CN116998497A - Application of citronellol and geraniol as agricultural bactericides and synergists in plant diseases - Google Patents
Application of citronellol and geraniol as agricultural bactericides and synergists in plant diseases Download PDFInfo
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- CN116998497A CN116998497A CN202310683216.1A CN202310683216A CN116998497A CN 116998497 A CN116998497 A CN 116998497A CN 202310683216 A CN202310683216 A CN 202310683216A CN 116998497 A CN116998497 A CN 116998497A
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- citronellol
- geraniol
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- bacteria
- pathogenic
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- QMVPMAAFGQKVCJ-UHFFFAOYSA-N citronellol Chemical compound OCCC(C)CCC=C(C)C QMVPMAAFGQKVCJ-UHFFFAOYSA-N 0.000 title claims abstract description 122
- GLZPCOQZEFWAFX-UHFFFAOYSA-N Geraniol Chemical compound CC(C)=CCCC(C)=CCO GLZPCOQZEFWAFX-UHFFFAOYSA-N 0.000 title claims abstract description 114
- 235000000484 citronellol Nutrition 0.000 title claims abstract description 61
- QMVPMAAFGQKVCJ-SNVBAGLBSA-N (R)-(+)-citronellol Natural products OCC[C@H](C)CCC=C(C)C QMVPMAAFGQKVCJ-SNVBAGLBSA-N 0.000 title claims abstract description 60
- JGQFVRIQXUFPAH-UHFFFAOYSA-N beta-citronellol Natural products OCCC(C)CCCC(C)=C JGQFVRIQXUFPAH-UHFFFAOYSA-N 0.000 title claims abstract description 60
- 239000005792 Geraniol Substances 0.000 title claims abstract description 57
- GLZPCOQZEFWAFX-YFHOEESVSA-N Geraniol Natural products CC(C)=CCC\C(C)=C/CO GLZPCOQZEFWAFX-YFHOEESVSA-N 0.000 title claims abstract description 57
- 229940113087 geraniol Drugs 0.000 title claims abstract description 57
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 48
- 201000010099 disease Diseases 0.000 title claims abstract description 24
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 24
- 239000003899 bactericide agent Substances 0.000 title claims abstract description 14
- 241000813090 Rhizoctonia solani Species 0.000 claims abstract description 16
- 239000000575 pesticide Substances 0.000 claims abstract description 11
- 241000221696 Sclerotinia sclerotiorum Species 0.000 claims abstract description 6
- 230000001580 bacterial effect Effects 0.000 claims description 38
- 240000007594 Oryza sativa Species 0.000 claims description 37
- 235000007164 Oryza sativa Nutrition 0.000 claims description 37
- 235000009566 rice Nutrition 0.000 claims description 37
- 241000894006 Bacteria Species 0.000 claims description 29
- 241000196324 Embryophyta Species 0.000 claims description 17
- 230000001717 pathogenic effect Effects 0.000 claims description 17
- 239000003814 drug Substances 0.000 claims description 15
- 241000223218 Fusarium Species 0.000 claims description 9
- 239000005868 Metconazole Substances 0.000 claims description 9
- 244000052616 bacterial pathogen Species 0.000 claims description 9
- XWPZUHJBOLQNMN-UHFFFAOYSA-N metconazole Chemical compound C1=NC=NN1CC1(O)C(C)(C)CCC1CC1=CC=C(Cl)C=C1 XWPZUHJBOLQNMN-UHFFFAOYSA-N 0.000 claims description 9
- 241000082085 Verticillium <Phyllachorales> Species 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 7
- 235000002566 Capsicum Nutrition 0.000 claims description 6
- 241000233866 Fungi Species 0.000 claims description 6
- 241000589634 Xanthomonas Species 0.000 claims description 6
- 241000223600 Alternaria Species 0.000 claims description 5
- 241000589771 Ralstonia solanacearum Species 0.000 claims description 5
- 241000123650 Botrytis cinerea Species 0.000 claims description 4
- 241000219112 Cucumis Species 0.000 claims description 4
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 claims description 4
- 240000008067 Cucumis sativus Species 0.000 claims description 4
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 claims description 4
- 241000233614 Phytophthora Species 0.000 claims description 4
- 241000589615 Pseudomonas syringae Species 0.000 claims description 4
- 241000233639 Pythium Species 0.000 claims description 4
- 241001123668 Verticillium dahliae Species 0.000 claims description 4
- 241000589652 Xanthomonas oryzae Species 0.000 claims description 4
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 claims description 4
- 230000003071 parasitic effect Effects 0.000 claims description 4
- 241001558929 Sclerotium <basidiomycota> Species 0.000 claims description 3
- WEFHSZAZNMEWKJ-KEDVMYETSA-N (6Z,8E)-undeca-6,8,10-trien-2-one (6E,8E)-undeca-6,8,10-trien-2-one (6Z,8E)-undeca-6,8,10-trien-3-one (6E,8E)-undeca-6,8,10-trien-3-one (6Z,8E)-undeca-6,8,10-trien-4-one (6E,8E)-undeca-6,8,10-trien-4-one Chemical compound CCCC(=O)C\C=C\C=C\C=C.CCCC(=O)C\C=C/C=C/C=C.CCC(=O)CC\C=C\C=C\C=C.CCC(=O)CC\C=C/C=C/C=C.CC(=O)CCC\C=C\C=C\C=C.CC(=O)CCC\C=C/C=C/C=C WEFHSZAZNMEWKJ-KEDVMYETSA-N 0.000 claims description 2
- 244000298697 Actinidia deliciosa Species 0.000 claims description 2
- 235000009436 Actinidia deliciosa Nutrition 0.000 claims description 2
- 240000008574 Capsicum frutescens Species 0.000 claims description 2
- 241001619326 Cephalosporium Species 0.000 claims description 2
- 241000222199 Colletotrichum Species 0.000 claims description 2
- 241000186216 Corynebacterium Species 0.000 claims description 2
- 241000116713 Ferula gummosa Species 0.000 claims description 2
- 241000223221 Fusarium oxysporum Species 0.000 claims description 2
- 244000068988 Glycine max Species 0.000 claims description 2
- 235000010469 Glycine max Nutrition 0.000 claims description 2
- 241000206581 Gracilaria Species 0.000 claims description 2
- 235000007688 Lycopersicon esculentum Nutrition 0.000 claims description 2
- 241000226677 Myceliophthora Species 0.000 claims description 2
- 244000061176 Nicotiana tabacum Species 0.000 claims description 2
- 235000002637 Nicotiana tabacum Nutrition 0.000 claims description 2
- 241000233679 Peronosporaceae Species 0.000 claims description 2
- 241000233616 Phytophthora capsici Species 0.000 claims description 2
- 241000233629 Phytophthora parasitica Species 0.000 claims description 2
- 241000589626 Pseudomonas syringae pv. tomato Species 0.000 claims description 2
- 241000698291 Rugosa Species 0.000 claims description 2
- 240000005319 Sedum acre Species 0.000 claims description 2
- 240000003768 Solanum lycopersicum Species 0.000 claims description 2
- 240000006394 Sorghum bicolor Species 0.000 claims description 2
- 235000011684 Sorghum saccharatum Nutrition 0.000 claims description 2
- 241000589636 Xanthomonas campestris Species 0.000 claims description 2
- 239000001390 capsicum minimum Substances 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 239000004864 galbanum Substances 0.000 claims description 2
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 claims description 2
- 241000894007 species Species 0.000 claims description 2
- 239000003905 agrochemical Substances 0.000 claims 1
- 230000005764 inhibitory process Effects 0.000 abstract description 25
- 238000012360 testing method Methods 0.000 abstract description 13
- 238000012827 research and development Methods 0.000 abstract description 2
- 230000002195 synergetic effect Effects 0.000 abstract 2
- RSNWORHVUOZYLT-UHFFFAOYSA-N 5-[[(2-sulfanylidene-3h-1,3,4-thiadiazol-5-yl)amino]methylamino]-3h-1,3,4-thiadiazole-2-thione Chemical compound S1C(=S)NN=C1NCNC1=NNC(=S)S1 RSNWORHVUOZYLT-UHFFFAOYSA-N 0.000 description 14
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 12
- 238000000338 in vitro Methods 0.000 description 11
- 239000002609 medium Substances 0.000 description 11
- 239000001963 growth medium Substances 0.000 description 10
- WJCNZQLZVWNLKY-UHFFFAOYSA-N thiabendazole Chemical compound S1C=NC(C=2NC3=CC=CC=C3N=2)=C1 WJCNZQLZVWNLKY-UHFFFAOYSA-N 0.000 description 10
- 241000207199 Citrus Species 0.000 description 9
- 235000020971 citrus fruits Nutrition 0.000 description 9
- 230000002401 inhibitory effect Effects 0.000 description 9
- 239000004308 thiabendazole Substances 0.000 description 9
- 229960004546 thiabendazole Drugs 0.000 description 9
- 235000010296 thiabendazole Nutrition 0.000 description 9
- 229940079593 drug Drugs 0.000 description 8
- 239000007788 liquid Substances 0.000 description 8
- 244000052769 pathogen Species 0.000 description 8
- 230000000694 effects Effects 0.000 description 7
- 238000012258 culturing Methods 0.000 description 6
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 5
- 239000010949 copper Substances 0.000 description 5
- 229910052802 copper Inorganic materials 0.000 description 5
- 230000012010 growth Effects 0.000 description 5
- 230000001965 increasing effect Effects 0.000 description 5
- 239000001965 potato dextrose agar Substances 0.000 description 5
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 4
- 239000006002 Pepper Substances 0.000 description 4
- 241000722363 Piper Species 0.000 description 4
- 235000016761 Piper aduncum Nutrition 0.000 description 4
- 235000017804 Piper guineense Nutrition 0.000 description 4
- 235000008184 Piper nigrum Nutrition 0.000 description 4
- 244000061458 Solanum melongena Species 0.000 description 4
- 235000002597 Solanum melongena Nutrition 0.000 description 4
- 241000209140 Triticum Species 0.000 description 4
- 235000021307 Triticum Nutrition 0.000 description 4
- 240000006365 Vitis vinifera Species 0.000 description 4
- 235000014787 Vitis vinifera Nutrition 0.000 description 4
- 241001272684 Xanthomonas campestris pv. oryzae Species 0.000 description 4
- 244000000005 bacterial plant pathogen Species 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 231100000331 toxic Toxicity 0.000 description 4
- 230000002588 toxic effect Effects 0.000 description 4
- 230000002538 fungal effect Effects 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- 239000012747 synergistic agent Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 208000035143 Bacterial infection Diseases 0.000 description 2
- 241000228456 Leptosphaeria Species 0.000 description 2
- 241000589655 Xanthomonas citri Species 0.000 description 2
- 230000002411 adverse Effects 0.000 description 2
- 238000012271 agricultural production Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 235000021186 dishes Nutrition 0.000 description 2
- 230000000670 limiting effect Effects 0.000 description 2
- 238000009630 liquid culture Methods 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000003032 phytopathogenic effect Effects 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 231100000419 toxicity Toxicity 0.000 description 2
- 230000001988 toxicity Effects 0.000 description 2
- GLZPCOQZEFWAFX-JXMROGBWSA-N (E)-Geraniol Chemical compound CC(C)=CCC\C(C)=C\CO GLZPCOQZEFWAFX-JXMROGBWSA-N 0.000 description 1
- 239000005740 Boscalid Substances 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 241000257303 Hymenoptera Species 0.000 description 1
- 241001330975 Magnaporthe oryzae Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 208000031888 Mycoses Diseases 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 206010053615 Thermal burn Diseases 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 230000003385 bacteriostatic effect Effects 0.000 description 1
- WYEMLYFITZORAB-UHFFFAOYSA-N boscalid Chemical compound C1=CC(Cl)=CC=C1C1=CC=CC=C1NC(=O)C1=CC=CN=C1Cl WYEMLYFITZORAB-UHFFFAOYSA-N 0.000 description 1
- 229940118790 boscalid Drugs 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000003113 dilution method Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000001804 emulsifying effect Effects 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000002480 mineral oil Substances 0.000 description 1
- 235000010446 mineral oil Nutrition 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 230000002085 persistent effect Effects 0.000 description 1
- 239000003175 pesticide synergist Substances 0.000 description 1
- 244000000003 plant pathogen Species 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 229920001296 polysiloxane Polymers 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 239000000375 suspending agent Substances 0.000 description 1
- 230000003313 weakening effect Effects 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N49/00—Biocides, pest repellants or attractants, or plant growth regulators, containing compounds containing the group, wherein m+n>=1, both X together may also mean —Y— or a direct carbon-to-carbon bond, and the carbon atoms marked with an asterisk are not part of any ring system other than that which may be formed by the atoms X, the carbon atoms in square brackets being part of any acyclic or cyclic structure, or the group, wherein A means a carbon atom or Y, n>=0, and not more than one of these carbon atoms being a member of the same ring system, e.g. juvenile insect hormones or mimics thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/72—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms
- A01N43/82—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with three ring hetero atoms
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Forests & Forestry (AREA)
- Agronomy & Crop Science (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Dentistry (AREA)
- Ecology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Insects & Arthropods (AREA)
- Botany (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The application discloses application of citronellol and geraniol as agricultural bactericides and synergists in plant diseases, and belongs to the technical field of plant disease control. The application discovers that the inhibition rate of citronellol to the Rhizoctonia solani is 55.2% at 25mg/L, and the inhibition rate of geraniol to the Sclerotinia sclerotiorum is 80.2% at 25mg/L. The bactericidal activity of BT and citronellol to Xoo is best when being compounded and used in a ratio of 1:32, the synergistic multiple is improved by 4 times compared with BT, the bactericidal activity of BT and geraniol to Xoo is best when being compounded and used in a ratio of 1:2, and the synergistic multiple is improved by 5 times compared with BT. The application expands the application of citronellol and geraniol, tests the antibacterial activity of the citronellol and geraniol, and provides an important scientific basis for the research and development and creation of novel plant-derived pesticides.
Description
Technical Field
The application relates to the technical field of plant disease control, in particular to application of citronellol and geraniol as agricultural bactericides and synergists in plant diseases.
Background
Plant diseases are common diseases in agricultural production, and have attracted considerable attention in the past decades due to their great threat to agricultural production. Such as bacterial leaf blight of rice and citrus canker are two common bacterial diseases caused by pathogenic varieties of the gram-negative bacteria xanthomonas oryzae (Xanthomonas oryzae, xao) and Xanthomonas citri pathogenic varieties of the genus Xanthomonas (Xanthomonas on opispv, citri, xac), respectively. Bacterial leaf blight of rice can occur at various stages of rice growth, which results in at least 10-50% yield loss per year in countries where rice is planted. Citrus canker is an extremely persistent disease that can cause damage to the leaves, stems and fruits of citrus trees, with a significant loss of citrus yield each year. The long-term use of traditional commercial bactericides such as thiabendazole, metconazole and the like not only increases the drug resistance of plant pathogenic bacteria, but also has influence on the environment and the safety of plants. Therefore, the development of the novel pesticide with low cost, low toxicity, high efficiency and high selectivity has very important significance.
A bactericidal potentiator is a substance capable of enhancing the activity of a bactericidal agent, and is generally not bactericidal by itself or is weak. These synergists generally have low surface tension, good spreadability, permeability or emulsifying dispersibility, etc., such as silicone-based surfactants and mineral oil. They can enhance the wetting, adhesion and spreading ability of the liquid medicine on the plant surface, thereby improving the effective utilization rate of the medicine and remarkably improving the prevention effect of the pesticide. However, conventional chemical pesticide synergists also suffer from several disadvantages, such as: the pollution to soil and water sources is caused, and the adverse effect is generated on an ecological system, so that the biodiversity and ecological balance are influenced; part of the bactericidal potentiator or its metabolites may also remain in crops and if used improperly, these residues may pose health risks to humans and animals; long-term use of the same bactericidal synergist may lead to resistance of pathogens, thereby weakening the sensitivity of the pathogens to the chemicals, which may exacerbate disease spread and aggravate damage of the diseases to crops; the cost of the sterilization synergistic agent is relatively high, and the production cost of farmers can be increased, so that the income of farmers is reduced; in addition, bactericidal synergists may have adverse effects on non-target organisms, including effects on beneficial insects, bees and other beneficial organisms. Therefore, the sterilization synergistic agent should be used by paying attention to the use method, follow the use instruction and find more environment-friendly and safer alternatives as far as possible to control diseases and improve crop yield.
Disclosure of Invention
The application aims to provide application of citronellol and geraniol as agricultural bactericides and synergists in plant diseases so as to solve the problems in the prior art.
In order to achieve the above object, the present application provides the following solutions:
the application provides a bactericide which comprises citronellol and/or geraniol.
Preferably, the ratio of citronellol to geraniol is 1:60.
preferably, the citronellol has the formula C 10 H 20 O,3, 7-dimethyloct-6-en-1-ol, having the structure:
preferably, the median inhibitory concentration (EC of citronellol against rice bacterial blight bacteria 50 ) 9.88mg/L.
Preferably, the median inhibitory concentration (EC of citronellol against rice bacterial leaf spot 50 ) 14.8mg/L.
Preferably, the inhibition rate of citronellol to Rhizoctonia solani is 55.2%.
Preferably, the geraniol has the formula C 10 H 18 O, (E) -3, 7-dimethyloct-2, 6-dien-1-ol, has the structure:
preferably, the median inhibitory concentration (EC of geraniol against Rhizoctonia solani 50 ) 23.9mg/L.
Preferably, the median inhibitory concentration (EC of geraniol against the bacterial species Pyricularia oryzae 50 ) Greater than 50mg/L.
Preferably, the median inhibitory concentration (EC of geraniol against Leptodermia citri 50 ) Greater than 50mg/L.
The application also provides a synergist of the bactericide, which comprises citronellol and/or geraniol.
The application also provides a pesticide for treating plant diseases, which comprises the synergist.
Preferably, the composition further comprises a medicament having a bactericidal effect.
Preferably, the agent having a bactericidal effect includes bismerthiazol.
Preferably, the ratio of the tolazol to the citronellol is 1:32.
Preferably, the ratio of the bismerthiazol to the geraniol is 1:2.
The application also provides a method for preventing and controlling plant diseases, and the pesticide is used for preventing and controlling the diseases of crops.
It is preferred that the composition of the present application, the plant diseases comprise rice bacterial leaf blight pathogenic bacteria, rice xanthomonas oryzae fine strip pathogenic varieties, xanthomonas rugosa, xanthomonas campestris pathogenic varieties, pseudomonas syringae tomato leaf spot pathogenic varieties, pseudomonas syringae kiwifruit pathogenic varieties, ralstonia solanacearum, pseudomonas syringae cucumber angular leaf spot pathogenic varieties, pseudomonas solanacearum, xanthomonas sojae soybean pathogenic varieties or corynebacterium michiganensis tomato canker pathogenic bacteria and black and white verticillium, verticillium dahliae, phytophthora parasitica, black rot fungi, rhizoctonia solani, roseo disc two spores, large spot convex navel vermicularia, stripe rust bacteria, cucumber gray mold, parasitic downy mildew, yellow earth mould, phytophthora root rot, tobacco epidemic, sclerotium disc bacteria, melon pythium, heteromyces, pythium, rhizoctonia solani, fusarium species, galbanum, melon mold, myces, myceliophthora, parasitic fungus, 62, phytophthora capsicum, 38. Gracilaria, phytophthora capsici, and other species, diseases caused by Botrytis cinerea, fusarium oxysporum, sclerotinia sclerotiorum, verticillium dahliae, alternaria colletotrichum, alternaria sinensis, and Cephalosporium sorghum.
The application discloses the following technical effects:
according to the application, citronellol and geraniol are used as bactericides to prevent and treat plant bacteria and fungal diseases for the first time, and compared with the traditional bactericides, the citronellol and geraniol used as natural products have the advantages of being green, safe, and capable of being degraded by soil microorganisms. The application verifies the half inhibition concentration (EC) of citronellol to rice bacterial blight bacteria through experiments 50 ) 9.88mg/L; half inhibition concentration (EC) of geraniol on rice bacterial blight 50 ) At 23.9mg/L, the median inhibitory concentration (EC) of citronellol against said rice bacterial leaf scald bacteria 50 ) 14.8mg/L; the median inhibitory concentration (EC of geraniol against the rice bacterial leaf spot pathogen 50 ) Greater than 50mg/L, the median inhibitory concentration (EC) of citronellol against said citrus canker pathogen 50 ) 33.7 mg-L is; the median inhibitory concentration (EC 50 ) The inhibition rate of citronellol to the grape vine canker germ is 20.24%, the inhibition rate of citronellol to the rice sheath blight germ is 55.21%, the inhibition rate of citronellol to the pepper fusarium wilt germ is 9.88%, and the inhibition rate of citronellol to the eggplant verticillium wilt germ is 43.64%; the inhibition rate of geraniol to the grape vine cavity germ is 11.01%, the inhibition rate of the rice sheath blight germ is 31.86%, the inhibition rate of the pepper fusarium wilt germ is 7.41%, the inhibition rate of the rape sclerotium germ is 80.24%, and the inhibition rate of the eggplant verticillium wilt is 18.34%.
The application discovers that BT and citronellol have the best bactericidal activity on the Xoo when being compounded and used in a ratio of 1:32, and half inhibition concentration (EC 50 ) At 9.87mg/L (4-fold increase in synergy compared with BT), BT and geraniol have the best bactericidal activity on the Xoo when being compounded and used in a ratio of 1:2, and half inhibition concentration (EC) 50 ) 7.91mg/L (5-fold increase in synergy compared to BT). The application expands the application of citronellol and geraniol, tests the antibacterial activity of the citronellol and geraniol, and provides an important scientific basis for the research and development and creation of novel plant-derived pesticides.
Detailed Description
Various exemplary embodiments of the application will now be described in detail, which should not be considered as limiting the application, but rather as more detailed descriptions of certain aspects, features and embodiments of the application.
It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application. In addition, for numerical ranges in this disclosure, it is understood that each intermediate value between the upper and lower limits of the ranges is also specifically disclosed. Every smaller range between any stated value or stated range, and any other stated value or intermediate value within the stated range, is also encompassed within the application. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.
Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present application. All documents mentioned in this specification are incorporated by reference for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.
It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the application described herein without departing from the scope or spirit of the application. Other embodiments will be apparent to those skilled in the art from consideration of the specification of the present application. The specification and examples of the present application are exemplary only.
As used herein, the terms "comprising," "including," "having," "containing," and the like are intended to be inclusive and mean an inclusion, but not limited to.
The technical scheme of the application is conventional in the field, and the reagents or raw materials are purchased from commercial sources or are disclosed.
In the application, citronellol and geraniol are purchased from Shanghai Bi to get medical science and technology Co., ltd; the rice bacterial leaf blight bacteria used in the application are provided by a subject group of Zhou Mingguo teacher at Nanjing university of agriculture; the citrus canker pathogen is preserved by a Yang Song teacher subject group; the bacterial class-II rice germs are provided by the university of Shanghai traffic Lifang teacher subject group.
Example 1 measurement of the effective Medium concentration of citronellol, geraniol, against plant pathogens by turbidity 50
The test subjects were rice bacterial leaf blight bacteria (Xoo), citrus canker bacteria (Xac) and rice bacterial leaf spot bacteria (Xoc). Copper thiabendazole (20% wettable suspension) and metconazole (90% stock) served as test controls, and DMSO was dissolved in the medium as a blank.
1.1 respectively inoculating bacterial leaf blight bacteria, citrus canker bacteria and bacterial leaf spot bacteria of rice into NB culture medium, and shake culturing at 28deg.C in 180rpm constant temperature shaker until logarithmic phase is reserved.
1.2 citronellol, geraniol, copper thiabendazole (20% wettable suspension) and metconazole (90% crude drug) were prepared by a double dilution method to 100, 50, 25, 12.5,6.25mg/L respectively, of NB liquid medium containing citronellol and geraniol at different concentrations, and 5mL of each was added to test tubes.
1.3 adding 40 mu L of NB liquid culture medium containing phytopathogenic bacteria into the test tube filled with the medicaments with different concentrations in the step 1.2, respectively, shaking in a constant temperature shaking table at 28 ℃ and 180rpm, culturing the bacterial leaf blight of rice for 36h, culturing the bacterial canker of citrus for 48h, and culturing the bacterial leaf spot of rice for 36h.
1.4 measurement of OD of bacterial solutions of respective concentrations on a spectrophotometer 595 Values, and additionally determining the OD of corresponding concentrations of toxic sterile NB liquid medium 595 Values.
Corrected OD = bacteria-containing medium OD-sterile medium OD
Inhibition ratio = [ (corrected control culture medium bacterial liquid OD value-corrected toxic culture medium OD value)/corrected control culture medium bacterial liquid OD value ] ×100
The experimental results are shown in table 1.
TABLE 1 EC of citronellol, geraniol against plant pathogenic bacteria, rhizoctonia solani, leptosphaeria citri and Leptosphaeria oryzae 50 Value of
As can be seen from Table 1, citronellol exhibits excellent in vitro antibacterial activity against Rhizoctonia solani, and EC thereof 50 =9.88 mg/L, its in vitro antibacterial activity is significantly better than that of the commercial control drug, metconazole (EC 50 =31.1 mg/L) and thiabendazole (EC 50 >50 mg/L); citronellol has better in-vitro antibacterial activity on citrus canker pathogens, and EC thereof 50 =33.7 mg/L, its in vitro antibacterial activity is significantly better than that of the commercial control drug, and its in vitro antibacterial activity is better than that of the commercial drug (EC) 50 >50mg/L) and copper thiabendazole (EC) 50 >50 mg/L); citronellol exhibits excellent in vitro antibacterial activity against bacterial leaf streaks in rice, and its EC 50 =14.8 mg/L, which has significantly better in vitro antibacterial activity than the commercial control drug, metconazole (EC 50 >50 mg/L) and copper thiabendazole (EC 50 >50 mg/L). Whereas geraniol shows better in-vitro antibacterial activity on rice ralstonia solanacearum and EC thereof 50 =23.9 mg/L, its in vitro antibacterial activity is significantly better than that of the commercial control drug, metconazole (EC 50 =31.1 mg/L) and thiabendazole (EC 50 >50mg/L)。
Example 2 EC of citronellol and geraniol as a synergistic agent against plant pathogenic bacteria Rhizoctonia solani and Rhizoctonia solani 50 Value of
The test subjects were rice bacterial leaf blight bacteria (Xoo) and rice bacterial leaf streak bacteria (Xoc). Copper thiabendazole (20% wettable suspension) and metconazole (90% stock) served as test controls, and DMSO was dissolved in the medium as a blank.
1.1 respectively inoculating bacterial leaf blight bacteria and bacterial leaf spot bacteria of rice into NB culture medium, and shake culturing at 28deg.C in 180rpm constant temperature shaker until logarithmic growth phase is reserved.
1.2, the citronellol and the geraniol are mixed according to different proportions, and the Bismerthiazol (BT) is mixed with the citronellol and the geraniol respectively, and is added into a test tube together with the thiabendazole (20% wettable suspending agent) and the bismerthiazol (90% raw material) by 5mL respectively.
1.3 adding 40 mu L of NB liquid culture medium containing plant disease bacteria into the test tube with the medicines according to different compound proportions in the step 1.2, and culturing the bacterial leaf blight bacteria of rice for 36h and the bacterial leaf streak of rice for 36h by shaking in a constant temperature shaking table at 28 ℃ and 180 rpm.
1.4 measurement of OD of bacterial solutions of respective concentrations on a spectrophotometer 595 Values, and additionally determining the OD of corresponding concentrations of toxic sterile NB liquid medium 595 Values.
Corrected OD = bacteria-containing medium OD-sterile medium OD
Inhibition ratio = [ (corrected control culture medium bacterial liquid OD value-corrected toxic culture medium OD value)/corrected control culture medium bacterial liquid OD value ] ×100
The experimental results are shown in table 2.
TABLE 2 EC of citronellol and geraniol as synergists against plant pathogenic bacteria Rhizoctonia solani and Rhizoctonia solani 50 Value of
As can be seen from Table 2, the citronellol and geraniol can obviously improve the activity of the citronellol on bacterial blight of rice and bacterial leaf streak of rice at a certain ratio. Under the two proportioning concentrations of citronellol and geraniol of 1:1 and 1:20, the EC of citronellol on rice bacterial blight bacteria is realized 50 Decreasing with increasing proportion of geraniol addition indicates increasing toxicity of the compounded drug. EC of citronellol and geraniol to bacterial blight of rice at a ratio of 1:40 50 Slightly higher than the ratio of 1:60; under the two ratio concentrations of citronellol and geraniol of 1:10 and 1:60, the EC of citronellol on rice bacterial leaf streak bacteria 50 As the adding proportion of the geraniol is increased, the toxicity of the mixed medicament is increased, and the EC of the citronellol and the geraniol on the rice bacterial leaf spot bacteria is improved at the proportion of 1:20 50 Slightly higher than the ratio of 1:40. BT and citronellol have the most obvious bactericidal activity on bacterial blight of rice at a ratio of 1:32. EC of BT and geraniol to bacterial blight of rice at a ratio of 1:1, 1:2 and 1:4 50 The bactericidal activity difference between the 1:4 and 1:2 ratios is not obvious along with the increase of the geraniol addition proportion, which indicates that the synergy of the mixed medicament is not obvious; BT and geraniol are proportioned at a ratio of 1:16, and EC of BT and geraniol to bacterial blight bacteria of rice 50 Slightly higher than the mixture ratio of 1:8 and 1:32. The bactericidal activity of BT and geraniol on rice bacterial strip spot bacteria is not obvious at the four proportioning concentrations of 1:1, 1:2, 1:4 and 1:8.
Example 3 test of inhibition of in vitro antibacterial efficacy of citronellol, geraniol against phytopathogenic fungi Using mycelium growth Rate method
The test subjects of the fungus, botrytis cinerea, rhizoctonia solani, sclerotinia sclerotiorum, verticillium eggplant verticillium and Gibberella wheat take DMSO in sterile distilled water as negative control, and boscalid as positive control.
1.1 citronellol and geraniol were dissolved in DMSO (1.0 mL), respectively, then added to 9.0mL of sterile water containing Tween 20 (1%) and then mixed with potato dextrose agar (PDA, 90.0 mL) at a concentration of 25mg/L.
1.2 the stock solution was transferred uniformly to three 9cm diameter petri dishes.
1.3 mycelium dishes of approximately 4mm diameter were then cut from the medium and inoculated aseptically in the middle of the PDA plates.
1.4 plates after inoculation were incubated at 27.+ -. 1 ℃ for 3-5 days with 3 replicates per treatment condition.
1.5 radial growth of fungal colonies was measured and data were statistically analyzed. The bacteriostatic effect was calculated as = [ (C-T)/(C-0.4) ]x100%, where C is the diameter of fungal growth on untreated PDA, T is the diameter of fungal growth on treated PDA, and I is the rate of inhibition.
The experimental results are shown in table 3.
TABLE 3 inhibition of in vitro antibacterial efficacy of citronellol and geraniol against plant pathogenic fungi Botrytis cinerea, rhizoctonia solani, verticillium eggplant and Alternaria wheat (percent)
As can be seen from Table 3, at 25mg/L, citronellol exhibits excellent antibacterial activity against Rhizoctonia solani and Verticillium eggplant verticillium with inhibition ratio of 55.2% and 43.6%; the citronellol has no obvious antibacterial activity on the grape vine canker pathogens and the pepper fusarium wilt pathogens, and the inhibition rates are 20.2 percent and 9.9 percent respectively; citronellol has no antibacterial activity against Sclerotinia sclerotiorum and Gibberella wheat. The geraniol has excellent antibacterial activity on sclerotinia sclerotiorum, and the inhibition rate is 80.2%; the antibacterial activity of geraniol on the grape vine cavity germs, the rice sheath blight germs, the pepper fusarium wilt germs and the eggplant verticillium wilt germs is not obvious, and the inhibition rates are 11.0%,31.9%,7.4% and 18.3% respectively; geraniol has no antibacterial activity against gibberella wheat germ.
The above embodiments are only illustrative of the preferred embodiments of the present application and are not intended to limit the scope of the present application, and various modifications and improvements made by those skilled in the art to the technical solutions of the present application should fall within the protection scope defined by the claims of the present application without departing from the design spirit of the present application.
Claims (10)
1. A bactericide comprising citronellol and/or geraniol.
2. The bactericide according to claim 1, characterized in that the ratio of citronellol to geraniol is 1:60.
3. A synergist of a bactericide, which is characterized by comprising citronellol and/or geraniol.
4. A pesticide for treating plant diseases, comprising the synergist of claim 3.
5. A pesticide according to claim 4, further comprising a medicament having a bactericidal effect.
6. A pesticide according to claim 5, wherein the agent having a bactericidal effect comprises metconazole.
7. A pesticide according to claim 6, characterised in that the ratio of metconazole to citronellol is 1:32.
8. A pesticide according to claim 6, characterised in that the ratio of the leaf-subtilis to the geraniol is 1:2.
9. A method for controlling plant diseases, characterized in that the agricultural chemical according to any one of claims 4 to 8 is used for controlling plant diseases on crops.
10. The use according to claim 9, wherein, the plant diseases comprise rice bacterial leaf blight pathogenic bacteria, rice xanthomonas oryzae fine strip pathogenic varieties, xanthomonas rugosa, xanthomonas campestris pathogenic varieties, pseudomonas syringae tomato leaf spot pathogenic varieties, pseudomonas syringae kiwifruit pathogenic varieties, ralstonia solanacearum, pseudomonas syringae cucumber angular leaf spot pathogenic varieties, pseudomonas solanacearum, xanthomonas sojae soybean pathogenic varieties or corynebacterium michiganensis tomato canker pathogenic bacteria and black and white verticillium, verticillium dahliae, phytophthora parasitica, black rot fungi, rhizoctonia solani, roseo disc two spores, large spot convex navel vermicularia, stripe rust bacteria, cucumber gray mold, parasitic downy mildew, yellow earth mould, phytophthora root rot, tobacco epidemic, sclerotium disc bacteria, melon pythium, heteromyces, pythium, rhizoctonia solani, fusarium species, galbanum, melon mold, myces, myceliophthora, parasitic fungus, 62, phytophthora capsicum, 38. Gracilaria, phytophthora capsici, and other species, diseases caused by Botrytis cinerea, fusarium oxysporum, sclerotinia sclerotiorum, verticillium dahliae, alternaria colletotrichum, alternaria sinensis, and Cephalosporium sorghum.
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