CN105475233A - Myzus periscae overwintering breed conservation breeding method - Google Patents
Myzus periscae overwintering breed conservation breeding method Download PDFInfo
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- CN105475233A CN105475233A CN201510823700.5A CN201510823700A CN105475233A CN 105475233 A CN105475233 A CN 105475233A CN 201510823700 A CN201510823700 A CN 201510823700A CN 105475233 A CN105475233 A CN 105475233A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Abstract
The invention discloses a Myzus periscae overwintering breed conservation breeding method including the following steps of: (1) performing collection, detoxification, purification and renovation on Myzus periscae, and obtaining robust and disease-free Myzus periscae; (2) inoculating the Myzus periscae on fresh radish sprouts or green vegetable sprouts in a glass greenhouse for breeding in November every year; (3) inoculating the Myzus periscae which is on the fresh radish sprouts or green vegetable sprouts onto tobacco flowers in the glass greenhouse in February of the second year, and breeding the Myzus periscae on the tobacco flowers for 10-15 days; and (4) inoculating the Myzus periscae which is on the tobacco flowers onto bottom leaves of tobacco plants in a plastic shed and performing expanding propagation on the Myzus periscae. According to the invention, the tobacco flowers are used as transitional hosts. The tobacco flowers are the parts of the tobacco plants with the lowest nicotine content. The tobacco flowers contain honeydew, and attract the Myzus periscae to eat, and thus the activity and fertility of the Myzus periscae can be ensured. After the method of the invention is used, the survival rate of the Myzus periscae inoculated onto the tobacco leaves is raised to 70% from 10% in the prior art. The egg laying amount, after the Myzus periscae is inoculated with aphidius gifuensis for 7 days, is 60-70 times of the egg laying amount in the prior art.
Description
Technical field
The invention belongs to the technical field of aphidius gifuensis control, be specifically related to a kind of cigarette aphid over wintering mating system.
Background technology
At present, use aphidius gifuensis control cigarette aphid to achieve good effect, reach the object of " controlling worm with worm ".Aphidius gifuensis is the natural enemy of cigarette aphid, and cigarette aphid is the host of aphidius gifuensis.Ovum, when mate and oviposit, can produce in cigarette aphid body by aphidius gifuensis, and aphidius gifuensis develops into adult by the internal organ taken food in cigarette aphid body, thus is killed by cigarette aphid, reaches the object eliminated destructive insects.
Prevent and treat in applying of cigarette aphid at aphidius gifuensis, need to breed a large amount of aphidius gifuensis, large-scaled propugation will be carried out to aphidius gifuensis, but before large-scaled propugation is carried out to aphidius gifuensis, a large amount of hosts-cigarette aphid need be obtained.Known one section of patent application (number of patent application: 201110024746.2) propose a kind of cigarette aphid over wintering mating system, its method comprises the following steps: (1) summer and autumn cigarette aphid is gathered, detoxification, purification and rejuvenation, and to breed in the strain of warm canopy cigarette; (2) winter pre-incubation Turnip Sprouts, green vegetables seedling in glass greenhouse; (3) late November is to early December, and be inoculated into by cigarette aphid in warm canopy on Turnip Sprouts in glass greenhouse or green vegetables seedling and continue breeding, control warm indoor temperature at 22 ± 5 DEG C, humidity is 50-70%; (4) Second Year March, cigarette aphid in greenhouse being inoculated into cigarette strain inferior leads in warm canopy, to carry out expansion numerous.But there is following shortcoming in the method: the core of (1) the method needs to control the temperature 22 ± 5 DEG C in greenhouse, humidity 50-70%, this condition in the winter time minimum air temperature is that the area of less than 0 DEG C or 0 DEG C can only lean on warming and humidifying equipment to realize, and remote mountain areas cannot ensure supply of electric power, be difficult to ensure temp. and humidity; (2) Turnip Sprouts, green vegetables seedling be not all containing nicotine, utilizes the cigarette aphid that its conservation is cultivated, be inoculated into after on tobacco leaf, because composition of food changes, very easily occurs that cigarette aphid does not take food, do not breed, active low, even dead phenomenon, causes over wintering failed.
Summary of the invention
For solving the deficiency that in prior art, cigarette aphid over wintering mating system exists, the invention provides a kind of cigarette aphid over wintering mating system.
For achieving the above object, the invention provides a kind of cigarette aphid over wintering mating system, the method comprises the following steps:
(1) cigarette aphid is gathered, detoxification, purification and rejuvenation, obtain healthy and strong anosis cigarette aphid;
(2) annual November, above-mentioned cigarette aphid is inoculated on fresh Turnip Sprouts in glass greenhouse or green vegetables seedling and breeds;
(3) Second Year February, the cigarette aphid on above-mentioned Turnip Sprouts or green vegetables seedling is inoculated on the fireworks in glass greenhouse, makes it on fireworks, breed 10-15 days;
(4) the cigarette aphid on above-mentioned fireworks being inoculated into cigarette strain inferior leads in warm canopy, to carry out expansion numerous.
In step (1), the annual 6-8 month, if from field acquisition cigarette aphid aptery greatly and select warm canopy healthy two anosis ages and three age aphid, the number ratio of the cigarette aphid gathered in the cigarette aphid of field acquisition and greenhouse is 3 ~ 4:6 ~ 7.
In step (1), to the method for cigarette aphid detoxification be: be inoculated in by the cigarette aphid of collection in the anosis cigarette strain wholeheartedly of nine leaves and breed, every strain connects aphid 20-25 head; Breed after 10-15 days, reject cigarette strain in spite of illness and cigarette aphid, anosis cigarette aphid is inoculated on fresh Turnip Sprouts or green vegetables seedling, after repeating said process 2-3 time, filter out the cigarette aphid that stalwartness is anosis.
In step (1), to the method for cigarette aphid purification and rejuvenation be: be inoculated in the fresh tobacco plant in warm canopy by cigarette aphid anosis for stalwartness, every strain connects cigarette aphid 20-25 head, temperature 20-28 DEG C in warm canopy, humidity 60-80%, under natural lighting condition, breeding cigarette aphid 20-25 days; Afterwards cigarette aphid is detected, select healthy and strong anosis cigarette aphid and be again inoculated in the fresh tobacco plant in warm canopy, again purificate and rejuvenate.
In step (2), in glass greenhouse, plastic film is used to build little Wen Peng, in annual October potted plant Turnip Sprouts or green vegetables seedling in little Wen Peng.
In step (2), fresh Turnip Sprouts or green vegetables seedling refer to the Turnip Sprouts or green vegetables seedling of emerging latter 30 days, and every strain Turnip Sprouts or green vegetables seedling connect cigarette aphid 10-15 head, at temperature 20-28 DEG C, breed under the condition of humidity 60-80%.
In step (3), the breeding method of fireworks is: in Second Year potted plant K326 cigarette seedling in glass greenhouse in January, cultivate after 40-50 days, fertilizer of cutting off the water supply, moved on in the low temperature environment of outer 5-10 DEG C of glass greenhouse and cultivated, moved on to again in glass greenhouse after making its early blossoming.
Preferably, the cultivating soil of potted plant K326 cigarette seedling is by the red soil of not planting cigarette and vegetables, add well-rotted farmyard manure, fused calcium magnesium phosphate mixes, heap is sealed fermentation 30-50d and is obtained, and the mass ratio of red soil, well-rotted farmyard manure, fused calcium magnesium phosphate is 100:20-40:1-3.
Beneficial effect of the present invention is:
(1) because nicotine has obvious inhibitory action to cigarette aphid, the radish leaves that cigarette aphid overwintering period takes food, green vegetable leaf be not all containing nicotine, directly the cigarette aphid that survives the winter on Turnip Sprouts, green vegetables seedling is inoculated in cigarette strain, cigarette aphid has adapted to the food without nicotine, the tobacco leaf taken food containing nicotine can be resisted from instinct, thus cause that it is active, fertility obviously declines, even dead.The present invention utilizes fireworks to be inoculated into transitional host tobacco leaf from radish, green vegetables as cigarette aphid, and fireworks are parts that in cigarette strain, nicotine content is minimum, and containing honeydew, cigarette aphid can be attracted to take food, and makes the food that cigarette aphid adapts to containing nicotine gradually; Be inoculated on tobacco leaf by cigarette aphid from fireworks more afterwards, activity, the fertility of cigarette aphid all can be guaranteed.The results showed, after using method of the present invention, cigarette aphid is inoculated into the survival rate on tobacco leaf and brings up to 70% from 10% of prior art, and the egg laying amount of inoculation cocoon honeybee after 7 days is 60-70 times of prior art.
(2) in glass greenhouse, utilize plastic film to build little temperature canopy can set up second layer thermal environment, the bulk temperature comparatively outer high 5-8 DEG C of canopy in little temperature canopy; Go wrong period in supply of electric power, airtight little temperature canopy, can ensure in 20-30 hour that little temperature of shed is more than 15 DEG C, effective guarantee host plant and cigarette aphid vigor.
Embodiment
A kind of cigarette aphid over wintering mating system of the present invention, the method comprises the following steps:
1, cigarette aphid is gathered, detoxification, purification and rejuvenation, obtain healthy and strong anosis cigarette aphid.
1.1 gather cigarette aphid
The annual 6-8 month, if from field acquisition cigarette aphid aptery greatly and select from the cigarette aphid that Wen Peng breeds healthy two anosis ages and three age aphid, the number ratio of the cigarette aphid of field acquisition and the cigarette aphid of warm canopy collection is 3 ~ 4:6 ~ 7; If two age aphid be the long 0.8-1mm of body, the wide 0.4-0.5mm of body, the larva in pistac, if three age aphid be the long 1.2-1.3mm of body, the wide 0.6-0.65mm of body, in red green or pink larva.
1.2 cigarette seedlings connect aphid
Nine leaves cigarette aphid of above-mentioned collection being inoculated in rearing technology 25-30 days anosis cigarette strain is wholeheartedly bred, connects aphid and carry out according to the standard of every strain 20-25 head; During the inoculation of cigarette aphid, carry out choosing aphid inoculation with the little writing brush of softness.
1.3 cigarette aphid detoxifications
Above-mentioned cigarette aphid breeding is after 10-15 days, reject cigarette strain in spite of illness and cigarette aphid, anosis cigarette aphid is inoculated on fresh Turnip Sprouts or green vegetables seedling, after repeating above-mentioned During Detoxification 2-3 time, filter out cigarette aphid that is healthy and strong, virus-free performance, described fresh Turnip Sprouts or green vegetables seedling refer to emerge latter 30 days, the Turnip Sprouts of not inoculated cigarette aphid or green vegetables seedling.
Wild tobacco aphid is generally with arbovirus such as cucumber mosaic virus, marmor upsilon, etch virus poison, if do not carry out detoxification treatment, cigarette strain after inoculation aphid will infect virus disease, not only affect the breeding amount of cigarette aphid and aphidius gifuensis, increase cost, also can inoculate cigarette aphid, when expanding numerous, continue transmitted virus, by said method, can reject cigarette aphid and cigarette strain in spite of illness, the cigarette aphid of healthy and strong, virus-free performance is used for inoculating next time.
1.4 cigarette aphid purification and rejuvenation
The above-mentioned cigarette aphid filtered out is inoculated into the fresh tobacco plant middle and lower part leaf in warm canopy, every strain connects cigarette aphid 20-25 head, and described fresh tobacco plant refers to the nine leaves wholeheartedly cigarette strain of the group's of being in phase.
Temperature in temperature canopy controls at 20-28 DEG C, and humid control is at 60-80%, and under natural lighting condition, breeding cigarette aphid 20-25 days, can reach individual plant cigarette aphid 2000.
When cigarette aphid breeds every strain 2000, detect cigarette aphid, remove band virus vector plant, reject cigarette aphid and by parasitic cigarette aphid in spite of illness, healthy and strong cigarette aphid is cooked generation kind aphid and again receives fresh tobacco plant inferior leads in warm canopy, again purificates and rejuvenates.
2, above-mentioned cigarette aphid is inoculated on fresh Turnip Sprouts in glass greenhouse or green vegetables seedling and breeds.
2.1 hibernacles prepare
In glass greenhouse, utilize plastic film to build little Wen Peng, bottom little temperature canopy, pad waste and old drift dish; And in October potted plant Turnip Sprouts or green vegetables seedling in little Wen Peng.
Described glass greenhouse is made up of greenhouse frame and the glass covering greenhouse frame periphery, comprise a dome, two sides and two end faces, long 40 meters of greenhouse frame, wide 8 meters, shoulder height 3 meters, sagitta 4.8 meters, the column of greenhouse frame is in central authorities, vault is provided with a vertical pole, the numerous aphid room for breeding cigarette aphid is had in greenhouse, numerous aphid room is made up of framework and the PE film and fly net covering frame peripheral, there are two rows numerous aphid room, respectively along two sides arrangement in greenhouse frame, often arranging numerous aphid room has between 12, often arrange numerous aphid room and be long 3 meters, wide 3 meters, high 2 meters, the aisle of 2 meters wide is stayed between the two numerous aphid rooms of row, glass greenhouse configuration natural ventilation system, external sunshade system, internal sunshade system, top are windowed, air-conditioning heating and cooling system, Inner eycle blower fan, heavy-current control system, computer control system, supply and drain water system etc.
2.2 cigarette aphid over winterings
November, cigarette aphid in above-mentioned temperature canopy is inoculated on fresh Turnip Sprouts in glass greenhouse or green vegetables seedling, described fresh Turnip Sprouts or green vegetables seedling refer to the Turnip Sprouts or green vegetables seedling of emerging latter 30 days, when connecing aphid, every young plant connects cigarette aphid 10-15 head, at temperature 20-28 DEG C, continue breeding under the condition of humidity 60-80%, temperature is controlled by air-conditioning, and humidity is controlled by humidifier.
Cigarette strain is best suited for the host plant of cigarette aphid breeding, but winter planting is not only time-consuming with the strain of cultivation cigarette, and poor growth, also the needs of the detoxification of cigarette aphid and purification and rejuvenation cannot be met, so selection Turnip Sprouts, green vegetables seedling are the host plant of cigarette aphid conservation in winter, the demand of cigarette aphid conservation in winter can be adapted to.
3, the cigarette aphid on above-mentioned Turnip Sprouts or green vegetables seedling is inoculated on the fireworks in glass greenhouse, makes it on fireworks, breed 10-15 days.
3.1 transitional host plants are cultivated:
Second Year January, in glass greenhouse, potted plant a collection of K326 cigarette seedling, cultivated after 40-50 days, fertilizer of cutting off the water supply, and was moved on in the low temperature environment of outer 5-10 DEG C of glass greenhouse and cultivated, move on in greenhouse after making its early blossoming again.
The flowerpot diameter of described potted plant cigarette seedling is 50cm, and basin diameter is 35-40cm, and basin hem width is 1.5-2.0cm, basin height is 30-35cm.
During described cigarette aphid inoculation, carry out choosing aphid inoculation with the little writing brush of softness.
The cultivating soil of potted plant K326 cigarette seedling, by the red soil of not planting cigarette and vegetables, adds well-rotted farmyard manure, fused calcium magnesium phosphate mixing mixed thoroughly, and it is obtained that heap seals fermentation 30-50d, adds well-rotted farmyard manure 200-400g, fused calcium magnesium phosphate 10-30g in every kg red soil.
3.2 cigarette aphid adaptability transition:
Cigarette aphid on Turnip Sprouts or green vegetables seedling is inoculated on the transition cigarette strain fireworks of above-mentioned cultivation, makes it on fireworks, breed 10-15 days;
4, cigarette aphid expanding propagation:
Second Year March, the numerous aphid plant in glass greenhouse is moved on in warm canopy, breeds in the purification and rejuvenation mode in step 1.4.
Wherein, the cigarette strain breeding method of purification and rejuvenation comprises the following steps:
The nursery stage: the flue-cured tobacco capsuled seed of K326 or cloud and mist 203 kind is planted in seedling-cultivating tray in first 70 days in cigarette transplantation of seedlings, adopts moistening seedling growing process nursery;
In the seedling stage: after the cigarette strain seedling in seedling-cultivating tray, transplanted in the flowerpot that sets in warm canopy, potted plant distance is 50cm × 50cm; Base fertilizer has been executed according to the standard of every strain 50g composite fertilizer special and 25g superphosphate before transplanting; After transplanting, sufficient 250g normal root water is watered in every strain, adds 0.3kg Special compound fertilizer for tobacco in every 100kg normal root water.Described specific complex fertilizer formula is N:P
2o
5: K
2o=10%:15%:25%, every bag of 50kg.
Cigarette transplantation of seedlings, after 10 days, is carried out first time and is topdressed, and is watered 100kg with 500g composite fertilizer and special the topdressing of 100g, waters execute after mixing according to the standard of every strain 1kg, waters when executing and notes avoiding the tender position of cigarette strain children; Described specific complex fertilizer formula is N:P
2o
5: K
2o=10%:15%:25%, every bag of 50kg; The special formula that topdresses is for every bag of 15kg, containing magnesium sulfate 2.5kg, zinc sulphate 1kg, boric acid 0.5kg, potassium nitrate 6.75kg, potassium chloride 0.75kg, ammonium nitrate phosphorus 3.5kg; Potassium sulfate K
2o content is 50%; Potassium nitrate K
2o content is 44.5%, and nitrogen content is 13.5%; Normal superphosphate P
2o
5content is 12%.
Water management: according to weather condition after transplanting, waters a water for 2-3 days, is as the criterion to irrigate.
Temperature, wet, light regulation and control: meet day with high temperature according to weather, launch double-layered sunshade net and reduce illumination and temperature of shed, roll Wen Peng side film, keep ventilating, put down side film in the evening again; Cloudy day packs up sunshade net, and side film is not rolled; Keep temperature 25-28 DEG C, humidity 50-70%.
The extermination of disease and insect pest: anthracnose their early stage 58% first frost mancozeb wettable powder, 1000 times of liquid foliar sprays; Virus disease can take 8% Cystosine nucleocide type antibiotics (Ningnanmycin) aqua, 1600 times of liquid or 24% mixed fat copper sulphate aqueous emulsion 1000 times of liquid foliar sprays; Powdery mildew their early stage 36% thiophanate-methyl suspending agent 800-1000 times of liquid foliar spray; Bacterial wilt uses 72% agricultural streptomycin soluble powder 3500-5000 times of liquid to carry out the control of filling root after transplanting; The also unified use 48% downy mildew network ammonia copper aqua 1500 times of liquid irrigating roots controls after seedling of balck shank cigarette seedling.
Be the method described in the patent of 201110024746.2 by cigarette aphid over wintering mating system of the present invention and number of patent application of the prior art, breeding the cigarette aphid obtained is inoculated on the tobacco leaf in warm canopy, according to the standard inoculation of every strain 20, other treatment conditions are consistent; Add up survival volume after 1 day, calculate survival rate, statistical disposition cigarette strain 100 strain.Be inoculated on the tobacco leaf in warm canopy by breeding the cigarette aphid obtained, according to the standard inoculation of every strain 20, then be inoculated in this host's cigarette strain by individual large aseptic wild cocoon honeybee, according to the standard inoculation of every strain 20, other treatment conditions are consistent; Within 7 days, add up the egg laying amount of aphidius gifuensis afterwards, statistical disposition cigarette strain 100 strain.Statistics is as shown in table 1.As shown in Table 1, after process of the present invention, the survival rate of cigarette aphid brings up to 70% from 10% of prior art, and the egg laying amount of inoculation cocoon honeybee after 7 days is 66.7 times of prior art.
Impact on cigarette aphid after table 1 method of the present invention and existing method process
| Survival volume (head)/strain | Survival rate/strain | Egg laying amount (grain)/strain | |
| Method of the present invention | 14 | 70％ | 1000 |
| Existing method | 2 | 10％ | 15 |
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for a person skilled in the art, the present invention can have various modifications and variations.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (8)
1. a cigarette aphid over wintering mating system, it is characterized in that, the method comprises the following steps:
(1) cigarette aphid is gathered, detoxification, purification and rejuvenation, obtain healthy and strong anosis cigarette aphid;
(2) annual November, above-mentioned cigarette aphid is inoculated on fresh Turnip Sprouts in glass greenhouse or green vegetables seedling and breeds;
(3) Second Year February, the cigarette aphid on above-mentioned Turnip Sprouts or green vegetables seedling is inoculated on the fireworks in glass greenhouse, makes it on fireworks, breed 10-15 days;
(4) the cigarette aphid on above-mentioned fireworks being inoculated into cigarette strain inferior leads in warm canopy, to carry out expansion numerous.
2. cigarette aphid over wintering mating system according to claim 1, it is characterized in that, in step (1), the annual 6-8 month, if from field acquisition cigarette aphid aptery greatly and select warm canopy healthy two anosis ages and three age aphid, the number ratio of the cigarette aphid gathered in the cigarette aphid of field acquisition and greenhouse is 3 ~ 4:6 ~ 7.
3. cigarette aphid over wintering mating system according to claim 1, is characterized in that, in step (1), to the method for cigarette aphid detoxification is: be inoculated in by the cigarette aphid of collection in the anosis cigarette strain wholeheartedly of nine leaves and breed, every strain connects aphid 20-25 head; Breed after 10-15 days, reject cigarette strain in spite of illness and cigarette aphid, anosis cigarette aphid is inoculated on fresh Turnip Sprouts or green vegetables seedling, after repeating said process 2-3 time, filter out the cigarette aphid that stalwartness is anosis.
4. cigarette aphid over wintering mating system according to claim 1, it is characterized in that, in step (1), to the method for cigarette aphid purification and rejuvenation be: cigarette aphid anosis for stalwartness is inoculated in the fresh tobacco plant in warm canopy, every strain connects cigarette aphid 20-25 head, the temperature 20-28 DEG C in warm canopy, humidity 60-80%, under natural lighting condition, breeding cigarette aphid 20-25 days; Afterwards cigarette aphid is detected, select healthy and strong anosis cigarette aphid and be again inoculated in the fresh tobacco plant in warm canopy, again purificate and rejuvenate.
5. cigarette aphid over wintering mating system according to claim 1, is characterized in that, in step (2), in glass greenhouse, uses plastic film to build little Wen Peng, in annual October potted plant Turnip Sprouts or green vegetables seedling in little Wen Peng.
6. cigarette aphid over wintering mating system according to claim 1, it is characterized in that, in step (2), fresh Turnip Sprouts or green vegetables seedling refer to the Turnip Sprouts or green vegetables seedling of emerging latter 30 days, every strain Turnip Sprouts or green vegetables seedling connect cigarette aphid 10-15 head, at temperature 20-28 DEG C, breed under the condition of humidity 60-80%.
7. cigarette aphid over wintering mating system according to claim 1, it is characterized in that, in step (3), the breeding method of fireworks is: in Second Year potted plant K326 cigarette seedling in glass greenhouse in January, cultivate after 40-50 days, to cut off the water supply fertilizer, moved on in the low temperature environment of outer 5-10 DEG C of glass greenhouse and cultivate, move on to again in glass greenhouse after making its early blossoming.
8. cigarette aphid over wintering mating system according to claim 7, it is characterized in that, the cultivating soil of potted plant K326 cigarette seedling is by the red soil of not planting cigarette and vegetables, add well-rotted farmyard manure, fused calcium magnesium phosphate mixes, heap is sealed fermentation 30-50d and is obtained, and the mass ratio of red soil, well-rotted farmyard manure, fused calcium magnesium phosphate is 100:20-40:1-3.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105994172A (en) * | 2016-06-02 | 2016-10-12 | 河南省农业科学院烟草研究所 | Method for conducting aphid conservation in winter by means of winter turnips |
| CN105994171A (en) * | 2016-06-02 | 2016-10-12 | 河南省农业科学院烟草研究所 | Method for conducting aphid conservation in winter by means of winter cabbages |
| CN106472430A (en) * | 2016-10-21 | 2017-03-08 | 延边朝鲜族自治州农业科学院（延边特产研究所） | A kind of cold district aphidius gifuensis winter protecting method |
| CN106614392A (en) * | 2016-09-29 | 2017-05-10 | 中国烟草总公司陕西省公司 | Localization method for breeding and releasing of aphidius gifuensis ashmead in Shaanxi area |
| CN106689068A (en) * | 2017-01-11 | 2017-05-24 | 湖北省烟草公司恩施州公司 | Method for conserving breeds of Aphidius gifuensis in winter |
| CN109122598A (en) * | 2018-10-31 | 2019-01-04 | 云南省烟草公司文山州公司 | A kind of method of Turnip Sprouts breeding cigarette aphid |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102172231A (en) * | 2011-01-19 | 2011-09-07 | 云南省烟草公司玉溪市公司 | Method for overwintering species conservation propagation of myzus persicae |
| JP2013111008A (en) * | 2011-11-29 | 2013-06-10 | Hanawa No Kurahashi:Kk | Device for rearing insect |
| CN103168751A (en) * | 2013-04-07 | 2013-06-26 | 中国农业科学院植物保护研究所 | Expanding propagation method for aphidius gifuensis ashmeads |
| CN103392674A (en) * | 2013-07-02 | 2013-11-20 | 遵义市烟草公司绥阳县分公司 | Method for breeding aphidiidae through flue-cured tobacco seedling cultivation greenhouse |
| KR20140079664A (en) * | 2012-12-18 | 2014-06-27 | 경기도 | A stick insect breeding kit for educational experience |
| CN203913152U (en) * | 2013-12-31 | 2014-11-05 | 陈文龙 | The automatic continuous releasing device of aphid cocoon honeybee |
| CN203934526U (en) * | 2014-07-15 | 2014-11-12 | 山东临沂烟草有限公司 | Aphidius gifuensis artificial propagation chamber |
| CN104585135A (en) * | 2014-12-21 | 2015-05-06 | 辽宁省烟草公司朝阳市公司 | Overwintering breeding method for aphidiusgifuensis in northern area |
-
2015
- 2015-11-24 CN CN201510823700.5A patent/CN105475233B/en not_active Expired - Fee Related
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102172231A (en) * | 2011-01-19 | 2011-09-07 | 云南省烟草公司玉溪市公司 | Method for overwintering species conservation propagation of myzus persicae |
| JP2013111008A (en) * | 2011-11-29 | 2013-06-10 | Hanawa No Kurahashi:Kk | Device for rearing insect |
| KR20140079664A (en) * | 2012-12-18 | 2014-06-27 | 경기도 | A stick insect breeding kit for educational experience |
| CN103168751A (en) * | 2013-04-07 | 2013-06-26 | 中国农业科学院植物保护研究所 | Expanding propagation method for aphidius gifuensis ashmeads |
| CN103392674A (en) * | 2013-07-02 | 2013-11-20 | 遵义市烟草公司绥阳县分公司 | Method for breeding aphidiidae through flue-cured tobacco seedling cultivation greenhouse |
| CN203913152U (en) * | 2013-12-31 | 2014-11-05 | 陈文龙 | The automatic continuous releasing device of aphid cocoon honeybee |
| CN203934526U (en) * | 2014-07-15 | 2014-11-12 | 山东临沂烟草有限公司 | Aphidius gifuensis artificial propagation chamber |
| CN104585135A (en) * | 2014-12-21 | 2015-05-06 | 辽宁省烟草公司朝阳市公司 | Overwintering breeding method for aphidiusgifuensis in northern area |
Non-Patent Citations (1)
| Title |
|---|
| 秦焕菊等: "烟草次生物质-烟碱对烟蚜影响的研究", 《中国烟草学报》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105994172A (en) * | 2016-06-02 | 2016-10-12 | 河南省农业科学院烟草研究所 | Method for conducting aphid conservation in winter by means of winter turnips |
| CN105994171A (en) * | 2016-06-02 | 2016-10-12 | 河南省农业科学院烟草研究所 | Method for conducting aphid conservation in winter by means of winter cabbages |
| CN105994172B (en) * | 2016-06-02 | 2019-03-26 | 河南省农业科学院烟草研究所 | A kind of method carrying out aphid winter conservation using radish is stored away in winter |
| CN105994171B (en) * | 2016-06-02 | 2019-06-04 | 河南省农业科学院烟草研究所 | A kind of method carrying out aphid winter conservation using Chinese cabbage is stored away in winter |
| CN106614392A (en) * | 2016-09-29 | 2017-05-10 | 中国烟草总公司陕西省公司 | Localization method for breeding and releasing of aphidius gifuensis ashmead in Shaanxi area |
| CN106472430A (en) * | 2016-10-21 | 2017-03-08 | 延边朝鲜族自治州农业科学院（延边特产研究所） | A kind of cold district aphidius gifuensis winter protecting method |
| CN106689068A (en) * | 2017-01-11 | 2017-05-24 | 湖北省烟草公司恩施州公司 | Method for conserving breeds of Aphidius gifuensis in winter |
| CN109122598A (en) * | 2018-10-31 | 2019-01-04 | 云南省烟草公司文山州公司 | A kind of method of Turnip Sprouts breeding cigarette aphid |
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