CN104604861A - Plant-source synergistic compound bactericide containing resveratrol and preparation method thereof - Google Patents
Plant-source synergistic compound bactericide containing resveratrol and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a pant-source synergistic compound bactericide containing resveratrol and a preparation method thereof. The pant-source synergistic compound bactericide is prepared by compounding main components including resveratrol, acetyl ylbrit annilactone and neocnidilide and an addition agent, and the preparation form is environment-friendly missible oil, a microemulsion, an aqueous solution or dissoluble liquid. The preparation contains 0.5-20 percent of resveratrol, 0.1-15 percent of acetyl ylbrit annilactone, 0.1-20 percent of neocnidilide and the balance of the addition agent. Compared with a chemical bactericide, the pant-source bactericide has the advantages of being more in acting sites, long in drug duration and fast in degradation velocity, does not easily develop resistance to drugs, and is less pollution to environment. The pant-source synergistic compound bactericide can prevent and cure agricultural diseases such as gray mold, anthracnose, downy mildew, phytophthora root rot and brown rot on melons, fruits, vegetables and commercial crops and has a wide application prospect and development value.
Description
Technical field
The invention belongs to technical field of pesticide; a kind of with resveratrol, acetyl inula Britannica lactone and neocindilide three Plants source material bactericide that is active component and preparation method thereof specifically; can be used for the multiple diseases of preventing and treating in agricultural, belong to botanical fungicide field.
Background technology
Chemical bactericide is used in modern agriculture fast in a large number because of efficient, China's year spray liquid more than 100,000,000 ten thousand tons, the area of chemical control more than 2,800,000,000 hectares times/year, and in ascendant trend year by year.Due to factors such as the high poison of some chemical pesticides, high residue and unreasonable uses, while bringing agricultural high yield, also bring the series of negative effect such as threat of the pollution of environment, the exceeding standard of Practice for Pesticide Residue in Agricultural Products amount, ecological disruption, the drug-fast generation of damage by disease and insect and personal safety, be unfavorable for the sustainable development of agricultural.
Botanical fungicide derives from natural plants because of its active ingredient, in crop disease control, have environmental friendliness, to person poultry safety, disease such as not easily to develop immunity to drugs at the feature, be the bactericide kind that production non-polluted farm product should preferentially be selected, be therefore also subject to various countries and pay attention to.From natural resources treasure-house, find Fungicidal active substance, become important channel and the study hotspot of new type bactericide initiative.After particularly composite by the science of multiple components, the effect of plant resource Fungicidal active substance can significantly improve.
Resveratrol (Resveratrol) is polyphenol compound, is mainly derived from the plants such as grape (claret), giant knotweed, peanut, mulberry fruit.Resveratrol is a kind of biological very strong natural polyphenol class material, is also called resvertrol, has anti-bacteria and anti-virus, improves the effect of immunity.Acetyl inula Britannica lactone is one of active component of Chinese medicine Inula britannica chinensis, reports after deliberation, has sterilization, antiviral isoreactivity effect.Neocindilide (neocnidilide) is mainly derived from caryophyllus oil, cinnamon leaves oil, cassia oil, camphorated oil, nutmeg wet goods, has good therapeutic action for downy mildew and grey mold, but is limited in scope to other plant disease control.
By studying physicochemical property and the biological property of three, science of carrying out is composite, and then obtains a kind of novel plant source synergistic complex bactericide, under the prerequisite of feature retaining each active component, improves control efficiency, expands control spectrum.
According to data-searching, have no open report so far about patent on phytopathogen of resveratrol, acetyl inula Britannica lactone and neocindilide composite usage and product at present.
Summary of the invention
The object of the invention is to overcome above-mentioned the deficiencies in the prior art; a kind of preparation method being Fungicidal active substance and synergism compound preparation thereof with botanical components resveratrol, acetyl inula Britannica lactone and neocindilide is provided, is mainly used in control melon, the agricultural disease such as powdery mildew, gray mold, anthracnose, downy mildew, phytophthora root rot, brown rot really, on vegetables and other economic crops.
In order to realize above-mentioned task, the present invention takes following technical solution to be achieved:
A kind of Botanical synergistic compound bactericide containing resveratrol and preparation method thereof, its feature is, this obtained Botanical synergistic compound bactericide is main component by resveratrol, acetyl inula Britannica lactone and neocindilide and is added additive compound to form, and dosage form is environment-friendly type missible oil, microemulsion or aqueous emulsion; Wherein:
Described environment-friendly type missible oil is prepared by following raw materials by weight:
Resveratrol: 0.5 ~ 20%, acetyl inula Britannica lactone: 0.1 ~ 15%, neocindilide 0.1% ~ 20%, emulsifier: 5 ~ 10%, synergist: 0 ~ 20%, surplus is organic solvent;
Described microemulsion is prepared by following raw materials by weight:
Resveratrol: 0.5 ~ 20%, acetyl inula Britannica lactone: 0.1 ~ 15%, neocindilide 0.1% ~ 20%, organic solvent: 0 ~ 10%, surfactant: 5 ~ 10%, synergist: 0 ~ 5%, antifreezing agent: 4 ~ 10%, surplus is water;
Described aqua is prepared by following raw materials by weight:
Resveratrol: 0.5 ~ 20%, acetyl inula Britannica lactone: 0.1 ~ 15%, neocindilide 0.1% ~ 20%, organic solvent: 0 ~ 10%, surfactant: 5 ~ 10%, synergist: 0 ~ 10%, antifreezing agent: 0 ~ 15%, surplus is water;
Described soluble liquid is prepared by following raw materials by weight:
Resveratrol: 0.5 ~ 20%, acetyl inula Britannica lactone: 0.3 ~ 15%, neocindilide 0.5% ~ 20%, surfactant: 5 ~ 20%, synergist: 5 ~ 15%, surplus is organic solvent.
Described organic solvent is the mixture of one or more in ethyl acetate, cyclohexanone, methyl oleate, ethanol, methyl alcohol, acetone, dimethyl formamide;
Described emulsifier is the mixture of one or more in alkyl benzene sulfonate, alkylphenol polyoxyethylene class, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers and similar substance thereof, this Pan's series, tween;
Described surfactant is one or more mixture in alkyl benzene sulfonate, alkylphenol polyoxyethylene class, phenethyl phenol polyethenoxy ethers, the addition product of phenethyl phenol polyethenoxy ether polyoxypropylene ethers and similar substance, castor oil and oxirane, aralkyl phenol polyethenoxy ether formaldehyde condensation products;
Described synergist is the mixture of one or more in organosilicon, Butacide, Octacide 264, alkyl carbonate, azone;
Described antifreezing agent is ethylene glycol or propane diols;
Described thickener is the mixture of one or more in polyvinyl alcohol, xanthans, sodium carboxymethylcellulose, Magnesiumaluminumsilicate.
The preparation method of above-mentioned Botanical synergistic compound bactericide, its feature is:
The preparation method of described environment-friendly type missible oil is: according to formula ratio by resveratrol, acetyl inula Britannica lactone, neocindilide, emulsifier, organic solvent, synergist mixing, stir in a kettle., get product through quality inspection;
The preparation method of described microemulsion is: resveratrol, acetyl inula Britannica lactone, neocindilide, organic solvent, surfactant, synergist, antifreezing agent and water are mixed according to formula ratio, stir transparent in a kettle., get product through quality inspection;
The preparation method of described aqueous emulsion is: resveratrol, acetyl inula Britannica lactone, neocindilide, organic solvent, surfactant, synergist, antifreezing agent, thickener and water are mixed according to formula ratio; in the emulsified dispersion of the effect of high-speed shearing machine; after quality inspection, make finished product.
Botanical synergistic compound bactericide of the present invention is a kind of novel bactericide, may be used for preventing and treating melon, the agricultural disease such as powdery mildew, gray mold, anthracnose, downy mildew, phytophthora root rot, brown rot really, on vegetables and other economic crops, there is good control efficiency, on organic farm products is produced, have very big application and popularization value, be desirable novel non-pollution bactericide.
Compared with prior art, advantage of the present invention is as follows:
1. resveratrol, acetyl inula Britannica lactone, neocindilide are natural active matter, easily degrade in the environment, and to mammal low toxicities such as people and animals, to pest natural enemy and other beneficial organism safety, and germ not easily develops immunity to drugs to it; After rationally composite, improve control efficiency, expand control spectrum.
2. adopted formulation is mainly environment-friendly type missible oil, microemulsion, aqua etc., without aromatic hydrocarbon solvents such as dimethylbenzene, greatly reduces the destruction to environment, reduces the murder by poisoning to operator in dispenser process.
3. photostability raising, lasting period are suitable for, and can reduce dispenser frequency and cost accounting, and be conducive to alleviating the pressure to environment further.
4. the economic crops disease such as pair powdery mildew, gray mold, anthracnose, downy mildew, phytophthora root rot, brown rot has good control efficiency, can obtain larger economy, society and ecological benefits.
Embodiment
Botanical synergistic compound bactericide of the present invention; with resveratrol, acetyl inula Britannica lactone, neocindilide for active component; add a certain proportion of solvent and cosolvent and other insecticides adjuvant; carry out formulation, environment-friendly type missible oil, microemulsion, aqua and soluble liquid can be obtained.Wherein, resveratrol consumption by weight percentage 0.5 ~ 20%, acetyl inula Britannica lactone consumption by weight percentage 0.1 ~ 15%, neocindilide consumption by weight percentage 0.1 ~ 20%, all the other are auxiliary agents.
In Botanical synergistic compound bactericide of the present invention; resveratrol; acetyl inula Britannica lactone and neocindilide; part by weight is between 5:1:1 ~ 1:1:4 be suitable; dosage form is environment-friendly type missible oil, microemulsion or aqueous emulsion, is mainly used in economic crops diseases such as preventing and treating powdery mildew, gray mold, anthracnose, downy mildew, phytophthora root rot, brown rot.
1. environment-friendly type missible oil: resveratrol: 0.5 ~ 20%, acetyl inula Britannica lactone: 0.1 ~ 15%, neocindilide 0.1% ~ 20%, all the other are auxiliary agents, and auxiliary agent comprises: emulsifier, organic solvent, synergist.
Emulsifier: 5 ~ 10% is the mixture of one or more in alkyl benzene sulfonate, alkylphenol polyoxyethylene class, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers and similar substance thereof, Well201, Well205, M30, this Pan's series, tween;
Synergist: 0 ~ 20% is the mixture of one or more in organosilicon, Butacide, Octacide 264, alkyl carbonate, azone;
Surplus is organic solvent together to 100%: be the mixture of one or more in ethyl acetate, acetone, ethanol.
2. microemulsion, resveratrol: 0.5 ~ 20%, acetyl inula Britannica lactone: 0.1 ~ 15%, neocindilide 0.1% ~ 20%, all the other are auxiliary agents, and auxiliary agent comprises: surfactant, organic solvent, synergist, antifreezing agent, water.
Surfactant: 5 ~ 10% is one or more mixture in the addition product of alkyl benzene sulfonate, alkylphenol polyoxyethylene class, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers and similar substance, castor oil and oxirane, aralkyl phenol polyethenoxy ether formaldehyde condensation products;
Organic solvent: 0 ~ 10% is one or more mixture in methyl alcohol, cyclohexanone, dimethyl formamide, dimethyl sulfoxide (DMSO), ethyl acetate, acetone, ethanol;
Synergist: 0 ~ 5% is the mixture of one or more in organosilicon, Butacide, Octacide 264, alkyl carbonate, azone;
Antifreezing agent: 4 ~ 10% can be a kind of in ethylene glycol, propane diols;
Surplus use water polishing to 100%.
3. aqua: resveratrol: 0.5 ~ 20%, acetyl inula Britannica lactone: 0.1 ~ 15%, neocindilide 0.1% ~ 20%, all the other are auxiliary agents, and auxiliary agent comprises: surfactant, organic solvent, synergist, antifreezing agent and water.
Surfactant: 5 ~ 10% is one or more mixture in the addition product of alkyl phosphate class, alkylphenol polyoxyethylene phosphoric acid ester, aralkyl phenol polyethenoxy ether phosphoric acid ester, aliphatic acid polyethenoxy ether class, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers and analog, castor oil and oxirane, aralkyl phenol polyethenoxy ether formaldehyde condensation products;
Organic solvent: 0 ~ 10% is one or more mixture in methyl alcohol, cyclohexanone, dimethyl formamide, dimethyl sulfoxide (DMSO), ethyl acetate, acetone, ethanol;
Synergist: 0 ~ 10% is the mixture of one or more in organosilicon, Butacide, Octacide 264, alkyl carbonate, azone;
Antifreezing agent: 0 ~ 15% is any one of ethylene glycol or propane diols;
Surplus use water polishing to 100%.
4. soluble liquid: resveratrol: 0.5 ~ 20%, acetyl inula Britannica lactone: 0.1 ~ 15%, neocindilide 0.1% ~ 20%, all the other are auxiliary agents, and auxiliary agent comprises: surfactant, organic solvent, synergist and organic solvent.
Surfactant: 5 ~ 20% is one or more mixture in the addition product of alkyl phosphate class, alkylphenol polyoxyethylene phosphoric acid ester, aralkyl phenol polyethenoxy ether phosphoric acid ester, aliphatic acid polyethenoxy ether class, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers and analog, castor oil and oxirane, aralkyl phenol polyethenoxy ether formaldehyde condensation products;
Synergist: 5 ~ 15% is the mixture of one or more in organosilicon, Butacide, Octacide 264, alkyl carbonate, azone;
Organic solvent: be one or more mixture polishing to 100% in methyl alcohol, cyclohexanone, dimethyl formamide, dimethyl sulfoxide (DMSO), ethyl acetate, acetone, ethanol;
The production method of above-mentioned Botanical synergistic compound bactericide environment-friendly type missible oil is: raw material is weighed medicament by formula, adds mixing successively, and stir in a kettle., after quality inspection, finally make finished product.
The production method of above-mentioned Botanical synergistic compound bactericide microemulsion is: weighed by composition of raw materials, add mixing successively, stir transparent in a kettle., after quality inspection, finally make finished product.
The production method of above-mentioned Botanical synergistic compound bactericide aqua is: weighed by formula by raw material, add mixing successively, stir transparent in a kettle., after quality inspection, finally make finished product.
The production method of above-mentioned Botanical synergistic compound bactericide soluble liquid is: weighed by formula by raw material, add mixing successively, stir transparent in a kettle., after quality inspection, finally make finished product.
Embodiment
In order to better understand essence of an invention, describe the technology contents of invention below in detail by embodiment, but the content of invention is not limited to these embodiments.
Embodiment 1:0.5% resveratrol environment-friendly type missible oil is prepared
At room temperature, get 0.5 part of resveratrol, 0.1 part of acetyl inula Britannica lactone, 0.1 part of neocindilide, (resveratrol, acetyl inula Britannica lactone, the composite ratio of neocindilide is 5:1:1), said mixture basis adds 6 parts of organosilicons successively, 4 parts of neopelexes and 1 part of OPEO and 1 part of phenethyl phenol polyethenoxy polyethenoxy ether, 100 parts are complemented to ethanol+ethyl acetate+dimethyl formamide (6:3.5:0.5), stir 10 ~ 30 minutes with mixing speed 100 ~ 200 revs/min, 0.5% resveratrol environment-friendly type missible oil can be obtained.
Embodiment 2:5% resveratrol aqua is prepared
At room temperature; get 5 parts of resveratrols; 5 parts of acetyl inula Britannica lactones; 20 parts of neocindilides; (resveratrol; acetyl inula Britannica lactone; the composite ratio of neocindilide is 1:1:4); 1 part of organosilicon is added successively, 5 parts of polyoxyethylene nonylphenol ether, thermally coupled distillation columns 3 parts in said mixture; glycerine 4 parts; mix rear solution polishing to 100 part, stir 10 ~ 30 minutes with mixing speed 100 ~ 200 revs/min, 5% resveratrol aqua can be obtained.
Embodiment 3:6% resveratrol microemulsion is prepared
At room temperature; get 6 parts of resveratrols; 15 parts of acetyl inula Britannica lactones; 3 parts of neocindilides; (resveratrol; acetyl inula Britannica lactone; the composite ratio of neocindilide is 2:5:1); then in said mixture, add 2 parts of organosilicons, 6 parts of calcium dodecyl benzene sulfonates, 3 parts of styrenated phenol polyoxyethylene (n30) ethers, 2 parts of ethylene glycol successively; water polishing to 100 part; stir 10 ~ 30 minutes with mixing speed 100 ~ 200 revs/min after mixing, 6% resveratrol microemulsion can be obtained.
Embodiment 4:10% resveratrol microemulsion is prepared
At room temperature; get 10 parts of resveratrols; 15 parts of acetyl inula Britannica lactones; 5 parts of neocindilides; (resveratrol; acetyl inula Britannica lactone; the composite ratio of neocindilide is 2:3:1); then in said mixture, add 1 part of organosilicon, 2 parts of calcium dodecyl benzene sulfonates, 1 part of styrenated phenol polyoxyethylene (n30) ether, 1 part of ethylene glycol successively; water polishing to 100 part, stirs 10 ~ 30 minutes with mixing speed 100 ~ 200 revs/min after mixing, can obtain 10% resveratrol microemulsion.
Embodiment 5:20% resveratrol soluble liquid is prepared
At room temperature; get 20 parts of resveratrols, 4 parts of acetyl inula Britannica lactones, 4 parts of neocindilides; (resveratrol; acetyl inula Britannica lactone, the composite ratio of neocindilide is 5:1:1), in said mixture, add 5 parts of polyoxyethylene nonylphenol ether successively; thermally coupled distillation columns 3 parts; glycerine 4 parts of organic solvent polishings to 100 part, stir 10 ~ 30 minutes with mixing speed 100 ~ 200 revs/min after mixing, can obtain 20% resveratrol soluble liquid.
Embodiment 6: five Plants source synergistic complex bactericide is tested the indoor biometrics of several germ
This experiment, in vitro, determines five Plants source synergistic complex bactericide to the inhibit activities of phytopathogen, experimental technique and result of the test as follows:
6.1 reagent agent
0.5% resveratrol missible oil, medicament code name B1;
5% resveratrol aqua, medicament code name B2;
6% resveratrol microemulsion, medicament code name B3;
10% resveratrol microemulsion, medicament code name B4;
20% resveratrol soluble concentrate, medicament code name B5;
6.2 for examination bacterial classification
Cucumber anthracnose, cucumber downy mildew, graw mold of tomato, early blight of tomato, tomato late blight, provide by Fuji Biotechnology Development (Shanghai) Co., Ltd..
6.3 test method
Mycelial growth rate method is adopted to test, according to formulae discovery inhibiting rate in vitro in above-mentioned five Plants source synergistic complex bactericide:
Mycelial growth inhibition rate=
× 100%
6.4 result of the test
Determine above-mentioned five Plants source synergistic complex bactericide to the virulence for examination pathogen with growth rate method, thus obtain its extract to the virulence for examination pathogen, the results are shown in Table 1 ~ 5
Table 1 B1 Botanical synergistic compound bactericide suppresses the virulence (72h) of mycelial growth
Table 2 B2 Botanical synergistic compound bactericide suppresses the virulence (72h) of mycelial growth
Table 3 B3 Botanical synergistic compound bactericide suppresses the virulence (72h) of mycelial growth
Table 4 B4 Botanical synergistic compound bactericide suppresses the virulence (72h) of mycelial growth
Table 5 B5 Botanical synergistic compound bactericide suppresses the virulence (72h) of mycelial growth
The field control effectiveness test of embodiment 7: five Plants source synergistic complex bactericide bacterium of downy mildew of cucumber
For the field efficacy situation of clear and definite 5 Plants source synergistic complex bactericide, in Xinle county of Hebei province, the drug effect test of field zone preventing and treating cucumber downy mildew is carried out to this medicament.
7.1 reagent agent
0.5% resveratrol missible oil, medicament code name B1;
5% resveratrol aqua, medicament code name B2;
6% resveratrol microemulsion, medicament code name B3;
10% resveratrol microemulsion, medicament code name B4;
20% resveratrol soluble concentrate, medicament code name B5;
75% tpn wetting powder, University of Science and Technology insecticide factory of Xinyi City of Zhejiang Province.
7.2 test article
Syringe (10ml) 3, knapsack sprayer two, drop (300), Enamel jar (1000ml) 1,3,1000ml graduated cylinder, 2,100ml graduated cylinder, pencil 3, minute book 1, and one, camera.
7.3 for studying thing
Cucumber.
7.4 experimental scheme and method
Small plot experiment is randomized arrangement, and repeat 3 times, plot area is depending on actual conditions.It is 500 that reagent agent is diluted to concentration respectively by the content of active ingredient, 250,125 μ g/ml carry out blade face conventional spray (namely blade face occurring that water membrane is advisable), and 75% tpn wetting powder 1000 times liquid carries out blade face conventional spray for contrasting medicament, and establishes clear water to contrast.Investigate according to the classification of blank incidence.Adopt following stage division:
0 grade: anosis;
1 grade: lesion area accounts for less than 5% of one-piece blade area;
3 grades: lesion area accounts for 6% ~ 15% of one-piece blade area;
5 grades: lesion area accounts for 16 ~ 25% of one-piece blade area;
7 grades: lesion area accounts for 26 ~ 50% of one-piece blade area;
9 grades: lesion area accounts for more than 50% of one-piece blade area;
According to survey data, calculate disease index and the control efficiency of each process.
Disease index is pressed formula (1) and is calculated, and result of calculation retains 2 significant digits:
X = ∑（N
i×i）/ (N×9)×100% ┈┈（1）
In formula:
X-----disease index;
N
i-----sick number of sheets at different levels;
I-----relative level numerical value;
N-----investigates total number of sheets.
Control efficiency is pressed formula (2) and is calculated:
P = (CK- PT)/ CK ×100% ┈┈（2）
In formula:
P----control efficiency, unit is percentage (%);
CK----blank disease index;
PT----chemicals treatment disease index;
7.5 result of the tests and statistics
Adopt conventional spray-on process to test the field efficacy of 5 Plants source synergistic complex agent to cucumber downy mildew, the results are shown in Table 1.
Table 15 Plants source bactericide is to the field control effectiveness test result of cucumber downy mildew
Note: in " preventive effect " hurdle, marks identical lowercase person and represents in variance analysis (DMRT method) after ordered series of numbers, without significant difference in 5% level, lower same.
As can be seen from the above table, when 5 Plants source medicament active ingredient concentration are 500 μ g/ml, preventive effect, all more than 80%, contrasts the control efficiency of 75% tpn wetting powder, 1000 times of liquid without significant difference with medicament.When active ingredient concentration is 125 μ g/ml, the preventive effect of 5 kinds of medicaments is all more than 60%.In general, 5 Plants source synergistic complex medicaments are all better to the control efficiency of cucumber downy mildew.
Embodiment 8:5 Plants source synergistic complex bactericidal agent for preventing and treating tomato blight field control effectiveness test
Carried out the drug effect test of field zone of 5 Plants source synergistic complex bactericidal agent for preventing and treating tomato blights in Baoshan District, Shanghai, experimental scheme is with embodiment 7, and contrast medicament is 24% RH-7592 suspending agent, and test of pesticide effectiveness result is as table 2:
Table 25 Plants source bactericide is to the field control effectiveness test result of tomato blight
As can be seen from the above table, when 5 Plants source medicament active ingredient concentration are 800 μ g/ml, preventive effect, all more than 75%, and contrasts the control efficiency of 24% RH-7592,1000 times of liquid without significant difference.
Embodiment 9:5 Plants source synergistic complex bactericidal agent for preventing and treating bitter rot or anthracnose of grape field control effectiveness test
Carried out the drug effect test of field zone of 5 Plants source synergistic complex bactericidal agent for preventing and treating bitter rot or anthracnose of grapes in Yantai, Shandong, experimental scheme is with embodiment 7, and contrast medicament is 80% carbendazol wettable powder, 1000 times of liquid, and test of pesticide effectiveness result is as table 3:
Table 3 botanical fungicide is to the field control effectiveness test result of bitter rot or anthracnose of grape
As can be seen from the above table, when 5 Plants source medicament active ingredient concentration are 500 μ g/ml, preventive effect, all more than 80%, contrasts the control efficiency of 80% carbendazol wettable powder 1000 times without significant difference with medicament.When active ingredient concentration is 250 μ g/ml, the preventive effect of 5 kinds of medicaments is all more than 60%.In general, 5 Plants source medicaments are good to the control efficiency of bitter rot or anthracnose of grape compared with other medicaments.
The field experiment result of comprehensive above three tables, 5 Plants source bactericides, in field trial, all show stronger bacteriostatic activity, and some medicament and chemical agent bacteriostasis property are without significant difference.Analyze reason, this may be determined by the constituent of active substance of plant complexity, and the essence of effect is just because of result interactional between Multiple components.In addition, botanical fungicide has certain lasting period, and to non-target organism and environmental friendliness, therefore very applicable in economic crops and Organic Food Development, there is very large application and popularization value.
Claims (5)
1. Botanical synergistic compound bactericide containing resveratrol and preparation method thereof, it is characterized in that, the form of this bactericide is environment-friendly type missible oil, microemulsion, aqua or soluble liquid; Wherein:
Described environment-friendly type missible oil is prepared by following raw materials by weight:
Resveratrol: 0.5 ~ 20%, acetyl inula Britannica lactone: 0.1 ~ 15%, neocindilide 0.1% ~ 20%, emulsifier: 5 ~ 10%, synergist: 0 ~ 20%, surplus is organic solvent;
Described microemulsion is prepared by following raw materials by weight:
Resveratrol: 0.5 ~ 20%, acetyl inula Britannica lactone: 0.1 ~ 15%, neocindilide 0.1% ~ 20%, organic solvent: 0 ~ 10%, surfactant: 5 ~ 10%, synergist: 0 ~ 5%, antifreezing agent: 4 ~ 10%, surplus is water;
Described aqua is prepared by following raw materials by weight:
Resveratrol: 0.5 ~ 20%, acetyl inula Britannica lactone: 0.1 ~ 15%, neocindilide 0.1% ~ 20%, organic solvent: 0 ~ 10%, surfactant: 5 ~ 10%, synergist: 0 ~ 10%, antifreezing agent: 0 ~ 15%, surplus is water;
Described soluble liquid is prepared by following raw materials by weight:
Resveratrol: 0.5 ~ 20%, acetyl inula Britannica lactone: 0.1 ~ 15%, neocindilide 0.1% ~ 20%, surfactant: 5 ~ 20%, synergist: 5 ~ 15%, surplus is organic solvent.
2. a kind of Botanical synergistic compound bactericide containing resveratrol and preparation method thereof as claimed in claim 1, is characterized in that:
Described organic solvent is the mixture of one or more in ethyl acetate, cyclohexanone, methyl oleate, ethanol, methyl alcohol, acetone, dimethyl formamide;
Described emulsifier is the mixture of one or more in alkyl benzene sulfonate, alkylphenol polyoxyethylene class, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers and similar substance thereof, Well201, Well205, M30, this Pan's series, tween;
Described surfactant is one or more mixture in alkyl benzene sulfonate, alkylphenol polyoxyethylene class, phenethyl phenol polyethenoxy ethers, the addition product of phenethyl phenol polyethenoxy ether polyoxypropylene ethers and similar substance, castor oil and oxirane, aralkyl phenol polyethenoxy ether formaldehyde condensation products;
Described synergist is the mixture of one or more in organosilicon, Butacide, Octacide 264, alkyl carbonate, azone;
Described antifreezing agent is ethylene glycol or propane diols;
Described thickener is the mixture of one or more in polyvinyl alcohol, xanthans, sodium carboxymethylcellulose, Magnesiumaluminumsilicate.
3. the Botanical synergistic compound bactericide described in claim 1 or 2, is characterized in that, described resveratrol, and the part by weight of acetyl inula Britannica lactone and neocindilide is 5:1:1 ~ 1:1:4.
4. a kind of Botanical synergistic compound bactericide containing resveratrol according to claim 1 and preparation method thereof, is characterized in that:
The preparation method of described environment-friendly type missible oil is: according to formula ratio by resveratrol, acetyl inula Britannica lactone, neocindilide, emulsifier, organic solvent, synergist mixing, stir in a kettle., get product;
The preparation method of described microemulsion is: resveratrol, acetyl inula Britannica lactone, neocindilide, organic solvent, surfactant, synergist, antifreezing agent and water are mixed according to formula ratio, stir transparent in a kettle., get product;
The preparation method of described aqua is: resveratrol, acetyl inula Britannica lactone, neocindilide, organic solvent, surfactant, synergist, antifreezing agent and water are mixed according to formula ratio, stir transparent in a kettle., get product;
The preparation method of described soluble liquid is: resveratrol, acetyl inula Britannica lactone, neocindilide, surfactant, synergist and organic solvent are mixed according to formula ratio, stir transparent in a kettle., get product.
5. the arbitrary Botanical synergistic compound bactericide described in claim 1-3 is in the application preventing and treating powdery mildew, gray mold, anthracnose, downy mildew, phytophthora root rot, brown rot agricultural disease.
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| CN105360113A (en) * | 2015-12-04 | 2016-03-02 | 兰州交通大学 | Preparation method for synergistic pesticide microemulsion |
| CN106116916A (en) * | 2016-06-17 | 2016-11-16 | 广西宏华生物实业股份有限公司 | A kind of biological medicine fertilizer and its preparation method and application |
| CN108077272A (en) * | 2018-02-02 | 2018-05-29 | 中国农业大学 | Synergetic effect additive application during preventing grape grey mould of the resveratrol as pyrimethanil |
| CN108124890A (en) * | 2017-12-29 | 2018-06-08 | 杨凌馥稷生物科技有限公司 | A kind of Pesticidal combination containing jervine |
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| CN112336715A (en) * | 2020-11-06 | 2021-02-09 | 青岛嘉智生物技术有限公司 | Application of inula britannica extract in preparation of medicine for treating swine pleuropneumonia actinobacillus |
| CN112442375A (en) * | 2020-12-02 | 2021-03-05 | 山东省农业科学院农业资源与环境研究所 | Root rot resistant water-retaining multifunctional soil conditioner and preparation method thereof |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105360113A (en) * | 2015-12-04 | 2016-03-02 | 兰州交通大学 | Preparation method for synergistic pesticide microemulsion |
| CN106116916A (en) * | 2016-06-17 | 2016-11-16 | 广西宏华生物实业股份有限公司 | A kind of biological medicine fertilizer and its preparation method and application |
| CN108124890A (en) * | 2017-12-29 | 2018-06-08 | 杨凌馥稷生物科技有限公司 | A kind of Pesticidal combination containing jervine |
| CN108124890B (en) * | 2017-12-29 | 2020-04-10 | 杨凌馥稷生物科技有限公司 | Insecticidal composition containing veratrine |
| CN108077272A (en) * | 2018-02-02 | 2018-05-29 | 中国农业大学 | Synergetic effect additive application during preventing grape grey mould of the resveratrol as pyrimethanil |
| CN108739809A (en) * | 2018-06-13 | 2018-11-06 | 北京清源保生物科技有限公司 | It is a kind of prevention fungal disease bactericidal composition and its application |
| CN112336715A (en) * | 2020-11-06 | 2021-02-09 | 青岛嘉智生物技术有限公司 | Application of inula britannica extract in preparation of medicine for treating swine pleuropneumonia actinobacillus |
| CN112336715B (en) * | 2020-11-06 | 2022-11-04 | 青岛嘉智生物技术有限公司 | Application of inula britannica extract in preparation of medicine for treating swine pleuropneumonia actinobacillus |
| CN112442375A (en) * | 2020-12-02 | 2021-03-05 | 山东省农业科学院农业资源与环境研究所 | Root rot resistant water-retaining multifunctional soil conditioner and preparation method thereof |
| CN112442375B (en) * | 2020-12-02 | 2021-11-09 | 山东省农业科学院农业资源与环境研究所 | Root rot resistant water-retaining multifunctional soil conditioner and preparation method thereof |
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