CN104604947A - Rice planthopper killing metarhizium anisopliae preparation and application thereof - Google Patents
Rice planthopper killing metarhizium anisopliae preparation and application thereof Download PDFInfo
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Abstract
The invention relates to application of a metarhizium anisopliae CQMa421 strain in preparation of a medicament for preventing and treating rice planthoppers. The medicament for preventing and treating the rice planthoppers is prepared from the following components by weight percent: 35-52% of metarhizium anisopliae CQMa421 dry spore powder, 40-50% of diatomite, 1-2% of nanometer silicon, 3-5% of sodium carboxymethylcellulose, 2-4% of O-30 and 2-4% of HEL-40. The rice planthoppers can be infected by the metarhizium anisopliae CQMa421 strain, and the fatality rate can reach 32.6 percent. The metarhizium anisopliae CQMa421 strain prepared by a toxicity increasing method has the fatality rate of 98.7-100%, LT50 is shortened by 57.4-61.4%, and the effect is very prominent. The components of the metarhizium anisopliae preparation are effectively matched to play a synergistic interaction role, and filed efficacy on the rice planthoppers can reach 80% above by spraying the metarhizium anisopliae preparation added with water. The preparation has good ultraviolet resistance and dispersing and wetting properties, the biological activity in long-term storage is kept, and the storage time is up to 12 months.
Description
Technical field
The present invention relates to a kind of new opplication of Metarhizium anisopliae bacterial strain, be specifically related to one and kill rice fulgorid Metarhizium anisopliae preparation and application thereof, belong to field of biological pesticide.
Technical background
Rice fulgorid is the main migratory pest of paddy rice, is domesticly distributed widely in main rice district, the whole nation, and serious threatens China's grain-production.At present, mainly use broad spectrum activity chemical pesticide and control rice fulgorid, not only directly affect the safety of health and culture fishery, and due to rice fulgorid pesticide resistance produce and chemical pesticide use in a large number, kill and wound rice planthopper natural enemy, destruction rice field ecology balances, and seriously undermines field natural control ability, easily causes the wildness again of rice fulgorid, is difficult to control.Therefore, exploitation safely, the biopesticide with lasting control action substitute chemical pesticide to protection paddy fields Environmental security and bio-diversity significant.
Insect pathogenic fungus is most important insecticidal microorganism monoid, has the advantage that safety, lasting control and insect not easily develop immunity to drugs.Green muscardine fungus (Metarhizium) is that can infect the whole world about has 200 various insects the earliest for one of the insect pathogenic fungus of pest control.The Researching and practicing utilizing it to carry out pest control is more century-old, but not yet control rice fulgorid registration kill rice fulgorid Metarrhizium anisopliae preparation, main cause lacks high virulence disinsection fungal bacterial strain and corresponding preparations.Metarhizium anisopliae CQMa421 be the strain production traits that a strain separates from the rice leaf roller bombys batryticatus of natural infection good, to rice leaf roller, there is high virulence disinsection fungal, open in Chinese patent CN102212483, this bacterial strain on 02 25th, 2011 in China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC) preservation, deposit number: CGMCC NO.4609.This bacterial strain and preparation thereof have no report to the virulence of rice fulgorid and control efficiency.
Wetting powder is the most basic a kind of formulations of pesticide, can be made into suspension, sprays.Disinsection fungal wetting powder adds a certain amount of surfactant and inserts by fungal spore powder, makes by sieving.Because fungal spore activity is by the impact of wetting agent, often reduce the consumption of surfactant in preparation, cause moistening dispersion effect poor, product is still shorter for storage period simultaneously, rarely exceeds 12 months.Because the problem of moistening dispersing characteristic, bin stability and field efficacy affect greatly by ultraviolet irradiation, though disinsection fungal wetting powder has registration, never widely apply.Therefore, the fungus insecticide wetting powder that screening domestication supper toxic strain is good with the moistening dispersing characteristic of development, extend its bin stability, thus promote the application of fungus insecticide in control rice fulgorid, be conducive to the consumption reducing chemical pesticide in Rice Production.
Summary of the invention
The first object of the present invention is to provide a kind of Metarhizium anisopliae bacterial strain killing the application in rice fulgorid.
The present invention second object is to provide a kind of Metarhizium anisopliae preparation, and the resistance to ultraviolet irradiation of said preparation, long shelf-life, preventive effect are good.
The present invention the 3rd object is that providing a kind of strengthens above-mentioned Metarhizium anisopliae virulence method, and does not affect the method that it produces spore, heat-resistant quality.
The present invention seeks to be achieved through the following technical solutions:
The application of Metarhizium anisopliae CQMa421 bacterial strain in the medicament of preparation control rice fulgorid, this bacterial strain has dissemination to rice fulgorid, and lethality rate can reach 32.6%.
Different Metarhizium Strains has specific host, as open report: Metarhizium anisopliae FI-1045 bacterial strain can infect sugarcane grub (Anoplophora glabripennis), Metarhizium anisopliae A ESALQ818 bacterial strain can infect Aedes Aegypti (Aedes aegypti), Metarhizium anisopliae EAMb 09/01-Su bacterial strain can infect Mediterranean fruit fly, Metarhizium anisopliae PDRL526 bacterial strain can infect radish aphid (Lipaphis erysimi), Metarhizium anisopliae M2 bacterial strain can infect Aleurodicus dispersus (Aleurodicus dispersus Russell), Metarhizium anisopliae ICIPE-30 bacterial strain can infect anopheles costalis (anopheles gambiae), Metarhizium anisopliae ESC 1 bacterial strain can infect termite, Metarhizium anisopliae ATCC62176 can infect beet root maggot (sugarbeet root maggot), Metarhizium anisopliae M105 bacterial strain can infect locust, Metarhizium anisopliae wide spectrum bacterial strain F-52 can infect grub (Anoplophora glabripennis), walnut weevil, Xylosandrus germanus (Xylosandrus germanus), Metarhizium anisopliae wide spectrum bacterial strain 549 can infect maduca sexta (Manduca sexta), Aedes Aegypti (Aedes aegypti), Luo Baici green muscardine fungus wide spectrum bacterial strain ARSEF 23 can infect locust, lepidopterous larvae, beetle etc.
Preferably, the medicament of above-mentioned control rice fulgorid is made up of, all with percentage by weight green muscardine fungus CQMa421 xerospore powder 35-52%, diatomite 40-50%, nano-silicon 1-2%, sodium carboxymethylcellulose 3-5%, O-30 2-4% and HEL-40 2-4%.
A kind of Metarhizium anisopliae preparation, is characterized in that: it is made up of, all with percentage by weight diatomite 40-50%, nano-silicon 1-2%, sodium carboxymethylcellulose 3-5%, O-30 2-4%, HEL-40 2-4% and green muscardine fungus CQMa421 xerospore powder 35-52%.
Above-mentioned each component is the clear and definite component in this area, O-30 is peregal-30(fatty alcohol and ethylene oxide condensate), HEL-40 is rilanit special and ethylene oxide condensate, diatomite, nano-silicon (nano-silicon is particle diameter is nano level silicon), sodium carboxymethylcellulose are commercially available prod.Green muscardine fungus CQMa421 is open in Chinese patent CN102212483, this bacterial strain on 02 25th, 2011 at China Committee for Culture Collection of Microorganisms's common micro-organisms center (CGMCC) preservation, deposit number: CGMCC NO.4609.
O-30 and HEL-40 of the preferred purity >95% of above-mentioned O-30 and HEL-40; Above-mentioned sodium carboxymethylcellulose (CMC) preferably purity is greater than 95%; Above-mentioned diatomite is preferably white to light brown loose powder, and purity is greater than 85%, water content is less than 1%; Above-mentioned nano-silicon is preferably particle diameter and is less than 50nm; Above-mentioned green muscardine fungus CQMa421 xerospore powder be pure conidial powder, spore content >=15,000,000,000 spores/gram, water content≤5%; Above all in mass percentage.
In above-mentioned Metarhizium anisopliae preparation, nano-silicon can be attached to fungal spore surface, effectively intercept the harmful effect of preparation middle and high concentration (2-4wt%) O-30 and HEL-40, effectively ensure that the biologically active of spore.
Further preferably, Metarhizium anisopliae preparation of the present invention, it is characterized in that: it is made up of, all with percentage by weight diatomite 40-45%, nano-silicon 1.3-2%, sodium carboxymethylcellulose 3-5%, O-30 2-4%, HEL-40 2-4% and green muscardine fungus CQMa421 xerospore powder 40-50%.
Above-mentioned green muscardine fungus CQMa421 xerospore powder preferably adopts strong virus force Metarhizium anisopliae CQMa421 conidial powder, described strong virus force Metarhizium anisopliae CQMa421 conidial powder is obtained by following methods: Metarhizium anisopliae CQMa421 is inoculated in 3-4 rice fulgorid in age larva, at 28 DEG C after inoculation, raise under the condition of humidity 50-70%, by the rice fulgorid bombys batryticatus alcohol surface sterilization of susceptible death at first, put into 28 DEG C, humidity is that in 50-70% incubator, 3-5 days is cultivated in moisturizing, its insect surfaces is made to grow Fresh spores, circulation inoculation described above obtains the Metarhizium anisopliae CQMa421 Fresh spores after taming for 14-16 times, aseptically, ruling containing on the 1/4SDA culture medium flat plate of 100 mcg/ml ampicillins with Fresh spores after the domestication of oese picking, the culture dish of having rule upset is positioned in 28 DEG C of insulating boxs and cultivates, the typical case that grows is selected and without the bacterium colony of miscellaneous bacteria after 3-4 days, at colony edge with moving medium one fritter of acicula picking with mycelia, proceed to test tube slant medium central authorities, 28 DEG C of Metarhizium anisopliae CQMa421 mothers obtaining strong virus force for constant temperature culture 10-15 days plant, CQMa421 bacterial strain mother being planted is seeded on 1/4SDA flat board, cultivate 15 days, collect ripe conidium and obtain green muscardine fungus CQMa421 xerospore powder for 28 DEG C.
The preparation method of above-mentioned Metarhizium anisopliae preparation, first green muscardine fungus CQMa421 xerospore powder and nano-silicon are mixed in mixer, add diatomite, sodium carboxymethylcellulose, O-30 2-4% and HEL-40 2-4% more successively, stir while adding, until each component mixes obtained completely.
A kind of method strengthening Metarhizium anisopliae virulence, it is characterized in that: Metarhizium anisopliae CQMa421 is inoculated in 3-4 rice fulgorid in age, after inoculation 28 DEG C, raise under the condition of humidity 50-70%, by the rice fulgorid of susceptible death at first, put into 28 DEG C, humidity be the moisturizing of 50-70% incubator cultivate 3-5 days, make its insect surfaces grow Fresh spores, circulation inoculation described above obtains the Metarhizium anisopliae CQMa421 Fresh spores after taming for 14-16 time; Aseptically, ruling containing on the 1/4SDA culture medium flat plate of 100 mcg/ml ampicillins with Fresh spores after the domestication of oese picking, the culture dish of having rule upset is being positioned in 28 DEG C of insulating boxs and cultivates; The typical case grown is selected and without the bacterium colony of miscellaneous bacteria after 3-4 day, at colony edge with moving medium one fritter of acicula picking with mycelia, proceed to test tube slant medium central authorities, 28 DEG C of Metarhizium anisopliae CQMa421 mothers obtaining strong virus force for constant temperature culture 10-15 days plant.
Strong virus force Metarhizium anisopliae CQMa421 bacterial strain after adopting the present invention to strengthen the method domestication of virulence, can improve 66.1-67.4% to rice fulgorid lethality rate, reach 98.7-100%, the lethal time of 50 (LT
50) between 3.9-4.3 days, shorten 57.4-61.4%, effect is very outstanding.The method does not affect product spore and the heat-resistant quality of bacterial strain.
The present invention has following beneficial effect:
The application of Metarhizium anisopliae CQMa421 bacterial strain of the present invention in the medicament of preparation control rice fulgorid, this bacterial strain can infect rice fulgorid, lethality rate can reach 32.6%, adopt the present invention to strengthen the obtained Metarhizium anisopliae CQMa421 bacterial strain of the method for virulence, its lethality rate can reach 98.7-100%, control efficiency is very outstanding.In Metarhizium anisopliae preparation of the present invention each composition effective cooperation, serve synergistic function; Metarhizium anisopliae preparation of the present invention is adopted to be watered spraying; more than 80% is reached to the field efficacy of rice fulgorid; said preparation has good wetting and dispersing characteristic, wetting time < 60s; and the biologically active maintained in long term storage; period of storage can reach 12 months, is very suitable for large-scale production and spread use.
Embodiment
Below by embodiment, the present invention is specifically described; what be necessary to herein means out is that following examples are only used to further illustrate the present invention; limiting the scope of the invention can not be interpreted as; without departing from the spirit and substance of the case in the present invention; the amendment do the inventive method, step or condition or replacement, all belong to scope of the present invention.
If do not specialize, the conventional means that technological means used in embodiment is well known to those skilled in the art.
virulence, sporulating character and the heat-resistant quality of embodiment 1 CQMa421 bacterial strain after the domestication of inoculation rice fulgorid
By CQMa421 inoculation on 1/4SDA flat board, train 15 days, collect ripe conidium, with 0.05%(W/V for 28 DEG C) Tween-80 dispersion, being configured to final concentration is 1 × 10
7the suspension of individual spore/ml.The rice fulgorid of indoor feeding being proceeded to length has in the 500ml triangular flask of wheat seeding, and every bottle of 30-50 rice fulgorid adopts spray tower (POTTER, BURKARD company) spray inoculation, inoculates 5 bottles, every bottle graft kind 0.5 ml.Room temperature 28 DEG C after inoculation, the raw of humidity 50-70% is surveyed in room.Select the rice fulgorid bombys batryticatus of susceptible death at first, put into 28 DEG C of incubator moisturizings and cultivate 3-5 days, make its insect surfaces grow Fresh spores; Collect spore circulation inoculation rice fulgorid to tame for 12-16 time.Aseptically, ruling containing on the 1/4SDA culture medium flat plate of 100 mcg/ml ampicillins with Fresh spores after the domestication of oese picking; The culture dish of having rule upset is positioned in 28 DEG C of insulating boxs and cultivates; Select the typical case grown after 3-4 day and without the bacterium colony of miscellaneous bacteria, at colony edge with moving medium one fritter of acicula picking with mycelia, proceed to test tube slant medium central authorities, 28 DEG C of constant temperature culture 10-15 days.Bacterial strain after domestication cultivates conidium, inoculation rice fulgorid as stated above, to tame front bacterial strain for contrast, repeats 3 times, records dead borer population, calculate cumulative mortality, through SPSS software LT
50(lethal time of 50) also carries out statistical analysis.
Sporulating character: get the spore suspension 100 μ l configuring CQMa421 and be seeded on 1/4SDA flat board, cultivate 15 days under the condition of culture of 28 DEG C of temperature, beat at random with the card punch that diameter is 1cm and get 5 bacterium blocks, drop into and the 0.05%(W/V of 50ml be housed) disperse in the triangular flask of Tween-80, blood counting chamber is utilized to measure spore concentration, the sporulation quantity (spore count/cm on unit of account area
2), to tame front bacterial strain for contrast, if 3 times are repeated.Statistical analysis is carried out with SPSS software.
Heat-resistant quality: get the spore suspension configuring CQMa421.At 50 DEG C, process 5 hours respectively respectively, respectively get 100 μ l and be seeded on 1/4SDA flat board, cultivate at 28 DEG C of temperature.If nonheat-treated conidiospore suspension is contrast.Three repetitions are established in each process.After 24 hours, sediments microscope inspection, number 200-300 spore, the spore count that record is sprouted, calculates germination rate.To tame front bacterial strain for contrast, if 3 times are repeated.Statistical analysis is carried out with SPSS software.
Virulence, sporulating character and the heat-resistant quality of table 1 CQMa421 bacterial strain after the domestication of inoculation rice fulgorid
Note: in the level of p=0.01, same letter represents that difference is not remarkable, not same letter significant difference.
From can table 1 to find out, CQMa421 effectively can infect rice fulgorid, inoculates latter 8 days, and CQMa421 processed group rice fulgorid lethality is 32.6%, LT
50(lethal time of 50) is 10.1 days; After the domestication of CQMa421 bacterial strain, the insecticidal activity of rice fulgorid is significantly strengthened, after taming 14-16 time, 98.7-100% is reached to the lethality rate of rice fulgorid, comparatively improve 66.1-67.45% before domestication; The lethal time of 50 (LT
50) between 3.9-4.3 days, comparatively shorten 57.4-61.4% before domestication.But there was no significant difference before sporulation quantity on 1/4SDA flat board and warm tolerance and domestication after the domestication of CQMa421 bacterial strain, illustrates and to tame bacterial strain CQMa421 sporulating character and warm tolerance without impact through rice fulgorid.
embodiment 2nano-silicon is to green muscardine fungus protective effect
After first being mixed with nano-silicon by pure for Metarhizium anisopliae CQMa421 cryptogam by table 2, then add O-30, HEL-40 mixing of 45% diatomite, 5%CMC and different content.
Ultraviolet protection effect: it is 1 × 10 that the aqueous dispersion of preparation sample is made into concentration
7after the spore suspension of spore/mL, get 100 μ l spore suspensions and be applied on 1/4 SDA flat board, with glass push away even after be placed in 290-310nm uviol lamp (978mW/m
2) under vertical irradiation 5 hours, then be placed in 28 DEG C of insulating boxs and cultivate 24h hour, measure germination rate (method is the same), repeat 3 times.With SPSS software, Treatment Analysis is carried out to data.
Store experiment: preserve 2 months at 28 DEG C of lower seals by after preparation sample, being made into concentration with aqueous dispersion is 1 × 10
7after the spore suspension of spore/mL, measure germination rate (method is the same), repeat 3 times.With SPSS software, Treatment Analysis is carried out to data.
Table 2 nano-silicon is to green muscardine fungus protective effect
Note: in the level of p=0.01, same letter represents that difference is not remarkable, not same letter significant difference.
The nano-silicon that the results are shown in Table 2,1-2% has obvious protective effect to Metarhizium anisopliae CQMa421 spore, and when O-30 and HEL-40 content is 2-4%, the spore of storage preservation after 2 months is active more than 90%, is significantly higher than the process of non-plus nano silicon; Also spore uvioresistant ability is significantly increased, for field efficacy stability lays the foundation.
embodiment 3the preparation of Metarhizium anisopliae CQMa421 wetting powder and wetability, storage period and preventive effect.
After first being mixed with nano-silicon by pure for Metarhizium anisopliae CQMa421 cryptogam by table 3, then add different content diatomite, CMC, O-30 and HEL-40.
The wettability of Ricinate measures: get standard water 100mL and inject 250 mL beakers.This beaker is placed in the water bath with thermostatic control of 25 DEG C ± 1 DEG C, makes its liquid level concordant with the horizontal plane of water-bath.When hard water to 25 DEG C ± 1 DEG C, accurately take 5.0g testing sample and be placed on surface plate, whole sample is poured over the liquid level of this beaker equably from the position flushed with beaker mouth is disposable, but not disturbance liquid level excessively.Clock with stopwatch immediately when adding sample, till sample is all wetting (the fine powder film stayed on liquid level can be ignored).Write down wetting time (being accurate to second).Repetition like this 5 times, gets its mean value, as the wetting time of this sample.
Bin stability: preparation is preserved 12 months at 28 DEG C of lower seals.Being made into concentration with aqueous dispersion is 1 × 10
7measure by embodiment 2 after the spore suspension of spore/mL and sprout percentage.
Field efficacy: select rice fulgorid generation paddy rice field (10 × 10m), being watered by preparation and being dispersed into concentration is 1 × 10
7base portion spraying after the spore suspension of spore/mL, formulation rate 50L/ mu.After dispenser 14 days, adopt parallel great-jump-forward to sample, 10: 20 clumps of rice were investigated in every community, statistics Revision insect recluced rate and
.Repeat 3 times, get its mean value, as the preventive effect of this sample.
The moistening dispersing characteristic of table 3 Metarhizium anisopliae CQMa421 wetting powder, storage period and preventive effect
The results are shown in Table 3.The wetability of test recipe is good, and wetting time is between < 60s; Stable storage, stores spore activity >=80% of 12 months at 28 DEG C; Field is stablized rice fulgorid preventive effect, and preventive effect reaches more than 80%, can as the alternative agricultural chemicals of chemical pesticide.
Claims (7)
1. the application of Metarhizium anisopliae CQMa421 bacterial strain in the medicament of preparation control rice fulgorid.
2. apply as claimed in claim 1, it is characterized in that: the medicament of described control rice fulgorid is made up of, all with percentage by weight green muscardine fungus CQMa421 xerospore powder 35-52%, diatomite 40-50%, nano-silicon 1-2%, sodium carboxymethylcellulose 3-5%, O-30 2-4% and HEL-40 2-4%.
3. apply as claimed in claim 1 or 2, it is characterized in that: described green muscardine fungus CQMa421 xerospore powder adopts strong virus force Metarhizium anisopliae CQMa421 conidial powder, described strong virus force Metarhizium anisopliae CQMa421 conidial powder is obtained by following methods: Metarhizium anisopliae CQMa421 is inoculated in 3-4 rice fulgorid in age larva, at 28 DEG C after inoculation, raise under the condition of humidity 50-70%, by the rice fulgorid bombys batryticatus alcohol surface sterilization of susceptible death at first, put into 28 DEG C, humidity is that in 50-70% incubator, moisturizing is cultivated 3-5 days, its insect surfaces is made to grow Fresh spores, circulation inoculation described above obtains the Metarhizium anisopliae CQMa421 Fresh spores after taming for 14-16 time, aseptically, ruling containing on the 1/4SDA culture medium flat plate of 100 mcg/ml ampicillins with Fresh spores after the domestication of oese picking, the culture dish of having rule upset is positioned in 28 DEG C of insulating boxs and cultivates, the typical case that grows is selected and without the bacterium colony of miscellaneous bacteria after 3-4 days, at colony edge with moving medium one fritter of acicula picking with mycelia, proceed to test tube slant medium central authorities, 28 DEG C of Metarhizium anisopliae CQMa421 mothers obtaining strong virus force for constant temperature culture 10-15 days plant, CQMa421 bacterial strain mother being planted is seeded on 1/4SDA flat board, cultivate 15 days, collect ripe conidium and obtain green muscardine fungus CQMa421 xerospore powder for 28 DEG C.
4. a Metarhizium anisopliae preparation, it is characterized in that: it is made up of, all with percentage by weight diatomite 40-50%, nano-silicon 1-2%, sodium carboxymethylcellulose 3-5%, O-30 2-4%, HEL-40 2-4% and green muscardine fungus CQMa421 xerospore powder 35-52%.
5. Metarhizium anisopliae preparation as claimed in claim 4, is characterized in that: described O-30 and HEL-40 is O-30 and HEL-40 of purity >95%; Described sodium carboxymethylcellulose purity is greater than 95%; Described diatomite be white to light brown loose powder, purity is greater than 85%, water content is less than 1%; Described nano-silicon is that particle diameter is less than 50nm; Described green muscardine fungus CQMa421 xerospore powder be pure conidial powder, spore content >=15,000,000,000 spores/gram, water content≤5%; Above all in mass percentage.
6. Metarhizium anisopliae preparation as described in claim 4 or 5, it is characterized in that: it is made up of, all with percentage by weight diatomite 40-45%, nano-silicon 1.3-2%, sodium carboxymethylcellulose 3-5%, O-30 2-4%, HEL-40 2-4% and green muscardine fungus CQMa421 xerospore powder 40-50%.
7. as claim 4, Metarhizium anisopliae preparation described in 5 or 6, it is characterized in that: described green muscardine fungus CQMa421 xerospore powder is strong virus force Metarhizium anisopliae CQMa421 conidial powder, described strong virus force Metarhizium anisopliae CQMa421 conidial powder is obtained by following methods: Metarhizium anisopliae CQMa421 is inoculated in 3-4 rice fulgorid in age larva, at 28 DEG C after inoculation, raise under the condition of humidity 50-70%, by the rice fulgorid bombys batryticatus alcohol surface sterilization of susceptible death at first, put into 28 DEG C, humidity is that in 50-70% incubator, 3-5 days is cultivated in moisturizing, its insect surfaces is made to grow Fresh spores, circulation inoculation described above obtains the Metarhizium anisopliae CQMa421 Fresh spores after taming for 14-16 time, aseptically, ruling containing on the 1/4SDA culture medium flat plate of 100 mcg/ml ampicillins with Fresh spores after the domestication of oese picking, the culture dish of having rule upset is positioned in 28 DEG C of insulating boxs and cultivates, the typical case grown is selected and without the bacterium colony of miscellaneous bacteria after 3-4 day, at colony edge with moving medium one fritter of acicula picking with mycelia, proceed to test tube slant medium central authorities, 28 DEG C of Metarhizium anisopliae CQMa421 mothers obtaining strong virus force for constant temperature culture 10-15 days plant, CQMa421 bacterial strain mother being planted is seeded on 1/4SDA flat board, cultivate 15 days, collect ripe conidium and obtain green muscardine fungus CQMa421 xerospore powder for 28 DEG C.
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| CN109526987A (en) * | 2018-11-21 | 2019-03-29 | 重庆市农业科学院 | A method of planthopper is prevented and treated using green muscardine fungus and sex attractant synergy |
| CN110547304A (en) * | 2019-09-24 | 2019-12-10 | 重庆谷百奥生物研究院有限公司 | Beauveria bassiana and amitraz compounded trialeurodes vaporariorum killing composition |
| CN111088172A (en) * | 2020-03-06 | 2020-05-01 | 中国水稻研究所 | Method for enhancing weeding toxicity of helminthosporium peregrinum spores |
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| CN105274007A (en) * | 2015-07-27 | 2016-01-27 | 华南农业大学 | Metarhizium anisopliae var. microsporus MaTS02 and application thereof in prevention and treatment of bemisia tabaci |
| CN105274007B (en) * | 2015-07-27 | 2018-11-27 | 华南农业大学 | One plant of Metarhizium anisopliae var. Anisopliae MaTS02 and its application in terms of preventing and treating Bemisia tabaci |
| CN109526987A (en) * | 2018-11-21 | 2019-03-29 | 重庆市农业科学院 | A method of planthopper is prevented and treated using green muscardine fungus and sex attractant synergy |
| CN109526987B (en) * | 2018-11-21 | 2021-11-26 | 重庆市农业科学院 | Method for preventing and treating rice planthopper by combined action of metarhizium anisopliae and sex attractant |
| CN110547304A (en) * | 2019-09-24 | 2019-12-10 | 重庆谷百奥生物研究院有限公司 | Beauveria bassiana and amitraz compounded trialeurodes vaporariorum killing composition |
| CN111088172A (en) * | 2020-03-06 | 2020-05-01 | 中国水稻研究所 | Method for enhancing weeding toxicity of helminthosporium peregrinum spores |
| CN111557307A (en) * | 2020-05-27 | 2020-08-21 | 重庆谷百奥生物研究院有限公司 | Application of metarhizium anisopliae in preventing and treating greenhouse artichoke by using bees as carrier |
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