CN114794152A - Pesticide composition containing metarhizium reineckii and application thereof - Google Patents

Pesticide composition containing metarhizium reineckii and application thereof Download PDF

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CN114794152A
CN114794152A CN202210243233.9A CN202210243233A CN114794152A CN 114794152 A CN114794152 A CN 114794152A CN 202210243233 A CN202210243233 A CN 202210243233A CN 114794152 A CN114794152 A CN 114794152A
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metarhizium
pesticide composition
reinhardtii
tetrachlorantraniliprole
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CN114794152B (en
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程东美
何俊烺
梁超鹏
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Zhongkai University of Agriculture and Engineering
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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Abstract

The invention discloses a pesticide composition containing metarhizium lepigone, which comprises a first component and a second component, wherein the first component is the metarhizium lepigone; the second component is tetrachlorantraniliprole or tebuconazole; the volume ratio of the number of conidia in the first component to the second component is as follows: 1X 10 6 ～5×10 11 CFU: 1 mL; wherein the mass concentration of the tetrachlorantraniliprole is 1-200 mg/L, and the mass concentration of the tetrazolium amide is 1-133.33 mg/L. The application provides a pesticide composition containing metarhizium reinhardtii and application thereof, and the synergistic action of the two components is utilizedThe use can effectively realize the control of spodoptera frugiperda larvae; and the use amount of chemical pesticides can be reduced, and the problems of environmental pollution and food safety are relieved.

Description

Pesticide composition containing metarhizium reineckii and application thereof
Technical Field
The invention relates to the field of pest control, and particularly relates to a pesticide composition containing metarhizium reinhardtii and application thereof.
Background
Spodoptera frugiperda, also known as fall armyworm, belongs to the genus Trichoplusia of the family Spodoptera, is a omnivorous pest native to the tropical and subtropical regions of the America, and is one of the world's important agricultural pests. The invasion of Spodoptera frugiperda is found for the first time in Jiangcheng county of Puer city in Yunnan China in 2019, 1 month and 11 months, and the Spodoptera frugiperda rapidly spreads to 26 major food producing areas in provinces and cities in one year and is in a continuous north moving trend. At present, the main measures for preventing and controlling spodoptera frugiperda are chemical prevention and control mainly, but serious environmental and food safety problems can be caused along with long-term dependence and abuse of chemical agents, harm is caused to people, livestock and other non-target organisms, meanwhile, the drug resistance of pests is increased, and the problems that the pests are difficult to prevent and control are caused. In order to relieve the problems caused by abuse of chemical pesticides to the maximum extent, the current green prevention and control technologies and means such as agricultural measures, biological prevention and control, physical prevention and control and the like are green prevention and control strategies for realizing sustainable prevention and control of spodoptera frugiperda. The entomopathogenic fungi have broad spectrum, can parasitize insects to cause insect death, can infect various insects, has the advantages of safety, environmental protection, long duration and the like, has wide application prospect, and is commonly beauveria bassiana and metarhizium anisopliae. However, the control speed is slow, the control effect is unstable and the like, so that the application range and popularization and application of the related pesticide are limited. In order to overcome the defects of the two parts and simultaneously exert the respective advantages and improve the control effect, the research on the compatibility of the fungi and the pesticide is started in the 60 th 20 th century, the biocontrol fungi and the low-dose chemical agent are combined, the use amount of the chemical pesticide can be reduced, the drug resistance of pests is delayed, the control duration is prolonged, and the stability of the pesticide is also enhanced.
Metarhizium anisopliae (Farl) Kepler, S A.Rehner & Humber, Nomuraea rileyi (Farl.) Samson, Ascomycota belonging to Ascomycota, Clavicipitaceae, is a entomogenous fungus with great biocontrol potential for Lepidoptera larvae, can cause pest epidemic under appropriate environmental conditions, and effectively control pests. Patent CN113025505A discloses a biological control method and application of Metarrhizium Laitense in Spodoptera frugiperda pupal stage, which only aims at the Spodoptera frugiperda pupal stage for control, and the applicable time node is narrow, so that effective protection in each time period cannot be realized.
Disclosure of Invention
The present invention is directed to solving, at least to some extent, one of the problems in the related art. Therefore, the invention aims to provide a pesticide composition containing metarhizium reinhardtii and application thereof, and the synergistic effect of the two components is utilized to effectively realize the control of spodoptera frugiperda larvae; and the use amount of chemical pesticides can be reduced, and the problems of environmental pollution and food safety are relieved.
In order to achieve the above object, the present invention provides a pesticide composition comprising metarhizium lepigone, comprising a first component and a second component, wherein the first component is metarhizium lepigone; the second component is tetrachlorantraniliprole or tebuconazole;
the volume ratio of the number of conidia in the first component to the second component is as follows: 1X 10 6 ～5×10 11 CFU: 1 mL; wherein the mass concentration of the tetrachlorantraniliprole is 1-200 mg/L, and the mass concentration of the tetrazolium amide is 1-133.33 mg/L.
Further, the first component is Metarrhizium limacinum in the form of a spore suspension, and the concentration of the spore suspension is 1 x 10 6 ～5×10 11 CFU/mL。
Further, the volume ratio of the first component to the second component is 1-10: 10-1.
Further, the spore suspension concentration of the green muscardine fungus is 1 × 10 8 CFU/mL, the mass concentration of the tetrachloro insect amide is 10-30mg/L, and the volume ratio of the first component to the second component is 1: 1.
Further, the volume ratio of the first component to the second component is 1-10: 10-1.
Further, the spore suspension concentration of the green muscardine fungus is 1 × 10 8 CFU/mL, the mass concentration of the tebuconazole is 10-30mg/L, and the volume ratio of the first component to the second component is 1: 1.
A method for preparing pesticide composition containing Metarrhizium Laevigatum comprises mixing first component and second component to obtain suspension; wherein the first component is Metarrhizium Laevigatum; the second component is tetrachlorantraniliprole or tebuconazole; the first componentThe volume ratio of the number of the conidia to the second component is as follows: 1X 10 6 ～5×10 11 CFU: 1 mL; wherein the mass concentration of the tetrachlorantraniliprole is 1-200 mg/L, and the mass concentration of the tetrazolium amide is 1-133.33 mg/L.
The application of a pesticide composition containing metarhizium reinhardtii is disclosed, and the pesticide composition is used for preventing and treating spodoptera frugiperda.
A method for controlling spodoptera frugiperda, wherein the pesticide composition is applied to a plant in the presence of insect pests.
Compared with the prior art, the technical scheme provided by the embodiment of the application has the following advantages: the mortality of the pesticide composition to spodoptera frugiperda 2-instar larvae can reach more than 80%, wherein the first composition and the second composition have obvious synergistic interaction, and compared with independent green muscardine lai, independent tetrachlorantraniliprole and independent tebuconazole amide, the pesticide composition has obvious control effect on spodoptera frugiperda larvae. The pesticide composition provided by the application reduces the using amount of the tetrachlorantraniliprole and the tebuconazole amide in practical application, can effectively relieve the problems of environment and food safety, and reduces the occurrence of drug resistance of pests.
Detailed Description
The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention. It is intended that all modifications or alterations to the methods, procedures or conditions of the present invention be made without departing from the spirit and substance of the invention. Unless otherwise specified, the experimental materials, reagents, instruments and the like used in the examples of the present invention are commercially available; unless otherwise specified, all technical means in the examples of the present invention are conventional means well known to those skilled in the art.
The pesticide composition comprises a first component and a second component, wherein the first component is metarhizium laevis ZHKUMR1 (the strain is preserved by Guangdong provincial microorganism culture collection center at 4-7 days 2020, and the preservation number is GDMCC 60995); the second component is tetrachlorantraniliprole or tebuconazole; first of allThe volume ratio of the number of conidia in the component to the second component is as follows: 1X 10 6 ～5×10 11 CFU: 1 mL; wherein the mass concentration of the tetrachlorantraniliprole is 1-200 mg/L, and the mass concentration of the tetrazolium amide is 1-133.33 mg/L. .
Preferably, the first component is Metarrhizium laevis ZHKUMR1 in the form of a spore suspension, and the concentration of the spore suspension is 1 × 10 6 ～5×10 11 CFU/mL。
Preferably, the volume ratio of the first component to the second component is 1-10: 10-1.
Preferably, the spore suspension concentration of Metarrhizium leydis ZHKUMR1 is 1 × 10 8 CFU/mL, the mass concentration of the tetrachloro insect amide is 10-30mg/L, and the volume ratio of the first component to the second component is 1: 1.
Preferably, the volume ratio of the first component to the second component is 1-10: 10-1.
Preferably, the spore suspension concentration of Metarrhizium laevis ZHKUMR1 is 1 × 10 8 CFU/mL, the mass concentration of the tebuconazole is 10-30mg/L, and the volume ratio of the first component to the second component is 1: 1.
The application provides a preparation method of a pesticide composition containing metarhizium lepigone, which specifically comprises the following steps: mixing the first component and the second component to prepare a suspension; wherein the first component is Metarrhizium laevis ZHKUMR 1; the second component is tetrachlorantraniliprole or tebuconazole; the volume ratio of the number of conidia in the first component to the second component is as follows: 1X 10 6 ～5×10 11 CFU: 1 mL; wherein the mass concentration of the tetrachlorantraniliprole is 1-200 mg/L, and the mass concentration of the tetrazolium amide is 1-133.33 mg/L. .
The application provides an application of a pesticide composition containing metarhizium lepigone, which is used for preventing and treating spodoptera frugiperda.
The pesticide composition is applied to plants when insect pests happen.
Tetrachlorantraniliprole is a dicarboxamide insecticide containing pyridyl pyrazole independently created by Shenyang chemical research institute, has similar chemical structure and action mechanism to chlorantraniliprole, belongs to o-formylaminobenzamide ryanodine receptor regulator, has control effect on various pests, has ultrahigh activity on lepidoptera, good quick action, long lasting period and low toxicity on mammals, and can be widely used for crops such as rice, corn, vegetables, fruit trees and the like.
Tetrazolamides (tetraniliprole) are novel broad spectrum insecticides of the anthranilamide type developed by bayer corporation; the pesticide is a new member in a family of diamide compounds such as chlorantraniliprole, cyantraniliprole and the like, and is a ryanodine receptor agonist; the tebuconazole amide can effectively control various lepidoptera, coleopteran and dipteran pests on rice, fruit trees, vegetables and other crops under low dosage.
The green muscardine fungus and the two insecticides are combined to form the unique pesticide composition, and the combination of the two components does not simply overlap the effects, but can play a synergistic role to achieve unexpected beneficial effects. The synergistic benefits of the two are illustrated by the specific examples below.
The 10% tetrachloro insect amide suspending agent used in the following examples is a 10% tetrachloro insect amide (9080 (TM)) suspending agent supplied by shenyang scientific chemicals, ltd. The 200g/L tebuconazole suspending agent is 200g/L tebuconazole suspending agent provided by Bayer company and having the model number of Vaygeo 200 SC.
Example 1
Effect of tetrachloro-worm amide on mycelial growth and spore germination of metarhizium laevis ZHKUMR 1:
the 10% tetrachloro-worm-amide suspending agent is diluted according to the dilution times shown in the table 1 to prepare tetrachloro-worm-amide suspending liquid with the effective concentrations of 200mg/L, 100mg/L, 50mg/L, 33.33mg/L and 25mg/L respectively.
The hypha growth measuring method comprises the following steps: the suspensions with different effective concentrations shown in Table 1 were added to SMAY quantitative culture medium at 50 deg.C, shaken, and poured into sterilized petri dishes with a diameter of 9cm to make drug-containing plates. Then, a mycelium block (D is 5mm) of a freshly cultured strain ZHKUMR1 was picked up and inoculated into the center of the drug-containing plate, the plate was covered with a dish cover, the plate was placed in a constant temperature incubator at 25. + -. 1 ℃ and L: D is 12h:12h, and the diameter of the colony was measured by the cross method on day 7. Treatment with each effective concentration of agent was repeated 3 times, using SMAY medium without pesticide as a control. The results of measuring the colony diameters are shown in Table 1, and the growth inhibition ratio is calculated by the following formula:
Figure BDA0003543640550000061
spore germination test method: adding the suspensions with different effective concentrations shown in the table 1 into the green muscardine fungus spore suspension respectively, fully shaking uniformly to dilute the reagents with different effective concentrations until the spore amount is 80-100 in the visual field under a microscope, dripping 20 mu L of the drug-containing spore suspension on a concave glass sheet by using a liquid transfer gun, and uniformly coating. Setting 4 replicates for each concentration, calculating an average value, taking sterile water as a reference, carrying out moisture preservation culture in an incubator at 25 ℃ for 24 hours, then carrying out microscopic examination, and calculating the total number and the germination number of spores, wherein the spore germination inhibition rate is calculated by adopting the following formula:
Figure BDA0003543640550000062
the results are shown in Table 1.
TABLE 1 Effect of Tetrachlorantraniliprole on the growth of the hyphae of Metarrhizium leylanicum ZHKUMR1
Figure BDA0003543640550000063
And (4) conclusion: the influence of the tetrachlorantraniliprole on the growth of the metarhizium lepigy bacterial colony shows that the tetrachlorantraniliprole has negative inhibition rate on the growth of the metarhizium lepigy bacterial colony under different concentration levels, and the tetrachlorantraniliprole has promotion effect on the growth of the metarhizium lepigy hyphae.
The tetrachloro-worm amide has strong inhibiting effect at high concentration, and the influence of low concentration on spore germination is small. From the results of the experiment in example 1, it is found that tetrachlorantraniliprole has a certain compatibility with spores under the recommended concentration condition and can be used as an active ingredient of the pesticidal composition of the present invention.
Example 2
Effect of tetrazolium amide on mycelial growth of metarhizium leylanicum ZHKUMR 1:
the test method comprises the following steps: diluting 200g/L of tebuconazole suspending agent according to the dilution times shown in Table 2 to prepare tebuconazole suspensions with effective concentrations of 133.33mg/L, 66.67mg/L, 44.44mg/L, 33.33mg/L and 26.67mg/L respectively;
the hypha growth measuring method comprises the following steps: the suspensions with different effective concentrations shown in Table 2 were added to SMAY quantitative culture medium at 50 deg.C, shaken, and poured into sterilized petri dishes with a diameter of 9cm to make drug-containing plates. Then, a mycelium block (D is 5mm) of a freshly cultured strain ZHKUMR1 was picked up and inoculated into the center of the drug-containing plate, the plate was covered with a dish cover, the plate was placed in a constant temperature incubator at 25. + -. 1 ℃ and L: D is 12h:12h, and the diameter of the colony was measured by the cross method on day 7. Treatment with each effective concentration of agent was repeated 3 times, using SMAY medium without pesticide as a control. The results of the colony diameters measured are shown in table 2; the growth inhibition rate is calculated by the following formula:
Figure BDA0003543640550000071
spore germination test method: adding the suspension liquid with different effective concentrations shown in the table 2 into the green muscardine fungus spore suspension liquid respectively, fully shaking uniformly to dilute the reagents with different effective concentrations to 80-100 spores under a microscope, dripping 20 mu L of the drug-containing spore suspension liquid on a concave glass sheet by using a liquid transfer gun, and uniformly coating. Setting 4 replicates for each concentration, calculating an average value, taking sterile water as a reference, carrying out moisture preservation culture in an incubator at 25 ℃ for 24 hours, then carrying out microscopic examination, and calculating the total number and the germination number of spores, wherein the spore germination inhibition rate is calculated by adopting the following formula:
Figure BDA0003543640550000081
the results are shown in Table 2.
TABLE 2 Effect of Tetratolfenpyrad on the growth of the hyphae of Metarhizium limeri ZHKUMR1
Figure BDA0003543640550000082
And (4) conclusion: the influence of the tetrazolium amide on the growth of the metarhizium lepigone colonies shows that the tetrazolium amide has negative inhibition rate on the growth of the metarhizium lepigone colonies under different concentration levels, and the tetrazolium amide has promotion effect on the growth of the metarhizium lepigone mycelia.
The influence of the concentrations of the tetrazolium amide on the spore germination of the metarhizium laevis ZHKUMR1 is not obviously different, and the inhibition rate is lower. The experimental results of the above example 2 show that the tetrazolium amide has good compatibility with the spores of the metarhizium reinhardtii ZHKUMR1, and can be used as the active ingredient of the pesticide composition.
Example 3
The combined toxicity of compounding the metarhizium leylanicum ZHKUMR1 with the tetrachlorantraniliprole and the tebuconazole to spodoptera frugiperda:
the test method comprises the following steps: 10% of tetrachlorantraniliprole suspending agent and 200g/L of tebuconazole suspending agent are respectively mixed with the concentration of 1 multiplied by 10 8 CFU/mL metarhizium anisopliae spore suspension is mixed in a volume ratio of 1:1, and the mixed solution is diluted to pesticide compositions with serial concentrations by using the spore suspension with equal concentration. Sterile water was used as a control group.
The activity test adopts an insect soaking method, healthy 2-3-instar spodoptera frugiperda larvae are respectively soaked in pesticide compositions with different concentrations for 6s, then the larvae are taken out and aired, then the treated larvae are placed in a 6-hole insect raising plate for normal raising, and the number of dead insects is investigated day by day and recorded. Meanwhile, the treatment with the Metarrhizium laevis ZHKUMR1, the tetrachloro-zoon amide and the tetrazolium amide is singly adopted. Each concentration was repeated 3 times, with 50 test insects per repetition. The calculation formula for experimental data processing is as follows:
Figure BDA0003543640550000091
Figure BDA0003543640550000092
mix theoretical mortality (%) ═ 1- (1-single a mortality) × (1-single B mortality); wherein, the single agent A refers to the pesticide composition only containing the tetrachlorantraniliprole, and the single agent B refers to the pesticide composition only containing the tebuconazole.
Figure BDA0003543640550000093
Statistical analysis was performed using SPSS26.0 data processing software to calculate mortality, LC for each pesticide composition 50 The toxicity regression equation, the chi-square value and the Duncan's new range test are adopted to carry out difference significance analysis so as to evaluate the activity of the pesticide composition.
In a synergistic virulence test, based on the single use of the agent LC 50 Preparing liquid medicine, treating 50 head worms each for 3 times, observing and counting death numbers after 3 days of treatment, and calculating the synergistic virulence index. The results of the experiments are shown in tables 3 and 4.
TABLE 3 virulence of Spodoptera frugiperda larvae in combination with different pesticidal compositions and Metarhizium laevis ZHKUMR1 (3 d after treatment)
Figure BDA0003543640550000101
The concentration is 1.0X 10 8 cfu/mL Metarrhizium lygorii is respectively mixed with tetrachlorantraniliprole and tebuconazole, and mixed with liquid medicine to resist LC (LC) of spodoptera frugiperda larvae 50 Are respectively 12.19mg/L and 9.26 mg/L. LC of single agent of tetrachlorantraniliprole and tebuconazole 50 16.70mg/L and 23.04mg/L respectively, LC of mixture 50 Is obviously lower than LC of tetrachlorantraniliprole and tebuconazole 50 And the content of the pesticide is reduced by 26.98 percent and 59.80 percent respectively, so that the dosage of the pesticide can be effectively reduced after the green muscardine fungus and the pesticide are mixed. The significance of the four treatments is more than 0And 05, showing that the established model has better fitting with the actual data.
TABLE 4 synergistic Effect of Tetrachlorantraniliprole and Tetratolanilide in combination with Metarhizium laevis ZHKUMR1 on Spodoptera frugiperda larvae
Figure BDA0003543640550000102
It is worth mentioning that: the data in the table are statistics of observations 3d after treatment. Wherein the concentration of the single agent of the green muscardine fungus is 1 × 10 8 CFU/mL, 10% of the chlorantraniliprole suspending agent and 200g/L of the tetrazolium amide suspending agent are respectively diluted 6000 times and 10000 times, specifically 16.67mg/L of the chlorantraniliprole and 20mg/L of the tetrazolium amide. When c.i is not less than 20, it is judged as synergistic effect, when c.i is not more than-20, it is judged as antagonistic effect, and when-20<c.i<And an additive action is determined at 20.
The synergistic effect result shows that the lethal effect of the mixed preparation on the spodoptera frugiperda larvae of 2-3 years is good, the corrected mortality rate of the mixed preparation is 82.76% and 86.21% respectively, the synergistic index of the mixed preparation is 31.47 and 34.24 respectively and is more than 20, the synergistic effect is shown, the lethal rate of the mixed preparation of the metarhizium anisopliae and the tetrazolium amide on the spodoptera frugiperda larvae is up to 86.67%, and the control effect is good.
In summary, the mortality rate of the pesticide composition to spodoptera frugiperda 2 instar larvae can reach more than 80%, wherein the first composition and the second composition have obvious synergistic interaction effect, and compared with independent green muscardine lai, independent tetrachlorantraniliprole and independent tebuconazole amide, the pesticide composition has obvious control effect on spodoptera frugiperda larvae. The pesticide composition provided by the application reduces the using amount of the tetrachlorantraniliprole and the tebuconazole amide in practical application, can effectively relieve the problems of environment and food safety, and reduces the occurrence of drug resistance of pests.
It is to be understood that the foregoing examples, while indicating the preferred embodiments of the invention, are given by way of illustration and description, and are not to be construed as limiting the scope of the invention; it should be noted that, for those skilled in the art, the above technical features can be freely combined, and several changes and modifications can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention; therefore, all equivalent changes and modifications made within the scope of the claims of the present invention should be covered by the claims of the present invention.

Claims (9)

1. A pesticide composition containing metarhizium reinhardtii, which is characterized by comprising a first component and a second component, wherein the first component is the metarhizium reinhardtii; the second component is tetrachlorantraniliprole or tebuconazole;
the volume ratio of the number of conidia in the first component to the second component is as follows: 1X 10 6 ～5×10 11 CFU: 1 mL; wherein the mass concentration of the tetrachlorantraniliprole is 1-200 mg/L, and the mass concentration of the tetrazolium amide is 1-133.33 mg/L.
2. The pesticidal composition comprising metarhizium reinhardtii according to claim 1, wherein the first component is metarhizium reinhardtii in the form of a spore suspension, and the concentration of the spore suspension is 1 x 10 6 ～5×10 11 CFU/mL。
3. The pesticide composition containing metarhizium reinhardtii as claimed in claim 2, wherein the volume ratio of the first component to the second component is 1-10: 10-1.
4. The pesticide composition containing metarhizium reinhardtii as claimed in claim 3, wherein the concentration of spore suspension of metarhizium reinhardtii is 1 x 10 8 CFU/mL, the mass concentration of the tetrachloro insect amide is 10-30mg/L, and the volume ratio of the first component to the second component is 1: 1.
5. The pesticide composition containing metarhizium reinhardtii as claimed in claim 2, wherein the volume ratio of the first component to the second component is 1-10: 10-1.
6. The pesticide composition containing metarhizium reinhardtii as claimed in claim 5, wherein the concentration of spore suspension of metarhizium reinhardtii is 1 x 10 8 CFU/mL, the mass concentration of the tebuconazole is 10-30mg/L, and the volume ratio of the first component to the second component is 1: 1.
7. A preparation method of a pesticide composition containing Metarrhizium Laevigatum is characterized in that a first component and a second component are mixed to prepare a suspension; wherein the first component is Metarrhizium Laevigatum; the second component is tetrachlorantraniliprole or tebuconazole; the volume ratio of the number of conidia in the first component to the second component is as follows: 1X 10 6 ～5×10 11 CFU: 1 mL; wherein the mass concentration of the tetrachlorantraniliprole is 1-200 mg/L, and the mass concentration of the tetrazolium amide is 1-133.33 mg/L.
8. Use of a pesticidal composition comprising metarhizium reinhardtii for the control of spodoptera frugiperda according to any of claims 1 to 6.
9. A method for controlling Spodoptera frugiperda, characterized in that a pesticide composition according to any one of claims 1 to 6 is applied to the plants at the time of insect infestation.
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Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110669676A (en) * 2019-10-25 2020-01-10 云南农业大学 Metarrhizium leydii MRJCBT190808 and application thereof in control of spodoptera frugiperda
CN112314630A (en) * 2020-11-30 2021-02-05 安徽丰乐农化有限责任公司 Insecticidal composition for preventing and treating cabbage loopers

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110669676A (en) * 2019-10-25 2020-01-10 云南农业大学 Metarrhizium leydii MRJCBT190808 and application thereof in control of spodoptera frugiperda
CN112314630A (en) * 2020-11-30 2021-02-05 安徽丰乐农化有限责任公司 Insecticidal composition for preventing and treating cabbage loopers

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