CN109526885B - Expanding propagation method of drone of apis cerana japonicas - Google Patents
Expanding propagation method of drone of apis cerana japonicas Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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Abstract
The invention discloses a propagation expanding method of a Japanese scale aphid vespid drone, which comprises the following steps of (1) processing host plants; (2) cultivating a primary host; (3) cultivating a secondary host; (4) and breeding the drone of the Japanese scale aphid. The method adopts a method for changing a parasitic mode to improve the proportion of male bees, utilizes the cercaria acolybia as a primary host, is favorable for keeping the parasitism of the small Japanese scale aphids on the cercaria acobia, keeps the field control effect, provides the conditions of the optimal interval time between two parasitic times, the optimal host age, the optimal bee-receiving proportion and the like of the small Japanese scale aphids, improves the propagation efficiency of the male bees of the small Japanese scale aphids, can realize batch production and propagation of high-quality small Japanese scale aphids, can effectively solve the problem of low propagation efficiency, and realizes the control of the number of pest populations.
Description
Technical Field
The invention relates to the technical field of prevention and control of agricultural and forestry pests, in particular to a propagation method of drone of a small Japanese scale aphid.
Background
Ericerus pela (Paraasaissetia nigra Nitner) is a multi-host pest, and is described with 36 families of 160 host plants, and since 2004, the large-area outbreak of the insect on Yunnan rubber trees has become an important pest on Yunnan and Henan rubber trees. In order to prevent and control the spreading damage of the Erysipela pelykii, the current main prevention and control method still utilizes organic phosphorus chemical agents such as dimethoate, chlorpyrifos, Alisma and the like to carry out spraying or smoke prevention and control. At present, because the used medicaments belong to middle-low toxicity medicaments and have deviation in effect on scale insects, and because the trees of rubber trees are tall and big, the medicament is difficult to be delivered to the damaged parts of the rubber trees by a spraying method, the smoke rule is harsh under the application conditions and the types of effective medicaments are lack, the overall prevention and treatment is not ideal.
The natural enemy insect is used for preventing and controlling the Erysipela pelykii, which is an ideal choice for continuously preventing and controlling the Erysipelykii pelykii. Therefore, after the rubber Paralecyrus elaeis erupted on the rubber to cause a disaster, the research institute of tropical crop science and the group in Yunnan developed the survey of natural enemy resources and the basic research on utilization of the natural enemy resources, and screened out the field dominant parasitic wasps such as Japanese Lecanicillium japonicum (Cocophagus japonica company), Lecanicillium maculatum (Aneriastus ceraplase), Paralecyrus pelagis kuwana (Metaphysciaestia Zhang and Huang), elegant Piercus pulcheri (Diversinervus elegans Silvestri).
The Japanese Lecanicillium lecanii bee, as a dominant parasitic natural enemy of Ericarpium lecanii, can parasitize 2-instar nymphs, 3-instar nymphs, early adults and brown adults of Ericarpium lecanii, and can play a role in controlling the insects. Since the Japanese Lecanicillium lecanii is an amphiprotic reproduction parasitic wasp, the Japanese Lecanicillium lecanii has the characteristic of producing male in the female larva of the same or different parasitic wasps. When the parasitic wasps bred in 2 days after the inoculation of the wasps are artificially bred in indoor large scale, the phenomenon of extremely deviating from female wasps exists, and the female and male proportion is imbalanced. Researches find that the drone of the small Japanese scale aphid has the habit of mating with different female bees for multiple times, and the repeated mating habit of the drone makes up the shortage of the drone to a certain extent when the drone is propagated indoors in a large scale, so that the great significance is achieved. In order to improve the control effect of the wasps on pests in the field, the expanding propagation method of the drone of the small wasps of the mealybug shall be provided, the proportion of a certain number of the drone is increased, and the effect of effectively controlling the pests is achieved.
Disclosure of Invention
In view of the above, the invention provides a method for expanding propagation of the drone of the icerya purchasi gray, which solves the problems of low proportion, high cost and low parasitic efficiency of the drone and is a method for expanding propagation of the drone of the icerya purchasi gray by the heavy parasitic characteristic of the parasitic wasp.
The technical means adopted by the invention are as follows: a propagation method of drone of Japanese scale aphid comprises the following steps:
(1) treatment of host plants
Selecting pumpkin as host plant, sterilizing the surface of the pumpkin, and drying in the air;
(2) cultivation of Primary hosts
Collecting nymphs hatched initially by Erysipelothrix fargesii, and inoculating the nymphs to the surface of the pumpkin for cultivation until the nymphs grow to 3 days old;
(3) cultivation of Secondary hosts
Selecting mated female wasps of the small Oncorhynchus geckii as mother bees, putting the pumpkin with 3-day old Lecanicillium lecanii nymphs obtained in the step (2) into a bee inoculating cage, inoculating parasitic wasps for the first time, wherein the number ratio of the inoculated parasitic wasps to the Lecanicillium lecanii nymphs is 1: 30-35, continuously culturing the pumpkin inoculated with the bees, and culturing female bee parasitic bee larvae of 2-3 days old for later use;
(4) breeding of drone of Okayama japonica
Selecting mated small Japanese scale aphids as mother bees, placing the pumpkin carrying the secondary hosts into a drone inoculating cage, inoculating parasitic wasps for the second time, inoculating the parasitic wasps for the second time at an interval of 6-10 days after inoculating the parasitic wasps for the first time, wherein the number ratio of the inoculated parasitic wasps to the secondary hosts is 1: 20-25, placing the pumpkin inoculated with the bees into a drone expanding and propagating room for drone breeding, when the pupation rate is 90% -100%, stripping parasitic bee pupae from the surface of the pumpkin, and packaging and storing the pumpkin or using the pumpkin and the female bees for field release.
Further, in the step (3), the temperature of the parasitic wasp inoculated for the first time in the wasp inoculating cage is 25-27 ℃, the photoperiod is L: D: 12h, and the humidity is 70% -80%.
Further, in the step (4), the temperature of the bee cage and the parasitic bees inoculated for the second time in the propagation room is 26-28 ℃, the photoperiod L: D is 12h:12h, the humidity is 70-80%, and a sucrose aqueous solution with the mass fraction of 18-22% is added.
Further, after the parasitic wasps are inoculated for 1.5-2.5 d in the first inoculation, redundant parasitic wasps are removed, and then cultivation is carried out.
Further, redundant parasitic wasps are removed after the parasitic wasps are inoculated for the second time for 1.0-2.5 days.
Further, in the step (4), 25-35% of wild parasitic wasp seeds are added into the drone inoculating cage every 2 months for rejuvenation.
The propagation method for the drone of the Japanese scale aphid has the following beneficial effects that:
1. the method is low in cost and can be completed without adding extra test conditions.
2. The method does not need to waste any resource when the parasitic mode of the Lecharge apis mellifera.
3. The invention provides the conditions of the optimal interval time of two bee-inoculating, the primary host age, the bee-inoculating time and the like for improving the proportion of the small Japanese scale aphids, improves the propagation efficiency of the male bees of the small Japanese scale aphids, and solves the problem that the bees are extremely female during conventional propagation.
4. According to the invention, the rubber paracercus elaeis is directly used as a primary host, and the female bee larva of the Japanese scale aphid apis cerana is used as a secondary host, so that the re-parasitic rate of the Japanese scale aphid apis cerana is improved, and the male proportion and the field control effect of the apis cerana are improved.
Detailed Description
The principles and features of this invention are described below in conjunction with examples which are set forth to illustrate, but are not to be construed to limit the scope of the invention.
Example 1
A propagation method of drone of Japanese scale aphid comprises the following steps:
(1) treatment of host plants
Selecting pumpkin as host plant, preparing the thiophanate methyl wettable powder into liquid medicine by using clear water, spraying the liquid medicine on the surface of the pumpkin to perform disinfection and sterilization treatment, and drying the pumpkin in the air.
(2) Cultivation of Primary hosts
Collecting the nymphs of Lecanicillium acornis which are hatched initially, inoculating the nymphs to the surface of pumpkin for cultivation, wherein the cultivation temperature is 24 ℃, forming a test population taking pumpkin as a host, turning the pumpkin once a day within one week to ensure that the nymphs of Lecanicillium acornis on each surface of the pumpkin are distributed uniformly, and cleaning secretion honeydew regularly in the cultivation process until the nymphs of Lecanicillium acornis which grow to 3 days old are reserved. One part is used for inoculating parasitic wasps, and the other part is used for continuing the development of the parasitic wasps and is reserved as a propagation seed source of the Ericaria arborvitae.
(3) Cultivation of Secondary hosts
Selecting mated female wasps of the small Oncorhynchi apis cerana Gecko as mother bees, putting the pumpkin with 3-day-old nymphs of the Ericerus Gecko in the step (2) into an inoculating bee cage, inoculating parasitic wasps for the first time, wherein the temperature of the inoculating bee cage is 25 ℃, the photoperiod is L: D: 12h, the humidity is 70%, and the quantity ratio of the inoculated parasitic wasps to the nymphs of the Ericerus Gecko is 1:30, removing redundant female parasitic wasps after 1.5 days of wasp inoculation, continuously culturing the pumpkin inoculated with the wasps, and culturing the parasitic wasp larvae of the female wasps with the age of 2-3 days for later use.
(4) Breeding of drone of Okayama japonica
Selecting mated small Japanese scale aphids as mother bees, placing pumpkin carrying secondary hosts into a drone inoculating cage, inoculating parasitic wasps for the second time, wherein the temperature of the drone inoculating cage is 26 ℃, the photoperiod L: D is 12h:12h, the humidity is 70%, adding 18% of sucrose aqueous solution for supplementing nutrition, inoculating the parasitic wasps for the second time at an interval of 6D after inoculating the parasitic wasps for the first time, and the quantity ratio of the inoculated parasitic wasps to the secondary hosts is 1:20, removing redundant female parasitic wasps after 1.0d of wasp inoculation, placing the pumpkin inoculated with the wasps into a male wasp expanding and propagating room for male wasp breeding, peeling parasitic wasp pupae from the surface of the pumpkin by a blade when the pupation rate is 90% in microscopic development progress, packaging and storing or directly using the pumpkin and the female wasps for field release, and adding 25% of wild parasitic wasp bee species into a male wasp inoculation cage every 2 months for rejuvenation.
Example 2
A propagation method of drone of Japanese scale aphid comprises the following steps:
(1) treatment of host plants
Selecting pumpkin as host plant, preparing the thiophanate methyl wettable powder into liquid medicine by using clear water, spraying the liquid medicine on the surface of the pumpkin to perform disinfection and sterilization treatment, and drying the pumpkin in the air.
(2) Cultivation of Primary hosts
Collecting the nymphs of Lecanicillium acornis which are hatched initially, inoculating the nymphs to the surface of pumpkin for cultivation, wherein the cultivation temperature is 26 ℃, forming a test population taking pumpkin as a host, turning the pumpkin once a day within one week to ensure that the nymphs of Lecanicillium acornis on each surface of the pumpkin are distributed uniformly, and cleaning secretion honeydew regularly in the cultivation process until the nymphs of Lecanicillium acornis which grow to 3 days old are reserved. One part is used for inoculating parasitic wasps, and the other part is used for continuing the development of the parasitic wasps and is reserved as a propagation seed source of the Ericaria arborvitae.
(3) Cultivation of Secondary hosts
Selecting mated female wasps of the small Oncorhynchi apis cerana Gecko as mother bees, putting the pumpkin with 3-day-old nymphs of the Ericerus Gecko in the step (2) into an inoculating bee cage, inoculating parasitic wasps for the first time, wherein the temperature of the inoculating bee cage is 27 ℃, the photoperiod is L: D: 12h, the humidity is 80%, and the quantity ratio of the inoculated parasitic wasps to the nymphs of the Ericerus Gecko is 1:35, removing redundant female parasitic wasps after 2.5 days of wasp inoculation, continuously culturing the pumpkin inoculated with the wasps at the culturing temperature of 26 ℃, and culturing the parasitic wasp larvae of the female wasps with the age of 2-3 days for later use.
(4) Breeding of drone of Okayama japonica
Selecting mated small Japanese scale aphids as mother bees, placing the pumpkin carrying the secondary host into a male bee inoculating cage, inoculating parasitic bees for the second time, wherein the inoculating cage temperature is 28 ℃, and the photoperiod L: d is 12h:12 hours, the humidity is 80 percent, adding 22% sucrose aqueous solution for supplementing nutrition, inoculating parasitic wasps for the second time at an interval of 10d after inoculating parasitic wasps for the first time, wherein the quantity ratio of the inoculated parasitic wasps to secondary hosts is 1:25, removing redundant female parasitic wasps after inoculating the wasps for 2.5d, placing the pumpkin inoculated with the wasps into a male wasp propagation room for male wasp cultivation, wherein the cultivation temperature is 28 ℃, the microscopic examination development progress is performed when the pupation rate is 98%, the parasitic bee pupae are peeled from the surface of the pumpkin by a blade, packaged and stored or directly used for field release with female bees, and 35% of wild parasitic bee species are added into a drone inoculating cage every 2 months for rejuvenation.
Example 3
A propagation method of drone of Japanese scale aphid comprises the following steps:
(1) treatment of host plants
Selecting pumpkin as host plant, preparing the thiophanate methyl wettable powder into liquid medicine by using clear water, spraying the liquid medicine on the surface of the pumpkin to perform disinfection and sterilization treatment, and drying the pumpkin in the air.
(2) Cultivation of Primary hosts
Collecting the nymphs of Lecania acori Leoni which are hatched initially, inoculating the nymphs to the surface of pumpkin for cultivation, wherein the cultivation temperature is 25 ℃, forming a test population taking pumpkin as a host, turning the pumpkin once a day within one week to ensure that the nymphs of Lecania acori Leoni on each surface of the pumpkin are uniformly distributed, and regularly cleaning secretion honeydew in the cultivation process until the nymphs of Lecania acori Leoni which grow to 3 days old are reserved. One part is used for inoculating parasitic wasps, and the other part is used for continuing the development of the parasitic wasps and is reserved as a propagation seed source of the Ericaria arborvitae.
(3) Cultivation of Secondary hosts
Selecting mated female wasps of the small Oncorhynchi apis cerana Gecko as mother bees, putting the pumpkin with 3-day-old nymphs of the Ericerus Gecko in the step (2) into an inoculating bee cage, inoculating parasitic wasps for the first time, wherein the temperature of the inoculating bee cage is 26 ℃, the photoperiod is L: D: 12h, the humidity is 75%, and the quantity ratio of the inoculated parasitic wasps to the nymphs of the Ericerus Gecko is 1:32, removing redundant female parasitic wasps after 2d of wasp inoculation, continuously culturing the pumpkin inoculated with the wasps at the culturing temperature of 25 ℃, and culturing the parasitic wasp larvae of the female wasps of 2-3 days old for later use.
(4) Breeding of drone of Okayama japonica
Selecting mated small Japanese scale aphids as mother bees, placing pumpkin carrying secondary hosts (female parasitic wasp larvae of 3 days old in step (2)) into a male bee inoculating cage, inoculating parasitic wasps for the second time, wherein the temperature of the inoculating cage is 27 ℃, the photoperiod L: D: 12h, the humidity is 75%, and 20% of sucrose aqueous solution is added for supplementing nutrition, inoculating parasitic wasps for the second time at an interval of 8D after inoculating parasitic wasps for the first time, and the quantity ratio of the inoculated parasitic wasps to the secondary hosts is 1:22, removing redundant female parasitic wasps after 2d (48h) of wasp inoculation, putting the pumpkin inoculated with the wasps into a male wasp expanding and propagating room for male wasp breeding, wherein the breeding temperature is 27 ℃, when the pupation rate is 95% in the microscopic development process, stripping the parasitic wasp pupae from the surface of the pumpkin by using a blade, packaging and storing or directly using the pumpkin and the female wasps for field release, and adding 30% of wild parasitic wasp species into a male wasp inoculation cage every 2 months for rejuvenation.
To further verify the beneficial effects of the present invention, the following comparative verification tests were performed:
1. influence of primary host age on parasitism and oviposition of Japanese Lecanicillium lecanii
And (3) experimental setting: in order to determine the influence of the primary host age on the parasitism oviposition, 2-instar nymphs of Ericaria arboricoides (about 10 days), 3-instar nymphs (about 15 days), early adults (about 25 days) and brown adults of the coccid are selected as primary hosts, and the weight percentage of the primary hosts is determined according to the weight percentage of female bees: 1, 1:30, inoculating the local bee-inoculating device in a self-made bee-inoculating machine for 48h, and then placing the pumpkin with the coccid in a photoperiod L: D (h) 12: 12. relative humidity (75 +/-5%) and temperature of 27 deg.C, and culturing in artificial climate box at interval of 8d after first inoculation according to the ratio of parasitic wasp to secondary host of 1:22, inoculating the larvae of the coccid and the parasitic wasp for 48h for the second time, and then dissecting the egg laying number in the larvae of the coccid and the parasitic wasp.
TABLE 1 Effect of primary host age on the re-parasitism of Ouiella japonica
As can be seen from Table 1, the influence of the primary host age on the re-parasitism of the Japanese Lecanicillium lecanii is obvious, and the initial parasitism rate is 79.79 percent which is the highest in the early Erysiphe pellucida; the secondary and secondary parasitism rates were 32.21% and 33.50% respectively, with the highest rates for 3-day old Erysiphe peltata.
2. Influence of primary host age on postmortem development and male sex ratio of Japanese scale aphid apis
To further prove that the re-parasitic behavior can improve the male proportion, selecting 2-year nymphs of Ericaria arboricoides (about 10 days), 3-year nymphs (about 15 days), early adults (about 25 days) and brown adults as primary hosts, and selecting the four-year-old coccid of the Ericaria arboricoides as the female hosts according to the weight ratio of the female bees: 1, 1:30, inoculating the local bee-inoculating device in a self-made bee-inoculating machine for 48h, and then placing the pumpkin with the coccid in a photoperiod L: D (h) 12: 12. relative humidity (75 +/-5)%, temperature of 27 ℃ artificial climate box, breeding into bees, after first inoculation, interval 8d according to the quantity ratio of parasitic bees to secondary hosts is 1: the results of the secondary bee inoculation for 48h at 22 are shown in Table 2.
TABLE 2 influence of primary host age on post-parasitism development and sex ratio of Okaya japonica
As can be seen from table 2, the rate of parasitism of the young japanese myzus persicae on 2-instar nymphs and 3-instar nymphs of ceroplastes rubens was good, and the male ratio was high for the 2-instar nymphs and the 3-instar nymphs as primary hosts, with the highest male ratio for the 3-instar nymphs.
3. Influence of interval time of second bee inoculation on re-parasitizing and oviposition of small Japanese Lecanicillium lecanii
The test method comprises the following steps: selecting 3-instar Erythrococcus peltatus as a primary host (selecting insect blocks with consistent size and uniform distribution), and selecting female bees: 1, 1:30, inoculating the local bee-inoculating device in a self-made bee-inoculating machine for 48h, and then placing the pumpkin with the coccid in a photoperiod L: D (h) 12: 12. culturing in artificial climate box with relative humidity (75 + -5)%, and temperature of 27 deg.C. The interval time after the first bee receiving is 2d, 4d, 6d, 8d, 10d and 12d according to the quantity ratio of parasitic bees to secondary hosts is 1:22, inoculating the larvae of the coccid and the parasitic wasp for 48h for the second time, and then dissecting the egg laying number in the larvae of the coccid and the parasitic wasp. The results are shown in Table 3.
Table 3: influence of interval time of second bee inoculation on re-parasitizing and oviposition of small Japanese Lecanicillium lecanii
As can be seen from Table 3, the secondary parasite ratio of the Oncorhynchus japonicus Temminck.sp decreases with the increase of the interval time within the interval time of 6d to 10d, the re-parasite ratio is in the trend of increasing first and then decreasing, and the re-parasite ratio is the highest at the interval of 8d, and is 32.28%.
4. Influence of different bee inoculation intervals on development of small Japanese Lecanicillium lecanii
To further confirm that the proportion of males can be improved by the re-parasitic behavior, 3-instar Erythrophorus pelykii is selected as a primary host (insect blocks with consistent size and uniform distribution are selected), and the ratio of the male host to the female host is determined according to the following formula: 1, 1:30, inoculating the local bee-inoculating device in a self-made bee-inoculating machine for 48h, and then placing the pumpkin with the coccid in a photoperiod L: D (h) 12: 12. relative humidity (75 +/-5)%, temperature of 27 ℃ artificial climate box, the interval time after first bee receiving is 2d, 4d, 6d, 8d, 10d, 12d according to the quantity ratio of parasitic bee to secondary host is 1: the result of the secondary bee inoculation for 48h in 22 is shown in Table 4.
TABLE 4 Effect of different inter-bee inoculation times on the development of the Oncorhynchus japonicus Temminck
As can be seen from Table 4, the male sex ratio is increased and then decreased at intervals of 6-10 days, the male sex ratio is highest at 8 days, the male sex ratio is decreased after 10 days, the male sex ratio is beneficial to the propagation of the drone at intervals of 8 days, and the male sex ratio reaches 20.1%.
5. Influence of temperature on parasitism and oviposition of small Japanese Lecanicillium lecanii
In order to clarify the influence of temperature on the parasitism spawning, the temperature gradient is set to be 18 ℃, 21 ℃, 24 ℃, 27 ℃ and 30 ℃, the interval time of the second bee inoculation is set to be 8d, the first bee inoculation, the second bee inoculation and the first bee inoculation primary host cultivation are all carried out in the artificial climate box, the first bee inoculation ratio is 1:30, and the second bee inoculation ratio is 1: 22.
TABLE 5 influence of temperature on the re-parasitism of the Oncorhynchus japonicus Temminck & Aphidae apis oviposition
Table 5 shows that the primary parasitic rate increases with the increase in temperature, and the secondary parasitic rate is 52.54% at maximum at 21 ℃, whereas no heavy parasitic phenomenon occurs at 18 ℃, and the heavy parasitic rate is 30.80% at 30 ℃, but the male rate is low at 30 ℃, and 30 ℃ is not used as the optimum temperature for secondary bee-taking, and 27 ℃ is used as the optimum temperature.
6. Influence of temperature on growth and sex ratio of postmortem of Japanese Lecanicillium lecanii
To clarify the effect of temperature on the development of parasitism, bees were inoculated according to the method of test 5 (effect of different temperatures on parasitism of the young Japanese icerya cupreuteri eggs), and then placed in a photoperiod L: D (h) -12: 12. the results of the growth history and male ratio obtained by growing bees in a climate box having a relative humidity of 75. + -.5% and a temperature of 18 ℃ to 21 ℃ to 24 ℃ to 27 ℃ to 30 ℃ are shown in Table 6.
TABLE 6 influence of temperature on growth and sex ratio of the Okaya japonica after parasite infestation
As is clear from Table 6, the proportion of males was the highest at 27 ℃.
7. Influence of different bee inoculation time periods on parasitism and oviposition of small Japanese Lecanicillium lecanii
The experimental method comprises the following steps: in order to clarify the influence of the bee inoculation time on the parasitism spawning, after the second bee inoculation interval time is set to be 8d, the second bee inoculation time is respectively 12h, 24h, 36h, 48h, 52h and 60 h. According to the quantity ratio of parasitic wasps to secondary hosts being 1:22, inoculating.
TABLE 7 influence of different bee inoculation durations on the re-parasitism of the Okaya japonica
As can be seen from Table 7, the rate of parasitism of the Okayama japonica is gradually increased and decreased within 12-60 hours as the time for inoculating the second time is increased, and the rate of parasitism is optimal at 48 hours.
8. Influence of first inoculation quantity proportion on re-parasitizing and oviposition of small Japanese Lecharge aphid
The experimental method comprises the following steps: in order to clarify the influence of the inoculation number of the secondary hosts on the parasitism oviposition, the nymphs of Lecanicillium abruptum with the age of 3 days were used as the secondary hosts, and the ratio of female bees: the scale of the scale insects is 1:28, 1:30, 1:32, 1:35 and 1:38, the bees are inscribed in a homemade local bee-inoculating device for 48h, and then the pumpkin with the scale insects is placed in a photoperiod L: D (h) 12: 12. culturing in artificial climate box with relative humidity of 75 + -5% and temperature of 27 deg.C, inoculating for 48h at an interval of 8d after inoculating bee for the first time, and dissecting egg laying amount in vivo of scale insect and parasitic bee larva.
TABLE 8 influence of the proportion of the number of first inoculations on the re-parasitism of the Oncorhynchus japonicus Temminck & Schlegel oviposition
Table 8 shows that the first inoculation ratio is 1:30 to 1:35, and that the number of the first inoculation ratio is high, and that the number of the second inoculation ratio is high.
9. Influence of the second inoculation quantity ratio on the re-parasitism and egg laying of the small Japanese Lecanicillium lecanii
The experimental method comprises the following steps: in order to determine the influence of the inoculation number of the female wasps and the secondary hosts of the small Japanese gecko aphids on the parasitism oviposition, the secondary hosts obtained in the step (3) are inoculated according to the ratio of the female wasps: secondary hosts 1:18, 1:20, 1: 22. 1:25 and 1:28, inoculating bees in a homemade local bee-inoculating device for 48h, and then placing the pumpkin with the scale insects in a photoperiod L: D (h) 12: 12. culturing in artificial climate box with relative humidity of 75 + -5% and temperature of 27 deg.C, inoculating for 24 hr at an interval of 8d after inoculating bee for the first time, and dissecting egg laying amount in vivo of scale insect and parasitic bee larva.
TABLE 9 Effect of the second inoculation number ratio on the re-parasitism of Ouiea japonica
As is clear from Table 9, the ratio of the second inoculation is 1:20 to 1:25, the parasitism rate is high, and the ratio of the second inoculation is 1:22, the parasitism rate is the highest.
In conclusion, by adopting the propagation method for the drone of the small Japanese scale aphids, which is provided by the invention, the proportion of the drone is improved by changing a parasitic mode, the parasitism of the small Japanese scale aphids on the small Japanese scale aphids is favorably maintained by using the small Japanese scale wax beetles as a primary host, the field control effect is maintained, the conditions of the optimal interval time between two parasitizations of the small Japanese scale aphids, the optimal host age, the bee receiving proportion and the like are provided, the propagation efficiency of the male bee of the small Japanese scale aphids is improved, the high-quality small Japanese scale aphids can be produced and propagated in batch, the problem of low propagation efficiency can be effectively solved, and the control of the number of pest populations is realized.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (6)
1. A propagation expanding method for the drone of the Japanese scale aphid is characterized by comprising the following steps:
(1) treatment of host plants
Selecting pumpkin as host plant, sterilizing the surface of the pumpkin, and drying in the air;
(2) cultivation of Primary hosts
Collecting nymphs hatched initially by Erysipelothrix fargesii, and inoculating the nymphs to the surface of the pumpkin for cultivation until the nymphs grow to 3 days old;
(3) cultivation of Secondary hosts
Selecting mated female wasps of the small Oncorhynchus geckii as mother bees, putting the pumpkin with 3-day old nymphaea acornis in the step (2) into a bee inoculating cage, inoculating parasitic wasps for the first time, wherein the number ratio of the inoculated parasitic wasps to the nymphaea acornis is 1: 30-35, continuously culturing the pumpkin inoculated with the bees, and culturing female bee parasitic bee larvae of 2-3 days old for later use;
(4) breeding of drone of Okayama japonica
Selecting mated small Japanese scale aphids as mother bees, placing the pumpkin carrying the secondary hosts into a drone inoculating cage, inoculating parasitic wasps for the second time, inoculating the parasitic wasps for the second time at an interval of 6-10 days after inoculating the parasitic wasps for the first time, wherein the number ratio of the inoculated parasitic wasps to the secondary hosts is 1: 20-25, placing the pumpkin inoculated with the bees into a drone expanding and propagating room for drone breeding, when the pupation rate is 90% -100%, stripping parasitic bee pupae from the surface of the pumpkin, and packaging and storing the pumpkin or using the pumpkin and the female bees for field release.
2. The expanding propagation method of the drone of the icerya purchasi aphid in Japan according to claim 1, wherein the temperature of the first inoculation of the parasitic wasps in the bee cage in the step (3) is 25-27 ℃, the photoperiod is L: D: 12h, and the humidity is 70-80%.
3. The propagation method of the drone of the icerya purchasi aphid in the step (4) is characterized in that the temperature of the drone cage and the drone inoculated for the second time in the propagation room is 26-28 ℃, the photoperiod L: D: 12h, the humidity is 70-80%, and a sucrose aqueous solution with the mass fraction of 18-22% is added.
4. The propagation method of the drone of the icerya purchasi aphid in Japan according to claim 1, wherein the parasitic wasps are removed after the parasitic wasps are inoculated for 1.5-2.5 days for the first inoculation, and then the drone is cultivated.
5. The propagation method for the drone of the icerya purchasi aphides in Japan according to claim 1, wherein the redundant parasitic wasps are removed after the parasitic wasps are inoculated for 1.0-2.5 days after the parasitic wasps are inoculated for the second time.
6. The propagation method for the drone of the icerya purchasi aphid in Japan according to claim 1, wherein in the step (4), 25 to 35 percent of wild parasitic wasp species are added into the drone inoculating cage every 2 months for rejuvenation.
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