CN111903618A - Artificial cultivation method of Anagrus nilaparvatae (Anagrus nilaparvatae) - Google Patents
Artificial cultivation method of Anagrus nilaparvatae (Anagrus nilaparvatae) Download PDFInfo
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- CN111903618A CN111903618A CN202010802716.9A CN202010802716A CN111903618A CN 111903618 A CN111903618 A CN 111903618A CN 202010802716 A CN202010802716 A CN 202010802716A CN 111903618 A CN111903618 A CN 111903618A
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- 238000012364 cultivation method Methods 0.000 title description 2
- 241001556089 Nilaparvata lugens Species 0.000 claims abstract description 74
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- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 70
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- CBFCDTFDPHXCNY-UHFFFAOYSA-N icosane Chemical compound CCCCCCCCCCCCCCCCCCCC CBFCDTFDPHXCNY-UHFFFAOYSA-N 0.000 claims abstract description 46
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The embodiment of the invention discloses an artificial breeding method of Anagrus nilaparvatae (Anagrus nilaparvatae), belonging to the technical field of insect breeding. The method takes the shredded cyperus rotundus or shortleaf kyllinga treated by the eicosane as the egg laying substrate of the brown planthopper, and concretely comprises the following steps: preparing rice seedlings; feeding brown planthoppers; preparing a brown planthopper oviposition substrate; allowing brown planthoppers to lay eggs on an egg laying substrate; parasitic rice louse thysanodes; and (4) releasing in the field. The cyperus rotundus or shortleaf kyllinga herb in the invention passes through 4.0 x 10‑5After the spraying treatment of the eicosane solution with mol/mL, the egg laying amount of brown planthopper on the flower stems of cyperus rotundus and shortleaf kyllinga herb is obviously increased. Compared with a rice seedling spawning substrate, the rice seedling spawning substrate is prepared from cyperus rotundus or shortleaf kyllinga cauliflora, and the risk that brown planthoppers are spread to a rice field can be effectively avoided.
Description
Technical Field
The embodiment of the invention relates to the technical field of insect breeding, in particular to an artificial breeding method of Anagrus nilaparvatae (Anagrus nilaparvatae).
Background
Rice planthoppers are insects of the Hemiptera (Hemiptera) planthopper family (Delphacidae), and common species are brown planthoppers (Nilaparvata lugens), Sogatella furcifera (Sogatella furcifera) and Laodelphax striatellus (Laodelphax striatellus), among which brown planthoppers occur and are the most harmful, and the white planthoppers are the second.
The rice planthopper harms rice in that rice juice is absorbed by rice thorns, so that water and nutrients of the rice are lost, and poor growth and even withering of the rice are caused. In addition, the rice planthopper is also a transmission medium of various rice virus diseases, such as rice straw-like bushy stunt, odontoblast stunt, southern rice black-streaked stunt and the like, and causes double damage to rice. The rice planthopper damages rice, which causes a great amount of yield reduction and brings great loss to agricultural production. Therefore, the rice planthopper has been widely recognized as a significant hazard to world food safety.
For a long time, chemical pesticides are mainly used for preventing and controlling rice planthoppers, but long-term use of the chemical pesticides can cause environmental pollution, destroy ecological balance and continuously enhance drug resistance or drug resistance of pests.
The use of natural enemies in rice fields has been recognized as an effective measure for controlling rice planthoppers. The rice planthopper Thyscrata apis belongs to Anagrus (Anagrus Haliday) and is an important parasitic natural enemy of the rice planthopper at the egg stage, and has a remarkable control effect on the natural population of the rice planthopper. However, the field population quantity of the rice planthopper thysanoptera has obvious dependence and hysteresis on the field population quantity of the rice planthopper, and particularly, the natural population quantity of the rice planthopper thysanoptera is seriously insufficient at the initial stage of outbreak of the rice planthopper, so that the natural population quantity of the rice planthopper thysanoptera is not enough to play an effective control role on the rice planthopper. The natural enemies of the pests are artificially cultured and released in the field, so that the contradiction can be effectively solved.
Disclosure of Invention
Therefore, the embodiment of the invention provides an artificial breeding method of rice planthopper thysanoptera, which can be used for breeding the rice planthopper thysanoptera in a large scale and effectively controlling the population number of the rice planthopper through artificial release before the rice planthopper outbreak.
The embodiment of the invention provides the following technical scheme:
an artificial breeding method of Anagrus nilaparvatae (Anagrus nilaparvatae) uses shredded Cyperus rotundus L or Kyllinga brevifolia Rottb treated by eicosane as oviposition substrates of brown planthoppers.
Specifically, the method comprises the following steps:
1) preparing rice seedlings: planting rice seedlings on a first plastic disc, and transferring the rice seedlings to a first insect cage for feeding brown planthoppers (Nilaparvata lugens) after the rice seedlings grow for 30-40 days;
2) feeding brown planthoppers: feeding brown planthoppers on the rice seedlings in the step 1) in a first insect feeding cage under the conditions that the temperature is 26-28 ℃, the humidity is 60-80%, and the sunlight illumination or LED lamp illumination period L: D is 14: 10;
3) preparation of egg laying substrate of brown planthopper: sowing Cyperus rotundus L or Kyllinga brevifolia Rottb seeds in a second plastic tray, growing at 26-28 deg.C and humidity of 60-80% in sunlight or LED lamp with a period L: D: 14:10, bolting, and using the seeds at a concentration of 4.0 × 10-5The eicosane solution is sprayed and treated to be used as a spawning substrate of brown planthoppers, and a second plastic plate is transferred into a second insect cage for standby;
4) transferring the brown planthoppers in the first insect cage to a new rice seedling for mating and feeding on the same day after eclosion, sucking out the brown planthoppers by using a pest absorber after 5-6 days, transferring the brown planthoppers to the second insect cage, laying eggs on a brown planthopper egg laying substrate, completely absorbing and removing adult brown planthoppers by using the pest absorber after 3-4 days, and then placing the bee species of the rice planthopper tassel wasps with the eclosion rate of 60-80% in the second insect cage for free parasitism;
5) field release: and (3) cutting off the flower stems of the small cyperus rotundus or the shortleaf kyllinga carrying the small rice lice flies from the base parts after 6 days of parasitism, and uniformly cutting in the rice field.
Wherein, eicosane (C)20H42) The preparation method of the solution comprises the following steps: the eicosane crystals were dissolved with acetone and then diluted with distilled water to the desired concentration.
The embodiment of the invention has the following advantages:
1. under the condition without selection, brown planthopper can lay eggs on the shredded cyperus rotundus and the shortleaf kyllinga herb, but the egg laying amount is less, and the shortleaf kyllinga herb and the shredded cyperus rotundus pass through 4.0 multiplied by 10-5After the spraying treatment of the eicosane solution with mol/mL, the egg laying amount of brown planthopper on the flower stems of cyperus rotundus and shortleaf kyllinga herb is obviously increased.
2. Because the cyperus rotundus and the shortleaf kyllinga are not natural hosts of the brown planthopper, when the cyperus rotundus or the shortleaf kyllinga are used as a spawning substrate of the brown planthopper, eggs which are not parasitized by the small rice-louse larvae die the next day after the hatched nymphs do not feed or feed, and only the small rice-louse larvae but not living brown planthoppers are carried on the cyperus rotundus or the shortleaf kyllinga spawning substrate after the parasitized small rice-louse larvae develop to the eclosion stage. Compared with the rice seedling spawning substrate, the cyperus rotundus or shortleaf kyllinga spawning substrate can effectively avoid the risk of brown planthopper spreading to the rice field.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below. It should be apparent that the drawings in the following description are merely exemplary, and that other embodiments can be derived from the drawings provided by those of ordinary skill in the art without inventive effort.
FIG. 1 is a graph showing egg laying scars of brown planthopper on the scape of a shredded cyperus esculentus according to an example of the present invention;
FIG. 2 shows the egg laying trace of brown planthopper on the stems of Kyllinga brevifolia flowers according to the example of the present invention;
FIG. 3 is a cut-stem of Kyllinga brevifolia according to an embodiment of the present invention;
FIG. 4 shows an unstained eggs of Felidae nilotica;
fig. 5 shows the eggs of the brown planthopper infested by the small thysanoptera of the rice lice, which have emerged but not emerged.
Detailed Description
The present invention is described in terms of particular embodiments, other advantages and features of the invention will become apparent to those skilled in the art from the following disclosure, and it is to be understood that the described embodiments are merely exemplary of the invention and that it is not intended to limit the invention to the particular embodiments disclosed. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
An artificial breeding method of Anagus nilaparvatae (Anagus nilaparvatae) comprises the following steps:
1) preparing rice seedlings: planting rice seedlings by using a first plastic disc, transferring the rice seedlings to a first insect cage after growing for 40 days for feeding brown planthoppers (Nilaparvata lugens);
2) feeding brown planthoppers: feeding brown planthoppers on the rice seedlings in the step 1) in a first insect feeding cage under the conditions that the temperature is 26-28 ℃, the humidity is 70%, and the sunlight illumination or LED light illumination period L: D is 14: 10;
3) preparation of egg laying substrate of brown planthopper: sowing Cyperus rotundus L or Kyllinga brevifolia Rottb seeds in a second plastic tray, growing at 26-28 deg.C and 70% humidity under sunlight or LED lamp light period L: D: 14:10, bolting, and using the seeds at concentration of 4.0 × 10-5Eicosane (C)20H42) The solution is sprayed to serve as a oviposition substrate of brown planthoppers, and a second plastic plate is transferred into a second insect cage for standby application, wherein the preparation method of the eicosane solution comprises the following steps: dissolving eicosane crystals by using acetone, and then diluting the dissolved eicosane crystals to the required concentration by using distilled water;
4) transferring the nilaparvata lugens in the first insect rearing cage to a new rice seedling for mating and feeding on the same day after eclosion, sucking out the nilaparvata lugens after 5 days and transferring the nilaparvata lugens to a second insect rearing cage, laying eggs on a oviposition substrate of the nilaparvata lugens, completely sucking and removing adult nilaparvata lugens after 3 days by using an insect sucking device, and then placing the nilaparvata lugens bee seeds with the eclosion rate of 70% in the second insect rearing cage for free parasitism;
5) field release: and (3) cutting off the flower stems of the small cyperus rotundus or the shortleaf kyllinga carrying the small rice lice flies from the base parts after 6 days of parasitism, and uniformly cutting in the rice field.
In view of the risk of insect pest transmission when rice is directly used as a spawning substrate of brown planthoppers, a spawning substrate which can spawn a large amount of eggs on the brown planthoppers and can avoid the transmission of the brown planthoppers needs to be found. The experiment screens various materials, optimizes the screened spawning matrix and improves the spawning amount on the matrix.
Test example 1
The test is carried out in a phytotron in the institute of paddy rice, academy of agricultural sciences of Fujian province, the specifications of the insect-raising cage are 60cm, 40cm and 40cm in length, width and height respectively, and the aperture of the nylon mesh is 100 meshes. The brown planthopper has been artificially propagated for a plurality of generations by using a rice variety TN1, and has consistent and stable biological learning. The screening principle of the oviposition substrate comprises the following steps: 1. the spawning substrate does not cause harm to the paddy field, and 2, brown planthopper can spawn on the spawning substrate. According to the first point of the screening principle, winter gramineous weeds (which die and die in summer and do not cause damage to paddy fields) and terrestrial cyperaceae weeds (which do not cause weed growth in the habitat of paddy fields) are selected as spawning substrates, and the classification, the genus name and the growth habit of various weeds are shown in table 1.
TABLE 1
Growing club grass, beckmannia syzigachne, alopecurus amurensis and bluegrass for 40 days, growing the cyperus rotundus and the shortleaf kyllinga herb until bolting, keeping 100 flowers and stems per disc, and cutting the rest. The experiment was run in a random arrangement with three replicates per treatment. 3 dishes of each oviposition substrate are planted, and 1 dish of each insect cage is planted. The brown planthopper continues to feed on new rice seedlings for 5 days after eclosion mating, the brown planthopper is in a pregnant egg state, the brown planthopper is sucked out by a pest absorber and transferred to a spawning substrate, and 40 female pregnant egg adults are released by each insect cage. After 3 days of spawning, the spawning amount is checked by a stereoscopic microscope.
The results show that: the egg laying amount of brown planthopper on the swamp grass, beckmannia syzigachne, alopecurus amurensis and bluegrass is 0. The eggs of the brown planthopper are detected on the flower stalks of the cyperus rotundus and the shortleaf kyllinga, the average egg laying amount on the cyperus rotundus is 1.6 per root, the average egg laying amount on the shortleaf kyllinga is 1.9 per root, and the two have no obvious difference. Therefore, the invention selects the cyperus rotundus and the shortleaf kyllinga as the egg laying substrate of the brown planthopper.
Test example 2
Earlier experiments show that nonadecane and eicosane have attraction effect on brown planthoppers, and the attraction effect of eicosane is better, so that the concentration of eicosane is compared. Experimental device 4.0X 10-6、4.0×10-5And 4.0X 10-4Three concentrations, three replicates for each concentration, were compared with distilled water. Eicosane is dissolved by acetone and then prepared into the required concentration by distilled water. Crushed cyperus esculentus and shortleaf kyllinga herb are both sucked up, 1 dish is placed in each insect cage, and 50 scapes are left in each dish of spawning substrate. The brown planthoppers continue to feed on new rice seedlings for 5 days after eclosion mating, the rice seedlings are in a white-egg state, and 100 brown planthoppers are inoculated into each insect feeding cage. Spraying the oviposition substrate with the three concentrations of the eicosane solution before inoculating the brown planthopper, inoculating the brown planthopper after the eicosane solution is dried (about 3 hours), cutting off the flower stem from the base part for microscopic examination after 3 days of oviposition, and counting the oviposition amount. The results are shown in Table 2.
TABLE 2
Note: the egg laying amount refers to the egg laying amount of brown planthopper on the stems of Kyllinga brevifolia/cyperus rotundus
The results show that: the cyperus rotundus and the shortleaf kyllinga are not natural hosts of the brown planthopper, and the brown planthopper can lay eggs on stems of the two types of stalks under the condition of no selection, but the egg quantity is less, and the egg laying quantity is 7.3-8.1 grains per root. But the cyperus rotundus and the shortleaf kyllinga herb pass through 4.0 multiplied by 10-5-4.0×10-4After the treatment of the eicosane solution with mol/mL, the egg laying amount of the brown planthopper reaches 59.6-60.1 grains/root, which is obviously higher than 4.0 multiplied by 10-6A group treated with a mol/mL eicosane solution and a blank control group. In order to reduce the dosage and the production cost, the preferred concentration of the eicosane solution is 4.0X 10-5mol/mL。
Although the invention has been described in detail above with reference to a general description and specific examples, it will be apparent to one skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (3)
1. An artificial breeding method of Anagrus nilaparvatae (Anagrus nilaparvatae) is characterized in that the method takes shredded Cyperus rotundus L or Kyllinga brevifolia Rottb treated by eicosane as an oviposition substrate of brown planthopper.
2. The artificial breeding method of Anagrus nilaparvatae (Anagrus nilaparvatae) according to claim 1, comprising the steps of:
1) preparing rice seedlings: planting rice seedlings on a first plastic disc, and transferring the rice seedlings to a first insect cage for feeding brown planthoppers (Nilaparvata lugens) after the rice seedlings grow for 30-40 days;
2) feeding brown planthoppers: feeding brown planthoppers on the rice seedlings in the step 1) in a first insect feeding cage under the conditions that the temperature is 26-28 ℃, the humidity is 60-80%, and the sunlight illumination or LED lamp illumination period L: D is 14: 10;
3) preparation of egg laying substrate of brown planthopper: sowing Cyperus rotundus L or Kyllinga brevifolia Rottb seeds in a second plastic tray, growing at 26-28 deg.C and humidity of 60-80% in sunlight or LED lamp with a period L: D: 14:10, bolting, and using the seeds at a concentration of 4.0 × 10-5The eicosane solution is sprayed and treated to be used as a spawning substrate of brown planthoppers, and a second plastic plate is transferred into a second insect cage for standby;
4) transferring the brown planthoppers in the first insect cage to a new rice seedling for cross feeding on the same day after eclosion, sucking out the brown planthoppers by using a pest absorber after 5-6 days, transferring the brown planthoppers to the second insect cage, laying eggs on a brown planthopper egg laying substrate, completely absorbing and removing adult brown planthoppers by using the pest absorber after 3-4 days, and then placing the bee species of the rice planthopper tassel wasps with the eclosion rate of 60-80% in the second insect cage for free parasitism;
5) field release: and (3) cutting off the flower stems of the small cyperus rotundus or the shortleaf kyllinga carrying the small rice lice flies from the base parts after 6 days of parasitism, and uniformly cutting in the rice field.
3. The artificial breeding method of Anagrus nilaparvatae (Anagrus nilaparvatae) according to claim 2, wherein the preparation method of the eicosane solution comprises: the eicosane crystals were dissolved with acetone and then diluted with distilled water to the desired concentration.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113349166A (en) * | 2021-07-23 | 2021-09-07 | 福建省农业科学院水稻研究所 | Method for breeding rice louse trichogramma in batches |
| CN115868459A (en) * | 2022-12-16 | 2023-03-31 | 福建省农业科学院水稻研究所 | Application of manila as spawning carrier of small broad-head planthopper in cold storage of small rice-lice tassel bees and cold storage method of small rice-lice tassel bees |
| CN116171941A (en) * | 2023-03-08 | 2023-05-30 | 福建省农业科学院水稻研究所 | Application of wheat seedlings in cold storage of trichogramma longsynanthassemblies and cold storage method |
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| CN116171941A (en) * | 2023-03-08 | 2023-05-30 | 福建省农业科学院水稻研究所 | Application of wheat seedlings in cold storage of trichogramma longsynanthassemblies and cold storage method |
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