CN1954669B - Artificial feeding and generation method of Ectropis oblique hypulin W. and artificial feed for its larva - Google Patents
Artificial feeding and generation method of Ectropis oblique hypulin W. and artificial feed for its larva Download PDFInfo
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Abstract
The present invention discloses an artificial culture and sub culture method for the Ectropis oblique hypulin Wehrli, including the following steps: (1) burying the larvas in sandy soil after the pupas of Ectropis oblique hypulin Wehrlis become larvae for 2 days, thus becoming adult through eclosion at room temperature; (2) pairing male adult with female adult, and putting them in a culture container containing nutritive liquid for mating and spawning; (3) carrying out conventional disinfection of ova; (4) inoculating the larvae into a feeding container having feed for culturing the larvae of the Ectropis oblique hypulin Wehrli and culturing the larvae in a healthy insectary, wherein the feed for culturing the larvae of the Ectropis oblique hypulin Wehrli is proportionally prepared from 10.5 to 12.2 parts of wheat's germinal bud, 2 to 4 parts of casein, 0.3 to 1.5 parts of Wei's salt, 0.2 to 0.4 parts of sorbic acid, 0.1 to 0.36 parts of nipagin, 0.48 to 0.88 parts of VC, 0.17 to 0.78 parts of VB, 1.2 to 1.8 parts of agar, 0.05 to 0.08 parts of inositol and 78 to 85 parts of water; and (5) regulating feed 1-5 times and culturing until the larvae be pupated.
Description
Technical field
The present invention relates to a kind of tea geometrid artificial feeding and propagating method.
Background technology
Tea geometrid (Ectropis oblique Warren) is distributed in provinces such as Anhui, Jiangsu, Zhejiang, Jiangxi, Hubei, Hunan, Sichuan, Guangdong and Fujian.Be one of China tea tree primary pest, both endangered spring tea and also endangered the summer autumn tea that the annual harm phase reaches 7-8 month, except that the harm tea tree, still endanger plants such as cherry, pomegranate, Chinese ash.
Tea geometrid stings the food blade with larva causes harm, and blade is stung the food camber incise, and when wildness takes place, often with the whole foods of blade to the greatest extent, only stays bald branch, and owing to this worm generation algebraically is many, breeding is fast, spreads rapidly, often breaks out and causes disaster.Quality and the output and the tree vigo(u)r of tealeaves have seriously been influenced.
Tealeaves originates from China, and the long history more than 3,000 years is arranged, and is classified as the gentle good medicine and the health drink of daily life indispensability already by China common people, is one of world's " three big soft drinks ", is subjected to liking of domestic and international numerous people deeply.Because the tea tree insect is increasing, cause the underproduction of tealeaves, so people are with a large amount of chemical pesticide control insect pests, the long-term use of chemical pesticide, cause insect to develop immunity to drugs, so just have to strengthen the dosage that chemical pesticide uses, ecotope (soil, water source etc.) is caused severe contamination, other biological is constituted a threat to.And the harmful substance of residual heterogeneity in the tealeaves then directly threatens the healthy of people.China is decided to be the high countries and regions of residue of pesticide by European Union, and Japan is to 144 residual limiting of farming, and this will be obstructed China's tea export comprehensively, directly have influence on earn foreign exchange total value and tea grower's economic interests.
Utilize biological insecticides controls tea geometrid, for example: natural enemy, microorganism etc., not only can control that tea geometrid does not cause disaster but also can not produce harmful substance, can guarantee in pest control that tea quality is without prejudice.Biological insecticides retain more stable in natural environment, and it is good to continue control efficiency; Use continuously and be difficult for developing immunity to drugs; To people, animal and wildlife and useful insect is safe and reliable.Therefore in farming, forestry pest control, obtain application more and more widely.
The specificity of biological insecticides is stronger, can only be in the host of living or the cell internal breeding, in other words, can only be by the host insect breeding of living.Produce biological insecticides at present and adopt the field inoculation mode more, its shortcoming is to be subjected to the factor of external environment condition restriction many, as: season, temperature, humidity, the region, wind and rain, sand and dust, insect density, the height of tree, physical features etc., the capital brings bad influence for the production of biological insecticides, cause the output of biological insecticides extremely unstable, and equally also to lose a large amount of leaves with the natural feed breeding grub, mature larva buries successively and pupates to survive the winter after particularly general regional October, March in next year, the early and middle ten days adult eclosion was laid eggs, the phase of surviving the winter reaches 4-5 month, worm source out of stock makes comparatively continuous large-scale production be difficult to more carry out.
All contain a considerable amount of tea leaf powders in the existing tea geometrid larva artificial feed, do not have this composition, tea geometrid just is difficult to finish the whole generation, can't accomplish continuous raising.Therefore use this artificial feed, equally also can lose a large amount of tealeaves.
Summary of the invention
It is high and be suitable for the tea geometrid artificial feeding and the propagating method of factory farming to the purpose of this invention is to provide a kind of pupa and incubation rate.
For achieving the above object, the present invention takes following design:
A kind of tea geometrid artificial feeding and propagating method, its method step is as follows:
(1) in the tea geometrid larvae pupation in the time of 2 days was packed it into sandy soil, adult sprouted wings under the room temperature;
(2) the adult male and female after the above-mentioned emergence are mixed into right, put into the container that has nutrient solution and carry out mate and oviposit;
(3) with the ovum routine disinfection behind the above-mentioned mate and oviposit;
(4) the larva access of hatching is equipped with in the tea geometrid larva raising container of tea geometrid larva artificial feed, puts into healthy supporting between worm and raise; Contained component of described tea geometrid artificial feed and percentage by weight thereof are as follows: wheat germ 10.5-12.2; Casein 2-4; Webster salt 0.3-1.5; Sorbic acid 0.2-0.4; Nipalgin 0.1-0.36; Vitamin C 0.48-0.88; Cobastab 0.17-0.78; Agar 1.2-1.8; Inositol 0.05-0.08; Water 78-85;
(5) brew 1～5 time in whole larva raising process is raised until larvae pupation.
Wherein the sandy soil in the step (1) are moistening sandy soil, and room temperature 25 degree are advisable.
It is good only putting 1 pair of adult in the container that fills nutrient solution in the described step (2).
It is for 2 times good allocating (replacing) feed in the described step (5) in whole larva raising process, to keep the sufficient and fresh of forage volume.
Advantage of the present invention is:
1, can realize the artificial feeding of indoor tea geometrid and go down to posterity, overcome the various undesirable elements that biological insecticides are produced due to the open-air breed tea geometrid, can effectively guarantee output and quality that biological insecticides are produced, the insecticide of preparing is safe and efficient.
2, utilize this raising and the technology that goes down to posterity, larva survival rate height, the phase of going through are short, pupa rate height, and finishing a generation shortened greatly than the open-air corresponding cycle.
3, tea geometrid larva artificial feed provided by the invention, its do not contain host plant any branch, leaf, do, flower, fruit and extract, the raw material wide material sources are not subjected to the restriction in region and season, can produce in a large number, guarantee supply.
Embodiment
Tea geometrid artificial feed of the present invention are raised and the method ratio of the existing artificial single feed for nursing of propagating method, have all done elaborately plannedly at living environment, feed and the selection of the tea geometrid liquid that has additional nutrients, and by result of the test, have also verified its effect of giving prominence to.
At first from following table 1 and table 2, can find out the selection reason of feed formula of the present invention:
The percentage by weight of each material of table 1 tea geometrid larva artificial feed different formulations
| Element (%) | Prescription 1 | Prescription 2 | Prescription 3 | Prescription 4 | Prescription 5 |
| Wheat germ | 10.5 | 11.3 | 12.2 | 9.2 | 13.8 |
| Casein | 2 | 2.7 | 4 | 1 | 8 |
| Webster salt | 0.3 | 0.9 | 1.5 | 0.1 | 2.6 |
| Sorbic acid | 0.2 | 0.28 | 0.40 | 0.1 | 0.6 |
| Nipalgin | 0.1 | 0.2 | 0.36 | 0.1 | 0.5 |
| Vitamin C | 0.48 | 0.67 | 0.88 | 0.2 | 1.0 |
| Cobastab | 0.17 | 0.38 | 0.78 | 0.1 | 0.9 |
| Agar | 1.2 | 1.5 | 1.8 | 1.1 | 1.8 |
| Inositol | 0.05 | 0.07 | 0.08 | 0.1 | 0.8 |
| Water | 85 | 82 | 78 | 88 | 70 |
Table 2: the DIFFERENT FEED prescription is raised the effect of tea geometrid larva
(wheat germ 10.5-12.2 when the present invention is used for the prescription of larva artificial feed of tea geometrid artificial feeding and propagating method and percentage by weight thereof and is selected in this scope; Casein 2-4; Webster salt 0.3-1.5; Sorbic acid 0.2-0.4; Nipalgin 0.1-0.36; Vitamin C 0.48-0.88; Cobastab 0.17-0.78; Agar 1.2-1.8; Inositol 0.05-0.08; Water 78-85), can guarantee effectively that the larva survival rate is more than 70% under this raising method and environment; Larva is gone through the phase and is lower than 12 days; The pupa rate is higher than 72%; Finish one from generation to generation than open-airly shortening 19 days (common open-air tea geometrid finish a generation approximately need about 30 days), the relative cost reduction; Because these raw material are not subjected to the restriction in region and season; at any time suit measures to local conditions to prepare; seasonal shortage can not occur and have influence on the artificial feeding of tea geometrid larva; can be thereby ensured indoor continuous mass rearing tea geometrid larva; both can generation how handed down through generations, can form continuous scale biological insecticides again and produce chain.
Secondly, on the living environment of tea geometrid, through test of many times, in artificial feeding the tea geometrid pupa packed in the moistening sandy soil, remove in 25 degree diapause, the adult that can sprout wings under this environmental condition and the temperature, can significantly be improved incubation rate.For healthy supporting between worm set up in the nursing of larva, this health supports that cleanliness factor is thousand grades between worm, has functions such as temperature control, control light application time and air circulation, answers temperature control 22～25 ℃, control light application time 12～14 hours in tea geometrid artificial feeding of the present invention.
Another important innovations point of the inventive method is: it is right in step (2) the adult male and female of sprouting wings to be mixed into, and puts into container and feeds with special nutrient solution, and only put 1 pair of adult in every container.So promptly guaranteed the supply of nutrition, avoided many again a mutual interference, the ovum that produces of mating thus, egg laying amount than Ensure Liquid liquid not on average exceed 34/female; Incubation rate also exceeds 2 times.Experimental data can be referring to table 3.Wherein the special nutrient solution of the present invention can be selected for use: nutrient solution 1---the 1-10% sucrose solution; Nutrient solution 2---1-5% sucrose solution+1-3%Vc solution; Nutrient solution 3---1-5% sucrose solution+1-3%WS (Wei Shi) salting liquid; Nutrient solution 4---1-5%WS salting liquid+1-3%Vc solution; Nutrient solution 5---4-8%Vc solution.
Table 3: Different Nutrition liquid is raised adults of ectropis obliqua egg laying amount, incubation rate contrast
| Numbering | Total female borer population (head) | Average egg laying amount (grain) | Incubation rate (%) |
| No. 1 liquid | 20 | 106 | 24 |
| No. 2 liquid | 20 | 94 | 45 |
| No. 3 liquid | 20 | 98 | 60 |
| No. 4 liquid | 20 | 34 | 20 |
| No. 5 liquid | 20 | 87 | 72 |
| Contrast | 20 | 53 | 24 |
" contrast " hurdle is not for mending egg laying amount, the incubation rate of raising adults of ectropis obliqua into nutrient solution in the table 3
In tea geometrid artificial feeding of the present invention and propagating method, before dropping into, each larva artificial feed heats mixing earlier, divide again to install in the tea geometrid larva raising container.Help feed of tea geometrid larva and assimilating of various nutrition like this.
The present invention will be further described below in conjunction with specific embodiment:
Embodiment 1:
8000 on selected tea geometrid ovum of initial stage.The whole routine disinfections of tea geometrid ovum grain (were soaked 1 hour with 10% formalin solution earlier, clean with flushing with clean water then, dry in the shade and get final product), under 25 ℃ of conditions, 6080 of hatching larvas, the tea geometrid larva artificial feed is heated mixings according to the prescription in the table 12, divide and install in the tea geometrid larva raising container, newly hatched larvae is inserted (inserting 30-50 newly hatched larvae in every container), putting into healthy supporting between worm raises, look the every interval of feed situation certain hour and change the interpolation feed, pupa was 4356 in 11 days, pupa was put into moistening sandy soil (humidity is held agglomeratingly to loose one's grip can scatter substantially with hand and is advisable) 8 days, 3910 of emergence adults, 1253 of wherein female worms, 2657 of male worms, per 1 pair of adult is put into 1 container, and (putting into a diameter in every container is the aseptic cotton balls of 1.5cm, and nutrition is added in the liquid cotton balls to add No. 5 liquid of nutrition, be advisable not flow out, changed once in 1-2 days), egg laying amount is 110264,5 days ovum phases, 80490 of hatching larvas, reserve seed for planting by raising method of the present invention and when continue using prescription 2 described tea geometrid larva artificial feeds to support larva to 3 ages again 12000 of worms remain 68490 larva replication-competent virus insecticides, can be applied to 4500 mu.
Embodiment 2:
12280 on tea geometrid ovum, raising method and environmental condition are with embodiment 1, under 25 ℃ of conditions, 8596 of hatching larvas, the tea geometrid larva artificial feed divides to install in the tea geometrid larva raising container according to prescription 1 heating mixing, and newly hatched larvae is inserted, putting into healthy supporting between worm raises, pupa was 6018 in 12 days, and pupa was put into moistening sandy soil 9 days, 5115 of emergence adults, 1790 of wherein female worms, 3325 of male worms, per 1 pair of adult is put into 1 container, adds No. 5 liquid of nutrition, egg laying amount is 152150,5 days ovum phases, 111069 of larvas of hatching, 10000 of the worms of reserving seed for planting when supporting to 3 ages, remain 101069 larva replication-competent virus insecticides, can be applied to 6700 mu.
Embodiment 3:
23140 on tea geometrid ovum, raising method and environmental condition are with embodiment 1, under 25 ℃ of conditions, 16429 of hatching larvas, the tea geometrid larva artificial feed divides to install in the tea geometrid larva raising container according to prescription 3 heating mixings, and newly hatched larvae is inserted, putting into healthy supporting between worm raises, pupa was 11664 in 11 days, and pupa was put into moistening sandy soil 8 days, 9915 of emergence adults, 3966 of wherein female worms, 5949 of male worms, per 1 pair of adult is put into 1 container, adds No. 5 liquid of nutrition, egg laying amount is 341076,5 days ovum phases, 255807 of larvas of hatching, 10000 of the worms of reserving seed for planting when supporting to 3 ages, remain 245807 larva replication-competent virus insecticides, can be applied to 15000 mu.
Embodiment 4:
6500 on tea geometrid ovum, raising method and environmental condition are with embodiment 1, under 25 ℃ of conditions, 4680 of hatching larvas, the tea geometrid larva artificial feed divides to install in the tea geometrid larva raising container according to prescription 3 heating mixings, and newly hatched larvae is inserted, putting into healthy supporting between worm raises, pupa was 3416 in 11 days, and pupa was put into moistening sandy soil 8 days, 3041 of emergence adults, 1398 of wherein female worms, 1643 of male worms, per 1 pair of adult is put into 1 container, adds No. 3 liquid of nutrition, egg laying amount is 135606,5 days ovum phases, 81364 of larvas of hatching, 10000 of the worms of reserving seed for planting when supporting to 3 ages, remain 71364 larva replication-competent virus insecticides, can be applied to 4500 mu.
Embodiment 5:
13000 on tea geometrid ovum, raising method and environmental condition are with embodiment 1, under 25 ℃ of conditions, 9620 of hatching larvas, the tea geometrid larva artificial feed divides to install in the tea geometrid larva raising container according to prescription 2 heating mixings, and newly hatched larvae is inserted, putting into healthy supporting between worm raises, pupa was 6830 in 11 days, and pupa was put into moistening sandy soil 8 days, 6215 of emergence adults, 2734 of wherein female worms, 3481 of male worms, per 1 pair of adult is put into 1 container, adds No. 3 liquid of nutrition, egg laying amount is 267932,5 days ovum phases, 163438 of larvas of hatching, 10000 of the worms of reserving seed for planting when supporting to 3 ages, remain 153438 larva replication-competent virus insecticides, can be applied to 10200 mu.
Embodiment 6:
9500 on tea geometrid ovum, raising method and environmental condition are with embodiment 1, under 25 ℃ of conditions, 7125 of hatching larvas, the tea geometrid larva artificial feed divides to install in the tea geometrid larva raising container according to prescription 1 heating mixing, and newly hatched larvae is inserted, putting into healthy supporting between worm raises, pupa was 5058 in 11 days, and pupa was put into moistening sandy soil 8 days, 4653 of emergence adults, 2186 of wherein female worms, 2467 of male worms, per 1 pair of adult is put into 1 container, adds No. 2 liquid of nutrition, egg laying amount is 207670,5 days ovum phases, 91374 of larvas of hatching, 10000 of the worms of reserving seed for planting when supporting to 3 ages, remain 81374 larva replication-competent virus insecticides, can be applied to 5000 mu.
Can be found out by above each embodiment: adopt tea geometrid artificial feeding of the present invention and propagating method and larva artificial feed, nutrient solution, larva survival rate height, the phase of going through are lacked pupa rate height; Because the batching in the feed is the health material prescription, not only makes the tea geometrid larval growth fast, can also guarantee that the insecticide of preparing is safe and efficient.
Claims (7)
1. tea geometrid artificial feeding and propagating method is characterized in that method step is as follows:
(1) in the tea geometrid larvae pupation in the time of 2 days is packed it into sandy soil with the adult that sprouts wings under the room temperature;
(2) the adult male and female after the above-mentioned emergence are mixed into right, put into the container that has nutrient solution and carry out mate and oviposit;
(3) with the ovum routine disinfection behind the above-mentioned mate and oviposit;
(4) the larva access of hatching is equipped with in the tea geometrid larva raising container of tea geometrid larva artificial feed, puts into healthy supporting between worm and raise; Contained component of described tea geometrid artificial feed and percentage by weight thereof are as follows: wheat germ 10.5-12.2; Casein 2-4; Webster salt 0.3-1.5; Sorbic acid 0.2-0.4; Nipalgin 0.1-0.36; Vitamin C 0.48-0.88; Cobastab 0.17-0.78; Agar 1.2-1.8; Inositol 0.05-0.08; Water 78-85;
(5) brew 1～5 time in whole larva raising process is raised until larvae pupation.
2. tea geometrid artificial feeding according to claim 1 and propagating method is characterized in that: wherein the sandy soil in the step (1) are moistening sandy soil, and room temperature is 25 degree.
3. tea geometrid artificial feeding according to claim 1 and propagating method is characterized in that: put 1 pair of adult in the container that fills nutrient solution in the described step (2).
4. tea geometrid artificial feeding according to claim 1 and propagating method is characterized in that: change feed 2 times in the described step (5) in whole larva raising process.
5. tea geometrid artificial feeding according to claim 1 and propagating method is characterized in that: described nutrient solution is 1-10% sucrose solution or 1-5% sucrose solution+1-3%Vc solution, 1-5% sucrose solution+1-3%WS salting liquid, 1-5%WS salting liquid+1-3%Vc solution, 4-8%Vc solution.
6. tea geometrid artificial feeding according to claim 1 and propagating method is characterized in that: cleanliness factor is thousand grades between the foster worm of described health, has temperature control, control light application time and air circulation function.
7. tea geometrid artificial feeding according to claim 1 and propagating method is characterized in that: heat mixing before each larva artificial feed drops into earlier, divide to install in the tea geometrid larva raising container again.
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Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101703172B (en) * | 2009-11-09 | 2012-05-23 | 中国热带农业科学院椰子研究所 | Semi-artificial feed for larva of rhynchophorus ferrugineus |
| CN102550490B (en) * | 2012-01-06 | 2013-09-25 | 湖北省农业科学院果树茶叶研究所 | Method of raising ectropis oblique warren through sambucus chinensis and Ectropis grisescens nucleopolyhedrovirus (EcobNPV) propagation method utilizing same |
| CN103563856B (en) * | 2013-10-28 | 2015-09-30 | 中国农业科学院茶叶研究所 | The collection of ovum and store method in a kind of tea geometrid mass rearing |
| CN103719611A (en) * | 2013-12-24 | 2014-04-16 | 武汉楚强生物科技有限公司 | Feed for breeding ectropis obliqua, preparation method of feed, and breeding method of ectropis obliqua |
| CN103947879B (en) * | 2014-04-28 | 2015-10-28 | 信阳农林学院 | A kind of tea geometrid feed and preparation method thereof |
| CN104430539A (en) * | 2014-11-18 | 2015-03-25 | 江西省科学院微生物研究所 | Ectropis oblique warren nuclear polyhedrosis virus insecticidal suspending agent and production method thereof |
| CN106614395A (en) * | 2016-11-22 | 2017-05-10 | 中国农业科学院茶叶研究所 | Method suitable for oviposition of ectropis grisescens and checking survival rate of eggs |
| CN106962695B (en) * | 2017-03-29 | 2020-08-04 | 中国农业科学院茶叶研究所 | Tea geometrid artificial feed, preparation method and tea geometrid feeding method |
| CN108935347B (en) * | 2018-10-12 | 2021-04-09 | 信阳农林学院 | Breeding method of griseofulvin |
| CN109526890A (en) * | 2019-01-14 | 2019-03-29 | 福建省农业科学院植物保护研究所 | A kind of method of interior mass rearing tea geometrid |
| CN112674242A (en) * | 2019-10-25 | 2021-04-20 | 中国林业科学研究院森林生态环境与保护研究所 | Artificial feed for larval of larval of larval |
| CN115104469A (en) * | 2022-07-18 | 2022-09-27 | 信阳农林学院 | Application of temperature in gray tea geometrid prevention and control and method for gray tea geometrid |
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