CN109924201B - Compound bactericide of perylenequinone compound - Google Patents
Compound bactericide of perylenequinone compound Download PDFInfo
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- CN109924201B CN109924201B CN201910248233.6A CN201910248233A CN109924201B CN 109924201 B CN109924201 B CN 109924201B CN 201910248233 A CN201910248233 A CN 201910248233A CN 109924201 B CN109924201 B CN 109924201B
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Abstract
The invention provides a compound bactericide containing perylenequinone compounds, which comprises the active ingredients of hypocrellin A, hypocrellin B and elsinochrome A, wherein the mass ratio of the hypocrellin A to the hypocrellin B to the elsinochrome A is (9-14): (1.5-3): (3-9). The compound bactericide containing the perylenequinone compound can play a good bactericidal role, has a wide bactericidal spectrum, and solves the problem of serious resistance of the existing bactericide; and can obtain better pesticide effect under a very small dosage, has little residue in crops and cannot cause pollution to the environment; the metal ions and the extract of the medicinal fungi are also added, so that the compound bactericide can play a role of guiding drugs, can promote the absorption and conduction of the active ingredients of the pesticide and the nutrient ingredients in the soil by the crops, improve the disease resistance of the crops, enhance the drug effect, improve the physique of the crops, enhance the metabolism of the crops and improve the disease resistance effect.
Description
Technical Field
The invention belongs to the field of pesticides, and particularly relates to a compound bactericide of a perylenequinone compound.
Background
With the continuous development of the pesticide industry, low-toxicity, high-efficiency and low-residue pesticides are more and more widely concerned by society, and the development of green and environment-friendly pesticides becomes a main way for researching and developing new pesticides.
Disclosure of Invention
The invention aims to provide an optimal formula for preventing and controlling the perylenequinone compound of the phytopathogen, so that the perylenequinone compound can be used for preparing a bactericide and preventing and controlling the phytopathogen; the invention also aims to provide a method for obtaining better pesticide effect of the compound bactericide containing the perylenequinone compound under the condition of small using dosage, and the compound bactericide has little residue in crops and does not cause pollution to the environment.
In order to get rid of the current situation that the traditional pesticide for treating pathogenic bacteria has large dosage and poor effect, a novel pesticide with small dosage, safety to people and livestock and no pesticide residue needs to be developed so as to prevent and treat the harmful pathogenic bacteria in Chinese herbal medicines and crops. By utilizing the characteristics of the perylenequinone compound and an optimization method, the invention provides an optimal formula for preventing and controlling the perylenequinone compound of plant pathogenic bacteria, so that the perylenequinone compound can be used for preparing bactericides and preventing and controlling the plant pathogenic bacteria.
The technical scheme is as follows: a compound bactericide containing perylenequinone compounds comprises the active ingredients of hypocrellin A, hypocrellin B and elsinochrome A, wherein the mass ratio of the hypocrellin A to the hypocrellin B to the elsinochrome A is (9-14): (1.5-3): (3-9).
As an improvement, a synergist is also added into the compound bactericide, and the synergist comprises metal ion Ca2+And Mg2+。
As an improvement, the metal ion Ca2+The concentration is 0.045-0.055mol/L, and metal ion Mg2+The concentration is 0.035-0.045 mol/L.
As an improvement, the synergist in the compound bactericide also comprises an extract of an ethyl acetate part of medicinal fungi concretio silicea Bambusae seu schizostachyi.
As an improvement, the concentration of the extract of the part added with medicinal fungi concretio silicea Bambusae seu Schizostachyi ethyl acetate is 0.06-0.14 g/L.
As an improvement, the active ingredients in the compound bactericide are hypocrellin A, hypocrellin B and elsinochrome A in a mass ratio of (9-14): (1.5-3): (3-9) further comprising a metal ion Ca2+The concentration is 0.045-0.055mol/L, and metal ion Mg2+The concentration is 0.035-0.045mol/L, and the concentration of the extract of the medicinal fungus concretio silicea Bambusae seu Schizostachyi ethyl acetate part is 0.06-0.14 g/L.
Has the advantages that: the invention is a significant research work for exploring a method for a compound bactericide containing perylenequinone compounds and application thereof in order to exert the comprehensive benefits of the perylenequinone compounds. The compound bactericide has good bactericidal effect and wide bactericidal spectrum, and solves the problem of serious resistance of the existing bactericide.
Compared with the traditional method, the compound bactericide containing the perylenequinone compound can obtain better pesticide effect under a very small using dose, has little residue in crops, cannot cause pollution to the environment, and is a novel pesticide; the plant extract is added into the preferable formula of the compound bactericide, and the synergist can play a role of guiding drugs in the compound bactericide, can promote the absorption and conduction of active ingredients of pesticides and nutrient ingredients in soil by crops, improve the disease resistance of the crops, enhance the drug effect, improve the physique of the crops, enhance the metabolism of the crops and improve the disease resistance effect.
The foregoing is a summary of the present invention, and in order to provide a clear understanding of the technical means of the present invention and to be implemented in accordance with the present specification, the following is a preferred embodiment of the present invention and is described in detail below.
Detailed Description
The following examples are given to further illustrate the embodiments of the present invention. The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention.
The perylenequinone compound is a derivative of 4, 9-dihydroxy-3, 10-perylenequinone, is a novel pigment extracted and separated from some fungi of Ascomycetes and Deuteromycetes, and is a substance with photosensitive activity. The perylenequinone compound mainly comprises hypocrellin and elsinochrome, and the hypocrellin has the effects of resisting inflammation, easing pain, resisting bacteria, killing tumor cells by light-sensitive radiation, relieving toxic and side effects, inhibiting granulation tissue hyperplasia and the like. Elsinoe has effects in killing HIV virus, herpes simplex virus and Sindbis virus, and can be used for treating dermatoses.
Example 1
Research on influence of mass ratio of various perylenequinones compounds on antibacterial activity of compound bactericide
1. Experimental materials and methods
1.1 sources of Material
The representative pathogens in root rot, Fusarium solani (Fusarium solani) and Bacillus subtilis, are all deposited in the biopharmaceutical research laboratory of Bin institute. PDA solid medium, PD liquid medium, ordinary broth solid medium and the like are prepared according to a conventional method and then sterilized.
1.2 preparation of the paper sheet
And (3) soaking a filter paper sheet (8mm) in dimethyl sulfoxide solution containing different perylene quinone compounds for a while, and storing the filter paper sheet in the dark for later use.
1.3 measurement of Activity of Complex Fungicide
According to the aseptic operation protocol, 100 mu L of each tested bacterial suspension is respectively sucked, added to different plate culture media and evenly coated. And (3) flatly pasting filter paper sheets which are sterilized, dried and soaked with the perylenequinone compound on a bacterium-containing culture medium. After the treatment group is cultured in an incubator at 26 ℃ or 35 ℃ for 48 hours or 24 hours, the existence and the level of the activity of the compound bactericide are represented by the generation or the absence and the size of the inhibition zone. The larger the difference between the diameter of the inhibition zone and the diameter of the paper sheet is, the better the activity of the compound bactericide is.
2. Results of the experiment
2.1 Effect of the Mass ratios of the various Perylenequinone Compounds on the bacteriostatic Activity
The invention optimizes the mass ratio of the three perylenequinone compounds, takes the size of the antibacterial effect as an optimization index, finds that 100 mu L fusarium solani suspension cultured for 36 hours is added to a common broth culture medium, and when the mass ratio of the hypocrellin A, hypocrellin B and the elsinochrome is (9-14): (1.5-3): (3-9), the culture temperature in the incubator is 26 ℃, the culture time is 48h, and the inhibition effect is better.
Example 2
Optimization experiment result of metal ions and concentration of antibacterial activity related factors of compound bactericide
1. Experimental materials and methods
1.1 sources of Material
The representative pathogens in root rot, Fusarium solani (Fusarium solani) and Bacillus subtilis, are all deposited in the biopharmaceutical research laboratory of Bin institute.
PDA solid medium, PD liquid medium, ordinary broth solid medium and the like are prepared according to a conventional method and then sterilized.
1.2 preparation of the paper sheet
And (3) soaking a filter paper sheet (8mm) in dimethyl sulfoxide solution containing different perylene quinone compounds for a while, and storing the filter paper sheet in the dark for later use.
1.3 measurement of Activity of Complex Fungicide
According to the aseptic operation protocol, 100 mu L of each tested bacterial suspension is respectively sucked, added to different plate culture media and evenly coated. And (3) flatly pasting filter paper sheets which are sterilized, dried and soaked with the perylenequinone compound on a bacterium-containing culture medium. After the treatment group is cultured in an incubator at 26 or 35 ℃ for 48 or 24 hours, the existence and the level of the activity of the compound bactericide are represented by the generation or the absence and the size of the inhibition zone. The larger the difference between the diameter of the inhibition zone and the diameter of the paper sheet is, the better the activity of the compound bactericide is.
1.4 determination of bacteriostasis spectrum of compound bactericide on plant pathogenic fungi
The inhibition effect of the compound bactericide on pathogenic bacteria of test plants is measured by adopting a hypha growth rate method. And (3) sucking 50 mu g/mL of compound bactericide dimethyl sulfoxide solution, adding the mixture into the PDA culture medium, uniformly mixing, and pouring the mixture into a flat plate to be placed into a culture dish with the diameter of 9 cm. Control is PDA medium containing equal amount of dimethyl sulfoxide and blank is PDA medium without any reagent added. Each treatment was repeated 3 times, and a 4mm diameter cake of a pathogen bacteria which had been vigorously grown was inoculated into each of the above culture media. Sealing the inoculated culture medium with a preservative film, and culturing for 2 days in an illumination incubator at 26 ℃. All treatment dishes were removed and the diameter of each colony was measured, and the diameter of each colony was measured by the cross method.
The hyphal growth inhibition rate was calculated using the following formula:
2. results of the experiment
2.1 Effect of various Metal ions on the bacteriostatic Activity
The hypocrellin A, hypocrellin B and elsinochrome all have certain bacteriostatic effects, and how to exert the bacteriostatic effects of the hypocrellin A, hypocrellin B and elsinochrome to the maximum degree is realizedAs an optimization index. At a metal ion concentration of 0.05mol/L, K was detected+，Na+Ca2+，Mg2+And Al3+The inhibition rate of Fusarium solani is found to be monovalent metal ion K+And Na+The activity of the perylene quinone compound for inhibiting fusarium solani is not greatly influenced; it is found that 100 mul of Fusarium solani cultured for 36h is added to PDA culture medium, and divalent metal ion Ca is added into perylenequinone compound2+And Mg2+Has certain promotion effect on the antibacterial activity of the perylenequinone compounds, the inhibition rate reaches 94 percent and 93 percent, and Al3+The influence on the antibacterial activity of the perylene quinone compound is small.
TABLE 1 Effect of various Metal ions on Fusarium solani
| Metal ion | K+ | Na+ | Ca2+ | Mg2+ | Al3+ |
| Inhibition rate | 67％ | 78％ | 94％ | 93％ | 84％ |
2.2 Effect of Metal ion concentration on bacteriostatic Activity
Hypocrellin A, hypocrellin B and elsinochrome all have certain antibacterial effect, and divalent metal ion Ca is added into the hypocrellin A, hypocrellin B and elsinochrome2+And Mg2+The invention can enhance the bacteriostatic effect and give full play to the bacteriostatic effect, and the metal ion Ca is added into the perylenequinone compound2+And Mg2+The concentration of the bacillus subtilis is researched, the size of the bacteriostatic effect is taken as an optimization index, the addition of 100 mu L of fusarium solani cultured for 36 hours to a common PDA culture medium is found, and Ca is found2+The concentration is 0.045-0.055mol/L, and metal ion Mg2+The concentration is 0.035-0.045mol/L, the inhibition effect of the perylenequinone compound is obvious, and the stability of the cell membrane is reduced, so that the indicating bacteria is more sensitive to the bacteriostasis of the perylenequinone compound. At the same time, it was found that when the metal ion Ca2+And Mg2+At a concentration of 0.05mol/L Ca2+，0.04mol/L Mg2+When the perylenequinone compound is put into an incubator at 26 ℃ for culturing for 48 hours, the inhibition effect of the perylenequinone compound is the best. When ion is Ca2+And Mg2+The concentration is 0.05mol/L, and the survival rate of the fusarium solani is lowest when the concentration is 0.04mol/L and is reduced to below 5 percent.
TABLE 2 different Ca2+Effect of concentration on Fusarium solani
| Ca2+Concentration of | Inhibition rate of Fusarium solani |
| 0.035 | 80％ |
| 0.04 | 89％ |
| 0.045 | 91％ |
| 0.05 | 94％ |
| 0.055 | 93％ |
| 0.060 | 88％ |
| 0.065 | 77％ |
TABLE 3 different Mg2+Effect of concentration on Fusarium solani
| Mg2+Concentration of | Inhibition rate of Fusarium solani |
| 0.025 | 80％ |
| 0.03 | 85％ |
| 0.035 | 91％ |
| 0.04 | 93％ |
| 0.045 | 90％ |
| 0.050 | 85％ |
| 0.055 | 77％ |
Example 3
Experiment in the field
The compound bactericide containing the perylenequinone compound comprises hypocrellin A, hypocrellin B and elsinochrome in a mass ratio of 9: 1.5: 3, 0.055mol/L Ca2+，0.035mol/L Mg2+0.06g/L of extract of ethyl acetate fraction of Shiraia bambusicola. The application method is that the compound bactericide is mixed and stirred evenly and then dissolved in water for dilution, the effective component content of the compound bactericide is 3 percent, the bactericide is directly sprayed on the common wheat field, various plant pathogenic bacteria in the wheat field can be effectively controlled, and the sterilization rate is controlled to be more than 98 percent.
Example 4
Experiment in the field
The compound bactericide containing the perylenequinone compound comprises hypocrellin A, hypocrellin B and elsinochrome in a mass ratio of 11: 2.3: 8.5, 0.049mol/LCa2+，0.042mol/LMg2+0.11g/L of extract of ethyl acetate fraction of Shiraia bambusicola. The application method is that the compound bactericide is mixed and stirred evenly and then dissolved in water for dilution, the effective component content of the compound bactericide is 2.8 percent, the bactericide is directly sprayed on the common wheat field, various plant pathogenic bacteria in the wheat field can be effectively controlled, and the sterilization rate is controlled to be more than 97 percent.
Example 5
Experiment in the field
Compound sterilization containing perylenequinone compoundThe preparation comprises hypocrellin A, hypocrellin B and elsinochrome in a mass ratio of 14: 3: 9, 0.045mol/LCa2+，0.045mol/L Mg2+0.14g/L of extract of ethyl acetate fraction of Shiraia bambusicola. The application method is that the compound bactericide is mixed and stirred evenly and then dissolved in water for dilution, the effective component content of the compound bactericide is 3.5 percent, the bactericide is directly sprayed on the common wheat field, various plant pathogenic bacteria in the wheat field can be effectively controlled, and the sterilization rate is controlled to be more than 96 percent.
In conclusion, the compound bactericide containing the perylenequinone compound has the best antibacterial activity and the variety of the added perylenequinone compound; the mass ratio of the perylenequinone compound; the kind of metal ion added; the concentration of the added metal ions; the type of the added tabasheer extract; the concentration of the added tabasheer extract has close relation. The formula of the compound bactericide containing the perylenequinone compound is as follows: the mass ratio of hypocrellin A, hypocrellin B and elsinochrome is (9-14): (1.5-3): (3-9), 0.045-0.055mol/LCa2+，0.035-0.045mol/LMg2+0.06-0.14g/L of extract of ethyl acetate fraction of Shiraia bambusicola. The compounded bactericide has obvious and excellent bacteriostatic effect.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (5)
1. A compound bactericide containing perylenequinone compounds is characterized in that: the active ingredients in the compound bactericide are hypocrellin A, hypocrellin B and elsinochrome A, wherein the mass ratio of the hypocrellin A to the hypocrellin B to the elsinochrome A is (9-14): (1.5-3): (3-9); the compound bactericide is also added with a synergist comprising metal ions Ca2+And Mg2+。
2. The compound bactericide as claimed in claim 1, wherein: metallic ion Ca2+The concentration is 0.045-0.055mol/L, and metal ion Mg2+The concentration is 0.035-0.045 mol/L.
3. The compound bactericide as claimed in claim 1, wherein: the synergist in the compound bactericide also comprises an extract of medicinal fungi of concretio silicea Bambusae seu Schizostachyi ethyl acetate.
4. The compound bactericide as claimed in claim 3, wherein: the concentration of the extract added with medicinal fungus of concretio silicea Bambusae seu Schizostachyi ethyl acetate is 0.06-0.14 g/L.
5. The compound bactericide as claimed in claim 4, wherein: the mass ratio of the active ingredients in the compound bactericide to hypocrellin A, hypocrellin B and elsinochrome A is (9-14): (1.5-3): (3-9) further comprising a metal ion Ca2+The concentration is 0.045-0.055mol/L, and metal ion Mg2+The concentration is 0.035-0.045mol/L, and the concentration of the extract of the medicinal fungus concretio silicea Bambusae seu Schizostachyi ethyl acetate part is 0.06-0.14 g/L.
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