CN108935347B - Breeding method of griseofulvin - Google Patents
Breeding method of griseofulvin Download PDFInfo
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- CN108935347B CN108935347B CN201811191289.4A CN201811191289A CN108935347B CN 108935347 B CN108935347 B CN 108935347B CN 201811191289 A CN201811191289 A CN 201811191289A CN 108935347 B CN108935347 B CN 108935347B
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- 238000009395 breeding Methods 0.000 title claims abstract description 34
- IIUZTXTZRGLYTI-UHFFFAOYSA-N Dihydrogriseofulvin Natural products COC1CC(=O)CC(C)C11C(=O)C(C(OC)=CC(OC)=C2Cl)=C2O1 IIUZTXTZRGLYTI-UHFFFAOYSA-N 0.000 title claims abstract description 8
- UXWOXTQWVMFRSE-UHFFFAOYSA-N Griseoviridin Natural products O=C1OC(C)CC=C(C(NCC=CC=CC(O)CC(O)C2)=O)SCC1NC(=O)C1=COC2=N1 UXWOXTQWVMFRSE-UHFFFAOYSA-N 0.000 title claims abstract description 8
- DDUHZTYCFQRHIY-UHFFFAOYSA-N Negwer: 6874 Natural products COC1=CC(=O)CC(C)C11C(=O)C(C(OC)=CC(OC)=C2Cl)=C2O1 DDUHZTYCFQRHIY-UHFFFAOYSA-N 0.000 title claims abstract description 8
- DDUHZTYCFQRHIY-RBHXEPJQSA-N griseofulvin Chemical compound COC1=CC(=O)C[C@@H](C)[C@@]11C(=O)C(C(OC)=CC(OC)=C2Cl)=C2O1 DDUHZTYCFQRHIY-RBHXEPJQSA-N 0.000 title claims abstract description 8
- 229960002867 griseofulvin Drugs 0.000 title claims abstract description 8
- 241000205585 Aquilegia canadensis Species 0.000 claims abstract description 49
- 241001046947 Ectropis obliqua Species 0.000 claims abstract description 46
- 244000057114 Sapium sebiferum Species 0.000 claims abstract description 35
- 235000005128 Sapium sebiferum Nutrition 0.000 claims abstract description 35
- 241000238631 Hexapoda Species 0.000 claims abstract description 31
- 239000000843 powder Substances 0.000 claims abstract description 25
- 230000001488 breeding effect Effects 0.000 claims abstract description 22
- 238000000034 method Methods 0.000 claims abstract description 18
- 239000007864 aqueous solution Substances 0.000 claims abstract description 17
- 230000032669 eclosion Effects 0.000 claims abstract description 10
- 235000013601 eggs Nutrition 0.000 claims abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 37
- 241001122767 Theaceae Species 0.000 claims description 18
- 235000009024 Ceanothus sanguineus Nutrition 0.000 claims description 5
- 240000003553 Leptospermum scoparium Species 0.000 claims description 5
- 235000015459 Lycium barbarum Nutrition 0.000 claims description 5
- 230000003203 everyday effect Effects 0.000 claims description 5
- 230000003716 rejuvenation Effects 0.000 claims description 5
- 229920000742 Cotton Polymers 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 claims description 4
- 235000012907 honey Nutrition 0.000 claims description 3
- 235000016709 nutrition Nutrition 0.000 claims description 3
- 230000035764 nutrition Effects 0.000 claims description 3
- 239000000575 pesticide Substances 0.000 claims description 3
- 230000001502 supplementing effect Effects 0.000 claims description 3
- 238000011161 development Methods 0.000 abstract description 5
- 230000018109 developmental process Effects 0.000 abstract description 5
- 239000000463 material Substances 0.000 abstract description 4
- 244000269722 Thea sinensis Species 0.000 abstract 1
- 230000000052 comparative effect Effects 0.000 description 9
- 241001116129 Ectropis grisescens Species 0.000 description 5
- 238000012986 modification Methods 0.000 description 4
- 230000004048 modification Effects 0.000 description 4
- 230000004083 survival effect Effects 0.000 description 4
- 238000012136 culture method Methods 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 241000607479 Yersinia pestis Species 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000013011 mating Effects 0.000 description 2
- 206010063746 Accidental death Diseases 0.000 description 1
- 206010013647 Drowning Diseases 0.000 description 1
- 241001046946 Ectropis Species 0.000 description 1
- 241001634830 Geometridae Species 0.000 description 1
- 241000255777 Lepidoptera Species 0.000 description 1
- 241000382353 Pupa Species 0.000 description 1
- 241000256247 Spodoptera exigua Species 0.000 description 1
- 241000006350 Turraea heterophylla Species 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000002347 injection Methods 0.000 description 1
- 239000007924 injection Substances 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 230000017448 oviposition Effects 0.000 description 1
- AJTPODXVRWXEMS-UHFFFAOYSA-M potassium;4-(1h-indol-2-yl)butanoate Chemical group [K+].C1=CC=C2NC(CCCC(=O)[O-])=CC2=C1 AJTPODXVRWXEMS-UHFFFAOYSA-M 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000019617 pupation Effects 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000009897 systematic effect Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Feed For Specific Animals (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a method for breeding gray tea geometrid, which comprises the following steps: inserting honeysuckle branches into the rooting powder aqueous solution; inoculating the ash tea geometrid larvae to honeysuckle branches for feeding; after the larvae are 2-3 instars, replacing the honeysuckle branches with the Chinese tallow tree branches, and transferring the larvae originally bred on the honeysuckle branches to the Chinese tallow tree branches for breeding; after the larva grows on the Chinese tallow tree branches to pupate, picking the pupae out; after pupae eclosion into imagoes, the imagoes mate in the insect breeding cage; allowing mated adults to lay eggs on the honeysuckle branches; and repeating the steps circularly to realize the subculture of the indoor unnatural host tea leaves of the griseofulvin. The breeding method adopts honeysuckle branches to breed 1-2 instar larvae, and Chinese tallow tree branches to breed 2-3 instar larvae, compared with breeding by a single host or artificial feed, the breeding method has the advantages of low cost, easy material acquisition, good larva growth and development, low mortality rate and life habit close to field loopers.
Description
Technical Field
The invention belongs to the technical field of artificial breeding of ectropis obliqua, and particularly relates to a breeding method of the ectropis obliqua.
Background
The ash tea geometrid (Ectropis grissescens Warren) belongs to Lepidoptera (Lepidotera) family (Geometridae) and is distributed in Zhejiang, Hubei, Hunan, Fujian, Jiangxi, Jiangsu, Anhui, Henan, Guangxi, Guangdong and other provinces, and almost covers all tea-producing provinces in China. The ash tea geometrid eats young leaves, mature leaves and old leaves of tea trees by larvae, and when the larvae are more likely to eat, the young shoots are eaten after the leaves are polished, so that the tea trees become polished rods and are burnt, and the tree vigor and the yield are seriously influenced. Since the morphological characteristics of the gray tea geometrid and the tea geometrid (e.obliqua rout) are very similar, they are difficult to distinguish in production and sometimes confuse. The influence of the ash tea geometrid on a tea garden is determined to be larger, but the research of the ash tea geometrid is always more important, and the ash tea geometrid is rarely concerned. Therefore, the systematic research on the gray tea geometrid has very important economic and scientific values.
In view of the serious harm of the ectropis grisescens, in recent years, many insect scholars are exploring indoor feeding methods of the ectropis grisescens, mainly comprising artificial feed formulas, water culture method for breeding tea leaves, fresh leaf subculture feeding methods and the like, most of the artificial feed formulas in actual operation have the phenomena of high cost, low larva survival rate, high pupation distortion rate and the like, and cannot be widely applied to large-scale feeding of the ectropis grisescens. The common water culture method increases the probability of drowning death of larvae, and inevitably increases the phenomenon of loss or death of the gray tea geometrid in the transfer process, and in the subculture of the sand-soil inserted fresh paper slips, an indoor air conditioner is always opened to maintain the temperature of a pest breeding room, so that the sand-inserted slips are easy to wither, and meanwhile, the labor, time and energy are wasted. Moreover, at present, all studies on feeding of fresh leaves of the gray tea geometrid are concentrated on tea leaves of natural hosts, and in actual work, a large amount of fresh tea leaves are difficult to collect uninterruptedly, and the generation, prevention and control studies of the gray tea geometrid need to solve the problem of artificial mass feeding of the gray tea geometrid, so that a fresh-leaf host and a feeding method for replacing the fresh tea leaves need to be found urgently in production.
Disclosure of Invention
The invention aims to provide an indoor non-tea-leaf host subculture method for ectropis obliqua, which is easy to obtain materials, high in larva survival rate and good in larva growth and development, aiming at the defects of the prior art.
In order to achieve the aim, the invention provides a method for raising griseus inchworm, which comprises the following steps:
step 1, adding rooting powder into clear water for dissolving to obtain a rooting powder aqueous solution, adding the rooting powder aqueous solution into a conical flask, inserting honeysuckle branches into the conical flask, and changing the rooting powder aqueous solution in the conical flask into clear water after 2 hours;
wherein the mass ratio of the rooting powder to the clear water in the rooting powder aqueous solution is 100 mg: 1 kg;
step 2, inoculating insects: transferring the conical flask in the step 1 into an insect cage, and inoculating first-generation first-instar larvae obtained after rejuvenation of field collected ash tea geometrid larvae and indoor fresh tea geometrid cultured ash tea geometrid into honeysuckle branches in the insect cage for feeding;
step 3, insect breeding: filling water into the conical flask to full according to the consumption condition of clear water in the conical flask every day, and replacing the honeysuckle branches in the conical flask according to the eating condition of the leaves on the honeysuckle branches;
step 4, after the larvae are aged for 2-3 years, replacing the honeysuckle branches in the conical flask with the Chinese tallow tree branches, then transferring the larvae originally bred on the honeysuckle branches to the Chinese tallow tree branches, filling water into the conical flask to the full degree according to the consumption condition of clear water in the conical flask, and replacing the Chinese tallow tree branches according to the condition that the leaves on the Chinese tallow tree branches are eaten;
step 5, pupating: after the larva grows on the Chinese tallow tree branches to pupate, picking the pupae out, sterilizing the pupae under an ultraviolet lamp for 10min, and putting the pupae into an insect cage for eclosion;
step 6, feathering: after pupa eclosion becomes an adult, mating the adult in an insect breeding cage, replacing a Chinese tallow tree branch in the conical flask with a honeysuckle branch, and allowing the mated adult to lay eggs on the honeysuckle branch;
and 7, repeating the steps 3 to 6, and sequentially circulating to realize the subculture of the indoor unnatural host tea leaves of the griseofulvin.
Preferably, the aqueous solution of the rooting powder in the step 1 is added to the neck of the conical flask.
Preferably, after the honeysuckle branches or the Chinese tallow tree branches are inserted into the conical bottle, gaps between the honeysuckle branches or the Chinese tallow tree branches and the bottle mouth are tightly plugged by absorbent cotton.
Preferably, the size of the cage is 55cm x 55 cm.
Preferably, the aperture of the insect cage is 120 meshes.
Preferably, the ash tea geometrid larvae collected in the field in the step 2 are the ash tea geometrid larvae collected on tea trees which are not applied with pesticide for at least half a year.
Preferably, in step 3 and step 4, the needle tube is used to inject water into the conical flask.
Preferably, in the step 6, 2-3% of honey water is filled in the sponge allocated in the insect rearing cage for supplementing nutrition for adults.
Compared with the prior art, the invention has the beneficial effects that:
1) the 1-2 instar larvae are weak and small, like eating tender leaves, and the honeysuckle flowers with thin, tender and soft leaves are selected as hosts, so that the survival rate of the low-age larvae of the looper obliqua is greatly improved compared with that of the single sapium sebiferum leaf feeding.
2) The feeding conical bottle used by the feeding method is small in mouth, and even if the larva falls into the feeding container after the absorbent cotton is plugged tightly, the larva does not die after entering water; after the rooting powder is added, the honeysuckle or Chinese tallow tree branches are kept fresh continuously, and the roots are generated commonly and can be recycled, so that the disease infection probability of larvae is reduced; compared with the common water culture method, the mortality rate is obviously reduced.
3) The breeding method of the invention can ensure that the whole generation of the ectropis obliqua is completed in one breeding container without transferring the breeding container, thereby reducing the occurrence of loss or accidental death.
4) The breeding method of the invention has large breeding amount and strong operability, for example, 15-20 honeysuckle branches or 4-5 Chinese tallow tree trunk branches can be inserted into a conical flask with the diameter of 500ml, about 500 larvae can be bred, and about 4000 larvae can be bred by placing 5-6 conical flasks in a pest cage.
5) The core of the breeding method is that honeysuckle branches are adopted to breed 1-2 instar larvae, Chinese tallow tree branches are adopted to breed 2-3 instar larvae later, and compared with the breeding method with a single host or artificial feed, the breeding method has the advantages of low cost, easy material obtaining, good larva growth and development, low mortality rate and life habit close to field looper.
Detailed Description
In order to make the technical solutions of the present invention better understood and enable those skilled in the art to practice the present invention, the following embodiments are further described, but the present invention is not limited to the following embodiments.
The experimental methods described in the following examples are all conventional methods unless otherwise specified; the rooting powder is potassium indolebutyrate, is purchased from Anyang Quanfeng biotechnology limited, and other used materials can be purchased in the market without special instructions.
Example 1
A method for breeding ectropis grisescens comprises the following steps:
step 1, adding rooting powder into clear water for dissolving to obtain a rooting powder aqueous solution with the mass concentration of 100mg/1kg, adding the rooting powder aqueous solution into a conical flask to the position of a bottleneck, inserting fresh honeysuckle branches into the conical flask, and changing the rooting powder aqueous solution in the conical flask into the clear water after 2 hours;
step 2, inoculating insects: transferring the conical flask in the step 1 into an insect cage with the size of 55cm x 55cm and the aperture of 120 meshes, and inoculating first-generation first-instar larvae obtained after rejuvenation of the gray tea geometrid cultured by indoor fresh tea leaves onto honeysuckle branches in the insect cage for feeding;
step 3, insect breeding: observing the consumption condition of the clear water in the conical flask every day, and injecting water into the conical flask to full by using a needle tube when the water level of the clear water in the conical flask drops to the bottleneck;
observing the feeding condition of the upper leaves of the honeysuckle branches every day, and replacing the honeysuckle branches in the conical bottle after 90% of the upper leaves of the honeysuckle branches are fed;
step 4, after the larvae are aged for 2-3 years, replacing the honeysuckle branches in the conical flask with the Chinese tallow tree branches, then transferring the larvae originally bred on the honeysuckle branches to the Chinese tallow tree branches, filling water into the conical flask to the full degree according to the consumption condition of clear water in the conical flask, and replacing the Chinese tallow tree branches according to the condition that the leaves on the Chinese tallow tree branches are eaten;
wherein, the consumption condition of the clean water, the judgment of the eating condition of the leaves on the Chinese tallow tree branches and the water injection mode are completely the same as the step 3;
step 5, pupating: after the larva grows on the Chinese tallow tree branches to pupate, picking the pupae out, sterilizing the pupae under an ultraviolet lamp for 10min, and putting the pupae into an insect cage for eclosion;
step 6, feathering: after pupae eclosion becomes adults, the adults mate in an insect cage, 2-3% of honey water is fully absorbed by sponge arranged in the insect cage for supplementing nutrition to the adults, meanwhile, sapium sebiferum branches in a conical flask are replaced by honeysuckle branches, and then the mated adults lay eggs on the honeysuckle branches;
and 7, repeating the steps 3 to 6, and sequentially circulating to realize the subculture of the indoor unnatural host tea leaves of the griseofulvin.
It should be noted that after the honeysuckle branches or the Chinese tallow tree branches are inserted into the conical bottle, gaps between the honeysuckle branches or the Chinese tallow tree branches and the bottle mouth are tightly plugged by absorbent cotton.
Step 2, the field collected ash tea geometrid larvae refer to tea geometrid larvae collected on tea trees without pesticide applied for at least half a year.
In order to illustrate the effect of the invention, the invention is also provided with a comparative example which is specifically as follows:
comparative example 1
A method for breeding ectropis grisescens comprises the following steps:
step 1, adding rooting powder into clear water for dissolving to obtain a rooting powder aqueous solution with the mass concentration of 100mg/1kg, adding the rooting powder aqueous solution into a conical flask to the position of a bottleneck, inserting fresh honeysuckle branches into the conical flask, and changing the rooting powder aqueous solution in the conical flask into the clear water after 2 hours;
step 2, inoculating insects: transferring the conical flask in the step 1 into an insect cage, and inoculating first-generation first-instar larvae obtained after rejuvenation of field collected ash tea geometrid larvae and indoor fresh tea geometrid cultured ash tea geometrid into honeysuckle branches in the insect cage for feeding;
step 3, insect breeding: filling water into the conical flask to full according to the consumption condition of clear water in the conical flask every day, and replacing the honeysuckle branches in the conical flask according to the eating condition of the leaves on the honeysuckle branches;
step 4, pupating: after the larvae grow on the honeysuckle branches to pupate, picking pupae out, sterilizing the pupae under an ultraviolet lamp for 10min, and putting the pupae into an insect cage for eclosion;
step 5, feathering: after pupae eclosion into adults, mating the adults in an insect breeding cage, and then enabling the mated adults to lay eggs on honeysuckle branches;
and 6, repeating the steps 3 to 6, and sequentially circulating to realize the subculture of the indoor unnatural host tea leaves of the griseofulvin.
Comparative example 2
The breeding method of the ectropis obliqua is completely the same as the comparative example 1, except that all the honeysuckle branches used in the comparative example 1 are replaced by the Chinese tallow tree branches.
Comparative example 3
The breeding method of the ectropis obliqua is completely the same as that of the comparative example 1, except that the honeysuckle branches used in the comparative example 1 are completely replaced by the tea branches.
It should be noted that, when the experiment is started, the first generation of first instar larvae is obtained by collecting the gray tea geometrid larvae from the field and breeding the indoor cultivated gray tea geometrid in a rejuvenation and mixing manner, and then indoor unnatural host tea is subcultured, the experiment of the example 1 and the comparative examples 1-3 is completed by starting 6 and 6 days in 2018 and ending 21 and 6 months in 2018, and the first generation breeding of the gray tea geometrid is completed, and the specific breeding results are shown in table 1.
TABLE 1 results of indoor fresh leaf subculture
As can be seen from the table 1, the survival rate of the larvae of the gray tea geometrid bred by the method is almost equal to that of the larvae bred by the fresh tea leaves, the indexes of the larvae in the development period, the weights of male and female pupae, the egg laying amount, the eclosion rate of adults, the egg hatching rate and the like are not greatly changed with the breeding of the fresh tea leaves, the larvae bred by the honeysuckle and the Chinese tallow trees in a matching way are more robust and more active than the larvae bred by the fresh tea leaves, the growth and development are more favorable, and the larvae are not different from the life habits of the gray tea geometrid bred by the fresh tea leaves, so that a large number of orderly standard insect sources can be provided for various researches on the gray tea geometrid, and the practical requirements are met.
While preferred embodiments of the present invention have been described, additional variations and modifications in those embodiments may occur to those skilled in the art once they learn of the basic inventive concepts. Therefore, it is intended that the appended claims be interpreted as including preferred embodiments and all such alterations and modifications as fall within the scope of the invention.
It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, it is intended that such changes and modifications be included within the scope of the appended claims and their equivalents.
Claims (7)
1. The raising method of the griseofulvin is characterized by comprising the following steps of:
step 1, adding rooting powder into clear water for dissolving to obtain a rooting powder aqueous solution, adding the rooting powder aqueous solution into a conical flask, inserting honeysuckle branches into the conical flask, and changing the rooting powder aqueous solution in the conical flask into clear water after 2 hours;
wherein the mass ratio of the rooting powder to the clear water in the rooting powder aqueous solution is 100 mg: 1 kg;
step 2, inoculating insects: transferring the conical flask in the step 1 into an insect cage, and inoculating first-generation first-instar larvae obtained after rejuvenation of field collected ash tea geometrid larvae and indoor fresh tea geometrid cultured ash tea geometrid into honeysuckle branches in the insect cage for feeding;
step 3, insect breeding: filling water into the conical flask to full according to the consumption condition of clear water in the conical flask every day, and replacing the honeysuckle branches in the conical flask according to the eating condition of the leaves on the honeysuckle branches;
step 4, after the larvae are aged for 2-3 years, replacing the honeysuckle branches in the conical flask with the Chinese tallow tree branches, then transferring the larvae originally bred on the honeysuckle branches to the Chinese tallow tree branches, filling water into the conical flask to the full degree according to the consumption condition of clear water in the conical flask, and replacing the Chinese tallow tree branches according to the condition that the leaves on the Chinese tallow tree branches are eaten;
step 5, pupating: after the larva grows on the Chinese tallow tree branches to pupate, picking the pupae out, sterilizing the pupae under an ultraviolet lamp for 10min, and putting the pupae into an insect cage for eclosion;
step 6, feathering: after pupae eclosion becomes adults, the adults mate in an insect cage, 2-3% of honey water is fully absorbed by sponge arranged in the insect cage for supplementing nutrition to the adults, meanwhile, sapium sebiferum branches in a conical flask are replaced by honeysuckle branches, and then the mated adults lay eggs on the honeysuckle branches;
and 7, repeating the steps 3 to 6, and sequentially circulating to realize the subculture of the indoor unnatural host tea leaves of the griseofulvin.
2. The raising method of gray tea geometrid according to claim 1, wherein the aqueous solution of rooting powder is added to the neck of the erlenmeyer flask in step 1.
3. The method for raising loopers, according to claim 1, wherein after the honeysuckle shoots or the sapium sebiferum shoots are inserted into the erlenmeyer flask, gaps between the honeysuckle shoots or the sapium sebiferum shoots and the mouth of the erlenmeyer flask are tightly closed with absorbent cotton.
4. The method for raising looper obliqua according to claim 1, wherein the size of the cage is 55cm by 55 cm.
5. The method for raising loopers of the gray tea according to claim 4, wherein the diameter of the cage is 120 mesh.
6. The method for raising the gray tea geometrid according to claim 1, wherein the gray tea geometrid larvae collected in the field in step 2 are gray tea geometrid larvae collected on tea trees on which a pesticide has not been applied for at least half a year.
7. The method for raising gray tea geometrid according to claim 1, wherein the water is injected into the erlenmeyer flask by using a needle tube in both of the steps 3 and 4.
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