CN106172248A - A kind of expanding propagation method of Japan food a red-spotted lizard aphid chalcid fly - Google Patents
A kind of expanding propagation method of Japan food a red-spotted lizard aphid chalcid fly Download PDFInfo
- Publication number
- CN106172248A CN106172248A CN201610584035.3A CN201610584035A CN106172248A CN 106172248 A CN106172248 A CN 106172248A CN 201610584035 A CN201610584035 A CN 201610584035A CN 106172248 A CN106172248 A CN 106172248A
- Authority
- CN
- China
- Prior art keywords
- red
- japan
- spotted lizard
- food
- chalcid fly
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000270322 Lepidosauria Species 0.000 title claims abstract description 62
- 241000317929 Chalcididae Species 0.000 title claims abstract description 59
- 241001124076 Aphididae Species 0.000 title claims abstract description 57
- 235000013305 food Nutrition 0.000 title claims abstract description 52
- 238000000034 method Methods 0.000 title claims abstract description 44
- 238000005065 mining Methods 0.000 claims abstract description 70
- 230000003071 parasitic effect Effects 0.000 claims abstract description 48
- 230000008569 process Effects 0.000 claims abstract description 18
- 238000011081 inoculation Methods 0.000 claims abstract description 16
- 230000003716 rejuvenation Effects 0.000 claims abstract description 7
- 241000256844 Apis mellifera Species 0.000 claims description 49
- 241000819999 Nymphes Species 0.000 claims description 48
- 241000196324 Embryophyta Species 0.000 claims description 22
- 229920001971 elastomer Polymers 0.000 claims description 21
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 15
- 235000015001 Cucumis melo var inodorus Nutrition 0.000 claims description 14
- 240000002495 Cucumis melo var. inodorus Species 0.000 claims description 14
- 238000004659 sterilization and disinfection Methods 0.000 claims description 14
- 239000007788 liquid Substances 0.000 claims description 13
- 230000003203 everyday effect Effects 0.000 claims description 11
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 10
- 229930006000 Sucrose Natural products 0.000 claims description 10
- 239000005720 sucrose Substances 0.000 claims description 10
- 241000382353 Pupa Species 0.000 claims description 9
- 241001465977 Coccoidea Species 0.000 claims description 8
- 239000005842 Thiophanate-methyl Substances 0.000 claims description 8
- QGHREAKMXXNCOA-UHFFFAOYSA-N thiophanate-methyl Chemical group COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC QGHREAKMXXNCOA-UHFFFAOYSA-N 0.000 claims description 8
- 238000003756 stirring Methods 0.000 claims description 7
- 238000012258 culturing Methods 0.000 claims description 6
- 230000028327 secretion Effects 0.000 claims description 6
- 239000004563 wettable powder Substances 0.000 claims description 6
- 239000013589 supplement Substances 0.000 claims description 4
- 230000004083 survival effect Effects 0.000 abstract description 10
- 241000607479 Yersinia pestis Species 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 4
- 230000001488 breeding effect Effects 0.000 description 11
- 238000009395 breeding Methods 0.000 description 10
- 244000043261 Hevea brasiliensis Species 0.000 description 9
- 238000011161 development Methods 0.000 description 8
- 230000018109 developmental process Effects 0.000 description 8
- 230000012010 growth Effects 0.000 description 7
- 230000017448 oviposition Effects 0.000 description 6
- 230000001954 sterilising effect Effects 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 238000010998 test method Methods 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 4
- 230000024241 parasitism Effects 0.000 description 4
- 230000029264 phototaxis Effects 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 229920000742 Cotton Polymers 0.000 description 3
- 241000219112 Cucumis Species 0.000 description 3
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 3
- 241000258937 Hemiptera Species 0.000 description 3
- 241000238631 Hexapoda Species 0.000 description 3
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 230000002265 prevention Effects 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 238000010923 batch production Methods 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 238000004140 cleaning Methods 0.000 description 2
- MCWXGJITAZMZEV-UHFFFAOYSA-N dimethoate Chemical compound CNC(=O)CSP(=S)(OC)OC MCWXGJITAZMZEV-UHFFFAOYSA-N 0.000 description 2
- 235000013601 eggs Nutrition 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000011010 flushing procedure Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 230000012447 hatching Effects 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- TVLSRXXIMLFWEO-UHFFFAOYSA-N prochloraz Chemical compound C1=CN=CN1C(=O)N(CCC)CCOC1=C(Cl)C=C(Cl)C=C1Cl TVLSRXXIMLFWEO-UHFFFAOYSA-N 0.000 description 2
- 230000001850 reproductive effect Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 241001547764 Aphelinidae Species 0.000 description 1
- 241000256837 Apidae Species 0.000 description 1
- 241000256848 Apis florea Species 0.000 description 1
- 206010003694 Atrophy Diseases 0.000 description 1
- 241001450758 Ceroplastes Species 0.000 description 1
- 241000813617 Ceroplastes floridensis Species 0.000 description 1
- 241001450756 Ceroplastes rubens Species 0.000 description 1
- 241000207199 Citrus Species 0.000 description 1
- 241001415288 Coccidae Species 0.000 description 1
- 241001547770 Coccophagus Species 0.000 description 1
- 241001074651 Coccophagus ceroplastae Species 0.000 description 1
- 102000002322 Egg Proteins Human genes 0.000 description 1
- 108010000912 Egg Proteins Proteins 0.000 description 1
- 241001147399 Encyrtidae Species 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 206010053759 Growth retardation Diseases 0.000 description 1
- 241000257303 Hymenoptera Species 0.000 description 1
- 206010023126 Jaundice Diseases 0.000 description 1
- 241001093152 Mangifera Species 0.000 description 1
- IHYABEGFQYPORI-UHFFFAOYSA-N Nymphone Natural products COC1=C(OC)C(OC)=CC(C(=O)C2C(C(OC2)C=2C=C3OCOC3=CC=2)CO)=C1 IHYABEGFQYPORI-UHFFFAOYSA-N 0.000 description 1
- 241000309680 Parasaissetia Species 0.000 description 1
- 241000452638 Parasaissetia nigra Species 0.000 description 1
- 229910000746 Structural steel Inorganic materials 0.000 description 1
- 241001481304 Vespoidea Species 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000037444 atrophy Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 235000020971 citrus fruits Nutrition 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- -1 dult Species 0.000 description 1
- 239000000686 essence Substances 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 231100000001 growth retardation Toxicity 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 230000000266 injurious effect Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- MEBQXILRKZHVCX-UHFFFAOYSA-N methidathion Chemical compound COC1=NN(CSP(=S)(OC)OC)C(=O)S1 MEBQXILRKZHVCX-UHFFFAOYSA-N 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004899 motility Effects 0.000 description 1
- 229920003052 natural elastomer Polymers 0.000 description 1
- 229920001194 natural rubber Polymers 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 210000004681 ovum Anatomy 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 238000004080 punching Methods 0.000 description 1
- 230000027272 reproductive process Effects 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000009469 supplementation Effects 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- YFNCATAIYKQPOO-UHFFFAOYSA-N thiophanate Chemical compound CCOC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OCC YFNCATAIYKQPOO-UHFFFAOYSA-N 0.000 description 1
- 231100000167 toxic agent Toxicity 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses the expanding propagation method of a kind of Japan food a red-spotted lizard aphid chalcid fly, comprise the following steps: the expanding propagation of expanding propagation (3) the Japan food a red-spotted lizard aphid chalcid fly of process (2) the vector roadway caving mining of (1) host plant and rejuvenation.The present invention has collection and the inoculation method of vector simple, efficient, improve the survival rate of vector roadway caving mining, maximize the utilization rate improving host plant, improve Japan's food a red-spotted lizard aphid chalcid fly parasitic rate to vector, achieve the control to pest population quantity, ensure field control effect.
Description
Technical field
The present invention relates to agriculture and forestry injurious insect technical field of biological control, particularly relate to the expanding propagation side of a kind of Japan food a red-spotted lizard aphid chalcid fly
Method.
Background technology
Roadway caving mining (Parasaissetia nigra Nitner) has another name called rubber helmet a red-spotted lizard, pitch-black secondary helmet a red-spotted lizard, belongs to same wing
Mesh Hemiptera, Coccoidea Coccoidea, wax coccidae Coccidae, secondary pearl lecanium belongs to Parasaissetia.This worm exists in recent years
On rubber tree, large area outburst is caused disaster, and has had a strong impact on production and the development of China's natural rubber.
Roadway caving mining host is various, has more than 160 kind of host plant of 36 sections according to records, be rubber, Maninot esculenta crantz., Sirikaya,
The important pests of the tropical crops such as Citrus, coffee, Cotton Gossypii, Fructus psidii guajavae immaturus, rubber Fructus Mangifera Indicae, Fructus Chaenomelis.This worm mainly inhales with adult, nymph thorn
The blade of host plant and the juice of twig, cause branch and leaf jaundice, atrophy, fallen leaves, branch to dry up；It addition, this worm also secretes
Honeydew induction dark mildew, affects the photosynthesis of plant.When causing harm light, host plant growth retardation and yield is caused to decline；Sternly
During weight, the whole strain of plant is withered, and according to statistics, this worm can cause the loss of rubber more than 11%.
At present, the preventing and treating to roadway caving mining the most still utilizes chemical agent, but along with to this preferable oxygen of worm preventive effect
Change Rogor, the disabling of the contour toxicant of methidathion, and poisoning the limiting the use of of medicament such as Rogor, chlopyrifos, its medicament range of choice
Narrower, rubber tree height is big in addition, it is difficult to medicament is delivered to the position that is injured of gum, thus causes its prevention effect more
Undesirable.It addition, the use destructible bio-diversity of high-toxic pesticide, kill a large amount of pest natural enemy, it is difficult to insect is carried out
Continue prevention and control.
Utilizing natural enemy to carry out biological prevention and control is the important technology preventing and treating scale insect, utilizes parasitic wasp to prevent and treat scale insect both at home and abroad
Also there are many successfully examples.Roadway caving mining is on rubber after population outbreak, and it has been carried out natural enemy money by domestic many units
The investigation in source, research on utilization, filtered out Japanese food a red-spotted lizard aphid chalcid fly, Coccophagus ceroplastae, secondary pearl lecanium wealthy handle encyrtid, excellent
The field advantage parasitic wasps such as refined qi pulse chalcid fly.
Japan food a red-spotted lizard aphid chalcid fly Cocophagus japonicus Compere belongs to Hymenoptera Hymenoptera, aphid chalcid fly
Section Aphelinidae, Coccophagus Coccophagus, be a kind of widely distributed lecanium parasitic wasp, Chinese, Japanese, beautiful
State all has been reported that, host has ceroplastes floridensis, ceroplastes rubens, ceroplastes, brown soft wax a red-spotted lizard, Japan's lecanium, oil lam black helmet kuwana, pseudo-ceroplastes etc.
Multiple lecanium.Japan food a red-spotted lizard aphid chalcid fly can parasitic roadway caving mining 2 age nymph, 3 age nymph, initial stage adult, brown phase adult,
After parasitic roadway caving mining, can sprout wings in its every coccid body and a parasitic wasp.Through investigation, this honeybee is distributed widely in rubber
Young growth and open and cut woods, is a kind of parasite with applications well prospect.While it is true, owing to this honeybee is at the nature of woodland
Population quantity is the highest, and insect does not reaches the purpose of natural contral.If able to realize artificial breeding, then at roadway caving mining
2 age nymph, 3 age nymph, initial stage adult, brown phase adult time discharge in a large number, then can improve the parasitic rate to roadway caving mining, real
The now control to pest population quantity.For the research of this honeybee, mainly collect in terms of host, distribution, form both at home and abroad, have no people
Work breeding utilizes, and the data also having no relevant is available for using for reference.Therefore, in order to realize the effective utilization to Japan food a red-spotted lizard aphid chalcid fly,
Need to provide a kind of low cost, simple and easy to do, the scale that is applicable to numerous honeybee method.
Summary of the invention
In view of this, the invention provides a kind of Japan food a red-spotted lizard aphid chalcid fly expanding propagation method, solve reproductive efficiency low, become
This height and the inefficient problem of parasitism.
The technological means that the present invention uses is as follows: the expanding propagation method of a kind of Japan food a red-spotted lizard aphid chalcid fly, comprises the following steps:
(1) process of host plant
Select Fructus Cucurbitae moschatae as host plant；Thiophanate methyl wettable powder water is configured to medicinal liquid；To Fructus Cucurbitae moschatae surface
The sterilization processing that carries out disinfection also is dried；
(2) expanding propagation of vector
Select roadway caving mining as vector；Gather the rubber branch with roadway caving mining adult；Put into and only go up
The lucifuge box of surface opening carries out incubating nymph at the beginning of roadway caving mining and collects；Nymph is incubated to Fructus Cucurbitae moschatae surface at the beginning of inoculation roadway caving mining
Cultivate；
(3) expanding propagation of parasitic wasp
Select Japan food a red-spotted lizard aphid chalcid fly as parasitic wasp；The Fructus Cucurbitae moschatae of roadway caving mining nymph step (2) cultivated is put
Carry out parasitic wasp in entering propagation house and connect honeybee；The coccid connecting parasitic wasp is cultivated；The parasitic wasp honeybee kind adding field is entered
Row rejuvenation.
Preferably, described step (2) is cultivated at temperature control indoor inoculation, and temperature is 24～26 DEG C, photoperiod L:D=12h:
12h, humidity 70%～85%.
Preferably, after described step (2) carries out inoculation cultivation, stirring a Fructus Cucurbitae moschatae every day, roadway caving mining was developed to for 2 ages
After nymph, produce more honeydew, rinse secretions honeydew with clear water, until be developed to 3 age nymph or adult.
Preferably, during described step (3) inoculation, the quantitative proportion of parasitic wasp and roadway caving mining nymph is 1:40～60.
Preferably, in described step (3), the temperature of propagation house is 26～28 DEG C, photoperiod L:D=12h:12h, humidity
70%～85%.
Preferably, the Fructus Cucurbitae moschatae inoculating parasitic wasp is taken out by described step (3) cultivation 15～17 days on placement culturing rack.
Preferably, in described step (3) pupa supplement when turning into adult 15～25% sucrose solution carry out expanding propagation.
Preferably, described step (3) adds 30%～50% field Japan food a red-spotted lizard aphid chalcid fly honeybee kind every 2～3 months
Carry out rejuvenation.
Use the expanding propagation method of a kind of Japan provided by the present invention food a red-spotted lizard aphid chalcid fly, have the advantages that
1, the disinfection that the present invention selects thiophanate methyl to carry out host plant Fructus Cucurbitae moschatae processes, and is beneficial to extend making of Fructus Cucurbitae moschatae
Use the time.
2, the present invention utilizes the phototaxis of roadway caving mining, has invented and has incubated nymph at the beginning of roadway caving mining simple, efficient
Collection method and inoculation method.
3, the present invention stirs Fructus Cucurbitae moschatae in connecing for after incubating nymph at the beginning of roadway caving mining one week, makes to incubate at the beginning of roadway caving mining
Each surface of Fructus Cucurbitae moschatae can be evenly distributed on after nymph, maximize the utilization rate improving Fructus Cucurbitae moschatae.
4, invention provides for periodically carrying out the honeydew cleaning in roadway caving mining nymph and adult stage, in being conducive to improving
Between the survival rate of host's roadway caving mining.
5, controlling object roadway caving mining of the present invention is as the vector of the Japan food a red-spotted lizard numerous honeybee of aphid chalcid fly, is conducive to keeping
Japan's food a red-spotted lizard aphid chalcid fly parasitic capacity to roadway caving mining, it is ensured that field control effect.
6, the present invention proposes the expanding propagation conditions such as Japanese optimum temperature, host and the extra-nutrition eating a red-spotted lizard aphid chalcid fly expanding propagation,
Improve the expanding propagation efficiency of Japan food a red-spotted lizard aphid chalcid fly.
7, the present invention can realize anniversary breeding, batch production high-quality Japan food a red-spotted lizard aphid chalcid fly, and can effectively solve breeding effect
Rate is low, Biological control supply and demand problem not in time
Accompanying drawing explanation
Fig. 1 is the schematic flow sheet of the expanding propagation method of a kind of Japan of present invention food a red-spotted lizard aphid chalcid fly.
Detailed description of the invention
Principle and feature to the present invention are described below, and example is served only for explaining the present invention, is not intended to limit
Determine the scope of the present invention.
Embodiment 1
As shown in Figure 1: the expanding propagation method of a kind of Japan food a red-spotted lizard aphid chalcid fly, comprise the following steps:
(1) process of host plant
Select the Fructus Cucurbitae moschatae of 3 jin/complete mature as host plant, clean surface with wet rag, dry standby；By 70%
Thiophanate methyl wettable powder clear water is made into 1000 times of medicinal liquids；Soak medicinal liquid with rag Fructus Cucurbitae moschatae surface to be carried out disinfection sterilization
Process, dry, place 2 days standby.
(2) expanding propagation of vector
Select roadway caving mining as vector；Gather field (with egg-laying season roadway caving mining adult and to have and just incubate
Nymph climbs out of from adult shell) rubber branch；The lucifuge box putting into only upper surface open carries out expanding propagation, on rubber branch
The rubber tree leaf of a few Zhang Xinxian is put in face；Collect and incubate nymph banister bruss at the beginning of roadway caving mining and be swept to Fructus Cucurbitae moschatae surface gently and carry out temperature
Control indoor inoculation is cultivated, and forms the Experimental population with Fructus Cucurbitae moschatae as host.The temperature of temperature controlled compartment is 24 DEG C, photoperiod L:D=12h:
12h, humidity 70%.In one week, every day stirs a Fructus Cucurbitae moschatae so that incubates nymph at the beginning of the roadway caving mining on each surface of Fructus Cucurbitae moschatae and divides
Cloth is uniform.After being developed to 2 age nymphs, its secretions honeydew gradually becomes many, rinses secretions honeydew every 5 days with clear water, then
Make Fructus Cucurbitae moschatae surface rapid airing by electric fan etc., until be developed to 3 age nymph or initial stage adult, a part is used for inoculating parasitism
Honeybee, a part is allowed to continue to grow, and gives over to roadway caving mining breeding kind source.
(3) expanding propagation of parasitic wasp
Select individual Japan big, energetic food a red-spotted lizard aphid chalcid fly as parasitic wasp；The rubber pair pearl wax that step (2) is cultivated
The Fructus Cucurbitae moschatae of a red-spotted lizard nymph or adult is put into the honeybee cage that connects of propagation house and is inoculated parasitic wasp, and the temperature of propagation house is 26 DEG C, the photoperiod
L:D=12h:12h, humidity 70%, parasitic wasp is 1:40 with the quantitative proportion of secondary pearl lecanium nymph, uses hair-dryer after inoculating 2 days
Blow the Japan's food little bee imago of a red-spotted lizard aphid on Fructus Cucurbitae moschatae off；Take out to place by the Fructus Cucurbitae moschatae inoculating parasitic wasp and cultivate 15 days on culturing rack extremely
Pupa, during the fast emerging adult of pupa, the sucrose solution of supplementary 15% moves to connect expanding propagation in honeybee cage；Added in connecing honeybee cage every 2 months
Field 30% parasitic wasp honeybee kind carries out rejuvenation.
Embodiment 2
As shown in Figure 1: the expanding propagation method of a kind of Japan food a red-spotted lizard aphid chalcid fly, comprise the following steps:
(1) process of host plant
The Fructus Cucurbitae moschatae selecting every 5 jin/complete mature cleans surface as the wet rag of host plant, dries standby；Will
70% thiophanate methyl wettable powder clear water is made into 1000 times of medicinal liquids；Soak medicinal liquid with rag Fructus Cucurbitae moschatae surface is carried out disinfection
Sterilization processing, places and dries for 3 days；
(2) expanding propagation of vector
Select roadway caving mining as vector；Gather field (with egg-laying season roadway caving mining adult and to have and just incubate
Nymph climbs out of from adult shell) rubber branch；The lucifuge box putting into only upper surface open carries out expanding propagation, on rubber branch
The rubber tree leaf of a few Zhang Xinxian is put in face；Incubate nymph banister bruss at the beginning of collection roadway caving mining to be swept to gently to Fructus Cucurbitae moschatae surface carry out
Temperature control indoor inoculation is cultivated, and forms the Experimental population with Fructus Cucurbitae moschatae as host.The temperature of temperature controlled compartment is 26 DEG C, photoperiod L:D=
12h:12h, humidity 85%.In one week, every day stirs a Fructus Cucurbitae moschatae so that if incubating at the beginning of the roadway caving mining on each surface of Fructus Cucurbitae moschatae
Worm is evenly distributed.During being developed to 2 age nymphs, rinsed secretions honeydew every 6 days with clear water, then make south by electric fan etc.
Melon surface rapid airing, until be developed to 3 age nymph or initial stage adult, a part is used for inoculating parasitic wasp, and a part is allowed to continue
Supervention is educated, and gives over to roadway caving mining breeding kind source.
(3) expanding propagation of parasitic wasp
Select individual Japan big, energetic food a red-spotted lizard aphid chalcid fly as parasitic wasp；The roadway caving mining that step (2) is cultivated
The Fructus Cucurbitae moschatae of nymph or adult is put into the honeybee cage that connects of propagation house and is inoculated parasitic wasp, and the temperature of propagation house is 28 DEG C, photoperiod L:D=
12h:12h, humidity 85%, parasitic wasp is 1:50 with the quantitative proportion of secondary pearl lecanium nymph, blows south off with hair-dryer after inoculating 3 days
Japan's food little bee imago of a red-spotted lizard aphid on melon；Take out to place by the Fructus Cucurbitae moschatae inoculating parasitic wasp and cultivate 17 days on culturing rack to pupa, pupa
During fast emerging adult, the sucrose solution of supplementary 25% moves to connect expanding propagation in honeybee cage；Field is added at quarterly intervals in connecing honeybee cage
50% parasitic wasp honeybee kind carries out rejuvenation.
Embodiment 3
As shown in Figure 1: the expanding propagation method of a kind of Japan food a red-spotted lizard aphid chalcid fly, comprise the following steps:
(1) process of host plant
The Fructus Cucurbitae moschatae selecting every 4 jin/complete mature cleans surface as the wet rag of host plant, dries standby；Will
70% thiophanate methyl wettable powder clear water is made into 1000 times of medicinal liquids；Soak medicinal liquid with rag Fructus Cucurbitae moschatae surface is carried out disinfection
Sterilization processing, places and dries for 2.5 days；
(2) expanding propagation of vector
Select roadway caving mining as vector；Gather field (with egg-laying season roadway caving mining adult and to have and just incubate
Nymph climbs out of from adult shell) rubber branch；The lucifuge box putting into only upper surface open carries out expanding propagation, on rubber branch
The rubber tree leaf of a few Zhang Xinxian is put in face；Incubate nymph banister bruss at the beginning of collection roadway caving mining to be swept to gently to Fructus Cucurbitae moschatae surface carry out
Temperature control indoor inoculation is cultivated, and forms the Experimental population with Fructus Cucurbitae moschatae as host.The temperature of temperature controlled compartment is 25 DEG C, photoperiod L:D=
12h:12h, humidity 80%.In one week, every day stirs a Fructus Cucurbitae moschatae so that if incubating at the beginning of the roadway caving mining on each surface of Fructus Cucurbitae moschatae
Worm is evenly distributed.During being developed to 2 age nymphs, rinsed secretions honeydew every 5 days with clear water, then make south by electric fan etc.
Melon surface rapid airing, until be developed to 3 age nymph or adult, a part is used for inoculating parasitic wasp, and a part is allowed to continue to send out
Educate, give over to roadway caving mining breeding kind source.
(3) expanding propagation of parasitic wasp
Select individual Japan big, energetic food a red-spotted lizard aphid chalcid fly as parasitic wasp；The roadway caving mining that step (2) is cultivated
The Fructus Cucurbitae moschatae of nymph or adult is put into the honeybee cage that connects of propagation house and is inoculated parasitic wasp, and the temperature of propagation house is 27 DEG C, photoperiod L:D=
12h:12h, humidity 80%, parasitic wasp is 1:60 with the quantitative proportion of secondary pearl lecanium nymph, blows off with hair-dryer after inoculating 2.5 days
The food a red-spotted lizard aphid chalcid fly Japan food little bee imago of a red-spotted lizard aphid on Fructus Cucurbitae moschatae；Take out to place by the Fructus Cucurbitae moschatae inoculating parasitic wasp and cultivate on culturing rack
Within 16 days, to pupa, during the fast emerging adult of pupa, the sucrose solution of supplementary 20% moves to connect expanding propagation in honeybee cage；Every 2.5 months to connecing honeybee
Add field 40% parasitic wasp honeybee kind in cage and carry out rejuvenation.
Wherein, connect honeybee cage: size is the stainless steel frame of long * width * height=100Cm*40Cm*150Cm, centre plank every
Breaking and be divided into 3, every increases illumination system, and end face glass is closed, and the back side and the side gauze of 150 mesh are closed, and front is done
The two semicircle sliding doors opened, seal by gauze clamshell doors, carry out the operation in reproductive process by door.
Culturing rack: size is the angle iron frame of long * width * height=200Cm*40Cm*180Cm, middle plank every point, every floor height
45Cm, installs 40 watts of daylight lamp two on every layer by totally 4 layers.
Sucrose water: the sucrose water of compound concentration about 20% is for the extra-nutrition of Japan's food little bee imago of a red-spotted lizard aphid.
In order to verify beneficial effects of the present invention further, do the checking of following comparative and tested:
1, the selection of host plant
Test method: respectively connect on Fructus Cucurbitae moschatae and rubber just hatching 1 age nymph 200.It is placed in the artificial climate of 25,27 DEG C
Raise in case.Observe, record the development duration of roadway caving mining each age, survival number every day, calculate the survival rate of each age；?
Laying eggs period, if investigating the borer population hatching out every day, and nymph being removed clean, after end of laying eggs, to every rubber pair pearl wax
A red-spotted lizard checks, observes its unhatched ovum grain number, and calculates the parameter and population on two kinds of host plants.Test is repeated 3 times.
Table 1: roadway caving mining life table parameters on Fructus Cucurbitae moschatae and rubber host plant
Table 1 is that roadway caving mining is at rubber and the life table parameters of Fructus Cucurbitae moschatae.Result shows, when Fructus Cucurbitae moschatae is as host plant,
The intrinsic rate of increase of roadway caving mining is the fastest, is best suitable for its growth and breeding.Therefore, each 3～5 jin of heavy one-tenth are selected according to result
Ripe Fructus Cucurbitae moschatae is as the host plant of breeding roadway caving mining.
2, host plant sterilization processing
Test is arranged: by 1000 times of liquid of 10 ripe Fructus Cucurbitae moschatae thiophanate methyls just taken, 1000 liquid of sporgon, many
Bacterium 800 times of liquid of spirit are cleaned, disinfecting surface, are then placed in 26 DEG C of indoor, observe every day 1 time, record the Fructus Cucurbitae moschatae corruption time.Experiment weight
Multiple 3 times.
Several antibacterial of table 2 process after Fructus Cucurbitae moschatae storage time (my god)
Table 2 data are after several antibacterial processes, the holding time of Fructus Cucurbitae moschatae.From table 2, after thiophanate methyl processes,
Under what storage life of Fructus Cucurbitae moschatae, comparison is compared and can be extended about 40 days, and the most considerably longer than sporgon and the record of many bacterium processes.Therefore, with 70%
1000 times of liquid that thiophanate methyl wettable powder is made into are cleaned, sterilization Fructus Cucurbitae moschatae surface, carry out surface disinfection process.
3, the phototaxis test of nymph is incubated at the beginning of roadway caving mining
Experimental technique: only upper surface open keeping away upwards will be put into the rubber tree branch of roadway caving mining adult
Light box, then puts into the rubber tree leaf of a few Zhang Xinxian on branch, and light only enters from the opening part of top box, 3
After hour, on statistics rubber tree leaf and incubate the quantity of nymph in box at the beginning of roadway caving mining elsewhere, calculate light rate.
Result shows: after 3 hours, and the first nymph quantity of incubating on the rubber leaf at light source is 1938, and other direction
For 95, the light rate that becomes is 95.32%.Illustrate that the phototaxis incubating nymph at the beginning of roadway caving mining is obvious.
4, honeydew clearness test
Test method: arrange and rinse Fructus Cucurbitae moschatae surface with clear water after 2 age nymphs and do not clean the process of two kinds of Fructus Cucurbitae moschatae surface, clearly
Quickly dry up with electric fan after washing and naturally dry two kinds of process.When roadway caving mining grows adult, observe statistics survival
Rate.
Result shows: the survival rate 35.4% of non-flushing honeydew, and rushes depositing of periodic flushing Fructus Cucurbitae moschatae surface honeydew with water
Motility rate is 64.2%, and quickly dries up the survival rate that can also improve low instar nymphs after punching with fan.
5, the phase of roadway caving mining is selected by Japan's food a red-spotted lizard aphid chalcid fly
Test method: select on Fructus Cucurbitae moschatae two age nymph, tritonymph, initial stage adult, each 60 of brown phase adult.By ♀
Honeybee: ♂ honeybee: the inoculation proportion of coccid=4:1:60 connects honeybee cover with homemade local and connects honeybee, allows and takes out after chalcid fly parasitism 24h, and go
Except the one-tenth honeybee on Fructus Cucurbitae moschatae, be placed in room temperature and be about 27 DEG C of indoor, every 24h observe 1 time, observe chalcid fly development duration, go out honeybee number,
Property than etc..Experiment is repeated 4 times.
Chalcid fly is grown and the impact of parasitism by table 3 Host Instar
Table 3 data are the different Japan food a red-spotted lizard aphid chalcid fly host age preferences to vector roadway caving mining.Can from table 3
See, Japan's food development duration of a red-spotted lizard aphid chalcid fly and parasitic rate all with roadway caving mining be 3 age nymph the highest, queen bee rate became with the initial stage
Worm is the highest.Therefore, consider roadway caving mining development duration and the parasitic rate of parasitic wasp, go out honeybee number, female than etc., can
Select 3 age nymph～initial stage adult roadway caving mining breeding Japan food a red-spotted lizard aphid chalcid fly.
6, the extra-nutrition in Japan's food a red-spotted lizard aphid chalcid fly adult stage selects
Test method: collect the one-tenth honeybee just sprouted wings, 5 is one to be assembled in test tube (1.5cm × 7.5cm) respectively, the mouth of pipe
Clog with Cotton Gossypii.Select Mel, glucose, fructose, sucrose, be configured to the concentration of 20%, by cotton pellet in above-mentioned sugar liquid
In soak after put in the test tube of dress honeybee and carry out nutritional supplementation, comparison supplements clear water.Often process female 30, put into temperature 27 ± 1
DEG C, relative humidity is 70% ± 10%, in the growth cabinet of photoperiod L:D=12:12, observes the survival becoming honeybee every day 2 times
Situation, calculates Life of Adult.In queen bee: drone: the ratio of coccid=4:1:60 connects honeybee, supplement Mel, the glucose, really of 20%
Sugar, sucrose, compare as blank, connect honeybee and carry out at above-mentioned growth cabinet.The coccid that more renews every day, and dissect and swap out
Take over the coccid of honeybee, count egg laying amount.Experiment is repeated 5 times.
Table 4 data are the Japanese Life of Adult eating a red-spotted lizard aphid chalcid fly and egg laying amount under the conditions of Different Nutrition
Result shows: with sucrose as when supplementing the nutrients, Life of Adult is the longest, and egg laying amount is the highest, can become as during expanding propagation
The extra-nutrition of honeybee.
7, Japan's food a red-spotted lizard aphid chalcid fly expanding propagation temperature selects
Test method: female in 4: 1 is male: the ratio of 60 a red-spotted lizards (3 age) meets honeybee 24h under the conditions of 27 DEG C, enters temperature the most respectively
18,21,24,27,30 DEG C in totally 5 growth cabinets, the development duration of chalcid fly, bee yield, parasitic rate are observed (to grow to pupa
Phase is standard), it is repeated 3 times.Life of Adult: 30 honeybees of each repetition, is repeated 3 times, puts into growth cabinet, observe 1 every day
Secondary chalcid fly survival condition, it was observed that chalcid fly is dead.
The impact on Japan's food a red-spotted lizard aphid chalcid fly population growth of table 5 temperature
Result shows: development duration, with the shortest when 30 DEG C, goes out honeybee number and parasitic rate with the highest when 24 DEG C.Therefore, comprehensively examine
Consider parasitic rate, development duration, go out honeybee number, the factor such as Life of Adult, selects 27 DEG C as the Japanese temperature eating a red-spotted lizard aphid chalcid fly expanding propagation
Condition.
In sum, using the expanding propagation method of a kind of Japan provided by the present invention food a red-spotted lizard aphid chalcid fly, the present invention selects first
Base thiophanate carries out the disinfection of host plant Fructus Cucurbitae moschatae and processes, and is beneficial to extend the use time of Fructus Cucurbitae moschatae.Utilize roadway caving mining
Phototaxis, invented collection method and the inoculation method incubating nymph at the beginning of roadway caving mining simple, efficient.On inoculation rubber pair pearl
Stir Fructus Cucurbitae moschatae in incubating for after nymph one week at the beginning of lecanium, after making to incubate nymph at the beginning of roadway caving mining, each of Fructus Cucurbitae moschatae can be evenly distributed on
Surface, maximizes the utilization rate improving Fructus Cucurbitae moschatae.Define the honeydew cleaning periodically carrying out roadway caving mining nymph and adult stage,
Be conducive to improving the survival rate of vector roadway caving mining.Be conducive to keeping Japan food a red-spotted lizard aphid chalcid fly that roadway caving mining is posted
Raw power, it is ensured that field control effect.Propose the expanding propagation such as Japanese optimum temperature, host and the extra-nutrition eating a red-spotted lizard aphid chalcid fly expanding propagation
Condition, improves the expanding propagation efficiency of Japan food a red-spotted lizard aphid chalcid fly.Can realize anniversary breeding, batch production high-quality Japan food a red-spotted lizard aphid is little
Honeybee, and it is low effectively to solve reproductive efficiency, Biological control supply and demand problem not in time.
The foregoing is only presently preferred embodiments of the present invention, not in order to limit the present invention, all essences in the present invention
Within god and principle, any modification, equivalent substitution and improvement etc. done, within should be included in the scope of protection of the invention.
Claims (8)
1. the expanding propagation method of a Japan food a red-spotted lizard aphid chalcid fly, it is characterised in that comprise the following steps:
(1) process of host plant
Select Fructus Cucurbitae moschatae as host plant；Thiophanate methyl wettable powder water is configured to medicinal liquid；Fructus Cucurbitae moschatae surface is carried out
Disinfection processes and dries；
(2) expanding propagation of vector
Select roadway caving mining as vector；Gather the rubber branch with roadway caving mining adult；Put into only upper surface
The lucifuge box of opening carries out incubating nymph at the beginning of roadway caving mining and collects；Incubate nymph at the beginning of inoculation roadway caving mining to carry out to Fructus Cucurbitae moschatae surface
Cultivate；
(3) expanding propagation of parasitic wasp
Select Japan food a red-spotted lizard aphid chalcid fly as parasitic wasp；The Fructus Cucurbitae moschatae of roadway caving mining nymph step (2) cultivated is put into numerous
Grow indoor to carry out parasitic wasp and connect honeybee；The coccid connecting parasitic wasp is cultivated；The parasitic wasp honeybee kind adding field carries out multiple
Strong.
The expanding propagation method of a kind of Japan the most according to claim 1 food a red-spotted lizard aphid chalcid fly, it is characterised in that described step (2)
Cultivating at temperature control indoor inoculation, temperature is 24～26 DEG C, photoperiod L:D=12h:12h, humidity 70%～85%.
The expanding propagation method of a kind of Japan the most according to claim 1 food a red-spotted lizard aphid chalcid fly, it is characterised in that described step (2)
Carry out after inoculation cultivates, stirring a Fructus Cucurbitae moschatae every day, after roadway caving mining is developed to 2 age nymphs, producing more honeydew, use clear water
Rinse secretions honeydew, until be developed to 3 age nymph or adult.
The expanding propagation method of a kind of Japan the most according to claim 1 food a red-spotted lizard aphid chalcid fly, it is characterised in that described step (3)
During inoculation, parasitic wasp is 1:40～60 with the quantitative proportion of roadway caving mining nymph.
The expanding propagation method of a kind of Japan the most according to claim 1 food a red-spotted lizard aphid chalcid fly, it is characterised in that described step (3)
The temperature of middle propagation house is 26～28 DEG C, photoperiod L:D=12h:12h, humidity 70%～85%.
The expanding propagation method of a kind of Japan the most according to claim 1 food a red-spotted lizard aphid chalcid fly, it is characterised in that described step (3)
Middle taking-up by the Fructus Cucurbitae moschatae inoculating parasitic wasp places cultivation 15～17 days on culturing rack.
The expanding propagation method of a kind of Japan the most according to claim 1 food a red-spotted lizard aphid chalcid fly, it is characterised in that described step (3)
Middle pupa supplement when turning into adult 15～25% sucrose solution carry out expanding propagation.
The expanding propagation method of a kind of Japan the most according to claim 1 food a red-spotted lizard aphid chalcid fly, it is characterised in that described step (3)
In every 2～3 months add 30%～50% field Japan food a red-spotted lizard aphid chalcid fly honeybee kind carry out rejuvenation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610584035.3A CN106172248B (en) | 2016-07-21 | 2016-07-21 | A kind of expanding propagation method of Japan's food a red-spotted lizard aphid chalcid fly |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610584035.3A CN106172248B (en) | 2016-07-21 | 2016-07-21 | A kind of expanding propagation method of Japan's food a red-spotted lizard aphid chalcid fly |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106172248A true CN106172248A (en) | 2016-12-07 |
| CN106172248B CN106172248B (en) | 2017-09-29 |
Family
ID=57492259
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610584035.3A Active CN106172248B (en) | 2016-07-21 | 2016-07-21 | A kind of expanding propagation method of Japan's food a red-spotted lizard aphid chalcid fly |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106172248B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108124822A (en) * | 2017-12-29 | 2018-06-08 | 中国热带农业科学院南亚热带作物研究所 | The method and apparatus for raising the clean mealybug of pineapple |
| CN109526885A (en) * | 2018-12-19 | 2019-03-29 | 中国热带农业科学院环境与植物保护研究所 | A kind of expanding propagation method of Japan's food a red-spotted lizard aphid chalcid fly drone |
| CN113207516A (en) * | 2021-05-31 | 2021-08-06 | 中国热带农业科学院环境与植物保护研究所 | Method for preventing and treating rubber tree lecanium lecanii by using Japanese Lecanicillium lecanii |
| CN113287574A (en) * | 2021-04-30 | 2021-08-24 | 中国热带农业科学院环境与植物保护研究所 | Method for propagating blue Changdou gold wasps |
| CN118140876A (en) * | 2024-04-22 | 2024-06-07 | 中国热带农业科学院环境与植物保护研究所 | Method for breeding mealybug and flea bees by using mealybug |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103168751A (en) * | 2013-04-07 | 2013-06-26 | 中国农业科学院植物保护研究所 | Expanding propagation method for aphidius gifuensis ashmeads |
| CN105104311A (en) * | 2015-09-25 | 2015-12-02 | 黔南州烟草公司瓮安县分公司 | Method for breeding aphidius gifuensis ashmeads through alternanthera philoxeroides |
| CN105165726A (en) * | 2015-05-25 | 2015-12-23 | 中国农业科学院植物保护研究所 | Method for realizing three-dimensional propagation of Aphidius gifuensis Ashmaed by means of cuttage plant |
| CN105165733A (en) * | 2015-09-25 | 2015-12-23 | 黔南州烟草公司瓮安县分公司 | Overwintering breeding method for aphidius gifuensis population |
| CN105211008A (en) * | 2015-11-09 | 2016-01-06 | 云南省烟草公司玉溪市公司 | A kind of method improving dish aphid parasite reproductive efficiency |
| CN105230575A (en) * | 2015-11-09 | 2016-01-13 | 云南省烟草公司玉溪市公司 | Method for increasing lipaphis erysimi breeding efficiency |
-
2016
- 2016-07-21 CN CN201610584035.3A patent/CN106172248B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103168751A (en) * | 2013-04-07 | 2013-06-26 | 中国农业科学院植物保护研究所 | Expanding propagation method for aphidius gifuensis ashmeads |
| CN105165726A (en) * | 2015-05-25 | 2015-12-23 | 中国农业科学院植物保护研究所 | Method for realizing three-dimensional propagation of Aphidius gifuensis Ashmaed by means of cuttage plant |
| CN105104311A (en) * | 2015-09-25 | 2015-12-02 | 黔南州烟草公司瓮安县分公司 | Method for breeding aphidius gifuensis ashmeads through alternanthera philoxeroides |
| CN105165733A (en) * | 2015-09-25 | 2015-12-23 | 黔南州烟草公司瓮安县分公司 | Overwintering breeding method for aphidius gifuensis population |
| CN105211008A (en) * | 2015-11-09 | 2016-01-06 | 云南省烟草公司玉溪市公司 | A kind of method improving dish aphid parasite reproductive efficiency |
| CN105230575A (en) * | 2015-11-09 | 2016-01-13 | 云南省烟草公司玉溪市公司 | Method for increasing lipaphis erysimi breeding efficiency |
Non-Patent Citations (3)
| Title |
|---|
| 张学科等: "食蚧蚜小蜂生物学特性及对寄主的控制力", 《东北林业大学学报》 * |
| 张方平: "斑翅食蚧蚜小蜂生物学及作用效能研究", 《中国优秀硕士学位论文全文数据库》 * |
| 程聪: "橡副珠蜡蚧几种主要天敌昆虫人工扩繁技术研究", 《中国优秀硕士学位论文全文数据库》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108124822A (en) * | 2017-12-29 | 2018-06-08 | 中国热带农业科学院南亚热带作物研究所 | The method and apparatus for raising the clean mealybug of pineapple |
| CN109526885A (en) * | 2018-12-19 | 2019-03-29 | 中国热带农业科学院环境与植物保护研究所 | A kind of expanding propagation method of Japan's food a red-spotted lizard aphid chalcid fly drone |
| CN113287574A (en) * | 2021-04-30 | 2021-08-24 | 中国热带农业科学院环境与植物保护研究所 | Method for propagating blue Changdou gold wasps |
| WO2022227359A1 (en) * | 2021-04-30 | 2022-11-03 | 中国热带农业科学院环境与植物保护研究所 | Method for propagating scutellista caerulea fonscolombe |
| CN113207516A (en) * | 2021-05-31 | 2021-08-06 | 中国热带农业科学院环境与植物保护研究所 | Method for preventing and treating rubber tree lecanium lecanii by using Japanese Lecanicillium lecanii |
| CN113207516B (en) * | 2021-05-31 | 2022-11-25 | 中国热带农业科学院环境与植物保护研究所 | Method for preventing and treating rubber tree lecanium lecanii by using Japanese Lecanicillium lecanii |
| CN118140876A (en) * | 2024-04-22 | 2024-06-07 | 中国热带农业科学院环境与植物保护研究所 | Method for breeding mealybug and flea bees by using mealybug |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106172248B (en) | 2017-09-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102172231B (en) | Method for overwintering species conservation propagation of myzus persicae | |
| CN106172248A (en) | A kind of expanding propagation method of Japan food a red-spotted lizard aphid chalcid fly | |
| CN102125002B (en) | Method for artificially raising plant bug parasitic wasps | |
| CN1954669B (en) | Artificial feeding and generation method of Ectropis oblique hypulin W. and artificial feed for its larva | |
| CN103314786B (en) | Method for cultivating cordyceps indoors | |
| CN103155906A (en) | Aphidoletes aphidimyza mass reproduction technology and application technology | |
| CN102870742A (en) | Large-scale artificial feeding method for eretmocerus hayati | |
| CN113080147B (en) | Method for preventing and treating yellow leaf borers by trichogramma | |
| CN103109781A (en) | Artificial breeding method of Taiwan abdominal cocoon bee | |
| CN102524193A (en) | Large-scale raising method of dominant parasitoid wasps of liriomyza sativae | |
| CN101595857B (en) | Artificial large-scale feeding method of ophraella communa lesage | |
| CN104872068B (en) | Worm device and method for breeding are supported in a kind of Huang chest thrips room | |
| CN105165313B (en) | Aphidius gifuensis short cycle mating system | |
| CN108401903A (en) | A method of improving barley microspores culture callus yield and green seedling | |
| CN105993952B (en) | Pig blood wood cultivates seedling fast breeding method | |
| CN106900538A (en) | A kind of method that the onion production of hybrid seeds is carried out using fly | |
| CN101507425A (en) | Diadegma semiclausum Hellen scale standard breeding technique | |
| CN103858827A (en) | Method for carrying out indoor large-scale diadegma semiclausum hellen breeding | |
| CN102499189B (en) | Method for subculturing Ectropis obliqua hypulina Wehrli indoor with fresh leaves | |
| CN101019522A (en) | Large scale artificial spotted butterfly cultivating technology | |
| CN106613969B (en) | A kind of method of seat grass forming seedling through one step culture mass production | |
| CN107810926A (en) | The artificial large-scale breeding method of Wan Shi Eimeria aythyaes | |
| CN102124999B (en) | Method for raising experimental population of Rhynchophorus ferrugineus | |
| CN107396891A (en) | A kind of method that constant low temperature stores the pale yellow female pupa of grace aphid chalcid fly | |
| CN104886009B (en) | A kind of moth class low instar larvae indoor culture method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |