CN111387200A - Biopesticide composition for preventing and treating anthracnose of fresh-eating jujubes in south and using method thereof - Google Patents
Biopesticide composition for preventing and treating anthracnose of fresh-eating jujubes in south and using method thereof Download PDFInfo
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- CN111387200A CN111387200A CN202010298027.9A CN202010298027A CN111387200A CN 111387200 A CN111387200 A CN 111387200A CN 202010298027 A CN202010298027 A CN 202010298027A CN 111387200 A CN111387200 A CN 111387200A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- Pest Control & Pesticides (AREA)
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Abstract
The invention discloses a biopesticide composition for preventing and treating anthracnose of southern fresh-eating jujubes and a use mode thereof. The composition disclosed by the invention belongs to the field of biopesticides, can effectively prevent and control anthracnose of fresh-eating jujubes in south China, and is environment-friendly. The composition disclosed by the invention is used for preventing and treating anthracnose of southern fresh-eating jujubes, has an obvious effect, and can effectively improve the appearance of fruits so as to increase the quantity of commercial fruits.
Description
Technical Field
The invention belongs to the field of plant protection, and particularly relates to a biopesticide composition for preventing and treating anthracnose of southern fresh-eating jujubes and a use method thereof.
Background
The jujube is one of the oldest fruit trees in China and five famous fruits in ancient times in China, is also the first main fruit and the seventh fruit tree in China at present, and has been proved to have at least 5000 years of cultivation history according to historical materials. At present, the jujube production area can be divided into a northern jujube area and a southern jujube area. The southern jujube region is a wide region with the average annual temperature above 15 ℃ and annual precipitation of more than 700mm, and mainly focuses on 5 provinces of Anhui, Zhejiang, Hunan, Hubei and Jiangsu. The south fresh-eating jujube can be sold in the market with the northern jujube in a wrong season, so that the south fresh-eating jujube has a good market, becomes a development hotspot of the south fruits in China, also becomes an important economic forest tree species for the rural areas in the south and the transformation of barren mountains, and is favored by people.
However, most of the cultivation management of fresh-eating jujubes in south is extensive, some production areas are not even managed, the jujubes are lack of fertilizer and water, the diseases and the pests are rampant, the tree vigor is weak, the tree bodies are incomplete, and the yield is very low. Anthracnose is one of main diseases of southern fresh-eating jujubes, after the jujube hangers, the jujube heads and the jujube strands are infected, the early symptoms are not obvious and exist in a latent state, and germs live through the winter on the residual jujube hangers, the jujube heads and the jujube strands by using mycelium and become the initial infection source of the next year together with diseased berries. The southern fresh-eating jujube anthracnose is serious generally in the middle of 7 to 8 months, the fruit is infected and is changed into faint yellow initially, then water stain-shaped spots appear, and meanwhile, irregular tawny spots are accompanied, when the disease is serious, the disease spots are reddish brown and cause early fruit fall, the quantity of commercial fruits is seriously influenced, and great economic loss is caused to farmers.
At present, various control agents for jujube anthracnose exist, for example, a jujube anthracnose vaccine disclosed in 2017, 10 and 17 months, which is published by the patent publication No. CN107251907, comprises penicillin, carbendazim, prochloraz, imazalil aqueous emulsion, indoleacetic acid, a surfactant, sulfur, a copper sulfate solution, an aqueous solution and a boric acid solution. However, the components such as antibiotics are used, so that the drug resistance is easy to generate, and the copper sulfate is toxic and does not meet the requirement of environmental protection. For another example, a green pesticide for preventing and treating pomegranate anthracnose, disclosed in 2018, 12 and 28 months, is disclosed as CN109090161A, and is prepared from the following substances in parts by weight: 6-8 parts of hot pepper, 9-12 parts of valeriana jatamansi jones, 5-7 parts of spina date seeds, 3-6 parts of betel nuts, 4-8 parts of Chinese torreya seeds, 7-10 parts of folium isatidis, 2-5 parts of Japanese ardisia herb, 4-7 parts of fructus liquidambaris and 6-9 parts of cortex toonae sinensis. Although disease resistance is not easy to generate, and the composition is green and safe, the stability is difficult to control due to more components, the drug effect control difficulty is high, and the drug effect duration is short.
In addition, for example, the Chinese patent with patent publication No. CN103981137B discloses a strain for antagonizing pathogenic bacteria of jujube fruit shrink disease and its application, and specifically discloses various applications of the disclosed antagonistic bacterial strain Bacillus subtilis STO-45 in preventing and treating jujube fruit shrink disease, jujube anthracnose disease, and related tree diseases caused by Humicola melanosporum, Humicola apple, Ralstonia persica, Fusarium oxysporum and Fusarium solani. However, the prevention and treatment effect is only in the test stage, and the effect is not ideal due to the influence of various environmental factors in actual use.
Disclosure of Invention
The invention provides a biopesticide composition for preventing and treating southern fresh-eating jujube anthracnose, which can effectively prevent and treat southern fresh-eating jujube anthracnose, combines short-term protection and long-term protection for fresh-eating jujube, and improves the yield of southern fresh-eating jujube commodity fruits.
In order to achieve the purpose, the invention provides a biopesticide composition for preventing and treating southern fresh-eating jujube anthracnose, and the active ingredients of the composition comprise bacillus subtilis, matrine and berberine.
The matrine and the berberine in the biological pesticide composition can effectively prevent the occurrence of southern fresh-eating jujube anthracnose and can also quickly control the spread of the southern fresh-eating jujube anthracnose; the bacillus subtilis in the composition can be rapidly proliferated, so that long-acting protection is realized in the picking period of fresh jujube trees in south China; the combination of short-acting effect and long-acting effect is more beneficial to preventing and controlling anthracnose of fresh-eating jujubes in south China.
The bacillus subtilis is preserved in China general microbiological culture collection center with the preservation date of 2011, 12 and 26 days, the preservation number of CGMCC No.5652 and the preservation address of Beijing City. The biological name of the Bacillus subtilis isBacillus subtilis。
Preferably, the preparation method of the bacillus subtilis comprises the steps of ① activating strains by using a L B culture medium (yeast extract is 5 g/L, peptone is 10 g/L, sodium chloride is 10 g/L, and pH is 7.5), ② inoculating the cultured liquid strains into a liquid fermentation culture medium according to the inoculation amount of 3%, culturing for 36-48 hours at ③ 30 ℃ and stopping fermentation when the sampling microscopic spore rate reaches more than 95%, adsorbing by using 200-mesh diatomite at ④, drying at 45 ℃ and preparing 200/g bacterial powder.
Preferably, the liquid culture medium formula is as follows: glucose in weight percent: 0.5% -1%, corn steep liquor: 1% -5%, peptone: 1% -3%, MgCl 2: 0.05-0.08%, MnSO 4: 0.05% -0.08%, K2HPO 4: 0.1-0.6 percent, and the balance of water.
The matrine and berberine are commercial raw medicines or preparations.
Preferably, the biopesticide composition for preventing and treating anthracnose of southern fresh-eating jujubes comprises 5-45% of bacillus subtilis powder (200 hundred million/g), 0.1-10% of matrine, 0.1-10% of berberine and the balance of water.
Preferably, the biopesticide composition for preventing and treating anthracnose of southern fresh-eating jujubes further comprises a stabilizer CaCO3、K2HPO4、K3PO4Sodium carboxymethylcellulose and CaCl2And NaCl. One or more of the stabilizers are added, which is beneficial to stabilizing the drug effect of the biological pesticide composition.
Preferably, the biopesticide composition for preventing and treating anthracnose of southern fresh-eating jujubes further comprises one or more of protective agents of vitamin C, carboxymethyl cellulose, dextrin and corn starch.
The application method of the biological pesticide composition comprises the steps of respectively applying the biological pesticide composition once every 20-25 days in the fruit setting period from late 6 months to middle 7 months; the medicine is used at least once again in the fruit expansion period of 8-9 months.
The medicine is sprayed and diluted with water before use.
Compared with the prior art, the invention has the following beneficial effects:
(1) the pesticide composition for preventing and treating southern fresh-eating jujubes belongs to the field of biological pesticides, is environment-friendly, and is more beneficial to improving the safety of southern fresh-eating jujubes.
(2) The composition has remarkable effect on preventing and treating anthracnose of fresh-eating jujubes in south, and further can improve the appearance of fruits and increase the quantity of commercial fruits.
(3) In the pesticide composition, the matrine and the berberine can effectively prevent the occurrence of southern fresh-eating jujube anthracnose and can also quickly control the spread of the southern fresh-eating jujube anthracnose; the bacillus subtilis in the composition can be rapidly proliferated, so that long-acting protection is realized in the picking period of fresh jujube trees in south China; the combination of short-acting effect and long-acting effect is more beneficial to preventing and controlling anthracnose of fresh-eating jujubes in south China.
Detailed Description
The embodiments of the present invention will be described in detail with reference to specific examples, but the scope of the invention is not limited by the specific embodiments.
Example 1
The composition comprises 20% of bacillus subtilis powder (200 hundred million/g), 2% of matrine, 5% of berberine, 1% of sodium carboxymethylcellulose, 3% of corn starch, and the balance of water, wherein the rest is diluted by 500 times of water before use. The pesticide effect test scheme is implemented in 1 mu area of the fresh-eating Chinese date in south, and the fresh-eating Chinese date is sprayed for 1 time at the bottom of 6 months, in the middle of 7 months and in the last ten days of 8 months respectively.
The preparation method of the bacillus subtilis comprises the steps of ① activating strains by using a L B culture medium (yeast extract is 5 g/L, peptone is 10 g/L, sodium chloride is 10 g/L and pH is 7.5), ② inoculating the cultured liquid strains into a liquid fermentation culture medium according to the inoculation amount of 3%, culturing for 36-48 hours at ③ 30 ℃, stopping fermentation when the sampling microscopic spore rate reaches more than 95%, absorbing ④ by using 200-mesh diatomite, and drying at 45 ℃ to prepare 200 hundred million/g bacterial powder, wherein the formula of the liquid culture medium comprises 1% of glucose, 5% of corn steep liquor, 3% of peptone, 0.05% of MgCl2, 0.05% of MnSO4, 0.1% of K2HPO4 and the balance of water in percentage by weight.
Example 2
The composition comprises 30% of bacillus subtilis powder (200 hundred million/g), 0.5% of matrine, 3% of berberine, 0.5% of NaCl, 0.5% of CaCO3 and 5% of dextrin, and the balance of water, and is diluted by 300 times before use. The pesticide effect test scheme is implemented in 1 mu area of the fresh-eating Chinese date in south, and the fresh-eating Chinese date is sprayed for 1 time at the bottom of 6 months, in the middle of 7 months and in the last 9 months respectively. The preparation method of Bacillus subtilis is as in example 1.
Example 3
The composition comprises 40% of bacillus subtilis powder (200 hundred million/g), 1% of matrine, 1% of berberine, 2% of sodium carboxymethylcellulose, 3% of vitamin C, and the balance of water, wherein the rest is diluted by 500 times of water before use. The pesticide effect test scheme is implemented in 1 mu area of the fresh-eating Chinese date in south, and the fresh-eating Chinese date is sprayed for 1 time at the bottom of 6 months, in the middle of 7 months and in the last 9 months respectively. The preparation method of Bacillus subtilis is as in example 1.
Example 4
The difference from example 3 is that the composition does not contain stabilizers and protective agents. The composition only comprises 40% of bacillus subtilis powder (200 hundred million/g), 1% of matrine, 1% of berberine and the balance of water.
Comparative example 1
The difference from example 3 is that the composition comprises 40% of Bacillus subtilis powder (200 hundred million/g), 2% of sodium carboxymethylcellulose, 3% of vitamin C, and the balance of water, and the composition does not contain matrine and berberine.
Comparative example 2
The difference from example 3 is that the composition comprises 1% matrine, 2% sodium carboxymethyl cellulose, 3% vitamin C, the remainder being made up with water. The composition does not contain bacillus subtilis powder and berberine.
Comparative example 3
The difference from example 3 is that the composition comprises 1% berberine, 2% sodium carboxymethylcellulose, 3% vitamin C, the remainder being made up with water. The composition does not contain bacillus subtilis powder and matrine.
Comparative example 4
The difference from example 3 is that the composition comprises 1% matrine, 1% berberine, 2% sodium carboxymethylcellulose, 3% vitamin C, the remainder being made up with water. The composition does not contain bacillus subtilis powder and matrine.
Description of the investigation method: in the peak picking period, 5 trees are selected from east, south, west, north and middle of the medicine application area and the control area respectively, 100 fruits are picked at random, and the prevention and treatment effect is counted according to the disease occurrence condition. And after the harvesting period is finished, counting the commodity fruit increase rate according to the weight of the commodity fruits in each area.
Disease fruit rate (%) = (total number of disease fruits/total number of survey fruits) × 100%
Control effect (%) = ((control area fruit disease rate-application area fruit disease rate)/control area fruit disease rate) × 100%
Commercial fruit increase (%) = (weight of commercial fruit in drug area-weight of commercial fruit in control area)/weight of commercial fruit in control area) × 100%
The data show that the biopesticide composition can effectively prevent and control anthracnose of fresh-eating jujubes in south China and obviously improve the quantity of commercial fruits.
Claims (8)
1. A biopesticide composition for preventing and treating anthracnose of southern fresh-eating jujubes is characterized in that effective components of the composition comprise bacillus subtilis, matrine and berberine.
2. The biopesticide composition for controlling southern fresh-eating jujube anthracnose according to claim 1, comprising 5-45% of bacillus subtilis powder (200 hundred million/g), 0.1-10% of matrine, 0.1-10% of berberine, and the balance of water.
3. The biopesticide composition for controlling anthracnose of southern fresh-eating jujubes according to claim 2, wherein the bacillus subtilis is preserved in China general microbiological culture collection center with a preservation date of 2011, 12 months and 26 days and a preservation number of CGMCC No.5652, and the bacillus subtilis is preserved in China general microbiological culture collection center with a preservation number of CGMCC No.5652Biological nomenclature of BacillusBacillus subtilis。
4. The biopesticide composition for controlling southern fresh-eating jujube anthracnose according to claim 1, further comprising 1-10% of a stabilizer, wherein the stabilizer is CaCO3、K2HPO4、K3PO4, sodium carboxymethylcellulose, CaCl2And NaCl.
5. The biopesticide composition for controlling southern fresh-eating jujube anthracnose according to claim 4, further comprising 1-10% of a protective agent, wherein the protective agent is one or more of vitamin C, carboxymethyl cellulose, dextrin, and corn starch.
6. The biopesticide composition for controlling anthracnose of southern fresh-eating jujubes according to claim 5, wherein the composition comprises 5-45% of bacillus subtilis powder (200 hundred million/g), 0.1-10% of matrine, 0.1-10% of berberine, 1-10% of stabilizer, 1-10% of protective agent, and the balance of water.
7. The biopesticide composition according to any one of claims 1 to 6, used in a manner that it is applied once every 20 to 25 days in the fruit setting period from late 6 to mid 7 months; the medicine is used at least once again in the fruit expansion period of 8-9 months.
8. Use of the biopesticide composition according to claim 7, characterized in that spraying is employed.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202010298027.9A CN111387200A (en) | 2020-04-16 | 2020-04-16 | Biopesticide composition for preventing and treating anthracnose of fresh-eating jujubes in south and using method thereof |
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| CN202010298027.9A CN111387200A (en) | 2020-04-16 | 2020-04-16 | Biopesticide composition for preventing and treating anthracnose of fresh-eating jujubes in south and using method thereof |
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| CN105684688A (en) * | 2016-01-18 | 2016-06-22 | 吕文玲 | Jujube tree plantation method |
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| CN109479688A (en) * | 2018-10-25 | 2019-03-19 | 北京市植物保护站 | Potting efficient hot pepper organises implantation methods |
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2020
- 2020-04-16 CN CN202010298027.9A patent/CN111387200A/en active Pending
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|---|---|---|---|---|
| CN103981137A (en) * | 2014-05-23 | 2014-08-13 | 北京林业大学 | Bacterial strain antagonistic to pathogenic bacteria of jujube fruit shrink disease and application of bacterial strain |
| CN104488923A (en) * | 2015-01-09 | 2015-04-08 | 馥稷生物科技发展（上海）有限公司 | Plant source synergistic compound bactericide and application thereof |
| CN105684688A (en) * | 2016-01-18 | 2016-06-22 | 吕文玲 | Jujube tree plantation method |
| CN106719838A (en) * | 2016-11-22 | 2017-05-31 | 恒大园林集团有限公司 | A kind of complex ecological agricultural chemicals and preparation method thereof |
| CN109479688A (en) * | 2018-10-25 | 2019-03-19 | 北京市植物保护站 | Potting efficient hot pepper organises implantation methods |
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