CN111543396A - Indoor large-scale breeding method for small flies and pupa terracotta warriors - Google Patents
Indoor large-scale breeding method for small flies and pupa terracotta warriors Download PDFInfo
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- CN111543396A CN111543396A CN202010566209.XA CN202010566209A CN111543396A CN 111543396 A CN111543396 A CN 111543396A CN 202010566209 A CN202010566209 A CN 202010566209A CN 111543396 A CN111543396 A CN 111543396A
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- 230000001488 breeding effect Effects 0.000 claims abstract description 42
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K20/00—Accessory food factors for animal feeding-stuffs
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- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
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- A23K20/163—Sugars; Polysaccharides
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- A—HUMAN NECESSITIES
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- A23K20/20—Inorganic substances, e.g. oligoelements
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
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Abstract
The invention relates to the technical field of insect feeding, and particularly discloses an indoor large-scale breeding method of fly pupa puppets, which comprises the following steps: collecting insect sources of bactrocera dorsalis and feeding imagoes; collecting bactrocera dorsalis eggs; feeding bactrocera dorsalis larvae; managing bactrocera dorsalis pupae; breeding the fly pupa puppets, and breeding the feathered fly pupa puppets in a bee cage for 2-3 days for later use; placing the bactrocera dorsalis pupae into a bee-keeping cage for inoculating bees; separating the parasitic pupae, and carrying out eclosion to obtain the fly pupa puppet wasp. The indoor large-scale breeding method of the fly-pupa puppet bees is simple and easy to implement, small in breeding space and low in cost, is suitable for indoor large-scale breeding of the fly-pupa puppet bees, can be used for continuous breeding to obtain a large number of the fly-pupa puppet bees, can meet experimental research on one hand, and can be used in orchards or vegetable gardens with serious damage to fly pests on the other hand to achieve biological control of the fly pests.
Description
Technical Field
The invention belongs to the technical field of insect feeding, and particularly relates to an indoor large-scale breeding method of fly pupa puppets.
Background
The Ceratoptera Mirabilis of Musca genus belongs to Hymenoptera, Ceratopteridae, and is a pupal stage single parasitic wasp. He can parasitize insects of many families of the order Diptera, including the families of anthomyzidae, Californidae, Muscaidae, Sarcophagidae, and Sarcophagidae, among others. In 1839 years, the wasp of the fly-pupa puppet is firstly reported as a parasitic wasp of the home fly pupa, besides, the wasp of the fly-pupa puppet also has a good control effect on some important fly pests, such as bactrocera dorsalis, psidium guajave flies, Indian line fruit flies and stable stabbing flies, in recent years, the wasp of the fly-pupa puppet is found to be the dominant parasitic wasp species in the pupal period of the bactrocera dorsalis, and the wasp of the fly-pupa puppet has a good control effect on the bactrocera dorsalis. The bactrocera dorsalis is an important pest of melons and fruits plants distributed worldwide, can eat and harm more than 300 kinds of cultivated fruit and vegetable crops and wild plants, has the characteristics of wide hosts, quick propagation, hidden harm, overlapping generations and the like, and causes great harm and economic loss to agricultural production and agricultural product trade. At present, the prevention and control of the bactrocera dorsalis are mainly realized by applying a large amount of chemical pesticides, and the long-term adoption of the chemical pesticides can cause the serious problems of the increased drug resistance of the bactrocera dorsalis, the increase of pesticide residue in fruits and vegetables, the gradual serious environmental pollution and the like. Biological control is an economical, safe, continuous and effective method for controlling bactrocera dorsalis. The pupation positions of the fly pests in nature are often hidden, and the fly pupae commissioned wasps have strong soil penetration capacity and can find the pupation positions of the fly pests more easily; in addition, the fly pupa commissioned wasp has strong capability of attaching to the surface of the pupa and has strong parasitic capability on the pupa of the fly pests, so that the fly pupa commissioned wasp has more remarkable advantages in controlling the fly pests. The development of the indoor large-scale breeding technology of the small bees of the fly-pupa puppets is of great significance for effectively utilizing the small bees of the fly-pupa puppets to carry out biological pest control.
Disclosure of Invention
The invention aims to provide an indoor large-scale breeding method of the fly pupa puppet wasps, which is simple and feasible and can be used for breeding the fly pupa puppet wasps on a large scale.
In order to realize the purpose, the invention provides an indoor large-scale breeding method of fly pupa puppets, which comprises the following steps:
(1) collecting the insect source of the bactrocera dorsalis and feeding imagoes: collecting the fallen fruit with insects damaged by Bactrocera dorsalis in the field, and pupating and eclosion the fallen fruit to obtain imagoes; transferring the citrus fruit fly imagoes emerging for 2 days continuously into an insect raising cage for raising, wherein the raising density is 2-2.5 ten thousand per cubic meter, imagoes nutrients and clear water are provided in the insect raising cage, and the raising conditions are as follows: the temperature is 26 +/-1 ℃, the humidity is 60-70%, and the photoperiod L: D is 14: 10;
(2) collecting bactrocera dorsalis eggs: placing the egg taking cup or the fruit into an insect rearing cage for the bactrocera dorsalis at 8: 00-9: 00 a day in the egg laying peak period of the bactrocera dorsalis, and taking out the egg taking cup or the fruit after 8-10 h to obtain the egg taking cup or the fruit containing the bactrocera dorsalis eggs;
(3) feeding of bactrocera dorsalis larvae: collecting bactrocera dorsalis eggs in an egg taking cup, putting the bactrocera dorsalis eggs into an insect raising box filled with larva feed, uniformly spreading the eggs, and incubating the eggs into larvae for raising; or putting the fruits containing the bactrocera dorsalis eggs into an insect breeding box filled with larva feed for breeding; the breeding conditions are as follows: the temperature is 26 +/-1 ℃, and the humidity is 60-70%;
(4) managing bactrocera dorsalis pupae: after feeding the bactrocera dorsalis larvae to 5-6 days old, placing the feeding tray in a pupation box with the bottom paved with high-temperature sterilized wet sand with the thickness of 4-5 cm, jumping the aged larvae into the wet sand for pupation, collecting the bactrocera dorsalis pupae after pupation, and uniformly paving the bactrocera dorsalis pupae in a bee receiving tray with the bottom filled with wet fine sand for later use;
(5) breeding the fly pupa puppets: placing the feathered small bee of the fly pupa puppet in a bee-keeping cage for breeding for 2-3 days, wherein the number ratio of the male bee to the female bee of the fly pupa puppet is 1: 1-2, honey water with the concentration of 15% and clear water are provided in the bee-keeping cage, the temperature in the bee-keeping cage is kept at 26 +/-1 ℃, the humidity is 60-70%, and the photoperiod L: D is 14: 10;
(6) bee inoculation: placing the bee-receiving tray filled with the small fruit fly pupae in the step (4) into the bee-keeping cage in the step (5) for the small bee of the fly pupae to parasitize and lay eggs, and replacing a new bee-receiving tray after 20-24 hours;
(7) breeding the fly pupa puppets: and (3) placing the bee receiving disc after bee receiving into a pupation box, allowing non-parasitic pupae to emerge into Bactrocera dorsalis within 10-12 days, collecting the residual un-emerged pupae as parasitic pupae, moving the parasitic pupae into a bee-keeping cage, and allowing the parasitic pupae to emerge after 7-8 days to obtain the bee pupae of the fly figurines.
Preferably, in the indoor large-scale breeding method of the fly pupa puppet bees, the insect cage is made of an aluminum pipe frame and a 120-mesh gauze, a circular opening is formed in one side face of the insect cage, and a gauze oversleeve is connected to the circular opening; a feeding groove and a water feeding groove are formed in the insect breeding cage, and the feeding groove is used for placing adult nutrients; the improved water feeding device is characterized in that a cover body is arranged on the water feeding groove, one layer of absorbent cotton is laid above the cover body, a through hole is formed in the cover body, a water absorbing cotton core is arranged in the through hole, one end of the water absorbing cotton core extends to the bottom of the water feeding groove, and the other end of the water absorbing cotton core extends out of the through hole to be connected with the absorbent cotton. The absorbent cotton core adsorbs water from the water feeding groove to the absorbent cotton for the bactrocera dorsalis to absorb, the absorbent cotton layer facilitates the bactrocera dorsalis to land, the bactrocera dorsalis falls into water to be drowned when water is drunk, impurities can be effectively prevented from entering the water, the water is kept clean, the pollution is reduced, and the replacement frequency is reduced.
Preferably, in the indoor large-scale breeding method of the fly pupa puppet bees, the adult nutrient is a mixture of yeast and sucrose in a weight ratio of 1: 1.
Preferably, in the indoor large-scale breeding method of the fly pupa puppet bees, in the step (3), the larva feed consists of the following raw materials in parts by weight: 280-320 parts of bananas, 250-350 parts of corn flour, 50-80 parts of yeast, 50-80 parts of cane sugar, 1.0-1.5 parts of sodium benzoate, 50-100 parts of fibers, 2.5-3.0 parts of hydrochloric acid and 300-350 parts of distilled water, wherein the raw materials are ground and fully mixed to obtain a larva feed, and the larva feed is stored at the temperature of 3-5 ℃ for later use; 100-120 g of the larva feed is prepared for every 1000 bactrocera dorsalis eggs.
Preferably, in the indoor large-scale breeding method of the fly pupa puppet wasps, the egg taking cup consists of a cup body and a cup cover, a plurality of rows of small holes are formed in the cup body, the aperture of each small hole is 1-1.5 mm, the row spacing of the small holes is 1-2 cm, the hole spacing is 1-1.5 cm, and orange juice is adhered to the inner wall of the cup body.
Preferably, in the indoor large-scale breeding method of the fly pupa puppet bees, the fruits are ripe bananas, carambola or guavas, and the surfaces of the fruits are punched or cut into small strip-shaped openings.
Preferably, in the indoor large-scale breeding method of the fly pupa puppet bees, in the step (6), the number ratio of the fly pupa puppet bees to the citrus fruit fly pupas is 1: 10-12 during the bee catching.
Compared with the prior art, the invention has the following beneficial effects:
1. the indoor large-scale breeding method of the fly-pupa puppet bees is simple and easy to implement, small in breeding space and low in cost, is suitable for indoor large-scale breeding of the fly-pupa puppet bees, can be used for continuous breeding to obtain a large number of the fly-pupa puppet bees, can meet experimental research on one hand, and can be used in orchards or vegetable gardens with serious damage to fly pests on the other hand to achieve biological control of the fly pests.
2. The indoor large-scale breeding method of the small flies and pupa puppets comprises the steps of firstly, obtaining excellent small flies and pupas of the oranges through standardized breeding of the oranges and the small flies to serve as parasitized objects, then, connecting the bees on the small flies and the pupas of the oranges and the pupas, controlling the bee connecting time, realizing the separation of the parasitized pupas and the non-parasitized pupas by utilizing the time difference of the emergence of the small flies and the pupas of the oranges and the pupas of the chrysalis. The method for breeding the fly pupa puppet has the advantages of high parasitic rate and emergence rate of the fly pupa puppet, low loss and high breeding efficiency.
Drawings
FIG. 1 is a photograph of an egg of Bactrocera dorsalis.
FIG. 2 is a photograph of a bee-catching tray on which pupae of Bactrocera dorsalis are spread.
Fig. 3 is a photograph of a parasitic pupa parasitized by a fly pupa puppet.
Detailed Description
The following detailed description of specific embodiments of the invention is provided, but it should be understood that the scope of the invention is not limited to the specific embodiments.
Example 1
An indoor large-scale breeding method of fly pupa puppets is characterized by comprising the following steps:
(1) collecting the insect source of the bactrocera dorsalis and feeding imagoes: collecting the fallen fruit with insects damaged by Bactrocera dorsalis in the field, and pupating and eclosion the fallen fruit to obtain imagoes; transferring the citrus fruit fly imagoes emerging for 2 days into an insect cage for feeding, wherein the feeding density is 2.3 ten thousand per cubic meter, the imagoes are provided with the nutrients and clear water in the insect cage, and the feeding conditions are as follows: the temperature is 26 +/-1 ℃, the humidity is 65%, and the photoperiod L: D is 14: 10; the adult nutrient is a mixture of yeast and sucrose in a weight ratio of 1: 1;
(2) collecting bactrocera dorsalis eggs: in the peak egg-laying period of the bactrocera dorsalis, bananas are placed into the bactrocera dorsalis insect rearing cage at a rate of 8: 00-9: 00 every day, and small holes are punched on the surfaces of the bananas to facilitate the bactrocera dorsalis to lay eggs; taking out the bananas after 10 hours;
(3) feeding of bactrocera dorsalis larvae: the method comprises the following steps of putting bananas containing bactrocera dorsalis eggs into an insect breeding box with larva feed for breeding, wherein the insect breeding box comprises a box body and a box cover, the middle of the box cover is provided with an opening, and the opening is connected with a 100-mesh gauze, so that the air permeability of the insect breeding box is ensured, and meanwhile, the larvae are prevented from escaping; the breeding conditions are as follows: the temperature is 26 +/-1 ℃, and the humidity is 70%; the larva feed consists of the following raw materials in parts by weight: 300 parts of bananas, 300 parts of corn flour, 60 parts of yeast, 60 parts of cane sugar, 1.2 parts of sodium benzoate, 60 parts of fiber, 2.8 parts of hydrochloric acid and 300 parts of distilled water, and the raw materials are ground and fully mixed to obtain larva feed; preparing 100g of larva feed for every 1000 bactrocera dorsalis eggs;
(4) managing bactrocera dorsalis pupae: after feeding the bactrocera dorsalis larvae to 5 days old, placing the feeding tray in a pupation box with the bottom paved with 5 cm-thick high-temperature sterilization wet sand, jumping aged larvae into the wet sand for pupation, collecting the bactrocera dorsalis pupae after pupation, and uniformly paving the bactrocera dorsalis pupae in a bee receiving tray with the bottom filled with the wet fine sand for later use;
(5) breeding the fly pupa puppets: placing the feathered puppet in a bee-keeping cage for breeding for 2 days, wherein the number ratio of the male bee to the female bee of the puppet is 1:1, honey water and clear water with the concentration of 15% are provided in the bee-keeping cage, the temperature in the bee-keeping cage is kept at 26 +/-1 ℃, the humidity is 70%, and the photoperiod L: D is 14: 10;
(6) bee inoculation: placing the bee-receiving tray in the step (4) into the bee-keeping cage in the step (5) for the small flies and pupa terracotta soldiers to parasitize and lay eggs, and replacing a new bee-receiving tray after 24 hours; the number ratio of the small flies of the fly pupa figurines to the small citrus fruit flies in the process of bee catching is 1: 10;
(7) breeding the fly pupa puppets: and (3) placing the bee receiving disc after bee receiving into a pupation box, after 12 days, enabling non-parasitic pupae to emerge into bactrocera dorsalis, collecting the residual non-emerging pupae to be parasitic pupae, separating the parasitic pupae from the non-parasitic pupae by utilizing the time difference of the emergence of the bactrocera dorsalis and the small bee of the fly pupae, moving the parasitic pupae into a bee-keeping cage, and after 7-8 days, enabling the parasitic pupae to emerge to obtain the small bee of the fly pupae.
Example 2
An indoor large-scale breeding method of fly pupa puppets is characterized by comprising the following steps:
(1) collecting the insect source of the bactrocera dorsalis and feeding imagoes: collecting the fallen fruit with insects damaged by Bactrocera dorsalis in the field, and pupating and eclosion the fallen fruit to obtain imagoes; transferring the citrus fruit fly imagoes emerging for 2 days into an insect cage for feeding, wherein the feeding density is 2.3 ten thousand per cubic meter, the imagoes are provided with the nutrients and clear water in the insect cage, and the feeding conditions are as follows: the temperature is 26 +/-1 ℃, the humidity is 65%, and the photoperiod L: D is 14: 10; the adult nutrient is a mixture of yeast and sucrose in a weight ratio of 1: 1;
(2) collecting bactrocera dorsalis eggs: placing the egg taking cup into an insect feeding cage for the bactrocera dorsalis at 8: 00-9: 00 a day in the peak egg laying period of the bactrocera dorsalis, and taking out the egg taking cup after 8 hours to obtain the egg taking cup containing the bactrocera dorsalis eggs; the egg taking cup consists of a cup body and a cup cover, wherein a plurality of rows of small holes are formed in the cup body, the aperture of each small hole is 1.0mm, the row spacing of the small holes is 1.5cm, the hole spacing is 1cm, orange juice is adhered to the inner wall of the cup body, and the bactrocera dorsalis is attracted to lay eggs;
(3) feeding of bactrocera dorsalis larvae: scouring eggs on the wall of the cup by using a watering can, filtering water by using a 120-mesh gauze, collecting the bactrocera dorsalis eggs as shown in figure 1, putting the bactrocera dorsalis eggs into an insect box paved with larva feed, uniformly paving the eggs, incubating the eggs into larvae, and feeding the larvae, wherein the feeding conditions are as follows: the temperature is 26 +/-1 ℃, and the humidity is 70%; the insect-breeding box comprises a box body and a box cover, wherein the middle of the box cover is provided with an opening, and the opening is connected with a 100-mesh gauze; the larva feed consists of the following raw materials in parts by weight: 300 parts of bananas, 300 parts of corn flour, 60 parts of yeast, 60 parts of cane sugar, 1.2 parts of sodium benzoate, 60 parts of fiber, 2.8 parts of hydrochloric acid and 300 parts of distilled water, and the raw materials are ground and fully mixed to obtain larva feed; preparing 100g of larva feed for every 1000 bactrocera dorsalis eggs;
(4) managing bactrocera dorsalis pupae: after feeding the bactrocera dorsalis larvae to 5 days old, placing the feeding tray in a pupation box with the bottom paved with 5cm wet sand subjected to high-temperature sterilization, jumping aged larvae into the wet sand for pupation, collecting the bactrocera dorsalis pupae after pupation, and uniformly paving the bactrocera dorsalis pupae in a bee receiving tray with the bottom filled with the wet fine sand as shown in figure 2 for later use;
(5) breeding the fly pupa puppets: placing the feathered puppet in a bee-keeping cage for breeding for 2 days, wherein the number ratio of the male bee to the female bee of the puppet is 1:1, honey water and clear water with the concentration of 15% are provided in the bee-keeping cage, the temperature in the bee-keeping cage is kept at 26 +/-1 ℃, the humidity is 70%, and the photoperiod L: D is 14: 10;
(6) bee inoculation: placing the bee-receiving tray in the step (4) into the bee-keeping cage in the step (5) for the small flies and pupa terracotta soldiers to parasitize and lay eggs, and replacing a new bee-receiving tray after 20 hours; the number ratio of the small flies of the fly pupa figurines to the small citrus fruit flies in the process of bee catching is 1: 12;
(7) breeding the fly pupa puppets: and (3) placing the bee receiving disc after bee receiving into a pupation box, after 12 days, enabling non-parasitic pupae to emerge into Bactrocera dorsalis, collecting the residual un-emerged pupae as parasitic pupae as shown in figure 3, moving the parasitic pupae into a bee-keeping cage, and after 7-8 days, enabling the parasitic pupae to emerge to obtain the fly pupae puppet wasp.
The insect cage is made of an aluminum tube frame and 120-mesh gauze, a round opening is formed in one side face of the insect cage, and a gauze oversleeve is connected to the round opening, so that clean water and feed can be conveniently replaced; a feeding groove and a water feeding groove are arranged in the insect cage, and the feeding groove is used for placing adult nutrients; the water feeding tank is provided with a cover body, a layer of absorbent cotton is laid above the cover body, the cover body is provided with a through hole, a water absorbing cotton core is arranged in the through hole, one end of the water absorbing cotton core extends to the bottom of the water feeding tank, and the other end of the water absorbing cotton core extends out of the through hole to be connected with the absorbent cotton. The absorbent cotton core adsorbs water from the water feeding groove to the absorbent cotton for the bactrocera dorsalis to absorb, the absorbent cotton layer facilitates the bactrocera dorsalis to land, the bactrocera dorsalis falls into water to be drowned when water is drunk, impurities can be effectively prevented from entering the water, the water is kept clean, the pollution is reduced, and the replacement frequency is reduced.
Indoor breeding test for fly pupa figurines and wasps
A test insect source: the bactrocera dorsalis insect source is that carambola, guava or mango with insects damaged by the bactrocera dorsalis are collected in the field and pupated and eclosized indoors to obtain imagoes. The source of the bee species of the fly pupa puppets is as follows: and 12 months in 2019, introducing a feeding population of Guangdong provincial academy of agricultural sciences, and breeding indoors to establish a stable experimental population.
The test method comprises the following steps: respectively setting an embodiment 1-2 group and a comparison 1-2 group, wherein the embodiment 1-2 group adopts the methods provided by the embodiment 1 and the embodiment 2 to breed the fly pupa puppets respectively; the group bee time of comparison 1 is 18h, and other parameters and method steps are the same as those of example 1; comparative 2 group bee time was 26h, other parameters and method steps were the same as in example 1. The specification of the bee-keeping cages is 35 multiplied by 35cm, one cage is a group, 50 pairs of male and female bees of the fly pupa puppets are raised in each cage, each group is repeated for 3 times, the parasitism situation of the fly pupa puppets of each group to the small fruit fly pupae of the oranges and the emergence situation of the parasitism pupae are observed, and the parasitism rate and the emergence rate of the fly pupa puppets are calculated.
And (3) test results: table 1 shows the parasitic rate and the emergence rate of the puppet bees of the fly pupas of each test group, and it can be known from the table that the parasitic rate and the emergence rate of the puppet bees of the fly pupas bred by the method provided by the embodiment of the invention are relatively high, the parasitic rate is relatively low when the fly pupa puppet bee receiving time is too short (compared with 1 group), the emergence rate of the fly pupa puppet bees is obviously low when the fly pupa puppet bee receiving time is too long (compared with 2 groups), the phenomenon of uneven emergence can occur when the parasitic time is too long, the emergence uniformity of the fly pupa puppet bees is influenced, and the later-stage breeding management is not convenient. The invention provides an indoor large-scale breeding method of the fly pupa puppet wasps, which effectively improves the parasitic rate and the emergence rate of the fly pupa puppet wasps and can carry out large-scale breeding on the fly pupa puppet wasps.
TABLE 1 parasitic rate and emergence rate of the fly pupa puppets of each test group
| Group of | Percentage of parasitism% | Eclosion rate% |
| EXAMPLE 1 group | 74.8 | 89.5 |
| EXAMPLE 2 group | 76.3 | 90.8 |
| Comparative 1 group | 60.3 | 88.3 |
| Comparative 2 group | 74.5 | 64.1 |
The foregoing descriptions of specific exemplary embodiments of the present invention have been presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain certain principles of the invention and its practical application to enable one skilled in the art to make and use various exemplary embodiments of the invention and various alternatives and modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.
Claims (7)
1. An indoor large-scale breeding method of fly pupa puppets is characterized by comprising the following steps:
(1) collecting the insect source of the bactrocera dorsalis and feeding imagoes: collecting the fallen fruit with insects damaged by Bactrocera dorsalis in the field, and pupating and eclosion the fallen fruit to obtain imagoes; transferring the citrus fruit fly imagoes emerging for 2 days continuously into an insect raising cage for raising, wherein the raising density is 2-2.5 ten thousand per cubic meter, imagoes nutrients and clear water are provided in the insect raising cage, and the raising conditions are as follows: the temperature is 26 +/-1 ℃, the humidity is 60-70%, and the photoperiod L: D is 14: 10;
(2) collecting bactrocera dorsalis eggs: placing the egg taking cup or the fruit into an insect rearing cage for the bactrocera dorsalis at 8: 00-9: 00 a day in the egg laying peak period of the bactrocera dorsalis, and taking out the egg taking cup or the fruit after 8-10 h to obtain the egg taking cup or the fruit containing the bactrocera dorsalis eggs;
(3) feeding of bactrocera dorsalis larvae: collecting bactrocera dorsalis eggs in an egg taking cup, putting the bactrocera dorsalis eggs into an insect raising box filled with larva feed, uniformly spreading the eggs, and incubating the eggs into larvae for raising; or putting the fruits containing the bactrocera dorsalis eggs into an insect breeding box filled with larva feed for breeding; the breeding conditions are as follows: the temperature is 26 +/-1 ℃, and the humidity is 60-70%;
(4) managing bactrocera dorsalis pupae: after feeding the bactrocera dorsalis larvae to 5-6 days old, placing the feeding tray in a pupation box with the bottom paved with high-temperature sterilized wet sand with the thickness of 4-5 cm, jumping the aged larvae into the wet sand for pupation, collecting the bactrocera dorsalis pupae after pupation, and uniformly paving the bactrocera dorsalis pupae in a bee receiving tray with the bottom filled with wet fine sand for later use;
(5) breeding the fly pupa puppets: placing the feathered small bee of the fly pupa puppet in a bee-keeping cage for breeding for 2-3 days, wherein the number ratio of the male bee to the female bee of the fly pupa puppet is 1: 1-2, honey water with the concentration of 15% and clear water are provided in the bee-keeping cage, the temperature in the bee-keeping cage is kept at 26 +/-1 ℃, the humidity is 60-70%, and the photoperiod L: D is 14: 10;
(6) bee inoculation: placing the bee-receiving tray filled with the small fruit fly pupae in the step (4) into the bee-keeping cage in the step (5) for the small bee of the fly pupae to parasitize and lay eggs, and replacing a new bee-receiving tray after 20-24 hours;
(7) breeding the fly pupa puppets: and (3) placing the bee receiving disc after bee receiving into a pupation box, allowing non-parasitic pupae to emerge into Bactrocera dorsalis within 10-12 days, collecting the residual un-emerged pupae as parasitic pupae, moving the parasitic pupae into a bee-keeping cage, and allowing the parasitic pupae to emerge after 7-8 days to obtain the bee pupae of the fly figurines.
2. The indoor large-scale breeding method of fly pupa puppets according to claim 1, wherein the insect-raising cage is made of an aluminum tube frame and 120-mesh gauze, a circular opening is opened on one side surface of the insect-raising cage, and a gauze oversleeve is connected to the circular opening; a feeding groove and a water feeding groove are formed in the insect breeding cage, and the feeding groove is used for placing adult nutrients; the improved water feeding device is characterized in that a cover body is arranged on the water feeding groove, one layer of absorbent cotton is laid above the cover body, a through hole is formed in the cover body, a water absorbing cotton core is arranged in the through hole, one end of the water absorbing cotton core extends to the bottom of the water feeding groove, and the other end of the water absorbing cotton core extends out of the through hole to be connected with the absorbent cotton.
3. The indoor large-scale breeding method of the fly pupa puppet according to claim 1, wherein the adult nutrient is a mixture of yeast and sucrose in a weight ratio of 1: 1.
4. The indoor large-scale breeding method of the fly pupa puppet according to claim 1, wherein in the step (3), the larva feed is composed of the following raw materials in parts by weight: 280-320 parts of bananas, 250-350 parts of corn flour, 50-80 parts of yeast, 50-80 parts of cane sugar, 1.0-1.5 parts of sodium benzoate, 50-100 parts of fibers, 2.5-3.0 parts of hydrochloric acid and 300-350 parts of distilled water, wherein the raw materials are ground and fully mixed to obtain a larva feed, and the larva feed is stored at the temperature of 3-5 ℃ for later use; 100-120 g of the larva feed is prepared for every 1000 bactrocera dorsalis eggs.
5. The indoor large-scale breeding method of the fly pupa puppet according to claim 1, wherein the egg taking cup is composed of a cup body and a cup cover, the cup body is provided with a plurality of rows of small holes, the diameter of each small hole is 1-1.5 mm, the row spacing of the small holes is 1-2 cm, the hole spacing is 1-1.5 cm, and orange juice is adhered to the inner wall of the cup body.
6. The indoor large-scale breeding method of the fly pupa puppet bees according to claim 1, wherein the fruits are ripe bananas, carambola fruits or guavas, and the fruits are perforated or cut into small strip-shaped openings on the surfaces of the fruits.
7. The indoor large-scale breeding method of the fly pupa puppets as claimed in claim 1, wherein in the step (6), the number ratio of the fly pupa puppets to the small fruit fly pupas during the bee catching is 1: 10-12.
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