CN106259208B - A kind of method that tobacco plant repeatedly breeds aphidius gifuensis - Google Patents
A kind of method that tobacco plant repeatedly breeds aphidius gifuensis Download PDFInfo
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- CN106259208B CN106259208B CN201610641438.7A CN201610641438A CN106259208B CN 106259208 B CN106259208 B CN 106259208B CN 201610641438 A CN201610641438 A CN 201610641438A CN 106259208 B CN106259208 B CN 106259208B
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- 235000002637 Nicotiana tabacum Nutrition 0.000 title claims abstract description 33
- 241001305034 Aphidius gifuensis Species 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 title claims abstract description 22
- 244000061176 Nicotiana tabacum Species 0.000 title claims abstract description 9
- 241001124076 Aphididae Species 0.000 claims abstract description 155
- 235000019504 cigarettes Nutrition 0.000 claims abstract description 92
- 238000009395 breeding Methods 0.000 claims abstract description 29
- 230000001488 breeding effect Effects 0.000 claims abstract description 29
- 241000196324 Embryophyta Species 0.000 claims abstract description 21
- 241000208125 Nicotiana Species 0.000 claims description 27
- 238000009792 diffusion process Methods 0.000 claims 1
- 241000607479 Yersinia pestis Species 0.000 abstract description 3
- 235000001674 Agaricus brunnescens Nutrition 0.000 abstract description 2
- 230000002265 prevention Effects 0.000 description 5
- 241000700605 Viruses Species 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 2
- 230000013011 mating Effects 0.000 description 2
- 244000045947 parasite Species 0.000 description 2
- 230000007115 recruitment Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241001600407 Aphis <genus> Species 0.000 description 1
- 241001425390 Aphis fabae Species 0.000 description 1
- 235000015001 Cucumis melo var inodorus Nutrition 0.000 description 1
- 240000002495 Cucumis melo var. inodorus Species 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241000819999 Nymphes Species 0.000 description 1
- 241000180219 Sitobion avenae Species 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 230000034303 cell budding Effects 0.000 description 1
- 238000012272 crop production Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000001939 inductive effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 238000003976 plant breeding Methods 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 230000027272 reproductive process Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Botany (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Catching Or Destruction (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention belongs to the technical field of biological control of agriculture and forestry pest, and in particular to a kind of method that tobacco plant repeatedly breeds aphidius gifuensis, including cigarette aphid host plant are cultivated, numerous bee for the first time, second of numerous bee, the numerous bee of third time, the 4th numerous bee, make full use of the root system of cigarette strain prosperity, promote young leaves to sprout and mushroom out, so that cigarette strain is reused, do not need to carry out secondary nursery and transplanting, saving of work and time greatly improves the efficiency of aphidius gifuensis breeding.
Description
Technical field
The invention belongs to the technical field of biological control of agriculture and forestry pest, and in particular to a kind of tobacco plant repeatedly breeds cigarette
The method of aphid parasite.
Background technique
Aphid is one of the primary pest in farm-forestry crop production, and the harm of aphid is particularly acute in leaf tobacco production, main
Tobacco leaf juice is sucked on tobacco leaf back and tender stem with adult and nymph, cause cigarette strain to grow short and small, nutritional imbalance, influence cigarette
The normal growth and development of strain, the easy generation for inducing the diseases such as dark mildew of the honeydew of aphid secretion, while aphid are also propagated a variety of
Tobacco virus causes the prevalence of tobacco virus；Mainly there are chemical prevention and physical control to aphid, chemical prevention relies on agriculture
Medicine eliminates aphid, will cause bio-diversity reduction, destroys ecological environment, and pollution of agricultural products influences food safety, and aphid resists
Pharmacological property enhancing, control efficiency decline, physical control relies primarily on yellow plate prevention and control winged aphids, when winged aphids colonizes on plant
When, prevention and control effect that yellow plate has just scattered and disappeared to aphid.
In Agro-ecology development, biological prevention is anti-one of the most effective approach that eliminates aphis, aphidius gifuensis
It is the advantage parasite of cigarette aphid, is distributed throughout our country, ovum is originated in feeding juice in aphid body and killed by aphidius gifuensis
Dead or control aphid, breeds the offspring of oneself, it has good control efficiency to cigarette aphid, while to grain aphid, wheat y-bend
The aphids such as aphid, bean aphid, cotten aphid also have certain control effect, currently, aphidius gifuensis artificial breeding technology, host plant are most
For tobacco, there are two types of mating systems: seedling breeding bee method and strain breeding bee method, their breeding cycle is too long, and it is existing this
Both of which can only be bred once, and to breed second must re-start nursery and transplanting cigarette strain, and urgent need is studied a kind of steady
The mating system of the fixed aphidius gifuensis for efficiently shortening numerous bee period.
Summary of the invention
In view of the deficiencies of the prior art, the present invention provides a kind of stability and high efficiency, can shorten the breeding cycle of aphidius gifuensis,
Reduce efficient numerous bee method of recruitment cost.
The technical scheme of the present invention is realized as follows: a kind of method that tobacco plant repeatedly breeds aphidius gifuensis, breeding
The step of aphidius gifuensis, is as follows:
1) cigarette aphid host plant is cultivated: the host plant tobacco seedlings of cigarette aphid are cultivated using floating seedlings method；
2) first time numerous bee:
(1) connect the numerous aphid of aphid: tobacco seedlings when be cultivated in step 1) it is long to 4 to 5 true leaves when start to connect aphid, every plant of tobacco seedlings connect aphid
2 to 3, cigarette aphid is allowed voluntarily to spread breeding；
(2) the numerous bee of bee is connect:, i.e., will be with aphid after cigarette aphid is evenly distributed and breeds in right amount after connecing aphid 5 to 7 days in step (1)
Tobacco seedlings are transplanted in numerous bee greenhouse, and band aphid tobacco seedlings plant/line spacing is 40cm, and transplanting is followed by bee in 20 to 25 days, and tobacco seedlings have been grown at this time
At cigarette strain, a large amount of stiff aphids and aphidius gifuensis can be formed within 15 to 20 days after connecing bee；
3) second of numerous bee:
(1) connect the numerous aphid of aphid: cigarette strain root system is undeveloped when numerous bee for the first time, while the harm by cigarette aphid, cigarette strain and cigarette strain
Blade is all not too large, and when numerous bee initially forms a large amount of stiff aphids for the first time, the harm of cigarette aphid gradually mitigates, cigarette strain root system also by
Gradually flourishing, cigarette strain top begins to send out young leaves, will be covered with the blade removal of stiff aphid at this time, the young leaves on top is left and taken, due to root system
Prosperity, the speed of growth of young leaves quickly, connect aphid on young leaves, and every plant of cigarette strain connects aphid 20 to 30, and cigarette aphid is allowed voluntarily to spread breeding；
(2) connect the numerous bee of bee: after connecing aphid 10 to 15 days in step (1), i.e., cigarette aphid is evenly distributed and mass propagation is followed by bee,
A large amount of stiff aphids and aphidius gifuensis can be formed within 15 to 20 days after connecing bee；
4) the numerous bee of third time:
(1) connect the numerous aphid of aphid: cigarette strain root system still locates the flourishing phase after second of numerous bee, and cigarette strain top still can issue newly
Leaf will be covered with the blade removal of stiff aphid at this time, leave and take the young leaves on top, due to well developed root system, the speed of growth of young leaves quickly,
Aphid is connect on young leaves, every plant of cigarette strain connects aphid 20 to 30, and cigarette aphid is allowed voluntarily to spread breeding；
(2) connect the numerous bee of bee: after connecing aphid 10 to 15 days in step (1), i.e., cigarette aphid, which is evenly distributed and breeds in right amount, is followed by bee,
A large amount of stiff aphids and aphidius gifuensis can be formed within 15 to 20 days after connecing bee；
5) the 4th numerous bee:
(1) connect the numerous aphid of aphid: cigarette strain is higher after the numerous bee of third time or has buddingged, and will not issue young leaves, but root again
System is still flourishing, and cigarette strain can be pinched in this case or be chopped off from middle part, and leave and take two blades, promote axillary bud sprouting and
Growth, grows to axillary bud blade, and two blade removals left and taken when by topping connect aphid, each axillary bud connects aphid on axillary bud blade
10 to 15, cigarette aphid is allowed voluntarily to spread breeding；
(2) connect the numerous bee of bee: after connecing aphid 10 to 15 days in step (1), i.e., cigarette aphid, which is evenly distributed and breeds in right amount, is followed by bee,
A large amount of stiff aphids and aphidius gifuensis can be formed within 15 to 20 days after connecing bee.
Numerous bee canopy temperature is 17 to 27 degrees Celsius, and humidity is 30% to 70%.
When the first time numerous bee, second of numerous bee, the numerous bee of third time and the 4th numerous bee connect bee, bee aphid ratio is 1:
50 to 1:100.
The present invention has following the utility model has the advantages that cigarette strain can recycle, and carries out repeatedly numerous bee using the cigarette strain after growing up,
It for the first time when numerous bee, is transplanted using band aphid, the repoductive time of cigarette aphid and the growth time of cigarette strain are put together, reduce host to the greatest extent
The cultivation time of plant greatly shortens numerous bee period；Stability and high efficiency does not need the cultivation repeatedly of host plant, reduces recruitment
Cost, saving of work and time.
Specific embodiment
A kind of the step of method that tobacco plant repeatedly breeds aphidius gifuensis, breeding aphidius gifuensis, is as follows: 1) cigarette aphid host
Plant cultivation: the host plant tobacco seedlings of cigarette aphid are cultivated using floating seedlings method；2) first time numerous bee: (1) the numerous aphid of aphid is connect: to step
1) start to connect aphid when tobacco seedlings length to 4 to 5 true leaves cultivated in, every plant of tobacco seedlings connect aphid 2 to 3, and cigarette aphid is allowed voluntarily to spread breeding；
(2) it connects the numerous bee of bee: after connecing aphid 5 to 7 days in step (1), i.e., after cigarette aphid is evenly distributed and breeds in right amount, will be transplanted with aphid tobacco seedlings
Into numerous bee greenhouse, band aphid tobacco seedlings plant/line spacing is 40cm, and transplanting is followed by bee in 20 to 25 days, and tobacco seedlings have grown up to cigarette strain at this time,
A large amount of stiff aphids and aphidius gifuensis can be formed within 15 to 20 days after connecing bee；3) second of numerous bee: (1) the numerous aphid of aphid is connect: numerous bee for the first time
When cigarette strain root system it is undeveloped, while the harm by cigarette aphid, cigarette strain and cigarette strain blade are all not too large, and when for the first time, numerous bee starts
When forming a large amount of stiff aphids, the harm of cigarette aphid gradually mitigates, and cigarette strain root system is also gradually flourishing, and cigarette strain top begins to send out young leaves, this
When will be covered with the blade removal of stiff aphid, leave and take the young leaves on top, due to well developed root system, the speed of growth of young leaves quickly, in young leaves
On connect aphid, every plant of cigarette strain connects aphid 20 to 30, and cigarette aphid is allowed voluntarily to spread breeding；(2) the numerous bee of bee is connect: to connect aphid 10 in step (1)
To after 15 days, i.e., cigarette aphid is evenly distributed and mass propagation is followed by bee, can form within 15 to 20 days a large amount of stiff aphids and cigarette aphid after connecing bee
Cocoon bee；4) the numerous bee of third time: (1) connect the numerous aphid of aphid: cigarette strain root system still locates the flourishing phase after second of numerous bee, and cigarette strain top is still
Young leaves can be issued, the blade removal of stiff aphid will be covered at this time, leaves and takes the young leaves on top, due to well developed root system, the growth of young leaves
Speed quickly, connects aphid on young leaves, and every plant of cigarette strain connects aphid 20 to 30, and cigarette aphid is allowed voluntarily to spread breeding；(2) the numerous bee of bee is connect: to
After connecing aphid 10 to 15 days in step (1), i.e., cigarette aphid, which is evenly distributed and breeds in right amount, is followed by bee, can be formed within 15 to 20 days after connecing bee
A large amount of deadlock aphids and aphidius gifuensis；5) the 4th numerous bee: (1) connect the numerous aphid of aphid: cigarette strain is higher or after the numerous bee of third time
Budding, young leaves will not be issued again, but root system is still flourishing, cigarette strain can be pinched in this case or chopped off from middle part, and leaves and takes
Two blades promote the sprouting and growth of axillary bud, grow to axillary bud blade, two blade removals left and taken when by topping, in armpit
Bud-leaf on piece connects aphid, and each axillary bud connects aphid 10 to 15, and cigarette aphid is allowed voluntarily to spread breeding；(2) the numerous bee of bee is connect: in step (1)
After connecing aphid 10 to 15 days, i.e., cigarette aphid, which is evenly distributed and breeds in right amount, is followed by bee, can form within 15 to 20 days a large amount of stiff aphids after connecing bee
And aphidius gifuensis.Numerous bee canopy temperature is 17 to 27 degrees Celsius, and humidity is 30% to 70%.The first time is numerous
When bee, second of numerous bee, the numerous bee of third time and the 4th numerous bee connect bee, bee aphid ratio is 1:50 to 1:100.
Use tobacco to have as the method for host plant breeding aphidius gifuensis in the prior art: seedling breeding bee method and strain are numerous
Bee method.Seedling breeding bee method is the method using floating seedlings, the tobacco seedlings grand cross phase be start within after planting 50 to 60 days to connect aphid into
The breeding of row cigarette aphid, starts to connect bee after cigarette aphid is largely formed within 10 to 15 days, can form within 15 to 20 days a large amount of deadlock aphids and at bee, entirely
Breeding cycle was at 80 to 90 days or so.Strain breeding bee method is started within i.e. after planting 70 to 80 days in tobacco seedlings length to 5 to 6 true leaves
Transplanting, when cigarette strain it is long to 9 to 12 true leaves when transplanting after connect within 25 to 30 days aphid, after 15 to 20 days introducing at bee, connect bee 15 to
It can produce within 20 days a large amount of stiff aphids and at bee, breeding cycle be at 120 days or more.
The breeding of host in aphidius gifuensis reproductive process and the growth of host plant are placed on same stage and carried out by the present invention,
Numerous bee period is greatly shortened, is shortened 45 to 85 days, meanwhile, the root system of cigarette strain prosperity is made full use of, young leaves is promoted to sprout simultaneously
It mushrooms out, cigarette strain is enable to reuse, do not need to carry out secondary nursery and transplanting, saving of work and time greatly improves cigarette aphid
The efficiency of cocoon bee breeding.
Claims (3)
1. a kind of method that tobacco plant repeatedly breeds aphidius gifuensis, which is characterized in that the step of breeding aphidius gifuensis is as follows:
1) cigarette aphid host plant is cultivated: the host plant tobacco seedlings of cigarette aphid are cultivated using floating seedlings method；
2) first time numerous bee:
(1) connect the numerous aphid of aphid: tobacco seedlings when be cultivated in step 1) it is long to 4 to 5 true leaves when start to connect aphid, every plant of tobacco seedlings connect aphid 2 to 3
Head allows cigarette aphid voluntarily to spread breeding；
(2) the numerous bee of bee is connect:, i.e., will be with aphid tobacco seedlings after cigarette aphid is evenly distributed and breeds in right amount 5 to 7 days after connecing aphid in step (1)
It is transplanted in numerous bee greenhouse, band aphid tobacco seedlings plant/line spacing is 40cm, connects bee within 20 to 25 days after transplanting, tobacco seedlings have grown up to cigarette at this time
Strain can form a large amount of stiff aphids and aphidius gifuensis for 15 to 20 days after connecing bee；
3) second of numerous bee:
(1) connect the numerous aphid of aphid: cigarette strain root system is undeveloped when numerous bee for the first time, while the harm by cigarette aphid, cigarette strain and cigarette strain blade
All not too large, when numerous bee initially forms a large amount of stiff aphids for the first time, the harm of cigarette aphid gradually mitigates, and cigarette strain root system is also gradually sent out
It reaching, cigarette strain top begins to send out young leaves, will be covered with the blade removal of stiff aphid at this time, the young leaves on top is left and taken, due to well developed root system,
The speed of growth of young leaves quickly, connects aphid on young leaves, and every plant of cigarette strain connects aphid 20 to 30, and cigarette aphid is allowed voluntarily to spread breeding；
(2) connect the numerous bee of bee: 10 to 15 days after connecing aphid in step (1), i.e., cigarette aphid is evenly distributed and mass propagation is followed by bee, connects bee
A large amount of stiff aphids and aphidius gifuensis can be formed within 15 to 20 days afterwards；
4) the numerous bee of third time:
(1) connect the numerous aphid of aphid: cigarette strain root system still locates the flourishing phase after second of numerous bee, and cigarette strain top still can issue young leaves,
The blade removal of stiff aphid will be covered at this time, leave and take the young leaves on top, due to well developed root system, the speed of growth of young leaves quickly, new
Aphid is connect on leaf, every plant of cigarette strain connects aphid 20 to 30, and cigarette aphid is allowed voluntarily to spread breeding；
(2) connect the numerous bee of bee: 10 to 15 days after connecing aphid in step (1), i.e., cigarette aphid, which is evenly distributed and breeds in right amount, is followed by bee, connects bee
A large amount of stiff aphids and aphidius gifuensis can be formed within 15 to 20 days afterwards；
5) the 4th numerous bee:
(1) connect the numerous aphid of aphid: cigarette strain is higher after the numerous bee of third time or has buddingged, and will not issue young leaves again, but root system is still
It is so flourishing, cigarette strain is pinched or is chopped off from middle part, and leaves and takes two blades, promotes the sprouting and growth of axillary bud, to axillary bud blade
Grow, will topping when two blade removals leaving and taking, aphid is connect on axillary bud blade, each axillary bud connects aphid 10 to 15, allows cigarette aphid
Voluntarily diffusion breeding；
(2) connect the numerous bee of bee: 10 to 15 days after connecing aphid in step (1), i.e., cigarette aphid, which is evenly distributed and breeds in right amount, is followed by bee, connects bee
A large amount of stiff aphids and aphidius gifuensis can be formed within 15 to 20 days afterwards.
2. the method that tobacco plant according to claim 1 repeatedly breeds aphidius gifuensis, which is characterized in that numerous bee
Canopy temperature is 17 to 27 degrees Celsius, and humidity is 30% to 70%.
3. the method that tobacco plant according to claim 1 repeatedly breeds aphidius gifuensis, which is characterized in that described
When primary numerous bee, second of numerous bee, the numerous bee of third time and the 4th numerous bee connect bee, bee aphid ratio is 1:50 to 1:100.
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107455341A (en) * | 2017-08-01 | 2017-12-12 | 中国烟草总公司福建省公司 | A kind of aphidius gifuensis mating system of regulation and control host crop growth |
| CN107372372B (en) * | 2017-09-08 | 2020-09-08 | 云南省烟草公司红河州公司 | Method for reproducing myzus persicae and aphidius gifuensis by regenerating tobacco plants |
| CN107371764A (en) * | 2017-09-08 | 2017-11-24 | 云南省烟草公司楚雄州公司 | A kind of method that wet tobacco seedlings breeding cigarette aphid and aphidius gifuensis are floated using regeneration |
| CN109832229A (en) * | 2019-02-28 | 2019-06-04 | 山东省果树研究所 | A kind of device and application method detecting the remote diffusivity of entomopathogenic nematode |
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| CN101664017A (en) * | 2009-09-30 | 2010-03-10 | 云南省烟草农业科学研究院 | Method for propagating sulzer braconid flies |
| KR101044051B1 (en) * | 2010-12-23 | 2011-06-23 | 주식회사 세실 | Filler composition for breeding natural enemies, breeding composition for natural enemies, and controlling composition for insect pests comprising expanded chaff |
| CN104823923A (en) * | 2015-03-04 | 2015-08-12 | 云南绿叶生防科技有限公司 | Breeding method for preventing aphidius gifuensis species from degrading |
| CN105165313A (en) * | 2015-07-24 | 2015-12-23 | 中国烟草总公司四川省公司 | Method for breeding aphidius gifuensis within short cycle |
| CN105519496A (en) * | 2016-02-16 | 2016-04-27 | 弥渡格瑞生物科技有限责任公司 | Natural diffusion myzus persicae vaccination method |
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- 2016-08-08 CN CN201610641438.7A patent/CN106259208B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101664017A (en) * | 2009-09-30 | 2010-03-10 | 云南省烟草农业科学研究院 | Method for propagating sulzer braconid flies |
| KR101044051B1 (en) * | 2010-12-23 | 2011-06-23 | 주식회사 세실 | Filler composition for breeding natural enemies, breeding composition for natural enemies, and controlling composition for insect pests comprising expanded chaff |
| CN104823923A (en) * | 2015-03-04 | 2015-08-12 | 云南绿叶生防科技有限公司 | Breeding method for preventing aphidius gifuensis species from degrading |
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