CN113349166A - Method for breeding rice louse trichogramma in batches - Google Patents
Method for breeding rice louse trichogramma in batches Download PDFInfo
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- CN113349166A CN113349166A CN202110838990.6A CN202110838990A CN113349166A CN 113349166 A CN113349166 A CN 113349166A CN 202110838990 A CN202110838990 A CN 202110838990A CN 113349166 A CN113349166 A CN 113349166A
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- trichogramma
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- nilotica
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- 235000007164 Oryza sativa Nutrition 0.000 title claims abstract description 87
- 235000009566 rice Nutrition 0.000 title claims abstract description 87
- 241000256618 Trichogramma Species 0.000 title claims abstract description 64
- 238000000034 method Methods 0.000 title claims abstract description 27
- 238000009395 breeding Methods 0.000 title claims abstract description 15
- 230000001488 breeding effect Effects 0.000 title claims abstract description 15
- 240000007594 Oryza sativa Species 0.000 title 1
- 241000209094 Oryza Species 0.000 claims abstract description 88
- 235000013601 eggs Nutrition 0.000 claims abstract description 44
- 241001556089 Nilaparvata lugens Species 0.000 claims abstract description 33
- 210000004681 ovum Anatomy 0.000 claims abstract description 17
- 241000196324 Embryophyta Species 0.000 claims description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- 230000032669 eclosion Effects 0.000 claims description 12
- 239000002689 soil Substances 0.000 claims description 12
- 241000238631 Hexapoda Species 0.000 claims description 9
- 238000012258 culturing Methods 0.000 claims description 6
- 241000256844 Apis mellifera Species 0.000 claims description 4
- 235000013305 food Nutrition 0.000 claims description 4
- 235000012907 honey Nutrition 0.000 claims description 4
- 238000002791 soaking Methods 0.000 claims description 4
- 206010021033 Hypomenorrhoea Diseases 0.000 claims description 3
- 238000005286 illumination Methods 0.000 claims description 3
- 238000011534 incubation Methods 0.000 claims description 2
- 230000008186 parthenogenesis Effects 0.000 claims description 2
- 230000000384 rearing effect Effects 0.000 claims 11
- 230000003071 parasitic effect Effects 0.000 abstract description 4
- 241000607479 Yersinia pestis Species 0.000 abstract description 3
- 238000010923 batch production Methods 0.000 abstract description 2
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 230000008569 process Effects 0.000 abstract description 2
- 241000819999 Nymphes Species 0.000 abstract 1
- 241001498622 Cixius wagneri Species 0.000 description 19
- 230000018109 developmental process Effects 0.000 description 4
- 230000017448 oviposition Effects 0.000 description 4
- 230000013011 mating Effects 0.000 description 3
- 230000024241 parasitism Effects 0.000 description 3
- 241000287828 Gallus gallus Species 0.000 description 2
- 241001674048 Phthiraptera Species 0.000 description 2
- 241000593989 Scardinius erythrophthalmus Species 0.000 description 2
- 241000256856 Vespidae Species 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 201000005111 ocular hyperemia Diseases 0.000 description 2
- 230000033458 reproduction Effects 0.000 description 2
- 230000014639 sexual reproduction Effects 0.000 description 2
- 241001466042 Fulgoromorpha Species 0.000 description 1
- 241001470017 Laodelphax striatella Species 0.000 description 1
- 241000176086 Sogatella furcifera Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 238000002224 dissection Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 230000001617 migratory effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a method for breeding rice louse trichogramma in batches, and particularly relates to the field of biological control of agricultural pests. The method comprises the steps of rice seed seedling culture, nymph emergence and rice louse trichogramma culture. The method utilizes unfertilized eggs of the brown planthopper as the hosts of the trichogramma nilotica lugens, even if the parasitic rate does not reach 100 percent and the eggs of the brown planthopper which are not parasitized appear, the eggs can not develop and hatch nymphs due to unfertilization, and the risk of damage caused by accidental release of the brown planthopper to the rice field is avoided in the process of releasing the trichogramma nilotica lugens in the outdoor rice field. The invention is beneficial to the mass and batch production of the trichogramma nilotica, and realizes the controllability of the number and the emergence period of the trichogramma nilotica; the invention has low cost and simple operation.
Description
Technical Field
The invention relates to the field of biological control of agricultural pests, in particular to a method for breeding rice louse trichogramma in batches.
Background
The rice planthopper (mainly comprising brown planthopper, sogatella furcifera and laodelphax striatellus) becomes one of main pests in China after 50 years in the 20 th century, and when nymphs and adults of the rice planthopper absorb juice of rice plants through piercing-sucking mouthparts, various viruses are transmitted to further damage the rice plants. The rice planthopper has migratory flight property, if the rice planthopper migrates into a rice area in time, the rice planthopper finds and takes control measures, and the rice planthopper can quickly outbreak disasters.
The rice planthopper breeds offspring in a sexual reproduction mode, after female adults complete mating, a crack is marked on the epidermis of a leaf sheath through an ovipositor on the abdomen of the female adults, eggs are laid in the crack one by one, and the number of the eggs is from ten to ten, so that egg strips are formed. If the hens are not mated, the hens can lay eggs, and the female rice planthopper adults can lay eggs under the condition of not mating, and the egg laying quantity is considerable. After rice planthoppers are laid eggs by trichogramma nilotica, such as pseudooligotrichogramma pseudooligogyumatsumatsui, female trichogramma adults can lay trichogramma eggs (including fertilized and unfertilized eggs) into the eggs of the trichogramma by using their own slender egg laying device. The trichogramma ova are incubated in the planthopper ova to form larvae, and then continuously parasitize in the planthopper ova, nutrition is obtained from the planthopper ova tissues and developed until adults are drilled out, the planthopper ova cannot develop and hatch due to damage, and the egg parasitism of the trichogramma of the rice louse can play a good role in preventing and controlling the rice planthopper. Unlike borer moth trichogramma and rice louse trichogramma, the feed has small size, unique parasitic mode and great feeding difficulty. At present, no stable large-scale artificial feeding method exists, so that the application of the rice louse trichogramma is limited.
Disclosure of Invention
Therefore, the invention provides a method for breeding rice louse trichogramma in batches, which solves the problem that the existing trichogramma breeding difficulty is high.
In order to achieve the above object, the present invention provides the following technical solutions:
according to the invention, the method for feeding the trichogramma nilotica in batches comprises the following steps:
s1, uniformly scattering the treated rice seeds on the surface of the treated soil in a black lightproof seedling raising pot; cultivating at room temperature to obtain a rice seedling pot;
step S2, placing the seedling raising pot of the rice seedling plant cultivated in the step S1 into a net cage, placing mated adult brown planthoppers into the net cage, and removing the adult after the female adult produces fertilized eggs on the rice seedling plant; breeding the rice seedlings with the eggs in an insect breeding room, and after the fertilized eggs hatch nymphs, culturing the nymphs to eclosion imagoes;
step S3, placing the seedling raising pot of the rice seedling plant cultivated in the step S1 into a net cage, placing the eclosion female short-wing unmatched brown planthopper imagoes cultivated in the step S2 into the net cage, and removing the imagoes after the unmatched female imagoes produce unfertilized eggs on the rice seedling plant;
and S4, placing the female imagoes of the red-eye wasps without mating and preliminary emergence into the net cage of the step S3, and utilizing the egg laying device to lay the eggs into the unfertilized eggs of the brown planthoppers in the rice seedling strains, namely finishing the batch feeding of the red-eye wasps of the rice lice.
Further, in the step S1, the rice seed is treated by soaking the rice seed in water for 24-48h and then exposing the rice seed to white.
Further, in step S1, the treated soil is treated by soaking the soil in water.
Further, in the step S1, the incubation time at room temperature is 5 to 7 days.
Further, in the step S2, the adult insect removing time is 24 hours later.
Further, in the step S2, the condition that the rice seedlings with eggs are cultivated in the insect breeding chamber is 28 +/-2 ℃; the normal light period is 10-16 hours of the illumination time in the daytime.
Further, in the step S3, the eclosion female adult young broilers are female adult young broilers which are continuously raised for 2-3 days after eclosion.
Further, in the step S3, the adult insect removing time is 48 hours later.
Further, the adult is removed by laying the rice louse trichogramma in a cage for 48 hours, and 10-30% of honey water is provided in the cage for feeding the trichogramma adult to maintain the vitality; after 48 hours the honeybee water was removed and the trichogramma adults died due to lack of food.
The invention has the following advantages:
the method utilizes unfertilized eggs of the brown planthopper as the hosts of the trichogramma nilotica lugens, even if the parasitism rate does not reach 100 percent and the unharasitized eggs of the brown planthopper appear, the eggs can not develop and hatch nymphs due to unfertilization, and the risk of damage caused by accidental release of the brown planthopper to the rice field is avoided in the process of releasing the trichogramma nilotica lugens in the outdoor rice field.
According to the invention, parthenogenesis control of the trichogramma huyasi is utilized for reproduction, compared with sexual reproduction, the reproduction is faster, and the obtained offspring quantity is more.
The invention is beneficial to the mass and batch production of the trichogramma nilotica, and realizes the controllability of the number and the emergence period of the trichogramma nilotica; the invention has low cost and simple operation.
Detailed Description
The embodiments of the present invention are described below with reference to specific embodiments, and other advantages and effects of the present invention will be readily apparent to those skilled in the art from the description herein. It should be apparent that the embodiments described herein are part, not all, of the embodiments of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The trichogramma nilotica of the invention refers to all trichogramma which can parasitize rice planthopper eggs, such as pseudooligotrichogramma pseudooligogypsomatsui of the rice planthopper and the like.
The normal light period is 10-16 hours of the illumination time in the daytime.
Example 1A method for raising Ceratopterus Nipponica in batches
S1, filling soil soaked by water in a black lightproof seedling raising pot, and uniformly scattering exposed rice seeds soaked in water for more than 24 hours (the longest time is not more than 48 hours) on the soil surface; placing at room temperature, culturing for 5 days, wherein the rice seedling plants in the seedling pot grow to have one core and two leaves, the distance from soil is about 10cm, and the rice seedling plants can be used for receiving nits;
s2, placing the seedling raising pots cultivated with the rice seedling plants in the step S1 into a net cage, placing enough female brown planthopper adults which have been mated into the net cage, enabling the female adults to lay fertilized eggs on the rice seedling plants, and removing the adults after 24 hours; continuously culturing the rice seedlings with the eggs in an insect-culturing room at room temperature and in a normal light cycle, after the fertilized eggs hatch nymphs, the nymphs inhabit on the rice seedlings and take the rice seedlings to feed until the nymphs eclose, wherein the eclosion time of the obtained adults is uniform and most of the nymphs eclose on the same day;
step S3, placing the seedling raising pots cultivated with the rice seedling plants by the method of the step S1 into a net cage, placing enough (not less than 20) female short-wing unmatched brown planthopper adults raised in the step S2 and hatched for 2 days into the net cage, and enabling the female adults to lay unfertilized eggs on the rice seedling plants;
and S4, inoculating the brown planthopper female imagoes for 48 hours, then removing all the imagoes in the net cage in the step S3, then putting enough (not less than 10) primary-eclosion rice-hopper trichogramma female imagoes into the net cage in the step S3, and utilizing the egg laying device to lay eggs in unfertilized eggs of the brown planthopper in the rice seedling plant, namely finishing the batch breeding of the rice-hopper trichogramma by utilizing the unfertilized eggs of the brown planthopper.
The method for removing the imagoes is that the rice louse trichogramma lays eggs in a cage for 48 hours, and 10% of honey water is provided in the cage for the trichogramma imagoes to eat so as to maintain the vitality; after 48 hours the honeybee water was removed and the trichogramma adults died due to lack of food.
Through dissection under a stereoscopic microscope, all brown planthopper eggs in the phloem of the rice seedling are carefully taken out by a dissecting needle and placed in a culture dish padded with wet filter paper for continuous cultivation, and the parasitism of the rice planthopper eggs by the rice planthopper trichogramma and the development of the rice planthopper trichogramma in the planthopper eggs are recorded and observed. According to the recorded results, the ratio of the eggs of the nilaparvata lugens parasitized by the trichogramma nilaparvata lugens is 85% -95%, the trichogramma nilaparvata lugens in the eggs of the nilaparvata lugens develops for 5-9 days and then eclosion into imagoes, and the imagoes after eclosion are female.
Example 2 method for raising trichogramma gramineus of rice lice in batches
S1, filling soil soaked by water in a black lightproof seedling raising pot, and uniformly scattering exposed rice seeds soaked in water for 36 hours on the surface of the soil; placing at room temperature, culturing for 6 days, wherein the rice seedling grows to have one core and two leaves, the soil separation height is about 12cm, and the rice seedling can be used for receiving nits;
s2, placing the seedling raising pots cultivated with the rice seedling plants in the step S1 into a net cage, placing enough female brown planthopper adults which have been mated into the net cage, enabling the female adults to lay fertilized eggs on the rice seedling plants, and removing the adults after 24 hours; rice seedlings with eggs are continuously cultivated in an insect breeding room at room temperature and in a normal light cycle, after fertilized eggs hatch nymphs, the nymphs feed on the rice seedlings and inhabit the habitat until the nymphs eclose, the eclosion time of the obtained imagoes is uniform, and most of the imagoes eclosion the same day;
step S3, placing the seedling raising pots cultivated with the rice seedling plants by the method of the step S1 into a net cage, placing enough female short-wing unmatched brown planthopper adults which are bred in the step S2 and are obtained after eclosion for 3 days into the net cage, and enabling the female adults to lay unfertilized eggs on the rice seedling plants;
and S4, inoculating the brown planthopper female imagoes for 48 hours, then removing all imagoes in the net cage in the step S3, then putting enough primary-eclosion rice-hopper trichogramma female imagoes into the net cage in the step S3, and utilizing an ovipositor to lay eggs in unfertilized brown planthoppers in rice seedling plants, namely finishing the batch breeding of the rice-hopper trichogramma by utilizing the unfertilized brown planthoppers.
The method for removing the imagoes is that the rice louse trichogramma lays eggs in a cage for 48 hours, and 10% of honey water is provided in the cage for the trichogramma imagoes to eat so as to maintain the vitality; after 48 hours the honeybee water was removed and the trichogramma adults died due to lack of food.
The treated rice seedlings continue to grow in the net cage, and the adult rice louse trichogramma living bodies can be collected in the net cage after 5-9 days and are all female. Collecting the imagination female imagoes of living rice louse trichogramma, putting the imagoes into a rice seedling net cage containing fertilized eggs and unfertilized eggs of the brown planthopper, dissecting the rice seedlings under a stereoscopic microscope after 48 hours, taking out all the eggs of the brown planthopper, putting the eggs on a culture dish padded with moist filter paper for continuous development, and observing and recording the parasitization of the eggs of the brown planthopper and the development condition of the trichogramma in the eggs. The result shows that the progeny of the rice louse trichogramma bred by the invention still has strong parasitic capacity, normal development condition and parasitic rate maintained above 85%.
Although the invention has been described in detail above with reference to a general description and specific examples, it will be apparent to one skilled in the art that modifications or improvements may be made thereto based on the invention. Therefore, it is intended that all such modifications and improvements within the spirit of the invention be included within the scope of the invention as claimed.
Claims (9)
1. A method for mass rearing of trichogramma nilotica, comprising the steps of:
s1, uniformly scattering the treated rice seeds on the surface of the treated soil in a black lightproof seedling raising pot; cultivating at room temperature to obtain a rice seedling pot;
step S2, placing the seedling raising pot of the rice seedling plant cultivated in the step S1 into a net cage, placing mated adult brown planthoppers into the net cage, and removing the adult after the female adult produces fertilized eggs on the rice seedling plant; breeding the rice seedlings with the eggs in an insect breeding room, and after the fertilized eggs hatch nymphs, culturing the nymphs to eclosion imagoes;
step S3, placing the seedling raising pot of the rice seedling plant cultivated in the step S1 into a net cage, placing the eclosion female short-wing unmatched brown planthopper imagoes cultivated in the step S2 into the net cage, and removing the imagoes after the unmatched female imagoes produce unfertilized eggs on the rice seedling plant;
and S4, placing the female imagoes of the unmatched primary-emergence rice-hopper trichogramma into the net cage of the step S3, enabling the female imagoes to lay eggs in unfertilized eggs of the brown planthoppers in the rice seedling plants in a parthenogenesis mode, and finishing batch breeding of the rice-hopper trichogramma.
2. The method for mass rearing of trichogramma nilotica according to claim 1, wherein the step S1 is a step of exposing the treated rice seeds to white water after soaking the rice seeds in water for 24-48 hours.
3. The method for mass rearing of trichogramma nilotica according to claim 1, wherein the treated soil is treated by soaking the soil thoroughly with water in step S1.
4. The method for mass rearing of trichogramma nilotica according to claim 1, wherein in the step S1, the incubation period at room temperature is 5 to 7 days.
5. The method for mass rearing of trichogramma nilotica according to claim 1, wherein the adult insect removal time in step S2 is 24 hours later.
6. The method for mass rearing of trichogramma nilotica according to claim 1, wherein in the step S2, the condition for rearing the rice seedling with eggs in the insect rearing chamber is 28 ± 2 ℃; the normal light period is 10-16 hours of the illumination time in the daytime.
7. The method for mass rearing of rice louse trichogramma as claimed in claim 1, wherein in said step S3, the hatched female unmated brown planthopper adult is a female unmated brown planthopper adult which is kept for 2-3 days after the emergence.
8. The method for mass rearing of trichogramma nilotica according to claim 1, wherein the adult insect removal time in step S3 is 48 hours later.
9. The method for mass rearing of trichogramma nilotica as claimed in claim 1, wherein the adult larvae are removed by laying the trichogramma nilotica in a cage for 48 hours, and the cage is supplied with 10-30% honey water for feeding by the trichogramma adult larvae to maintain vitality; after 48 hours the honeybee water was removed and the trichogramma adults died due to lack of food.
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| CN202110838990.6A CN113349166B (en) | 2021-07-23 | 2021-07-23 | Method for breeding rice louse trichogramma in batches |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN115868459A (en) * | 2022-12-16 | 2023-03-31 | 福建省农业科学院水稻研究所 | Application of manila as spawning carrier of small broad-head planthopper in cold storage of small rice-lice tassel bees and cold storage method of small rice-lice tassel bees |
| CN116171941A (en) * | 2023-03-08 | 2023-05-30 | 福建省农业科学院水稻研究所 | Application of wheat seedlings in cold storage of trichogramma longsynanthassemblies and cold storage method |
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| ES2064163T3 (en) * | 1990-12-18 | 1995-01-16 | Union Nationale Des Cooperativ | OOFAGO INSECT FEMALE MOBILITY ACTIVATION PROCEDURE. |
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| CN112753658A (en) * | 2021-01-17 | 2021-05-07 | 浙江农林大学 | Method for indoor breeding trichogramma dendrolimi by utilizing bombyx camphorata eggs |
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| CN115868459A (en) * | 2022-12-16 | 2023-03-31 | 福建省农业科学院水稻研究所 | Application of manila as spawning carrier of small broad-head planthopper in cold storage of small rice-lice tassel bees and cold storage method of small rice-lice tassel bees |
| CN116171941A (en) * | 2023-03-08 | 2023-05-30 | 福建省农业科学院水稻研究所 | Application of wheat seedlings in cold storage of trichogramma longsynanthassemblies and cold storage method |
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| CN113349166B (en) | 2022-10-28 |
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