CN106259208A - A kind of tobacco plant repeatedly breeds the method for aphidius gifuensis - Google Patents
A kind of tobacco plant repeatedly breeds the method for aphidius gifuensis Download PDFInfo
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- CN106259208A CN106259208A CN201610641438.7A CN201610641438A CN106259208A CN 106259208 A CN106259208 A CN 106259208A CN 201610641438 A CN201610641438 A CN 201610641438A CN 106259208 A CN106259208 A CN 106259208A
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- aphid
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- strain
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- 235000002637 Nicotiana tabacum Nutrition 0.000 title claims abstract description 33
- 241001305034 Aphidius gifuensis Species 0.000 title claims abstract description 30
- 244000061176 Nicotiana tabacum Species 0.000 title claims abstract description 30
- 238000000034 method Methods 0.000 title claims abstract description 21
- 241001124076 Aphididae Species 0.000 claims abstract description 153
- 241000256844 Apis mellifera Species 0.000 claims abstract description 119
- 235000019504 cigarettes Nutrition 0.000 claims abstract description 92
- 241000196324 Embryophyta Species 0.000 claims abstract description 13
- 238000009395 breeding Methods 0.000 claims description 31
- 230000001488 breeding effect Effects 0.000 claims description 31
- 241000208125 Nicotiana Species 0.000 claims 6
- 235000001674 Agaricus brunnescens Nutrition 0.000 abstract description 2
- 241000238631 Hexapoda Species 0.000 abstract description 2
- 230000002265 prevention Effects 0.000 description 7
- 230000000694 effects Effects 0.000 description 4
- 241000700605 Viruses Species 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 2
- 230000013011 mating Effects 0.000 description 2
- 244000045947 parasite Species 0.000 description 2
- 230000007115 recruitment Effects 0.000 description 2
- 230000033458 reproduction Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- UUTKICFRNVKFRG-WDSKDSINSA-N (4R)-3-[oxo-[(2S)-5-oxo-2-pyrrolidinyl]methyl]-4-thiazolidinecarboxylic acid Chemical compound OC(=O)[C@@H]1CSCN1C(=O)[C@H]1NC(=O)CC1 UUTKICFRNVKFRG-WDSKDSINSA-N 0.000 description 1
- 241001600407 Aphis <genus> Species 0.000 description 1
- 241001425390 Aphis fabae Species 0.000 description 1
- 235000015001 Cucumis melo var inodorus Nutrition 0.000 description 1
- 240000002495 Cucumis melo var. inodorus Species 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241000819999 Nymphes Species 0.000 description 1
- 241000180219 Sitobion avenae Species 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000034303 cell budding Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 230000027272 reproductive process Effects 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Botany (AREA)
- Cultivation Of Plants (AREA)
- Catching Or Destruction (AREA)
Abstract
The invention belongs to the technical field of biological control of agriculture and forestry insect, be specifically related to a kind of method that tobacco plant repeatedly breeds aphidius gifuensis, cultivate including cigarette aphid host plant, the most numerous honeybee, the most numerous honeybee, the most numerous honeybee, the 4th numerous honeybee, make full use of the root system that cigarette strain is flourishing, promote that young leaves is sprouted and mushrooms out, make cigarette strain be reused, it is not necessary to carry out secondary nursery and transplanting, saving of work and time, greatly improves the efficiency that aphidius gifuensis is bred.
Description
Technical field
The invention belongs to the technical field of biological control of agriculture and forestry insect, be specifically related to a kind of tobacco plant and repeatedly breed cigarette
The method of aphid parasite.
Background technology
Aphid is one of primary pest during farm-forestry crop produces, and in leaf tobacco production, the harm of aphid is particularly acute, main
On the Nicotiana tabacum L. back of the body and tender stem, to suck Nicotiana tabacum L. juice with adult and nymph, to cause cigarette strain to grow short and small, nutritional imbalance, affect cigarette
The normal growth of strain is grown, and the honeydew of aphid secretion easily induces the generation of the diseases such as dark mildew, and aphid is also propagated multiple simultaneously
Tobacco virus, causes the popular of tobacco virus；Mainly have chemical prevention and physical control to aphid, chemical prevention relies on agriculture
Aphid eliminated by medicine, and bio-diversity can be caused to reduce, and destroys ecological environment, and pollution of agricultural products affects food safety, resisting of aphid
The property of medicine strengthens, and prevention effect declines, and physical control relies primarily on yellow plate prevention and control alatae worm, when alatae worm grows surely on plant
Time, yellow plate has just scattered and disappeared the prevention and control effect to aphid.
In Agro-ecology development, biological prevention is anti-one of the most effective approach that eliminates aphis, aphidius gifuensis
Being the advantage parasite of cigarette aphid, all have distribution in China various places, aphidius gifuensis takes food juice in ovum originates in aphid body and kills
Dead or control aphid, breed the offspring of oneself, it has good prevention effect to cigarette aphid, simultaneously to grain aphid, wheat y-bend
The aphids such as aphid, bean aphid, cotten aphid also have certain control effect, and at present, aphidius gifuensis artificial breeding technology, host plant is most
For Nicotiana tabacum L., having two kinds of mating systems: seedling breeding bee method and strain breeding bee method, their breeding cycle is oversize, and existing this
Both of which can only be bred once, will breed second time and must re-start nursery and transplant cigarette strain, be badly in need of research a kind of steady
The mating system of the fixed aphidius gifuensis efficiently shortening numerous honeybee cycle.
Summary of the invention
For the deficiencies in the prior art, the present invention provides a kind of stability and high efficiency, it is possible to shorten the breeding cycle of aphidius gifuensis,
Reduce efficient numerous honeybee method of recruitment cost.
The technical scheme is that and be achieved in that: a kind of tobacco plant repeatedly breeds the method for aphidius gifuensis, breed
The step of aphidius gifuensis is as follows:
1) cigarette aphid host plant is cultivated: use floating seedlings method to cultivate the host plant tobacco seedlings of cigarette aphid；
2) the most numerous honeybee:
(1) the numerous aphid of aphid is connect: starting to connect aphid when the tobacco seedlings length cultivated in step 1) is to 4 to 5 true leaves, every strain tobacco seedlings connects aphid 2 to 3
Head, allows cigarette aphid spread breeding voluntarily；
(2) the numerous honeybee of honeybee is connect: after step (1) connects aphid 5 to 7 days, after i.e. cigarette aphid is evenly distributed and breeds in right amount, by band aphid tobacco seedlings
Being transplanted in numerous honeybee booth, band aphid tobacco seedlings plant/line spacing is 40cm, transplants and is followed by honeybee in 20 to 25 days, and now tobacco seedlings has grown up to cigarette
Strain, can form a large amount of stiff aphid and aphidius gifuensis for after connecing honeybee 15 to 20 days；
3) the most numerous honeybee:
(1) the numerous aphid of aphid is connect: during the most numerous honeybee, cigarette strain root system is undeveloped, is endangered by cigarette aphid simultaneously, cigarette strain and cigarette strain blade
All without too big, when numerous honeybee initially forms a large amount of stiff aphid for the first time, the harm of cigarette aphid gradually alleviates, and cigarette strain root system is the most gradually sent out
Reaching, cigarette strain top begins to send out young leaves, now by being covered with the blade removal of stiff aphid, leaves and takes the young leaves on top, due to well developed root system,
The speed of growth of young leaves quickly, connects aphid on young leaves, and every strain cigarette strain connects aphid 20 to 30, allows cigarette aphid spread breeding voluntarily；
(2) the numerous honeybee of honeybee is connect: after connecing aphid 10 to 15 days in step (1), i.e. cigarette aphid is evenly distributed and amount reproduction is followed by honeybee, connects honeybee
A large amount of stiff aphid and aphidius gifuensis within latter 15 to 20 days, can be formed；
4) the most numerous honeybee:
(1) the numerous aphid of aphid is connect: the most numerous honeybee is terminated rear cigarette strain root system and still locates the flourishing phase, and cigarette strain top still can send young leaves,
Now by being covered with the blade removal of stiff aphid, leave and take the young leaves on top, due to well developed root system, the speed of growth of young leaves quickly, newly
Connecing aphid on leaf, every strain cigarette strain connects aphid 20 to 30, allows cigarette aphid spread breeding voluntarily；
(2) connecing the numerous honeybee of honeybee: after connecing aphid 10 to 15 days in step (1), i.e. cigarette aphid is evenly distributed and breeding is followed by honeybee in right amount, connects honeybee
A large amount of stiff aphid and aphidius gifuensis within latter 15 to 20 days, can be formed；
5) the 4th numerous honeybee:
(1) the numerous aphid of aphid is connect: it is higher or buddingged that the most numerous honeybee terminates rear cigarette strain, will not send young leaves again, but root system is still
The most flourishing, in this case cigarette strain can be pinched or chop off from middle part, and leave and take two blades, promote sprouting and the life of axillalry bud
Long, treat that axillalry bud blade grows, two blade removals will left and taken when pinching, axillalry bud blade connects aphid, each axillalry bud connects aphid 10
To 15, cigarette aphid is allowed to spread breeding voluntarily；
(2) connecing the numerous honeybee of honeybee: after connecing aphid 10 to 15 days in step (1), i.e. cigarette aphid is evenly distributed and breeding is followed by honeybee in right amount, connects honeybee
A large amount of stiff aphid and aphidius gifuensis within latter 15 to 20 days, can be formed.
Described numerous honeybee canopy temperature is 17 to 27 degrees Celsius, and humidity is 30% to 70%.
When numerous honeybee of described first time, the most numerous honeybee, the most numerous honeybee and the 4th numerous honeybee connect honeybee, honeybee aphid ratio is 1:
50 to 1:100.
The present invention has following beneficial effect: cigarette strain can recycle, and utilizes the cigarette strain after growing up to carry out the most numerous honeybee,
During the most numerous honeybee, using band aphid to transplant, the repoductive time of cigarette aphid and the growth time of cigarette strain are put together, reduce host as far as possible
The cultivation time of plant, shorten numerous honeybee cycle greatly；Stability and high efficiency, it is not necessary to the cultivation repeatedly of host plant, reduces recruitment
Cost, saving of work and time.
Detailed description of the invention
A kind of tobacco plant repeatedly breeds the method for aphidius gifuensis, and the step breeding aphidius gifuensis is as follows: 1) cigarette aphid host
Plant cultivation: use floating seedlings method to cultivate the host plant tobacco seedlings of cigarette aphid；2) the most numerous honeybee: (1) connects the numerous aphid of aphid: treat step
1) starting to connect aphid when the tobacco seedlings length cultivated in is to 4 to 5 true leaves, every strain tobacco seedlings connects aphid 2 to 3, allows cigarette aphid spread breeding voluntarily；
(2) connect the numerous honeybee of honeybee: after step (1) connects aphid 5 to 7 days, after i.e. cigarette aphid is evenly distributed and breeds in right amount, band aphid tobacco seedlings is transplanted
In numerous honeybee booth, band aphid tobacco seedlings plant/line spacing is 40cm, transplants and is followed by honeybee in 20 to 25 days, and now tobacco seedlings has grown up to cigarette strain,
A large amount of stiff aphid and aphidius gifuensis within after connecing honeybee 15 to 20 days, can be formed；3) the most numerous honeybee: (1) connects the numerous aphid of aphid: the most numerous honeybee
Time cigarette strain root system undeveloped, endangered by cigarette aphid simultaneously, cigarette strain and cigarette strain blade are all without too big, and when for the first time, numerous honeybee starts
When forming a large amount of stiff aphid, the harm of cigarette aphid gradually alleviates, and cigarette strain root system is the most flourishing, and cigarette strain top begins to send out young leaves, this
Time by being covered with the blade removal of stiff aphid, leave and take the young leaves on top, due to well developed root system, the speed of growth of young leaves quickly, at young leaves
On connect aphid, every strain cigarette strain connects aphid 20 to 30, allows cigarette aphid spread breeding voluntarily；(2) the numerous honeybee of honeybee is connect: treat step (1) connects aphid 10
After 15 days, i.e. cigarette aphid is evenly distributed and amount reproduction is followed by honeybee, within after connecing honeybee 15 to 20 days, can form a large amount of stiff aphid and cigarette aphid
Cocoon honeybee；4) the most numerous honeybee: (1) connects the numerous aphid of aphid: the most numerous honeybee is terminated rear cigarette strain root system and still locates the flourishing phase, and cigarette strain top is still
Young leaves can be sent, now by being covered with the blade removal of stiff aphid, leave and take the young leaves on top, due to well developed root system, the growth of young leaves
Speed quickly, connects aphid on young leaves, and every strain cigarette strain connects aphid 20 to 30, allows cigarette aphid spread breeding voluntarily；(2) the numerous honeybee of honeybee is connect: treat
After connecing aphid 10 to 15 days in step (1), i.e. cigarette aphid is evenly distributed and breeding is followed by honeybee in right amount, within after connecing honeybee 15 to 20 days, can be formed
A large amount of stiff aphids and aphidius gifuensis；5) the 4th numerous honeybee: (1) connects the numerous aphid of aphid: it is higher or that the most numerous honeybee terminates rear cigarette strain
Budding, young leaves will not be sent again, but root system is the most flourishing, in this case cigarette strain can be pinched or chop off from middle part, and leave and take
Two blades, promote sprouting and the growth of axillalry bud, treat that axillalry bud blade grows, two blade removals will left and taken when pinching, at axil
Connecing aphid on bud-leaf sheet, each axillalry bud connects aphid 10 to 15, allows cigarette aphid spread breeding voluntarily；(2) the numerous honeybee of honeybee is connect: treat in step (1)
After connecing aphid 10 to 15 days, i.e. cigarette aphid is evenly distributed and breeding is followed by honeybee in right amount, within after connecing honeybee 15 to 20 days, can form a large amount of stiff aphid
And aphidius gifuensis.Described numerous honeybee canopy temperature is 17 to 27 degrees Celsius, and humidity is 30% to 70%.Described first time is numerous
When honeybee, the most numerous honeybee, the most numerous honeybee and the 4th numerous honeybee connect honeybee, honeybee aphid ratio is 1:50 to 1:100.
The method using Nicotiana tabacum L. to breed aphidius gifuensis as host plant in prior art has: seedling breeding bee method and strain are numerous
Honeybee method.Seedling breeding bee method is the method using floating seedlings, started to connect aphid the most after planting 50 to 60 days tobacco seedlings grand cross phase and enters
Cigarette aphid breeds row, starts to connect honeybee after within 10 to 15 days, cigarette aphid is formed in a large number, within 15 to 20 days, can form a large amount of stiff aphid and become honeybee, whole
Breeding cycle was at about 80 to 90 days.Strain breeding bee method is to start for after planting 70 to 80 days when tobacco seedlings length to 5 to 6 true leaves
Transplant, within 25 to 30 days, connect aphid after transplanting when cigarette strain length is to 9 to 12 true leaves, after 15 to 20 days, introduce into honeybee, connect honeybee 15 to
Within 20 days, can produce a large amount of stiff aphid and become honeybee, breeding cycle is more than 120 days.
The growth with host plant of breeding of host in aphidius gifuensis reproductive process is placed on same stage and carries out by the present invention,
Greatly shorten numerous honeybee cycle, shorten 45 to 85 days, meanwhile, make full use of the root system that cigarette strain is flourishing, promote that young leaves is sprouted also
Mushroom out, make cigarette strain be reused, it is not necessary to carry out secondary nursery and transplanting, saving of work and time, greatly improve cigarette aphid
The efficiency that cocoon honeybee breeds.
Claims (3)
1. the method that a tobacco plant repeatedly breeds aphidius gifuensis, it is characterised in that the step breeding aphidius gifuensis is as follows:
1) cigarette aphid host plant is cultivated: use floating seedlings method to cultivate the host plant tobacco seedlings of cigarette aphid；
2) the most numerous honeybee:
(1) the numerous aphid of aphid is connect: starting to connect aphid when the tobacco seedlings length cultivated in step 1) is to 4 to 5 true leaves, every strain tobacco seedlings connects aphid 2 to 3
Head, allows cigarette aphid spread breeding voluntarily；
(2) the numerous honeybee of honeybee is connect: after step (1) connects aphid 5 to 7 days, after i.e. cigarette aphid is evenly distributed and breeds in right amount, by band aphid tobacco seedlings
Being transplanted in numerous honeybee booth, band aphid tobacco seedlings plant/line spacing is 40cm, transplants and is followed by honeybee in 20 to 25 days, and now tobacco seedlings has grown up to cigarette
Strain, can form a large amount of stiff aphid and aphidius gifuensis for after connecing honeybee 15 to 20 days；
3) the most numerous honeybee:
(1) the numerous aphid of aphid is connect: during the most numerous honeybee, cigarette strain root system is undeveloped, is endangered by cigarette aphid simultaneously, cigarette strain and cigarette strain blade
All without too big, when numerous honeybee initially forms a large amount of stiff aphid for the first time, the harm of cigarette aphid gradually alleviates, and cigarette strain root system is the most gradually sent out
Reaching, cigarette strain top begins to send out young leaves, now by being covered with the blade removal of stiff aphid, leaves and takes the young leaves on top, due to well developed root system,
The speed of growth of young leaves quickly, connects aphid on young leaves, and every strain cigarette strain connects aphid 20 to 30, allows cigarette aphid spread breeding voluntarily；
(2) the numerous honeybee of honeybee is connect: after connecing aphid 10 to 15 days in step (1), i.e. cigarette aphid is evenly distributed and amount reproduction is followed by honeybee, connects honeybee
A large amount of stiff aphid and aphidius gifuensis within latter 15 to 20 days, can be formed；
4) the most numerous honeybee:
(1) the numerous aphid of aphid is connect: the most numerous honeybee is terminated rear cigarette strain root system and still locates the flourishing phase, and cigarette strain top still can send young leaves,
Now by being covered with the blade removal of stiff aphid, leave and take the young leaves on top, due to well developed root system, the speed of growth of young leaves quickly, newly
Connecing aphid on leaf, every strain cigarette strain connects aphid 20 to 30, allows cigarette aphid spread breeding voluntarily；
(2) connecing the numerous honeybee of honeybee: after connecing aphid 10 to 15 days in step (1), i.e. cigarette aphid is evenly distributed and breeding is followed by honeybee in right amount, connects honeybee
A large amount of stiff aphid and aphidius gifuensis within latter 15 to 20 days, can be formed；
5) the 4th numerous honeybee:
(1) the numerous aphid of aphid is connect: it is higher or buddingged that the most numerous honeybee terminates rear cigarette strain, will not send young leaves again, but root system is still
The most flourishing, in this case cigarette strain can be pinched or chop off from middle part, and leave and take two blades, promote sprouting and the life of axillalry bud
Long, treat that axillalry bud blade grows, two blade removals will left and taken when pinching, axillalry bud blade connects aphid, each axillalry bud connects aphid 10
To 15, cigarette aphid is allowed to spread breeding voluntarily；
(2) connecing the numerous honeybee of honeybee: after connecing aphid 10 to 15 days in step (1), i.e. cigarette aphid is evenly distributed and breeding is followed by honeybee in right amount, connects honeybee
A large amount of stiff aphid and aphidius gifuensis within latter 15 to 20 days, can be formed.
Tobacco plant the most according to claim 1 repeatedly breeds the method for aphidius gifuensis, it is characterised in that described numerous honeybee
Canopy temperature is 17 to 27 degrees Celsius, and humidity is 30% to 70%.
Tobacco plant the most according to claim 1 repeatedly breeds the method for aphidius gifuensis, it is characterised in that described first
When secondary numerous honeybee, the most numerous honeybee, the most numerous honeybee and the 4th numerous honeybee connect honeybee, honeybee aphid ratio is 1:50 to 1:100.
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| CN201610641438.7A CN106259208B (en) | 2016-08-08 | 2016-08-08 | A kind of method that tobacco plant repeatedly breeds aphidius gifuensis |
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| CN201610641438.7A CN106259208B (en) | 2016-08-08 | 2016-08-08 | A kind of method that tobacco plant repeatedly breeds aphidius gifuensis |
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| CN106259208B CN106259208B (en) | 2019-07-05 |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107372372A (en) * | 2017-09-08 | 2017-11-24 | 云南省烟草公司红河州公司 | A kind of method for regenerating cigarette strain breeding cigarette aphid and aphidius gifuensis |
| CN107371764A (en) * | 2017-09-08 | 2017-11-24 | 云南省烟草公司楚雄州公司 | A kind of method that wet tobacco seedlings breeding cigarette aphid and aphidius gifuensis are floated using regeneration |
| CN107455341A (en) * | 2017-08-01 | 2017-12-12 | 中国烟草总公司福建省公司 | A kind of aphidius gifuensis mating system of regulation and control host crop growth |
| CN109832229A (en) * | 2019-02-28 | 2019-06-04 | 山东省果树研究所 | A kind of device and application method detecting the remote diffusivity of entomopathogenic nematode |
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| CN105165313A (en) * | 2015-07-24 | 2015-12-23 | 中国烟草总公司四川省公司 | Method for breeding aphidius gifuensis within short cycle |
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| CN101664017A (en) * | 2009-09-30 | 2010-03-10 | 云南省烟草农业科学研究院 | Method for propagating sulzer braconid flies |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107455341A (en) * | 2017-08-01 | 2017-12-12 | 中国烟草总公司福建省公司 | A kind of aphidius gifuensis mating system of regulation and control host crop growth |
| CN107372372A (en) * | 2017-09-08 | 2017-11-24 | 云南省烟草公司红河州公司 | A kind of method for regenerating cigarette strain breeding cigarette aphid and aphidius gifuensis |
| CN107371764A (en) * | 2017-09-08 | 2017-11-24 | 云南省烟草公司楚雄州公司 | A kind of method that wet tobacco seedlings breeding cigarette aphid and aphidius gifuensis are floated using regeneration |
| CN107372372B (en) * | 2017-09-08 | 2020-09-08 | 云南省烟草公司红河州公司 | Method for reproducing myzus persicae and aphidius gifuensis by regenerating tobacco plants |
| CN109832229A (en) * | 2019-02-28 | 2019-06-04 | 山东省果树研究所 | A kind of device and application method detecting the remote diffusivity of entomopathogenic nematode |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106259208B (en) | 2019-07-05 |
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