CN104094902B - The indoor continuously rearing method of the continuous mealybug of a kind of Japan - Google Patents
The indoor continuously rearing method of the continuous mealybug of a kind of Japan Download PDFInfo
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- CN104094902B CN104094902B CN201410347461.6A CN201410347461A CN104094902B CN 104094902 B CN104094902 B CN 104094902B CN 201410347461 A CN201410347461 A CN 201410347461A CN 104094902 B CN104094902 B CN 104094902B
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Abstract
The invention discloses the indoor continuously rearing method of the continuous mealybug of a kind of Japan, comprise the following steps: S1, the outstanding bell flower of cultivation are as host plant; S2, continuous for Japan mealybug is transferred to outstanding bell takes; S3, in insectary, the continuous mealybug of Japan to be raised; S4, repetition step S1 ~ S3, carry out continuously rearing to the continuous mealybug of Japan.Outstanding bell flower is Japan continuous mealybug eating host, and evergreen all the year round, and plant survival rate is high, and all right cottage propagation, reduces feeding cost.Outstanding bell flower bearing capacity is large, is beneficial to the continuous mealybug breeding of Japan.The indoor continuously rearing method step of the continuous mealybug of Japan is simple, is easy to operation.
Description
Technical field
The present invention relates to a kind of coccid raising method, particularly the indoor continuously rearing method of the continuous mealybug of a kind of Japan.
Background technology
The continuous mealybug (PhenacoccussolenopsisTinsley) of Japan, belong to Homoptera, Pseudococcidae, continuous Eriococcinae, Phenacoccus, comes from North America, is the pernicious invasion insect of extensively distribution in a kind of world wide.In the whole world, 38 countries and regions are distributed to; In China, comprise 14 provinces and cities of Taiwan, all have its trace.
The continuous mealybug host range of Japan is wide, and 57 sections 149 belong to 207 Plants and are its host, contain the plants such as crops, ornamental plants, vegetables, shrub, fruit tree and weeds.Phloem or mesophyll absorption nutrition is inserted with sucking mouth parts, and inject noxious material, cause that host is sallow, depauperation, torsional deformation, even dead, the honeydew of excretion attracts ant, and cause the generation of dark mildew, reduce photosynthesis of plant, occur time serious burning symptom, reduce plant product as the salability of fruit etc.; Except direct and consequential damages crops, it is as quarantine object, adds the cost preventing or eliminate them and produce, causes huge economic loss.In India and Pakistan, cotton field is endangered because of it, loses up to more than 40%, and some cotton field even has no harvest, and causes calamitous strike, bring tremendous economic to lose to cotton grower to Cotton Industry.Propagating to stop it, reducing its harm, the Ministry of Agriculture and State Administration for Quality Supervision and Inspection and Quarantine are classified as quarantine object.
Risk analysis result shows, China's three cotton regions is the height normal region of this insect in interior most area, and continuous mealybug next one harm country of analyses and prediction Japan of International Cotton Advisory Committee will be Chinese, shows that it spreads in China having a high potential of spreading.
For this insect China many distribute, and some flowers and economic crops are produced to the reality of harm, and expand the expection of harm, biological property is studied, to predict its occurrence tendency; Its prophylactico-therapeutic measures is studied, spreads to control its diffusion, to be necessary very much and very urgent.And indoors artificial continuously rearing, obtaining a large amount of examination worms, is the prerequisite of carrying out every research.The raising method of the continuous mealybug of Chinese patent 201210233516.1 1 kinds of Japan, disclosing with potato is the method that the continuous mealybug of Japan raised by foodstuff.But the bearing capacity of potato to the continuous mealybug of Japan is limited, and potato seedling is easily dead in feeding process, be unfavorable for the continuous mealybug breeding of Japan, and potato cost is higher.
Summary of the invention
The object of this invention is to provide the indoor continuously rearing method of the continuous mealybug of Japan that a kind of cost is low, step is simple, Japan's continuous mealybug amount to obtain is large.
For achieving the above object, according to an aspect of the present invention, provide the indoor continuously rearing method of the continuous mealybug of a kind of Japan, comprise the following steps:
S1, the outstanding bell flower of cultivation are as host plant;
S2, continuous for Japan mealybug is transferred to outstanding bell takes;
S3, in insectary, the continuous mealybug of Japan to be raised;
S4, repetition step S1 ~ S3, carry out continuously rearing to the continuous mealybug of Japan.
Thus, outstanding bell is spent evergreen all the year round, and all right cottage propagation, reduces feeding cost.Outstanding bell flower bearing capacity is large, is beneficial to the continuous mealybug breeding of Japan.The indoor continuously rearing method step of the continuous mealybug of above-mentioned Japan is simple, is easy to operation.
In some embodiments, in step sl, outstanding bell is spent during high 18 ~ 20cm and is pinched, when high 45 ~ 50cm spent by outstanding bell as host plant for raising the continuous mealybug of Japan.Thus, pinching and can promote outstanding bell flower collateral generation, for raising the continuous mealybug of Japan when outstanding bell flower reach a certain height, the survival of outstanding bell flower and best bearing capacity can be guaranteed.
In some embodiments, in step s 2, the continuous mealybug of Japan is female adult pest.Thus, female adult pest is laid eggs at outstanding bell flower, realizes the breeding of the continuous mealybug of Japan.
In some embodiments, in step s 2, every strain is hanged bell and is taken the continuous mealybugs of transfer 10 Japan, thus makes every strain hang the appropriate bearing capacity of bell flower tool.
In some embodiments, in step s3, the rearing conditions of the continuous mealybug adult of Japan is: temperature 29 ~ 31 DEG C, photoperiod 14L:10D, intensity of illumination 10000LX, relative moisture 40 ~ 60%, the rearing conditions of the continuous mealybug nymph of Japan is: temperature 25 ~ 27 DEG C, photoperiod 14L:10D, intensity of illumination 10000LX, relative moisture 60 ~ 80%.Thus, above-mentioned rearing conditions can make the continuous mealybug of Japan have shorter development duration, shorter population doubling time, more offspring.
In some embodiments, in step s3, the development duration of the continuous mealybug of Japan is 20 ~ 23 days, and every two days of period spent a supplementary moisture to outstanding bell, and once, fertilizing amount is every strain 1.6 ~ 2.4g in every two weeks fertilising.Thus, keep the skin wet and apply fertilizer between feeding period outstanding bell flower, the growth of outstanding bell flower can be ensured.
In some embodiments, in step s3, arrange foster worm frame in insectary, supporting worm frame is multilayer, and outstanding bell flower is placed on foster worm frame.Thus, outstanding bell flower multilayer is placed, and saves space, and then reduces costs.
Many group experiments can verify that the suitable rearing conditions of the continuous mealybug of Japan and the suitableeest host plant are outstanding bell flower below.
Test the checking of the continuous mealybug rearing conditions of 1 Japan
The continuous mealybug of Japan is raised and is carried out in climatic cabinate, 15,20,25,30 and 35 DEG C of 5 thermogrades are set and 45%, 60%, 75%3 relative moisture carry out dual factors Orthogonal Composite, establish 15 groups of process altogether, the photoperiod is 14L: 10D, intensity of illumination 10000LX.
In Japan's continuous mealybug farrowing Sheng phase, nymph (hatch time≤24h) 40 is incubated with in the same size, in good health first of the writing brush picking of softness, be inoculated in stalwartness, without on the Chinese Hibiscu seedling of pesticide pollution, put into climatic cabinate to raise, every strain Chinese Hibiscu seedling is repeat for 1 time, often organizes rearing conditions and arranges 5 strain Chinese Hibiscu seedling.The quantity in each worm age is observed, is recorded in timing every day, and keep the skin wet to Chinese Hibiscu in time, once, every strain is about 1.0g to every circumferential Chinese Hibiscu sealing fertilizer.Grow to adult to all examination worms, add up female, male worm quantity.
The female worm of the continuous mealybug of Japan by vegetative stage be divided into one age nymph, two age nymph, tritonymph and adult.One age nymph, long 0.71 ~ 0.73mm, wide 0.36 ~ 0.38mm.Two age nymph, long 0.75 ~ 1.10mm, wide 0.36 ~ 0.65mm.Tritonymph, long 1.02 ~ 1.73mm, wide 0.82 ~ 1.00mm.Adult, long 3.00 ~ 4.20mm, wide 2.00 ~ 3.10mm.
With the aforementioned healthy and strong female adult pest of the writing brush picking of softness (female adult pest worm age≤24h) 10, be inoculated in stalwartness, without on the Chinese Hibiscu seedling of pesticide pollution, put into above-mentioned corresponding climatic cabinate and raise, every strain is repeat for 1 time, often processes 5 times and repeats.Every day timing observe, record one age nymph quantity after removed, keep the skin wet in time, sealing fertilizer is once weekly, and every strain is about 1.0g, until all female adult pest are dead.Composite fertilizer, total nitrogen content >=15%, available phosphorus content >=15%, effective potassium content >=15%, granularity 90%.
Table 1 is the survival rate of the continuous mealybug of Japan under different temp. and humidity.Can draw, one age nymph the most responsive to variations in temperature, answer special concern during raising.Temperature is too high or too lowly all cause age nymph mortality.20 ~ 30 DEG C, whole nymph survival rate is mostly more than 50.00%, and in this temperature range, nymph can survive mostly, and 25 DEG C and the combination of 75% relative moisture, nymph survival rate is up to 84.00%.
Table 2 is the development duration of the continuous mealybug of Japan under different temp. and humidity.Can draw, if one, two, three ages to go through the phase close, each worm age and whole nymph development duration raise with temperature and fall after rising, and 25 ~ 30 DEG C of development durations are shorter, and 30 DEG C and 45% relative moisture combine, and nymph is the shortest, is only 12.30 days.
Table 3 is the female worm percentage of the continuous mealybug of Japan, life-span and fertility under different temp. and humidity.Can draw, too high or too low for temperature, female worm percentage is all lower, 25 DEG C and the combination of 75% relative moisture, and female worm percentage is the highest, is 90.00%; 20 DEG C of female adult pest longest-liveds; 15,35 DEG C, female worm can not farrow, 25 DEG C and the combination of 60% relative moisture, and 30 DEG C and the combination of 45% relative moisture, and female worm farrowing amount is all more than 400.00.
Table 4 is the life table parameters of the continuous mealybug of Japan under different temp. and humidity.Can draw, 30 DEG C and the combination of 45% relative moisture, Japan's average generation time of continuous mealybug is only 21.81 days, and the population doubling time is 3.47 days, compares to be beneficial to the continuous mealybug of Japan and to grow.
In sum, the suitable temp. and humidity in different worm age is different, and 25 DEG C and the combination of 75% relative moisture, compare and be beneficial to nymph growth, and the female worm more than obtaining relatively; 30 DEG C and the combination of 45% relative moisture, Japan's female worm fertility of continuous mealybug is strong, can obtain more offspring.Therefore, in larval phase, select 25 DEG C and the continuous mealybug of 75% relative moisture combination raising Japan, when the adult stage raises up seed, select 30 DEG C and the continuous mealybug of 45% relative moisture combination raising Japan.
The survival rate of the continuous mealybug of Japan under the different temp. and humidity of table 1
Note: in table, data are mean value ± standard error, with the difference letter after column data, represent through Duncan formula Multiple range test significant difference (P=0.05), following table is same.
The development duration of the continuous mealybug of Japan under the different temp. and humidity of table 2
The female worm percentage of the continuous mealybug of Japan, life-span and fertility under the different temp. and humidity of table 3
The life table parameters of the continuous mealybug of Japan under the different temp. and humidity of table 4
Test the checking of the continuous mealybug host plant for rearing of 2 Japan
The determination of Japan continuous mealybug eating host: get that 10 ~ 15cm height nutrition pot culture tomato, Chinese Hibiscu, beggar-ticks, Ipomoea batatas, potato, outstanding bell are colored, each strain of cotton seedling, form a circle on laboratory table top, the continuous mealybug 200 of Japan is placed by circle central authorities, unrestricted choice host is let alone under normal temperature laboratory, surrounding water segregation is in case the continuous mealybug of Japan is escaped, record the continuous mealybug quantity of the upper Japan of various host after 3 days respectively, every worm age is a process, often processes 5 repetitions.
Table 5 is that the continuous mealybug of Japan is to the selectivity of host.Can draw, outstanding bell flower is continuous mealybug institute of Ge Ling Japan eating.
The continuous mealybug of table 5 Japan is to the preference of host
Note: in table, insect population percentage (%) selects the population number of certain host to account for the percentage of all hosts this worm instar mouth total amount upper for the continuous mealybug of certain Chong Ling Japan.
The continuous mealybug of Japan grows the determination of optimum host:
Be connected on tomato, Chinese Hibiscu, beggar-ticks, Ipomoea batatas, potato, outstanding bell flower, cotton seedling with incubating nymph in an age (hatch time≤24h) at the beginning of the continuous mealybug of in the same size, the healthy and strong Japan of the writing brush picking of softness, be put in climatic cabinate and raise (temperature 30 ± 1 DEG C, relative moisture 70 ± 8%, photoperiod 14L:10D, intensity of illumination 10000LX).It is repeat for 1 time that every strain connects worm 40, and often kind of host repeats for 5 times.The quantity in each worm age is observed, is recorded in timing every day, keeps the skin wet in time to host plant, and give host plant sealing fertilizer once weekly, every strain is about 1.0g.Grow to adult to all examination worms, add up female, male worm quantity.
With the aforementioned healthy and strong female adult pest of the writing brush picking of softness (female adult pest worm age≤24h) 10, be connected on corresponding host seedling, put into climatic cabinate (temperature 30 ± 1 DEG C, relative moisture 70 ± 8%, photoperiod 14L:10D, intensity of illumination 10000LX) middle raising, it is repeat for 1 time that every strain connects worm 10, and often kind of host repeats for 5 times.Every day timing observe, record one age nymph quantity after removed, keep the skin wet in time, sealing fertilizer is once weekly, and every strain is about 1.0g, until all female adult pest are dead.
Table 6 is the survival rate that different host raises the continuous mealybug of Japan.Can draw, after raising the continuous mealybug of Japan with beggar-ticks, Ipomoea batatas and outstanding bell flower, each worm age and whole nymph survival rate all higher.
Table 7 is the development duration that different host raises the continuous mealybug of Japan.Can draw, raise the continuous mealybug of Japan with different host, each worm age to go through the phase close, raise the continuous mealybug of Japan with outstanding bell flower, nymphal development is very fast, whole nymph only 11.67 days.
Table 8 takes food the female worm percentage after different host, life-span and farrowing amount for the continuous mealybug of Japan.Can draw, raise the continuous mealybug of Japan with outstanding bell flower, although female worm percentage is lower, the female adult pest life-span is shorter, and female worm fertility is very strong, up to 647.92/female, be significantly higher than other host.
Table 9 is the life table parameters after the continuous mealybug of Japan takes food different host.Can draw, raise the continuous mealybug of Japan with outstanding bell flower, its average generation time is the shortest, is only 21.17 days, and clean growth rate is 238.14, and population increases by 1 times, only needs 2.68 days.
To sum up show, outstanding bell flower is Japan continuous mealybug eating host, and this host to grow optimum host for the continuous mealybug of Japan.
The different host of table 6 raises the survival rate of the continuous mealybug of Japan
The different host of table 7 raises the development duration of the continuous mealybug of Japan
The continuous mealybug of table 8 Japan takes food the female worm percentage after different host, life-span and farrowing amount
| Host | Female worm percentage (%) | The female adult pest life-span (my god) | Farrowing amount (head) |
| Tomato | 69.08±2.40a | 41.03±1.42c | 199.83±11.18d |
| Chinese Hibiscu | 59.31±1.01b | 57.15±1.77a | 339.41±11.33c |
| Beggar-ticks | 61.25±2.14b | 62.10±2.77a | 195.33±11.65d |
| Ipomoea batatas | 61.00±2.23b | 40.99±1.29c | 171.09±6.24d |
| Potato | 69.00±1.05a | 57.13±2.06a | 503.33±13.12b |
| Outstanding bell flower | 53.55±2.29c | 45.44±1.53bc | 647.92±9.51a |
| Cotton | 62.26±2.50b | 49.07±1.04b | 336.80±12.95c |
The continuous mealybug of table 9 Japan takes food the life table parameters after different host
In sum, beneficial effect of the present invention is: outstanding bell flower is Japan continuous mealybug eating host, and this host to grow optimum host for the continuous mealybug of Japan.Outstanding bell flower, as host plant, easily obtains, after initial purchase, gets final product cottage propagation, reduce feeding cost.Outstanding bell flower is woody plant, evergreen all the year round, the anniversary can provide food for the continuous mealybug of Japan, realize the uninterrupted breeding of the continuous mealybug of Japan, at any time for test provides neat worm source.Support the layering of worm frame to raise, save space, greatly reduce cost.The continuous mealybug rearing method of Japan, step is simply easy to operation, and equipment needed thereby is simple, and cost is low.
Embodiment
The indoor continuously rearing method of the continuous mealybug of Japan of present embodiment comprises the following steps:
The indoor continuously rearing method of the continuous mealybug of Japan, comprises the following steps:
S1, the outstanding bell flower of cultivation are as host plant;
S2, continuous for Japan mealybug is transferred to outstanding bell takes;
S3, in insectary, the continuous mealybug of Japan to be raised;
S4, repetition step S1 ~ S3, carry out continuously rearing to the continuous mealybug of Japan.
The indoor continuously rearing basic facilities of the continuous mealybug of Japan comprise outdoor solarium, indoor insectary and foster worm frame.
The outdoor long 20m in solarium, wide 8m, high 3m.Ground in outdoor solarium is divided into two furrow, the passageway that centre stays 1.5m wide.The top of outdoor solarium is clear glass, and surrounding 80 order stainless steel mesh shroud.Tap is furnished with in outdoor solarium.
The indoor long 6m in insectary, wide 4m, high 3m.The wall of insectary is provided with window, and window screening is 80 order stainless steel gauzes.It is ventilated that 1 exhaust fan is installed in insectary, 1.2 air conditioner control temperature, the microcomputer time-controlled switch controlled light time, and humidifier controls relative moisture.
Foster worm frame in insectary is 10.Support the long 150cm of worm frame, wide 50cm, high 200cm.Each foster worm frame is divided into two-layer up and down, and be highly respectively 1m, every layer of bottom is the clear glass that 0.5cm is thick, and the fluorescent lamp of 5 36W is installed at every layer of top.Fluorescent lamp is connected with microcomputer time-controlled switch, every layer of outfit fluorescent lamp hand switch one.
Below each step is explained in detail.
S1, the outstanding bell flower of cultivation are as host plant.Operate as follows.
Fertilizer needed for the cultivation of outstanding bell flower and plant modifying agent: composite fertilizer, total nitrogen content >=15%, available phosphorus content >=15%, effective potassium content >=15%, granularity 90%; Soil is spent to be general soil of spending; Root-inducing powder; Above material is commercially available, brand and producer free.
Miao Yuan: buy the high nutrition pot culture of 15cm from market and hang bell flower seedling.
Little transplantation of seedlings is spent to obtain transplanted seedling on the outstanding bell buying gained: again to purchase nutritive cube (21cm × 17cm), bottom nutritive cube, spread that 5cm is thick spends soil, the nutritive cube buying seedling is wiped out with scissors, the whole strain of seedling band soil is transplanted in nutritive cube, replenish that 10cm is thick spends soil, remaining 2cm space, puts to outdoor Yang Chong solarium.Fine day, waters once every day, and the cloudy day waters once for every three days.Once, every strain is about 2.0g to every two weeks sealing fertilizer, grows to 18 ~ 20cm and pinches, break apical dominance, promotes collateral generation.Grow to 45 ~ 50cm for the continuous mealybug of indoor feeding Japan.
Cuttage seeding is obtained by cottage propagation: spring after the outstanding bell flower seedling bought grows up to, the old branch 10 ~ 15cm of clip stalwartness, bring to indoor, the root-inducing powder with 0.02 ~ 0.05% soaks branch lower end and is about 30s, then branch lower end is inserted husky bed about the 5cm degree of depth.Husky bed is soft thin river sand, is layered in the plastic basin of long 50cm, wide 30cm, high 15cm, and with the moistening husky bed of the hand-held watering can of LT908, water is too much unsuitable, in order to avoid ponding, branch is downright bad.Be placed in indoor, note observing, timely replenishing water and preserving moisture, namely roots for about 20 days.Then in outdoor solarium, carry out transplanting process by the method for transplanted seedling, grow to 45 ~ 50cm for the continuous mealybug of indoor feeding Japan.
As from the foregoing, outstanding bell flower, as host plant, easily obtains, after initial purchase, gets final product cottage propagation, reduce feeding cost.
S2, continuous for Japan mealybug is transferred to outstanding bell takes.Operate as follows.
The preparation in the continuous mealybug source of Japan: the first tenday period of a month in May, from field acquisition with the Chinese Hibiscu blade of the continuous mealybug female adult pest of Japan or branch, brings to insectary.Said process notes protection, in order to avoid the continuous mealybug of Japan is escaped, caused anthropochory.
45 ~ 50cm the transplanted seedling obtained by step S1 or cuttage seeding are put on the foster worm frame in insectary, hold with plastics holder.The continuous mealybug side of the Japan of touching on Chinese Hibiscu with fine, soft fur pen, makes its lancet extract from Chinese Hibiscu blade or branch, then transfers them on outstanding bell floral leaf sheet with writing brush.Every strain reception farrowing female adult pest 10.
S3, in insectary, the continuous mealybug of Japan to be raised.Operate as follows.
Be transferred to 10 female adult pest that outstanding bell takes when not laying eggs, the condition in insectary is: temperature 29 ~ 31 DEG C, photoperiod 14L:10D, intensity of illumination 10000LX, relative moisture 40 ~ 60%.
After an age, (4 ~ 6 days after egg capsule appearance) appearred in nymph in a large number, the condition of adjustment insectary is: temperature 25 ~ 27 DEG C, photoperiod 14L:10D, intensity of illumination 10000LX, relative moisture 60 ~ 80%.
The development duration of the continuous mealybug of Japan is 20 ~ 23 days, and every two days of period spent a supplementary moisture to outstanding bell, and once, fertilizing amount is every strain 1.6 ~ 2.4g in every two weeks fertilising.
Treat that nymph grows to adult, continue transfer 10 female insects to new outstanding bell and take continuously rearing.Per 20 ~ 23 days generations.Every strain can continuous mealybug one nymph in age about 5500 of output Japan, or two age nymph 5000, or tritonymph 4500, or female adult pest 2000.Can be the continuous mealybug of Japan that indoor scientific research provides uniform in a large number, ensure carrying out smoothly of scientific research.
Above-described is only some embodiments of the present invention.For the person of ordinary skill of the art, without departing from the concept of the premise of the invention, can also make some distortion and improvement, these all belong to protection scope of the present invention.
Claims (5)
1. an indoor continuously rearing method for the continuous mealybug of Japan, is characterized in that, comprise the following steps:
S1, the outstanding bell flower of cultivation are as host plant;
S2, continuous for Japan mealybug is transferred to outstanding bell takes, the continuous mealybug of Japan of transfer produces female adult pest for waiting;
S3, in insectary, the continuous mealybug of Japan to be raised, the rearing conditions of the continuous mealybug adult of Japan is: temperature 29 ~ 31 DEG C, photoperiod 14L:10D, intensity of illumination 10000LX, relative moisture 40 ~ 60%, the rearing conditions of the continuous mealybug nymph of Japan is: temperature 25 ~ 27 DEG C, photoperiod 14L:10D, intensity of illumination 10000LX, relative moisture 60 ~ 80%;
S4, repetition step S1 ~ S3, carry out continuously rearing to the continuous mealybug of Japan.
2. the indoor continuously rearing method of the continuous mealybug of Japan according to claim 1, it is characterized in that, in step sl, outstanding bell is spent during high 18 ~ 20cm and is pinched, when high 45 ~ 50cm spent by outstanding bell as host plant for raising the continuous mealybug of Japan.
3. the indoor continuously rearing method of the continuous mealybug of Japan according to claim 1, is characterized in that, in step s 2, every strain is hanged bell and taken the continuous mealybugs of transfer 10 Japan.
4. the indoor continuously rearing method of the continuous mealybug of Japan according to claim 1, is characterized in that, in step s3, the development duration of the continuous mealybug of described Japan is 20 ~ 23 days, every two days of period spent a supplementary moisture to outstanding bell, and once, fertilizing amount is every strain 1.6 ~ 2.4g in every two weeks fertilising.
5. the indoor continuously rearing method of the continuous mealybug of Japan according to claim 1, it is characterized in that, in step s3, arrange foster worm frame in described insectary, described foster worm frame is multilayer, and described outstanding bell flower is positioned on foster worm frame.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106797919A (en) * | 2017-03-08 | 2017-06-06 | 四川省农业科学院经济作物育种栽培研究所 | A kind of method for breeding of middle-size and small-size insect |
| CN110521674A (en) * | 2019-08-26 | 2019-12-03 | 广西壮族自治区农业科学院植物保护研究所 | A kind of indoor feeding method of Ban Shi encyrtid |
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