CN112450155A - Indoor culture and subculture propagation method for yellow wild rice borers - Google Patents
Indoor culture and subculture propagation method for yellow wild rice borers Download PDFInfo
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- CN112450155A CN112450155A CN202011309036.XA CN202011309036A CN112450155A CN 112450155 A CN112450155 A CN 112450155A CN 202011309036 A CN202011309036 A CN 202011309036A CN 112450155 A CN112450155 A CN 112450155A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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Abstract
The invention discloses an indoor culture and subculture propagation method for yellow wild borers, which comprises the following steps: (1) indoor yellow wild rice borer breeding: collecting larvae of yellow-tail borer in the wild, and culturing the larvae indoors, wherein the used feed is fresh aquilaria sinensis leaves subjected to moisturizing treatment; the pupa stage is provided with loose and wet clean sandy soil after sterilization; (2) indoor secondary propagation of yellow-eared rice stem borers: and carrying out male-female pairing by utilizing the successfully eclosion adult yellow-eared moths cultured indoors, and carrying out subculture propagation breeding on the larvae hatched after the mating female moths lay eggs. The method has simple equipment and process requirements, the obtained yellow-eared moth can provide a research insect source for a laboratory, and the reference can be provided for the biological control of the aquilaria sinensis planting industry by researching the prevention and control method of the yellow-eared moth.
Description
Technical Field
The invention relates to an indoor culture and subculture propagation method for yellow wild rice borers, and belongs to the field of plant protection.
Background
Yellow wild rice borer (yellow wild rice borer) (1)Heortia vitessoidesMoore) genus Lepidoptera (Lepidotera) family of Bombycidae (Pyralidae)The insect is a foliar-feeding pest which is most seriously harmful in aquilaria sinensis planting areas in China in recent years. It is now found to be only harmful to aquilaria sinensis. The larva colony bites the aquilaria sinensis leaves, and has the characteristics of explosive property and quick feeding property, and the number of the single plant insect population ranges from hundreds to thousands. Because the density of insect population is high, the growth speed is high, the leaves of the aquilaria sinensis can be eaten within a few days, even the trunk and branch cortex can be gnawed, once the insects occur, the insects can quickly grow disastrous, the aquilaria sinensis forest is bald and has no leaves, and even the whole aquilaria sinensis forest dies.
In recent years, along with the increasing enlargement of the area of artificially and massively planting aquilaria sinensis in Hainan, Guangdong, Yunnan and the like, the harmfulness of yellow-striped rice borers is more and more serious, and according to statistics in Guangdong Huazhou regions, when the yellow-striped rice borers are serious, the damaged plant rate of the linaloe trees can reach more than 90 percent, so that the forest landscape and the healthy growth of the aquilaria sinensis are influenced, serious seedling withering and death are caused, and the economic loss is extremely high. In addition, the technology of promoting the aquilaria sinensis to be in a whole body and generate the fragrance through artificial infusion which is vigorously popularized in Guangdong, Hainan and the like utilizes the transpiration effect of the leaves of the aquilaria sinensis to convey liquid medicine to the whole tree and then to be in a whole body and generate the fragrance, but the application and popularization of the technology of promoting the aquilaria sinensis to be in a whole body and generate the fragrance are severely limited due to the harm of yellow-wild rice borers to the aquilaria sinensis. Therefore, the yellow-eared moth is a key research object for pest control in aquilaria sinensis planting areas in China at present.
Until now, there are few studies on yellow-striped rice borers. In China, only the biological and ecological characteristics and prevention and control of yellow-eared moth on aquilaria sinensis in forests are reported, and the research report of indoor artificial feeding and subculture propagation is still blank.
Disclosure of Invention
The invention aims to provide an indoor yellow-striped rice borer breeding and subculture propagation method with strong operability and stable effect, which carries out breeding propagation on yellow-striped rice borers through indoor manual work and carries out systematic research on morphological characteristics, biological characteristics and occurrence rules of the yellow-striped rice borers so as to deepen the understanding of the leaf-eating pests and provide scientific basis for the prevention and treatment of the yellow-striped rice borers.
In order to achieve the purpose, the invention provides an indoor culture and subculture propagation method for yellow wild borers, which comprises the following steps:
(1) indoor yellow wild rice borer breeding: collecting larvae of yellow-tail borer in the wild, and culturing the larvae indoors, wherein the used feed is fresh aquilaria sinensis leaves subjected to moisturizing treatment; the pupa stage is provided with loose and wet clean sandy soil after sterilization;
(2) indoor secondary propagation of yellow-eared rice stem borers: and carrying out male-female pairing by utilizing the successfully eclosion adult yellow-eared moths cultured indoors, and carrying out subculture propagation breeding on the larvae hatched after the mating female moths lay eggs.
In the step (1), wild yellow-striped rice borer larvae are collected in the field and are placed in insect-raising boxes of 16cm multiplied by 22cm multiplied by 9cm for artificial feeding, 10 larvae are placed in each box, an insect-climbing net is placed in each insect-raising box, aquilaria sinensis branch tender leaves subjected to wet cotton ball moisturizing are placed on the net, fresh aquilaria sinensis branch tender leaves are replaced in time according to the leaf eating amount of the yellow-striped rice borer larvae in different stages, the air humidity of 70% in an indoor breeding environment is ensured, and excrement of the yellow-striped rice borers is cleaned in time; when the old larva of the yellow-eared moth enters the pupa preportification, sterilized wet clean sandy soil is prepared in advance for the yellow-eared moth to penetrate into a pupa building chamber.
In the step (2), when the yellow-eared moth larvae are cultivated indoors to 5-year-old mature larvae, putting every 30 mature larvae into a previously prepared insect breeding box, wherein the height of sterilized wet clean sandy soil put into the box is 4cm, so that the yellow-eared moth larvae can conveniently enter into a pupa building room; placing the insect breeding box into an insect breeding net cage with the length of 35cm multiplied by 35cm, and eclosing adult insects after 12-13 days; preparing a batch of aquilaria sinensis seedlings with the height of 100cm in advance, sleeving a sleeve cage on the treetops of the aquilaria sinensis seedlings, putting the emerged adult yellow-tail borers into the cage according to the proportion of 30 heads and half of males and females of each sleeve cage for cage culture, and putting cotton balls in the cages to absorb 10% of honey water; the female and male adults can be copulated within 2-3 days generally, and lay eggs on the back of tender leaves of the aquilaria sinensis treetop in a cage, wherein each egg block has dozens to hundreds of eggs which are arranged in a fish scale shape; the egg laid for the first time is milk white, then gradually turns yellow, and is black before being close to hatching; the average length of the newly hatched larvae is 1.32mm, the heads of the newly hatched larvae are black and the bodies of the newly hatched larvae are light yellow, the newly hatched larvae are clustered at tender leaves of aquilaria sinensis to gnaw on the surface layers of the leaves, the whole branches with the larvae are cut off by scissors and are placed into an insect breeding box prepared in advance, meanwhile, fresh branches of the aquilaria sinensis which is subjected to cotton ball moisture preservation are placed, and when the larvae of the yellow-wild borers gnaw and eat the original tender leaves, the larvae are automatically transferred to new leaves to continue to gnaw; when the low-age larvae enter a binge eating period of 3-4 years, 30 larvae can be separately fed in each box.
The significance of the invention is as follows:
the research on the indoor culture and subculture propagation technology of the yellow-eared rice stem borers aims at the technical bottleneck that the yellow-eared rice stem borers are low in indoor culture survival rate and difficult to subculture, and research and discussion are carried out on the yellow-eared rice stem borers. Through continuous groping and breeding experiments, a set of natural ecology-simulated yellow-eared stem borer indoor breeding technology is researched, the survival rate of yellow-eared stem borer indoor breeding is greatly improved, the problem of difficult subculture of indoor breeding is solved, and continuous subculture propagation can be smoothly realized indoors, so that a stable insect source is provided for the biological control research of the yellow-eared stem borer, a basis is provided for the biological control application of the yellow-eared stem borer, and the method has important significance for realizing effective biological control of aquilaria sinensis, protecting forest ecology and human living environment, protecting biological diversity and the like.
Detailed Description
The present invention is further illustrated by the following examples.
Example (b):
indoor yellow wild rice borer breeding: collecting larvae of yellow-striped rice borers in the field, placing the larvae in insect-raising boxes of 16cm multiplied by 22cm multiplied by 9cm for artificial feeding, placing 10 larvae in each box, placing an insect-climbing net in each insect-raising box, placing aquilaria sinensis branch tender leaves subjected to moisture preservation by wet cotton balls on the net, replacing fresh aquilaria sinensis branch tender leaves in time according to the leaf feeding amount of the yellow-striped rice borers in different stages, ensuring 70% air humidity in an indoor culture environment, and cleaning excrement discharged by the yellow-striped rice borers in time; when the old larva of the yellow-eared moth enters the pupa preportification, sterilized wet clean sandy soil is prepared in advance for the yellow-eared moth to penetrate into a pupa building chamber.
Indoor secondary propagation of yellow-eared rice stem borers: when the yellow-striped rice borer larvae are cultivated indoors to 5-year-old larvae, putting every 30-head old larvae into an insect breeding box prepared in advance, wherein sterilized wet clean sandy soil put into the box is 4cm high, so that the yellow-striped rice borer larvae can conveniently enter a pupa building chamber; placing the insect breeding box into an insect breeding net cage with the length of 35cm multiplied by 35cm, and eclosing adult insects after 12-13 days; preparing a batch of aquilaria sinensis seedlings with the height of 100cm in advance, sleeving a sleeve cage on the treetops of the aquilaria sinensis seedlings, putting the emerged adult yellow-tail borers into the cage according to the proportion of 30 heads and half of males and females of each sleeve cage for cage culture, and putting cotton balls in the cages to absorb 10% of honey water; the female and male adults can be copulated within 2-3 days generally, and lay eggs on the back of tender leaves of the aquilaria sinensis treetop in a cage, wherein each egg block has dozens to hundreds of eggs which are arranged in a fish scale shape; the egg laid for the first time is milk white, then gradually turns yellow, and is black before being close to hatching; the average length of the newly hatched larvae is 1.32mm, the heads of the newly hatched larvae are black and the bodies of the newly hatched larvae are light yellow, the newly hatched larvae are clustered at tender leaves of aquilaria sinensis to gnaw on the surface layers of the leaves, the whole branches with the larvae are cut off by scissors and are placed into an insect breeding box prepared in advance, meanwhile, fresh branches of the aquilaria sinensis which is subjected to cotton ball moisture preservation are placed, and when the larvae of the yellow-wild borers gnaw and eat the original tender leaves, the larvae are automatically transferred to new leaves to continue to gnaw; when the low-age larvae enter a binge eating period of 3-4 years, 30 larvae can be separately fed in each box.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (3)
1. The indoor culture and subculture propagation method for yellow wild borers is characterized by comprising the following steps:
(1) indoor yellow wild rice borer breeding: collecting larvae of yellow-tail borer in the wild, and culturing the larvae indoors, wherein the used feed is fresh aquilaria sinensis leaves subjected to moisturizing treatment; the pupa stage is provided with loose and wet clean sandy soil after sterilization;
(2) indoor secondary propagation of yellow-eared rice stem borers: and carrying out male-female pairing by utilizing the successfully eclosion adult yellow-eared moths cultured indoors, and carrying out subculture propagation breeding on the larvae hatched after the mating female moths lay eggs.
2. The indoor culture and subculture propagation method for yellow-wild borers according to claim 1, wherein in step (1), yellow-wild borer larvae are collected in the field and are placed in an insect box of 16cm × 22cm × 9cm for artificial feeding, 10 larvae per box are placed in an insect-crawling net, aquilaria sinensis branch young leaves subjected to moisture preservation by wet cotton balls are placed on the net, fresh aquilaria sinensis branch young leaves are replaced in time according to the leaf feeding amount of the yellow-wild borer larvae at different stages, the air humidity of an indoor culture environment is ensured to be 70%, and excrement of the yellow-wild borers is cleaned in time; when the old larva of the yellow-eared moth enters the pupa preportification, sterilized wet clean sandy soil is prepared in advance for the yellow-eared moth to penetrate into a pupa building chamber.
3. The indoor yellow-eared moth breeding and subculture propagation method according to claim 1, wherein in the step (2), when yellow-eared moth larvae are bred indoors to 5-year-old larvae, every 30-head old larvae are placed in an insect-raising box prepared in advance, sterilized wet clean sandy soil put in the box is 4cm high, so that the yellow-eared moth larvae can penetrate into a pupa building chamber; placing the insect breeding box into an insect breeding net cage with the length of 35cm multiplied by 35cm, and eclosing adult insects after 12-13 days; preparing a batch of aquilaria sinensis seedlings with the height of 100cm in advance, sleeving a sleeve cage on the treetops of the aquilaria sinensis seedlings, putting the emerged adult yellow-tail borers into the cage according to the proportion of 30 heads and half of males and females of each sleeve cage for cage culture, and putting cotton balls in the cages to absorb 10% of honey water; the female and male adults can be copulated within 2-3 days generally, and lay eggs on the back of tender leaves of the aquilaria sinensis treetop in a cage, wherein each egg block has dozens to hundreds of eggs which are arranged in a fish scale shape; the egg laid for the first time is milk white, then gradually turns yellow, and is black before being close to hatching; the average length of the newly hatched larvae is 1.32mm, the heads of the newly hatched larvae are black and the bodies of the newly hatched larvae are light yellow, the newly hatched larvae are clustered at tender leaves of aquilaria sinensis to gnaw on the surface layers of the leaves, the whole branches with the larvae are cut off by scissors and are placed into an insect breeding box prepared in advance, meanwhile, fresh branches of the aquilaria sinensis which is subjected to cotton ball moisture preservation are placed, and when the larvae of the yellow-wild borers gnaw and eat the original tender leaves, the larvae are automatically transferred to new leaves to continue to gnaw; when the low-age larvae enter a binge eating period of 3-4 years, 30 larvae can be separately fed in each box.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011309036.XA CN112450155A (en) | 2020-11-20 | 2020-11-20 | Indoor culture and subculture propagation method for yellow wild rice borers |
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| CN202011309036.XA CN112450155A (en) | 2020-11-20 | 2020-11-20 | Indoor culture and subculture propagation method for yellow wild rice borers |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113973780A (en) * | 2021-06-30 | 2022-01-28 | 浙江大学 | Method for artificial feeding and breeding of mulberry borers |
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2020
- 2020-11-20 CN CN202011309036.XA patent/CN112450155A/en active Pending
Non-Patent Citations (3)
| Title |
|---|
| 乔海莉等: "黄野螟生物学特性及发生规律研究", 《应用昆虫学报》 * |
| 斩秀芳: "黄野螟幼虫对土沉香寄主及化蛹基质的选择", 《中国优秀硕博士学位论文电子期刊全文数据库电子期刊（农业科技辑）》 * |
| 陈志云等: "土沉香黄野螟生物学特性研究", 《中国植保导刊》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113973780A (en) * | 2021-06-30 | 2022-01-28 | 浙江大学 | Method for artificial feeding and breeding of mulberry borers |
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