CN113080147B - Method for preventing and treating yellow leaf borers by trichogramma - Google Patents
Method for preventing and treating yellow leaf borers by trichogramma Download PDFInfo
- Publication number
- CN113080147B CN113080147B CN202110409032.7A CN202110409032A CN113080147B CN 113080147 B CN113080147 B CN 113080147B CN 202110409032 A CN202110409032 A CN 202110409032A CN 113080147 B CN113080147 B CN 113080147B
- Authority
- CN
- China
- Prior art keywords
- eggs
- moth
- rice
- wheat bran
- rice moth
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 241000256618 Trichogramma Species 0.000 title claims abstract description 37
- 238000000034 method Methods 0.000 title claims abstract description 20
- 235000013601 eggs Nutrition 0.000 claims abstract description 98
- 241000753145 Sitotroga cerealella Species 0.000 claims abstract description 76
- 238000009395 breeding Methods 0.000 claims abstract description 49
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 47
- 230000001488 breeding effect Effects 0.000 claims abstract description 32
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 22
- 241000238631 Hexapoda Species 0.000 claims abstract description 20
- 238000012258 culturing Methods 0.000 claims abstract description 15
- 235000013305 food Nutrition 0.000 claims abstract description 12
- 230000003203 everyday effect Effects 0.000 claims abstract description 11
- 238000011161 development Methods 0.000 claims abstract description 8
- 238000011081 inoculation Methods 0.000 claims abstract description 6
- 238000005507 spraying Methods 0.000 claims abstract description 6
- 241000257303 Hymenoptera Species 0.000 claims description 42
- 241000607479 Yersinia pestis Species 0.000 claims description 10
- 241000196324 Embryophyta Species 0.000 claims description 9
- 238000012216 screening Methods 0.000 claims description 9
- 230000032669 eclosion Effects 0.000 claims description 8
- 230000018109 developmental process Effects 0.000 claims description 7
- 230000003071 parasitic effect Effects 0.000 claims description 7
- 230000001680 brushing effect Effects 0.000 claims description 5
- 238000004140 cleaning Methods 0.000 claims description 5
- 210000002257 embryonic structure Anatomy 0.000 claims description 5
- 238000003306 harvesting Methods 0.000 claims description 5
- 235000012907 honey Nutrition 0.000 claims description 5
- 235000016709 nutrition Nutrition 0.000 claims description 5
- 230000035764 nutrition Effects 0.000 claims description 5
- 230000001502 supplementing effect Effects 0.000 claims description 5
- 238000010408 sweeping Methods 0.000 claims description 5
- 241000894007 species Species 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 3
- 238000009331 sowing Methods 0.000 claims description 3
- 230000017448 oviposition Effects 0.000 claims 1
- 241000353522 Earias insulana Species 0.000 abstract description 11
- 239000003205 fragrance Substances 0.000 abstract description 3
- 241000256844 Apis mellifera Species 0.000 description 28
- 210000003128 head Anatomy 0.000 description 9
- 241001533085 Aquilaria sinensis Species 0.000 description 5
- 244000007853 Sarothamnus scoparius Species 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 3
- 241000984061 Aquilaria Species 0.000 description 2
- 241000256856 Vespidae Species 0.000 description 2
- 241000255777 Lepidoptera Species 0.000 description 1
- 240000003152 Rhus chinensis Species 0.000 description 1
- 235000014220 Rhus chinensis Nutrition 0.000 description 1
- 241001534930 Thymelaeaceae Species 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000011031 large-scale manufacturing process Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 210000004894 snout Anatomy 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Toxicology (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a method for preventing yellow stem borer with deep fragrance by trichogramma, which comprises the following steps: culturing rice moth eggs: uniformly adding 0.5 kg of wheat bran into an insect breeding sieve (in 44.5 cm, 74.8 cm, 7.5 cm), uniformly scattering 1.68-1.70 ml of fresh rice moth eggs, and then placing the insect breeding sieve in an insect breeding room at 20-25 ℃ for development; step (2), water spraying and wheat bran increasing are carried out to obtain rice moth larva food: after one week of inoculation, rice moth larvae are hatched, a small amount of water is sprayed on wheat bran of the insect breeding sieve 1 time each morning and afternoon every day, and feed needs to be added in time when the rice moth larvae are short of food. The method for preventing and treating yellow stem borers by trichogramma comprises the steps of breeding rice moths, collecting eggs of the rice moths to parasitize the yellow stem borers, preparing the parasitized eggs into bee cards, and then putting the bee cards into a victim agilawood forest to achieve the purpose of preventing and treating the yellow stem borers.
Description
Technical Field
The invention belongs to the technical field of biological control of plant protection, and particularly relates to a method for controlling yellow leaf borer by trichogramma.
Background
The trichogramma is a highly practical technology, has become a natural enemy insect with longest research history, widest application range, largest control area and best control effect on the modern agriculture and forestry pest biological control, selects an intermediate host suitable for large-scale propagation of the trichogramma on the premise of large-area application of the trichogramma, generally utilizes the stem borer as the intermediate host to carry out large-scale production of the trichogramma abroad, and realizes mechanized production.
The yellow-wild borer belongs to lepidoptera and snout moth family, is a monophagous and important leaf-feeding pest which harms aquilaria sinensis, seriously influences the healthy growth of the aquilaria sinensis, often eats leaves to become bald tips when the emergence occurs, is a typical oligophagous pest, only eats a few plants such as aquilaria resinifera and sumac, and the like, is only found to damage the aquilaria sinensis at present in China, is an aquilaria plant in the Thymelaeaceae, is listed as a national second-level important protection wild plant in 1999, has high economic value, is the most important pest in the growth process of the aquilaria sinensis, generates more than 6-8 generations in the south Hainan area in 1 year, eats larvae to eat leaves and young tender branches and stems, can eat whole leaves during outbreak, and seriously influences the growth and aroma formation of trees.
The emergence of the yellow-eared moth can lead the growth of the tree body to be slow and the death of saplings to result in the growth cycle of the grown trees or the growth cycle reaching the fragrance formation standard to be prolonged, and the influence on the yield and the quality of the agilawood is great in the fragrance formation period, so that the development of the agilawood industry is restricted, and in recent years, with the large-scale artificial pure forest planting of the aquilaria sinensis, the problems of plant diseases and insect pests such as the yellow-eared moth and the like are increasingly prominent, so that the method for preventing and treating the yellow-eared moth by using the yellow-eared moth trichogramma of the yellow-eared moth is provided, and the method aims to provide reference for the comprehensive prevention and treatment of the important yellow-eared moth of the white-eared moth and the healthy development of the white-eared moth industry.
Disclosure of Invention
The invention aims to provide a method for preventing and controlling yellow leaf borer by trichogramma, which aims to solve the problems that the emergence of the existing yellow leaf borer in the background art can lead the growth of trees to be slow and the death of young trees.
In order to achieve the purpose, the invention provides the following technical scheme: a method for preventing and treating yellow leaf borer by trichogramma comprises the following steps:
culturing rice moth eggs: uniformly adding 0.5 kg of wheat bran into an insect breeding sieve (in 44.5 cm, 74.8 cm, 7.5 cm), uniformly scattering 1.68-1.70 ml of fresh rice moth eggs, and then placing the insect breeding sieve in an insect breeding room at 20-25 ℃ for development;
step (2), water spraying and wheat bran increasing are carried out to obtain rice moth larva food: after one week of inoculation, the rice moth larvae are hatched, a small amount of water is sprayed on wheat bran of the insect breeding sieve 1 time each morning and afternoon every day, and feed needs to be added in time when the rice moth larvae lack food;
pupating the rice moth: after about 45 days, the wheat bran on the insect-breeding sieve has partial small protrusions, which indicates that the rice moth larvae begin to pupate, and the mesh sieve (48 cm < 80 cm) is covered on the insect-breeding sieve;
step (4), eclosion and moth harvesting of adult rice moths: in the morning, the adult rice moth is collected into the cage by a moth collecting machine and placed on an egg collecting rack;
step (5), collecting rice moth eggs: sweeping the eggs of the cage completely by using a hair broom, cleaning scales on the eggs of the rice moths, killing embryos by using ultraviolet rays, and then storing in a refrigerator at a certain temperature;
step (6), feeding trichogramma: collecting the parasitic yellow-eyed moth egg mass of the trichogramma in the agilawood forest to obtain the species of the trichogramma dendrobii, putting the species into an incubator for breeding, and supplying 25% of honey water for supplementing nutrition at the beginning of emergence;
step (7), bee receiving: culturing newly emerged trichogramma in an incubator with a tray uniformly sown with the eggs of the rice moth;
step (8), taking out dark cultured worm eggs: after the rice moth eggs are parasitized by breeding bees in the bee breeding box for 48 hours, taking out the eggs and the supporting plate, slightly brushing the eggs adhered on the supporting plate into a new tray by using a brush, putting the tray into an environment with the temperature of 25 +/-1 ℃ and the relative humidity of 75 +/-5 percent, culturing for 2 days, judging whether the eggs are parasitized according to the color of the eggs, wherein the eggs are black, namely the eggs are parasitized, and ventilating for 20min every day;
step (9), screening worm eggs: screening parasitic ova, and making bee cards with the ova;
step (10), bee releasing: and (4) releasing the wasps at the beginning of the egg-starting period of the yellow-eared moths, and releasing the wasps in the evening under the condition of breeze and no rain.
Preferably, in the step (2), when the wheat bran is used as a feed, the wheat bran needs to be sterilized at a high temperature of 100 ℃ for 20 minutes before the rice moth is raised, part of pests in the wheat bran are killed, water is added into the wheat bran, the mixture is uniformly stirred, and the humidity in the wheat bran is about 10%.
Preferably, the feeding conditions in the incubator in the step (6) are as follows: temperature (25 +/-1) ° c, relative humidity 75% +/-5%, and photoperiod L/D =14h/10 h.
Preferably, the culture environment of step (7) is: a dark room at 25 ℃ and 75% relative humidity.
Preferably, the ratio of the inoculated bees to eggs in the step (7) is 1: 8 to 10.
Preferably, the environment for releasing the bees in the step (10) is controlled to be at a temperature of 20-29 ℃ and a relative humidity of 65-75%.
Preferably, the bee card is hung on the main stem of the agilawood plant.
Preferably, the bee releasing needs to be carried out for three times: wherein 20000 bees per hectare are placed for the first time, the second bee placing is carried out after 4-6 days, 15000 bees per hectare are placed for the second time, the third bee placing is carried out after 6-7 days, and 10000 bees per hectare are placed for the third time.
Compared with the prior art, the invention has the beneficial effects that: the method for preventing and treating yellow stem borers by trichogramma comprises the steps of breeding rice moths and collecting eggs of the rice moths to parasitize the yellow stem borers, preparing the parasitized eggs into bee cards, and then putting the bee cards into a victim agilawood forest to realize the purpose of preventing and treating the yellow stem borers:
1. the trichogramma breeding method has the advantages that trichogramma is bred artificially and bred by using the eggs of the trichogramma, and is put into a damaged agilawood forest, so that the generation of yellow stem borers can be effectively inhibited, and the damage degree of the stem borers to agilawood is reduced.
2. The stable natural enemy population is established to inhibit the generation of the yellow stem borer of the agilawood forest, so that the use of pesticides can be reduced, and the method has important significance for environmental protection.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, rather than all embodiments.
The invention is described in detail below with reference to specific examples:
example 1:
a method for preventing and treating yellow leaf borer by trichogramma comprises the following steps:
culturing rice moth eggs: uniformly adding 0.5 kg of wheat bran into an insect breeding sieve (in 44.5 cm, 74.8 cm, 7.5 cm), uniformly scattering 1.68-1.70 ml of fresh rice moth eggs, and then placing the insect breeding sieve in an insect breeding room at 20-25 ℃ for development;
spraying water and adding wheat bran to serve as food for rice moth larvae: after one week of inoculation, rice moth larvae are hatched, a small amount of water is sprayed on wheat bran of the insect-breeding sieve 1 time each morning and afternoon every day, feed needs to be added in time when the rice moth larvae lack food, the wheat bran needs to be sterilized for 20 minutes at a high temperature of 100 ℃ before the feed is added, partial pests in the wheat bran are killed, water is added into the wheat bran and the wheat bran is uniformly stirred, and the humidity in the wheat bran is about 10%;
pupating the rice moth: after about a day, the wheat bran on the insect-breeding sieve has small protrusions, which indicates that the rice moth larvae begin to pupate, and the mesh sieve (48 cm < 80 cm) is covered on the insect-breeding sieve;
step (4), eclosion and moth harvesting of adult rice moths: in the morning, the adult rice moth is collected into the cage by a moth collecting machine and placed on an egg collecting rack;
step (5), collecting rice moth eggs: sweeping the eggs of the cage completely by using a hair broom, cleaning scales on the eggs of the rice moths, killing embryos by using ultraviolet rays, and then storing in a refrigerator at a certain temperature;
step (6), feeding trichogramma: collecting yellow-eared moth eggs parasitized by trichogramma in an agilawood forest to obtain a moth yellow-eared moth bee species, wherein the placing conditions are as follows: feeding in an incubator with the temperature of 25 +/-1) DEG C, the relative humidity of 75% +/-5% and the photoperiod L/D =14h/10h, and supplying 25% of honey water for supplementing nutrition at the beginning of eclosion;
step (7), bee receiving: culturing newly emerged trichogramma in an incubator with a tray for uniformly sowing the eggs of the rice moth, placing the incubator in a dark room with the temperature of 25 ℃ and the relative humidity of 75 percent, and ensuring the ratio of the inoculated bees to the inoculated eggs to be 1: 8;
step (8), taking out dark cultured worm eggs: after the rice moth eggs are parasitized by breeding bees in the bee breeding box for 48 hours, taking out the eggs and the supporting plate, slightly brushing the eggs adhered to the supporting plate into a new tray by using a brush, culturing for 2 days in an environment with the temperature of 25 +/-1 ℃ and the relative humidity of 75 +/-5 percent, judging whether the eggs are parasitized according to the color of the eggs, wherein the eggs are black, namely the eggs are parasitized, and ventilating for 20min every day;
step (9), worm eggs are screened: screening parasitic ova, and making bee cards with the ova;
step (10), bee releasing: the method comprises the steps of starting releasing bees at the egg beginning stage of yellow-eared moths, releasing bees under the condition of releasing bees under the conditions of breeze and no rain, temperature of 20-29 ℃ and relative humidity of 65-75% in the evening, hanging bee cards on main stems of agilawood plants, releasing bees 20000 heads/hectare for the first time, releasing bees for the second time after 4-6 days, releasing bees 15000 heads/hectare for the second time, releasing bees for the third time after 6-7 days, and releasing bees 10000 heads/hectare for the third time.
Example 2:
a method for preventing and treating yellow leaf borer by trichogramma comprises the following steps:
culturing rice moth eggs: uniformly adding 0.5 kg of wheat bran into an insect breeding sieve (in 44.5 cm, 74.8 cm, 7.5 cm), uniformly scattering 1.68-1.70 ml of fresh rice moth eggs, and then placing the insect breeding sieve in an insect breeding room at 20-25 ℃ for development;
step (2), water spraying and wheat bran increasing are carried out to obtain rice moth larva food: after one week of inoculation, rice moth larvae are hatched, a small amount of water is sprayed on wheat bran of the insect-breeding sieve 1 time each morning and afternoon every day, feed needs to be added in time when the rice moth larvae lack food, the wheat bran needs to be sterilized for 20 minutes at a high temperature of 100 ℃ before the feed is added, partial pests in the wheat bran are killed, water is added into the wheat bran and the wheat bran is uniformly stirred, and the humidity in the wheat bran is about 10%;
pupating the rice moth: after about a day, the wheat bran on the insect-breeding sieve has small protrusions, which indicates that the rice moth larvae begin to pupate, and the mesh sieve (48 cm < 80 cm) is covered on the insect-breeding sieve;
step (4), eclosion and moth harvesting of adult rice moths: in the morning, the adult rice moth is collected into the cage by a moth collecting machine and placed on an egg collecting rack;
step (5), collecting rice moth eggs: completely sweeping eggs in the cage by using a hair broom, cleaning scales on the eggs of the rice moths, killing embryos by using ultraviolet rays, and then storing in a refrigerator at a certain temperature;
step (6), feeding trichogramma: collecting yellow-eared moth eggs parasitized by trichogramma in an agilawood forest to obtain a moth yellow-eared moth bee species, wherein the placing conditions are as follows: feeding in an incubator with the temperature of 25 +/-1) DEG C, the relative humidity of 75% +/-5% and the photoperiod L/D =14h/10h, and supplying 25% of honey water for supplementing nutrition at the beginning of eclosion;
step (7), bee receiving: culturing newly emerged trichogramma in an incubator with a tray for uniformly sowing the eggs of the rice moth, placing the incubator in a dark room with the temperature of 25 ℃ and the relative humidity of 75 percent, and ensuring the ratio of the inoculated bees to the inoculated eggs to be 1: 9;
step (8), taking out dark cultured worm eggs: after the rice moth eggs are parasitized by breeding bees in the bee breeding box for 48 hours, taking out the eggs and the supporting plate, slightly brushing the eggs adhered to the supporting plate into a new tray by using a brush, culturing for 2 days in an environment with the temperature of 25 +/-1 ℃ and the relative humidity of 75 +/-5 percent, judging whether the eggs are parasitized according to the color of the eggs, wherein the eggs are black, namely the eggs are parasitized, and ventilating for 20min every day;
step (9), screening worm eggs: screening parasitic ova, and making bee cards with the ova;
step (10), bee releasing: the method comprises the steps of starting releasing bees at the beginning of oviposite period of yellow-eared moths, selecting a condition of releasing bees at the evening under the conditions of breeze and no rain, the temperature of 20-29 ℃ and the relative humidity of 65-75%, hanging bee cards on stems of agilawood plants, releasing bees 20000 heads/hectare for the first time, releasing bees at the second time after 4-6 days, releasing bees 15000 heads/hectare for the second time, releasing bees at the third time after 6-7 days, and releasing bees at 10000 heads/hectare for the third time.
Example 3:
a method for preventing and treating yellow leaf borer by trichogramma comprises the following steps:
culturing rice moth eggs: uniformly adding 0.5 kg of wheat bran into an insect breeding sieve (in 44.5 cm, 74.8 cm, 7.5 cm), uniformly scattering 1.68-1.70 ml of fresh rice moth eggs, and then placing the insect breeding sieve in an insect breeding room at 20-25 ℃ for development;
step (2), water spraying and wheat bran increasing are carried out to obtain rice moth larva food: after one week of inoculation, the rice moth larvae are hatched, a small amount of water is sprayed on wheat bran of the insect-breeding sieve respectively 1 time in the morning and afternoon every day, when the rice moth larvae lack food, feed needs to be added in time, before the feed is added, the wheat bran needs to be disinfected for 20 minutes at a high temperature of 100 ℃, part of pests in the wheat bran are killed, water is added into the wheat bran, the mixture is stirred uniformly, and the humidity in the wheat bran is about 10%;
pupating the rice moth: after about a day, the wheat bran on the insect-breeding sieve has small protrusions, which indicates that the rice moth larvae begin to pupate, and the mesh sieve (48 cm, X80 cm) is covered on the insect-breeding sieve;
step (4), eclosion and moth harvesting of adult rice moths: in the morning, the adult rice moth is collected into the cage by a moth collecting machine and placed on an egg collecting rack;
and (5) collecting rice moth eggs: sweeping the eggs of the cage completely by using a hair broom, cleaning scales on the eggs of the rice moths, killing embryos by using ultraviolet rays, and then storing in a refrigerator at a certain temperature;
step (6), feeding trichogramma: collecting yellow-eared moth eggs parasitized by trichogramma in an agilawood forest to obtain a moth yellow-eared moth bee species, wherein the placing conditions are as follows: feeding in an incubator with the temperature of 25 +/-1) DEG C, the relative humidity of 75% +/-5% and the photoperiod L/D =14h/10h, and supplying 25% of honey water for supplementing nutrition at the beginning of eclosion;
step (7), bee receiving: placing newly emerged trichogramma into an incubator with a tray for uniformly spreading eggs of the rice moth, culturing, placing the incubator into a dark room with the temperature of 25 ℃ and the relative humidity of 75%, wherein the ratio of the inoculated bees to the inoculated eggs is 1: 10;
step (8), taking out dark cultured worm eggs: after the rice moth eggs are parasitized by breeding bees in the bee breeding box for 48 hours, taking out the eggs and the supporting plate, gently brushing the eggs adhered to the supporting plate into a new tray by using a brush, placing the tray in an environment with the temperature of 25 +/-1 ℃ and the relative humidity of 75 +/-5% for culturing for 2 days, judging whether the eggs are parasitized according to the color of the eggs, wherein the eggs are black, namely the eggs are parasitized, and ventilating for 20 minutes every day;
step (9), screening worm eggs: screening parasitic ova, and making bee cards with the ova;
step (10), bee releasing: the method comprises the steps of starting releasing bees at the beginning of oviposite period of yellow-eared moths, selecting a condition of releasing bees at the evening under the conditions of breeze and no rain, the temperature of 20-29 ℃ and the relative humidity of 65-75%, hanging bee cards on stems of agilawood plants, releasing bees 20000 heads/hectare for the first time, releasing bees at the second time after 4-6 days, releasing bees 15000 heads/hectare for the second time, releasing bees at the third time after 6-7 days, and releasing bees at 10000 heads/hectare for the third time.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (1)
1. A method for preventing and treating yellow leaf borer by trichogramma is characterized by comprising the following steps:
culturing rice moth eggs: uniformly adding 0.5 kg of wheat bran into an insect-breeding sieve with the size of 44.5 cm, 74.8 cm, 7.5 cm, uniformly scattering 1.68-1.70 ml of fresh rice moth eggs, and then placing the insect-breeding sieve in an insect-breeding room at 20-25 ℃ for development;
step (2), water spraying and wheat bran increasing are carried out to obtain rice moth larva food: after one week of inoculation, the rice moth larvae are hatched, a small amount of water is sprayed on wheat bran of the insect breeding sieve 1 time each morning and afternoon every day, and feed needs to be added in time when the rice moth larvae lack food;
pupating the rice moth: after about 45 days, the wheat bran on the insect-breeding sieve has partial small protrusions, which indicates that the rice moth larvae begin to pupate, and the mesh sieve with the size of 48 cm < x > 80 cm is covered on the insect-breeding sieve;
step (4), eclosion and moth harvesting of adult rice moths: in the morning, the adult rice moth is collected into the cage by a moth collecting machine and placed on an egg collecting rack;
step (5), collecting rice moth eggs: sweeping the eggs of the cage by using a brush, cleaning scales on the eggs of the rice moths, killing embryos by using ultraviolet rays, and then storing in a refrigerator;
step (6), feeding trichogramma: collecting the parasitic yellow-eyed moth egg mass of the trichogramma in the agilawood forest to obtain the species of the trichogramma dendrobii, putting the species into an incubator for breeding, and supplying 25% of honey water for supplementing nutrition at the beginning of emergence;
step (7), bee receiving: placing newly emerged trichogramma into an incubator with a tray for uniformly sowing the eggs of the rice moth for culture;
step (8), taking out dark cultured worm eggs: after the rice moth eggs are parasitized by breeding bees in the bee breeding box for 48 hours, taking out the eggs and the tray, slightly brushing the eggs stuck on the tray into a new tray by using a brush, culturing for 2 days in an environment with the temperature of 25 +/-1 ℃ and the relative humidity of 75 +/-5 percent, judging whether the eggs are parasitized according to the color of the eggs, wherein the eggs are black, namely the eggs are parasitized, and ventilating for 20min every day;
step (9), screening worm eggs: screening parasitic ova, and making bee cards with the ova;
step (10), bee releasing: the method comprises the following steps of (1) starting releasing bees at the beginning of egg laying period of the yellow-eared moth, and releasing bees under the condition of breeze and no rain in the evening;
when the wheat bran is used as the feed in the step (2), before the rice moth is raised, the wheat bran needs to be sterilized for 20 minutes at a high temperature of 100 ℃ to kill part of pests in the wheat bran, water is added into the wheat bran and the mixture is uniformly stirred, and the humidity of the wheat bran is about 10%; the feeding conditions in the incubator in the step (6) are as follows: the temperature is 25 +/-1 ℃, the relative humidity is 75% +/-5%, and the photoperiod L/D is 14h/10 h; the culture environment of the step (7) is as follows: a dark room at 25 ℃ and 75% relative humidity; the ratio of the bees inoculated in the step (7) to the eggs is 1: 8-10; the environment for releasing the bees in the step (10) is controlled to be 20-29 ℃, and the relative humidity is 65-75%; the bee card is hung on the main stem of the agilawood plant; the bee releasing needs to be carried out for three times: wherein 20000 bees per hectare are placed for the first time, the second bee placing is carried out after 4-6 days, 15000 bees per hectare are placed for the second time, the third bee placing is carried out after 6-7 days, and 10000 bees per hectare are placed for the third time.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110409032.7A CN113080147B (en) | 2021-04-16 | 2021-04-16 | Method for preventing and treating yellow leaf borers by trichogramma |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110409032.7A CN113080147B (en) | 2021-04-16 | 2021-04-16 | Method for preventing and treating yellow leaf borers by trichogramma |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113080147A CN113080147A (en) | 2021-07-09 |
| CN113080147B true CN113080147B (en) | 2022-08-26 |
Family
ID=76677949
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110409032.7A Active CN113080147B (en) | 2021-04-16 | 2021-04-16 | Method for preventing and treating yellow leaf borers by trichogramma |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113080147B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113575238A (en) * | 2021-08-23 | 2021-11-02 | 茂名市乾圣森林绿化有限公司 | Comprehensive control method for yellow leaf borers in yellow color of deep soil |
| CN113940316A (en) * | 2021-10-20 | 2022-01-18 | 茂名市林业科学研究所 | Method for preventing and treating yellow stem borers by using trichogramma guangdongensis |
| CN114271134A (en) * | 2021-12-15 | 2022-04-05 | 广西壮族自治区林业科学研究院 | Method for preventing and controlling yellow leaf moth, soil agilawood pest, by using borer yellow trichogramma and application |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110447605A (en) * | 2019-09-17 | 2019-11-15 | 潍坊市烟草专卖局 | A kind of efficient propagation method of Trichogramma chilonis |
| CN111011313B (en) * | 2019-12-30 | 2022-04-19 | 中国医学科学院药用植物研究所云南分所 | Method for improving female proportion of trichogramma offspring and application thereof |
-
2021
- 2021-04-16 CN CN202110409032.7A patent/CN113080147B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN113080147A (en) | 2021-07-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN113080147B (en) | Method for preventing and treating yellow leaf borers by trichogramma | |
| CN103891659B (en) | Method for breeding Odontobutis obscura through fish-rice symbiosis in pool | |
| CN101796940A (en) | Super flour weevil culture technology | |
| CN103385220A (en) | Scale breeding method for encarsia formosa | |
| CN106962239A (en) | A kind of ecological three-dimensional aquaculture method | |
| CN101595857B (en) | Artificial large-scale feeding method of ophraella communa lesage | |
| CN105165313B (en) | Aphidius gifuensis short cycle mating system | |
| CN100548116C (en) | The scale artificial culture method of Ursula butterfly | |
| Lim | Sericulture training manual | |
| CN106900538A (en) | A kind of method that the onion production of hybrid seeds is carried out using fly | |
| CN107996477A (en) | A kind of lobster cultivation method based on space lotus | |
| CN108935341B (en) | Method for raising cabbage butterfly velvet cocoon bee | |
| CN112136730A (en) | Method for breeding river snails and spotted silver carps by intercropping rice shrimps | |
| CN104304133B (en) | A kind of method of mandarin fish anti-season cultivation | |
| CN113558014B (en) | Large-scale production method of dormitory lice flea bees | |
| CN100548117C (en) | The scale artificial culture technology of white butterfly | |
| CN104920318A (en) | Control method of sugarcane borers | |
| CN104381169A (en) | Culturing method of squaliobarbus curriculus regarded as forage fish for out-of-season culturing of mandarin fish | |
| CN112471082B (en) | Broadband preferred aphid breeding method | |
| CN101960999B (en) | Method for preserving ophraella communa in winter and raising ophraella communa in plastic tent | |
| CN104823923B (en) | A kind of mating system for preventing aphidius gifuensis population deterioration | |
| CN109197786B (en) | Method for breeding large amount of semi-closed curvicauda curvularia indoors | |
| CN112450155A (en) | Indoor culture and subculture propagation method for yellow wild rice borers | |
| CN113261519A (en) | Health-preserving state efficient breeding method for river crab goldfish | |
| CN111034684A (en) | Semitrella semipenguii seed conservation and rejuvenation breeding method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |