CN102172231A - Method for overwintering species conservation propagation of myzus persicae - Google Patents
Method for overwintering species conservation propagation of myzus persicae Download PDFInfo
- Publication number
- CN102172231A CN102172231A CN 201110024746 CN201110024746A CN102172231A CN 102172231 A CN102172231 A CN 102172231A CN 201110024746 CN201110024746 CN 201110024746 CN 201110024746 A CN201110024746 A CN 201110024746A CN 102172231 A CN102172231 A CN 102172231A
- Authority
- CN
- China
- Prior art keywords
- cigarette
- aphid
- seedling
- cigarette aphid
- meters
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Abstract
The invention provides a method for overwintering species conservation propagation of myzus persicae and belongs to the technical field of prevention and control of aphidius gifuensis. Aiming to solve the problem that the overwintering species conservation of the myzus persicae cannot be realized so that the huge demand for the aphidius gifuensis in the next tobacco planting season is difficultly met in the prior art, the invention provides the method for the overwintering species conservation propagation of the myzus persicae, which comprises the following steps of: a, collecting the myzus persicae; b, inoculating the myzus persicae to tobacco seedlings; c, removing toxins from the myzus persicae; d, purifying and rejuvenating the myzus persicae; e, performing the overwintering species conservation of the myzus persicae; and f, enlarging the propagation of the myzus persicae. The method has the advantages of successfully realizing the large-scale propagation of the myzus persicae, ensuring quality and quantity of the young myzus persicae, providing enough high-quality myzus persicae for the large-scale propagation of the aphidius gifuensis in next year, and creating better ecological benefit, economic benefit and social benefit.
Description
Technical field
The invention belongs to aphidius gifuensis Prevention Technique field, particularly a kind ofly prepare for a large amount of breeding in the back cocoon honeybees that survive the winter, and in the winter time the cigarette aphid is protected kind of a technology of breeding.
Background technology
Tradition mainly depends on chemical control to the control of cigarette aphid, owing to use chemical pesticide for a long time in a large number, the cigarette aphid has produced stronger pesticide resistance, susceptibility to various agricultural chemicals weakens, and causes pesticide dosage to increase and the drug effect reduction, and institute's pesticide grown has produced grievous injury to the various natural enemies of cigarette aphid simultaneously, quantity sharply reduces, along with the increase of pesticide dosage, the residue of pesticide in the tobacco leaf are more and more serious, to the also aggravation day by day of pollution of natural environment.
For addressing the above problem, the biological control technology has appearred at present, promptly prevent and treat the cigarette aphid with aphidius gifuensis, reach the purpose of " controlling worm with worm ", aphidius gifuensis is the natural enemy of cigarette aphid, the cigarette aphid is the host of aphidius gifuensis, aphidius gifuensis can produce ovum in the cigarette aphid body when mate and oviposit, and aphidius gifuensis develops into adult by the internal organ of getting in the food cigarette aphid body, thereby the cigarette aphid is killed, reach the purpose that eliminates destructive insects.
Preventing and treating in the applying of cigarette aphid with aphidius gifuensis, need a large amount of aphidius gifuensis of breeding, promptly carry out the scale breeding of aphidius gifuensis, for this reason, at first to breed the feed of aphidius gifuensis in a large number, it is host's cigarette aphid, yet, scale breeding to the cigarette aphid in the prior art generally is by in the needs control then, gathered the wild tobacco aphid and breed before the tobacco field period, because the time is short, the aphid source is few, the cigarette aphid quantity of breeding out is few, causes the quantity of the aphidius gifuensis that the later stage breeds by parasitic cigarette aphid also less, can not prevent and treat effectively cigarette aphid insect at field period.
Summary of the invention
Can't carry out over wintering to the cigarette aphid for solving in the prior art, be difficult to satisfy the problem of 1 year tobacco planting season, the invention provides a kind of cigarette aphid over wintering mating system, comprise following sequential steps the wilderness demand of aphidius gifuensis:
(a) gather the cigarette aphid:
The annual 8-10 month, select two ages of aptery greatly, no a cocoon honeybee parasitism, the infection of no germ and three ages in the cigarette aphid that will breed the warm canopy as if aphid from the big aptery cigarette aphid of field acquisition with from last one year, wherein, the ratio of the quantity of the cigarette aphid of gathering in the cigarette aphid of field acquisition and the warm canopy is 3: 7;
(b) the cigarette seedling connects aphid:
The cigarette aphid of above-mentioned collection is inoculated on the fresh cigarette seedling of the cultivation in the warm canopy or Turnip Sprouts or the green vegetables seedling breeds, every young plant connects cigarette aphid 8-12 head when connecing aphid, described fresh cigarette seedling be meant emerge back 80 days, do not inoculate the cigarette seedling of cigarette aphid, described fresh Turnip Sprouts or green vegetables seedling be meant emerge back 30 days, do not inoculate Turnip Sprouts or the green vegetables seedling of cigarette aphid;
(c) cigarette aphid detoxification:
Above-mentioned cigarette aphid breeding is after 10 days, reject in spite of illness cigarette strain and cigarette aphid, with anosis cigarette seedling or the cigarette aphid renewed vaccination on the Turnip Sprouts on fresh cigarette seedling or Turnip Sprouts or green vegetables seedling, repeat above-mentioned detoxification process 2-3 time after, filter out the cigarette seedling of stalwartness, virus-free performance and the cigarette aphid on the dish seedling;
(d) cigarette aphid purification and rejuvenation:
The above-mentioned cigarette aphid that filters out is inoculated into fresh cigarette strain bottom leaf in the warm canopy, and every strain connects 20 of cigarette aphids, and described fresh cigarette strain is meant the nine leaves cigarette strain wholeheartedly of being transplanted to behind the warm canopy 25-30 days;
Temperature in the temperature canopy is controlled at 22 ± 5 ℃, and humidity is controlled at 65%-80%, and under the natural lighting condition, breeding cigarette aphid 15-20 days can reach 2000 of individual plant cigarette aphids;
When treating that the cigarette aphid breeds 2000 of every strains, the cigarette aphid is detected, remove host plant in spite of illness, reject in spite of illness the cigarette aphid and by parasitic cigarette aphid, healthy and strong cigarette aphid is cooked the fresh cigarette strain bottom Ye Zaici that generation kind aphid receives in the warm canopy once more and purificates and rejuvenates;
(e) cigarette aphid over wintering:
After late November or early December, cigarette aphid in the above-mentioned temperature canopy is inoculated on the fresh Turnip Sprouts or green vegetables seedling in the glass greenhouse, described fresh Turnip Sprouts or green vegetables seedling are meant emerge back 30 days Turnip Sprouts or green vegetables seedling, every young plant connects cigarette aphid 8-12 head when connecing aphid, in temperature is 22 ± 5 ℃, and humidity is to continue breeding under the condition of 50%-70%;
(f) cigarette aphid expanding propagation:
In late Febuary, second moves into the numerous aphid plant in the above-mentioned glass greenhouse in the warm canopy to by the end of March, breeds in the purification and rejuvenation mode in the step (d).
The present invention is protecting when kind breeding first, described step (a) is gathered in the cigarette aphid, the cigarette aphid of being gathered all is the cigarette aphid of field acquisition, protecting kind since 1 year breeds, above-mentioned while field acquisition is just adopted and from the mode that last one year, warm canopy was gathered in the source of cigarette aphid, and is to gather at 3: 7 by above-mentioned quantitative proportion.
The present invention is by a series of technological means such as above-mentioned selection to guarantor kind of host, purification and rejuvenation, the detoxification of cigarette aphid, child care in winter; successfully realized the scale breeding of cigarette aphid; the quality and the quantity of aphid kind have been guaranteed; for the child care of a large amount of breeding in next year aphidius gifuensis provides sufficient amount and quality cigarette aphid preferably, obtain good ecological, economic benefit and social benefit.
As preferred version of the present invention, described step (d) or (f) in the breeding method of cigarette strain of purification and rejuvenation usefulness comprise following sequential steps:
Grow seedlings the stage: the flue-cured tobacco capsuled seed of K326 or cloud and mist 203 kinds was planted in the seedling-cultivating tray in the cigarette transplantation of seedlings in preceding 70 days, adopts the floating seedlings technology to grow seedlings;
Become the seedling stage: treat the seedling strain Cheng Miaohou in the seedling-cultivating tray, it is transplanted in the basin of putting in warm canopy, the potted plant line-spacing has been 30cm * 30cm, water normal root water while planting, add 0.3kg in every 100kg normal root water and carry seed manure, carrying nitrogenous fertilizer in the seed manure, phosphate fertilizer, potash fertilizer is 12: 6: 24 by weight ratio, and nitrogenous fertilizer is nitrate nitrogen, phosphate fertilizer is phosphorus pentoxide, and potash fertilizer is potassium oxide;
Fertilising in 10 days after the cigarette transplantation of seedlings, converting water 100kg with 100 strains with 300g composite fertilizer and 100-200g urea waters and executes, spray with clear water again, in order to avoid the generation fertilizer damage, nitrogenous fertilizer, phosphate fertilizer, potash fertilizer are 12: 6: 24 by weight ratio in the described composite fertilizer, and nitrogenous fertilizer is ammoniacal nitrogen, phosphate fertilizer is phosphorus pentoxide, potash fertilizer is potassium oxide, later on every fertilising in 10 days once according to growing way, and per 100 strain sealing fertilizer 400-500g;
Water management: the seedling strain is watered sufficient normal root water when transplanting, and accomplishes that later on little water duty waters, and guarantees that basin soil is moistening, the excessive basin soil shallow lake pulpboard knot that causes of avoiding watering;
The regulation and control of temperature, wet, light: the strong fine day of illumination is rolled the film of Wen Peng side, makes warm canopy inner air convection; Launch the sunshade net and reduce illumination, reduce the temperature in the warm canopy; At dusk put down the film of side, make the interior nocturnal temperature of warm canopy be not less than 10 ℃; The cloudy day film of side is not rolled, and packs up the sunshade net simultaneously, and making has suitable illumination in the warm canopy, and temperature is not higher than 25 ℃, and humidity remains on 50%-70%;
The extermination of disease and insect pest: the anthracnose their early stage sprays and it can be killed with 500 times of liquid of 25% carbendazol wettable powder, and the powdery mildew their early stage sprays and it can be killed with 800-1000 times of liquid of 36% thiophanate-methyl suspending agent.
Scheme as a further improvement on the present invention, warm canopy in the such scheme is made of conjoined green house frame and the canopy film that covers the greenhouse frame periphery, comprise two domes, two sides and two end faces, long 50 meters, wide 12 meters of greenhouse frame, 3 meters of shoulder heights, 4.6 meters of sagitta, 3 meters of the column spacings of greenhouse frame, 6 meters of spans, 0.75 meter of spring base, vault is provided with three vertical poles; 4 cover film-coiling devices are arranged on the greenhouse frame, wherein two covers are installed in the greenhouse frame both sides, other two covers are installed in the outer top of greenhouse frame, the greenhouse frame inner top is provided with 60 order sunshade nets, sunshade side off the net is useful on numerous aphid chamber of breeding cigarette aphid, numerous aphid chamber is made of framework and the fly net that covers the framework periphery, there are two rows numerous aphid chamber, arrange along two sides in the greenhouse frame respectively, the numerous aphid of every row chamber has 17, wherein the numerous aphid of row chamber is long 6 meters, wide 3 meters, high 1.8 meters, and another arranges long 4.5 meters, wide 3 meters, high 1.8 meters of numerous aphid chamber, and the numerous aphid of two rows stays 1.5 meters wide aisle between the chamber;
The cigarette basin of planting that the strain of cultivation cigarette is used in the described temperature canopy is a flowerpot, and its diameter is 25.0-30.0cm, and diameter is 15.0-17.0cm at the bottom of the basin, and the basin hem width is that the high flowerpot for 26.0cm of 1.5-2.0cm, basin is advisable as planting the cigarette basin;
The cultivating soil of planting in the cigarette basin is the gutless red soil 1000kg that crosses cigarette and vegetables, add well-rotted farmyard manure 300kg, fused calcium magnesium phosphate 20kg and fully mix thoroughly, heap is sealed the above crude opium of planting of fermentation 30d, adds carbendazim 100g, chlorophos 100g during the dress basin, fully mix thoroughly, prevent soil worm in spite of illness;
When described cigarette aphid inoculates, choose the aphid inoculation with soft little writing brush;
In warm canopy or glass greenhouse, carry out in the cigarette aphid over wintering reproductive process, to prevent and kill off cigarette aphid natural enemy, the natural enemies such as prevention syrphus fly, ladybug, cigarette fleahopper and cocoon honeybee of closing the doors and windows are gone into canopy, and syrphus fly, ladybug, cigarette fleahopper manually caught, to being shifted out outside warm canopy or the glass greenhouse by the aphid kind of cocoon honeybee parasitism;
The high 3.6m of glass greenhouse in the described step (e), wide 5.3m, long 14.2m;
Temperature in the described step (e) adds thermal control by the quartzy warmer of the family expenses of a 1000W, and humidity is controlled with humidifier.
Description of drawings
The present invention is further detailed explanation below in conjunction with the drawings and specific embodiments:
Fig. 1 is the schematic perspective view of warm canopy described in the present invention.
Embodiment
A kind of cigarette aphid over wintering mating system comprises following sequential steps:
(a) gather the cigarette aphid:
In the 8-10 month, select two ages of aptery greatly, no a cocoon honeybee parasitism, the infection of no germ and three ages in the cigarette aphid that will breed the warm canopy as if aphid from the big aptery cigarette aphid of field acquisition with from last one year, wherein, the ratio of the quantity of the cigarette aphid of gathering in the cigarette aphid of field acquisition and the warm canopy is 3: 7, and described two ages are if aphid is the long 0.8-1.0mm of body, the wide 0.4-0.5mm of body, the larva that is pistac, described three ages, the wide 0.6-0.65mm of body was red green or pink larva if aphid is the long 1.2-1.3mm of body;
(b) the cigarette seedling connects aphid:
The cigarette aphid of above-mentioned collection is inoculated on the fresh cigarette seedling of the cultivation in the warm canopy or Turnip Sprouts or the green vegetables seedling breeds, every young plant connects cigarette aphid 8-12 head when connecing aphid, described fresh cigarette seedling be meant emerge back 80 days, do not inoculate the cigarette seedling of cigarette aphid, described fresh Turnip Sprouts or green vegetables seedling be meant emerge back 30 days, do not inoculate Turnip Sprouts or the green vegetables seedling of cigarette aphid;
(c) cigarette aphid detoxification:
Above-mentioned cigarette aphid breeding is after 10 days, reject in spite of illness cigarette strain and cigarette aphid, with anosis cigarette seedling or the cigarette aphid renewed vaccination on the Turnip Sprouts on fresh cigarette seedling or Turnip Sprouts or green vegetables seedling, repeat above-mentioned detoxification process 2-3 time after, filter out the cigarette seedling of stalwartness, virus-free performance and the cigarette aphid on the dish seedling;
The detoxification mechanism of above-mentioned steps: the wild tobacco aphid generally has bushy top virus, arbovirus such as etch virus poison, marmor upsilon, if do not carry out detoxification treatment, cigarette strain behind the inoculation aphid will cause the harm of virus disease, not only influence the breeding amount of cigarette aphid and aphidius gifuensis, increase cost, also can cause the harm of tobacco virus, in order to reduce the propagation that connects host's cigarette strain virus disease behind the aphid, by the above-mentioned steps method, can remove cigarette strain in spite of illness and cigarette aphid, keep and show that not viral cigarette seedling and the cigarette aphid on the dish seedling are used for inoculating again;
(d) cigarette aphid purification and rejuvenation:
The above-mentioned cigarette aphid that filters out is inoculated into fresh cigarette strain bottom leaf in the warm canopy, and every strain connects 20 of cigarette aphids, and described fresh cigarette strain is meant the nine leaves cigarette strain wholeheartedly of being transplanted to behind the warm canopy 25-30 days;
Temperature in the temperature canopy is controlled at 22 ± 5 ℃, and humidity is controlled at 65%-80%, and under the natural lighting condition, breeding cigarette aphid 15-20 days can reach 2000 of individual plant cigarette aphids;
When treating that the cigarette aphid breeds 2000 of every strains, the cigarette aphid is detected, remove host plant in spite of illness, reject in spite of illness the cigarette aphid and by parasitic cigarette aphid, healthy and strong cigarette aphid is cooked the fresh cigarette strain bottom Ye Zaici that generation kind aphid receives in the warm canopy once more and purificates and rejuvenates;
(e) cigarette aphid over wintering:
After late November, cigarette aphid in the above-mentioned temperature canopy is inoculated on the fresh Turnip Sprouts or green vegetables seedling in the glass greenhouse, the high 3.6m of glass greenhouse, wide 5.3m, long 14.2m, described fresh Turnip Sprouts or green vegetables seedling are meant emerge back 30 days Turnip Sprouts or green vegetables seedling, every young plant connects cigarette aphid 8-12 head when connecing aphid, is 22 ± 5 ℃ in temperature, and humidity is to continue breeding under the condition of 50%-70%, temperature adds thermal control by the quartzy warmer of the family expenses of a 1000W, and humidity is controlled with humidifier;
For a long time, the cigarette aphid protects to plant mainly to protect based on the cigarette strain and plants, the cigarette strain is regarded as the host plant of the most suitable cigarette aphid breeding, even survive the winter the population child care stage the cigarette aphid, the cigarette strain also is the main host plants as the cigarette aphid, yet, winter planting is not only time-consuming with cultivation cigarette seedling, and the growth of cigarette strain is slower, the host is single, has not satisfied the needs to detoxification of cigarette aphid and purification and rejuvenation, and the present invention is in the over wintering process, select Turnip Sprouts or green vegetables seedling as the plant that winter, cigarette aphid guarantor planted, can adapt to the cigarette aphid needs that winter, the guarantor planted;
(f) cigarette aphid expanding propagation:
In late Febuary, second moves into the numerous aphid plant in the above-mentioned glass greenhouse in the warm canopy to by the end of March, breeds in the purification and rejuvenation mode in the step (d);
April, finished the first tenday period of a month in May in order to concentrate for aphidius gifuensis multiplication plot kind aphid;
Above-mentioned steps (d) or (f) in the breeding method of cigarette strain of purification and rejuvenation usefulness comprise following sequential steps:
Grow seedlings the stage: the flue-cured tobacco capsuled seed of K326 or cloud and mist 203 kinds was planted in the seedling-cultivating tray in the cigarette transplantation of seedlings in preceding 70 days, adopts the floating seedlings technology to grow seedlings;
Become the seedling stage: treat the seedling strain Cheng Miaohou in the seedling-cultivating tray, it is transplanted in the basin of putting in warm canopy, the potted plant line-spacing has been 30cm * 30cm, water normal root water while planting, add 0.3kg in every 100kg normal root water and carry seed manure, carrying nitrogenous fertilizer in the seed manure, phosphate fertilizer, potash fertilizer is 12: 6: 24 by weight ratio, and nitrogenous fertilizer is nitrate nitrogen, phosphate fertilizer is phosphorus pentoxide, and potash fertilizer is potassium oxide;
Fertilising in 10 days after the cigarette transplantation of seedlings, convert water 100kg with 100 strains with 300g composite fertilizer and 100-200g urea and water and execute, again with the clear water spray, in order to avoid produce fertilizer damage, nitrogenous fertilizer, phosphate fertilizer, potash fertilizer are 12: 6: 24 by weight ratio in the described composite fertilizer, nitrogenous fertilizer is ammoniacal nitrogen, and phosphate fertilizer is phosphorus pentoxide, and potash fertilizer is potassium oxide, later on every fertilising in 10 days once according to growing way, per 100 strain sealing fertilizer 400-500g, later on every fertilising in 10 days once according to growing way, per 100 strain sealing fertilizer 400-500g;
Water management: the seedling strain is watered sufficient normal root water when transplanting, and accomplishes that later on little water duty waters, and guarantees that basin soil is moistening, the excessive basin soil shallow lake pulpboard knot that causes of avoiding watering;
The regulation and control of temperature, wet, light: the strong fine day of illumination is rolled the film of Wen Peng side, makes warm canopy inner air convection; Launch the sunshade net and reduce illumination, reduce the temperature in the warm canopy; At dusk put down the film of side, make the interior nocturnal temperature of warm canopy be not less than 10 ℃; The cloudy day film of side is not rolled, and packs up the sunshade net simultaneously, and making has suitable illumination in the warm canopy, and temperature is not higher than 25 ℃, and humidity remains on 50%-70%;
The extermination of disease and insect pest: the anthracnose their early stage sprays and it can be killed with 500 times of liquid of 25% carbendazol wettable powder, and the powdery mildew their early stage sprays and it can be killed with 800-1000 times of liquid of 36% thiophanate-methyl suspending agent;
Warm canopy in the such scheme as shown in Figure 1, constitute by conjoined green house frame 1 and the canopy film 2 that covers greenhouse frame 1 periphery, comprise two domes, two sides and two end faces, 50 meters, wide 12 meters of greenhouse frame 1 length, 3 meters of shoulder heights, 4.6 meters of sagitta, 3 meters of column 9 spacings of greenhouse frame 1,6 meters of spans, 0.75 meter of spring base, vault is provided with three vertical poles 10; 4 cover film-coiling devices 8 are arranged on the greenhouse frame 1, wherein two covers are installed in greenhouse frame 1 both sides, other two covers are installed in greenhouse frame 1 outer top, greenhouse frame 1 inner top is provided with 60 order sunshade nets 5, sunshade net 5 belows are useful on numerous aphid chamber 6 of breeding cigarette aphid, numerous aphid chamber 6 is made of framework 3 and the fly net 4 that covers framework 3 peripheries, there are two rows numerous aphid chamber 6, arrange along two sides in the greenhouse frame 1 respectively, the numerous aphid of every row chamber 6 has 17, wherein the numerous aphid of row chamber 6 length are 6 meters, wide 3 meters, high 1.8 meters, 90 basin plant are placed in every numerous aphid chamber, can breed 270,000 of cigarette aphids, another arranges 4.5 meters of numerous aphid chamber 6 length, wide 3 meters, high 1.8 meters, 70 basin plant are placed in every numerous aphid chamber, stay 1.5 meters wide aisle 7 between the numerous aphid of the two rows chamber 6, can breed 210,000 of cigarette aphids;
The cigarette basin of planting that the strain of cultivation cigarette is used in the described temperature canopy is a flowerpot, and its diameter is 25.0-30.0cm, and diameter is 15.0-17.0cm at the bottom of the basin, and the basin hem width is that the high flowerpot for 26.0cm of 1.5-2.0cm, basin is advisable as planting the cigarette basin;
The cultivating soil of planting in the cigarette basin is the gutless red soil 1000kg that crosses cigarette and vegetables, add well-rotted farmyard manure 300kg, fused calcium magnesium phosphate 20kg and fully mix thoroughly, heap is sealed the above crude opium of planting of fermentation 30d, adds carbendazim 100g, chlorophos 100g during the dress basin, fully mix thoroughly, prevent soil worm in spite of illness;
When described cigarette aphid inoculates, choose the aphid inoculation with soft little writing brush;
In warm canopy or glass greenhouse, carry out in the cigarette aphid over wintering reproductive process, to prevent and kill off cigarette aphid natural enemy, the natural enemies such as prevention syrphus fly, ladybug, cigarette fleahopper and cocoon honeybee of closing the doors and windows are gone into canopy, and syrphus fly, ladybug, cigarette fleahopper manually caught, to being shifted out outside warm canopy or the glass greenhouse by the aphid kind of cocoon honeybee parasitism.
Since nineteen ninety-eight, Yunnan Province's Yuxi tobacco company employing artificial fecundation release aphidius gifuensis control vega important pests---the cigarette aphid has been obtained good result.According to statistics, the area of Yuxi release aphidius gifuensis control cigarette aphid in 2009 reaches 40.35 ten thousand mu, covers more than 60% of the total vega area in the whole city, and the area that accumulative total discharges aphidius gifuensis control cigarette aphid reaches 1,760,000 mu, reduce 176 tons of insecticide pesticides, every mu is reduced 20 yuan of control costs.Cover whole city's tobacco planting area in 2010 and reach 100%; At present, Yuxi aphidius gifuensis control cigarette aphid technology has broad application prospects in biological control, is the core of " controlling worm with worm " nuisanceless green biological pest control system engineering.This bio-control method has been applied in Yunnan Province and has been reached 1,900,000 mu, 5,700 ten thousand of aphid kinds are provided, it is 3,500,000 mu that 2011 yearly plans are applied area, need 100,000,000 of aphid kinds, cigarette aphid over wintering mating system of the present invention can the above-mentioned sufficient amount of child care and quality cigarette aphid preferably, for a large amount of breeding in next year aphidius gifuensis is given security.
Claims (5)
1. cigarette aphid over wintering mating system comprises following sequential steps:
(a) gather the cigarette aphid:
The annual 8-10 month, select two ages of aptery greatly, no a cocoon honeybee parasitism, the infection of no germ and three ages in the cigarette aphid that will breed the warm canopy as if aphid from the big aptery cigarette aphid of field acquisition with from last one year, wherein, the ratio of the quantity of the cigarette aphid of gathering in the cigarette aphid of field acquisition and the warm canopy is 3: 7;
(b) the cigarette seedling connects aphid:
The cigarette aphid of above-mentioned collection is inoculated on the fresh cigarette seedling of the cultivation in the warm canopy or Turnip Sprouts or the green vegetables seedling breeds, every young plant connects cigarette aphid 8-12 head when connecing aphid, described fresh cigarette seedling be meant emerge back 80 days, do not inoculate the cigarette seedling of cigarette aphid, described fresh Turnip Sprouts or green vegetables seedling be meant emerge back 30 days, do not inoculate Turnip Sprouts or the green vegetables seedling of cigarette aphid;
(c) cigarette aphid detoxification:
Above-mentioned cigarette aphid breeding is after 10 days, reject in spite of illness cigarette strain and cigarette aphid, with anosis cigarette seedling or the cigarette aphid renewed vaccination on the Turnip Sprouts on fresh cigarette seedling or Turnip Sprouts or green vegetables seedling, repeat above-mentioned detoxification process 2-3 time after, filter out the cigarette seedling of stalwartness, virus-free performance and the cigarette aphid on the dish seedling;
(d) cigarette aphid purification and rejuvenation:
The above-mentioned cigarette aphid that filters out is inoculated into fresh cigarette strain bottom leaf in the warm canopy, and every strain connects 20 of cigarette aphids, and described fresh cigarette strain is meant the nine leaves cigarette strain wholeheartedly of being transplanted to behind the warm canopy 25-30 days;
Temperature in the temperature canopy is controlled at 22 ± 5 ℃, and humidity is controlled at 65%-80%, and under the natural lighting condition, breeding cigarette aphid 15-20 days can reach 2000 of individual plant cigarette aphids;
When treating that the cigarette aphid breeds 2000 of every strains, the cigarette aphid is detected, remove host plant in spite of illness, reject in spite of illness the cigarette aphid and by parasitic cigarette aphid, healthy and strong cigarette aphid is cooked the fresh cigarette strain bottom Ye Zaici that generation kind aphid receives in the warm canopy once more and purificates and rejuvenates;
(e) cigarette aphid over wintering:
After late November or early December, cigarette aphid in the above-mentioned temperature canopy is inoculated on the fresh Turnip Sprouts or green vegetables seedling in the glass greenhouse, described fresh Turnip Sprouts or green vegetables seedling are meant emerge back 30 days Turnip Sprouts or green vegetables seedling, every young plant connects cigarette aphid 8-12 head when connecing aphid, in temperature is 22 ± 5 ℃, and humidity is to continue breeding under the condition of 50%-70%;
(f) cigarette aphid expanding propagation:
In late Febuary, second moves into the numerous aphid plant in the above-mentioned glass greenhouse in the warm canopy to by the end of March, breeds in the purification and rejuvenation mode in the step (d).
2. cigarette aphid over wintering mating system according to claim 1 is characterized in that, described step (d) or (f) in the breeding method of cigarette strain of purification and rejuvenation usefulness comprise following sequential steps:
(2.1) grow seedlings the stage: the flue-cured tobacco capsuled seed of K326 or cloud and mist 203 kinds was planted in the seedling-cultivating tray in the cigarette transplantation of seedlings in preceding 70 days, adopts the floating seedlings technology to grow seedlings;
(2.2) become the seedling stage: treat the seedling strain Cheng Miaohou in the seedling-cultivating tray, it is transplanted in the basin of putting in warm canopy, the potted plant line-spacing has been 30cm * 30cm, water normal root water while planting, add 0.3kg in every 100kg normal root water and carry seed manure, carrying nitrogenous fertilizer in the seed manure, phosphate fertilizer, potash fertilizer is 12: 6: 24 by weight ratio, and nitrogenous fertilizer is nitrate nitrogen, phosphate fertilizer is phosphorus pentoxide, and potash fertilizer is potassium oxide;
Fertilising in 10 days after the cigarette transplantation of seedlings, converting water 100kg with 100 strains with 300g composite fertilizer and 100-200g urea waters and executes, spray with clear water again, in order to avoid generation fertilizer damage, nitrogenous fertilizer, phosphate fertilizer, potash fertilizer are 12: 6: 24 by weight ratio in the described composite fertilizer, nitrogenous fertilizer is ammoniacal nitrogen, phosphate fertilizer is phosphorus pentoxide, potash fertilizer is potassium oxide, later on every fertilising in 10 days once according to growing way, per 100 strain sealing fertilizer 400-500g, wherein urea, phosphorus ammonium, potassium sulphate are 12: 6: 24 by weight ratio, later on every fertilising in 10 days once, per 100 strain sealing fertilizer 400-500g according to growing way;
Water management: the seedling strain is watered sufficient normal root water when transplanting, and accomplishes that later on little water duty waters, and guarantees that basin soil is moistening, the excessive basin soil shallow lake pulpboard knot that causes of avoiding watering;
The regulation and control of temperature, wet, light: the strong fine day of illumination is rolled the film of Wen Peng side, makes warm canopy inner air convection; Launch the sunshade net and reduce illumination, reduce the temperature in the warm canopy; At dusk put down the film of side, make the interior nocturnal temperature of warm canopy be not less than 10 ℃; The cloudy day film of side is not rolled, and packs up the sunshade net simultaneously, and making has suitable illumination in the warm canopy, and temperature is not higher than 25 ℃, and humidity remains on 50%-70%;
The extermination of disease and insect pest: the anthracnose their early stage sprays and it can be killed with 500 times of liquid of 25% carbendazol wettable powder, and the powdery mildew their early stage sprays and it can be killed with 800-1000 times of liquid of 36% thiophanate-methyl suspending agent.
3. cigarette aphid over wintering mating system according to claim 2 is characterized in that:
Described temperature canopy is by conjoined green house frame (1) and cover peripheral canopy film (2) formation of greenhouse frame (1), comprise two domes, two sides and two end faces, long 50 meters, wide 12 meters of greenhouse frame (1), 3 meters of shoulder heights, 4.6 meters of sagitta, 3 meters of column (9) spacings of greenhouse frame (1), 6 meters of spans, 0.75 meter of spring base, vault is provided with three vertical poles (10); 4 cover film-coiling devices (8) are arranged on the greenhouse frame (1), wherein two covers are installed in greenhouse frame (1) both sides, other two covers are installed in the outer top of greenhouse frame (1), greenhouse frame (1) inner top is provided with 60 order sunshade nets (5), sunshade net (5) below is useful on numerous aphid chamber (6) of breeding cigarette aphid, numerous aphid chamber (6) is by framework (3) and cover peripheral fly net (4) formation of framework (3), there are two rows numerous aphid chamber (6), arrange along the interior two sides of greenhouse frame (1) respectively, the numerous aphid of every row chamber (6) has 17, wherein row numerous aphid chamber (6) is long 6 meters, wide 3 meters, high 1.8 meters, another arranges long 4.5 meters of numerous aphid chamber (6), wide 3 meters, high 1.8 meters, stay 1.5 meters wide aisle (7) between two row numerous aphid chambers (6).
4. cigarette aphid over wintering mating system according to claim 3 is characterized in that:
The cigarette basin of planting that the strain of cultivation cigarette is used in the described temperature canopy is a flowerpot, and its diameter is 25.0-30.0cm, and diameter is 15.0-17.0cm at the bottom of the basin, and the basin hem width is that the high flowerpot for 26.0cm of 1.5-2.0cm, basin is advisable as planting the cigarette basin;
The cultivating soil of planting in the cigarette basin is the gutless red soil 1000kg that crosses cigarette and vegetables, add well-rotted farmyard manure 300kg, fused calcium magnesium phosphate 20kg and fully mix thoroughly, heap is sealed the above crude opium of planting of fermentation 30d, adds carbendazim 100g, chlorophos 100g during the dress basin, fully mix thoroughly, prevent soil worm in spite of illness.
5. cigarette aphid over wintering mating system according to claim 4 is characterized in that:
When described cigarette aphid inoculates, choose the aphid inoculation with soft little writing brush;
In warm canopy or glass greenhouse, carry out in the cigarette aphid over wintering reproductive process, to prevent and kill off cigarette aphid natural enemy, the natural enemies such as prevention syrphus fly, ladybug, cigarette fleahopper and cocoon honeybee of closing the doors and windows are gone into canopy, and syrphus fly, ladybug, cigarette fleahopper manually caught, to being shifted out outside warm canopy or the glass greenhouse by the aphid kind of cocoon honeybee parasitism;
The high 3.6m of glass greenhouse in the described step (e), wide 5.3m, long 14.2m;
Temperature in the described step (e) adds thermal control by the quartzy warmer of the family expenses of a 1000W, and humidity is controlled with humidifier.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100247462A CN102172231B (en) | 2011-01-19 | 2011-01-19 | Method for overwintering species conservation propagation of myzus persicae |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2011100247462A CN102172231B (en) | 2011-01-19 | 2011-01-19 | Method for overwintering species conservation propagation of myzus persicae |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102172231A true CN102172231A (en) | 2011-09-07 |
| CN102172231B CN102172231B (en) | 2012-05-23 |
Family
ID=44515626
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2011100247462A Active CN102172231B (en) | 2011-01-19 | 2011-01-19 | Method for overwintering species conservation propagation of myzus persicae |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102172231B (en) |
Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102812933A (en) * | 2012-09-11 | 2012-12-12 | 云南省烟草公司玉溪市公司 | Overwintering preserving breeding method for aphidius gifuensis |
| CN103004705A (en) * | 2013-01-06 | 2013-04-03 | 云南省烟草农业科学研究院 | Method for large-scale breeding non-toxic myzus persicae |
| CN103109781A (en) * | 2013-03-14 | 2013-05-22 | 吉训聪 | Artificial breeding method of Taiwan abdominal cocoon bee |
| CN103766289A (en) * | 2014-01-22 | 2014-05-07 | 云南省烟草公司玉溪市公司 | Method for improving breeding efficiency of encarsia formosa |
| CN103999665A (en) * | 2014-05-09 | 2014-08-27 | 云南省烟草公司保山市公司 | Method for breeding aphidius gifuensis with courled mallow |
| CN104222008A (en) * | 2013-06-21 | 2014-12-24 | 王伯明 | Method for artificial mass production of hoverflies |
| CN104396881A (en) * | 2014-09-19 | 2015-03-11 | 中山大学 | Propylaea japonica artificial breeding, breed conservation and propagation production method |
| CN104585135A (en) * | 2014-12-21 | 2015-05-06 | 辽宁省烟草公司朝阳市公司 | Overwintering breeding method for aphidiusgifuensis in northern area |
| CN105165733A (en) * | 2015-09-25 | 2015-12-23 | 黔南州烟草公司瓮安县分公司 | Overwintering breeding method for aphidius gifuensis population |
| CN105475233A (en) * | 2015-11-24 | 2016-04-13 | 四川省烟草公司宜宾市公司 | Myzus periscae overwintering breed conservation breeding method |
| CN105519496A (en) * | 2016-02-16 | 2016-04-27 | 弥渡格瑞生物科技有限责任公司 | Natural diffusion myzus persicae vaccination method |
| CN105994171A (en) * | 2016-06-02 | 2016-10-12 | 河南省农业科学院烟草研究所 | Method for conducting aphid conservation in winter by means of winter cabbages |
| CN106234312A (en) * | 2016-08-05 | 2016-12-21 | 云南省烟草公司昆明市公司 | A kind of save space, low cost, a confession kind method for the aphidius gifuensis of economical and efficient |
| CN106305629A (en) * | 2016-08-23 | 2017-01-11 | 高啟荣 | Aphidius avenae domestication breeding and expanding propagation method |
| CN109744116A (en) * | 2019-03-25 | 2019-05-14 | 江苏徐淮地区徐州农业科学研究所(江苏徐州甘薯研究中心) | A kind of overwintering method of keeping a full stand of seedings of sweet potato seedling |
| CN112568192A (en) * | 2020-12-31 | 2021-03-30 | 昭通学院 | Research device for field occurrence of migratory insect pests into pests and research method for overwintering population of spodoptera frugiperda |
| CN114271240A (en) * | 2022-01-14 | 2022-04-05 | 北京市农林科学院 | Breeding method of adelphocoris suturalis |
| CN114788481A (en) * | 2021-01-26 | 2022-07-26 | 北京市农林科学院 | Repelling plant management method for preventing and controlling green house green peach aphids |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101455190A (en) * | 2008-12-30 | 2009-06-17 | 云南省烟草公司玉溪市公司 | Aphidius gifuensis Ashmaed scale propagation technique |
| CN101548658A (en) * | 2009-05-25 | 2009-10-07 | 云南省烟草公司大理州公司 | Method of breeding aphidius gifuensis in high-density |
| CN201336869Y (en) * | 2008-12-30 | 2009-11-04 | 云南省烟草公司玉溪市公司 | Aphidius gifuensis breeding big plastic shed |
| CN101664017A (en) * | 2009-09-30 | 2010-03-10 | 云南省烟草农业科学研究院 | Method for propagating sulzer braconid flies |
-
2011
- 2011-01-19 CN CN2011100247462A patent/CN102172231B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101455190A (en) * | 2008-12-30 | 2009-06-17 | 云南省烟草公司玉溪市公司 | Aphidius gifuensis Ashmaed scale propagation technique |
| CN201336869Y (en) * | 2008-12-30 | 2009-11-04 | 云南省烟草公司玉溪市公司 | Aphidius gifuensis breeding big plastic shed |
| CN101548658A (en) * | 2009-05-25 | 2009-10-07 | 云南省烟草公司大理州公司 | Method of breeding aphidius gifuensis in high-density |
| CN101664017A (en) * | 2009-09-30 | 2010-03-10 | 云南省烟草农业科学研究院 | Method for propagating sulzer braconid flies |
Cited By (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102812933B (en) * | 2012-09-11 | 2014-02-05 | 云南省烟草公司玉溪市公司 | Overwintering preserving breeding method for aphidius gifuensis |
| CN102812933A (en) * | 2012-09-11 | 2012-12-12 | 云南省烟草公司玉溪市公司 | Overwintering preserving breeding method for aphidius gifuensis |
| CN103004705A (en) * | 2013-01-06 | 2013-04-03 | 云南省烟草农业科学研究院 | Method for large-scale breeding non-toxic myzus persicae |
| CN103109781A (en) * | 2013-03-14 | 2013-05-22 | 吉训聪 | Artificial breeding method of Taiwan abdominal cocoon bee |
| CN103109781B (en) * | 2013-03-14 | 2015-06-17 | 吉训聪 | Artificial breeding method of Taiwan abdominal cocoon bee |
| CN104222008B (en) * | 2013-06-21 | 2016-06-01 | 首伯农(北京)生物技术有限公司 | A kind of artificial a large amount of method producing wasp fly |
| CN104222008A (en) * | 2013-06-21 | 2014-12-24 | 王伯明 | Method for artificial mass production of hoverflies |
| CN103766289A (en) * | 2014-01-22 | 2014-05-07 | 云南省烟草公司玉溪市公司 | Method for improving breeding efficiency of encarsia formosa |
| CN103999665A (en) * | 2014-05-09 | 2014-08-27 | 云南省烟草公司保山市公司 | Method for breeding aphidius gifuensis with courled mallow |
| CN103999665B (en) * | 2014-05-09 | 2016-08-24 | 云南省烟草公司保山市公司 | A kind of method with certain herbaceous plants with big flowers dish cultivation aphidius gifuensis |
| CN104396881A (en) * | 2014-09-19 | 2015-03-11 | 中山大学 | Propylaea japonica artificial breeding, breed conservation and propagation production method |
| CN104585135A (en) * | 2014-12-21 | 2015-05-06 | 辽宁省烟草公司朝阳市公司 | Overwintering breeding method for aphidiusgifuensis in northern area |
| CN105165733A (en) * | 2015-09-25 | 2015-12-23 | 黔南州烟草公司瓮安县分公司 | Overwintering breeding method for aphidius gifuensis population |
| CN105475233A (en) * | 2015-11-24 | 2016-04-13 | 四川省烟草公司宜宾市公司 | Myzus periscae overwintering breed conservation breeding method |
| CN105475233B (en) * | 2015-11-24 | 2018-07-03 | 四川省烟草公司宜宾市公司 | A kind of cigarette aphid over wintering mating system |
| CN105519496B (en) * | 2016-02-16 | 2017-11-21 | 弥渡格瑞生物科技有限责任公司 | A kind of natural diffuseness of cigarette aphid connects aphid method |
| CN105519496A (en) * | 2016-02-16 | 2016-04-27 | 弥渡格瑞生物科技有限责任公司 | Natural diffusion myzus persicae vaccination method |
| CN105994171A (en) * | 2016-06-02 | 2016-10-12 | 河南省农业科学院烟草研究所 | Method for conducting aphid conservation in winter by means of winter cabbages |
| CN105994171B (en) * | 2016-06-02 | 2019-06-04 | 河南省农业科学院烟草研究所 | A kind of method carrying out aphid winter conservation using Chinese cabbage is stored away in winter |
| CN106234312A (en) * | 2016-08-05 | 2016-12-21 | 云南省烟草公司昆明市公司 | A kind of save space, low cost, a confession kind method for the aphidius gifuensis of economical and efficient |
| CN106234312B (en) * | 2016-08-05 | 2019-02-26 | 云南省烟草公司昆明市公司 | A kind of save space, low cost, the aphidius gifuensis of economical and efficient for kind of a method |
| CN106305629A (en) * | 2016-08-23 | 2017-01-11 | 高啟荣 | Aphidius avenae domestication breeding and expanding propagation method |
| CN106305629B (en) * | 2016-08-23 | 2019-05-21 | 高啟荣 | Aphid parasite, which is tamed and dociled, educates expanding propagation method |
| CN109744116A (en) * | 2019-03-25 | 2019-05-14 | 江苏徐淮地区徐州农业科学研究所(江苏徐州甘薯研究中心) | A kind of overwintering method of keeping a full stand of seedings of sweet potato seedling |
| CN112568192A (en) * | 2020-12-31 | 2021-03-30 | 昭通学院 | Research device for field occurrence of migratory insect pests into pests and research method for overwintering population of spodoptera frugiperda |
| CN114788481A (en) * | 2021-01-26 | 2022-07-26 | 北京市农林科学院 | Repelling plant management method for preventing and controlling green house green peach aphids |
| CN114788481B (en) * | 2021-01-26 | 2023-07-28 | 北京市农林科学院 | Plant-repelling management method for preventing and controlling green house green peach aphids |
| CN114271240A (en) * | 2022-01-14 | 2022-04-05 | 北京市农林科学院 | Breeding method of adelphocoris suturalis |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102172231B (en) | 2012-05-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102172231B (en) | Method for overwintering species conservation propagation of myzus persicae | |
| CN102812933B (en) | Overwintering preserving breeding method for aphidius gifuensis | |
| CN103270918B (en) | Rice and shrimp joint culturing ecological breeding system and method | |
| CN102301982B (en) | Method for propagating Aphidius gifuensis by pea aphids | |
| CN103385220B (en) | Scale breeding method for encarsia formosa | |
| CN103168751B (en) | Expanding propagation method for aphidius gifuensis ashmeads | |
| CN106258755B (en) | A kind of implantation methods of the yellow jacket tree forest interplanting bletilla striata | |
| CN105475233B (en) | A kind of cigarette aphid over wintering mating system | |
| CN105104213B (en) | Rapid propagation and seedling culture method using rhizomes of paris polyphylla | |
| CN103960102A (en) | Method for cultivating high-yield mid-autumn crisp jujubes | |
| CN102334468A (en) | High-efficiency floating seedling Aphidius gifuensis propagation method | |
| CN105027910A (en) | Ascendens mucronatum cuttage technology | |
| CN104798682B (en) | A kind of balsam pear breeding cultivation method | |
| CN103548635A (en) | Pollution-free culturing method for increasing raspberry yield | |
| CN106689066A (en) | Method for quickly breeding aphidius gifuensis | |
| CN103202165A (en) | Method for cutting and raising seedlings of cliff tea | |
| CN105123253B (en) | A kind of method for culturing seedlings of paris polyphylla | |
| CN105165313B (en) | Aphidius gifuensis short cycle mating system | |
| CN101595857B (en) | Artificial large-scale feeding method of ophraella communa lesage | |
| CN104969766A (en) | Method for planting strawberry | |
| CN102893786A (en) | Paddy-upland rotation culture method for strawberries and water chestnuts | |
| CN106305276A (en) | Cyclocarya paliurus root cutting propagation method | |
| CN111466263A (en) | Grafting seedling method for pepper cultivation in sunlight greenhouse | |
| CN102792831B (en) | High-efficiency rapid propagation technique for Chinese yew | |
| CN105340836A (en) | Method for improving factory propagation efficiency of encarsia formosa |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |