CN106234467A - A kind of antibacterial and preparation method thereof - Google Patents
A kind of antibacterial and preparation method thereof Download PDFInfo
- Publication number
- CN106234467A CN106234467A CN201610650588.4A CN201610650588A CN106234467A CN 106234467 A CN106234467 A CN 106234467A CN 201610650588 A CN201610650588 A CN 201610650588A CN 106234467 A CN106234467 A CN 106234467A
- Authority
- CN
- China
- Prior art keywords
- antibacterial
- parts
- oil
- herba
- volatile oil
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/40—Liliopsida [monocotyledons]
- A01N65/42—Aloeaceae [Aloe family] or Liliaceae [Lily family], e.g. aloe, veratrum, onion, garlic or chives
Abstract
The present invention relates to technical field of pesticide, disclose a kind of antibacterial and preparation method thereof.This antibacterial is made up of the raw material of following parts by weight: solidago canadesis volatile oil 5 10 parts, jingganmycin 6 11 parts, 35 parts of Herba Alii fistulosi, 15 30 parts of Radix Glycyrrhizae, Herba Portulacae 3 14 parts, 2 10 parts of Pericarpium Zanthoxyli, Bitertanol 27 parts, solvent 25 41 parts, cosolvent 11 16 parts and antifreezing agent 2 12 parts.The antibacterial of the present invention has that low toxicity, environmental protection, fungicidal spectrum be wide, lasting period length, effectively slow down drug-fast advantage, is to utilize in cultural control crop pest.
Description
Technical field
The present invention relates to technical field of pesticide, particularly relate to a kind of antibacterial and preparation method thereof.
Background technology
In recent years, due to the people's unreasonable use to chemical pesticide and the inherent defect such as fungicidal spectrum of medicament itself thereof
Single, it is used for multiple times and is easily generated Drug resistance, medicine component toxicity is the strongest, and it is a series of that these make pesticide in use create
Pollution problem, such as environmental pollution, person poultry poisoning, killing natural enemy, destroy ecological balance, " 3R " (Residue, Resistance,
The serious problems such as Resurgence), these harmful effects have caused the common concern of people.In recent years on pesticide market, domestic
Antibacterial is sold and is glided always, mainly can not show a candle to the preventive effect of external antibacterial because of its preventive effect controlling disease.And develop in China
A kind of novel pesticide composition, Meteorological is high, and technology is immature.Though external novel pesticide composition drug effect is good, but price is high.Therefore,
It is to develop pesticide new product at present and prevent and treat the one of resistance disease that the pesticide active ingredient utilizing the different mechanism of action carries out compounding
Plant effective quick way.Different pesticide active ingredients carry out compounding after, three kinds of type of action can be shown: summation action, increasing
Effect effect and antagonism.Generally, only by substantial amounts of test just can learn different pesticide active ingredient compounding after effect
Type.
China's plant resources is the abundantest, has the higher plant species of made to reach more than 30,000 kinds, is subordinate to 470 sections,
Wherein 1300 various plants have Fungicidal active substance, are distributed in 140 sections." Zhou Li " has cloud, and " the Jian Shi palm, except moth-eaten thing, is attacked to attack to entangle
It, smoke it with Folium illicii Lanceolati "；Compendium of Material Medica mentions the Radix Euphorbiae Fischerianae (Radix Euphorbiae Ebracteolatae), the Radix Stemonae, derris root etc.；" China's soil pesticide will " recites and is distributed in 86
220 kinds of botanical pesticides in individual section, then list poisonous plants more than 1300 in " China poisonous plants " book and plant, many of which
The existing history utilized as botanical pesticide of kind.Utilize natural plants as Pesticide use more preferably Environmental Safety.
Although achieving some achievements in the current research to botanical fungicide composition, but great majority all existing simply
Indivedual a few class diseases are carried out effect experiment, and range is limited, and has only focused on preventive effect, whether produces its Drug resistance
Raw, use the problems such as harm that biological invasion itself had how alleviation not make and further study.Such as China
The patent CN 102763689 A mono-kind compound disinfectant containing solidago canadesis extract, this invention remains with existing
Chemical pesticide be used in mixed way, although serving certain potentiation, but simply powdery mildew is had potentiation, as other
Common Diseases such as downy mildew, anthrax, gray mold etc. do not carry out effect experiment, and fungicidal spectrum is limited in scope, and for
The solidago canadesis itself belonging to biological invasion uses and the side effect of environment may the most not proposed generation solution.
For disease drug resistance research also without reference to.
Summary of the invention
It is an object of the invention to: for the problem of above-mentioned existence, it is provided that a kind of preventive effect is good, and fungicidal spectrum is wide, and consumption is few, energy
Effectively slow down the generation of disease drug resistance, and the antibacterial of pollution reducing environment and preparation method thereof.
In order to realize foregoing invention purpose, the technical solution used in the present invention is as follows:
A kind of antibacterial, is made up of the raw material of following parts by weight: solidago canadesis volatile oil 5-10 part, jingganmycin 6-
11 parts, Herba Alii fistulosi 3-5 part, Radix Glycyrrhizae 15-30 part, Herba Portulacae 5-12 part, Pericarpium Zanthoxyli 4-9 part, Bitertanol 2-7 part, solvent 25-41
Part, cosolvent 11-16 part and antifreezing agent 2-12 part.
Further, described antibacterial, it is made up of the raw material of following parts by weight: solidago canadesis volatile oil 6-
8 parts, jingganmycin 6-11 part, Herba Alii fistulosi 4-4.5 part, Radix Glycyrrhizae 18-25 part, Herba Portulacae 5-12 part, Pericarpium Zanthoxyli 4-9 part, Bitertanol
3-6 part, solvent 31-37 part, cosolvent 11-14 part and antifreezing agent 3-6 part.
Further, described antibacterial, it is made up of the raw material of following parts by weight: solidago canadesis volatile oil 7
Part, jingganmycin 8 parts, 4.5 parts of Herba Alii fistulosi, 20 parts of Radix Glycyrrhizae, Herba Portulacae 9 parts, 8.5 parts of Pericarpium Zanthoxyli, Bitertanol 5 parts, solvent 35 parts,
Cosolvent 13 parts and antifreezing agent 4 parts.
Further, described solvent be Oleum Arachidis hypogaeae semen, Oleum Brassicae campestris, soybean oil, Oleum sesami, Petiolus Trachycarpi oil, olive oil, Semen Maydis oil and
One or more in mineral oil, preferably soybean oil；Cosolvent is in acetone, chloroform, benzene, dimethylbenzene, methanol or ethanol
Plant or several, preferred alcohol；Antifreezing agent is the one in ethylene glycol, propylene glycol, glycerol, Polyethylene Glycol or isopropanol or several
Kind, preferably isopropanol.
Present invention also offers the preparation method of antibacterial described above, comprise the steps of:
(1) from plant intermediate acquisition Herba Alii fistulosi, Radix Glycyrrhizae, Herba Portulacae, Pericarpium Zanthoxyli, the coarse powder of 3-6mm then it is crushed to plant pulverizer
Mince, coarse powder is minced by soak with ethanol 2-3 that concentration is 75-85% month, extract supernatant standby, i.e. the plant of antibacterial
Source extracting solution；
(2) distilling, separate: put into by solidago canadesis in boiler, add water in pot dipped solidago canadesis, the bottom of a pan
Heating, steam obtains the liquid mixture of water and quintessence oil in pot top gooseneck imports condenser, obtains after oil-water separation
Solidago canadesis volatile oil；
(3) mix, grind: in the antibacterial plant source extracting solution that step (1) obtains, add the Canada one that step (2) obtains
Branch Hemerocallis citrina Baroni volatile oil and Bitertanol, to be mixed uniformly after proportionally add solvent and cosolvent and dissolve, mixing
Uniformly, then grind 20-35 minute with colloid mill, continue after adding antifreezing agent to grind 20-35 minute on colloid mill, killed
Microbial inoculum colloid；
(4) stirring: antibacterial colloid ground for step (3) is moved in emulsifying kettle, adds distilled water to antibacterial colloid full dose
2/3, under the rotating speed of 500-12000 rev/min stir 20-30 minute, i.e. obtain the aqueous emulsion of antibacterial.
Further, in step (4), described stirring means is as follows: by antibacterial colloid ground in step (3)
Move in emulsifying kettle, add distilled water to the 2/3 of antibacterial colloid full dose, under the rotating speed of 6000 revs/min, stir 25 minutes, i.e.
Obtain the aqueous emulsion of antibacterial.
In described raw material, each component brief introduction is as follows:
Solidago canadesis (Solidago canadensis) also known as Solidago Canadensis, belong to Compositae one
Hemerocallis citrina Baroni belongs to branch, although it belongs to biological invasion, is agriculturally being referred to as evil grass, but years of researches show that it has widely
Prospect in medicine, is allowed as medical material in many countries.Solidago canadesis have antiinflammatory, spasmolytic, diuresis, treatment wound and
Strengthen the effect of renal function.Solidago canadesis volatile oil is employing water vapour from the leaf of solidago canadesis, flower, seed
The way of distillation extracts the volatile oil obtained, containing the elemene chemical combination that relative amount is higher in solidago canadesis leaf volatile oil
Thing, can be sprayed at the organs such as the stem of plant, leaf.Germacrene-D in solidago canadesis volatile oil, D-limonene note has anti-
Bacterium activity, has a broad antifungal spectrum, can substantially suppress the breeding of the pathogenic bacterias such as staphylococcus aureus, escherichia coli and bacillus subtilis,
Solidago canadesis volatile oil and composite jinggangmycin can strengthen the antibacterial activity of main component germacrene-D in volatile oil,
Overcome the shortcoming that germacrene-D antibacterial activity is the most relatively low.Solidago canadesis is added in antibacterial of the present invention
Volatile oil is turned waste into wealth, and is reused by invasive species influential on ecological environment, reaches to improve bactericidal effect, expands
The effect of antimicrobial spectrum, provides a new thinking for solidago canadesis integrated control simultaneously.
Jingganmycin microorganism belonging to genus source, antibiotics, low toxicity antibacterial.Main dosage form: 3%, 5%, 10% water preparation, 2%, 3%,
4%, 5%, 10%, 12%, 15%, 17%, 20%, 40%, 60% solvable powder, 0.33% powder.There are treatment and protective effect, are mainly used in
Prevent and treat all kinds of banded sclerotial blight it can also be used to prevent and treat melon damping-off, root rot etc., be the high-effective broad-spectrum fungicide of a kind of low resistance.
Bitertanol is ergosterol synthesising preparation, does not suppress spore germination, but can suppress mycelial growth, belongs to not with jingganmycin
With the synthesis pathogenic bacteria inhibitor of type, both use with and play potentiation and expand effect jingganmycin and the Bitertanol of fungicidal spectrum
Mixture can increase the preventive effect to rice sheath blight disease after using so that it is can have wider in south rice area and prevent curing the disease
Evil space.
Herba Alii fistulosi (Allium fistulosum L.var.giganteum MakionIn)
Antipathogenic composition come from nature, being compatible with the environment property is high, noresidue, safer to people and animals, pathogen and evil bacterium natural enemy,
It is not likely to produce Drug resistance.China's Herba Alii fistulosi aboundresources and low price, have important value in agricultural production.Press down as Herba Alii fistulosi
The main active liposoluble constituent of bacterium is respectively provided with inhibitory action to antibacterial and fungus, wherein has Staphylococcus albus very
Strong inhibitory action, after constant temperature culture 24h, total plate count have dropped 80.65%, also has escherichia coli and bacillus subtilis
Certain inhibitory action, after constant temperature culture 24h, total plate count have dropped 71.38% pair of rhodotorula glutinis aspergillus niger and also has suppression
Ability.After constant temperature culture 24h, total plate count have dropped 63.51%.The liposoluble constituent of Herba Alii fistulosi mixes with Herba Portulacae can increase horse
The hydrophilic of bitterroot, increases the major antibacterial composition flavone compound in Herba Portulacae further in antibacterial of the present invention
Dissolubility, gives full play to its effectiveness.
Radix Glycyrrhizae (glycyrrhiza uralensis Fisch) it is that a kind of perennial pulse family is planted
Thing, for root and the rhizome of pulse family Glycyrrhiza minister in ancient times's various plants.Glycyrrhiza uralensis Fisch., Glycyrrhiza inflata Bat. are favored with.And Glycyrrhiza glabra L., it is China
Distinctive Chinese medicine, in China's treatment disease, oneself has history for many years for it, by the king that successive dynasties well-known doctor's honor is many medicines.China is among the people
Have very early and utilize Radix Platycodonis, Radix Glycyrrhizae compatibility to treat toothache, stomatitis, the history of periodontitis, the glycyrrhizic acid in Radix Glycyrrhizae and Radix Glycyrrhizae time
Acid has bacteriostasis, and wherein the optical isomer of enoxolone has good inhibitory action to gram positive bacteria.
Herba Portulacae (Portulaca oleracea L.) aboundresources, it is that medical, edible is all
Good wild plant, it had both contained multiple nutritional components, had contained again higher flavone compound, and flavone compound is
The big effective constituents of the one of medicinal plants, has multiple biological activity, and one of its Main Function is antibacterial or sterilization, during low concentration
Can be antibacterial, can sterilize during high concentration.Herba Portulacae mixes with the liposoluble constituent of Herba Alii fistulosi can increase the hydrophilic of Herba Portulacae, further
Ground increases the dissolubility in antibacterial of the present invention of the major antibacterial composition flavone compound in Herba Portulacae, gives full play to its effect
With.
Pericarpium Zanthoxyli (Zanthoxylum bungeanum Maxim.), another name:, big green pepper,
Pericarpium Zanthoxyli, another name for Sichuan Province green pepper, Fructus Zanthoxyli or Pericarpium Zanthoxyli.For Rutaceae, Zanthoxylum machaka or dungarunga, can isolated planting can make again to protect thorn hedge.
Pericarpium Zanthoxyli is warm in nature, acrid in the mouth, can play the effect of mediation in antibacterial, neutralizes medicine Acidity of Aikalinity, pesticide control meta-acid or meta-alkali
And plant leaf is produced and destroys.
Bitertanol, with Bitertanol for parent pesticide, has the life of polymerizable double bond by chemical reactive synthesis
Thing activated monomer, then through and acrylic acid copolymer, prepare pendant side chain with the polymer chemical of Bitertanol active part
Slow-release bactericide.This high molecular slow-release antibacterial is in field test, through processes such as hydrolysis and/or microbial degradations, and can be to have
Effect dosage sustained release goes out to have the component of bactericidal activity.Bitertanol is ergosterol synthesising preparation, does not suppress spore to sprout
Sending out, but can suppress mycelial growth, belong to different types of synthesis pathogenic bacteria inhibitor with jingganmycin, both are mixed each by complementation
From physicochemical property be not enough to play potentiation and expand the effect of fungicidal spectrum, jingganmycin and Bitertanol mixture can after using
To increase preventive effect to rice sheath blight disease so that it is can there be in south rice area controlling disease space wider.
In sum, owing to have employed technique scheme, the invention has the beneficial effects as follows:
(1) scientific compatibility: a kind of antibacterial of the present invention make use of the principle of scientific compatibility, by solidago canadesis volatile oil
Mix with jingganmycin, utilize jingganmycin to improve solidago canadesis volatile oil main antimicrobial component germacrene-D
Antibacterial activity, overcomes the shortcoming that germacrene-D antibacterial activity is the most relatively low, adds prevention effect, compare Canada
Herba Solidaginis volatile oil single dose uses preventive effect averagely to improve 27.47%, serves the effect of potentiation.Bitertanol is Ergota
Sterol synthesising preparation, does not suppress spore germination, but can suppress mycelial growth, belongs to different types of synthesis pathogenic bacteria with jingganmycin
Inhibitor, compensate for the deficiency of respective physicochemical property after both are mixed, play potentiation and expand the effect of fungicidal spectrum, Jing Gang simultaneously
Mycin and Bitertanol mixture can increase the preventive effect to rice sheath blight disease after using, and improve jingganmycin to rice banded sclerotial blight
The problem that sick preventive effect is more weak so that it is can have in south rice area controlling disease space wider.Herba Portulacae and Herba Alii fistulosi
Liposoluble constituent mixing can increase the hydrophilic of Herba Portulacae, increase the major antibacterial composition flavonoid in Herba Portulacae further
Compound dissolubility in antibacterial of the present invention, gives full play to its effectiveness.Pericarpium Zanthoxyli can play the effect of mediation in antibacterial,
Neutralize medicine Acidity of Aikalinity, pesticide control meta-acid or meta-alkali and to plant leaf produce destroy.Antibacterial of the present invention is by by each
Beneficiating ingredient scientific compatibility solves deficiency when respective component single dose uses, and expands fungicidal spectrum, serves Synergistic and makees
With, farmland Common Diseases part preventive effects such as rice sheath blight disease, false smut, cucumber downy mildew all can be reached 83.24%.
(2) low toxicity, environmental protection.Soybean oil in the antibacterial of the present invention is solvent, and antifreezing agent is isopropanol, and cosolvent is second
Alcohol, and primary raw material source is the natural plant in the nature such as Herba Portulacae, Pericarpium Zanthoxyli, becomes with chemical pesticide conventional on the market
Dividing difference, unartificial chemosynthesis, therefore toxicity is low, and antibacterial is degradable after being spray applied, and does not interferes with ecological environment.Pericarpium Zanthoxyli
In antibacterial of the present invention as regulator in and medicine Acidity of Aikalinity, pesticide control meta-acid or meta-alkali and to plant leaf produce
Destroy so that medicament uses safer, environmental protection.Solidago canadesis volatile oil and composite jinggangmycin can strengthen volatile oil
The antibacterial activity of middle main component germacrene-D, overcomes the shortcoming that germacrene-D antibacterial activity is the most relatively low, simultaneously
Jingganmycin adds solidago canadesis volatile oil dissolubility in the middle of antibacterial of the present invention, prevents Canada's solidago plant
Toxic component in spending, to external diffusion, utilizes the abundant controlling disease of its effective ingredient.Therefore the effect of low toxic and environment-friendly has been reached.
(3) comprehensively utilizing, turn waste into wealth: solidago canadesis belongs to Invasive Alien Species, its amount reproduction can destroy
The stability of Agro-ecological System, but the germacrene-D in solidago canadesis volatile oil component passes through and jingganmycin
Mixing after can be remarkably reinforced its bactericidal activity, preventive effect is compared solidago canadesis volatile oil single dose and to be improved
27.47%, compare fungicide carbendazim conventional on the market and to improve 36.7%, overcome solidago canadesis volatile oil at room temperature
The shortcoming that lower bactericidal activity is relatively low.The use of jingganmycin simultaneously adds solidago canadesis volatile oil and sterilizes in the present invention
Dissolubility in the middle of agent, prevent the toxic component in solidago canadesis to external diffusion, therefore to solidago canadesis this
Class biological invasion, is made full use of some harm prevented and treated agriculturally, not only increases the utilization ratio of biological invasion, become
Waste be changed into values, and the preventing and treating for biological invasion in the future provides a new thinking.
(4) fungicidal spectrum is wide, the lasting period is long.In the antibacterial of the present invention containing jingganmycin and Bitertanol, Jing Gang
Mycin belongs to antibiotics antibacterial, and Bitertanol belongs to ergosterol synthetic inhibitor, and jingganmycin is for wheat crops
Disease all has good preventive effect, but limited for Oryza sativa L. class diseases control, and repeatedly continuous administration easily develops immunity to drugs, impact
Its promoting the use of in southern area, but Bitertanol ergosterol synthesising preparation, do not suppress spore germination, but can suppress
Mycelial growth, belongs to different synthetic inhibitors from jingganmycin, has good preventive effect to rice disease, and both are mixed can increase
Preventive effect to rice disease, all reaches 83.16% to the preventive effect of common rice disease such as rice sheath blight disease and false smut.Dens Equi
Herba Amaranthi tricoloris mixes with the liposoluble constituent of Herba Alii fistulosi can increase the hydrophilic of Herba Portulacae, and the major antibacterial increased further in Herba Portulacae becomes
Divide flavone compound dissolubility in antibacterial of the present invention, and then add the medicament tack to plant, prevent rainwater
Wash away, extend the preventive effect phase, in effect experiment, a kind of antibacterial of the present invention to experiment disease average imitation reach
85.83%, and after six months the same area evil bacterium preventive effect still can be maintained about 81.82%, and key component individually makes
15.89% is averagely have dropped with chemical pesticide preventive effect after six months conventional on the market.
Detailed description of the invention
One, a kind of antibacterial and preparation method thereof
Embodiment 1
A kind of antibacterial, is made up of the raw material of following parts by weight: solidago canadesis volatile oil 5 parts, jingganmycin 11 parts,
3 parts of Herba Alii fistulosi, 30 parts of Radix Glycyrrhizae, Herba Portulacae 3 parts, 10 parts of Pericarpium Zanthoxyli, Bitertanol 2 parts, solvent 41 parts, cosolvent 11 parts and antifreezing agent
12 parts.
Present invention also offers the preparation method of antibacterial described above, comprise the steps of:
(1) from plant intermediate acquisition Herba Alii fistulosi, Radix Glycyrrhizae, Herba Portulacae, Pericarpium Zanthoxyli, the coarse pulverization of 4mm then it is crushed to plant pulverizer
Thing, minces coarse powder by the soak with ethanol that concentration is 85% 3 months, extracts supernatant standby, i.e. the plant source of antibacterial extracts
Liquid；
(2) distilling, separate: put into by solidago canadesis in boiler, add water in pot dipped solidago canadesis, the bottom of a pan
Heating, steam obtains the liquid mixture of water and quintessence oil in pot top gooseneck imports condenser, obtains after oil-water separation
Solidago canadesis volatile oil；
(3) mix, grind: in the antibacterial plant source extracting solution that step (1) obtains, add the Canada one that step (2) obtains
Branch Hemerocallis citrina Baroni volatile oil and Bitertanol, to be mixed uniformly after proportionally add solvent and cosolvent and dissolve, mixing
Uniformly, then grind 20 minutes with colloid mill, continue after adding antifreezing agent to grind 35 minutes on colloid mill, obtain antibacterial glue
Body；
(4) stirring: antibacterial colloid ground for step (3) is moved in emulsifying kettle, adds distilled water to antibacterial colloid full dose
2/3, under the rotating speed of 12000 revs/min stir 25 minutes, i.e. obtain the aqueous emulsion of antibacterial.
Embodiment 2
A kind of antibacterial, is made up of the raw material of following parts by weight: solidago canadesis volatile oil 10 parts, jingganmycin 6 parts,
5 parts of Herba Alii fistulosi, 15 parts of Radix Glycyrrhizae, Herba Portulacae 14 parts, 2 parts of Pericarpium Zanthoxyli, Bitertanol 7 parts, solvent 25 parts, cosolvent 16 parts and antifreezing agent
2 parts.
Its preparation method is same as in Example 1.
Embodiment 3
A kind of antibacterial, is made up of the raw material of following parts by weight: solidago canadesis volatile oil 6 parts, jingganmycin 11 parts,
3 parts of Herba Alii fistulosi, 30 parts of Radix Glycyrrhizae, Herba Portulacae 3 parts, 10 parts of Pericarpium Zanthoxyli, Bitertanol 2 parts, solvent 41 parts, cosolvent 11 parts and antifreezing agent
12 parts.
Present invention also offers the preparation method of antibacterial described above, comprise the steps of:
(1) from plant intermediate acquisition Herba Alii fistulosi, Radix Glycyrrhizae, Herba Portulacae, Pericarpium Zanthoxyli, the coarse pulverization of 6mm then it is crushed to plant pulverizer
Thing, minces coarse powder by the soak with ethanol that concentration is 80% 3 months, extracts supernatant standby, i.e. the plant source of antibacterial extracts
Liquid；
(2) distilling, separate: put into by solidago canadesis in boiler, add water in pot dipped solidago canadesis, the bottom of a pan
Heating, steam obtains the liquid mixture of water and quintessence oil in pot top gooseneck imports condenser, obtains after oil-water separation
Solidago canadesis volatile oil；
(3) mix, grind: in the antibacterial plant source extracting solution that step (1) obtains, add the Canada one that step (2) obtains
Branch Hemerocallis citrina Baroni volatile oil and Bitertanol, to be mixed uniformly after proportionally add solvent and cosolvent and dissolve, mixing
Uniformly, then grind 35 minutes with colloid mill, continue after adding antifreezing agent to grind 20 minutes on colloid mill, obtain antibacterial glue
Body；
(4) stirring: antibacterial colloid ground for step (3) is moved in emulsifying kettle, adds distilled water to antibacterial colloid full dose
2/3, under the rotating speed of 500 revs/min stir 30 minutes, i.e. obtain the aqueous emulsion of antibacterial.
Embodiment 4
A kind of antibacterial, is made up of the raw material of following parts by weight: solidago canadesis volatile oil 8 parts, jingganmycin 7 parts,
4.5 parts of Herba Alii fistulosi, 18 parts of Radix Glycyrrhizae, Herba Portulacae 5 parts, 9 parts of Pericarpium Zanthoxyli, Bitertanol 3 parts, solvent 37 parts, cosolvent 11 parts and antifreeze
Agent 6 parts.
Present invention also offers the preparation method of above-mentioned antibacterial, comprise the steps of:
(1) from plant intermediate acquisition Herba Alii fistulosi, Radix Glycyrrhizae, Herba Portulacae, Pericarpium Zanthoxyli, the coarse pulverization of 3mm then it is crushed to plant pulverizer
Thing, minces coarse powder by the soak with ethanol that concentration is 85% 3 months, extracts supernatant standby, i.e. the plant source of antibacterial extracts
Liquid；
(2) distilling, separate: put into by solidago canadesis in boiler, add water in pot dipped solidago canadesis, the bottom of a pan
Heating, steam obtains the liquid mixture of water and quintessence oil in pot top gooseneck imports condenser, obtains after oil-water separation
Solidago canadesis volatile oil；
(3) mix, grind: in the antibacterial plant source extracting solution that step (1) obtains, add the Canada one that step (2) obtains
Branch Hemerocallis citrina Baroni volatile oil and Bitertanol, to be mixed uniformly after proportionally add solvent and cosolvent and dissolve, mixing
Uniformly, then grind 35 minutes with colloid mill, continue after adding antifreezing agent to grind 35 minutes on colloid mill, obtain antibacterial glue
Body；
(4) stirring: antibacterial colloid ground for step (3) is moved in emulsifying kettle, adds distilled water to antibacterial colloid full dose
2/3, under the rotating speed of 8000 revs/min stir 30 minutes, i.e. obtain the aqueous emulsion of antibacterial.
Embodiment 5
A kind of antibacterial, is made up of the raw material of following parts by weight: solidago canadesis volatile oil 7 parts, jingganmycin 8 parts,
4.5 parts of Herba Alii fistulosi, 20 parts of Radix Glycyrrhizae, Herba Portulacae 9 parts, 8.5 parts of Pericarpium Zanthoxyli, Bitertanol 5 parts, solvent 35 parts, cosolvent 13 parts and anti-
Freeze agent 4 parts.
Present invention also offers the preparation method of antibacterial described above, comprise the steps of:
(1) from plant intermediate acquisition Herba Alii fistulosi, Radix Glycyrrhizae, Herba Portulacae, Pericarpium Zanthoxyli, the coarse pulverization of 5mm then it is crushed to plant pulverizer
Thing, minces coarse powder by the soak with ethanol that concentration is 75% 3 months, extracts supernatant standby, i.e. the plant source of antibacterial extracts
Liquid；
(2) distilling, separate: put into by solidago canadesis in boiler, add water in pot dipped solidago canadesis, the bottom of a pan
Heating, steam obtains the liquid mixture of water and quintessence oil in pot top gooseneck imports condenser, obtains after oil-water separation
Solidago canadesis volatile oil；
(3) mix, grind: in the antibacterial plant source extracting solution that step (1) obtains, add the Canada one that step (2) obtains
Branch Hemerocallis citrina Baroni volatile oil and Bitertanol, to be mixed uniformly after proportionally add solvent and cosolvent and dissolve, mixing
Uniformly, then grind 35 minutes with colloid mill, continue after adding antifreezing agent to grind 20 minutes on colloid mill, obtain antibacterial glue
Body；
(4) stirring: antibacterial colloid ground for step (3) is moved in emulsifying kettle, adds distilled water to antibacterial colloid full dose
2/3, under the rotating speed of 6000 revs/min stir 25 minutes, i.e. obtain the aqueous emulsion of antibacterial.
Two, application experiment
Experiment 1: below experimental selection above-described embodiment 1 to 5 respectively with solidago canadesis volatile oil, jingganmycin, double benzene
Triadimenol and carbendazim carry out contrast test.From peasant household of Zhong He township, Yongning District, Nanning City farmland, gather classification, obtain water
The sheath and culm blight of rice, false smut, cucumber downy mildew, powdery mildew of cucumber, anthracnose of tomato.Matched group uses solidago canadesis volatilization
Oil, jingganmycin, Bitertanol and carbendazim, result of the test is shown in Table 1
Table 1 antibacterial of the present invention and the efficiency test correction data of matched group antibacterial
| Rice sheath blight disease controlling rate (%) | False smut control rate (%) | Cucumber downy mildew control rate (%) | Powdery mildew of cucumber control rate (%) | Anthracnose of tomato control rate (%) | |
| Embodiment 1 | 80.35 | 82.58 | 82.47 | 82.34 | 85.7 |
| Embodiment 2 | 82.5 | 85.76 | 82.13 | 84.28 | 84.36 |
| Embodiment 3 | 84.43 | 85.82 | 83.64 | 88.85 | 86.34 |
| Embodiment 4 | 86.42 | 88.28 | 85.17 | 87.55 | 88.29 |
| Embodiment 5 | 89.15 | 90.1 | 88.46 | 92.37 | 88.51 |
| Solidago canadesis volatile oil | 60.12 | 60.3 | 58.14 | 54.96 | 58.28 |
| Jingganmycin | 56.34 | 54 | 55.63 | 53 | 56.34 |
| Bitertanol | 61.33 | 52 | 56.24 | 59.37 | 47 |
| Carbendazim | 65.32 | 44.27 | 48.63 | 41 | 46.43 |
Higher preventive effect, average preventive effect is all had to reach five kinds for examination disease by antibacterial of the present invention seen from table 1 data
85.83%, and using the average preventive effect of solidago canadesis volatile oil is 58.36%, less than the average preventive effect of embodiment 27.47%；
The average preventive effect of jingganmycin is 55.06%, less than the average preventive effect of embodiment 30.77%；The average preventive effect of Bitertanol is
55.19%, less than the average preventive effect of embodiment 30.65%, the average preventive effect of carbendazim is 49.13%, less than the average preventive effect of embodiment
36.71%.Seeing on the whole, the average preventive effect of reference examples is 31.39%, and the average preventive effect of the present embodiment is higher than reference examples 31.39%.
Although reference examples also has preventive effect to reach 65.32%, but is only limitted to single disease, general to the preventive effect of other disease, fungicidal spectrum
Limited.And the preventive effect of antibacterial of the present invention is not limited only to test the five kinds of diseases provided, to other agricultural disease such as fresh kidney beans
Rust, capsicum epidemic disease and Fructus Benincasae anthrax etc. all have the preventive effect that above experiment effect is suitable.
Experiment 2: below experimental selection above-described embodiment 1 to 5 respectively with solidago canadesis volatile oil, jingganmycin,
Bitertanol and carbendazim carry out drug-fast contrast test.Gather from man of peasant household of Zhong He township, Yongning District, Nanning City farmland
Classification, has obtained rice sheath blight disease, cucumber downy mildew and tomato late blight.Matched group use solidago canadesis volatile oil,
Jingganmycin, Bitertanol and carbendazim, respectively the later moon used first, three months and six months again to for examination
Evil bacterium carries out effect experiment, and contrast obtains the Drug resistance power that several drugs produces.Result of the test is shown in Table 2.
Table 2 antibacterial of the present invention and matched group antibacterial are for the resistant test of rice sheath blight disease
By table 2 experimental data, the Drug resistance of rice sheath blight disease is tested, by observing it is found that embodiment is executed first
Averagely declining 3.51% by six months afterwards preventive effects, six months rear defences used by reference examples solidago canadesis volatile oil first
Effect averagely reduces 14.2%, compares embodiment and to exceed 10.68%；Reference examples jingganmycin preventive effect after using six months first
Averagely reduce 14.61%, compare embodiment and to exceed 11.09%；Reference examples Bitertanol uses six months rear defences first
Effect averagely reduces 14.62%, compares embodiment and to exceed 11.11%, reference examples carbendazim preventive effect after using six months first
Averagely reduce 20.11%, compare embodiment and to exceed 16.59%.See that the preventive effect of four reference examples is used first on the whole
Averagely have dropped 12.37% after six months, illustrate that using rear antibacterial of the present invention first does not produce substantially rice sheath blight disease
Drug resistance, and four matched groups all receive drug-fast impact for the preventive effect of rice sheath blight disease and reduce.
Table 3 antibacterial of the present invention and matched group antibacterial are for the resistant test of cucumber downy mildew
By table 3 experimental data, the Drug resistance of cucumber downy mildew is tested, by observing it is found that embodiment is executed first
Averagely declining 3.51% by six months afterwards preventive effects, six months rear defences used by reference examples solidago canadesis volatile oil first
Effect averagely reduces 12.23%, compares embodiment and to exceed 10.51%；Reference examples jingganmycin uses six months rear defences first
Effect averagely reduces 15.35%, compares embodiment and to exceed 13.63%；Reference examples Bitertanol is after using six months first
Preventive effect averagely reduces 20.1%, compares embodiment and to exceed 18.38%, reference examples carbendazim preventive effect after using six months first
Averagely reduce 18.42%, compare embodiment and to exceed 16.7%.See that the preventive effect of four reference examples is using six first on the whole
Averagely have dropped 12.24% after individual month, compare embodiment meansigma methods and exceed 10.25%.Illustrate to use rear antibacterial of the present invention first
Cucumber downy mildew is not produced obvious Drug resistance, and four matched groups all receive anti-for the preventive effect of cucumber downy mildew
The impact of the property of medicine and reduce.
, table 4 antibacterial of the present invention and matched group antibacterial are for the resistant test of tomato late blight
By table 4 experimental data, the Drug resistance of tomato late blight is tested, by observing it is found that embodiment is executed first
Averagely declining 3.29% by six months afterwards preventive effects, six months rear defences used by reference examples solidago canadesis volatile oil first
Effect averagely reduces 13.36%, compares embodiment and to exceed 10.28%；Reference examples jingganmycin uses six months rear defences first
Effect averagely reduces 15.61%, compares embodiment and to exceed 12.53%；Reference examples Bitertanol is after using six months first
Preventive effect averagely reduces 14.58%, compares embodiment and to exceed 11.5%, reference examples carbendazim preventive effect after using six months first
Averagely reduce 15.62%, compare embodiment and to exceed 12.54%.See that the preventive effect of four reference examples is used first on the whole
Averagely have dropped 11.71% after six months, illustrate that using rear antibacterial of the present invention first does not produce substantially cucumber downy mildew
Drug resistance, and four matched groups all receive drug-fast impact for the preventive effect of cucumber downy mildew and reduce.Illustrate first
Use rear antibacterial of the present invention and tomato late blight is not produced obvious Drug resistance, and four matched groups are for Phytophthora infestans
Sick preventive effect all receives drug-fast impact and reduces.Tables 2, table 3, the data of table 4 that experiment two is drawn are not limited only to water
The sheath and culm blight of rice, cucumber downy mildew and tomato late blight, for other agricultural disease, such as fresh kidney beans rust, capsicum epidemic disease and winter
Melon anthrax etc. all can effectively alleviate drug-fast generation.
The invention is not limited in aforesaid detailed description of the invention.The present invention expands to any disclose in this manual
New feature or any new combination, and the arbitrary new method that discloses or the step of process or any new combination.
Claims (7)
1. an antibacterial, it is characterised in that be made up of the raw material of following parts by weight: solidago canadesis volatile oil 5-10
Part, jingganmycin 6-11 part, Herba Alii fistulosi 3-5 part, Radix Glycyrrhizae 15-30 part, Herba Portulacae 3-14 part, Pericarpium Zanthoxyli 2-10 part, Bitertanol 2-7
Part, solvent 25-41 part, cosolvent 11-16 part and antifreezing agent 2-12 part.
A kind of antibacterial the most according to claim 1, it is characterised in that be made up of the raw material of following parts by weight: Canada
Herba Solidaginis volatile oil 6-8 part, jingganmycin 7-10 part, Herba Alii fistulosi 3.5-4.5 part, Radix Glycyrrhizae 18-25 part, Herba Portulacae 5-12 part, flower
Green pepper 4-9 part, Bitertanol 3-6 part, solvent 31-37 part, cosolvent 11-14 part and antifreezing agent 3-6 part.
A kind of antibacterial the most according to claim 1, it is characterised in that be made up of the raw material of following parts by weight: Canada
Herba Solidaginis volatile oil 7 parts, jingganmycin 8 parts, 4.2 parts of Herba Alii fistulosi, 20 parts of Radix Glycyrrhizae, Herba Portulacae 9 parts, 8.5 parts of Pericarpium Zanthoxyli, double benzotriazole
Alcohol 5 parts, solvent 35 parts, cosolvent 13 parts and antifreezing agent 4 parts.
4. according to a kind of antibacterial described in claim 1 or 2 or 3, it is characterised in that: the solvent in described raw material is Semen arachidis hypogaeae
One or more in oil, Oleum Brassicae campestris, soybean oil, Oleum sesami, Petiolus Trachycarpi oil, olive oil, Semen Maydis oil and mineral oil；Cosolvent is third
One or more in ketone, chloroform, benzene, dimethylbenzene, methanol or ethanol；Antifreezing agent is ethylene glycol, propylene glycol, glycerol, poly-second
One or more in glycol or isopropanol.
A kind of antibacterial the most according to claim 4, it is characterised in that: described solvent is soybean oil；Cosolvent is ethanol；
Antifreezing agent is isopropanol.
6. according to the preparation method of a kind of antibacterial described in claim 1 or 2 or 3, it is characterised in that comprise the following steps:
(1) gather, extract: from plant intermediate acquisition Herba Alii fistulosi, Radix Glycyrrhizae, Herba Portulacae, Pericarpium Zanthoxyli, be then crushed to 3-with plant pulverizer
The coarse powder of 6mm minces, and is minced by coarse powder by soak with ethanol 2-3 that concentration is 75-85% month, extracts supernatant standby, i.e. kill
The plant source extracting solution of microbial inoculum；
(2) distilling, separate: put into by solidago canadesis in boiler, add water in pot dipped solidago canadesis, the bottom of a pan
Heating, steam obtains the liquid mixture of water and quintessence oil in pot top gooseneck imports condenser, obtains after oil-water separation
Solidago canadesis volatile oil；
(3) mix, grind: in the antibacterial plant source extracting solution that step (1) obtains, add the Canada one that step (2) obtains
Branch Hemerocallis citrina Baroni volatile oil and Bitertanol, to be mixed uniformly after proportionally add solvent and cosolvent and dissolve, mixing
Uniformly, then grind 20-35 minute with colloid mill, continue after adding antifreezing agent to grind 20-35 minute on colloid mill, killed
Microbial inoculum colloid；
(4) stirring: antibacterial colloid ground for step (3) is moved in emulsifying kettle, adds distilled water to antibacterial colloid full dose
2/3, under the rotating speed of 500-12000 rev/min stir 20-30 minute, i.e. obtain the aqueous emulsion of antibacterial.
The preparation method of a kind of antibacterial the most according to claim 6, it is characterised in that: in step (4), described stirring
Method is as follows: move in emulsifying kettle by antibacterial colloid ground in step (3), adds distilled water to antibacterial colloid full dose
2/3, stir 25 minutes under the rotating speed of 6000 revs/min, i.e. obtain the aqueous emulsion of antibacterial.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610650588.4A CN106234467A (en) | 2016-08-10 | 2016-08-10 | A kind of antibacterial and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610650588.4A CN106234467A (en) | 2016-08-10 | 2016-08-10 | A kind of antibacterial and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106234467A true CN106234467A (en) | 2016-12-21 |
Family
ID=58077983
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610650588.4A Pending CN106234467A (en) | 2016-08-10 | 2016-08-10 | A kind of antibacterial and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106234467A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106614805A (en) * | 2016-12-23 | 2017-05-10 | 芜湖福民生物药业股份有限公司 | Validamycin compound for preventing and treating rice sheath blight disease and preparation method thereof |
| CN106614804A (en) * | 2016-12-23 | 2017-05-10 | 芜湖福民生物药业股份有限公司 | Validamycin composition for controlling rice sheath blight disease and preparation method thereof |
| CN106665680A (en) * | 2016-12-23 | 2017-05-17 | 芜湖福民生物药业股份有限公司 | Validamycin composition for preventing and controlling rice sheath blight disease and preparation method of validamycin composition |
| CN106818921A (en) * | 2016-12-23 | 2017-06-13 | 芜湖福民生物药业股份有限公司 | Validamycin composition and preparation method thereof for preventing and treating rice sheath blight disease |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102763689A (en) * | 2012-07-17 | 2012-11-07 | 杭州师范大学 | Composite bactericide containing solidago canadensis extract |
| CN104206454A (en) * | 2014-08-26 | 2014-12-17 | 青岛保利康新材料有限公司 | Pesticide for preventing and controlling downy mildew of crops |
-
2016
- 2016-08-10 CN CN201610650588.4A patent/CN106234467A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102763689A (en) * | 2012-07-17 | 2012-11-07 | 杭州师范大学 | Composite bactericide containing solidago canadensis extract |
| CN104206454A (en) * | 2014-08-26 | 2014-12-17 | 青岛保利康新材料有限公司 | Pesticide for preventing and controlling downy mildew of crops |
Non-Patent Citations (7)
| Title |
|---|
| 代名等: "《农药安全使用新技术》", 30 September 1999, 延边人民出版社 * |
| 樊金萍等: "《家庭实用养花手册》", 31 January 2015, 金盾出版社 * |
| 潘文亮等: "《生物农药使用技术百问百答》", 31 January 2009, 中国农业出版社 * |
| 王开金等: ""加拿大一枝黄花精油的化学成分及其抗菌活性"", 《植物资源与环境学报》 * |
| 范志宏等: "《植物源万寿菊杀菌素研究及应用》", 31 July 2011, 中国农业科学技术出版社 * |
| 郑裕国等: "《生物加工过程与设备》", 31 July 2004, 化学工业出版社 * |
| 郭玉人等: "《农药安全使用技术指南》", 31 May 2012, 中国农业出版社 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106614805A (en) * | 2016-12-23 | 2017-05-10 | 芜湖福民生物药业股份有限公司 | Validamycin compound for preventing and treating rice sheath blight disease and preparation method thereof |
| CN106614804A (en) * | 2016-12-23 | 2017-05-10 | 芜湖福民生物药业股份有限公司 | Validamycin composition for controlling rice sheath blight disease and preparation method thereof |
| CN106665680A (en) * | 2016-12-23 | 2017-05-17 | 芜湖福民生物药业股份有限公司 | Validamycin composition for preventing and controlling rice sheath blight disease and preparation method of validamycin composition |
| CN106818921A (en) * | 2016-12-23 | 2017-06-13 | 芜湖福民生物药业股份有限公司 | Validamycin composition and preparation method thereof for preventing and treating rice sheath blight disease |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Amadioha | Controlling rice blast in vitro and in vivo with extracts of Azadirachta indica | |
| Kathiresan | Allelopathic potential of native plants against water hyacinth | |
| CN102217661B (en) | Botanical bactericide and preparation method and application thereof | |
| CN103931686A (en) | Plant pesticide as well as preparation method and application thereof | |
| CN104255829B (en) | Armstrong botanical fungicide and preparation method thereof | |
| CN106259516A (en) | A kind of acaricide and preparation method thereof | |
| CN106234467A (en) | A kind of antibacterial and preparation method thereof | |
| CN103229786B (en) | Complex organic plant pesticide and preparation method thereof | |
| CN106490057A (en) | A kind of composite pesticide for Rice Cropping and preparation method thereof | |
| CN104904759A (en) | Pollution-free pesticide comprising traditional Chinese medicine component | |
| CN106478275A (en) | A kind of composite pesticide of preventing and treating tobacco diseases and preparation method thereof | |
| CN103444729A (en) | Bactericidal pharmaceutical composition and applications thereof | |
| CN105052951A (en) | Seed treatment agent | |
| CN105454322A (en) | Pesticide for preventing and treating plant fungus disease | |
| CN104872204A (en) | Vegetable bactericide capable of preventing and treating phytophthora root rot of vegetables and preparation method of vegetable bactericide | |
| CN100355345C (en) | Traditional Chinese medicine composite biological pesticide using crusta as main material | |
| CN108244158A (en) | A kind of disinfectant use in agriculture and preparation method thereof | |
| CN110786344A (en) | Method for preventing and treating downy mildew of fruits and vegetables by utilizing baeckea frutescens branch and leaf extracting solution | |
| CN105284945A (en) | Method for controlling sugarcane pineapple disease | |
| CN108812707A (en) | One kind killing thrips plant essential oil composition, preparation and its application | |
| CN106490048A (en) | A kind of environment friendly agricultural of preventing and treating brassicaceous vegetable pest and disease damage and preparation method thereof | |
| Maheshwari et al. | Effectiveness of botanicals, inorganic salts and fungicide against Fusarium wilt of muskmelon under hot arid region of Rajasthan | |
| CN1093377C (en) | Composite plant extract liquid as agricultural disinfectant | |
| Nweke et al. | Effect of extracts from leaves, bark and root of Azadiratcha indica L on the vegetative growth of Colletrichum gloeosporoides: field soft rot pathogen of soursop (Annona muricata L) fruit | |
| CN105542783A (en) | Humic acid type inorganic water-retaining agent by using edible vinegar factory waste liquid and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20161221 |