral oil and component I I, and both volume ratios are 10 ~ 300:1; Wherein, the weight percent of component I I is composed as follows: eugenol 1 ~ 45%, emulsifier 2 ~ 25%, azone 0.1 ~ 15%, alcohol 10 ~ 60%, water 1 ~ 85%.
2. the biologic product of control tobacco mosaic virus disease as claimed in claim 1, it is characterized in that, the weight percent of described component I I is composed as follows: eugenol 5 ~ 25%, emulsifier 2 ~ 25%, azone 0.1 ~ 5%, alcohol 20 ~ 60%, water 20 ~ 60%.
3. the biologic product of control tobacco mosaic virus disease as claimed in claim 2, it is characterized in that, the weight percent of described component I I is composed as follows: eugenol 10 ~ 20%, emulsifier 15 ~ 25%, azone 2 ~ 3%, alcohol 40 ~ 50%, water 7 ~ 28%.
4. the biologic product of the control tobacco mosaic virus disease as described in as arbitrary in claim 1-3, it is characterized in that, the volume ratio of component I and component I I is 50 ~ 200:1.
5. the biologic product of control tobacco mosaic virus disease as claimed in claim 4, it is characterized in that, described mineral oil is machine oil or diesel oil.
6. the biologic product of control tobacco mosaic virus disease as claimed in claim 4, it is characterized in that, described emulsifier is at least one in phenethyl phenol polyethenoxy ether, phenethyl phenol polyethenoxy polyethenoxy ether, aralkyl phenol polyethenoxy ether phosphate, alkylphenol polyoxyethylene phosphate, alkyl naphthalene sulfonic acid calcium, castor oil ethylene oxide adduct, aralkyl phenol polyethenoxy ether, alkyl phenol formaldehyde resin polyoxyethylene ether, dialkyl benzene sulfonic acids calcium.
7. the preparation method of the biologic product of the arbitrary described control tobacco mosaic virus disease of claim 1-6, it is characterized in that, first prepare the solution A that eugenol is dissolved in alcohol, then prepare the solution B that emulsifier and azone are dissolved in alcohol, add water after A, B mixing and mix and obtain component I I; Proportionally namely blending ingredients I and component I I obtains the biologic product of described control tobacco mosaic virus disease.
8. the preparation method of the biologic product of control tobacco mosaic virus disease as claimed in claim 7, is characterized in that, is 40 ~ 70:30 ~ 60 for the preparation of solution A and the ratio of the alcohol of preparation solution B.
9. the application of the biologic product of the arbitrary described control tobacco mosaic virus disease of claim 1-6.
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Cited By (3)
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| CN106962349A (en) * | 2017-04-17 | 2017-07-21 | 广西南宁益土生物科技有限责任公司 | A kind of antiviral composition containing eugenol and lentinan |
| CN110857438A (en) * | 2018-08-20 | 2020-03-03 | 中国烟草总公司黑龙江省公司牡丹江烟草科学研究所 | Tobacco mosaic virus gene fragment for efficiently generating siRNA, attenuated vaccine, preparation method and application thereof |
| CN113057168A (en) * | 2021-03-22 | 2021-07-02 | 云南中烟工业有限责任公司 | Seed disinfection preparation, preparation method, use method and application thereof |
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| CN113057168A (en) * | 2021-03-22 | 2021-07-02 | 云南中烟工业有限责任公司 | Seed disinfection preparation, preparation method, use method and application thereof |
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Application publication date: 20151111 |