CN104823923A - Breeding method for preventing aphidius gifuensis species from degrading - Google Patents
Breeding method for preventing aphidius gifuensis species from degrading Download PDFInfo
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- aphidius gifuensis
- aphid
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- stiff aphid
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- 241001305034 Aphidius gifuensis Species 0.000 title claims abstract description 330
- 238000009395 breeding Methods 0.000 title claims abstract description 104
- 230000000593 degrading effect Effects 0.000 title abstract 3
- 241001124076 Aphididae Species 0.000 claims abstract description 196
- 230000001488 breeding effect Effects 0.000 claims abstract description 100
- 230000013011 mating Effects 0.000 claims abstract description 65
- 238000012360 testing method Methods 0.000 claims abstract description 56
- 238000012216 screening Methods 0.000 claims abstract description 30
- 241000256844 Apis mellifera Species 0.000 claims abstract description 29
- 230000000694 effects Effects 0.000 claims abstract description 23
- 235000019504 cigarettes Nutrition 0.000 claims description 54
- 238000000338 in vitro Methods 0.000 claims description 53
- 230000006866 deterioration Effects 0.000 claims description 24
- 230000015572 biosynthetic process Effects 0.000 claims description 14
- 238000000034 method Methods 0.000 claims description 14
- 239000004677 Nylon Substances 0.000 claims description 13
- 241000973497 Siphonognathus argyrophanes Species 0.000 claims description 13
- 229920001778 nylon Polymers 0.000 claims description 13
- 230000036541 health Effects 0.000 claims description 11
- 229920000742 Cotton Polymers 0.000 claims description 10
- 238000005286 illumination Methods 0.000 claims description 10
- 230000003071 parasitic effect Effects 0.000 claims description 10
- 239000004576 sand Substances 0.000 claims description 10
- 239000006188 syrup Substances 0.000 claims description 10
- 235000020357 syrup Nutrition 0.000 claims description 10
- 239000000575 pesticide Substances 0.000 claims description 6
- 238000000746 purification Methods 0.000 claims description 5
- 230000032669 eclosion Effects 0.000 abstract description 8
- 241000721621 Myzus persicae Species 0.000 abstract 3
- 238000002474 experimental method Methods 0.000 description 5
- 241000196324 Embryophyta Species 0.000 description 4
- 230000015556 catabolic process Effects 0.000 description 4
- 238000006731 degradation reaction Methods 0.000 description 4
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000009400 out breeding Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 241000238631 Hexapoda Species 0.000 description 2
- 241000208125 Nicotiana Species 0.000 description 2
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- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 235000015001 Cucumis melo var inodorus Nutrition 0.000 description 1
- 240000002495 Cucumis melo var. inodorus Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 230000001364 causal effect Effects 0.000 description 1
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- 235000013601 eggs Nutrition 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
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- 210000000056 organ Anatomy 0.000 description 1
- 230000007105 physical stamina Effects 0.000 description 1
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Abstract
The invention discloses a breeding method for preventing aphidius gifuensis species from degrading. The breeding method consists the steps: bee species collection: collecting mummified aphids formed by artificial breeding of aphidius gifuensis and mummified aphids formed by field aphidius gifuensis, and storing the aphidius gifuensis in test tubes; bee species cultivation: placing the test tubes in an arterial climate box for cultivation, purifying the aphidius gifuensis after the mummified aphids in the test tubes are hatched into aphidius gifuensis; aphidius gifuensis mating: mating the artificially bred aphidius gifuensis and the field aphidius gifuensis; breeding: selecting myzus persicae, namely the host of aphidius gifuensis, and making the mated aphidius gifuensis to naturally breed with myzus persicae; when myzus persicae start to form mummified aphids, screening and collecting the mummified aphids and storing the mummified aphids in a storage bottle to obtain the bred mummified aphids. The breeding method mates and cultivates the artificially bred aphidius gifuensis and the field aphidius gifuensis, improves the eclosion rate and the spawning rate of aphidius gifuensis, meanwhile improves the activity of aphidius gifuensis and the quality of the aphidius gifuensis species, and prevents the aphidius gifuensis species from degrading.
Description
Technical field
The present invention relates to biological technical field, in particular to
a kind of mating system preventing aphidius gifuensis population deterioration.
Background technology
Aphid is one of destructive insect of most on the earth, it endangers serious for agriculture and forestry and horticulture, often be clustered in the positions such as blade, tender stem, bud, terminal bud, food water is sucked by thorn, make leaf-shrinkage, curling, lopsided, cause the mechanical damage of plant nutrition loss and organ, in addition, aphid can propagate viral diseases of plants, the honeydew of its secretion can grow the causal organisms such as various plants fungus and bacterium, cause the Occurrence & epidemic of plant disease, aphid has extremely strong instinct of reproduction in addition simultaneously.For controlling aphid to the persecution of plant, through repeatedly attempting showing that aphidius gifuensis is the parasitic enemy insect of the special parasitic aphid of a class, very remarkable to the natural contral effect of aphid, effectively can carry out prevention and control to cigarette aphid, to this, for obtaining large batch of aphidius gifuensis, people carry out anti-Tobacco Control aphid by artificial feeding aphidius gifuensis mostly, the method of this breeding aphidius gifuensis in enormous quantities achieves the good control effect of aphid, artificial breeding aphidius gifuensis anti-Tobacco Control aphid technology is utilized can effectively to reduce using of chemical pesticide, improve the safety of tobacco leaf and all the other agricultural product, available protecting environment.
But, there is following defect in artificial breeding aphidius gifuensis: artificial propagation aphidius gifuensis is carrying out for a long time breeding under the condition that close relative breeds substantially, this mode of reproduction often makes the eclosion rate of aphidius gifuensis, spawning rate, life-span, activity waits degradation, thus cause the aphidius gifuensis population quality degradation of artificial breeding, aphidius gifuensis Quality Down reduces causing aphidius gifuensis the control effect of cigarette aphid, therefore how to prevent artificial breeding aphidius gifuensis population deterioration from becoming current vital research topic, this by indirect relation to the process of aphidius gifuensis to cigarette aphid Control Technology, even be related to the safety of tobacco leaf and all the other agricultural product.
Summary of the invention
The degradation such as eclosion rate, spawning rate, life-span, activity of aphidius gifuensis is often caused for solving artificial breeding aphidius gifuensis in prior art, thus cause the aphidius gifuensis population quality degradation of artificial breeding, and then cause aphidius gifuensis to defects such as the control effect reductions of cigarette aphid, the object of the present invention is to provide
a kind of mating system preventing aphidius gifuensis population deterioration.
For achieving the above object, provide in the embodiment of the present invention
a kind of mating system preventing aphidius gifuensis population deterioration, this mating system comprises the following steps:
S101, honeybee kind gather: gather the stiff aphid of artificial breeding aphidius gifuensis formation and the stiff aphid of field aphidius gifuensis formation respectively, and be stored in respectively in test tube by the stiff aphid gathered;
S102, honeybee kind are cultivated: be placed in climatic cabinate by test tube and cultivate, after in vitro stiff aphid sprouts wings into peak, purify to invisible spectro aphidius gifuensis;
S103, the mating of honeybee kind: the aphidius gifuensis of in vitro artificial breeding and field aphidius gifuensis are released to and samely in vitro carry out mating;
S104, to breed: the host's cigarette aphid selecting aphidius gifuensis, and the aphidius gifuensis of post-coitum and host's cigarette aphid are placed on breed Indoor Natural and breed;
S105, screen stiff aphid and preserve: breeding indoor host's cigarette aphid when starting to form stiff aphid, screening and gather stiff aphid and be placed in storage bottle and preserve, namely obtaining the stiff aphid after breeding.
Mating system provided by the invention is simple, convenient operation, aphidius gifuensis provenance and field aphidius gifuensis provenance by screening high-quality artificial breeding in the technical program ensure that quality and the activity of provenance, the aphidius gifuensis provenance of artificial breeding and field aphidius gifuensis Interspecific mate, post-coitum carries out cultivating and breeding, solve that prior art is medium-term and long-term carries out close relative and breed and make aphidius gifuensis population occur the active problem such as poor, mating system provided by the invention improves the eclosion rate of aphidius gifuensis population, the quality of spawning rate and population, prevent aphidius gifuensis population deterioration, due to kind of source quality and activity higher, so ensure that the activity of the aphidius gifuensis after breeding is all higher, improve the quality of the aphidius gifuensis after breeding, and then improve aphidius gifuensis to cigarette aphid control effect, practical, be convenient to promote.
Further, in step S101, the step that the stiff aphid formed the aphidius gifuensis of artificial breeding gathers is:
1. when the aphidius gifuensis of artificial breeding forms stiff aphid, select the stiff aphid individual large, glossiness is good, screening in every 3-6 month is a collection of, and often criticizing artificial breeding aphidius gifuensis total amount with the ratio often criticizing aphidius gifuensis collection capacity is 1:0.33 × 10
-4-0.67 × 10
-4;
2. the stiff aphid after screening is collected in vitro, and uses nylon wire to cover test tube mouth.
In the technical program, in order to ensure the quality of provenance, aphidius gifuensis for artificial breeding should select the stiff aphid that individuality is large, glossiness is good, ensure that the health degree of the stiff aphid of provenance, to obtain screening in every 3-6 month by great many of experiments a collection of in the present invention in addition, and often criticizing artificial breeding aphidius gifuensis total amount with the ratio often criticizing aphidius gifuensis collection capacity is 1:0.33 × 10
-4-0.67 × 10
-4data area can screen the stiff aphid obtaining better quality, if lower than these data, reduce screening effeciency, extend the time of breeding, poor practicability, if improve the collection capacity often criticized, then cannot ensure the quality of provenance.
In this technical scheme, often criticizing artificial breeding aphidius gifuensis total amount with the ratio often criticizing aphidius gifuensis collection capacity is 1:0.33 × 10
-4-0.67 × 10
-4data area obtain according to aphidius gifuensis deterioration law.
Further, in step S101, to the step that the stiff aphid of field aphidius gifuensis formation gathers be:
1. at ecological bar
part is good, field acquisition field aphidius gifuensis that Pesticide use is few, when field aphidius gifuensis forms stiff aphid, select individual large, stiff aphid that glossiness is good, the ratio of the collection capacity of artificial breeding aphidius gifuensis collection capacity and field aphidius gifuensis is 1:0.3-0.5;
2. the stiff aphid after screening is collected in vitro, and uses nylon wire to cover test tube mouth.
In the technical program, test tube is convenient to the collection of the stiff aphid of aphidius gifuensis; Test tube can stop the impact of the stiff aphids of factor pair aphidius gifuensis such as the mushroom that is harmful to aphidius gifuensis or its natural enemy; Nylon wire covers test tube mouth can ensure that aphidius gifuensis grows normally; Nylon wire covers the factors such as test tube mouth can prevent in vitro humidity excessive and causes the hypogenetic factor of aphidius gifuensis; Nylon wire covers test tube mouth can ensure that the temp. and humidity that next step processes is synchronous.
Field aphidius gifuensis Individual Size differs, and in order to ensure the quality of provenance, needing screening and gathering individual large, the good stiff aphid of glossiness, and ensureing that the ratio of the collection capacity of artificial breeding aphidius gifuensis collection capacity and field aphidius gifuensis is 1:0.3-0.5.
Further, in step S102, in vitro stiff aphid cultivation, the step of purifying are: be placed in the climatic cabinate that cultivation temperature is 20-25 DEG C, humidity is 50-80% by the test tube of the stiff aphid of band and cultivate, after invisible spectro stiff aphid sprouts wings into peak, reject hyperparasitism honeybee and weak, that body is residual aphidius gifuensis, namely complete purification.
In the technical program, cultivate under suitable environment after the aphidius gifuensis of artificial breeding and field aphidius gifuensis collection, in incubation, reject the quality that hyperparasitism honeybee and weak, that body is residual aphidius gifuensis are mainly used in improving aphidius gifuensis provenance.
In addition, also define temperature and pressure parameter in the technical program, this temperature and humidity is the optimum condition that aphidius gifuensis grows, simultaneously the synchronism that can be sprouted wings by aphidius gifuensis of this condition, for aphidius gifuensis physical efficiency is supplemented and mating lay a good foundation.
Further, in step S102, after invisible spectro aphidius gifuensis is purified, give invisible spectro aphidius gifuensis respectively and carry out physical efficiency supply, the method of physical efficiency supply is: the cotton swab speckling with white sand syrup is placed in the test tube 2-3 hour with aphidius gifuensis, carries out physical efficiency supply to aphidius gifuensis.Physical efficiency supply be mainly used in for aphidius gifuensis cultivation and breed physical efficiency support be provided, ensure that aphidius gifuensis can normally be bred and grow, what improve aphidius gifuensis breeds speed.
Further, in step S103, the step that the aphidius gifuensis of artificial breeding and field aphidius gifuensis carry out mating is:
1. the aphidius gifuensis of artificial breeding and the aphidius gifuensis in field are released in vitro same, the female ratio of the hero of in vitro aphidius gifuensis is kept to be 0.5-1:1, when the female ratio of hero of in vitro aphidius gifuensis is greater than 1, remove the part Xiong Feng of in vitro individual little, poor activity;
2. above-mentioned test tube is placed on that temperature is 12-20 DEG C, humidity is 50-80%, intensity of illumination is natural mating 8-12 hour in the environment of 1, namely completes the aphidius gifuensis of artificial breeding and the mating of field aphidius gifuensis.
In the technical program, further describe the concrete steps that the aphidius gifuensis of artificial breeding and field aphidius gifuensis carry out mating, the female ratio of the hero of in vitro aphidius gifuensis should be kept in mating process to be 0.5-1, prove by experiment, this hero is female can realize higher mating efficiency than scope, if when male female ratio is greater than 1 in addition, remove in vitro individual little, the part Xiong Feng of poor activity, the mating quality of principal security aphidius gifuensis, in addition, temperature, the parameter restriction principal security of humidity parameter keeps activity and the life-span of post-coitum aphidius gifuensis under normal mating, temperature or the too high aphidius gifuensis physical stamina letdown of humidity, lifetime, temperature, the activity of the too low aphidius gifuensis of humidity is too poor is unfavorable for mating.
Further, in step S103, when the aphidius gifuensis of artificial breeding and field aphidius gifuensis carry out post-coitum, the aphidius gifuensis giving post-coitum carries out physical efficiency supply, the method of physical efficiency supply is: the test tube 1-2 hour cotton swab speckling with white sand syrup being placed in the aphidius gifuensis with post-coitum, and test tube is placed on temperature is 12-20 DEG C, humidity is 50-80%, intensity of illumination is carry out physical efficiency supply in the environment of 0.In the physical efficiency supply in this stage, mainly for the aphidius gifuensis of post-coitum provides physical efficiency support, ensure that the aphidius gifuensis of post-coitum can normally be bred and grow.
The cigarette aphid that further, in step S104, host's cigarette aphid should select to grow fast, physical health, growth is 2-4 age.Host's cigarette aphid is mainly used in as aphidius gifuensis provides host, in order to ensure that aphidius gifuensis can normally be bred, needs to select to grow parasitic cigarette aphid that is fast, physical health.Grow, kind that the cigarette aphid of physical health can ensure aphidius gifuensis and grow high-quality nutriment required and prevent and treat cigarette aphid disease in spite of illness simultaneously and bring in aphidius gifuensis body, the cigarette aphid in 2-4 age is the worm age of aphidius gifuensis hobby and main parasitic cigarette aphid, the cigarette aphid parasitic rate of aphidius gifuensis to this period of worm age is the highest, and the cigarette aphid in this worm age is beneficial to growing of aphidius gifuensis simultaneously.
Further, in step S104, the aphidius gifuensis of post-coitum and the step of host's cigarette aphid natural breed are: aphidius gifuensis and parasitic cigarette aphid are released to the indoor of breeding that temperature is 15-25 DEG C, humidity is 50-80% simultaneously and carry out natural breed, breed the honeybee aphid of indoor release than being 1:50-100.This temperature, damp condition scope are wide, be convenient to control simultaneously, aphidius gifuensis can be good at growing under this temperature, damp condition simultaneously, honeybee aphid ratio mainly on average can be laid eggs about 200 according to an aphidius gifuensis, simultaneously in order to the uniformity of the stiff aphid formation time of satisfied raising, if ratio is crossed high energy consumption and also easily wasted greatly, the too low aphidius gifuensis of ratio reduces the stiff aphid uniformity of formation aphidius gifuensis simultaneously to the parasitic rate of cigarette aphid and also reduces.
Further, breed indoor host's cigarette aphid when starting to form stiff aphid, to the step that stiff aphid is screened and stores be: screen by stages and gather individual larger stiff aphid and be placed in storage bottle, and storage bottle to be placed in temperature be preserve under the condition of 5-7 DEG C.In the technical program, ensure that the stiff aphid individuality of the aphidius gifuensis of storage is large, best in quality, improve the worm uniformity in age that ensure that the stiff aphid of stored aphidius gifuensis simultaneously, in addition, this storage condition aphidius gifuensis grows slowly, the memory cycle is long, can ensure the quality storing rear aphidius gifuensis simultaneously.
Beneficial effect of the present invention is: the present invention is by using the aphidius gifuensis of artificial breeding and field aphidius gifuensis mating and carrying out eclosion rate and the spawning rate of culture and improvement aphidius gifuensis, improve the activity of aphidius gifuensis simultaneously, improve the quality of aphidius gifuensis population, prevent aphidius gifuensis population deterioration.
Embodiment
Below by specific embodiment, the present invention is described in further detail.
Described in embodiment one, the embodiment of the present invention
a kind of mating system preventing aphidius gifuensis population deterioration, this mating system comprises the following steps:
S101, honeybee kind gather: gather the stiff aphid of artificial breeding aphidius gifuensis formation and the stiff aphid of field aphidius gifuensis formation respectively, and be stored in respectively in test tube by the stiff aphid gathered;
The step that further the described stiff aphid formed the aphidius gifuensis of artificial breeding gathers is: 1. when the aphidius gifuensis of artificial breeding forms stiff aphid, select the stiff aphid that individuality is large, glossiness is good, screening in every 3 months is a collection of, and often criticizing artificial breeding aphidius gifuensis total amount with the ratio often criticizing aphidius gifuensis collection capacity is 1:0.33 × 10
-4if be in this embodiment the aphidius gifuensis kind often criticizing 3,000 ten thousand artificial breedings, need screening 1000; 2. the stiff aphid after screening is collected in vitro, and uses nylon wire to cover test tube mouth.
The step that further the described stiff aphid formed field aphidius gifuensis gathers is: 1. at ecological bar
part is good, field acquisition field aphidius gifuensis that Pesticide use is few, when field aphidius gifuensis forms stiff aphid, select the stiff aphid that individuality is large, glossiness is good, the ratio of the collection capacity of artificial breeding aphidius gifuensis collection capacity and field aphidius gifuensis is 1:0.3, in this embodiment, owing to having screened the aphidius gifuensis of 1000 artificial breedings, then field aphidius gifuensis has needed screening 300; 2. the stiff aphid after screening is collected in vitro, and uses nylon wire to cover test tube mouth.
S102, honeybee kind are cultivated: be placed in climatic cabinate by test tube and cultivate, after in vitro stiff aphid sprouts wings into peak, purify to invisible spectro aphidius gifuensis;
The step that further described in vitro stiff aphid is cultivated, purify is: the test tube of the stiff aphid of band is placed in cultivation temperature is 20 DEG C, humidity be 50% climatic cabinate in cultivate, after invisible spectro stiff aphid sprouts wings into peak, reject hyperparasitism honeybee and weak, that body is residual aphidius gifuensis, namely complete purification.
Further described after invisible spectro aphidius gifuensis is purified, give invisible spectro aphidius gifuensis respectively and carry out physical efficiency supply, the method of physical efficiency supply is: the cotton swab speckling with white sand syrup is placed in the test tube 2 hours with aphidius gifuensis, carries out physical efficiency supply to aphidius gifuensis.
S103, the mating of honeybee kind: the aphidius gifuensis of in vitro artificial breeding and field aphidius gifuensis are released to and samely in vitro carry out mating;
The step that the aphidius gifuensis of artificial breeding described further and field aphidius gifuensis carry out mating is: be 1. released in vitro same by the aphidius gifuensis of artificial breeding and the aphidius gifuensis in field, the female ratio of the hero of in vitro aphidius gifuensis is kept to be 0.5:1, when the female ratio of hero of in vitro aphidius gifuensis is greater than 1, remove the part Xiong Feng of in vitro individual little, poor activity; 2. above-mentioned test tube is placed on that temperature is 12 DEG C, humidity is 50%, intensity of illumination is natural mating 8 hours in the environment of 1, namely complete the aphidius gifuensis of artificial breeding and the mating of field aphidius gifuensis.
The aphidius gifuensis when artificial breeding described further and field aphidius gifuensis carry out post-coitum, the aphidius gifuensis giving post-coitum carries out physical efficiency supply, the method of physical efficiency supply is: the cotton swab speckling with white sand syrup is placed in the test tube 1 hour with the aphidius gifuensis of post-coitum, and test tube is placed on temperature is 12 DEG C, humidity is 50%, intensity of illumination is carry out physical efficiency supply in the environment of 0.
S104, to breed: the host's cigarette aphid selecting aphidius gifuensis, and the aphidius gifuensis of post-coitum and host's cigarette aphid are placed on breed Indoor Natural and breed;
The cigarette aphid that further described host's cigarette aphid should select to grow fast, physical health, growth was 2 ages.
The aphidius gifuensis of post-coitum described further and the step of host's cigarette aphid natural breed are: aphidius gifuensis and cigarette aphid are released to the indoor of breeding that temperature is 15 DEG C, humidity is 50% simultaneously and carry out natural breed, breed the honeybee aphid of indoor release than being 1:50.
S105, screen stiff aphid and preserve: breeding indoor host's cigarette aphid when starting to form stiff aphid, screening and gather stiff aphid and be placed in storage bottle and preserve, namely obtaining the stiff aphid after breeding.
Further describedly breed indoor host's cigarette aphid when starting to form stiff aphid, to the step that stiff aphid is screened and stores be: screen by stages and gather individual larger stiff aphid and be placed in storage bottle, and storage bottle to be placed in temperature be preserve under the condition of 5 DEG C.
Experiment one: use the aphidius gifuensis of artificial breeding merely in the aphidius gifuensis that obtains of above-described embodiment one and prior art to carry out breeding and recording data testing Tanaka, it is bred and carries out physical examinations to two kinds of aphidius gifuensis respectively afterwards, obtains following data:
From above-mentioned data, with in prior art merely by compared with artificial breeding aphidius gifuensis, by mating system provided by the invention, improve quality and the health degree of aphidius gifuensis, improve eclosion rate and the spawning rate of aphidius gifuensis, improve the activity of aphidius gifuensis simultaneously, the strong adaptability of the aphidius gifuensis using mating system provided by the invention to breed out, improve the quality of aphidius gifuensis population, prevent aphidius gifuensis population deterioration.
Described in embodiment two, the embodiment of the present invention
a kind of mating system preventing aphidius gifuensis population deterioration, this mating system comprises the following steps:
S101, honeybee kind gather: gather the stiff aphid of artificial breeding aphidius gifuensis formation and the stiff aphid of field aphidius gifuensis formation respectively, and be stored in respectively in test tube by the stiff aphid gathered;
The step that further the described stiff aphid formed the aphidius gifuensis of artificial breeding gathers is: 1. when the aphidius gifuensis of artificial breeding forms stiff aphid, select the stiff aphid that individuality is large, glossiness is good, screening in every 5 months is a collection of, and often criticizing artificial breeding aphidius gifuensis total amount with the ratio often criticizing aphidius gifuensis collection capacity is 1:0.50 × 10
-4if be in this embodiment the aphidius gifuensis kind often criticizing 3,000 ten thousand artificial breedings, need screening 1500; 2. the stiff aphid after screening is collected in vitro, and uses nylon wire to cover test tube mouth.
The step that further the described stiff aphid formed field aphidius gifuensis gathers is: 1. at ecological bar
part is good, field acquisition field aphidius gifuensis that Pesticide use is few, when field aphidius gifuensis forms stiff aphid, select the stiff aphid that individuality is large, glossiness is good, the ratio of the collection capacity of artificial breeding aphidius gifuensis collection capacity and field aphidius gifuensis is 1:0.4, in this embodiment, owing to having screened the aphidius gifuensis of 1500 artificial breedings, then field aphidius gifuensis has needed screening 600; 2. the stiff aphid after screening is collected in vitro, and uses nylon wire to cover test tube mouth.
S102, honeybee kind are cultivated: be placed in climatic cabinate by test tube and cultivate, after in vitro stiff aphid sprouts wings into peak, purify to invisible spectro aphidius gifuensis;
The step that further described in vitro stiff aphid is cultivated, purify is: the test tube of the stiff aphid of band is placed in cultivation temperature is 23 DEG C, humidity be 65% climatic cabinate in cultivate, after invisible spectro stiff aphid sprouts wings into peak, reject hyperparasitism honeybee and weak, that body is residual aphidius gifuensis, namely complete purification.
Further described after invisible spectro aphidius gifuensis is purified, give invisible spectro aphidius gifuensis respectively and carry out physical efficiency supply, the method of physical efficiency supply is: the cotton swab speckling with white sand syrup is placed in the test tube 2.5 hours with aphidius gifuensis, carries out physical efficiency supply to aphidius gifuensis.
S103, the mating of honeybee kind: the aphidius gifuensis of in vitro artificial breeding and field aphidius gifuensis are released to and samely in vitro carry out mating;
The step that the aphidius gifuensis of artificial breeding described further and field aphidius gifuensis carry out mating is: be 1. released in vitro same by the aphidius gifuensis of artificial breeding and the aphidius gifuensis in field, the female ratio of the hero of in vitro aphidius gifuensis is kept to be 0.8:1, when the female ratio of hero of in vitro aphidius gifuensis is greater than 1, remove the part Xiong Feng of in vitro individual little, poor activity; 2. above-mentioned test tube is placed on that temperature is 16 DEG C, humidity is 65%, intensity of illumination is natural mating 10 hours in the environment of 1, namely complete the aphidius gifuensis of artificial breeding and the mating of field aphidius gifuensis.
The aphidius gifuensis when artificial breeding described further and field aphidius gifuensis carry out post-coitum, the aphidius gifuensis giving post-coitum carries out physical efficiency supply, the method of physical efficiency supply is: the cotton swab speckling with white sand syrup is placed in the test tube 1.5 hours with the aphidius gifuensis of post-coitum, and test tube is placed on temperature is 16 DEG C, humidity is 65%, intensity of illumination is carry out physical efficiency supply in the environment of 0.
S104, to breed: the host's cigarette aphid selecting aphidius gifuensis, and the aphidius gifuensis of post-coitum and host's cigarette aphid are placed on breed Indoor Natural and breed;
The cigarette aphid that further described host's cigarette aphid should select to grow fast, physical health, growth was 3 ages.
The aphidius gifuensis of post-coitum described further and the step of host's cigarette aphid natural breed are: aphidius gifuensis and parasitic cigarette aphid are released to the indoor of breeding that temperature is 20 DEG C, humidity is 65% simultaneously and carry out natural breed, breed the honeybee aphid of indoor release than being 1:75.
S105, screen stiff aphid and preserve: breeding indoor host's cigarette aphid when starting to form stiff aphid, screening and gather stiff aphid and be placed in storage bottle and preserve, namely obtaining the stiff aphid after breeding.
Further describedly breed indoor host's cigarette aphid when starting to form stiff aphid, to the step that stiff aphid is screened and stores be: screen by stages and gather individual larger stiff aphid and be placed in storage bottle, and storage bottle to be placed in temperature be preserve under the condition of 6 DEG C.
Experiment two: use the aphidius gifuensis of artificial breeding merely in the aphidius gifuensis that obtains of above-described embodiment one and prior art to carry out breeding and recording data testing Tanaka, it is bred and carries out physical examinations to two kinds of aphidius gifuensis respectively afterwards, obtains following data:
From above-mentioned data, with in prior art merely by compared with artificial breeding aphidius gifuensis, by mating system provided by the invention, improve quality and the health degree of aphidius gifuensis, improve eclosion rate and the spawning rate of aphidius gifuensis, improve the activity of aphidius gifuensis simultaneously, the strong adaptability of the aphidius gifuensis using mating system provided by the invention to breed out, improve the quality of aphidius gifuensis population, prevent aphidius gifuensis population deterioration.
A kind of mating system preventing aphidius gifuensis population deterioration described in embodiment three, the embodiment of the present invention, this mating system comprises the following steps:
S101, honeybee kind gather: gather the stiff aphid of artificial breeding aphidius gifuensis formation and the stiff aphid of field aphidius gifuensis formation respectively, and be stored in respectively in test tube by the stiff aphid gathered;
The step that further the described stiff aphid formed the aphidius gifuensis of artificial breeding gathers is: 1. when the aphidius gifuensis of artificial breeding forms stiff aphid, select the stiff aphid that individuality is large, glossiness is good, screening in every 3-6 month is a collection of, and often criticizing artificial breeding aphidius gifuensis total amount with the ratio often criticizing aphidius gifuensis collection capacity is 1:0.67 × 10
-4, in this embodiment, screen 2000 by the aphidius gifuensis kind of 3,000 ten thousand artificial breedings; 2. the stiff aphid after screening is collected in vitro, and uses nylon wire to cover test tube mouth.
The step that further the described stiff aphid formed field aphidius gifuensis gathers is: 1. at ecological bar
part is good, field acquisition field aphidius gifuensis that Pesticide use is few, when field aphidius gifuensis forms stiff aphid, select the stiff aphid that individuality is large, glossiness is good, the ratio of the collection capacity of artificial breeding aphidius gifuensis collection capacity and field aphidius gifuensis is 1:0.5, in this embodiment, owing to having screened the aphidius gifuensis of 2000 artificial breedings, then field aphidius gifuensis has needed screening 1000; 2. the stiff aphid after screening is collected in vitro, and uses nylon wire to cover test tube mouth.
S102, honeybee kind are cultivated: be all placed in climatic cabinate by test tube and cultivate, after in vitro stiff aphid sprouts wings into peak, purify to invisible spectro aphidius gifuensis;
The step that further described in vitro stiff aphid is cultivated, purify is: the test tube of the stiff aphid of band is placed in cultivation temperature is 25 DEG C, humidity be 80% climatic cabinate in cultivate, after invisible spectro stiff aphid sprouts wings into peak, reject hyperparasitism honeybee and weak, that body is residual aphidius gifuensis, namely complete purification.
Further described after invisible spectro aphidius gifuensis is purified, give invisible spectro aphidius gifuensis respectively and carry out physical efficiency supply, the method of physical efficiency supply is: the cotton swab speckling with white sand syrup is placed in the test tube 3 hours with aphidius gifuensis, carries out physical efficiency supply to aphidius gifuensis.
S103, the mating of honeybee kind: the aphidius gifuensis of in vitro artificial breeding and field aphidius gifuensis are released to and samely in vitro carry out mating;
The step that the aphidius gifuensis of artificial breeding described further and field aphidius gifuensis carry out mating is: be 1. released in vitro same by the aphidius gifuensis of artificial breeding and the aphidius gifuensis in field, the female ratio of the hero of in vitro aphidius gifuensis is kept to be 1:1, when the female ratio of hero of in vitro aphidius gifuensis is greater than 1, remove the part Xiong Feng of in vitro individual little, poor activity; 2. above-mentioned test tube is placed on that temperature is 20 DEG C, humidity is 80%, intensity of illumination is natural mating 12 hours in the environment of 1, namely complete the aphidius gifuensis of artificial breeding and the mating of field aphidius gifuensis.
The aphidius gifuensis when artificial breeding described further and field aphidius gifuensis carry out post-coitum, the aphidius gifuensis giving post-coitum carries out physical efficiency supply, the method of physical efficiency supply is: the cotton swab speckling with white sand syrup is placed in the test tube 2 hours with the aphidius gifuensis of post-coitum, and test tube is placed on temperature is 20 DEG C, humidity is 80%, intensity of illumination is carry out physical efficiency supply in the environment of 0.
S104, to breed: the host's cigarette aphid selecting aphidius gifuensis, and the aphidius gifuensis of post-coitum and host's cigarette aphid are placed on breed Indoor Natural and breed;
The cigarette aphid that further described host's cigarette aphid should select to grow fast, physical health, growth was 4 ages.
The aphidius gifuensis of post-coitum described further and the step of host's cigarette aphid natural breed are: aphidius gifuensis and parasitic cigarette aphid are released to the indoor of breeding that temperature is 25 DEG C, humidity is 80% simultaneously and carry out natural breed, breed the honeybee aphid of indoor release than being 1:100.
S105, screen stiff aphid and preserve: breeding indoor host's cigarette aphid when starting to form stiff aphid, screening and gather stiff aphid and be placed in storage bottle and preserve, namely obtaining the stiff aphid after breeding.
Further describedly breed indoor host's cigarette aphid when starting to form stiff aphid, to the step that stiff aphid is screened and stores be: screen by stages and gather individual larger stiff aphid and be placed in storage bottle, and storage bottle to be placed in temperature be preserve under the condition of 7 DEG C.
Experiment three: use the aphidius gifuensis of artificial breeding merely in the aphidius gifuensis that obtains of above-described embodiment one and prior art to carry out breeding and recording data testing Tanaka, it is bred and carries out physical examinations to two kinds of aphidius gifuensis respectively afterwards, obtains following data:
From above-mentioned data, with in prior art merely by compared with artificial breeding aphidius gifuensis, by mating system provided by the invention, improve quality and the health degree of aphidius gifuensis, improve eclosion rate and the spawning rate of aphidius gifuensis, improve the activity of aphidius gifuensis simultaneously, the strong adaptability of the aphidius gifuensis using mating system provided by the invention to breed out, improve the quality of aphidius gifuensis population, prevent aphidius gifuensis population deterioration.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for a person skilled in the art, the present invention can have various modifications and variations.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (10)
1. prevent a mating system for aphidius gifuensis population deterioration, it is characterized in that, this mating system comprises the following steps:
S101, honeybee kind gather: gather the stiff aphid of artificial breeding aphidius gifuensis formation and the stiff aphid of field aphidius gifuensis formation respectively, and be stored in respectively in test tube by the stiff aphid gathered;
S102, honeybee kind are cultivated: be placed in climatic cabinate by test tube and cultivate, after in vitro stiff aphid sprouts wings into peak, purify to invisible spectro aphidius gifuensis;
S103, the mating of honeybee kind: the aphidius gifuensis of in vitro artificial breeding and field aphidius gifuensis are released to and samely in vitro carry out mating;
S104, to breed: the host's cigarette aphid selecting aphidius gifuensis, and the aphidius gifuensis of post-coitum and host's cigarette aphid are placed on breed Indoor Natural and breed;
S105, screen stiff aphid and preserve: breeding indoor host's cigarette aphid when starting to form stiff aphid, screening and gather stiff aphid and be placed in storage bottle and preserve, namely obtaining the stiff aphid after breeding.
2. the mating system preventing aphidius gifuensis population deterioration according to claim 1, is characterized in that, in step S101, the step that the stiff aphid formed the aphidius gifuensis of artificial breeding gathers is:
1. when the aphidius gifuensis of artificial breeding forms stiff aphid, select the stiff aphid individual large, glossiness is good, screening in every 3-6 month is a collection of, and often criticizing artificial breeding aphidius gifuensis total amount with the ratio often criticizing aphidius gifuensis collection capacity is 1:0.33 × 10
-4-0.67 × 10
-4;
2. the stiff aphid after screening is collected in vitro, and uses nylon wire to cover test tube mouth.
3. the mating system preventing aphidius gifuensis population deterioration according to claim 2, is characterized in that, in step S101, to the step that the stiff aphid of field aphidius gifuensis formation gathers is:
1., field acquisition field aphidius gifuensis that Pesticide use few good at ecological condition, when field aphidius gifuensis forms stiff aphid, select the stiff aphid that individuality is large, glossiness is good, the ratio of the collection capacity of artificial breeding aphidius gifuensis collection capacity and field aphidius gifuensis is 1:0.3-0.5;
2. the stiff aphid after screening is collected in vitro, and uses nylon wire to cover test tube mouth.
4. the mating system preventing aphidius gifuensis population deterioration according to claim 1, it is characterized in that, in step S102, in vitro stiff aphid cultivation, the step of purifying are: be placed in the climatic cabinate that cultivation temperature is 20-25 DEG C, humidity is 50-80% by the test tube of the stiff aphid of band and cultivate, after invisible spectro stiff aphid sprouts wings into peak, reject hyperparasitism honeybee and weak, that body is residual aphidius gifuensis, namely complete purification.
5. the mating system preventing aphidius gifuensis population deterioration according to claim 1, it is characterized in that, in step S102, after invisible spectro aphidius gifuensis is purified, give invisible spectro aphidius gifuensis respectively and carry out physical efficiency supply, the method of physical efficiency supply is: the cotton swab speckling with white sand syrup is placed in the test tube 2-3 hour with aphidius gifuensis, carries out physical efficiency supply to aphidius gifuensis.
6. the mating system preventing aphidius gifuensis population deterioration according to claim 1, is characterized in that, in step S103, the step that the aphidius gifuensis of artificial breeding and field aphidius gifuensis carry out mating is:
1. the aphidius gifuensis of artificial breeding and the aphidius gifuensis in field are released in vitro same, the female ratio of the hero of in vitro aphidius gifuensis is kept to be 0.5-1:1, when the female ratio of hero of in vitro aphidius gifuensis is greater than 1, remove the part Xiong Feng of in vitro individual little, poor activity;
2. above-mentioned test tube is placed on that temperature is 12-20 DEG C, humidity is 50-80%, intensity of illumination is natural mating 8-12 hour in the environment of 1, namely completes the aphidius gifuensis of artificial breeding and the mating of field aphidius gifuensis.
7. the mating system preventing aphidius gifuensis population deterioration according to claim 1, it is characterized in that, in step S103, when the aphidius gifuensis of artificial breeding and field aphidius gifuensis carry out post-coitum, the aphidius gifuensis giving post-coitum carries out physical efficiency supply, the method of physical efficiency supply is: the test tube 1-2 hour cotton swab speckling with white sand syrup being placed in the aphidius gifuensis with post-coitum, and test tube is placed on temperature is 12-20 DEG C, humidity is 50-80%, intensity of illumination is carry out physical efficiency supply in the environment of 0.
8. the mating system preventing aphidius gifuensis population deterioration according to claim 1, is characterized in that, the cigarette aphid that in step S104, host's cigarette aphid should select to grow fast, physical health, growth is 2-4 age.
9. the mating system preventing aphidius gifuensis population deterioration according to claim 1, it is characterized in that, in step S104, the aphidius gifuensis of post-coitum and the step of host's cigarette aphid natural breed are: aphidius gifuensis and parasitic cigarette aphid are released to the indoor of breeding that temperature is 15-25 DEG C, humidity is 50-80% simultaneously and carry out natural breed, breed the honeybee aphid of indoor release than being 1:50-100.
10. the mating system preventing aphidius gifuensis population deterioration according to claim 1, it is characterized in that, in step S105, breed indoor host's cigarette aphid when starting to form stiff aphid, to the step that stiff aphid is screened and stores be: screen by stages and gather individual larger stiff aphid and be placed in storage bottle, and storage bottle to be placed in temperature be preserve under the condition of 5-7 DEG C.
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