CN110959581A - Artificial propagation method for psylla chinensis parasitic wasps and application thereof - Google Patents
Artificial propagation method for psylla chinensis parasitic wasps and application thereof Download PDFInfo
- Publication number
- CN110959581A CN110959581A CN201911200921.1A CN201911200921A CN110959581A CN 110959581 A CN110959581 A CN 110959581A CN 201911200921 A CN201911200921 A CN 201911200921A CN 110959581 A CN110959581 A CN 110959581A
- Authority
- CN
- China
- Prior art keywords
- pear
- chinensis
- psylla
- psylla chinensis
- nymphs
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241001486393 Cacopsylla chinensis Species 0.000 title claims abstract description 69
- 241001481304 Vespoidea Species 0.000 title claims abstract description 22
- 238000000034 method Methods 0.000 title claims abstract description 22
- -1 pplication Species 0.000 title abstract description 4
- 235000014443 Pyrus communis Nutrition 0.000 claims abstract description 61
- 241000238631 Hexapoda Species 0.000 claims abstract description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 21
- 241000257303 Hymenoptera Species 0.000 claims abstract description 19
- 235000012907 honey Nutrition 0.000 claims abstract description 16
- 230000024241 parasitism Effects 0.000 claims abstract description 12
- 230000013011 mating Effects 0.000 claims abstract description 6
- 239000002420 orchard Substances 0.000 claims abstract description 6
- 238000011161 development Methods 0.000 claims abstract description 5
- 241001674048 Phthiraptera Species 0.000 claims abstract description 4
- 241000220324 Pyrus Species 0.000 claims abstract 22
- 241001466030 Psylloidea Species 0.000 claims description 16
- 235000013601 eggs Nutrition 0.000 claims description 12
- 240000001624 Espostoa lanata Species 0.000 claims description 11
- 235000009161 Espostoa lanata Nutrition 0.000 claims description 11
- 229920000742 Cotton Polymers 0.000 claims description 8
- 235000016709 nutrition Nutrition 0.000 claims description 8
- 241000256856 Vespidae Species 0.000 claims description 7
- 238000012258 culturing Methods 0.000 claims description 7
- 235000015097 nutrients Nutrition 0.000 claims description 6
- 230000035764 nutrition Effects 0.000 claims description 6
- 230000018109 developmental process Effects 0.000 claims description 4
- 238000005286 illumination Methods 0.000 claims description 4
- 230000017448 oviposition Effects 0.000 claims description 4
- 238000009423 ventilation Methods 0.000 claims description 4
- 229910052902 vermiculite Inorganic materials 0.000 claims description 4
- 235000019354 vermiculite Nutrition 0.000 claims description 4
- 239000010455 vermiculite Substances 0.000 claims description 4
- 238000009395 breeding Methods 0.000 claims description 3
- 230000035784 germination Effects 0.000 claims description 3
- 239000002689 soil Substances 0.000 claims description 3
- 238000009331 sowing Methods 0.000 claims description 3
- 238000005238 degreasing Methods 0.000 claims description 2
- 239000011159 matrix material Substances 0.000 claims description 2
- 241000294063 Cacopsylla bidens Species 0.000 claims 1
- 230000010196 hermaphroditism Effects 0.000 claims 1
- 230000000384 rearing effect Effects 0.000 abstract description 7
- 244000045947 parasite Species 0.000 abstract 1
- 240000001987 Pyrus communis Species 0.000 description 39
- 230000003071 parasitic effect Effects 0.000 description 19
- 241000526200 Cacopsylla pyri Species 0.000 description 12
- 230000032669 eclosion Effects 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 239000000575 pesticide Substances 0.000 description 5
- 230000003203 everyday effect Effects 0.000 description 4
- 239000004033 plastic Substances 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 241000526145 Psylla Species 0.000 description 3
- 241000256618 Trichogramma Species 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 235000013399 edible fruits Nutrition 0.000 description 3
- 238000005507 spraying Methods 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- 241000819999 Nymphes Species 0.000 description 2
- 244000134552 Plantago ovata Species 0.000 description 2
- 235000003421 Plantago ovata Nutrition 0.000 description 2
- 240000004064 Poterium sanguisorba Species 0.000 description 2
- 239000009223 Psyllium Substances 0.000 description 2
- 244000088401 Pyrus pyrifolia Species 0.000 description 2
- 235000001630 Pyrus pyrifolia var culta Nutrition 0.000 description 2
- 235000011572 Pyrus ussuriensis Nutrition 0.000 description 2
- 241000607479 Yersinia pestis Species 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000003755 preservative agent Substances 0.000 description 2
- 230000002335 preservative effect Effects 0.000 description 2
- 229940070687 psyllium Drugs 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 241000238876 Acari Species 0.000 description 1
- 241000256846 Apis cerana Species 0.000 description 1
- 241001048568 Apolygus lucorum Species 0.000 description 1
- 241000255749 Coccinellidae Species 0.000 description 1
- 235000015001 Cucumis melo var inodorus Nutrition 0.000 description 1
- 240000002495 Cucumis melo var. inodorus Species 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000258937 Hemiptera Species 0.000 description 1
- 241000255129 Phlebotominae Species 0.000 description 1
- 241001414857 Psyllidae Species 0.000 description 1
- 241000231139 Pyricularia Species 0.000 description 1
- 241001414989 Thysanoptera Species 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000012620 biological material Substances 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 244000144987 brood Species 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000002354 daily effect Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 230000009191 jumping Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Feed For Specific Animals (AREA)
Abstract
The invention provides a manual propagation method of psylla chinensis parasitic wasps and application thereof, wherein the method comprises the following steps: collecting the psylla chinensis nymphs which are parasitized by the psylla chinensis parasitism bees in a pear orchard and pear leaves together, carrying out artificial culture, mixing newly emerged psylla chinensis parasitism bee female and male adults, inoculating the newly emerged psylla chinensis parasitism bee female and male adults into an insect rearing cage for placing pear seedlings with four-age and five-age psylla chinensis nymphs, feeding the pear seedlings with honey water, mating and spawning, taking out the pear seedlings after 3 days, moving the pear seedlings to another insect rearing room for continuous rearing, observing the development state of the psylla chinensis nymphs thereon day by day, picking the psylla chinensis nymphs which are parasitized by the parasitism bees into a centrifugal tube, carrying out artificial culture, allowing the pear lice parasite bee female and male adults to be newly emerged, inoculating the newly emerged psylla chinensis parasitism bee female and male adults into a new insect rearing cage for the pear seedlings with the four-age and five-age psylla chinensis nymphs, feeding the pear lice water for spawning, mating and repeating the steps to obtain a continuous and stable number of the psylla chinensis parasitism.
Description
Technical Field
The invention belongs to the field of artificial feeding of natural enemy insects, and particularly relates to an artificial propagation method for psylla chinensis parasitic wasps and application thereof.
Background
Chinese pear psyllid (Cacopsylla chinensis) belongs to the family of hemiptera and psyllidae, is commonly called Chinese pear psyllid, and is a dangerous piercing-sucking pest which seriously damages pear trees. Although the body size is small and the eating quality is single, the pear tree can eat various pear tree varieties, and both adults and nymphs can be seriously harmed. The tree is damaged by mildew which is caused by weak tree vigor caused by direct piercing and sucking of adults and by indirect honeydew secretion of nymphs, so that the leaf surface is dry, and the quality and yield of fruits are seriously reduced. The direct damage and the indirect damage jointly influence the growth and the fruit quality of the pear trees. People have been adopting a method of spraying chemical pesticide to prevent and control the psylla chinensis for a long time, and abuse of the chemical pesticide not only causes the pest to generate drug resistance, but also causes the pollution of fruits, the reduction of the quantity of sales and the quantity of export.
Although there are various natural enemies of the pear psylla, such as lygus lucorum, sandfly, ladybug, thrips, predatory mites, parasitic wasps and the like, the small-leaved bees are one of the most important parasitic natural enemies of the pear psylla, and two kinds of small-leaved bees with very good effect of controlling the pear psylla are found in research, namely the small-leaved bees of the pear psylla (psylla insidiosus Crawgord) and the small-leaved bees of the wood louse (Prionitus mitratus Dal-nan), and the research on the small-leaved bees is limited to the aspects of morphological characteristics, life habits, protective and utilization measures and the like. The Pimpinella chinensis Queensis occurs for 6-7 generations in 1 year in Shandong province in China, and larvae or pupae live through the winter in the Pimpinella chinensis nymph body. In the next 3-4 months, the pear psyllids overwinter lay eggs, the eggs hatch to be damaged by nymphs in the last 4 months, the apis cerana adults in the middle 4 months eclosion lay eggs in the nymphs of the pear psyllids of 1-2 years, and the hosts die soon and become dark brown. In spring and autumn, the jumping wasps 25d finish 1 generation, and 15-18d finish 1 generation in summer. In the orchard without spraying or spraying less pesticide, the parasitic rate can reach more than 98 percent. The trichogramma psyllid is widely distributed in the southern Lu area, is an important parasitic bee of psyllid pyricularia, and has the same life habit as the trichogramma psyllid. At present, an indoor artificial feeding method for psylla chinensis parasitoid bees is still blank.
Disclosure of Invention
The invention provides an indoor feeding method for psylla chinensis parasitic wasps.
The indoor feeding method of the psylla chinensis parasitoid wasps provided by the invention comprises the following steps:
collecting the psylla chinensis nymphs parasitized by the egg laying of the psylla chinensis parasitic wasps in a pear orchard (the parasitized psylla chinensis nymphs are red and swollen in the initial stage and become brown) and pear leaves, placing the parasitized psylla chinensis nymphs in a centrifuge tube for artificial culture, observing the development and emergence of the psylla chinensis parasitic wasps every day, placing pear seedlings with four-year and five-year psylla chinensis nymphs in an insect cage, mixing the newly-formed male and female pyriella chinensis parasitic wasps into the insect cage, feeding with honey water, mating for egg laying, taking out the pear seedlings after 3 days, moving to another insect chamber for continuous culture, observing the development state of the psylla chinensis nymphs day by day, picking up the single heads of the psylla chinensis nymphs parasitized by the parasitic wasps into a centrifuge tube, artificially culturing, observing the emergence every day, mixing the newly-formed male and female pyriella chinensis parasitic pyriella chinensis nymphs into a new four-year and five-year pear chinensis brood pear seedlings, feeding with honey water, mating and laying eggs, and repeating the steps to obtain the psylla chinensis parasitism bees with continuous and stable quantity.
In the method, the centrifugal tube filled with the psylla chinensis nymphs parasitized by parasitic wasps is used for plugging the tube opening with a cotton wool ball to keep ventilation;
the centrifugal tube can be a 1.5mL or 2mL centrifugal tube;
the artificial culture is carried out in an artificial climate incubator, and the conditions of the artificial climate incubator are set as follows: temperature 26 ± 1 ℃, RH70 ± 5%, L: d ═ 16: 8;
the pear seedlings with four-age and five-age psylla chinensis nymphs can be prepared by the following method:
1) planting pear seedlings: sowing pear seeds subjected to germination accelerating treatment, planting the pear seeds germinated on the same day by using a flowerpot with the same specification and a matrix with the mass ratio of nutrient soil to vermiculite being 1:1, and culturing the pear seeds in an insect-raising room at 26 +/-1 ℃ and RH 65 +/-5% under the illumination condition all day long;
2) feeding pear psylla: putting pear seedlings with 5-6 leaves into an incubator, inoculating paired psyllid adults to enable the pear seedlings to lay eggs, and obtaining pear seedlings with psyllid nymphs of four-year-old and five-year-old psyllids after the pear lice eggs are incubated and the nymphs grow to four-year-old to five-year-old;
wherein, pesticide is not used in the planting process of the pear seedlings, and watering is carried out for 2 times per week;
planting pear seedlings every week;
the newly emerged psylla chinensis parasitoid wasps refer to psylla chinensis parasitoids within 12-48 hours after emergence;
soaking the absorbent cotton balls in honey water with the volume concentration of 20% to provide nutrition for adult psylla chinensis parasitoid wasps;
the cotton ball is replaced 1-2 times daily.
The psylla chinensis parasitoid wasp can be psylla chinensis dancing wasp.
The application of the method in the artificial propagation of the psylla chinensis parasitic wasps also belongs to the protection scope of the invention.
The obtained psylla chinensis parasitic wasp can be used for experimental research and is used for controlling psylla chinensis by being released in a pear orchard.
The method is simple and convenient to operate, low in production cost and small in occupied space, is suitable for indoor mass propagation of the psylla chinensis leaps for experimental research and release of the psylla chinensis leaps in a pear garden to control the psylla chinensis, and avoids adverse effects on environment and food safety caused by pesticide residues due to the use of chemical agents.
Drawings
Figure 1 shows age preference of psylla pyricularis parasitoids against psylla pyricularis;
fig. 2 is a graph of survival rate of parasitic bees with different substances as nutrients over time.
Detailed Description
The present invention will be described below with reference to specific examples, but the present invention is not limited thereto.
The experimental methods used in the following examples are all conventional methods unless otherwise specified; reagents, biomaterials, etc. used in the following examples are commercially available unless otherwise specified.
1. Apparatus and materials
Incubator (RXZ Intelligent artificial climate box Ningbo Jiangnan instrument factory)
Analytical balance (Danfo instrument Beijing GmbH)
Nutrient solution (20% honey water), insect cage (25cm × 25cm × 35cm), and plastic flowerpot
Agar strips (Chinese medicine), gauze (60 mesh), absorbent cotton, brush pen, tweezers, culture dish, scissors, vermiculite, flower fertilizer, insect needle, insect suction pipe, white plastic basin (40cm is multiplied by 50cm)
2. Artificial breeding method
2.1, pear seedling planting: sowing the pear seeds subjected to germination accelerating treatment, planting yellow-crown pear seeds germinated on the same day by using flowerpots of the same specification and a ratio of nutrient soil to vermiculite of 1:1, and culturing in an insect room at 26 +/-1 ℃ and RH 65 +/-5% under the whole-day illumination condition. No pesticide was used and the water was applied 2 times per week.
2.2, feeding the psyllids: collected in a Beijing mountain pear orchard, inoculated on pear seedlings, cultured and propagated for multiple generations in an indoor illumination incubator (26 +/-1 ℃, RH70 +/-5%, L: D ═ 16: 8). And collecting the pear psyllids outdoors in spring and summer every year for reseeding, and keeping the quality and activity of the population.
2.3, feeding the psylla chinensis benth: collected in a Beijing Yangshan pear garden, and the psylla chinensis nymphs parasitized by the egg laying of the psylla chinensis parasitism bees and pear leaves are collected to a laboratory. Picking parasitized psylla chinensis stiff insects into a 10mL centrifuge tube by using an insect needle, plugging the tube opening by using a cotton wool ball, keeping ventilation, and placing the tube opening into a climatic incubator (the temperature is 26 +/-1 ℃, RH70 +/-5%, and L: D is 16: 8) for eclosion. After eclosion, single head of Lophocarpus pyrifolia parasitic wasp (within 24 h) is transferred into finger-shaped tube, and absorbent cotton ball soaked with 20% volume of honey water is placed for nutrition, and the cotton ball is replaced 1-2 times per day.
Planting pear seedlings every week, and planting 1-2 pear seedlings in each plastic flowerpot. 10 strong pear seedlings with 5-6 leaves are selected to be placed into a culture box (the temperature is 26 +/-1 ℃, RH70 +/-5 percent, and L: D is 16: 8), and 10 pairs of adult pear psyllids are inoculated to enable the adult pear psyllids to lay eggs. After three weeks, the eggs of the psyllium pyricularis are incubated, the nymphs develop to about five years of age, the pear seedlings with the psyllium pyricularis nymphs are transferred to a new insect rearing cage (25cm multiplied by 35cm), newly emerged (12-48 hours) parasitoid wasps 5 which are not laid eggs are butt-jointed into the insect rearing cage, and the parasitoids are fed by using a degreasing cotton ball soaked with honey water with the volume concentration of 20%. Taking out pear seedlings after 3 days, moving the pear seedlings to an insect culture room for continuous cultivation, observing the development state of psylla chinensis nymphs on the pear seedlings day by day, picking the parasitized psylla chinensis nymphs into a centrifuge tube, placing the centrifuge tube in an artificial climate incubator (26 +/-1 ℃, 70 +/-5% of RH and 16: 8 of photoperiod L: D), and waiting for emergence. Transferring eclosion psylla chinensis parasitoid bees (within 24 h) into the finger-shaped tube, mixing male and female imagoes, providing nutrition for the absorbent cotton balls soaked by 20% of honey water, observing every day, and timely supplementing the absorbed absorbent cotton balls with honey water; and (3) the copulated female trichogramma is again inoculated into a new insect rearing cage with four-age or five-age psylla chinensis nymph pear seedlings, offspring is continuously bred, and the breeding number of parasitic wasps is enlarged.
The parasitic wasp bred by the method has high parasitic rate (the average parasitic rate reaches 50%) and emergence rate (the emergence rate is more than 90%), can be stably bred, and is successfully bred for more than 6 generations in the laboratory.
Example 2
3.1 preference of Lophocarpus pyrifolia parasitic wasps on different instars of Lophocarpus pyrifolia
Preparing a 9cm culture dish, preparing 2% water agar, pouring into the culture dish, wherein the thickness is about 3mm, taking the third and fourth leaves of the pear seedling, inserting the petioles into the agar for moisture preservation, respectively connecting 10 heads of nymphs of psyllids pyricularis from one age to five ages on the leaves, introducing a pair of parasitic bees which are eclosion for 24 hours, sealing the openings by using a preservative film, and pricking 50 small holes on the preservative film by using a No. 1 insect needle for ventilation. And (3) putting the pear psyllids into an incubator, removing parasitic wasps after 24 hours, continuously culturing, and observing the number of parasitic pear psyllids at each age, wherein the number of parasitic wasps is 8. As shown in fig. 1, it can be seen from fig. 1 that the parasitic wasps are more preferable to parasitize psyllid nymphs of four and five ages than to parasitize psyllid nymphs of one to three ages. Therefore, the four to five-year-old psylla chinensis nymphs are selected as parasitic wasp hosts, and a better propagation effect can be achieved.
Figure 1 shows the age preference of psylla chinensis parasitoids on psylla chinensis.
3.2 Effect of nutritional conditions on Liriopsis chinensis parasitic wasp adult Life
50-head psylla chinensis parasitic bees within 24 hours of emergence are respectively inoculated into three plastic pipes with the length of 10cm and the diameter of 2cm, and then are respectively placed into degreased cotton balls soaked with distilled water, honey water with the volume concentration of 10% and honey water with the volume concentration of 20%, so that nutrition is provided, the death number of the parasitic bees is recorded by observing every day, and the dried cotton balls are timely supplemented with nutritional ingredients. As a result, as shown in fig. 2, it was found that parasitic wasps were alive for a longer time while 20% of honey water was supplied as nutrition, and almost all of the parasitic wasps died within three days in the case of supplying only water. Therefore, the parasitic wasp can be better raised by selecting 20% of honey water as the food of the parasitic wasp.
Fig. 2 is a graph of survival rate of parasitic bees with different substances as nutrients over time.
Claims (7)
1. An indoor breeding method of psylla chinensis parasitoid wasps comprises the following steps:
collecting psylla chinensis nymphs which are already spawned and parasitized by psylla chinensis parasitism bees in a pear orchard together with pear leaves, artificially culturing, placing pear seedlings with four-age and five-age psylla chinensis nymphs in insect cages after the psylla chinensis parasitism bees develop and emerge, mixing the newly emerged psylla chinensis parasitism bee hermaphrodite and male imagoes and inoculating the imagoes and female imagoes into the insect cages, feeding with honey water, mating for oviposition, taking out pear seedling after 3 days, transferring to another insect-raising room for further cultivation, observing the development state of the psylla chinensis nymphs day by day, picking one end of the psylla chinensis nymphs parasitized by the parasitic wasps to a centrifugal tube, artificially culturing, allowing emergence, mixing newly emerged male and female imagoes of Lopholidae chinensis parasitic wasps, inoculating into new insect cage with four-year-old and five-year-old Lopholidae chinensis nymphs, feeding with honey water, mating and laying eggs, and repeating the steps to obtain the psylla chinensis parasitism bees with continuous and stable quantity.
2. The method of claim 1, wherein: the centrifugal pipe filled with the psylla chinensis nymphs parasitized by the parasitic wasps is plugged with a degreasing cotton ball to keep ventilation;
the artificial culture is carried out in an artificial climate incubator,
the conditions of the artificial climate incubator are set as follows: temperature 26 ± 1 ℃, RH70 ± 5%, L: d ═ 16: 8.
3. the method according to claim 1 or 2, characterized in that: the pear seedlings with four-age and five-age psylla chinensis nymphs are prepared by the following method:
1) planting pear seedlings: sowing pear seeds subjected to germination accelerating treatment, planting the pear seeds germinated on the same day by using a flowerpot with the same specification and a matrix with the mass ratio of nutrient soil to vermiculite being 1:1, and culturing the pear seeds in an insect-raising room at 26 +/-1 ℃ and RH 65 +/-5% under the illumination condition all day long;
2) feeding pear psylla: putting pear seedlings with 5-6 leaves into an incubator, inoculating paired psyllid adults to enable the pear seedlings to lay eggs, and obtaining the pear seedlings with psyllid nymphs of four-year-old and five-year-old psyllids after the pear lice eggs are incubated and the nymphs grow to four-year-old to five-year-old.
4. The method according to any one of claims 1-3, wherein: the newly emerged psylla chinensis parasitoid is psylla chinensis parasitoid within 12-48 hours after emergence.
5. The method according to any one of claims 1-4, wherein: the absorbent cotton balls are soaked by honey water with the volume concentration of 20% to provide nutrition for the adult psylla chinensis parasitoid wasps.
6. The method according to any one of claims 1-5, wherein: the psylla chinensis parasitoid is psylla chinensis dancing bee.
7. Use of the method according to any one of claims 1 to 6 for the artificial propagation of psyllid parasitoids.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911200921.1A CN110959581A (en) | 2019-11-29 | 2019-11-29 | Artificial propagation method for psylla chinensis parasitic wasps and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201911200921.1A CN110959581A (en) | 2019-11-29 | 2019-11-29 | Artificial propagation method for psylla chinensis parasitic wasps and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110959581A true CN110959581A (en) | 2020-04-07 |
Family
ID=70032122
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201911200921.1A Pending CN110959581A (en) | 2019-11-29 | 2019-11-29 | Artificial propagation method for psylla chinensis parasitic wasps and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110959581A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112790163A (en) * | 2021-02-05 | 2021-05-14 | 中国热带农业科学院环境与植物保护研究所 | Method for feeding small-head psylla mori |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5745194A (en) * | 1980-09-01 | 1982-03-13 | Nippon Tokushu Noyaku Seizo Kk | Organic phosphoric ester, its preparation and insecticide, acaricide or nematocide containing the same |
| CN104621052A (en) * | 2015-02-11 | 2015-05-20 | 广东省昆虫研究所 | Diaphorina citri Kuwayama parasitism natural energy scale breeding method |
| CN105613164A (en) * | 2014-10-29 | 2016-06-01 | 韩浩良 | Novel technique for treatment of psylla chinensis in pear orchard in winter |
| CN106954592A (en) * | 2017-04-14 | 2017-07-18 | 华南农业大学 | A kind of Ali eats the biological control method of lice encyrtid |
-
2019
- 2019-11-29 CN CN201911200921.1A patent/CN110959581A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS5745194A (en) * | 1980-09-01 | 1982-03-13 | Nippon Tokushu Noyaku Seizo Kk | Organic phosphoric ester, its preparation and insecticide, acaricide or nematocide containing the same |
| CN105613164A (en) * | 2014-10-29 | 2016-06-01 | 韩浩良 | Novel technique for treatment of psylla chinensis in pear orchard in winter |
| CN104621052A (en) * | 2015-02-11 | 2015-05-20 | 广东省昆虫研究所 | Diaphorina citri Kuwayama parasitism natural energy scale breeding method |
| CN106954592A (en) * | 2017-04-14 | 2017-07-18 | 华南农业大学 | A kind of Ali eats the biological control method of lice encyrtid |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112790163A (en) * | 2021-02-05 | 2021-05-14 | 中国热带农业科学院环境与植物保护研究所 | Method for feeding small-head psylla mori |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101331868B (en) | Artificial propagation method of minute pirate bugs | |
| CN101390505B (en) | Encarsia Sophia artificial propagation production method | |
| CN106954592B (en) | Large-scale breeding method for Alliid lice spikemoss | |
| EA022197B1 (en) | Composition for biological pest control on plants, use thereof, a container for rearing a phytoseiid predatory mite, and methods for rearing a phytoseiid predatory mite and for biological pest control on a crop | |
| Atwal | Influence of temperature, photoperiod, and food on the speed of development, longevity, fecundity, and other qualities of the Diamond-Back moth Plutella Maculipennis (Curtis)(Lepidoptera: Tineidae). | |
| CN112205358B (en) | Indoor population propagation method for spodoptera frugiperda | |
| CN110235860B (en) | Large-scale feeding method of Gray aphids | |
| CN102870742A (en) | Large-scale artificial feeding method for eretmocerus hayati | |
| CN103960203A (en) | Method for breeding encarsia sophia drones by means of eretmocerus hayati | |
| CN112042598B (en) | Whole set method for breeding harmonia axyridis | |
| CN103503836A (en) | Plutella xylostella cotesia rubecula artificial propagation production method | |
| CN104957107B (en) | A kind of method of interior raising Propylaea japonica | |
| Kostiainen et al. | Egg-harvesting allows large scale rearing of Amblyseius finlandicus (Acari: Phytoseiidae) in the laboratory | |
| CN113519468B (en) | Method for feeding phyllotreta striolata in indoor generation | |
| JP2013252123A (en) | Method and container for rearing natural enemy insect of spider mite | |
| Murungi et al. | Effect of African nightshade species (Solanaceae) on developmental time and life table parameters of Tetranychus evansi (Acari: Tetranychidae) | |
| CN103766324A (en) | Method for biologically preventing green plant bugs by peristenus spretus in winter jujube orchard | |
| CN110959581A (en) | Artificial propagation method for psylla chinensis parasitic wasps and application thereof | |
| CN104604809A (en) | Biological control method using encarsia formosa for controlling tobacco whitefly | |
| CN112586453B (en) | Simple feeding method for predatory mites | |
| CN109197786B (en) | Method for breeding large amount of semi-closed curvicauda curvularia indoors | |
| CN110896927A (en) | Indoor artificial propagation method for psylla chinensis | |
| CN113508783A (en) | Method for breeding large quantities of Aphidius aurantiaca by using cotton seedlings and MED bemisia tabaci | |
| CN113875704A (en) | Indoor propagation method for hornet | |
| CN107581163B (en) | Method for feeding cryptopterus gracilis by using tetranychus cinnabarinus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20200407 |
|
| RJ01 | Rejection of invention patent application after publication |