CN104604947B - One kind kills planthopper Metarhizium anisopliae preparation and its application - Google Patents
One kind kills planthopper Metarhizium anisopliae preparation and its application Download PDFInfo
- Publication number
- CN104604947B CN104604947B CN201510084106.9A CN201510084106A CN104604947B CN 104604947 B CN104604947 B CN 104604947B CN 201510084106 A CN201510084106 A CN 201510084106A CN 104604947 B CN104604947 B CN 104604947B
- Authority
- CN
- China
- Prior art keywords
- metarhizium anisopliae
- planthopper
- cqma421
- preparation
- hel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
Application of the Metarhizium anisopliae CQMa421 bacterial strains in the medicament for preparing preventing and treating planthopper.The medicament of preventing and treating planthopper is made up of green muscardine fungus CQMa421 xerospores powder 35 52%, diatomite 40 50%, nano-silicon 1 2%, sodium carboxymethylcellulose 3 5%, O 30 2 4% and HEL 40 2 4%, with percentage by weight.Metarhizium anisopliae CQMa421 bacterial strains of the present invention can infect planthopper, and fatal rate can reach 32.6%, strengthen Metarhizium anisopliae CQMa421 bacterial strains made from the method for virulence using the present invention, its fatal rate can reach 98.7 100%, LT50Shorten 57.4 61.4%, effect is very prominent.Effective cooperation of each composition in Metarhizium anisopliae preparation of the present invention, serve synergistic function, spraying is watered using Metarhizium anisopliae preparation of the present invention, more than 80% is reached to the field efficacy of planthopper, said preparation has good uvioresistant and wetting and dispersing characteristic, and the bioactivity in long-term storage is maintained, period of storage is up to 12 months.
Description
Technical field
The present invention relates to a kind of new opplication of Metarhizium anisopliae bacterial strain, and in particular to one kind kills the green deadlock of planthopper chafer
Bacteria preparation and its application, belong to field of biological pesticide.
Technical background
Planthopper is the main migratory pest of rice, and the country is distributed widely in the main rice region in the whole nation, and serious threatens
China's grain-production.At present, broad spectrum activity chemical pesticide control planthopper is mainly applied, not only directly affects health and aquatic products
The safety of aquaculture, and largely used with chemical pesticide because the planthopper resistance to the action of a drug produces, rice planthopper natural enemy is killed, destroys rice
The field ecological balance, seriously undermines field natural control ability, easily causes the rampant again, unmanageable of planthopper.Therefore, develop
Safety, there is the biological pesticide for continuing control action to substitute chemical pesticide to protection paddy fields Environmental security and two packing spaces
Property is significant.
Insect pathogenic fungus is most important insecticidal microorganism monoid, and there is safety, lasting control and insect to be not likely to produce
Drug-fast advantage.Green muscardine fungus（Metarhizium）It is to be used for one of insect pathogenic fungus of pest control earliest, can infects complete
The world there are about 200 various insects.It is more century-old to be utilized to research and the practice of pest control, but not yet controls planthopper registration
Kill planthopper Metarrhizium anisopliae preparation, main cause is a lack of high virulence disinsection fungal bacterial strain and corresponding preparations.Metarhizium anisopliae
CQMa421 is that the one plant of production traits separated on one plant of rice leaf roller bombys batryticatus from natural infection is good, indulges volume to rice
Leaf snout moth's larva has high virulence disinsection fungal, disclosed in Chinese patent CN102212483, the bacterial strain on 02 25th, 2011
China Committee for Culture Collection of Microorganisms's common micro-organisms center（CGMCC）Preservation, deposit number：CGMCC NO.4609.
The bacterial strain and its preparation have no report to the virulence of planthopper with prevention effect.
Wettable powder is a kind of most basic formulations of pesticide, can be made into suspension, is sprayed.Disinsection fungal wettable
Pulvis is by adding a certain amount of surfactant and inserts in fungal spore powder, is made up of sieving.Due to fungal spore
Activity is influenceed by wetting agent, and the dosage of surfactant is often reduced in preparation, causes moistening dispersion effect poor, while product
Storage period is still shorter, rarely exceeds 12 months.The problem of due to moistening dispersing characteristic, bin stability and field efficacy are by ultraviolet
Irradiation influences greatly, though disinsection fungal wettable powder has registration, never widely applies.Therefore, screening domestication is high
Virulent strain and development moisten the good fungus insecticide wettable powder of dispersing characteristic, extend its bin stability, so as to promote
Enter application of the fungus insecticide in planthopper is prevented and treated, advantageously reduce the dosage of chemical pesticide in Rice Production.
The content of the invention
The first object of the present invention is in the application in a kind of Metarhizium anisopliae bacterial strain of offer in planthopper is killed.
Second purpose of the invention is to provide a kind of Metarhizium anisopliae preparation, the resistance to ultraviolet irradiation of said preparation, long shelf-life,
Preventive effect is good.
3rd purpose of the invention be to provide it is a kind of strengthen above-mentioned Metarhizium anisopliae virulence method, without influence its produce spore,
The method of heat-resistant quality.
The present invention seeks to what is be achieved through the following technical solutions：
Application of the Metarhizium anisopliae CQMa421 bacterial strains in the medicament for preparing preventing and treating planthopper, the bacterial strain is to planthopper
With dissemination, fatal rate can reach 32.6%.
Different Metarhizium Strains have specific host, such as open report：Metarhizium anisopliae FI-1045 bacterial strains can invade
Contaminate sugarcane grub（Anoplophora glabripennis）, Metarhizium anisopliae A ESALQ818 bacterial strains can infect Aedes Aegypti
(Aedes aegypti), Metarhizium anisopliae EAMb 09/01-Su bacterial strains can infect Mediterranean fruit fly, Metarhizium anisopliae
PDRL526 bacterial strains can infect radish aphid（Lipaphis erysimi）, Metarhizium anisopliae M2 bacterial strains can infect Aleurodicus dispersus
（Aleurodicus dispersus Russell）, Metarhizium anisopliae ICIPE-30 bacterial strains can infect anopheles costalis
（anopheles gambiae）, the bacterial strains of Metarhizium anisopliae ESC 1 can infect termite, and Metarhizium anisopliae ATCC62176 can be invaded
Contaminate beet root maggot（sugarbeet root maggot）, Metarhizium anisopliae M105 bacterial strains can infect locust, Metarhizium anisopliae
Wide spectrum bacterial strain F-52 can infect grub（Anoplophora glabripennis）, walnut weevil, Xylosandrus germanus
（Xylosandrus germanus）, Metarhizium anisopliae wide spectrum bacterial strain 549 can infect maduca sexta (Manduca sexta),
Aedes Aegypti（Aedes aegypti）, Luo Baici green muscardine fungus wide spectrum bacterial strains ARSEF 23 can infect locust, lepidopterous larvae, first
Worm etc..
Preferably, the medicament of above-mentioned preventing and treating planthopper is by green muscardine fungus CQMa421 xerospore powder 35-52%, diatomite 40-
50%th, nano-silicon 1-2%, sodium carboxymethylcellulose 3-5%, O-30 2-4% and HEL-40 2-4% compositions, with weight percent
Than.
A kind of Metarhizium anisopliae preparation, it is characterised in that：It is by diatomite 40-50%, nano-silicon 1-2%, carboxymethyl
Sodium cellulosate 3-5%, O-30 2-4%, HEL-40 2-4% and green muscardine fungus CQMa421 xerospore powder 35-52% compositions, with weight
Percentage.
Above-mentioned each component is the clear and definite component in this area, and O-30 is peregal -30（Fatty alcohol and ethylene oxide condensation
Thing）, HEL-40 be rilanit special and ethylene oxide condensate, diatomite, nano-silicon（Nano-silicon is that particle diameter is nano level
Silicon）, sodium carboxymethylcellulose be commercially available prod.Green muscardine fungus CQMa421 is disclosed in Chinese patent CN102212483, the bacterium
Strain is on 02 25th, 2011 in China Committee for Culture Collection of Microorganisms's common micro-organisms center（CGMCC）Preservation, guarantor
Hide numbering：CGMCC NO.4609.
The above-mentioned preferred purity of O-30 and HEL-40>95% O-30 and HEL-40；Above-mentioned sodium carboxymethylcellulose（CMC）
It is preferred that purity is more than 95%；Above-mentioned diatomite is preferably that white to light brown loose powder, purity is more than 85%, water content and is less than
1%；Above-mentioned nano-silicon is preferably that particle diameter is less than 50nm；Above-mentioned green muscardine fungus CQMa421 xerospores powder be pure conidia powder, spore content >=
15000000000 spores/gram, water content≤5%；Above in terms of weight/mass percentage composition.
Nano-silicon can be attached to fungal spore surface in above-mentioned Metarhizium anisopliae preparation, effectively obstruct in preparation
High concentration（2-4wt%）O-30 and HEL-40 harmful effect, the bioactivity of spore is effectively ensured.
It is further preferred that Metarhizium anisopliae preparation of the present invention, it is characterised in that：It is by diatomite 40-45%, received
Rice silicon 1.3-2%, sodium carboxymethylcellulose 3-5%, O-30 2-4%, HEL-40 2-4% and green muscardine fungus CQMa421 xerospore powder
40-50% is formed, with percentage by weight.
Above-mentioned green muscardine fungus CQMa421 xerospores powder preferably uses strong virus force Metarhizium anisopliae CQMa421 conidia powders, described
Strong virus force Metarhizium anisopliae CQMa421 conidia powders are obtained by following methods：Metarhizium anisopliae CQMa421 is inoculated in 3-4
Age planthopper larva, raised after inoculation under conditions of 28 DEG C, humidity 50-70%, dead planthopper bombys batryticatus susceptible at first is used
Alcohol surface sterilization, be put into 28 DEG C, humidity be moisturizing culture 3-5 days in 50-70% incubators, its insect surfaces is grown newly
Fresh spore, circulation inoculation as described above tamed for 14-16 times after Metarhizium anisopliae CQMa421 Fresh spores；Sterile
Under the conditions of, Fresh spores are put down in the 1/4SDA culture mediums containing 100 mcg/ml ampicillins after being tamed with oese picking
Lining out；The culture dish rule upset is positioned in 28 DEG C of insulating boxs and cultivated；Selected after 3-4 days grow typical case and
Bacterium colony without miscellaneous bacteria, in colony edge with culture medium one fritter of the acicula picking with mycelia is moved, it is transferred to test tube slant culture medium
Center, 28 DEG C of Metarhizium anisopliae CQMa421 parent species for obtaining strong virus force in incubated 10-15 days, by CQMa421 bacterial strain parent species
It is seeded on 1/4SDA flat boards, 28 DEG C are cultivated 15 days, collect ripe conidium and green muscardine fungus CQMa421 xerospore powder is made.
The preparation method of above-mentioned Metarhizium anisopliae preparation, it is that first green muscardine fungus CQMa421 xerospores powder and nano-silicon exist
Mixed in mixer, sequentially add diatomite, sodium carboxymethylcellulose, O-30 2-4% and HEL-40 2-4%, when adding
Stirring, until each component be mixed thoroughly it is obtained.
A kind of method for strengthening Metarhizium anisopliae virulence, it is characterised in that：Metarhizium anisopliae CQMa421 is inoculated in
3-4 age planthoppers, raised after inoculation under conditions of 28 DEG C, humidity 50-70%, by dead planthopper susceptible at first, be put into 28
DEG C, humidity be moisturizing culture 3-5 days in 50-70% incubators, its insect surfaces is grown Fresh spores, as described above circulation
The Metarhizium anisopliae CQMa421 Fresh spores being inoculated with 14-16 times after being tamed；Aseptically, with oese picking
Fresh spores are rule on the 1/4SDA culture medium flat plates containing 100 mcg/ml ampicillins after domestication, by what is rule
Culture dish upset, which is positioned in 28 DEG C of insulating boxs, cultivates；Selected after 3-4 days grow typical case and the bacterium colony without miscellaneous bacteria, on bacterium colony side
Edge carries the fritter of culture medium one of mycelia with acicula picking is moved, and is transferred to test tube slant culture medium central, 28 DEG C of incubated 10-15
It obtains the Metarhizium anisopliae CQMa421 parent species of strong virus force.
Using the strong virus force Metarhizium anisopliae CQMa421 bacterial strains after the method domestication of present invention enhancing virulence, rice is flown
Lice fatal rate can improve 66.1-67.4%, reach 98.7-100%, the lethal time of 50（LT50）Between 3.9-4.3 days, shorten 57.4-
61.4%, effect is very prominent.This method does not influence the production spore and heat-resistant quality of bacterial strain.
The present invention has following beneficial effect：
Application of the Metarhizium anisopliae CQMa421 bacterial strains of the present invention in the medicament for preparing preventing and treating planthopper, the bacterial strain energy
Planthopper is infected, fatal rate can reach 32.6%, strengthen Metarhizium anisopliae made from the method for virulence using the present invention
CQMa421 bacterial strains, it is very prominent that its fatal rate can reach 98.7-100%, prevention effect.Metarhizium anisopliae preparation of the present invention
In each composition effective cooperation, serve synergistic function, spraying is watered using Metarhizium anisopliae preparation of the present invention, to rice
The field efficacy of plant hopper reaches more than 80%, and said preparation has good wetting and dispersing characteristic, wetting time ＜ 60s, and maintains
Bioactivity in long-term storage, period of storage were very suitable for large-scale production and promoted the use of a large area up to 12 months.
Embodiment
The present invention is specifically described below by embodiment, it is necessary to it is pointed out here that be that following examples are only used
It is further described in the present invention, it is impossible to limiting the scope of the invention is interpreted as, without departing substantially from spirit of the invention
In the case of essence, the modifications or substitutions made to the inventive method, step or condition belong to the scope of the present invention.
Unless otherwise specified, the conventional meanses that technological means used in embodiment is well known to those skilled in the art.
Virulence, sporulating character and heat-resistant quality of the CQMa421 bacterial strains of embodiment 1 after inoculation planthopper domestication
By CQMa421 inoculations on 1/4SDA flat boards, 28 DEG C are trained 15 days, ripe conidium are collected, with 0.05%
（W/V）Tween-80 disperses, and is configured to final concentration of 1 × 107Individual spore/ml suspension.The planthopper of indoor feeding is transferred to
In 500ml triangular flasks with wheat seeding, every bottle of 30-50 planthopper, using spray tower（POTTER, BURKARD company）Spraying
Inoculation, 5 bottles are inoculated with, every bottle of 0.5 ml of inoculation.Surveyed after inoculation in 28 DEG C of room temperature, the raw of humidity 50-70% in room.Selection is felt at first
Die of illness the planthopper bombys batryticatus died, is put into moisturizing culture 3-5 days in 28 DEG C of incubators, its insect surfaces is grown Fresh spores；
Spore circulation inoculation planthopper is collected to be tamed for 12-16 times.Aseptically, Fresh spores after being tamed with oese picking
Rule on the 1/4SDA culture medium flat plates containing 100 mcg/ml ampicillins；The culture dish rule is overturn and placed
Cultivated in 28 DEG C of insulating boxs；Selected after 3-4 days grow typical case and the bacterium colony without miscellaneous bacteria, colony edge with move acicula picking
The fritter of culture medium one with mycelia, is transferred to test tube slant culture medium central, and 28 DEG C incubated 10-15 days.Bacterium after domestication
Conidium, inoculation planthopper are cultivated in strain as stated above, using bacterial strain before domestication as control, are repeated 3 times, record dead borer population, count
Cumulative mortality is calculated, through SPSS softwares LT50（The lethal time of 50）And carry out statistical analysis.
Sporulating character：The μ l of spore suspension 100 for having configured CQMa421 are taken to be seeded on 1/4SDA flat boards, in 28 DEG C of temperature
Cultivated 15 days under the condition of culture of degree, take 5 fungus blocks with random beat of a diameter of 1cm card punch, input is equipped with 50ml's
0.05%（W/V）Disperse in the triangular flask of Tween-80, determine spore concentration using blood counting chamber, the production on unit of account area
Spore amount（Spore count/cm2）, using bacterial strain before domestication as control, if 3 repetitions.Statistical analysis is carried out with SPSS softwares.
Heat-resistant quality：Take the spore suspension for having configured CQMa421.Handle at 50 DEG C 5 hours, respectively taken respectively respectively
100 μ l are seeded on 1/4SDA flat boards, are cultivated at a temperature of 28 DEG C.If nonheat-treated conidiospore suspension is control.It is each
Processing sets three repetitions.After 24 hours, sediments microscope inspection, 200-300 spore of number, the spore count of sprouting is recorded, calculates and sprouts
Rate.Using bacterial strain before domestication as control, if 3 repetitions.Statistical analysis is carried out with SPSS softwares.
Virulence, sporulating character and heat-resistant quality of the CQMa421 bacterial strains of table 1 after inoculation planthopper domestication
Note：In the level of p=0.01, same letter represents that difference is not notable, differs alphabetical significant difference.
From can table 1 to find out, CQMa421 can effectively infect planthopper, 8 days after inoculation, CQMa421 treatment group planthoppers
The death rate is 32.6%, LT50（The lethal time of 50）For 10.1 days；The insecticidal activity of planthopper is significantly increased after the domestication of CQMa421 bacterial strains
By force, 98.7-100% is reached to the fatal rate of planthopper after taming 14-16 times, 66.1-67.45% is improved before relatively taming；In lethal
When（LT50）Between 3.9-4.3 days, shorten 57.4-61.4% before relatively taming.But in 1/4SDA flat boards after the domestication of CQMa421 bacterial strains
On sporulation quantity and heat resistance with domestication before there was no significant difference, illustrate through planthopper tame to bacterial strain CQMa421 sporulating characters
With heat resistance without influence.
The nano-silicon of embodiment 2 is to green muscardine fungus protective effect
After the pure cryptogams of Metarhizium anisopliae CQMa421 are first mixed with nano-silicon by table 2,45% diatomite, 5% are added
O-30, HEL-40 of CMC and different content are mixed.
Ultraviolet protection acts on：By the water-dispersible concentration that is made into of preparation sample for 1 × 107After spore/mL spore suspension, take
100 μ l spore suspensions are applied on 1/4 SDA flat boards, with glass push away it is even after be placed in 290-310nm uviol lamps（978mW/m2）
Lower vertical irradiation 5 hours, then culture 24h hours in 28 DEG C of insulating boxs are placed in, determine germination rate（Method is same as above）, it is repeated 3 times.With
SPSS softwares carry out Treatment Analysis to data.
Store experiment：It will be sealed 2 months at 28 DEG C after preparation sample, the water-dispersible concentration that is made into is 1 × 107Spore
After son/mL spore suspension, germination rate is determined（Method is same as above）, it is repeated 3 times.Treatment Analysis is carried out to data with SPSS softwares.
The nano-silicon of table 2 is to green muscardine fungus protective effect
Note：In the level of p=0.01, same letter represents that difference is not notable, differs alphabetical significant difference.
The nano-silicon that the results are shown in Table 2,1-2% has obvious protective function to Metarhizium anisopliae CQMa421 spores, in O-
When 30 and HEL-40 contents are 2-4%, the spore activity after preserving 2 months is stored more than 90%, is significantly higher than non-plus nano silicon
Processing；Also spore uvioresistant ability is dramatically increased, is laid the foundation for field efficacy stability.
The Metarhizium anisopliae CQMa421 wettable powders of embodiment 3 are prepared and its wetability, storage period and preventive effect.
After the pure cryptogams of Metarhizium anisopliae CQMa421 are first mixed with nano-silicon by table 3, different content diatom is added
Soil, CMC, O-30 and HEL-40.
The wettability measure of Ricinate：Standard water 100mL is taken to inject 250 mL beakers.By this beaker be placed in 25 DEG C ±
In 1 DEG C of water bath with thermostatic control, make its liquid level concordant with the horizontal plane of water-bath.When hard water is to 25 DEG C ± 1 DEG C, accurately weighs 5.0g and treat
Test sample product are placed on surface plate, and whole samples are disposably equably poured over to the liquid of the beaker from the position flushed with beaker mouth
On face, but liquid level is not disturbed excessively.Clocked immediately with stopwatch during additional examination sample, (liquid is stayed in untill sample all wetting
Fine powder film on face can be neglected).Write down wetting time (being accurate to the second).So it is repeated 5 times, takes its average value, be used as this
The wetting time of sample.
Bin stability：Preparation is sealed 12 months at 28 DEG C.The water-dispersible concentration that is made into is 1 × 107Spore/
After mL spore suspension percentage is sprouted by the measure of embodiment 2.
Field efficacy：Select planthopper that rice field occurs（10×10m）, preparation is watered and is dispersed into concentration as 1 × 107
Base portion is sprayed after spore/mL spore suspension, formulation rate 50L/ mus.14 days after dispenser, sampled using parallel great-jump-forward, adjusted per cell
Look into 10: 20 clumps of rice, statistics Revision insect recluced rate and.It is repeated 3 times, takes its average value, the preventive effect as the sample.
The Metarhizium anisopliae CQMa421 wettable powders of table 3 moistening dispersing characteristic, storage period and preventive effect
It the results are shown in Table 3.The wetability of test recipe is good, and wetting time is between ＜ 60s；Storage is stable, at 28 DEG C
Spore activity >=80% of storage 12 months；Field is stable to planthopper preventive effect, and preventive effect reaches more than 80%, can be used as chemical pesticide
Replacement agricultural chemicals.
Claims (4)
1. application of the Metarhizium anisopliae CQMa421 bacterial strains in the medicament for preparing preventing and treating planthopper；The preventing and treating planthopper
Medicament is by green muscardine fungus CQMa421 xerospore powder 35-52%, diatomite 40-50%, nano-silicon 1-2%, sodium carboxymethylcellulose 3-
5%th, O-30 2-4% and HEL-40 2-4% compositions, with percentage by weight.
A kind of 2. Metarhizium anisopliae preparation, it is characterised in that：It is fine by diatomite 40-50%, nano-silicon 1-2%, carboxymethyl
Plain sodium 3-5%, O-30 2-4%, HEL-40 2-4% and green muscardine fungus CQMa421 xerospore powder 35-52% compositions are tieed up, with weight hundred
Divide ratio.
3. Metarhizium anisopliae preparation as claimed in claim 2, it is characterised in that：The O-30 and HEL-40 are purity>95%
O-30 and HEL-40；The sodium carboxymethylcellulose purity is more than 95%；The diatomite is white to light brown loose powder
End, purity are more than 85%, water content and are less than 1%；The nano-silicon is that particle diameter is less than 50nm；The green muscardine fungus CQMa421 xerospores
Powder be pure conidia powder, the spore of spore content >=15,000,000,000/gram, water content≤5%；Above in terms of weight/mass percentage composition.
4. the Metarhizium anisopliae preparation as described in Claims 2 or 3, it is characterised in that：It is by diatomite 40-45%, nano-silicon
1.3-2%, sodium carboxymethylcellulose 3-5%, O-30 2-4%, HEL-40 2-4% and green muscardine fungus CQMa421 xerospore powder 40-
50% composition, with percentage by weight.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510084106.9A CN104604947B (en) | 2015-02-16 | 2015-02-16 | One kind kills planthopper Metarhizium anisopliae preparation and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510084106.9A CN104604947B (en) | 2015-02-16 | 2015-02-16 | One kind kills planthopper Metarhizium anisopliae preparation and its application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104604947A CN104604947A (en) | 2015-05-13 |
| CN104604947B true CN104604947B (en) | 2017-11-24 |
Family
ID=53139820
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510084106.9A Active CN104604947B (en) | 2015-02-16 | 2015-02-16 | One kind kills planthopper Metarhizium anisopliae preparation and its application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104604947B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105274007B (en) * | 2015-07-27 | 2018-11-27 | 华南农业大学 | One plant of Metarhizium anisopliae var. Anisopliae MaTS02 and its application in terms of preventing and treating Bemisia tabaci |
| CN109526987B (en) * | 2018-11-21 | 2021-11-26 | 重庆市农业科学院 | Method for preventing and treating rice planthopper by combined action of metarhizium anisopliae and sex attractant |
| CN110547304A (en) * | 2019-09-24 | 2019-12-10 | 重庆谷百奥生物研究院有限公司 | Beauveria bassiana and amitraz compounded trialeurodes vaporariorum killing composition |
| CN111088172A (en) * | 2020-03-06 | 2020-05-01 | 中国水稻研究所 | Method for enhancing weeding toxicity of helminthosporium peregrinum spores |
| CN111557307A (en) * | 2020-05-27 | 2020-08-21 | 重庆谷百奥生物研究院有限公司 | Application of metarhizium anisopliae in preventing and treating greenhouse artichoke by using bees as carrier |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100367858C (en) * | 2005-09-02 | 2008-02-13 | 中国科学院动物研究所 | Baculoviral insecticidal suspending agent comprising nano material and preparation method thereof |
| CN101564047B (en) * | 2009-05-27 | 2012-02-15 | 重庆大学 | Disinsection fungi emulsifiable powder |
| CN102212483B (en) * | 2011-04-26 | 2012-08-01 | 重庆大学 | Pesticidal Metarhiziumanisopliaevar. anisopliae strain and application thereof |
| CN103160450A (en) * | 2011-12-15 | 2013-06-19 | 河北农业大学 | Broad-spectrum and highly effective new Bacillus thuringiensis strain, its bacterial agent and application |
-
2015
- 2015-02-16 CN CN201510084106.9A patent/CN104604947B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN104604947A (en) | 2015-05-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104621185B (en) | A kind of application of Metarhizium anisopliae bacterial strain in the medicament for preparing killing aphids | |
| CN104604947B (en) | One kind kills planthopper Metarhizium anisopliae preparation and its application | |
| CN103749441B (en) | Isaria fumosorosea wettable powder as well as preparation method and application thereof | |
| CN105802863B (en) | Trichoderma harzianum fermentation process, wettable powder and application thereof | |
| CN106967618B (en) | Strain of Beauveria bassiana EHM-068 and its application | |
| CN105039167B (en) | A kind of beauveria bassiana DSXJ 07 and its application | |
| CN112280716B (en) | Bacillus altitudinis YG045 and application thereof | |
| CN112143686B (en) | Bacillus altitudinis ST15 for antagonizing xanthomonas oryzae and application thereof | |
| CN104611240A (en) | Application of Metarhizium anisopliae strain in preparation of insecticide for vegetable pest insects | |
| CN104745485A (en) | Application of metarhizium anisopliae strains to preparation of pesticide for killing lissorhoptrus oryzophilus kuschel | |
| JP4247841B2 (en) | Filamentous fungi having control ability against gramineous plant diseases, control agents using the same, control methods, and biological materials | |
| CN107955796B (en) | Red silk ribbon attached to an official seal or a medal aspergillus Q-1 and its application in termite control | |
| CN104611241A (en) | Preparation for killing ostrinia nubilalis metarhizium anisopliae and application of preparation | |
| CN101889583B (en) | Anti-Cydia pomonella biological pesticide and preparation method thereof | |
| CN105331561B (en) | One plant of radiation rhizobium for preventing plant root cancer and its application | |
| CN101513193A (en) | Compound desinsection primary agent and pesticide consisting of verticillium lecanii and beta-cypermethrin | |
| CN112753716A (en) | Pesticide composition and application thereof in aspect of preventing and treating phyllotreta striolata | |
| CN109699683B (en) | Talcum matrix Java cordyceps militaris spore preparation | |
| Afandhi et al. | The diversity of entomopathogenic fungi collected from leaves and rhizospheres of rice implementing integrated pest management | |
| CN106993631A (en) | A kind of knife spore Verticillium dahliae wettable powder and its application | |
| CN106912501B (en) | The compounded pesticides of Lecanicillium lecanii and rotenone | |
| CN106962408B (en) | A kind of gradually narrow Verticillium dahliae and the compounded pesticides of Osthole | |
| KR100961786B1 (en) | Biological control of plant diseases using burkholderia pyrrocinia cab08106-4 | |
| EP3476930B1 (en) | Pseudozyma | |
| CN106982859B (en) | A kind of compounded pesticides of Lecanicillium lecanii and Osthole |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |