CN104488923B - A kind of Botanical synergistic compound bactericide and application thereof - Google Patents
A kind of Botanical synergistic compound bactericide and application thereof Download PDFInfo
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Abstract
The invention discloses a kind of Botanical synergistic compound bactericide and application thereof, this composite bactericide adds additive compound with jamaicin and Physcion form for main component, and dosage form is environment-friendly type missible oil, microemulsion, aqua or soluble liquid; Containing jamaicin 0.1% ~ 20% in preparation, Physcion 0.1% ~ 20%, all the other are auxiliary agent.The present invention is not containing any chemical pesticide composition, and be easy to degraded in the environment, the worry of non-environmental-pollution and residual hazard, to people, animal, Environmental security, germ not easily develops immunity to drugs to it; Melon, the agricultural disease such as powdery mildew, gray mold, anthracnose, downy mildew, phytophthora root rot, brown rot, moire blight really, on vegetables and tea tree can be prevented and treated, at melon, really, vegetables, tealeaves, the foreign exchange earning such as medicinal material main agricultural byproducts on there is very big application and popularization value, be desirable novel non-pollution bactericide.
Description
Technical field
The invention belongs to technical field of pesticide, specifically be active component bactericide and the application thereof of jamaicin and Physcion two Plants source material, be mainly used in control melon, the agricultural disease such as powdery mildew, gray mold, anthracnose, downy mildew, phytophthora root rot, brown rot, moire blight really, on vegetables and tea tree.
Background technology
Powdery mildew, gray mold, anthracnose, downy mildew, phytophthora root rot, brown rot, moire blight etc. be melon, really, vegetables, Major Diseases on tealeaves, damaging range is wide, and in control, difficulty is larger.The medicament mainly chemical bactericide of current these diseases of control, but chemical medications a large amount of for a long time causes the appearance creating the problems such as pesticide resistance, environmental pollution, harm humans health; In addition, the impact that the export of farm produce meets with repeatly " green barrier ", causes country to have to limit the use of many chemical pesticides.From natural resources treasure-house, find Fungicidal active substance, become important channel and the study hotspot of new type bactericide initiative.After particularly composite by the science of multiple components, the effect of plant resource Fungicidal active substance can significantly improve.
Jamaicin (berberine) is quaternary amine type isoquinoline alkaloid, is mainly present in the ranunculaceae plant coptis, rutaceae cork tree, Berberidaceae plant barberry.It prevents and treats the multiple diseases such as apple canker, ring spot, cucumber downy mildew, squash marble dust, graw mold of tomato, tealeaves moire blight, Phytophthora capsici disease all better control efficiency; Indoor biometrics and field efficacy measurement result show, jamaicin all has good control efficiency to early blight of tomato, pepper anthracnose, graw mold of tomato, powdery mildew of cucumber etc.
Physcion (physcion) is a kind of botanical pesticide extracted from the root, stem of Chinese herbal medicine polygonaceae plant rheum officinale, belongs to anthraquinone analog compound.Indoor biometrics result shows: Physcion has the bacteriostatic activity of wide spectrum, to rice blast fungus, botrytis cinerea pers and obligatory parasitic bacteria wheat powdery mildew and cucumber white powder, all there is obvious activity, also have fungistatic effect significantly to bacterium of downy mildew of cucumber simultaneously.Show have good suppression and control efficiency to powdery mildew of cucumber through toxicity mensuration and field control effectiveness test result.
According to the data-searching that applicant does, current jamaicin and Physcion is composite or mixture bactericide based on jamaicin, Physcion are composite mainly contains:
Ge Xizhen etc. apply for a patent " a kind of wetting powder and preparation method thereof preventing and treating fruits and vegetables brown rot, aphid; publication number CN103975962A ", this disclosure of the invention is a kind of prevents and treats the wetting powder and preparation method thereof of fruits and vegetables brown rot, aphid, and active ingredient is Sichuan chinaberry extract and jamaicin; Li Jies etc. apply for a patent " jamaicin insecticide and preparation method thereof, publication number CN103814916A ", the jamaicin insecticide of this invention, and its formula comprises jamaicin 18-30 part, matrine 12-28 part etc.; Wang Yingzi etc. apply for a patent " a kind of Synergistic complex biological bactericide and application thereof, publication number
CN103548849A ", this invention relates to a kind of Synergistic compound botanical source bactericide be made up of eugenol and jamaicin; Publication number is that in the application for a patent for invention " one resists jujube fruit-shrink disease emulsion and preparation method thereof strenuously high sticking " of CN103503919A, high adhesive force jujube fruit-shrink disease emulsion comprises active ingredient (streptomycin, instant boron, jamaicin), adhesive (shitosan, sodium carboxymethylcellulose CMC-NA-glycerine) and emulsifier (Tween 80); The application for a patent for invention " a kind of germ killing drugs composition and application thereof " that publication number is " CN103444729A " discloses a kind of germ killing drugs composition and application thereof, and described active compound comprises at least two kinds of active components: jamaicin and amino-oligosaccharide; The application for a patent for invention " broad-spectrum bactericide of plant source " that publication number is " CN102940000A " discloses a kind of broad-spectrum bactericide of plant source, it comprises following raw material, by weight percentage: jamaicin 0.5 ~ 4.5%, astragalus polyose 0.5 ~ 20%, Lemna paucicostata 0.5 ~ 20%, extract solution from aloe 0.5 ~ 20%, Chinese honey locust 0.5 ~ 20%, surplus are water; Wang Yingzi etc. apply for a patent " a kind of composite bactericide containing jamaicin and polyoxin and application thereof, publication number CN102894002A ", and this invention relates to a kind of Environmental compatibility, high-efficiency low-toxicity, the composite bactericide that is made up of jamaicin and polyoxin; The application for a patent for invention " jamaicin qinghaosu compound algae-inhibiting agent " that publication number is " CN102771484A " discloses a kind of jamaicin qinghaosu compound algae-inhibiting agent, and it is mainly containing jamaicin 600mg/L and qinghaosu 6-7.5mg/L; Publication number is that the application for a patent for invention " jamaicin is as the application of weed killer herbicide " of " CN102349520A " finds, berberine all has significant herbicidal effect to multiple weeds, especially with other weed killer herbicides as glyphosate or Acetochlor used in combination after, herbicidal effect is better; Publication number is in the application for a patent for invention " a kind of botanical fungicide and production method thereof " of " CN102119703A ", and bactericide is respectively 20-63%, 5-10%, 5-10%, 17-40%, 5-10%, 5-10% by weight percentage by rheum officinale, rosin, aloe, jamaicin, Chinese honey locust and chinaberry root and is pulverized and mixed rear 50-80% ethanol and leaches concentrated formulated; Publication number is in the patent of invention " a kind of botanical fungicide and application thereof " of " CN101961019A ", bactericide is two kinds of effective ingredient jamaicins and the Osthole of 1:20 ~ 20:1 by mass percent, and surfactant NP-100, penetrating agent azone, antioxidant 2,6 BHT, preservative sodium benzoate and solvent Homogeneous phase mixing are obtained; The application for a patent for invention " berberine plant source insecticide and preparation method thereof " that publication number is " CN101940229A " relates to a kind of berberine plant source insecticide and preparation method thereof, the raw material composition of this insecticide is by weight ratio: barberry 40 ~ 50 parts, golden cypress 1 ~ 2 part, purple perilla 45 ~ 50 parts, castor-oil plant 2 ~ 3 parts; In the application for a patent for invention " a kind of kill microemulsion of peach brown rot fungus and preparation method thereof " that publication number is " CN101695301A ", described active ingredient is jamaicin and salicylic acid; The application for a patent for invention " a kind of suppress the slow-releasing microcapsule of Monilinia fructicola and the preparation method of composite membrane " that publication number is " CN101574083A " relate to a kind of suppress Monilinia fructicola slow-releasing microcapsule and composite membrane in, its main component is shitosan and jamaicin.
Wei Fengbiao etc. apply for a patent " a kind of Physcion soluble granule and its preparation method and application, publication number CN102440243A ", the invention provides a kind of preparation method of Physcion soluble granule; Explain and clear greatly etc. apply for a patent " a kind of composition pesticide of controlling plant diseases and application; publication number CN1961667A ", the composition pesticide that this invention relates to a kind of controlling plant diseases contains Physcion and Chrysophanol, and the weight ratio that wherein Physcion and Chrysophanol combine is 1: 1 ~ 1: 9; Publication number is in the application for a patent for invention " emodin monomethyl ether for preventing plant fungus disease " of " CN1602692A ", emodin monomethyl ether for preventing plant fungus disease is for preventing and treating the biologic product of fungal diseases of plants, and this biologic product is the secondary metabolites Physcion that plant produces.
Making a general survey of above-mentioned patent, is mostly carry out mixture with jamaicin or Physcion and plant extracts or agricultural chemicals, and patent on phytopathogen of jamaicin and Physcion composite usage and product are had no open report so far.
Summary of the invention
The object of the invention is to overcome above-mentioned the deficiencies in the prior art, there is provided a kind of with the bactericide of botanical components jamaicin and the Physcion synergistic complex that is Fungicidal active substance and application thereof, it adds additive compound with jamaicin and Physcion form for main component, is mainly used in control melon, the agricultural disease such as powdery mildew, gray mold, anthracnose, downy mildew, phytophthora root rot, brown rot, moire blight really, on vegetables and tea tree.
A kind of Botanical synergistic compound bactericide, by weight percentage, the composition of this bactericide comprises: 0.1 ~ 20% jamaicin and 0.1 ~ 20% Physcion.Described jamaicin and Physcion part by weight are 2:1 ~ 1:3.
In above-mentioned bactericide, by weight percentage, also comprise synergist, its synergist accounts for 0 ~ 20%; Described synergist is the mixture of one or more in organosilicon, Butacide, Octacide 264, alkyl carbonate or azone.
In the present invention, the dosage form of Botanical synergistic compound bactericide is environment-friendly type missible oil, microemulsion or aqueous emulsion; Wherein:
Described environment-friendly type missible oil is prepared by following raw materials by weight:
Jamaicin: 0.1 ~ 20%, Physcion: 0.1 ~ 20%, emulsifier: 5 ~ 15%, synergist: 0 ~ 20%, surplus is organic solvent; Its gross weight meets 100%.
Described microemulsion is prepared by following raw materials by weight:
Jamaicin: 0.1 ~ 20%, Physcion: 0.1 ~ 20%, organic solvent: 0 ~ 20%, surfactant: 5 ~ 15%, synergist: 0 ~ 20%, antifreezing agent: 4 ~ 10%, surplus is water; Its gross weight meets 100%.
Described aqua is prepared by following raw materials by weight:
Jamaicin: 0.1 ~ 20%, Physcion: 0.1 ~ 20%, organic solvent: 0 ~ 20%, surfactant: 5 ~ 20%, synergist: 0 ~ 20%, antifreezing agent: 0 ~ 10%, thickener: 0.2 ~ 1%, surplus is water; Its gross weight meets 100%.
Described soluble liquid is prepared by following raw materials by weight:
Jamaicin: 0.1 ~ 20%, Physcion: 0.1 ~ 20%, surfactant: 5 ~ 20%, synergist: 0 ~ 20%, surplus is organic solvent; Its gross weight meets 100%.
In the present invention, described organic solvent is the mixture of one or more in ethyl acetate, cyclohexanone, methyl oleate, ethanol, methyl alcohol, acetone, dimethyl formamide; Described emulsifier is the mixture of one or more in alkyl benzene sulfonate, alkylphenol polyoxyethylene class, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers and similar substance thereof, this Pan's series, tween; Described surfactant is one or more mixture in alkyl benzene sulfonate, alkylphenol polyoxyethylene class, phenethyl phenol polyethenoxy ethers, the addition product of phenethyl phenol polyethenoxy ether polyoxypropylene ethers and similar substance, castor oil and oxirane or aralkyl phenol polyethenoxy ether formaldehyde condensation products; Described antifreezing agent is ethylene glycol or propane diols; Described thickener is the mixture of one or more in polyvinyl alcohol, xanthans, sodium carboxymethylcellulose, Magnesiumaluminumsilicate.
The present invention also provides the application of Botanical synergistic compound bactericide, it may be used for preventing and treating melon, the agricultural disease such as powdery mildew, gray mold, anthracnose, downy mildew, phytophthora root rot, brown rot, moire blight really, on vegetables and tea tree, there is good control efficiency, on organic farm products is produced, have very big application and popularization value, be desirable novel non-pollution bactericide.
Compared with prior art, advantage of the present invention is as follows:
1. jamaicin and Physcion are natural active matter, easily degrade in the environment, and to mammal low toxicities such as people and animals, to pest natural enemy and other beneficial organism safety, and germ not easily develops immunity to drugs to it.
2. adopted formulation is mainly environment-friendly type missible oil, microemulsion, aqua etc., without aromatic hydrocarbon solvents such as dimethylbenzene, greatly reduces the destruction to environment, reduces the murder by poisoning to operator in dispenser process.
3. photostability raising, lasting period are suitable for, and can reduce dispenser frequency and cost accounting, and be conducive to alleviating the pressure to environment further.
4. the economic crops disease such as pair powdery mildew, gray mold, anthracnose, downy mildew, phytophthora root rot, brown rot, moire blight has good control efficiency, can obtain larger economy, society and ecological benefits.
Embodiment
Botanical synergistic compound bactericide of the present invention, with jamaicin and Physcion for active component, adds a certain proportion of solvent and cosolvent and other auxiliary agent, carries out formulation, can obtain environment-friendly type missible oil, microemulsion, aqua and soluble liquid.Wherein, jamaicin consumption by weight percentage 0.1 ~ 20%, Physcion consumption by weight percentage 0.1 ~ 20%, all the other are auxiliary agents.
Test through inventor proves, in Botanical synergistic compound bactericide of the present invention, jamaicin and Physcion part by weight are between 2:1 ~ 1:3 be suitable, dosage form is environment-friendly type missible oil, microemulsion or aqueous emulsion, is mainly used in economic crops diseases such as preventing and treating powdery mildew, gray mold, anthracnose, downy mildew, phytophthora root rot, brown rot, moire blight.
1. environment-friendly type missible oil: jamaicin consumption by weight 0.1 ~ 20%, Physcion consumption by weight 0.1 ~ 20%, all the other are auxiliary agents, and auxiliary agent comprises: emulsifier, organic solvent, synergist.
Emulsifier: 5 ~ 10%, is selected from the mixture of one or more in alkyl benzene sulfonate, alkylphenol polyoxyethylene class, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers and similar substance thereof, Well201, Well205, M30, this Pan's series or tween;
Synergist: 0 ~ 20%, is selected from the mixture of one or more in organosilicon, Butacide, Octacide 264, alkyl carbonate or azone;
Surplus is organic solvent together to 100%: the mixture being selected from one or more in ethyl acetate, acetone or alcohol.
2. microemulsion, content of berberine by weight 0.1 ~ 20%, Physcion 0.1 ~ 20%; All the other are auxiliary agents, and auxiliary agent comprises: surfactant, organic solvent, synergist, antifreezing agent, water.
Surfactant: 5 ~ 30% is one or more mixture in the addition product of alkyl benzene sulfonate, alkylphenol polyoxyethylene class, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers and similar substance, castor oil and oxirane, aralkyl phenol polyethenoxy ether formaldehyde condensation products;
Organic solvent: 0 ~ 20%, to be selected from methyl alcohol, cyclohexanone, dimethyl formamide, dimethyl sulfoxide (DMSO), ethyl acetate, acetone, ethanol the mixture of one or more;
Synergist: 0 ~ 20%, is selected from the mixture of one or more in organosilicon, Butacide, Octacide 264, alkyl carbonate, azone;
Antifreezing agent: 4 ~ 10%, is selected from ethylene glycol, propane diols a kind of;
Surplus use water polishing to 100%.
3. aqua: content of berberine by weight 0.1 ~ 20%, Physcion 0.1 ~ 20%; All the other are auxiliary agents, and auxiliary agent comprises: surfactant, organic solvent, synergist, antifreezing agent and water.
Surfactant: 5 ~ 20%, to be selected from alkyl phosphate class, alkylphenol polyoxyethylene phosphoric acid ester, aralkyl phenol polyethenoxy ether phosphoric acid ester, aliphatic acid polyethenoxy ether class, phenethyl phenol polyethenoxy ethers, the addition product of phenethyl phenol polyethenoxy ether polyoxypropylene ethers and analog, castor oil and oxirane or aralkyl phenol polyethenoxy ether formaldehyde condensation products the mixture of one or more;
Organic solvent: 0 ~ 20%, to be selected from methyl alcohol, cyclohexanone, dimethyl formamide, dimethyl sulfoxide (DMSO), ethyl acetate, acetone or alcohol the mixture of one or more;
Synergist: 0 ~ 20%, is selected from the mixture of one or more in organosilicon, Butacide, Octacide 264, alkyl carbonate or azone;
Antifreezing agent: 0 ~ 10%, is selected from any one of ethylene glycol or propane diols;
Surplus use water polishing to 100%.
4. soluble liquid: content of berberine by weight 0.1 ~ 20%, Physcion 0.1 ~ 20%; All the other are auxiliary agents, and auxiliary agent comprises: surfactant, organic solvent, synergist and organic solvent.
Surfactant: 5 ~ 20%, to be selected from alkyl phosphate class, alkylphenol polyoxyethylene phosphoric acid ester, aralkyl phenol polyethenoxy ether phosphoric acid ester, aliphatic acid polyethenoxy ether class, phenethyl phenol polyethenoxy ethers, the addition product of phenethyl phenol polyethenoxy ether polyoxypropylene ethers and analog, castor oil and oxirane, aralkyl phenol polyethenoxy ether formaldehyde condensation products the mixture of one or more;
Synergist: 0 ~ 20%, is selected from the mixture of one or more in organosilicon, Butacide, Octacide 264, alkyl carbonate, azone;
Organic solvent: the mixture polishing to 100% being selected from methyl alcohol, cyclohexanone, dimethyl formamide, dimethyl sulfoxide (DMSO), ethyl acetate, acetone, ethanol one or more;
The production method of above-mentioned Botanical synergistic compound bactericide environment-friendly type missible oil is: raw material is weighed medicament by formula, adds mixing successively, and stir in a kettle., after quality inspection, finally make finished product.
The production method of above-mentioned Botanical synergistic compound bactericide microemulsion is: weighed by composition of raw materials, add mixing successively, stir transparent in a kettle., after quality inspection, finally make finished product.
The production method of above-mentioned Botanical synergistic compound bactericide aqua is: weighed by formula by raw material, add mixing successively, stir transparent in a kettle., after quality inspection, finally make finished product.
The production method of above-mentioned Botanical synergistic compound bactericide soluble liquid is: weighed by formula by raw material, add mixing successively, stir transparent in a kettle., after quality inspection, finally make finished product.
Embodiment
In order to better understand essence of an invention, describe the technology contents of invention below in detail by embodiment, but the content of invention is not limited to these embodiments.
Embodiment 1:3wt% jamaicin Physcion environment-friendly type missible oil is prepared
At room temperature, count by weight, get 3 parts of jamaicin Physcions (the composite ratio of jamaicin and Physcion is 1:2), said mixture basis adds 6 parts of organosilicons successively, 4 parts of neopelexes and 1 part of OPEO and 1 part of phenethyl phenol polyethenoxy polyethenoxy ether, 100 parts are complemented to ethanol+ethyl acetate+dimethyl formamide (6:3.5:0.5), stir 10 ~ 30 minutes with mixing speed 100 ~ 200 revs/min, 3wt% jamaicin Physcion environment-friendly type missible oil can be obtained.
Embodiment 2:2wt% jamaicin Physcion environment-friendly type missible oil is prepared
At room temperature, count by weight, get 2 parts of jamaicin Physcions (the composite ratio of jamaicin and Physcion is 1:2), said mixture basis adds 3 parts of organosilicons successively, 2 parts of neopelexes and 1 part of OPEO and 1 part of phenethyl phenol polyethenoxy polyethenoxy ether, 100 parts are complemented to ethanol+ethyl acetate+dimethyl formamide (7:2.8:0.2), stir 10 ~ 30 minutes with mixing speed 100 ~ 200 revs/min, 2% jamaicin Physcion environment-friendly type missible oil can be obtained.
Embodiment 3:1.2wt% jamaicin Physcion environment-friendly type missible oil is prepared
At room temperature, count by weight, get 1.2 parts of jamaicin Physcions (the composite ratio of jamaicin and Physcion is 2:1), said mixture basis adds 2 parts of organosilicons successively, 1 part of neopelex and 1 part of OPEO and 1 part of phenethyl phenol polyethenoxy polyethenoxy ether, 100 parts are complemented to ethanol+ethyl acetate+dimethyl formamide (6:3.5:0.5), stir 10 ~ 30 minutes with mixing speed 100 ~ 200 revs/min, 1.2wt% jamaicin Physcion environment-friendly type missible oil can be obtained.
Embodiment 4:1.8wt% jamaicin Physcion aqua is prepared
At room temperature, count by weight, get 1.8 parts of jamaicin Physcions (the composite ratio of jamaicin and Physcion is 1:1), 1 part of organosilicon is added successively, 5 parts of polyoxyethylene nonylphenol ether, thermally coupled distillation columns 3 parts in said mixture, glycerine 4 parts, mix rear solution polishing to 100 part, stir 10 ~ 30 minutes with mixing speed 100 ~ 200 revs/min, 1.8wt% jamaicin Physcion aqua can be obtained.
Embodiment 5:1wt% jamaicin Physcion aqua is prepared
At room temperature, count by weight, get 1 part of jamaicin Physcion (the composite ratio of jamaicin and Physcion is 1:1), in said mixture, add 1 part of organosilicon successively, 3 parts of polyoxyethylene nonylphenol ether, thermally coupled distillation columns 2 parts, glycerine 3 parts, mixes rear solution polishing to 100 part, stirs 10 ~ 30 minutes with mixing speed 100 ~ 200 revs/min,, 1wt% jamaicin Physcion aqua can be obtained.
Embodiment 6:0.6wt% jamaicin Physcion aqua is prepared
At room temperature, count by weight, get 0.6 part of jamaicin Physcion (the composite ratio of jamaicin and Physcion is 1:2), 1 part of organosilicon is added successively, 2 parts of polyoxyethylene nonylphenol ether, thermally coupled distillation columns 1 part in said mixture, glycerine 2 parts, aqueous solution polishing to 100 part, stirs 10 ~ 30 minutes with mixing speed 100 ~ 200 revs/min, can obtain 0.6wt% jamaicin Physcion aqua.
Embodiment 7:3wt% jamaicin Physcion microemulsion is prepared
At room temperature, count by weight, get 3 parts of part jamaicin Physcions (the composite ratio of jamaicin and Physcion is 1:2), then in said mixture, add 2 parts of organosilicons, 6 parts of calcium dodecyl benzene sulfonates, 3 parts of styrenated phenol polyoxyethylene (n30) ethers, 2 parts of ethylene glycol successively, water polishing to 100 part, stir 10 ~ 30 minutes with mixing speed 100 ~ 200 revs/min after mixing, 3wt% jamaicin Physcion microemulsion can be obtained.
Embodiment 8:2wt% jamaicin Physcion microemulsion is prepared
At room temperature, count by weight, get 2 parts of part jamaicin Physcions (the composite ratio of jamaicin and Physcion is 1:2), then in said mixture, add 1 part of organosilicon, 3 parts of calcium dodecyl benzene sulfonates, 2 parts of styrenated phenol polyoxyethylene (n30) ethers, 1 part of ethylene glycol successively, water polishing to 100 part, stir 10 ~ 30 minutes with mixing speed 100 ~ 200 revs/min after mixing, 2wt% jamaicin Physcion microemulsion can be obtained.
Embodiment 9:1.2wt% jamaicin Physcion microemulsion is prepared
At room temperature, get 1.2 parts of jamaicin Physcions (the composite ratio of jamaicin and Physcion is 1:2), then in said mixture, add 1 part of organosilicon, 2 parts of calcium dodecyl benzene sulfonates, 1 part of styrenated phenol polyoxyethylene (n30) ether, 1 part of ethylene glycol successively, water polishing to 100 part, stir 10 ~ 30 minutes with mixing speed 100 ~ 200 revs/min after mixing, 1.2wt% jamaicin Physcion microemulsion can be obtained.
Embodiment 10:1.8wt% jamaicin Physcion soluble liquid is prepared
At room temperature, count by weight, get 1.8 parts of jamaicin Physcions (the composite ratio of jamaicin and Physcion is 1:1), 5 parts of polyoxyethylene nonylphenol ether are added successively in said mixture, thermally coupled distillation columns 3 parts, glycerine 4 parts of organic solvent polishings to 100 part, stir 10 ~ 30 minutes with mixing speed 100 ~ 200 revs/min after mixing, can obtain 1.8wt% jamaicin Physcion soluble liquid.
Embodiment 11:1wt% jamaicin Physcion soluble liquid is prepared
At room temperature, get 1 part of jamaicin Physcion (the composite ratio of jamaicin and Physcion is 1:1), 3 parts of polyoxyethylene nonylphenol ether are added successively in said mixture, thermally coupled distillation columns 2 parts, glycerine 3 parts, with organic solvent polishing to 100 part, stir 10 ~ 30 minutes with mixing speed 100 ~ 200 revs/min after mixing, 2wt% jamaicin Physcion soluble liquid can be obtained.
Embodiment 12:0.6wt% jamaicin Physcion soluble liquid is prepared
At room temperature, count by weight, get 0.6 part of jamaicin Physcion (the composite ratio of jamaicin and Physcion is 1:1), 2 parts of polyoxyethylene nonylphenol ether are added successively in said mixture, thermally coupled distillation columns 1 part, glycerine 2 parts, with organic solvent polishing to 100 part, stir 10 ~ 30 minutes with mixing speed 100 ~ 200 revs/min after mixing, 0.6wt% jamaicin Physcion soluble liquid can be obtained.
The field control effectiveness test of embodiment 13: ten two Plants source synergistic complex bactericide botrytis cinerea
For the field efficacy situation of clear and definite 12 Plants source synergistic complex bactericide, in Yishui County in Shandong Province, the drug effect test of field zone preventing and treating graw mold of tomato is carried out to this medicament.
1 reagent agent
3wt% jamaicin Physcion microemulsion, medicament code name XDW1;
2wt% jamaicin Physcion microemulsion, medicament code name XDW2;
1.2wt% jamaicin Physcion microemulsion, medicament code name XDW3;
1.8wt% jamaicin Physcion aqua, medicament code name XDS1;
1wt% jamaicin Physcion aqua, medicament code name XDS2;
0.6wt% jamaicin Physcion aqua, medicament code name XDS3;
3wt% jamaicin Physcion environment-friendly type missible oil, medicament code name XDR1;
2wt% jamaicin Physcion environment-friendly type missible oil, medicament code name XDR2;
1.2wt% jamaicin Physcion environment-friendly type missible oil, medicament code name XDR3;
1.8wt% jamaicin Physcion soluble liquid, medicament code name XDY1;
1wt% jamaicin Physcion soluble liquid, medicament code name XDY2;
0.6wt% jamaicin Physcion soluble liquid, medicament code name XDY3;
0.5wt% jamaicin aqua, Zhejiang Huajing Bological Development Co., Ltd.;
0.5wt% Physcion aqua, Inner Mongolia Kingbo Biotech Co., Ltd.;
The phonetic mould amine wetting powder of 40wt%, Shaanxi Kang He Li Feng biotechnology pharmaceutcal corporation, Ltd.
2 test article
Syringe (10ml) 3, knapsack sprayer two, drop (300), Enamel jar (1000ml) 1,3,1000ml graduated cylinder, 2,100ml graduated cylinder, pencil 3, minute book 1, and one, camera.
3 for studying thing
Tomato.
4 experimental scheme and method
Small plot experiment is randomized arrangement, and repeat 3 times, plot area is depending on actual conditions.It is 500 that reagent agent is diluted to concentration respectively by the content of active ingredient, 250,125 μ g/ml carry out blade face conventional spray (namely blade face occurring that water membrane is advisable), the phonetic mould amine wetting powder of 40wt% 1000 times of liquid carry out blade face conventional spray for contrasting medicament, and establish clear water to contrast.Investigate according to the classification of blank incidence.Adopt following stage division:
0 grade: anosis;
1 grade: lesion area accounts for less than 5% of one-piece blade area;
3 grades: lesion area accounts for 6% ~ 15% of one-piece blade area;
5 grades: lesion area accounts for 16 ~ 25% of one-piece blade area;
7 grades: lesion area accounts for 26 ~ 50% of one-piece blade area;
9 grades: lesion area accounts for more than 50% of one-piece blade area;
According to survey data, calculate disease index and the control efficiency of each process.
Disease index is pressed formula (1) and is calculated, and result of calculation retains 2 significant digits:
In formula:
X-----disease index;
N
i-----sick number of sheets at different levels;
I-----relative level numerical value;
N-----investigates total number of sheets.
Control efficiency is pressed formula (2) and is calculated:
In formula:
P----control efficiency, unit is percentage (%);
CK----blank disease index;
PT----chemicals treatment disease index;
5 result of the tests and statistics
Adopt conventional spray-on process to jamaicin and Physcion 12 kinds of built agents the field efficacy to graw mold of tomato test, the results are shown in Table 1.
Table 1 12 Plants source bactericide is to the field control effectiveness test result of graw mold of tomato
Note: in " preventive effect " hurdle, marks identical lowercase person and represents in variance analysis (DMRT method) after ordered series of numbers, without significant difference in 5% level, lower same.
As can be seen from the above table, when 12 Plants source medicament active ingredient concentration are 500 μ g/ml, preventive effect, all more than 80%, contrasts the control efficiency of 40% phonetic mould amine wetting powder, 1000 times of liquid without significant difference with medicament.In general, is good to graw mold of tomato control efficiency compared with other medicaments with medicament XDW-1, medicament XDS-2, medicament XDS-3, medicament XDW-2, medicament XDY-3 and medicament XDR-2 in 12 Plants source medicaments.
Embodiment 14: ten two Plants source synergistic complex bactericidal agent for preventing and treating peach brown rot field control effectiveness test
Carried out the drug effect test of field zone of 12 Plants source synergistic complex bactericidal agent for preventing and treating peach brown rotes at Pinggu County, beijing, experimental scheme is with embodiment 13, and contrast medicament is 24wt% RH-7592 suspending agent, and test of pesticide effectiveness result is as table 2:
Table 2 botanical fungicide is to the field control effectiveness test result of peach brown rot
As can be seen from the above table, when 12 Plants source medicament active ingredient concentration are 800 μ g/ml, preventive effect is all more than 75%, and wherein 4 medicine based on herbs contrast the control efficiency of 24% RH-7592,1000 times of liquid without significant difference.In general, be good to the control efficiency of Monilinia fructicola compared with other medicaments with medicament XDR-1, medicament XDR-2, medicament XDW-1, medicament XDY-2 and medicament XDS-2 in 12 Plants source medicaments.
Embodiment 15: ten two Plants source synergistic complex bactericidal agent for preventing and treating apple anthracnose field control effectiveness test
Carried out the drug effect test of field zone of 12 Plants source synergistic complex bactericidal agent for preventing and treating apple anthracnoses in Qixia, Shandong, experimental scheme is with embodiment 13, and contrast medicament is 80% carbendazol wettable powder, 1000 times of liquid, and test of pesticide effectiveness result is as table 3:
Table 3 botanical fungicide is to the field control effectiveness test result of apple anthracnose
As can be seen from the above table, when 12 Plants source medicament active ingredient concentration are 500 μ g/ml, preventive effect, all more than 80%, contrasts the control efficiency of 80% carbendazol wettable powder 1000 times without significant difference with medicament.In general, be good to the control efficiency of apple anthracnose compared with other medicaments with medicament XDR-1, medicament XDW-1, medicament XDY-1 and medicament XDW-2 in 12 Plants source medicaments.
The field experiment result of comprehensive above three tables, 12 Plants source bactericides, in field trial, all show stronger bacteriostatic activity, and some medicament and chemical agent bacteriostasis property are without significant difference; Compared with two kinds of single doses, all show the effect improving preventive effect.Analyze reason, this may be determined by the constituent of active substance of plant complexity, and the essence of effect is just because of result interactional between Multiple components.In addition, the botanical fungicide that the present invention is correlated with is to non-target organism and environmental friendliness, therefore very applicable in the control of economic crops and organic food disease, has very large application and popularization value.
Claims (12)
1. a Botanical synergistic compound bactericide, is characterized in that, by weight percentage, the composition of this bactericide comprises: 0.1 ~ 20% jamaicin and 0.1 ~ 20% Physcion; And described jamaicin and Physcion part by weight are 2:1 ~ 1:3.
2. bactericide according to claim 1, is characterized in that, also comprise synergist in this bactericide, percentage, it accounts for 0 ~ 20%; Described synergist is the mixture of one or more in organosilicon, Butacide, Octacide 264, alkyl carbonate, azone.
3. bactericide according to claim 1 and 2, is characterized in that, the form of this bactericide is environment-friendly type missible oil, microemulsion, aqua or soluble liquid.
4. bactericide according to claim 3, is characterized in that, described environment-friendly type missible oil is prepared by following raw materials by weight:
Jamaicin: 0.1 ~ 20%, Physcion: 0.1 ~ 20%, emulsifier: 5 ~ 10%, synergist: 0 ~ 20%, surplus is organic solvent.
5. bactericide according to claim 3, is characterized in that, described microemulsion is prepared by following raw materials by weight:
Jamaicin: 0.1 ~ 20%, Physcion: 0.1 ~ 20%, organic solvent: 0 ~ 20%, surfactant: 5 ~ 15%, synergist: 0 ~ 20%, antifreezing agent: 4 ~ 10%, surplus is water, and its gross weight meets 100%.
6. bactericide according to claim 3, is characterized in that, described aqua is prepared by following raw materials by weight:
Jamaicin: 0.1 ~ 20%, Physcion: 0.1 ~ 20%, organic solvent: 0 ~ 20%, surfactant: 5 ~ 20%, synergist: 0 ~ 20%, antifreezing agent: 0 ~ 10%, thickener: 0.2 ~ 1%, surplus is water, and its gross weight meets 100%.
7. bactericide according to claim 3, is characterized in that, described soluble liquid is prepared by following raw materials by weight:
Jamaicin: 0.1 ~ 20%, Physcion: 0.1 ~ 20%, surfactant: 5 ~ 20%, synergist: 0 ~ 20%, surplus is organic solvent, and its gross weight meets 100%.
8. bactericide according to claim 4, is characterized in that: described organic solvent is the mixture of one or more in ethyl acetate, cyclohexanone, methyl oleate, ethanol, methyl alcohol, acetone or dimethyl formamide; Described emulsifier is the mixture of one or more in alkyl benzene sulfonate, alkylphenol polyoxyethylene class, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers, Well201, Well205, M30, this Pan's series or tween.
9. according to the arbitrary described bactericide of claim 5-7, it is characterized in that: described surfactant is one or more mixture in the addition product of alkyl benzene sulfonate, alkylphenol polyoxyethylene class, phenethyl phenol polyethenoxy ethers, phenethyl phenol polyethenoxy ether polyoxypropylene ethers, castor oil and oxirane or aralkyl phenol polyethenoxy ether formaldehyde condensation products.
10. the bactericide according to claim 5 or 6, is characterized in that: described antifreezing agent is ethylene glycol or propane diols.
11. bactericide according to claim 6, is characterized in that: described thickener is the mixture of one or more in polyvinyl alcohol, xanthans, sodium carboxymethylcellulose or Magnesiumaluminumsilicate.
The application of powdery mildew, gray mold, anthracnose, downy mildew, phytophthora root rot, brown rot, moire blight agricultural disease prevented and treated by Botanical synergistic compound bactericide described in 12. claims 1 or 2.
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| CN109042776A (en) * | 2018-08-10 | 2018-12-21 | 郭建新 | A kind of nuisanceless environmental protection prevention and treatment apple tree canker preparation |
| CN109090126B (en) * | 2018-08-21 | 2021-06-08 | 北京联合大学 | Plant source compound synergistic bactericide and application thereof |
| CN111387200A (en) * | 2020-04-16 | 2020-07-10 | 中国科学院上海生命科学研究院湖州现代农业生物技术产业创新中心 | Biopesticide composition for preventing and treating anthracnose of fresh-eating jujubes in south and using method thereof |
| CN112674125A (en) * | 2020-12-31 | 2021-04-20 | 天津大学 | Plant source green pesticide compound agent and preparation method thereof |
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| CN102894002A (en) * | 2012-11-05 | 2013-01-30 | 山东省烟台市农业科学研究院 | Compound bactericide containing berberine and polyoxins and application thereof |
| CN103444729A (en) * | 2013-09-05 | 2013-12-18 | 肇庆长龙生物科技有限公司 | Bactericidal pharmaceutical composition and applications thereof |
| CN103548849A (en) * | 2013-10-12 | 2014-02-05 | 山东省烟台市农业科学研究院 | Synergistic compound biological bactericide and application thereof |
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| CN102894002A (en) * | 2012-11-05 | 2013-01-30 | 山东省烟台市农业科学研究院 | Compound bactericide containing berberine and polyoxins and application thereof |
| CN103444729A (en) * | 2013-09-05 | 2013-12-18 | 肇庆长龙生物科技有限公司 | Bactericidal pharmaceutical composition and applications thereof |
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