CN102293205A - Osthole and ningnanmycin compound bactericide - Google Patents
Osthole and ningnanmycin compound bactericide Download PDFInfo
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- CN102293205A CN102293205A CN2011101398262A CN201110139826A CN102293205A CN 102293205 A CN102293205 A CN 102293205A CN 2011101398262 A CN2011101398262 A CN 2011101398262A CN 201110139826 A CN201110139826 A CN 201110139826A CN 102293205 A CN102293205 A CN 102293205A
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- osthole
- ningnanmycin
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- powdery mildew
- sheath blight
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Abstract
The invention belongs to the technical field of pesticide, and relates to an osthole and ningnanmycin compound bactericide used for preventing and controlling powdery mildew of strawberry, grape and tomato and rice sheath blight. Active ingredients of the bactericide are osthole and ningnanmycin, and the weight ratio of the osthole to the ningnanmycin is (1:10)-(10:1). The compound bactericide provided by the invention has the advantages that: 1, two bactericides are combined for use, thus bactericidal spectrum is expanded; 2, usage of the osthole is relatively reduced after formulation compared with single dosage, and effective dependence to plant resource is reduced; 3, the compound bactericide provided by the invention is applicable to prevention and control of the powdery mildew and sheath blight, and selection pressure of chemical agents used for preventing and controlling the powdery mildew and sheath blight is reduced; and 4, application dosage is reduced, application frequency is reduced, use cost is reduced, and the advantages of high efficiency, quick acting and long lasting period are embodied.
Description
Technical field
The invention belongs to technical field of pesticide, relate to a kind of Osthole and Ningnanmycin composite bactericide that is used to prevent and treat powdery mildew and the rice sheath blight disease of strawberry, grape, tomato.
Background technology
At present, control crop powdery mildew and the employed chemical bactericide of banded sclerotial blight such as triazolone, nitrile bacterium azoles, thiophanate-methyl, carbendazim, polyoxin etc. have produced pesticide resistance to a certain degree.It is reported that the powdery mildew of strawberry bacterium reaches more than 50% the pesticide resistance of triazolone is the highest.
Coumarin kind compound Osthole (osthol) chemical name is 8-(3-methyl-2-butene base) methyl ether San shape ketone, it is Main Ingredients and Appearance in the umbellate form flower section plant frutus cnidii, also be root, the rhizome of Imperatoria ostruthium, circle Radix Angelicae Sinensis, Angelica pubescens, bolt wing celery, the active component that extracts in the acrial part of the anistree Chinese wampee root skin of rutaceae, single leaf rue etc. is the wild medical material of China's wide material sources.Medically have anticancer, antimutagenesis, the killing pests and suppressing bacteria report has been arranged on agricultural.
Ningnanmycin, other Chineses: bacterium gram poison gram english common name: ningnanmycin, chemical name: 1-(4-flesh glutamine-L-silk amide-4-deoxidation-β-D-glucopyranose aldehyde acid amides) cytimidine, molecular formula C16H23O8N7.This antibiotic is a kind of low toxicity, low-residual, nothing " three cause " and accumulation problems, novel pesticide free from environmental pollution.Virus disease for various crop such as tomato, capsicum, melon, paddy rice, wheat, vegetables, fruit tree, beans, and can prevent and treat bacterial diseases such as fungal diseases such as powdery mildew, root rot, stem rot, damping off, eqpidemic disease, climing rot, anthracnose, paddy rice culm rot and bacterial blight of rice, soft rot of cabbage, solanaceous crops bacterial wilt, also can prevent and treat diseases such as banana bunchy top disease, floral leaf heart rot, pepper mosaic disease, pawpaw ring spot, genseng damping off and stem rot.Not only its fungicidal spectrum is narrower as single agent, and long-term use can make disease develop immunity to drugs, thereby usage amount increases, the drug cost height.
Summary of the invention
Technical problem to be solved by this invention is, provide a kind of fungicidal spectrum wide, to crop powdery mildew and effective Osthole and the Ningnanmycin composite bactericide of rice sheath blight disease controlling.
Osthole of the present invention and Ningnanmycin composite bactericide, its active ingredient are Osthole and Ningnanmycin, and the weight ratio of Osthole and Ningnanmycin is 1: 10～10: 1 in its composition.
Described Osthole and Ningnanmycin composite bactericide are a kind of in the microemulsion that formed by active ingredient and auxiliary agent and carrier combinations, aqueous emulsion, water dispersible granules, the wetting powder.
Advantage of the present invention is:
1. being used in combination of two kinds of bactericide enlarged fungicidal spectrum.
2. the consumption that has reduced Osthole is compared with single agent in composite back, reduces the effective dependence to plant resources.
3. the present invention is applicable to control crop powdery mildew and banded sclerotial blight, can reduce control crop powdery mildew and the banded sclerotial blight selection pressure to chemical agent.
4. the reduction formulation rate reduces application times, reduces use cost, has advantage efficient, quick-acting, that the lasting period is long.
Embodiment
Below the proportion with synergistic effect of Osthole of the present invention and Ningnanmycin composite bactericide and controlling object and composite specific embodiment are elaborated; these contents just are used for explanation; and do not mean that protection scope of the present invention limited that wherein said active ingredient is all in its active ingredient.
One, Osthole and Ningnanmycin composite bactericide lab screening
For trying bacterium and host crop: with the powdery mildew of strawberry bacterium serves as for the examination bacterium, and kind " Feng Xiang " is for trying host crop.
Reagent agent: 10% Osthole (Hubei Tian Hui bio tech ltd provides) is dissolved in 95% ethanol, and it is standby to make the 10000ug/ml mother liquor; Ningnanmycin (Seoul Heilongjiang Qianger biochemical technology development Co., Ltd) is dissolved in sterile water, and it is standby to make the 10000ug/ml mother liquor.
Table 1 Osthole and Ningnanmycin compound proportion scope and composite illustration
Annotate: listed ratio is all calculated by medicament active ingredient in the table.
It is standby then above-mentioned female medicine and composite dose to be diluted to the series concentration soup by table 2.
Each medicine series diluted concentration gradient of table 2
Bacterium liquid is prepared:
The white powder germ that to gather from the field scrapes, and is made into conidial suspension with sterile water, and concentration is in 10 * 10 times each visual field 10-20 conidium of microscopically.
Condition determination and standard:
The soup of even spraying different disposal concentration on the fresh strawberry plant leaf, behind the 24h on every blade drop 3 points, every some 5ul spore liquid.Record morbidity progression calculates disease and refers to and preventive effect behind the 4d.
Disease refers to investigate grade scale:
0 grade: no scab;
1 grade: lesion area accounts for below 5% of whole leaf area;
3 grades: lesion area accounts for 6%～10% of whole leaf area;
5 grades: lesion area accounts for 11%～25% of whole leaf area;
7 grades: lesion area accounts for 26%～50% of whole leaf area;
9 grades: lesion area accounts for more than 50% of whole leaf area.
Computational methods:
Calculate preventive effect according to formula, utilize the percentage and the probit value table of comparisons to convert preventive effect to probit value, simultaneously dose concentration is converted into logarithm value, obtains independent formula Y=a+bX, calculate concentration EC50 in correlation coefficient r and the inhibition with dosage logarithm value (X) and probit value (y).
The synergistic effect of calculating mixed agent according to the method for Wadley, i.e. SR＜0.5, then two kinds of medicaments antagonism that has been mixed; 0.5≤SR≤1.5, then two kinds of medicaments summation action that has been mixed; SR＞1.5, then two kinds of medicaments synergistic effect that has been mixed.
Result and analysis:
Osthole and S-Ethyl ethylthio sulfonate and composite dose of virulence regression equation y=a+bX thereof to the powdery mildew of strawberry bacterium, correlation coefficient r, and the coefficient of synergism SR of concentration EC50 (ug/ml) and mixture sees Table 3 in effectively suppressing.
Table 3 Osthole and Ningnanmycin and compound sterilizing combination thereof are to the indoor prescription The selection result of powdery mildew of strawberry
As can be seen from Table 3, Osthole wants high to the specific activity Ningnanmycin of powdery mildew of strawberry bacterium under condition of living body; Its composite back coefficient of synergism has synergistic effect preferably all greater than 1.5.In addition, there is not mutual pesticide resistance problem in these two kinds of medicament mechanism of action differences, can be used for alleviating the resistance problem of Osthole so this two classes medicament is mixed, and prolong the service life of medicament.
For further verifying the practical effect of complex preparation control crop powdery mildew, on strawberry, grape, tomato, prevent and treat the demonstration test of powdery mildew, and rice sheath blight disease has been carried out field trial.
Two, Osthole and Ningnanmycin and compound sterilizing combination preparation field control effectiveness test thereof
" pesticide field efficacy medicine test criterion " by the chief editor of The Institute for the Control of Agrochemicals of the Ministry of Agriculture,PRC designs the field control effectiveness test of complex preparation to several crop powdery mildews and rice sheath blight disease.
(1) each medicament is to the crop powdery mildew field control effect:
Test medicine:
Handle 1:1% Osthole EW800 doubly, Jiangsu Province Su Kenongization Co., Ltd provides.
Handle 2:2% Ningnanmycin AS500 doubly, Seoul Heilongjiang Qianger biochemical technology development Co., Ltd produces.
Doubly (active ingredient: 2% Osthole+0.2% Ningnanmycin), the Zhenjiang institute of agricultural sciences develops to handle 3:2.2% Osthole Ningnanmycin EW800.
Doubly (active ingredient: 2% Osthole+0.4% Ningnanmycin), the Zhenjiang institute of agricultural sciences develops to handle 3:2.4% Osthole Ningnanmycin EW800.
Doubly (active ingredient: 1% Osthole+1% Ningnanmycin), the Zhenjiang institute of agricultural sciences develops to handle 4:2% Osthole Ningnanmycin EW800.
Doubly (active ingredient: 0.2% Osthole+2% Ningnanmycin), the Zhenjiang institute of agricultural sciences develops to handle 5:2.2% Osthole Ningnanmycin EW800.
Wherein, EW represents that aqueous emulsion, AS represent that aqua, EC represent that missible oil, WP represent wetting powder, microemulsion ME.
Experimental result:
See Table 4, Osthole and Ningnanmycin complex preparation all have the better prevention effect to strawberry, grape, tomato powdery mildew, are higher than or are equivalent to the preventive effect of each single preparation; Handle 3:2.2% Osthole Ningnanmycin EW800 doubly; Processing 4:2.4% Osthole Ningnanmycin EW800 doubly handles 5:2% Osthole Ningnanmycin EW800 doubly, shines the pharmacy control efficacy significant difference with comparison; Handle 6:2.2% Osthole Ningnanmycin EW800 doubly, suitable with the preventive effect of contrast medicament, because of the preparation of science after composite can effectively be prevented and treated the harm of white powder germ to each crop.
Observe according to system behind the medicine, do not see during the reagent that obvious poisoning takes place, each test medicine has no adverse effects to plant growth.
Table 4 Osthole and Ningnanmycin and complex preparation thereof are prevented and treated various crop powdery mildew field efficacy experimental results
Table 4 preventive effect is 3 mean values that repeat
(2) each medicament is to the rice sheath blight disease field control effect:
Test medicine:
Handle 1:1% Osthole EW800 doubly, Jiangsu Province Su Kenongization Co., Ltd provides.
Handle 2:2% Ningnanmycin AS500 doubly, Seoul Heilongjiang Qianger biochemical technology development Co., Ltd produces.
Doubly (active ingredient: 2% Osthole+0.2% Ningnanmycin), the Zhenjiang institute of agricultural sciences develops to handle 3:2.2% Osthole Ningnanmycin EW800.
Doubly (active ingredient: 2% Osthole+0.4% Ningnanmycin), the Zhenjiang institute of agricultural sciences develops to handle 4:2.4% Osthole Ningnanmycin EW800.
Doubly (active ingredient: 1% Osthole+1% Ningnanmycin), the Zhenjiang institute of agricultural sciences develops to handle 5:2% Osthole Ningnanmycin EW800.
Doubly (active ingredient: 0.2% Osthole+2% Ningnanmycin), the Zhenjiang institute of agricultural sciences develops to handle 6:2.2% Osthole Ningnanmycin EW800.
Handle 7: the contrast medicament, 1000 times of 20% jinggangmeisu water solube powders, Jiangsu greenery chemical industry Co., Ltd produces.
Wherein, EW represents that aqueous emulsion, AS represent that aqua, EC represent missible oil.
Result of the test:
See Table 5, Osthole and Ningnanmycin complex preparation have the better prevention effect to rice sheath blight disease, be higher than its each single agent and the preventive effect of handling the 7:20% jinggangmeisu, 2% Ningnanmycin AS500 is the preventive effect of single preparation doubly, handles 3:% Osthole Ningnanmycin EW800 doubly; Handle 4:2.4% Osthole Ningnanmycin EW800 doubly, it is suitable to handle EW800 times of three's preventive effect of 5:%% Osthole Ningnanmycin, all reach more than 70%, EW800 times of preventive effect of 6:2%% Osthole Ningnanmycin is high more than 7% than handling, therefore the remarkable preventive effect of the two compound synergic improves, and can effectively prevent and treat rice sheath blight disease.
Observe according to system behind the medicine, do not see during the reagent that obvious poisoning takes place, each test medicine has no adverse effects to plant growth.
Table 5 Osthole and Ningnanmycin and complex preparation thereof are prevented and treated rice sheath blight disease field efficacy experimental result
Table 5 preventive effect is 3 mean values that repeat
Specific embodiments of the invention are as follows:
Embodiment 1:2.2% Osthole Ningnanmycin EW800 is (active ingredient: 2% Osthole+0.2% Ningnanmycin) doubly, control strawberry, tomato, squash marble dust and rice sheath blight disease medication at the initial stage of a disease, every 667m2 dosage 60～80ml, later stage, every 667m2 dosage reached 100～120ml along with blade increases.
Embodiment 2:2.2% Osthole Ningnanmycin EW800 is (active ingredient: 2% Osthole+0.4% Ningnanmycin) control strawberry, tomato, squash marble dust and rice sheath blight disease medication at the initial stage of a disease doubly, every 667m2 dosage 60～-80ml, later stage, every 667m2 dosage reached 100～120ml along with blade increases.
Embodiment 3:2% Osthole Ningnanmycin EW800 times (active ingredient: 1% Osthole+1% Ningnanmycin) prevent and treat strawberry, tomato, squash marble dust and rice sheath blight disease at the initial stage of a disease, every 667m2 dosage 60～80ml, medication was 1 time in per 5～7 days.Later stage, every 667m2 dosage reached 100～120ml along with blade increases.
Embodiment 4:2.2% Osthole Ningnanmycin EW800 is (active ingredient: 0.2% Osthole+2% Ningnanmycin) doubly.Control strawberry, tomato, squash marble dust and rice sheath blight disease begin spraying at the initial stage of a disease, every 667m2 dosage 60～80ml, spray in per 5～7 days 1 time.Later stage, every 667m2 dosage reached 100～120ml along with blade increases.
Claims (2)
1. Osthole and Ningnanmycin composite bactericide, it is characterized in that: its active ingredient is Osthole and Ningnanmycin, the weight ratio of Osthole and Ningnanmycin is 1:10～10:1 in its composition.
2. Osthole according to claim 1 and Ningnanmycin composite bactericide is characterized in that: this bactericide is a kind of in the microemulsion that formed by active ingredient and auxiliary agent and carrier combinations, aqueous emulsion, water dispersible granules, the wetting powder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2011101398262A CN102293205A (en) | 2011-05-27 | 2011-05-27 | Osthole and ningnanmycin compound bactericide |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011101398262A CN102293205A (en) | 2011-05-27 | 2011-05-27 | Osthole and ningnanmycin compound bactericide |
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| CN2011101398262A Pending CN102293205A (en) | 2011-05-27 | 2011-05-27 | Osthole and ningnanmycin compound bactericide |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103300023A (en) * | 2013-06-19 | 2013-09-18 | 北京燕化永乐农药有限公司 | Pesticide composition |
| CN103329946A (en) * | 2013-06-21 | 2013-10-02 | 苏州市阳澄湖现代农业发展有限公司 | Oil suspension pesticide composition for preventing and treating rice sheath blight disease and preparation method thereof |
| CN103329947A (en) * | 2013-06-21 | 2013-10-02 | 苏州市阳澄湖现代农业发展有限公司 | Biological pesticide composition for preventing and treating rice sheath blight disease and preparation method thereof |
| CN103340216A (en) * | 2013-06-21 | 2013-10-09 | 苏州市阳澄湖现代农业发展有限公司 | Pollution-free pesticide microcapsule suspending preparation and preparation method thereof |
| CN104351187A (en) * | 2014-11-11 | 2015-02-18 | 济南新起点医药科技有限公司 | Compounded fungicide with osthole and pyrimethanil as effective ingredients |
| US9192166B2 (en) | 2013-02-11 | 2015-11-24 | Bayer Cropscience Lp | Compositions comprising a streptomyces-based biological control agent and an insecticide |
| CN114982770A (en) * | 2022-06-16 | 2022-09-02 | 江西省农业科学院植物保护研究所 | Composition for preventing and treating sesame wilt and application thereof |
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| CN1093869A (en) * | 1993-04-23 | 1994-10-26 | 中国科学院成都生物研究所 | A kind of new antibiotic pesticides-Ningnan Meisu |
| CN101347116A (en) * | 2007-07-19 | 2009-01-21 | 武汉天惠生物工程有限公司 | Complex pesticide of osthole and agricultural antibiotic and uses thereof |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9192166B2 (en) | 2013-02-11 | 2015-11-24 | Bayer Cropscience Lp | Compositions comprising a streptomyces-based biological control agent and an insecticide |
| CN103300023A (en) * | 2013-06-19 | 2013-09-18 | 北京燕化永乐农药有限公司 | Pesticide composition |
| CN103329946A (en) * | 2013-06-21 | 2013-10-02 | 苏州市阳澄湖现代农业发展有限公司 | Oil suspension pesticide composition for preventing and treating rice sheath blight disease and preparation method thereof |
| CN103329947A (en) * | 2013-06-21 | 2013-10-02 | 苏州市阳澄湖现代农业发展有限公司 | Biological pesticide composition for preventing and treating rice sheath blight disease and preparation method thereof |
| CN103340216A (en) * | 2013-06-21 | 2013-10-09 | 苏州市阳澄湖现代农业发展有限公司 | Pollution-free pesticide microcapsule suspending preparation and preparation method thereof |
| CN103329946B (en) * | 2013-06-21 | 2015-06-17 | 苏州市阳澄湖现代农业发展有限公司 | Oil suspension pesticide composition for preventing and treating rice sheath blight disease and preparation method thereof |
| CN103340216B (en) * | 2013-06-21 | 2015-08-05 | 苏州市阳澄湖现代农业发展有限公司 | A kind of public nuisance-free agricultural chemicals microcapsule suspending agent and preparation method thereof |
| CN103329947B (en) * | 2013-06-21 | 2015-08-19 | 苏州市阳澄湖现代农业发展有限公司 | Biological pesticide composite of a kind of water prevention rice sheath blight and preparation method thereof |
| CN104351187A (en) * | 2014-11-11 | 2015-02-18 | 济南新起点医药科技有限公司 | Compounded fungicide with osthole and pyrimethanil as effective ingredients |
| CN104351187B (en) * | 2014-11-11 | 2016-08-24 | 青岛青知企业管理咨询有限公司 | Active ingredient is the compound disinfectant of Osthole and pyrimethanil |
| CN114982770A (en) * | 2022-06-16 | 2022-09-02 | 江西省农业科学院植物保护研究所 | Composition for preventing and treating sesame wilt and application thereof |
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Application publication date: 20111228 |