CN109924201A - A kind of composite bactericide of quinones - Google Patents
A kind of composite bactericide of quinones Download PDFInfo
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- CN109924201A CN109924201A CN201910248233.6A CN201910248233A CN109924201A CN 109924201 A CN109924201 A CN 109924201A CN 201910248233 A CN201910248233 A CN 201910248233A CN 109924201 A CN109924201 A CN 109924201A
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Abstract
The present invention provides a kind of composite bactericides containing quinones, its active constituent is hypocrellin A, hypocrelline B and cystolycin A, and wherein the mass ratio of hypocrellin A, hypocrelline B and cystolycin A is (9-14): (1.5-3): (3-9).Composite bactericide containing quinones of the invention, can play good bactericidal effect, and wide sterilization spectrum solves the problems, such as that existing fungicide resistance is serious；And preferable pesticide efficacy can be obtained under the dosage of very little, the residual in crop is few, not can cause environmental pollution；It also added the extract of metal ion and medicinal fungi, it can play the role of efficacy-enhancing ingredient in composite bactericide, it can promote absorption and conduction of the crop to nutritional ingredient in pesticide activity component and soil, improve the disease resistance of crop, enhance drug effect, improve crop constitution, enhance crop metabolism, improves disease resisting effect.
Description
Technical field
The invention belongs to field of pesticides, in particular to a kind of composite bactericide of quinones.
Background technique
With the continuous development of pesticide industry, low toxicity, efficient, less-persistent pesticide increasingly by social extensive concern, are walked
Environmental type Agrochemicals road becomes the main path of research and development novel pesticide, but traditional pesticide, i.e., chemical pesticide is one
Determine also using in degree, however the disadvantages of the generally existing toxicity height of traditional pesticide, drug resistance, how to develop less toxic, efficient
Pesticide becomes the project of researcher's concern.
Summary of the invention
The purpose of the invention is to provide a kind of optimum formula for preventing and treating phytopathogen naphtoquinone compounds, it is enable to be used
In the preparation of fungicide and the prevention and treatment of phytopathogen；It is yet another object of the invention to provide a kind of answering containing quinones
Preferable pesticide efficacy can be obtained under the dosage of very little with fungicide, and the residual in crop is few, it will not be to ring
The method that border pollutes.
For get rid of traditionally treat pathogen pesticide dosage it is big, the status of effect difference, need to open up dosage it is few, to people and animals
Safety, the new pesticide of non agricultural chemical residuum compare the pathogen with harmfulness in order to prevent and treat in Chinese herbal medicine and crops.Benefit
With the characteristic of naphtoquinone compounds, by the method for optimization, the present invention provides a kind of prevention and treatment phytopathogen naphtoquinone compounds
Optimum formula makes it can be used for the preparation of fungicide and the prevention and treatment of phytopathogen.
Technical solution: a kind of composite bactericide containing quinones, active constituent is bamboo in the composite bactericide
Red fungus beetle element, hypocrelline B and cystolycin A, the wherein quality of hypocrellin A, hypocrelline B and cystolycin A
Than for (9-14): (1.5-3): (3-9).
As an improvement, being also added with synergist in composite bactericide, synergist includes metallic ions Ca2+And Mg2+。
As an improvement, metallic ions Ca2+Concentration is 0.045-0.055mol/L, metal ions M g2+Concentration is 0.035-
0.045mol/L。
As an improvement, synergist also adds the extract including medicinal fungi tabasheer ethyl acetate extract in composite bactericide.
As an improvement, the concentration of the extract of addition medicinal fungi tabasheer ethyl acetate extract is 0.06-0.14g/L.
As an improvement, active constituent is hypocrellin A, hypocrelline B and cystolycin A in the composite bactericide
Mass ratio be (9-14): (1.5-3): (3-9) further includes metallic ions Ca2+Concentration is 0.045-0.055mol/L, metal from
Sub- Mg2+Concentration is 0.035-0.045mol/L, and the concentration of the extract of medicinal fungi tabasheer ethyl acetate extract is 0.06-
0.14g/L。
The utility model has the advantages that the present invention is to play the comprehensive benefit containing quinones, explore a kind of containing quinones
The method and its application of composite bactericide be a significant research work.This composite bactericide can play good sterilization
Effect, wide sterilization spectrum solve the problems, such as that existing fungicide resistance is serious.
The present invention is compared with the traditional method, dosage of the composite bactericide of the invention containing naphtoquinone compounds in very little
It is lower to obtain preferable pesticide efficacy, and the residual in crop is few, not can cause environmental pollution, is a kind of novel
Pesticide；It is added to plant extracts in the optimization formula of composite bactericide of the present invention, which can rise in composite bactericide
To the effect of efficacy-enhancing ingredient, absorption and conduction of the crop to nutritional ingredient in pesticide activity component and soil can be promoted, improve crop
Disease resistance, enhance drug effect, improve crop constitution, enhance crop metabolism, improve disease resisting effect.
The above description is only an overview of the technical scheme of the present invention, in order to better understand the technical means of the present invention,
And can be implemented in accordance with the contents of the specification, with presently preferred embodiments of the present invention and cooperation, detailed description is as follows below.
Specific embodiment
With reference to embodiment, the embodiment of the present invention is furthur described in detail.Following embodiment is used for
Illustrate the present invention, but is not intended to limit the scope of the invention.
Naphtoquinone compounds are the derivative of 4,9- dihydroxy -3,10- quinone, are certain from Ascomycetes and Fungi Imperfecti
A kind of novel pigment of separation is extracted in fungi, while being also a kind of substance with photosensitive activity.Quinone provided by the invention
Compound mainly includes hypocrellin and Elsinochrome element, and hypocrellin has anti-inflammatory, analgesia, antibacterial, photosensitive killing tumour thin
Born of the same parents mitigate the effects of toxic side effect and inhibition granulation tissue hyperplasia.Elsinochrome element is in addition to that can be used to treat some skins
It is sick outer, also there is the activity for killing inhibition of HIV, anti-herpes simplex virus and Sindbis virus.
Embodiment 1
Study influence of the mass ratio of various quinones to composite bactericide bacteriostatic activity
1. experimental material and method
1.1 material source
Representative pathogenic bacteria Fusarium solani (Fusarium solani) in root rot, hay bacillus (Bacillus
It subtilis), is the preservation of bio-pharmaceuticals teaching and research room, Binzhou College.PDA solid medium, PD fluid nutrient medium, ordinary broth
Fluid nutrient medium and ordinary broth solid medium etc. conventionally configure, and then sterilize.
The preparation of 1.2 scraps of paper
Filter paper (8mm) is dipped in the dimethyl sulphoxide solution containing different quinones and is protected in the dark in a moment
It deposits, it is spare.
The active measurement of 1.3 composite bactericides
By sterile working regulation, each 100 μ L of tested bacteria suspension is drawn respectively, is added on different plating mediums and is coated with
Uniformly.By preparatory sterilizing, drying and it is soaked with the filter paper of quinones, is flattened on containing on bacterium culture medium.Processing group is at 26 DEG C
Cultivated in 35 DEG C of incubator 48h or for 24 hours after, with whether the generation of inhibition zone and size indicates that composite bactericide is active
Whether there is or not and its height.The bigger expression composite bactericide activity of difference of inhibition zone and scraps of paper diameter is better.
2. experimental result
Influence of the mass ratio of 2.1 various naphtoquinone compounds to bacteriostatic activity
Hypocrellin A, hypocrelline B and Elsinochrome element all have certain fungistatic effect, how their suppression
Bacterium effect obtains maximum performance, and the mass ratio of these three quinones is optimized in the present invention, with fungistatic effect
Size as optimizing index, find on broth medium, the 100 μ L Fusarium solani suspensions for having cultivated 36h be added,
When the mass ratio of hypocrellin A, hypocrelline B and Elsinochrome element is (9-14): (1.5-3): when (3-9), in incubator
Cultivation temperature is 26 DEG C, incubation time 48h, has preferable inhibitory effect.
Embodiment 2
The metal ion of composite bactericide bacteriostatic activity correlative factor and the optimization experimental result of concentration
1. experimental material and method
1.1 material source
Representative pathogenic bacteria Fusarium solani (Fusarium solani) in root rot, hay bacillus (Bacillus
), it subtilis is the preservation of bio-pharmaceuticals teaching and research room, Binzhou College.
PDA solid medium, PD fluid nutrient medium, ordinary broth fluid nutrient medium and ordinary broth solid medium etc. are equal
It conventionally configures, then sterilizes.
The preparation of 1.2 scraps of paper
Filter paper (8mm) is dipped in the dimethyl sulphoxide solution containing different quinones and is protected in the dark in a moment
It deposits, it is spare.
The active measurement of 1.3 composite bactericides
By sterile working regulation, each 100 μ L of tested bacteria suspension is drawn respectively, is added on different plating mediums and is coated with
Uniformly.By preparatory sterilizing, drying and it is soaked with the filter paper of quinones, is flattened on containing on bacterium culture medium.Processing group 26 or
In 35 DEG C of incubator culture 48 or for 24 hours after, with whether the generation of inhibition zone and size has to indicate that composite bactericide is active
Nothing and its height.The bigger expression composite bactericide activity of difference of inhibition zone and scraps of paper diameter is better.
1.4 composite bactericides measure the antimicrobial spectrum of plant pathogenic fungi
Using mycelial growth rate method measurement composite bactericide to the inhibiting effect of test plant pathogen.Draw 50 μ g/
ML composite bactericide dimethyl sulfoxide solution is added in PDA culture medium, is uniformly mixed, and inverted plate enters in the culture dish that diameter is 9cm.
Control is the PDA culture medium of the dimethyl sulfoxide containing equivalent, and blank is not add any reagent PDA culture medium.Every processing sets 3 weights
It is multiple, the pathogen bacteria cake that eugonic diameter is 4mm is accessed into above-mentioned each culture medium.The culture medium of bacterium will be connected with fresh-keeping
Film sealing, is cultivated 2 days under illumination box under the conditions of being placed in 26 DEG C.All processing culture dishes are taken out and measure respective bacterium colony
Diameter, each bacterium colony measure diameter using crossing method.
Mycelial growth inhibition rate is calculated with following equation:
2. experimental result
Influence of the 2.1 each metal ion species to bacteriostatic activity
Hypocrellin A, hypocrelline B and Elsinochrome element all have certain fungistatic effect, how their suppression
Bacterium effect obtains maximum performance, the present invention to synergist is added in quinones, wherein synergist selection metal from
Son is studied, using the size of fungistatic effect as optimizing index.When concentration of metal ions is 0.05mol/L, K is had detected+, Na+Ca2+, Mg2+And Al3+To the inhibiting rate of Fusarium solani, monovalent metallic ion K is found+And Na+Quinones is pressed down
The activity influence of Fusarium solani processed is little；It was found that the 100 μ L Fusarium solanis for having cultivated 36h are added in PDA culture medium,
As addition bivalent metal ion Ca in quinones2+And Mg2+There is certain promotion to the bacteriostatic activity of quinones
Effect, inhibiting rate reach 94% and 93%, Al3+The activity influence having to the bacteriostatic activity of quinones is also little.
Influence of each metal ion species of table 1 to Fusarium solani
| Metal ion | K+ | Na+ | Ca2+ | Mg2+ | Al3+ |
| Inhibiting rate | 67% | 78% | 94% | 93% | 84% |
Influence of 2.2 concentration of metal ions to bacteriostatic activity
Hypocrellin A, hypocrelline B and Elsinochrome element all have certain fungistatic effect, and divalent is added wherein
Metallic ions Ca2+And Mg2+Can reinforce fungistatic effect, how their fungistatic effect be obtained maximum performance, the present invention to
Metallic ions Ca is added in quinones2+And Mg2+Concentration studied, using the size of fungistatic effect as optimization
Index is found in common PDA culture medium, and the 100 μ L Fusarium solanis for having cultivated 36h are added, and finds Ca2+Concentration is 0.045-
0.055mol/L, metal ions M g2+Concentration is 0.035-0.045mol/L, and the inhibitory effect of quinones is obvious, this is
Since the stability of cell membrane declines, so that indicator bacteria is more sensitive for the bacteriostasis of quinones.Simultaneously
It was found that working as metallic ions Ca2+And Mg2+Concentration be 0.05mol/L Ca2+, 0.04mol/L Mg2+When, it is put into 26 DEG C of culture
48h is cultivated in case, the inhibitory effect of quinones is best.As ion Ca2+And Mg2+Concentration is 0.05mol/L,
When 0.04mol/L, the survival rate of Fusarium solani is minimum, is down to 5% or less.
2 difference Ca of table2+Influence of the concentration to Fusarium solani
| Ca2+Concentration | The inhibiting rate of Fusarium solani |
| 0.035 | 80% |
| 0.04 | 89% |
| 0.045 | 91% |
| 0.05 | 94% |
| 0.055 | 93% |
| 0.060 | 88% |
| 0.065 | 77% |
3 difference Mg of table2+Influence of the concentration to Fusarium solani
| Mg2+Concentration | The inhibiting rate of Fusarium solani |
| 0.025 | 80% |
| 0.03 | 85% |
| 0.035 | 91% |
| 0.04 | 93% |
| 0.045 | 90% |
| 0.050 | 85% |
| 0.055 | 77% |
Embodiment 3
Field experiment
Composite bactericide containing naphtoquinone compounds, including hypocrellin A, hypocrelline B and Elsinochrome element
Mass ratio be 9:1.5:3,0.055mol/L Ca2+, 0.035mol/L Mg2+, 0.06g/L bamboo parasitic fungus ethyl acetate extract
Extract.Application method is that composite bactericide is mixed evenly to rear dilution soluble in water, the effective ingredient of composite bactericide
Content is 3%, and fungicide is acted on to common wheatland by the way of Direct spraying, can be with various in effectively preventing wheatland
Phytopathogen, sterilizing rate are controlled 98% or more.
Embodiment 4
Field experiment
Composite bactericide containing naphtoquinone compounds, including hypocrellin A, hypocrelline B and Elsinochrome element
Mass ratio be 11:2.3:8.5,0.049mol/LCa2+, 0.042mol/LMg2+, 0.11g/L bamboo parasitic fungus ethyl acetate extract
Extract.Application method is that composite bactericide is mixed evenly to rear dilution soluble in water, the effective ingredient of composite bactericide
Content is 2.8%, and fungicide is acted on to common wheatland by the way of Direct spraying, can be with each in effectively preventing wheatland
Kind phytopathogen, sterilizing rate are controlled 97% or more.
Embodiment 5
Field experiment
Composite bactericide containing naphtoquinone compounds, including hypocrellin A, hypocrelline B and Elsinochrome element
Mass ratio be 14:3:9,0.045mol/LCa2+, 0.045mol/L Mg2+, 0.14g/L bamboo parasitic fungus ethyl acetate extract mentions
Take object.Application method is that composite bactericide is mixed evenly to rear dilution soluble in water, and the effective ingredient of composite bactericide contains
Amount is 3.5%, and fungicide is acted on to common wheatland by the way of Direct spraying, can be with various in effectively preventing wheatland
Phytopathogen, sterilizing rate are controlled 96% or more.
In conclusion the naphtoquinone compounds of the best bacteriostatic activity and addition of the composite bactericide containing naphtoquinone compounds
Type；The mass ratio of naphtoquinone compounds；The type of the metal ion of addition；Add the concentration of metal ion；The tabasheer of addition mentions
Take the type of object；The concentration of addition tabasheer extract has close relationship.Properly matching containing naphtoquinone compounds composite bactericide
Side: the mass ratio of hypocrellin A, hypocrelline B and Elsinochrome element is (9-14): (1.5-3): (3-9), 0.045-
0.055mol/LCa2+, 0.035-0.045mol/LMg2+, the extract of 0.06-0.14g/L bamboo parasitic fungus ethyl acetate extract.This
The fungistatic effect of the composite bactericide of sample composition is significant, excellent.
The embodiments described above only express several embodiments of the present invention, and the description thereof is more specific and detailed, but simultaneously
It cannot therefore be construed as limiting the scope of the patent.It should be pointed out that coming for those of ordinary skill in the art
It says, without departing from the inventive concept of the premise, various modifications and improvements can be made, these belong to protection of the invention
Range.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Claims (6)
1. a kind of composite bactericide containing quinones, it is characterised in that: active constituent is bamboo in the composite bactericide
Red fungus beetle element, hypocrelline B and cystolycin A, the wherein quality of hypocrellin A, hypocrelline B and cystolycin A
Than for (9-14): (1.5-3): (3-9).
2. composite bactericide according to claim 1, it is characterised in that: be also added with synergist, packet in composite bactericide
Include metallic ions Ca2+And Mg2+。
3. composite bactericide according to claim 2, it is characterised in that: metallic ions Ca2+Concentration is 0.045-
0.055mol/L, metal ions M g2+Concentration is 0.035-0.045mol/L.
4. composite bactericide according to claim 2, it is characterised in that: synergist further includes medicinal true in composite bactericide
The extract of bacterium tabasheer ethyl acetate extract.
5. composite bactericide according to claim 4, it is characterised in that: addition medicinal fungi tabasheer ethyl acetate extract
The concentration of extract is 0.06-0.14g/L.
6. composite bactericide according to claim 5, it is characterised in that: active constituent is that bamboo is red in the composite bactericide
The mass ratio of fungus beetle element, hypocrelline B and cystolycin A is (9-14): (1.5-3): (3-9) further includes metal ion
Ca2+Concentration is 0.045-0.055mol/L, metal ions M g2+Concentration is 0.035-0.045mol/L, medicinal fungi tabasheer acetic acid
The concentration of the extract at ethyl ester position is 0.06-0.14g/L.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201910248233.6A CN109924201B (en) | 2019-03-29 | 2019-03-29 | Compound bactericide of perylenequinone compound |
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| CN201910248233.6A CN109924201B (en) | 2019-03-29 | 2019-03-29 | Compound bactericide of perylenequinone compound |
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Cited By (2)
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| CN115443997A (en) * | 2022-10-13 | 2022-12-09 | 四川农业大学 | Plant growth regulator containing hypocrellin and application thereof |
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