CN116114712B - Bactericidal composition containing bacillus amyloliquefaciens and application thereof - Google Patents
Bactericidal composition containing bacillus amyloliquefaciens and application thereof Download PDFInfo
- Publication number
- CN116114712B CN116114712B CN202211490906.7A CN202211490906A CN116114712B CN 116114712 B CN116114712 B CN 116114712B CN 202211490906 A CN202211490906 A CN 202211490906A CN 116114712 B CN116114712 B CN 116114712B
- Authority
- CN
- China
- Prior art keywords
- bacillus amyloliquefaciens
- whig
- bactericidal composition
- disease
- control
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/56—1,2-Diazoles; Hydrogenated 1,2-diazoles
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention belongs to the field of agricultural bactericides, and particularly relates to a bactericidal composition containing bacillus amyloliquefaciens, which is characterized by comprising a bacillus amyloliquefaciens WHIG microbial inoculum and fluxapyroxad hydroxylamine; the mass ratio of the bacillus amyloliquefaciens WHIG microbial inoculum to the fluxapyroxad hydroxylamine is 100:1-1:10. The invention utilizes the control strategy of combining biological control and chemical control, not only can reduce the application amount of chemical agents and improve the stability of biological control, but also can achieve the effect of effectively controlling diseases, and the composition shows remarkable synergy.
Description
Technical Field
The invention belongs to the field of agricultural bactericides, and particularly relates to a bactericidal composition containing bacillus amyloliquefaciens and application thereof.
Background
Rape belonging to the phylum of the angiospermAngiospermae) Dicotyledon classDicotyledons) Dillenia indica subclassDilleniidae) The order of the white cauliflowerBrassicales) The cruciferae is @ aBrassicaceae) The brassica isBrassica). The rape has economic value and ornamental value, can be used for ornamental during flowering, can be squeezed into rape seed for eating after the rape flower withers, and has high medicinal value and industrial value. Currently, chinese canola plant area is the second most worldwide, only after india. The main planting places of rape in China are Anhui, sichuan, jiangxi, jiangsu and Henan.
Rape is the most important oil crop in China, is one of four oil crops in the world, has 33% -50% of oil content in seeds, has important economic value, and is widely applied to medicine, food, industrial raw materials and the like. The rape has long growth period and various diseases. Among them, sclerotinia is the most serious. During the growing period of rape, sclerotinia rot of rape can occur, and the disease occurrence is the highest in flowering and fruiting period. The germs can infect all parts on the ground of the rape plant, especially the loss caused by the stalk is the largest after the disease is developed. Most of germs are infected from the residual flowers which are opened and aborted, the germs are in a water stain shape or a pale shape at first, and then petals fall to cause the disease of stems and leaves. After the stem part of the plant is infected with sclerotium, the stem part of the plant is initially presented as a water stain-like disease spot, and then the disease spot gradually turns brown, is recessed inwards, and white flocculent hypha and black mouse manure-like sclerotium grow out, and when the air becomes dry, the disease part of the stem base can dry, shrink and die. Plant leaves are infected with sclerotinia and initially mainly show that the lesions are in dark cyan water stains and irregular, and concentric circles appear. When the air becomes moist, the growth rate of the lesion becomes fast, causing rapid decay of the leaf, and then white flocculent hyphae and black mouse-dung-like sclerotium grow on the lesion. Typical symptoms of sclerotium disease are white flocculent mold and black mouse fecal sclerotium grown by the disease department.
For a long time, prevention and treatment of sclerotinia rot of colza mainly depends on chemical prevention and treatment. The bactericide has a certain prevention and control effect on the sclerotium disease of rape, but the action effect is influenced by various factors such as the application time, the plant development stage, the environmental conditions and the like, and a large amount of chemical agents can accumulate chemical pesticide residues to influence the health of human bodies, and the ecological balance can be destroyed to cause environmental pollution. The long-term use of chemical agents can lead to drug resistance of pathogenic bacteria, and lead to reduced or even failed control effects, which forces people to seek safer and more effective control ways. Rape is used as a worldwide oil crop, has wide application in food, medicine and other aspects, and the use of biocontrol bacteria for disease control can reduce the use of chemical pesticides and reduce the pollution to the environment, and is increasingly favored by people as an environment-friendly control technology.
Gram positive buds of bacillus amyloliquefaciensBacillus is a bacterium with high relativity with bacillus subtilis and is widely distributed in nature. The bacillus amyloliquefaciens can produce a series of metabolites with the activity of widely inhibiting fungi and bacteria in the growth process, has wide application prospect in the aspect of biological control, and gradually becomes a microorganism with the development potential of biological pesticides. Bacillus amyloliquefaciens WHIG is obtained by separating rice disease control subject components from the institute of plant protection and agricultural product quality safety of Anhui province, and is preserved in China general microbiological culture Collection center (CGMCC) with a preservation number of 10722 at 15/04/2015. The strain is named WHIG, and the strain is named in a classification mode: bacillus amyloliquefaciens @Bacillusamyloliquefaciens) (refer to CN107136120 a).
The fluxapyroxad hydroxylamine is a novel pyrazole amide fungicide, belongs to succinic acid dehydrogenase inhibitor (SDHI) fungicide, is a pyrazole-4-amide compound, and has the advantages of novel structure, high activity and broad fungicide spectrum; however, the current production and use costs of fluxapyroxad hydroxylamine are high, and there is a great risk of resistance when used alone for a long period of time.
The control strategy combining biological control and chemical control is utilized, so that the application amount of chemical agents can be reduced, the stability of biological control can be improved, the effect of effectively controlling diseases can be achieved, and no report on the combined use of bacillus amyloliquefaciens WHIG and fluxapyroxad is available in the prior art. The applicant researches find that the combination of bacillus amyloliquefaciens WHIG and fluxapyroxad hydroxylamine has a good synergistic effect on sclerotinia rot of colza.
Disclosure of Invention
The invention utilizes the control strategy of combining biological control and chemical control, not only can reduce the application amount of chemical agents, but also can improve the stability of biological control, and the composition shows remarkable synergy.
The technical scheme of the invention is as follows:
a bactericidal composition containing bacillus amyloliquefaciens, which is characterized by comprising bacillus amyloliquefaciens WHIG bacterial agent and fluxapyroxad hydroxylamine; the bacillus amyloliquefaciens WHIG microbial inoculumAnd the mass ratio of the fluxapyroxad hydroxylamine is 100:1-1:10; the bacillus amyloliquefaciens WHIG is preserved in China general microbiological culture collection center (CGMCC No. 10722); bacillus amyloliquefaciens WHIG microbial inoculum concentration 1×10 6 -1×10 10 cfu／mL。
Preferably, the concentration of the bacillus amyloliquefaciens WHIG microbial inoculum is 1 multiplied by 10 7 -1×10 9 cfu/mL; the mass ratio of the bacillus amyloliquefaciens WHIG microbial inoculum to the fluxapyroxad hydroxylamine is 80:1-10:1. Further preferably, the concentration of the bacillus amyloliquefaciens WHIG microbial inoculum is 1×10 8 cfu/mL; the mass ratio of the bacillus amyloliquefaciens WHIG microbial inoculum to the fluxapyroxad hydroxylamine is 80:1-10:1.
The bactericidal composition also comprises pesticide auxiliary ingredients, and is prepared into a pesticide preparation. The pesticide preparation is suspending agent, wettable powder and water dispersible granule. The auxiliary materials are prepared by adopting a conventional preparation method by selecting conventional auxiliary material components in the field.
The bactericidal composition can be used for preventing and controlling crop diseases. Preferably the crop disease is sclerotinia rot of colza.
By adopting the technical scheme, the invention has the following beneficial effects:
the invention utilizes the control strategy of combining biological control and chemical control, not only can reduce the application amount of chemical agents and improve the stability of biological control, but also can achieve the effect of effectively controlling diseases.
When the mass ratio of WH1G bacterial suspension to fluxapyroxad hydroxylamine is 80:1-10:1, the composition shows a synergistic effect on sclerotinia sclerotiorum, so that the dosage of single dosage can be effectively reduced, and the generation of drug resistance of pathogenic bacteria can be delayed.
Detailed Description
The invention is further illustrated with reference to specific embodiments.
Example 1: indoor toxicity test of compound medicament on sclerotinia rot of colza
1.1 test materials
Biocontrol bacteria: bacillus amyloliquefaciens WH1G, anhui province agricultureThe institute of plant protection and agricultural product quality safety. Culturing in modified NA culture medium (beef extract 1.0g, peptone 5.0g, yeast extract 5.0g, naCl5.0g, sucrose 10.0g, agar 20.0g, distilled water 1000mL, pH 6.8-7.0) at 28deg.C. Preparing Bacillus amyloliquefaciens WH1G bacterial suspension with concentration of 1×10 8 cfu／mL。
Sclerotinia sclerotiorum of rapeSclerotinia sclerotiorum) Separated, screened, identified and stored by the institute of plant protection and agricultural product quality safety of the national academy of agricultural science of Anhui province.
Fluoxazoyl hydroxylamine, commercially available.
1.2 test methods
The hypha growth rate method is adopted. On the basis of pre-experiments, PDA plates containing medicaments with different gradient concentrations are prepared, sclerotinia sclerotiorum bacterial blocks (with the diameter of 5 mm) are placed in the center of the medicament-containing plates, and the plates are placed in a 28 ℃ incubator for culture. And controls were set, and each treatment was repeated 4 times. When the control colony grows to be 2/3 of the culture dish, measuring the diameter of each treated colony by adopting a crisscross method, and calculating the average inhibition rate.
Average inhibition ratio = [ (average control colony diameter-average treatment colony diameter)/(average control colony diameter-5.0) ]×100%.
Converting the hypha growth inhibition rate into inhibition probability value (y), converting the concentration of the medicament into concentration logarithm (x), taking a toxicity regression line according to the concentration logarithm as an abscissa and the probability value as an ordinate, solving a toxicity regression equation of single medicament and a mixed medicament thereof on bacteria, and calculating EC 50 Values and correlation coefficient r values.
According to the grand Yunpei method, the toxicity index of each medicament and the co-toxicity coefficient (CTC value) of the mixture are calculated, namely CTC is less than or equal to 80 and is antagonistic, CTC is 80 and CTC is 120 and is additive, and CTC is more than or equal to 120 and is synergistic.
Co-toxicity coefficient (CTC) =measured toxicity index of the mix (ATT)/theoretical toxicity index of the mix (TTI).
Measured virulence index (ATI) =standard agent EC 50 Test agent EC 50 ×100
Theoretical Toxicity Index (TTI) =a agent toxicity index x percentage of agent toxicity index in mixture + B agent toxicity index x percentage of agent toxicity in mixture.
1.3 test results
As is clear from Table 1, the EC of WH1G bacterial suspension against Sclerotinia sclerotiorum 50 A value of 20.68mg/L; EC of fluxapyroxad hydroxylamine against sclerotinia sclerotiorum 50 The value was 0.19mg/L. When the mass ratio of WH1G bacterial suspension to fluxapyroxad hydroxylamine is 80:1-10:1, the suspension and the fluxapyroxad hydroxylamine show synergistic effect on sclerotinia sclerotiorum, and the suspension and the fluxapyroxad hydroxylamine show additive effect under other ratio conditions. When the composition shows synergistic effect, the dosage of single agent can be effectively reduced, and the occurrence of disease drug resistance can be slowed down.
Table 1 indoor toxicity test of formulated medicament against sclerotinia rot of colza
Remarks: 1mL in the bacterial suspension configuration was calculated as 1 g.
Example 2: potted plant test of compound medicament on sclerotinia rot of colza
2.1 test materials
Biocontrol bacteria: bacillus amyloliquefaciens WH1G, institute of plant protection and agricultural product quality safety at the national academy of agricultural sciences, anhui province. Culturing in modified NA culture medium (beef extract 1.0g, peptone 5.0g, yeast extract 5.0g, naCl5.0g, sucrose 10.0g, agar 20.0g, distilled water 1000mL, pH 6.8-7.0) at 28deg.C. Preparing Bacillus amyloliquefaciens WH1G bacterial suspension with bacterial content of 1×10 8 cfu／mL。
Sclerotinia sclerotiorum of rapeS.sclerotiorum) Separated, screened, identified and stored by the institute of plant protection and agricultural product quality safety of the national academy of agricultural science of Anhui province.
Fluoxazoyl hydroxylamine, commercially available.
2.2 test methods
The medicines with different concentrations are respectively and evenly sprayed on the surfaces of rape leaves, and after 24 hours, sclerotinia sclerotiorum bacterial blocks are inoculated on the surfaces of the rape leaves. A control was set. The test was repeated 3 times for 10 strains per treatment. Placing in an incubator for wet culture. Disease severity was investigated and disease index calculated after 7d inoculation, respectively.
Disease severity grading criteria:
grade 0, no disease;
stage 1, the disease area is less than 5% of the whole leaf area;
stage 3, wherein the disease area accounts for 5% -10% of the total leaf area;
grade 5, the disease area accounts for 11% -30% of the total leaf area;
grade 7, the disease area accounts for 31% -50% of the whole leaf area;
grade 9, the disease area is more than 50% of the whole leaf area.
Disease index = Σ [ (number of disease plants at each stage×number of relative disease stages)/(total number of investigation×number of highest disease stages) ]100
Control effect (%) = [ (control disease index-treatment disease index)/control disease index ] ×100
2.3 test results
As shown in table 1, the control effect of the WH1G bacterial suspension alone on sclerotinia rot of colza is 50.9%; the prevention and treatment effect of the fluoxastrobin alone on the sclerotinia rot of colza is 59.4 percent. After the WH1G bacterial suspension and the fluxapyroxad hydroxylamine are combined, the prevention and treatment effect of the medicament on the sclerotinia rot of colza can be obviously improved, and the synergistic effect is obvious.
Table 2 potted plant test of compound preparation for sclerotinia rot of colza
Remarks: 1mL in the bacterial suspension configuration was calculated as 1 g.
Finally, it should also be noted that the above list is merely a few specific embodiments of the present invention. Obviously, the invention is not limited to the above embodiments, but many variations are possible. All modifications which can be directly derived or suggested by the person skilled in the art from the teaching of the present disclosure are considered to be within the scope of the present invention.
Claims (4)
1. A bactericidal composition containing bacillus amyloliquefaciens, which is characterized by comprising bacillus amyloliquefaciens WHIG bacterial agent and fluxapyroxad hydroxylamine; the mass ratio of the bacillus amyloliquefaciens WHIG microbial inoculum to the fluxapyroxad hydroxylamine is 80:1-10:1;
the bacillus amyloliquefaciens WHIG is preserved in China general microbiological culture collection center (CGMCC No. 10722);
bacillus amyloliquefaciens WHIG microbial inoculum concentration 1×10 8 cfu／mL。
2. The bactericidal composition of claim 1, further comprising a pesticidal adjuvant component, wherein the bactericidal composition is formulated as a pesticidal formulation.
3. The bactericidal composition of claim 2, wherein the pesticide formulation is a suspension, a wettable powder, or a water dispersible granule.
4. Use of a fungicidal composition according to any one of claims 1-3 for controlling crop diseases, wherein the crop disease is sclerotium disease of colza.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211490906.7A CN116114712B (en) | 2022-11-25 | 2022-11-25 | Bactericidal composition containing bacillus amyloliquefaciens and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211490906.7A CN116114712B (en) | 2022-11-25 | 2022-11-25 | Bactericidal composition containing bacillus amyloliquefaciens and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN116114712A CN116114712A (en) | 2023-05-16 |
| CN116114712B true CN116114712B (en) | 2023-08-11 |
Family
ID=86298184
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211490906.7A Active CN116114712B (en) | 2022-11-25 | 2022-11-25 | Bactericidal composition containing bacillus amyloliquefaciens and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116114712B (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104507314A (en) * | 2012-01-25 | 2015-04-08 | 拜耳知识产权有限责任公司 | Active compounds combination containing fluopyram bacillus and biologically control agent |
| CN107136120A (en) * | 2017-05-19 | 2017-09-08 | 安徽省农业科学院植物保护与农产品质量安全研究所 | A kind of bactericidal composition containing bacillus amyloliquefaciens and Boscalid |
| CN107736379A (en) * | 2017-11-01 | 2018-02-27 | 广州市林业和园林科学研究院 | Application of the bacillus amyloliquefaciens in fungal diseases of plants is prevented and treated |
| CN109362727A (en) * | 2018-12-13 | 2019-02-22 | 安阳工学院 | A kind of wheat scab Biological agents and preparation method thereof |
| CN111040976A (en) * | 2020-01-06 | 2020-04-21 | 安徽农业大学 | Bacillus amyloliquefaciens and application thereof |
| CN111741681A (en) * | 2018-02-23 | 2020-10-02 | 日本曹达株式会社 | Agricultural and horticultural bactericide composition |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017018464A1 (en) * | 2015-07-27 | 2017-02-02 | 住友化学株式会社 | Plant disease control composition and plant disease control method |
| BR102018010543A2 (en) * | 2018-05-23 | 2019-12-10 | Agrivalle Brasil Ind E Comercio De Produtos Agricolas Ltda | composition for biological control of phytopathogenic fungi |
-
2022
- 2022-11-25 CN CN202211490906.7A patent/CN116114712B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104507314A (en) * | 2012-01-25 | 2015-04-08 | 拜耳知识产权有限责任公司 | Active compounds combination containing fluopyram bacillus and biologically control agent |
| CN107136120A (en) * | 2017-05-19 | 2017-09-08 | 安徽省农业科学院植物保护与农产品质量安全研究所 | A kind of bactericidal composition containing bacillus amyloliquefaciens and Boscalid |
| CN107736379A (en) * | 2017-11-01 | 2018-02-27 | 广州市林业和园林科学研究院 | Application of the bacillus amyloliquefaciens in fungal diseases of plants is prevented and treated |
| CN111741681A (en) * | 2018-02-23 | 2020-10-02 | 日本曹达株式会社 | Agricultural and horticultural bactericide composition |
| CN109362727A (en) * | 2018-12-13 | 2019-02-22 | 安阳工学院 | A kind of wheat scab Biological agents and preparation method thereof |
| CN111040976A (en) * | 2020-01-06 | 2020-04-21 | 安徽农业大学 | Bacillus amyloliquefaciens and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 解淀粉芽孢杆菌WH1G与氟啶胺协同防治草莓灰霉病;谷春艳 等;农药(第12期);第77-81页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN116114712A (en) | 2023-05-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP2000513582A (en) | Antimicrobial microbial preparation, its production method and treatment method | |
| CN110713951B (en) | Pantoea agglomerans strain and application thereof | |
| CN109169712B (en) | Composite biological control agent and preparation method and application thereof | |
| CN109182219B (en) | Bacillus mojavensis promoting growth of clostridium sargassum and application thereof | |
| CN116114712B (en) | Bactericidal composition containing bacillus amyloliquefaciens and application thereof | |
| JP2003531603A (en) | Microbial preparation for biological control using novel Trichoderma microorganism strain and method for producing the same | |
| CN112772658A (en) | Bactericidal composition of spirosporium roseum spores and boscalid and application of bactericidal composition | |
| CN106967656B (en) | Bacillus licheniformis and application thereof | |
| CN116267995B (en) | Sterilization composition for preventing and treating wheat scab and application thereof | |
| CN110720470A (en) | Application of bacillus thuringiensis in prevention and treatment of rootworm insects | |
| CN113383797B (en) | Tetramycin-containing bactericidal composition | |
| CN114058542B (en) | Paenibacillus polymyxa microbial inoculum and control effect thereof on carrot root rot | |
| Devaki et al. | Comparative efficacy of native Bacillus thuringiensis strains in two different sprayable formulations against Spodoptera litura in groundnut | |
| KR20150079323A (en) | Composition comprising Pseudomonas otitidis strain YJR27 for controlling plant diseases and plant-growth promiting effect | |
| CN114128718B (en) | Pharmaceutical composition for preventing and treating sclerotinia rot of sunflower | |
| CN116019116B (en) | Pesticide powder and application method thereof | |
| KR102444742B1 (en) | Composition for relieving brown spot disease of crops | |
| CN110862948B (en) | Bacillus amyloliquefaciens and application thereof in preventing and controlling false smut of rice | |
| CN116240130B (en) | Acid-resistant darkness single spore bacterium TXB1-10 and application thereof | |
| CN109619098B (en) | Rice antibacterial agent and application thereof | |
| CN112226387B (en) | White bacillus and application thereof in preventing and treating basal rot of corn stalk | |
| CN115044493B (en) | Nematode biocontrol bacterial strain and application thereof in preventing and controlling tobacco root knot nematode disease | |
| CN115948301B (en) | Oligosaccharin composite microbial inoculant for preventing and controlling banana vascular wilt and application thereof | |
| CN110352986B (en) | Composition containing bacillus subtilis and prochloraz and application thereof | |
| CN117882745A (en) | Bactericidal composition for preventing and treating root rot of dwarf lilyturf tuber and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |