CN109526987A - A method of planthopper is prevented and treated using green muscardine fungus and sex attractant synergy - Google Patents
A method of planthopper is prevented and treated using green muscardine fungus and sex attractant synergy Download PDFInfo
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- CN109526987A CN109526987A CN201811388222.XA CN201811388222A CN109526987A CN 109526987 A CN109526987 A CN 109526987A CN 201811388222 A CN201811388222 A CN 201811388222A CN 109526987 A CN109526987 A CN 109526987A
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- planthopper
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
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Abstract
The invention discloses a kind of methods using green muscardine fungus and sex attractant synergy prevention and treatment planthopper, it belongs to rice grub green prevention and control field.Realized by following steps: step 1: sex pheromone being dissolved in n-hexane and is configured to trapping bait, and is loaded in rubber stopper as lure；The sex pheromone is the mixture of 14 carbon triolefin acetic acid esters, tetradecadienyl acetate, 16 carbon triolefin -2- ketone, 16 carbon diene -2- ketone；Step 2: the lure of step 1 is placed in trapping box, trapping box is put into rice field；Step 3: by muscardine spore loss in trapping box, trapping planthopper is concentrated in implementation.The present invention is for trapping and killing planthopper.
Description
Technical field
The invention belongs to rice grub green prevention and control fields；Insect pathogenic fungus green muscardine fungus and elder brother are utilized more particularly to a kind of
Worm sex attractant synergy, easy, nontoxic, efficient rice grub planthopper (NilaparvatalugensStal) are prevented
Control method.
Background technique
Planthopper NilaparvatalugensIt is a kind of important migratory pest in the Rice Production of Asia.Due to
The factors such as susceptible worm rice large area is planted and chemical fertilizer, pesticide largely use improve brown paddy plant hopper initial population quantity and increasing
Long rate, so as to cause hazard area big generation year after year；Meanwhile planthopper belongs to r- countermeasure type pest, has high intrinsic increasing
Long rate has stronger adaptability to environment, thus its easy outburst is caused disaster and the characteristics of causing harm of migrating makes insecticide chemistry anti-
It controls and occupies leading position in planthopper emergency flight control.The easy pollution environment of long-term a large amount of and unreasonable uses of chemical agent,
Killing natural enemy and pest develop drug resistance and wildness again.
Metarhizium Fungi Imperfecti, clump obstruct Zoopagales, Cong Gengmei section, Metarhizium, are a kind of insect pathogenic bacterias of wide spectrum, tool
There is certain specificity, to person poultry harmless, while it is excellent also to there is free from environmental pollution, noresidue, pest will not develop drug resistance etc.
Point.Mode of action pest control population is mutually infected using green stiff fungus infection individual in field.Currently, some Metarhizium Strains are
Exploitation is fungus insecticide, and development application, such as 10,000,000,000 spores/ml Metarhizium anisopliae oil-suspending agent are used to prevent and treat
Migratory locusts.
Exclusive use green muscardine fungus effect is slow and preventive effect is unstable, and green muscardine fungus is mixed with decis, phoxim, d ichlorbenzuron etc.
Desinsection speed can be improved, also have the report that green muscardine fungus and pyrethrin pesticide are made to composition, but general chemical agent pair
Spore survival and sprouting have an impact.The sex attractant of planthopper, usually its sex pheromone, main component are 16 carbon diene-
2- ketone, 16 carbon triolefin -2- ketone.It can be used as a kind of mode of rice field planthopper insect pest situation monitoring using sex pheromone lure technology,
It can largely be synthesized chemically, economically be laid a good foundation for application.Draw currently with insect pathogenic bacteria and property
The method of lure synergy field control planthopper is rarely reported.
Summary of the invention
Insect pathogenic fungus green muscardine fungus and insect sex attractant (sex pheromone) are utilized it is an object of the invention to provide a kind of
The method of synergy prevention and treatment planthopper.Method of the invention can effective prevention and control planthopper.
The technical solution of the present invention is as follows: a kind of method using green muscardine fungus and sex attractant synergy prevention and treatment planthopper:
It is realized by following steps:
Step 1: sex pheromone being dissolved in n-hexane and is configured to trapping bait, and is loaded in rubber stopper as lure；The property
Pheromones be 14 carbon triolefin acetic acid esters, tetradecadienyl acetate, 16 carbon triolefin -2- ketone, 16 carbon diene -2- ketone it is mixed
Close object；
Step 2: the lure of step 1 is placed in trapping box, trapping box is put into rice field；
Step 3: by muscardine spore loss in trapping box, trapping planthopper is concentrated in implementation.
Further, in step 1,14 carbon triolefin acetic acid esters, tetradecadienyl acetate, 16 carbon triolefin -2- ketone,
The quality proportioning of 16 carbon diene -2- ketone is (15~18) ︰ (66~75) ︰ (5~8) ︰ (5~8).
Further, in step 1, trapping bait is prepared in the ratio that every 2mg sex pheromone is dissolved in 50ml n-hexane.
Further, in step 1,0.1mg trapping bait is loaded on each rubber stopper.
Further, in step 2, trapping box is cube shaped box body, and the top and bottom of box body are each provided with trapping hole.
Further, in step 2, two lures is set in each trapping box, diagonal positions are suspended on by lifting rope.
Further, in step 2, every 667m2Rice field be arranged 4 trapping boxes.
Further, the time for implementing to concentrate trapping planthopper is planthopper beginning peak period.
Compared with prior art, the present invention has the advantage that
The present invention successfully provides a new method for rice green production.Insect pheromone product is that bionical high-tech produces
Product are discharged artificial synthesized sex pheromone compound by lure, and discharged to field, are lured planthopper to trapper, are used in combination
Disease fungus infects lethal planthopper, reduces insect density, is finally reached prevention and treatment purpose.
A large amount of parasitic prevention and treatment planthoppers are carried out using insect pathogenic bacteria green muscardine fungus to have the advantage that
1, high bioactivity, specificity are strong；2, pest does not develop drug resistance, and does not kill external natural enemy；3, it reduces or removing from
Pesticide use amount is learned, pollution is not constituted to environment, is ideal biological pesticide；4, the chemical pesticide residual in rice product is reduced
Amount improves rice quality；5, using simplicity, expenses for prevention and control is low；6, validity period is long, can continue two months or so drug effects.It is raw
The application of object pesticide and insect pheromone can greatly reduce the use of chemical pesticide, and the residual safety of the agriculture of powerful guarantee grain is reduced
Environmental pollution, the benign cycle for promoting the ecological balance and ecological environment guarantee the sustainable development of China's grain.Therefore in rice
There is vast potential for future development, it is believed that with greatly developing for green agriculture, the application prospect of biological pesticide in the prevention and treatment of pest
It will be more wide.
Detailed description of the invention
Fig. 1 is trapping box structural schematic diagram, 1-cube shaped box body, 2-trapping holes, 3-lifting ropes, 4-lures.
Specific embodiment
In order to make the objectives, technical solutions, and advantages of the present invention clearer, the present invention further uses in detail below
Embodiment and specific test data are illustrated, but the present invention is limited to absolutely not these examples.
Muscardine spore as described below refers both to Metarhizium anisopliae conidium, and the sex pheromone is bio-mimetic syntheses
Object.In present embodiment using insect pathogenic bacteria and insect sex pheromone synergy prevention and treatment planthopper method be by following
Step is realized:
Step 1, by 14 carbon triolefin acetic acid esters, tetradecadienyl acetate, 16 carbon triolefin -2- ketone, 16 carbon two
Alkene -2- ketone, as source is lured, is weighed 2mg and lures source, be then dissolved in 50mL n-hexane and be configured to trap in the mixing of certain mass ratio
Agent, and rubber stopper is loaded in as lure, each lure 0.1mg containing trapping bait；
Step 2: the lure that step 1 obtains is built in trapping box (structure is as shown in Figure 1), trapping box is put into rice field
Above rice seedling at 30cm, by 667m24 trapping boxes are set.
Step 3 wraps up 1x10 with monolayer medical gauze6Spore/g muscardine spore powder, is gently beaten with a small wooden,
Make spore loss in trapping box, the lethal planthopper of infection is concentrated in implementation.
It is to trap and kill adult in beginning peak period that step 3, which implements trapping planthopper adult,.
Using following verification experimental verification invention effects:
1. test material and method
1.1 test site
Place is located at Chongqing City, sand flat town, the Dianjiang County village Bi Qiao academy of agricultural sciences proving ground
1.2 varieties of plant
Rice varieties are Chongqing perfume 203
1.3 planthopper sex pheromone lure cores
Design the lure of four kinds of components:
(1) ten four carbon triolefin acetic acid esters, tetradecadienyl acetate, 16 carbon triolefin -2- ketone, 16 carbon diene -2- ketone
It is to lure source (formula 1) with 18 ︰ of mass ratio, 66 ︰, 8 ︰ 8；
(2) ten four carbon triolefin acetic acid esters, tetradecadienyl acetate, 16 carbon triolefin -2- ketone, 16 carbon diene -2- ketone
It is to lure source (formula 2) with 15 ︰ of mass ratio, 75 ︰, 5 ︰ 5；
(3) ten four carbon triolefin acetic acid esters, tetradecadienyl acetate, 16 carbon triolefin -2- ketone, 16 carbon diene -2- ketone
It is to lure source (formula 3) with 20 ︰ of mass ratio, 60 ︰, 10 ︰ 10；
(4) n-hexane is to lure source (formula 4).
The production method of lure:
(1) it is formulated 1, with above-mentioned 14 carbon triolefin acetic acid esters of planthopper sex pheromone, tetradecadienyl acetate, 16 carbon
Triolefin -2- ketone, 16 carbon diene -2- ketone are the source that lures, and Sigma natural rubber plug is carrier, using preceding different with ethanol washing removal
Taste.18 ︰ of example, 66 ︰, 8 ︰ 8 in mass ratio accurately weighs 2mg sex pheromone mixture, is dissolved in 50ml n-hexane, trapping bait is made, add
Enter rubber stopper and lure, each lure 0.1mg containing trapping bait is made.
(2) 2 are formulated, with the production method of formula 1, the difference is that 15 ︰ of example, 75 ︰, 5 ︰ 5 in mass ratio accurately weighs 2mg letter
Cease plain mixture.
(3) 3 are formulated, with the production method of formula 1, the difference is that 20 ︰ of example, 60 ︰, 10 ︰ 10 in mass ratio accurately weighs 2mg
Pheromones mixture.
(4) 4 are formulated, replaces sex pheromone with n-hexane, Sigma natural rubber plug is carrier, and each lure contains n-hexane
Control treatment lure is made in 0.1mg.
The design of 1.4 trapping boxes
Trapping box is designed to cube shaped, and a length of 40cm of water chestnut is made, upper and lower surface opens circular hole of white PVC board.
The release of 1.5 muscardine spores
1x10 is wrapped up with monolayer medical gauze6Spore/g muscardine spore powder, is gently beaten with a small wooden, spore is made to escape
It dissipates in trapping box.
1.6 test method
1.6.1 influence research of the planthopper sex pheromone formula to green muscardine fungus trapping effect
Four lure formulas, in triplicate, separately setting blank control processing, (i.e. lure does not discharge green as formula 4 for each processing
Stiff bacterium).Trapping box is away from 30cm, every 667m above rice seedling24, surrounding is placed.It institutes an inquiry and records each in trapping box after 3 days
Lethal planthopper quantity is infected in processing, and continuous observation counts 5 days.At the analysis of SPSS19 statistical software and more each formula
Trapping box is managed to the trapping effect of planthopper.
1.6.2 field control effect calculation method
Processing cell and check plot randomly select 5 clumps of rice strains and investigate planthopper adult quantity respectively, calculate insect population decline
Rate and relative control effect.
Revision insect recluced rate (%)=(borer population living after radix-processing before handling)/radix * 100 before handling
Relative control effect (%)=(treatment region Revision insect recluced rate-check plot Revision insect recluced rate)/(check plot 1- insect population decline
Rate) * 100
2. test results and analysis
The test of 2.1 planthopper trapping effects
Green muscardine fungus infects lethal planthopper adult amount in 1 trapping box of table
Note: the different lowercases in table after investigation one columns value of the same day, which represent, has significant difference (P < 0.05), poor
Different significance analysis uses the ANOVA and Tukey of SPSS18.0.
As it can be seen from table 1 formula 1 and formula 2 trap and to infect planthopper effect obvious, counted by SPSS18
Software carries out variance analysis to each processing trapping borer population.The result shows that: one, sex pheromone processing after green muscardine fungus trap and kill planthopper number
Amount is significantly higher than n-hexane control treatment, i.e., without addition sex pheromone, it is less that green muscardine fungus infects planthopper worm amount；Two, it is formulated 1
Borer population is trapped with formula 2 and is higher than formula 3 (the 5th day investigation result show significant differences), and gathering effect has and preferably holds
Effect property.
The test of 2.2 field control effects
By table 2 it can be found that under the attractive effect of sex pheromone, infection lethal effect of the green muscardine fungus to field planthopper
Obvious, formula 1, formula 2,3 treatment region relative control effects of formula are significantly higher than processing control (with the alternative information of n-hexane
Element, 4), the 8th day preventive effect is better than general biological pesticide field efficacy (60% or so) up to 70% or more to formula；It is formulated 1 and matches
2 treatment region Revision insect recluced rates of side and relative control effect are higher than 3 treatment regions of formula.Table of field test results are bright, and the embodiment of the present invention is green
Stiff bacterium and sex attractant composition are preferable to the control effect of planthopper, are significantly better than the preventive effect of green muscardine fungus single dose, appropriate field
Using.
2 different formulations of table processing prevention and treatment planthopper field efficacy
Note: the different lowercases in table after one columns value of relative control effect, which represent, has significant difference (P < 0.05), poor
Different significance analysis uses the ANOVA and Tukey of SPSS18.0.
Claims (8)
1. a kind of method using green muscardine fungus and sex attractant synergy prevention and treatment planthopper, which is characterized in that pass through following step
Suddenly it realizes:
Step 1: sex pheromone being dissolved in n-hexane and is configured to trapping bait, and is loaded in rubber stopper as lure；The property information
Element is the mixing of 14 carbon triolefin acetic acid esters, tetradecadienyl acetate, 16 carbon triolefin -2- ketone, 16 carbon diene -2- ketone
Object；
Step 2: the lure of step 1 is placed in trapping box, trapping box is put into rice field；
Step 3: by muscardine spore loss in trapping box, trapping planthopper is concentrated in implementation.
2. a kind of method using green muscardine fungus and sex attractant synergy prevention and treatment planthopper shown according to claim 1,
It is characterized in that, in step 1,14 carbon triolefin acetic acid esters, tetradecadienyl acetate, 16 carbon triolefin -2- ketone, 16 carbon two
The quality proportioning of alkene -2- ketone is (15~18) ︰ (66~75) ︰ (5~8) ︰ (5~8).
3. a kind of method using green muscardine fungus and sex attractant synergy prevention and treatment planthopper shown according to claim 1,
It is characterized in that, in step 1, trapping bait is prepared in the ratio that every 2mg sex pheromone is dissolved in 50ml n-hexane.
4. a kind of method using green muscardine fungus and sex attractant synergy prevention and treatment planthopper shown according to claim 1,
It is characterized in that, in step 1,0.1mg trapping bait is loaded on each rubber stopper.
5. a kind of method using green muscardine fungus and sex attractant synergy prevention and treatment planthopper shown according to claim 1,
It is characterized in that, in step 2, trapping box is cube shaped box body, and the top and bottom of box body are each provided with trapping hole.
6. a kind of method using green muscardine fungus and sex attractant synergy prevention and treatment planthopper shown according to claim 1,
It is characterized in that, in step 2, two lures is set in each trapping box, diagonal positions are suspended on by lifting rope.
7. a kind of method using green muscardine fungus and sex attractant synergy prevention and treatment planthopper shown according to claim 1,
It is characterized in that, in step 2, every 667m2Rice field be arranged 4 trapping boxes.
8. a kind of method using green muscardine fungus and sex attractant synergy prevention and treatment planthopper shown according to claim 1,
It is characterized in that, implementing to concentrate the time of trapping planthopper is planthopper beginning peak period.
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Cited By (1)
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| CN111903618A (en) * | 2020-08-11 | 2020-11-10 | 福建省农业科学院水稻研究所 | Artificial cultivation method of Anagrus nilaparvatae (Anagrus nilaparvatae) |
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| CN109526987B (en) | 2021-11-26 |
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