CN110521674B - Indoor breeding method for Brachypodium banseri - Google Patents
Indoor breeding method for Brachypodium banseri Download PDFInfo
- Publication number
- CN110521674B CN110521674B CN201910791379.5A CN201910791379A CN110521674B CN 110521674 B CN110521674 B CN 110521674B CN 201910791379 A CN201910791379 A CN 201910791379A CN 110521674 B CN110521674 B CN 110521674B
- Authority
- CN
- China
- Prior art keywords
- insect
- potatoes
- mealybugs
- parasitized
- bees
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000009395 breeding Methods 0.000 title claims description 24
- 241000743774 Brachypodium Species 0.000 title claims description 20
- 241000238631 Hexapoda Species 0.000 claims abstract description 95
- 244000061456 Solanum tuberosum Species 0.000 claims abstract description 69
- 235000002595 Solanum tuberosum Nutrition 0.000 claims abstract description 69
- 235000012015 potatoes Nutrition 0.000 claims abstract description 51
- 241000257303 Hymenoptera Species 0.000 claims abstract description 30
- 238000000034 method Methods 0.000 claims abstract description 25
- 241001481304 Vespoidea Species 0.000 claims abstract description 19
- 230000009191 jumping Effects 0.000 claims abstract description 18
- 238000011160 research Methods 0.000 claims abstract description 17
- 238000012360 testing method Methods 0.000 claims abstract description 4
- 241001465977 Coccoidea Species 0.000 claims description 37
- 241000255791 Bombyx Species 0.000 claims description 30
- 230000001488 breeding effect Effects 0.000 claims description 21
- 244000284380 Hibiscus rosa sinensis Species 0.000 claims description 20
- 229920000742 Cotton Polymers 0.000 claims description 16
- 241000255990 Helicoverpa Species 0.000 claims description 16
- 239000004576 sand Substances 0.000 claims description 16
- 241001492664 Solenopsis <angiosperm> Species 0.000 claims description 15
- 230000032669 eclosion Effects 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 235000000100 Hibiscus rosa sinensis Nutrition 0.000 claims description 8
- 241001121967 Lecanicillium Species 0.000 claims description 8
- 230000003203 everyday effect Effects 0.000 claims description 7
- 235000012907 honey Nutrition 0.000 claims description 7
- 235000016709 nutrition Nutrition 0.000 claims description 7
- 230000035764 nutrition Effects 0.000 claims description 7
- 230000001502 supplementing effect Effects 0.000 claims description 7
- 241001136816 Bombus <genus> Species 0.000 claims description 6
- 244000048199 Hibiscus mutabilis Species 0.000 claims description 6
- 235000003973 Hibiscus mutabilis Nutrition 0.000 claims description 6
- 241000607479 Yersinia pestis Species 0.000 claims description 6
- 241000255789 Bombyx mori Species 0.000 claims description 3
- 238000005286 illumination Methods 0.000 claims description 2
- 238000012544 monitoring process Methods 0.000 claims description 2
- 238000012258 culturing Methods 0.000 claims 4
- 241000218993 Begonia Species 0.000 claims 1
- 241000766395 Maconellicoccus hirsutus Species 0.000 claims 1
- 241000196324 Embryophyta Species 0.000 abstract description 16
- 230000003071 parasitic effect Effects 0.000 abstract description 8
- 239000000463 material Substances 0.000 abstract description 5
- 241001674044 Blattodea Species 0.000 abstract description 2
- 240000003553 Leptospermum scoparium Species 0.000 abstract description 2
- 235000016887 Leptospermum scoparium Nutrition 0.000 abstract description 2
- 230000000052 comparative effect Effects 0.000 description 13
- 235000005206 Hibiscus Nutrition 0.000 description 12
- 235000007185 Hibiscus lunariifolius Nutrition 0.000 description 12
- 241000227653 Lycopersicon Species 0.000 description 12
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 11
- 241000219146 Gossypium Species 0.000 description 9
- 230000002265 prevention Effects 0.000 description 6
- 235000016936 Dendrocalamus strictus Nutrition 0.000 description 5
- 241000270290 Gekkota Species 0.000 description 4
- 238000011081 inoculation Methods 0.000 description 4
- 241000258937 Hemiptera Species 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 210000001320 hippocampus Anatomy 0.000 description 3
- 244000025352 Artocarpus heterophyllus Species 0.000 description 2
- 235000008725 Artocarpus heterophyllus Nutrition 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- LGZXYFMMLRYXLK-UHFFFAOYSA-N mercury(2+);sulfide Chemical compound [S-2].[Hg+2] LGZXYFMMLRYXLK-UHFFFAOYSA-N 0.000 description 2
- 230000024241 parasitism Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 240000000031 Achyranthes bidentata Species 0.000 description 1
- 241001080586 Aenasius Species 0.000 description 1
- 235000003276 Apios tuberosa Nutrition 0.000 description 1
- 102000013585 Bombesin Human genes 0.000 description 1
- 108010051479 Bombesin Proteins 0.000 description 1
- 235000007516 Chrysanthemum Nutrition 0.000 description 1
- 244000189548 Chrysanthemum x morifolium Species 0.000 description 1
- 241001391944 Commicarpus scandens Species 0.000 description 1
- 235000015001 Cucumis melo var inodorus Nutrition 0.000 description 1
- 240000002495 Cucumis melo var. inodorus Species 0.000 description 1
- 241001147399 Encyrtidae Species 0.000 description 1
- 241001466007 Heteroptera Species 0.000 description 1
- 235000002262 Lycopersicon Nutrition 0.000 description 1
- 241000819999 Nymphes Species 0.000 description 1
- 241000209270 Phenacoccus solenopsis Species 0.000 description 1
- 235000016785 Rosa della China Nutrition 0.000 description 1
- 241000256856 Vespidae Species 0.000 description 1
- 244000170226 Voandzeia subterranea Species 0.000 description 1
- 235000013030 Voandzeia subterranea Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- DNDCVAGJPBKION-DOPDSADYSA-N bombesin Chemical compound C([C@@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCSC)C(N)=O)NC(=O)CNC(=O)[C@@H](NC(=O)[C@H](C)NC(=O)[C@H](CC=1NC2=CC=CC=C2C=1)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H]1NC(=O)CC1)C(C)C)C1=CN=CN1 DNDCVAGJPBKION-DOPDSADYSA-N 0.000 description 1
- 235000012343 cottonseed oil Nutrition 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 244000037666 field crops Species 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 235000007924 ground bean Nutrition 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 235000002741 hibiscus rosa-sinensis Nutrition 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000003211 malignant effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 239000000779 smoke Substances 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Catching Or Destruction (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention relates to a raising method of Brazilian jumping bees, which breeds the Brazilian jumping bees by using germinated potatoes, and can shorten the preparation time of the early-stage materials so as to avoid the shortage of host plant materials in the test process. A lot of space is saved, i.e. a lot of parasitic bees are available in the same space. And the stiff cockroaches are easy to pick up, so that errors caused by untimely picking up of the insects after the emergence of the Brazilian jumping bees are avoided. Is particularly suitable for research or field release of a large amount of parasitic wasps to quickly control the Leptospermum scoparium.
Description
Technical Field
The invention belongs to the technical field of insect breeding, and relates to a breeding method of Brazilian jumping bees.
Background
Encyrtidae of Heteroptera of Hayat of Aenasius bambawaleii is an important parasitic natural enemy of Fusang mealybugs, is an endoparasitic bee, is firstly found in India in 2008 and is mainly distributed in China, India and Pakistan.
Phenacoccus solenopsis Tinsley belonging to Hemiptera of Faspiacaceae is a pest seriously threatening plants such as field crops, garden ornamental plants, fruit trees, vegetables and the like, is produced in North America and is a malignant invasive pest widely distributed in the world. The female adult and nymph can suck plant juice to affect plant growth, and the worm can secrete honeydew to pollute leaves, so that sooty smoke is caused, photosynthesis function of the leaves is reduced, the damaged plants grow slowly or stop, and the plants die in pieces in severe cases. For the prevention and treatment of the mealybugs of the hibiscus sinensis, the physical prevention and treatment effect and the chemical prevention and treatment effect are not ideal, and the chemical prevention and treatment can cause the problem of '3R', so the biological prevention and treatment is one of the most effective and ideal methods. In nature, the natural enemies of the Hippocampus sinensis mealybugs are more, wherein the Queshell of Banshen is the dominant natural enemy of the Hippocampus sinensis mealybugs, the natural control of the population of the Hippocampus sinensis mealybugs plays an important role, and the biological control effect on the pests is remarkable.
A series of researches on the biological characteristics, reproductive development, prevention and treatment of the Hibiscus rosaefolius Leporus are urgent. And a large number of insects with a physiological standard are required in the research. Therefore, indoor artificial subculture is a precondition for carrying out various researches to obtain a large amount of parasitic wasps. At present, hosts bred in a small bans indoor breeding house mainly comprise mealybugs on hibiscus, mealybugs on cotton seedlings, mealybugs on tomato seedlings and mealybugs on potato seedlings. However, the hibiscus, cotton, tomatoes and potato seedlings are required to be watered regularly during indoor culture, and the hibiscus, the cotton, the tomatoes and the potato seedlings are easy to wither or even die if the watering is not timely. The potato seedlings are extremely easy to dry and die in summer. Meanwhile, in the watering process, the small bombus batrachii is easy to escape, and the feeding efficiency is reduced. The invention discloses a method for indoor breeding of mealybugs and bombyx bateri, applied to Shujiang Shuishan cotton-flax research institute, and the application number is 201711286885.6, and the reproduction of the bombyx bateri is carried out by inoculating the Fumori mealybugs on the water-cultured achyranthes bidentata chrysanthemum. The method is suitable for establishing and maintaining indoor population of the Brachypodium banseri, but is not suitable for obtaining a large number of Brachypodium banseri in a short time, and the Brachypodium banseri is used as a research material or released in the field to control the Hibiscus solenopsis. A large number of small bans jumping bees are obtained in a short time, a feeding method which is simple to operate and easy to collect insects is needed, and the method has the advantages.
Disclosure of Invention
The invention aims to provide an indoor subculture method which is simple in steps and can quickly obtain a large number of Brazilian jumping bees.
In order to achieve the above object, the present invention has the following technical means.
An indoor subculture method of Brazilian jumping bees comprises the following steps:
s1, burying 100g potatoes into wet sand, enabling the tops of the potatoes to be 2cm away from the surface of a sand layer, waiting for the potatoes to sprout until the sprouts grow to 2cm, and taking out the potatoes with the sprouts from the sand for later use;
s2, transferring 25 female imagoes or third instar nymphs of the Chinese hibiscus mealybugs to the germinated potatoes;
s3, placing the germinated potatoes and female adult and third-instar nymphs of the mealybugs of the hibiscus sinensis into an insect box with the specification of 22cm multiplied by 15cm multiplied by 8cm, and placing the box on an insect support in an insect room for feeding, wherein the feeding conditions are as follows: temperature 28 ± 2 ℃, photoperiod 14L: 10D, relative humidity 80 +/-5%;
s4, placing 4 female bombyx batrachii and 2 male bombyx mori which are not mated after eclosion into a pest breeding box, enabling the bombyx batrachyces to parasitize the bombyx batrachyces for 72 hours, and placing absorbent cotton dipped with 10% of honey water in a culture dish with the diameter of 3cm for supplementing nutrition to the bombyx batrachyces adult;
s5, after 72h, removing the Brachypodium semiaquilegii, and continuously parasitizing the mealybugs in other insect cages by the Brachypodium semiaquilegii until the mealybugs die;
s6, continuously feeding parasitized Helicoverpa virens by using germinated potatoes, picking up the parasitized Helicoverpa virens after parasitizing for 8 days, putting the parasitized Helicoverpa virens in a 2ml centrifugal tube, waiting for emergence of the parasitized Helicoverpa virens, observing every day at 8:00 in the morning and 20:00 in the evening, and recording the number, emergence time and male and female of the parasitized bees; the emerged parasitic wasps are used for research, field release or propagation of offspring;
and S7, repeating the steps S1-S6, and subculturing the small bane.
Preferably, in step S1, the potatoes are buried in wet sand, and water is sprayed to the sand every 2d to keep the potatoes wet.
Preferably, in step S2, 25 female adult hibiscus solenopsis bugs or third instar nymphs are transferred to a sprouted potato, keeping the sprouted potato with the appropriate number of hibiscus solenopsis bugs. The transferred Chinese hibiscus mealybugs have uniform body size, and the size uniformity of the imagoes of the small bombus batoni is ensured.
Preferably, in step S3, two germinated potatoes are grouped together, and 50 of the Chinese hibiscus pink geckos are placed in an insect-raising box with the specification of 22cm × 15cm × 8cm, and the top of the insect-raising box is sealed by a 100-mesh gauze.
Preferably, in step S3, the insect room is provided with insect-raising shelves having a size of 110cm × 50cm × 200cm, each insect-raising shelf has 4 layers, each layer has a height of 50cm, and the top of each layer of shelf is provided with a lamp tube as a light source. The temperature of the insect room is controlled by an air conditioner, the relative humidity is controlled by a humidifier, the illumination time is controlled by an intelligent switch, and a hygrothermograph is placed for real-time monitoring.
Preferentially, in step S6, the parasitized lycopersicon fuscipes are placed in centrifugal tubes separately, the emergence is observed conveniently, the emergence time range is easy to determine, and the test bees are ensured to be not mated after emergence. The number of emergence parasitic wasps, emergence time, and males and females were recorded each day at 8:00 am and 20:00 pm. The emerged bombus spp is used for further breeding, scientific research or field release.
The invention has the beneficial effects that: the method uses the germinated potatoes to breed the small bangesbeiya, and can shorten the preparation time of the early-stage material so as to avoid the shortage of host plant materials in the test process. A lot of space is saved, i.e. a lot of parasitic bees are available in the same space. And the stiff cockroaches are easy to pick up, so that errors caused by untimely picking up of the insects after the emergence of the Brazilian jumping bees are avoided. Is particularly suitable for research or field release of a large amount of parasitic wasps to quickly control the Leptospermum scoparium. In the invention, the potatoes are easy to germinate, and can be directly purchased in the market, and the germinated potatoes are rotten for about 2 months generally. And the time from parasitism to emergence of the small bombesin is about 15 d. One sprouted potato may be used 3 times. The potatoes are small in size, the required space is small, more insect raising boxes can be placed in the same space, and more Brazilian jumping bees can be raised. The parasitized bearded mealybugs are all on the surface of the potatoes, so that the parasitized bearded mealybugs can be conveniently picked out. The indoor subculture method for the small bans is simple to operate, greatly improves the insect breeding efficiency, and can obtain a large amount of parasitic wasps in a short time for research or field release.
Detailed Description
The following description of specific embodiments of the present invention is provided in connection with examples to facilitate a better understanding of the present invention.
Example 1
In the indoor rearing method of the bombus in the embodiment, the host plant is the ground bean sprouts, and the specific steps are as follows:
s1, burying potatoes weighing about 100g in wet sand, wherein the distance between the tops of the potatoes and the surface of the sand layer is about 2cm, and taking out the potatoes with buds from the sand for later use when the potatoes germinate and the buds grow to about 2 cm;
s2, transferring 25 female imagoes or third instar nymphs of the Chinese hibiscus mealybugs to the germinated potatoes;
s3, placing the germinated potatoes and female old Chinese black mealybugs or third instar nymphs into an insect box with the specification of 22cm multiplied by 15cm multiplied by 8cm, placing the box on insect support frames in an insect room for feeding, wherein the specification of the insect support frames is 110cm multiplied by 50cm multiplied by 200cm, and each insect support frame is divided into four layers, so that 48 insect boxes can be placed in one insect support frame in the insect room, and the feeding conditions are as follows: temperature 28 ± 2 ℃, photoperiod 14L: 10D, relative humidity 80 +/-5%;
s4, placing 4 female bombyx batrachii and 2 male bombyx mori which are not mated after eclosion into a pest breeding box, enabling the bombyx batrachyces to parasitize the bombyx batrachyces for 72 hours, and placing absorbent cotton dipped with 10% of honey water in a culture dish with the diameter of 3cm for supplementing nutrition to the bombyx batrachyces adult;
and (S5) after 72h, removing the Brachypodium shikimchii, and continuously parasitizing the mealybugs in other insect cages until the Brachypodium shikimi dies.
S6, continuously feeding parasitized Helicoverpa virens by using germinated potatoes, picking up the parasitized Helicoverpa virens after 8 days of parasitization, putting the parasitized Helicoverpa virens in a 2ml centrifugal tube, waiting for emergence, observing the parasitized Helicoverpa virens at 8:00 morning and 20:00 night every day, and recording the number, emergence time and male and female sex of the parasitized bees. The emerged parasitic wasps are used for research, field release or propagation of offspring;
and S7, repeating the steps S1-S6, and subculturing the small bane.
Comparative example 1
In the indoor feeding method of the small bans jumping bees in the comparative example, the host plant is the potato seedling, and the specific steps are as follows:
s1, burying potatoes weighing about 100g in wet sand, wherein the distance between the tops of the potatoes and the surface of the sand layer is about 2cm, and waiting for the potatoes to germinate;
s2, when the buds grow to be about 2cm, taking out the potatoes from the sand, planting the potatoes in a flowerpot with the length of 10cm multiplied by 10cm, and watering regularly when the potato seedlings grow to be 25cm for later use so as to prevent the potato seedlings from withering and dying;
s3, transferring 50 female imagoes or third instar nymphs of the hibiscus sinensis mealybugs to potato seedlings with the height of 25 cm;
s4, potato seedlings are placed in insect raising cages for raising, the specification of the insect raising cages is 20cm multiplied by 30cm yarn cages with sleeves, watering is carried out from the cuffs periodically, the insect raising cages are placed on insect raising racks of an insect raising room for raising, the specification of the insect raising racks is 110cm multiplied by 50cm multiplied by 200cm, each insect raising rack is divided into four layers, therefore, 20 insect raising cages can be placed on one insect raising rack in the insect raising room, and the raising conditions are as follows: temperature 28 ± 2 ℃, photoperiod 14L: 10D, relative humidity 80 +/-5%;
s5, placing 4 female bombyx batrachii bees and 2 male bombyx batrachta bees which are not mated after eclosion into an insect breeding cage, enabling the bombyx batrachyces parasitizing to bear the coccico solfatigus for 72 hours, and placing absorbent cotton dipped with 10% of honey water in a culture dish with the diameter of 3cm for supplementing nutrition to the bombyx batrachicola adults;
and (S6) after 72h, removing the Brachypodium shikimchii, and continuously parasitizing the mealybugs in other insect cages until the Brachypodium shikimi dies.
S7, continuously breeding parasitized Helicoverpa virens by using potato seedlings, picking up the parasitized Helicoverpa virens after 8 days of parasitization, putting the parasitized Helicoverpa virens in a 2ml centrifugal tube, waiting for emergence, observing the parasitized Helicoverpa virens at 8:00 morning and 20:00 night every day, and recording the number, emergence time and male and female sex of the parasitized bees. The emerged parasitic wasps are used for research, field release or propagation of offspring;
and S8, repeating the steps S1-S7, and subculturing the small bane.
Comparative example 2
In the indoor feeding method of the Brazilian jumping bee in the comparative example, the host plant is a tomato seedling, and the method comprises the following specific steps:
s1, scattering tomato seeds in a seedling tray for seedling;
s2, transplanting tomato seedlings with the height of 10cm into flowerpots with the height of 10cm multiplied by 10cm, and watering regularly when the tomato seedlings grow to 25cm for later use;
s3, transferring 50 female adults or third instar nymphs of the Hibiscus solenopsis Lecanis to tomato seedlings with the height of 25 cm;
s4, tomato seedlings are placed into insect raising cages for raising, the specification of each insect raising cage is a yarn cage with sleeves, the yarn cage is 20cm multiplied by 30cm, watering is performed regularly from cuffs, the insect raising cages are placed on insect raising frames of an insect raising room for raising, the specification of each insect raising frame is 110cm multiplied by 50cm multiplied by 200cm, each insect raising frame is divided into four layers, therefore, 20 insect raising cages can be placed on one insect raising frame in the insect raising room, and the raising conditions are as follows: temperature 28 ± 2 ℃, photoperiod 14L: 10D, relative humidity 80 +/-5%;
s5, placing 4 female bombyx batrachii bees and 2 male bombyx batrachta bees which are not mated after eclosion into an insect breeding cage, enabling the bombyx batrachyces parasitizing to bear the coccico solfatigus for 72 hours, and placing absorbent cotton dipped with 10% of honey water in a culture dish with the diameter of 3cm for supplementing nutrition to the bombyx batrachicola adults;
and (S6) after 72h, removing the Brachypodium shikimchii, and continuously parasitizing the mealybugs in other insect cages until the Brachypodium shikimi dies.
S7, continuously breeding parasitized Lecanicillium solenopsis with tomato seedlings, picking up the parasitized Lecanicillium solenopsis after parasitizing for 8 days, putting the parasitized Lecanicillium solenopsis in a 2ml centrifuge tube, waiting for emergence, observing each time at 8:00 morning and 20:00 evening every day, and recording the number, emergence time and male and female of the emergence parasitic wasps. The emerged parasitic wasps are used for research, field release or propagation of offspring;
and S8, repeating the steps S1-S7, and subculturing the small bane.
Comparative example 3
In the indoor feeding method of the Brazilian jumping bee in the comparative example, the host plant is cotton seedling, and the specific steps are as follows:
s1, sowing cotton seeds in a flowerpot with the length of 10cm multiplied by 10cm, and watering regularly when cotton seedlings grow to 25cm for later use;
s2, transferring 50 female imagoes or third instar nymphs of the hibiscus sinensis mealybugs to cotton seedlings with the height of 25 cm;
s3, placing cotton seedlings into insect raising cages for raising, wherein the specification of each insect raising cage is a yarn cage with sleeves, the yarn cage is 20cm multiplied by 30cm, watering is performed regularly from cuffs, the insect raising cages are placed on insect raising frames of an insect raising room for raising, the specification of each insect raising frame is 110cm multiplied by 50cm multiplied by 200cm, each insect raising frame is divided into four layers, therefore, 20 insect raising cages can be placed on one insect raising frame in the insect raising room, and the raising conditions are as follows: temperature 28 ± 2 ℃, photoperiod 14L: 10D, relative humidity 80 +/-5%;
s4, placing 4 female bombyx batrachii bees and 2 male bombyx batrachta bees which are not mated after eclosion into an insect breeding cage, enabling the bombyx batrachyces parasitizing to bear the coccico solfatigus for 72 hours, and placing absorbent cotton dipped with 10% of honey water in a culture dish with the diameter of 3cm for supplementing nutrition to the bombyx batrachicola adults;
and (S5) after 72h, removing the Brachypodium shikimchii, and continuously parasitizing the mealybugs in other insect cages until the Brachypodium shikimi dies.
S6, continuously breeding parasitized Lecanicillium solenopsis with cotton seedlings, picking up the parasitized Lecanicillium solenopsis after parasitizing for 8 days, putting the parasitized Lecanicillium solenopsis in a 2ml centrifuge tube, waiting for emergence, observing each time at 8:00 morning and 20:00 evening every day, and recording the number, emergence time and male and female of the emergence parasitic wasps. The emerged parasitic wasps are used for research, field release or propagation of offspring;
and S7, repeating the steps S1-S7, and subculturing the small bane.
Comparative example 4
In the indoor feeding method of the Brazilian jumping wasps in the comparative example, the host plant is Hibiscus rosa-sinensis, and the specific steps are as follows:
s1, purchasing Artocarpus heterophyllus seedlings in the market, and reserving when the Artocarpus heterophyllus seedlings grow to 25cm high;
s2, transferring 50 female adults or third instar nymphs of the Chinese red solenopsis gecko to Chinese red holtzia which is 25cm high;
s3, putting hibiscus into an insect raising cage for raising, wherein the specification of the insect raising cage is a yarn cage with sleeves, the yarn cage is 20cm multiplied by 30cm, watering is carried out from cuffs periodically, the insect raising cage is placed on an insect raising frame of an insect raising room for raising, the specification of the insect raising frame is 110cm multiplied by 50cm multiplied by 200cm, each insect raising frame is divided into four layers, therefore, 20 insect raising cages can be placed on one insect raising frame in the insect raising room, and the raising conditions are as follows: temperature 28 ± 2 ℃, photoperiod 14L: 10D, relative humidity 80 +/-5%;
s4, placing 4 female bombyx batrachii bees and 2 male bombyx batrachta bees which are not mated after eclosion into an insect breeding cage, enabling the bombyx batrachyces parasitizing to bear the coccico solfatigus for 72 hours, and placing absorbent cotton dipped with 10% of honey water in a culture dish with the diameter of 3cm for supplementing nutrition to the bombyx batrachicola adults;
and (S5) after 72h, removing the Brachypodium shikimchii, and continuously parasitizing the mealybugs in other insect cages until the Brachypodium shikimi dies.
S6, continuously breeding parasitized Hibiscus solenopsis with Hibiscus sinensis, picking up the parasitized Hibiscus solenopsis after 8 days of parasitization, placing the parasitized Hibiscus solenopsis in a 2ml centrifuge tube, waiting for emergence, observing each time at 8:00 morning and 20:00 evening every day, and recording the number, emergence time and male and female of the emergence parasitic wasps. The emerged parasitic wasps are used for research, field release or propagation of offspring;
and S7, repeating the steps S1-S6, and subculturing the small bane.
TABLE 1 comparison of results for example 1 and comparative examples 1-4
| Example 1 | Comparative example 1 | Comparative example 2 | Comparative example 3 | Comparative example 4 | |
| Required time before inoculation (d) | 10 | 25 | 60 | 25 | 15 |
| Watering during the period | Whether or not | Is that | Is that | Is that | Is that |
| Specification of insect-raising cage (cm) | 22×15×8 | 20×20×30 | 20×20×30 | 20×20×30 | 20×20×30 |
| Number of insect cages put in the insect breeding rack | 48 | 20 | 20 | 20 | 20 |
| Easy to receive mealybugs | Easy to use | Difficulty in | Difficulty in | Is easier to be | Is difficult to |
| Difficulty and easiness in picking stiff coccid | Easy to use | Difficulty in | Is difficult to | Difficulty in | Is difficult to |
| Parasitic ratio | 81.96％ | 76.94％ | 60.43％ | 72.24％ | 70.43％ |
| Male to female ratio | 1.22：1 | 1.15:1 | 1.62:1 | 1.33：1 | 1.28：1 |
As seen from table 1:
the insect inoculation experiment can be carried out after the potatoes grow to buds about 2cm for 10 days, the seedlings from the seeds to the height of about 25cm for 60 days, the seedlings from the cotton for 25 days, the seedlings from the potatoes for 25 days and the seedlings from the potatoes for 15 days. Therefore, the preparation time of the sprouted potatoes is shortest, and a large number of potatoes can be prepared for standby at one time. Or directly purchasing germinated potato for breeding the Brachypodium banseri.
In all hosts, the other four hosts were watered regularly, except for the sprouted potatoes which did not require watering. In the watering process, the small bans jumping bees are easy to escape, and the efficiency of raising the insects is reduced.
The minimum insect cage size required for the germinated potatoes is found on all hosts, so that 48 insect cages are placed in the germinated potatoes in one shelf of the insect room, and 20 insect cages are placed in the rest. Therefore, the germinated potatoes can obtain more parasitic wasp individuals in the same space, and the insect breeding efficiency is improved.
The method has the advantages that the insect inoculation is difficult and easy, the mealy scale is directly placed on the surface of the potato, the mealy scale is easy to slide and is easy to replace, the smooth degree of the surface of the leaf and the surface area of the leaf need to be considered when the insect is inoculated on the seedling, and the insects need to be placed on the surface of the leaf one by one. And the mealybugs are not easy to be put on the surface again after sliding from the blades, thereby reducing the efficiency.
The selection of the stiff geckos is difficult, the parasitic stiff geckos are selected after the Chinese hibiscus mealybugs are parasitized for 8 days, and the selected stiff geckos are placed in 2ml centrifuge tubes individually to wait for eclosion. Avoiding untimely picking after eclosion from influencing subsequent experimental results. Because the stiff scale is on the surface of the germinated potato, and the stiff scale is concentrated, the stiff scale is easy to pick out. The positions of the stiff cocci on the other four host plants are as follows: the surfaces of the blades, the back surfaces of the blades and the stalks are dispersed and are not easy to pick, and the blades are easy to break in the picking process.
The field release has certain difference due to different host plants, the whole sprouted potato can be put in the field to wait for emergence of parasitic wasps, and the other four host plants can only pick up leaves and put in the field to wait for emergence of parasitic wasps. The blades are light, so that the leaves are easily influenced by weather such as wind and rain in the field, and the leaves in vitro are not easy to store.
The host is different, and the parasitic rate under the same condition is also different. Wherein the parasitic rate on the germinated potatoes is the highest and is 81.96 percent. The tomato seedling has the lowest parasitism rate of 60.43%.
The hosts are different, and the male-female ratio of the generated offspring is different. Experiments show that the male-female ratio of offspring produced by several hosts is not large. Are all about 1.2: 1.
Through the comparison, the germinated potatoes are adopted for breeding the Brazilian jumping bees, so that a large amount of time can be saved before inoculation. And after the insects are inoculated, the same amount of the insects is inoculated, so that a large amount of space can be saved. The potatoes are easy to germinate, are easy to store after germination, are not easily influenced by weather, and avoid the influence of the weather on the growth speed of the plants. Therefore, the germinated potatoes are used for the subculture propagation of the Brazilian jumping bees, and the method has the advantages of simple operation and capability of obtaining a large number of standard parasitic wasps in a short time.
While the foregoing is directed to the preferred embodiment of the present invention, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention.
Claims (1)
1. An indoor subculture method of Brazilian jumping bees is characterized by comprising the following steps: the method comprises the following steps:
s1, burying 100g potatoes into wet sand, enabling the tops of the potatoes to be 2cm away from the surface of a sand layer, waiting for the potatoes to sprout until the sprouts grow to 2cm, and taking out the potatoes with the sprouts from the sand for later use;
s2, transferring 25 female imagoes or third instar nymphs of the Chinese hibiscus mealybugs to the germinated potatoes;
s3, placing the germinated potatoes and female adult and third-instar nymphs of the mealybugs of the hibiscus sinensis into an insect box with the specification of 22cm multiplied by 15cm multiplied by 8cm, and placing the box on an insect support in an insect room for feeding, wherein the feeding conditions are as follows: temperature 28 ± 2 ℃, photoperiod 14L: 10D, relative humidity 80 +/-5%;
s4, placing 4 female bombyx batrachii and 2 male bombyx mori which are not mated after eclosion into a pest breeding box, enabling the bombyx batrachyces to parasitize the bombyx batrachyces for 72 hours, and placing absorbent cotton dipped with 10% of honey water in a culture dish with the diameter of 3cm for supplementing nutrition to the bombyx batrachyces adult;
s5, after 72h, removing the Brachypodium semiaquilegii, and continuously parasitizing the mealybugs in other insect cages by the Brachypodium semiaquilegii until the mealybugs die;
s6, continuously feeding parasitized Helicoverpa virens by using germinated potatoes, picking up the parasitized Helicoverpa virens after parasitizing for 8 days, putting the parasitized Helicoverpa virens in a 2ml centrifugal tube, waiting for emergence of the parasitized Helicoverpa virens, observing every day at 8:00 in the morning and 20:00 in the evening, and recording the number, emergence time and male and female of the parasitized bees; the emerged parasitic wasps are used for research, field release or propagation of offspring;
s7, repeating the steps S1-S6, and subculturing the Bay small jumping bees;
in step S1, the potatoes are buried in wet sand, and water is sprayed to the sand every 2d to keep the potatoes wet;
in the step S2, 25 female adults or three-instar nymphs of the mountain pickup mealybugs are transferred to one sprouted potato; the transferred Chinese hibiscus mealybugs have uniform body size, and the size uniformity of the imagoes of the small bombus batoni is ensured;
in the step S3, two germinated potatoes are combined into a group, 50 hibiscus mealybugs are placed in an insect box with the specification of 22cm multiplied by 15cm multiplied by 8cm, and the top of the insect box is sealed by a gauze with 100 meshes; in the step S3, insect culturing frames are placed in the insect culturing room, the specification of the insect culturing frames is 110cm multiplied by 50cm multiplied by 200cm, each insect culturing frame has 4 layers, each layer is 50cm high, and the top of each layer of the frame is provided with a lamp tube as a light source; the temperature of the insect room is controlled by an air conditioner, the relative humidity is controlled by a humidifier, the illumination duration is controlled by an intelligent switch, and a hygrothermograph is arranged for real-time monitoring;
in the step S6, the parasitized Lecanicillium solenopsis is independently placed in a centrifuge tube, the eclosion of the parasitized Lecanicillium solonii is conveniently observed, the range of the eclosion time is easily determined, and the test adopted Begonia banseri is ensured to be not mated after the eclosion; observing each day at 8:00 morning and 20:00 evening, and recording the number, emergence time and male and female of emergence parasitic wasps; the emerged bombus spp is used for further breeding, scientific research or field release.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910791379.5A CN110521674B (en) | 2019-08-26 | 2019-08-26 | Indoor breeding method for Brachypodium banseri |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910791379.5A CN110521674B (en) | 2019-08-26 | 2019-08-26 | Indoor breeding method for Brachypodium banseri |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN110521674A CN110521674A (en) | 2019-12-03 |
| CN110521674B true CN110521674B (en) | 2021-12-24 |
Family
ID=68664302
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910791379.5A Active CN110521674B (en) | 2019-08-26 | 2019-08-26 | Indoor breeding method for Brachypodium banseri |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110521674B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112753656B (en) * | 2020-12-31 | 2023-03-17 | 广西壮族自治区农业科学院 | Breeding device and indoor seed preservation method for Hemerocallis ventricosus such as natural enemy of sugarcane striped rice borer |
| CN113439715A (en) * | 2021-07-30 | 2021-09-28 | 浙江省农业科学院 | Indoor successive transfer breeding method of lycoris radiata mealybugs |
| CN113678790B (en) * | 2021-07-30 | 2023-02-24 | 浙江省农业科学院 | Method for optimizing Babylonia wasp population structure in host conversion mode |
| CN113951214B (en) * | 2021-12-01 | 2023-05-05 | 浙江省农业科学院 | Method for prolonging storage time of ban jumping bees and application |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102742555A (en) * | 2012-07-07 | 2012-10-24 | 山西出入境检验检疫局检验检疫技术中心 | Method for raising phenacoccus solenopsis tinsley |
| CN104094902B (en) * | 2014-07-21 | 2016-03-16 | 广西壮族自治区农业科学院植物保护研究所 | The indoor continuously rearing method of the continuous mealybug of a kind of Japan |
| CN107980729B (en) * | 2017-12-07 | 2020-03-06 | 浙江省萧山棉麻研究所 | Method for indoor breeding mealybugs and Brachypodium bambusicola |
-
2019
- 2019-08-26 CN CN201910791379.5A patent/CN110521674B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN110521674A (en) | 2019-12-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110521674B (en) | Indoor breeding method for Brachypodium banseri | |
| CN103385220B (en) | Scale breeding method for encarsia formosa | |
| KR100797933B1 (en) | Seedling-raising method in strawberry | |
| CN104082009B (en) | The method of a kind of willow greenhouse seeding and seedling raising | |
| CN105191763A (en) | Vegetable organic soil-less culture method, culture medium and manufacturing method thereof | |
| CN102893799A (en) | Automated vegetable planting and harvesting production line | |
| KR101866682B1 (en) | Early flower initiation induction of strawberry transplants by using functional tray inserted with paper pot | |
| CN106561575B (en) | Artificial feeding method for diaphorina citri | |
| CN110506620A (en) | A kind of greenhouse vegetable rock wool recycling method based on crop rotation | |
| KR101589414B1 (en) | Strawberry growth method using growth container | |
| CN113519468A (en) | Method for feeding phyllotreta striolata in indoor generation | |
| CN113558014B (en) | Large-scale production method of dormitory lice flea bees | |
| CN103210881A (en) | Large-scale artificial breeding method for Neoseiulu californicus | |
| CN105409556A (en) | Planting method for strawberries | |
| WO2019048382A1 (en) | Method for production of sugarcane seedlings | |
| KR101281934B1 (en) | Nado Lantern New breed Prince | |
| CN111758328B (en) | Microenvironment breeding method for thinly flowered cypress branches | |
| CN105475232B (en) | Method for large-scale feeding and aphid control of aphidiidae | |
| CN114568307A (en) | Method for rapidly breeding seedlings by utilizing alpinia katsumadai stem tips | |
| CN114568064A (en) | Pineapple seed breathable water-retention breeding method | |
| CN103125329A (en) | Cultivation method of indoor miniature landscape peach tree | |
| CN111543396A (en) | Indoor large-scale breeding method for small flies and pupa terracotta warriors | |
| CN111742729A (en) | Method for rapid propagation of high-quality tomato seedlings | |
| KR20100119081A (en) | Mass production method of virus-free potato seeds | |
| CN110959581A (en) | Artificial propagation method for psylla chinensis parasitic wasps and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |