CN102499188B - Agasicles hygrophila experimental population raising method - Google Patents
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- CN102499188B CN102499188B CN 201110364199 CN201110364199A CN102499188B CN 102499188 B CN102499188 B CN 102499188B CN 201110364199 CN201110364199 CN 201110364199 CN 201110364199 A CN201110364199 A CN 201110364199A CN 102499188 B CN102499188 B CN 102499188B
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Abstract
The invention discloses an agasicles hygrophila experimental population raising method. The agasicles hygrophila experimental population raising method includes steps that filter paper with the size equal to that of a bottle bottom is placed in an insect breeding bottle I, and is wetted, and 20-30 adult insects which are just subjected to the eclosion are placed in the insect breeding bottle I and are fed by three fresh alternanthera philoxeroides; alternanthera philoxeroides leaves with egg masses in the insect breeding bottle I are placed in insect breeding bottles II, each insert breeding bottle II holds ten alternanthera philoxeroides leaves, and then the insect breeding bottles II are placed in an artificial incubator; fresh alternanthera philoxeroides is placed in the incubator on the first day of incubation, and the alternanthera philoxeroides is continuously replaced by other fresh alternanthera philoxeroides until larvas are matured; and when about to pupate, the agasicles hygrophila matured larvas are transferred into pupating devices, each pupating device contains 100 matured larvas, the matured larvas enter straws of alternanthera philoxeroides to pupate, and pupating rate can reach 80% to 95%. The agasicles hygrophila experimental population raising method is simple in artificial raising, easy to control, small in occupied space and low in cost, and can be used for raising a controllable quantity of neat and consistent agasicles hygrophila with different insect development stages and different insect ages according to different experimental needs.
Description
Technical field
The present invention relates to the raising method of a kind of insect, particularly relate to the raising method of the straight chest flea beetle experimental population of a kind of lotus grass.
Background technology
Alternanthera philoxeroides (Alternanthera philoxeroides (Mart.) Griseb.) has another name called alternanthera philoxeroides, water peanut, revolution grass etc., belong to Amaranthaceae Amarthantaceae alternanthera and belong to Alternanthera Forsk, originate from South America, being amphibious herbaceos perennial, is a kind of global malignant weed.After invasion China, for want of nature and biological restraining factors are caused disaster in the regional outburst rapidly of major parts such as south.The diversity of the production of serious threat fishery, agricultural and view, Wetland ecological also has influence on human health, water transportation etc. simultaneously.
Find in the biological control research to Alternanthera philoxeroides, in numerous natural enemy insects with the straight chest flea beetle Agasicles hygophila of lotus grass, (coleoptera Coleoptera, Chrysomelidae Chrysomelidae, flea beetle subfamily Halticinae) the most effective, single-minded to the control of Alternanthera philoxeroides, can not threaten to other plant.The Chinese Academy of Agricultural Sciences introduces this natural enemy in 1986 from Fla., it has been done safety evaluation and test after, the fixed point demonstration is also promoted.
The straight chest flea beetle of lotus grass is a kind of with larva and imago feeding Alternanthera philoxeroides blade and tender stem, and mature larva bores the special feeding habits natural enemy that the moth cane is pupated and stoped plant strain growth.The straight chest flea beetle adult of lotus grass is with the leaf back of ovum product at Alternanthera philoxeroides, newly hatched larvae accumulates in the young tender leaf back of the body of Alternanthera philoxeroides more and gets food, forms netted cavity, and 2,3 instar larvaes and adult are then got the food whole blade, under the under-supply situation of blade, also get food stem stalk.3 age mature larva bore in the pulp cavity that moth advances Alternanthera philoxeroides and pupate.After the emergence, the food stem stalk that becomes to damage by worms forms the emergence hole, and therefrom climbs out of.Have the emergence hole stem stalk frangibility, rot, thereby destroy plant.The straight chest flea beetle of lotus grass belongs to high reproductive ability insect, on average whenever femalely lays eggs 1300, and the generation cycle is short, and larva and adult all do not have the autotomy phenomenon, and the adult mean lifetime is January, can set up kind of a population at short notice.The adult diffusivity is strong, has guaranteed the expansion of population, so in can suppressing on a large scale the growth of Alternanthera philoxeroides to reach the purpose of control.
Though the introducing a fine variety of the straight chest flea beetle of lotus grass, field release tech, discharge the selection on ground and discharge management, the method for releasing on ground and manually help to move etc. and reach its maturity.But these populations cause this natural enemy to fail to give full play to its preventive and therapeutic effect in biological control because geography, gene, the first-class basis of biology and application foundation Study on Theory deficiency have hindered research and application process.Yet, China does not form a cover complete skill as yet to the artificial feeding of the straight chest flea beetle experimental population of lotus grass at present, therefore can solve the artificial feeding technology of the straight chest flea beetle experimental population of lotus grass, can accelerate the research and development progress in the fields such as biology, ecology and Prevention Technique of the straight chest flea beetle of lotus grass, to realize the bigger value of its biological control, also for scientific research provides special research material, disclose biological phenomena better.The raising that also can be simultaneously other insect experimental populations provides a kind of scientific methods opinion.
Summary of the invention
The object of the invention is to provide the raising method of the straight chest flea beetle experimental population of a kind of lotus grass.
The present invention is achieved through the following technical solutions:
The raising method of the straight chest flea beetle experimental population of a kind of lotus grass, its step is as follows:
(1) in vial I, put into bottle at the bottom of one of the filter paper of size, running water soaks; Get the adult of firm emergence and put into bottle, every bottle a worm number is the 15-40 head, get the 2-4 root from the fresh Alternanthera philoxeroides cauline leaf of the top 4-8 of containing sheet, and with putting into vial I behind the running water immersion 5-15min, gauze is covered on the vial I mouth, fix with rubber band, be placed in the artificial incubator and cultivate, the temperature of artificial incubator is 20-30 ℃, and illumination and dark duration are than being 10-18h: 8-12h, and humidity is 70%-90%; Early take out vial I before 9 every day in artificial incubator, clamp the stem of residue cauline leaf in the bottle with the tip tweezers, dial gently or shake off adult to vial I with wolf cub hair pen, getting the 2-4 root changes from the fresh Alternanthera philoxeroides cauline leaf of the top 4-8 of containing sheet, and with putting into vial I behind the running water immersion 5-15min, seal then;
(2) begin to lay eggs after the adult 2-4 day of just having sprouted wings, every 18-30 hour with vial I in pieces of an egg and adult separately, the pieces of an egg of Shou Jiing are same batch each time, label to record at the bottle wall and lay eggs the date; Therefrom take out the cauline leaf that has pieces of an egg from vial I, to there be the blade of pieces of an egg to take, put into vial II, be covered with the filter paper that soaks with end size at the bottom of the vial II, put 8-12 sheet pieces of an egg for every bottle, cover bottleneck with preservative film, and sting 10-15 pin hole thereon, add a cover a gauze again, wale bottleneck with rubber band; In time spray running water every day in vial II, moistening whole filter paper keeps the humidity in the vial II; Pieces of an egg were hatched in 4-6 days;
(3) the 1st day after the larva hatching directly adds fresh Alternanthera philoxeroides in vial II; Only remove the old leaf with chorion on the 2nd day, and in vial II, add fresh Alternanthera philoxeroides again; Withered Alternanthera philoxeroides blade pressed from both sides out in the 3rd day, and with little writing brush remaining few larva on the old blade of Alternanthera philoxeroides is swept down lightly, the fresher Alternanthera philoxeroides of adding in the vial II; Add fresh Alternanthera philoxeroides so repeatedly, 2 ages and 3 instar larvaes can directly be swept down larva with wolf cub hair pen or clamp old grass blade with tweezers and shake off larva down in vial II, add fresh Alternanthera philoxeroides again in vial II; The Alternanthera philoxeroides cauline leaf of handling out is dropped into designated treatment point and destroys;
(4) the mature larva action of pupating in advance is slower, food ingestion also reduces gradually, and mature larva is inserted in the device of pupating, and with wolf cub hair pen mature larva is received on the Alternanthera philoxeroides cauline leaf lightly, connect larva as far as possible on cane, insert 80-120 mature larva in each device of pupating; Mature larva pierces in the Alternanthera philoxeroides stem stalk pupates, and it is 20-30 ℃ that the device of will pupating is placed on temperature, and illumination and dark duration are than being 10-18h: 8-12h, and humidity is 70%-90%; Count the adult that emergence was just arranged on the 7th day from the access mature larva and occur, and all sprouted wings in ensuing 4 days, just the cane in the device can be taken out at the 11st day, the mature larva pupation rate can reach 80%-95%.
The raising method of the straight chest flea beetle experimental population of lotus grass of the present invention, its method is preferably as follows step:
(1) in vial I, put into bottle at the bottom of one of the filter paper of size, soak with running water; Get the adult of firm emergence and put into bottle, every bottle a worm number is the 20-30 head, get 3 and contain 6 fresh Alternanthera philoxeroides cauline leafs from top, and with putting into vial I behind the running water immersion 10min, gauze is covered on the vial I mouth, fix with rubber band, be placed in the artificial incubator, the temperature of artificial incubator is 26 ℃, and illumination and dark duration are than being 14h: 10h, and humidity is 85%; Every day early 9: before the some vial I is taken out in artificial incubator, clamp the stem of residue cauline leaf in the bottle with the tip tweezers, dial gently or shake off adult to vial I with wolf cub hair pen, get 3 from the top cauline leaf replacing that contains 6 fresh Alternanthera philoxeroides, and with putting into vial I behind the running water immersion 10min, seal then;
(2) adult that has just sprouted wings began to lay eggs after the 3rd day, every 24 hours with vial I in pieces of an egg and adult separately, the pieces of an egg of Shou Jiing are same batch each time, label to record at the bottle wall and lay eggs the date; From vial I, take out the cauline leaf that has pieces of an egg, to there be the blade of pieces of an egg to take, put into vial II, be covered with the filter paper that soaks with end size at the bottom of the vial II, put 10 pieces of an egg for every bottle, cover with preservative film, and sting 10 pin holes thereon, add a cover a common gauze again, wale bottleneck with rubber band; In time spray running water every day in bottle, moistening whole filter paper keeps the humidity in the bottle; Pieces of an egg were hatching in 3-4 days.
(3) the 1st day after the larva hatching directly adds fresh Alternanthera philoxeroides in vial II; Only remove the old leaf with chorion on the 2nd day, and in vial II, add fresh Alternanthera philoxeroides again; Withered Alternanthera philoxeroides blade pressed from both sides out in the 3rd, and with little writing brush remaining few larva on the old blade of Alternanthera philoxeroides is swept down lightly, the fresher Alternanthera philoxeroides of adding in the vial II; Add fresh Alternanthera philoxeroides so repeatedly, 2 ages and 3 instar larvaes can directly be swept down larva with wolf cub hair pen or clamp old grass blade with tweezers and shake off larva down in vial II, add fresh Alternanthera philoxeroides again in vial II; The Alternanthera philoxeroides cauline leaf of handling out is dropped into designated treatment point and destroys;
(4) the mature larva action of pupating in advance is slower, food ingestion also reduces gradually, and mature larva is inserted in the device of pupating, and with wolf cub hair pen mature larva is received on the Alternanthera philoxeroides cauline leaf lightly, connect larva as far as possible on cane, insert 100 mature larvas in each device of pupating; Mature larva pierces in the Alternanthera philoxeroides stem stalk pupates, and the device laying temperature of will pupating is 26 ℃, and illumination and dark duration be than being 14h: 10h, and humidity is 85% cultivating in artificial incubator; Count the adult that emergence was just arranged on the 7th day from the access mature larva and occur, and adult all sprouts wings in ensuing 4 days, just the cane in the device can be taken out at the 11st day, the mature larva pupation rate can reach 80%-95%.
The device of pupating of the present invention is made up of cloche, hardboard, Alternanthera philoxeroides, vial and the chassis of gauze, " protruding " font; Hardboard is cut into " recessed " word shape, it is covered on vial, with wide adhesive tape it is wrapped on the vial and fixes, in vial, add 9/10 running water; From the greenhouse, get the fresh Alternanthera philoxeroides stem of 8-12 root, the leaf that most end 1-3 is held successively removes, the 0.5-1.5cm place otch of a 1-3cm of cutting sth. askew snarls not make it loose with rubber band outside putting successively apart from last, and the stem that fixes is inserted in the vial; Vial is put into the chassis, and an end of yerbadetajo seed grass blade in the family way outwardly; Crooked Alternanthera philoxeroides cauline leaf makes the top of each stem contact with the chassis, cover upper glass cover; Gauze on the cloche main header casing is fixed with rubber band; Cloche outer wall middle part is labelled, and label substance is for inserting mature larva date and a worm number, if the larva that connect in a day more than or equal to two jars, is then distinguished with A, B, C....
The device of pupating of the present invention is made up of cloche, hardboard, Alternanthera philoxeroides, vial and the chassis of gauze, " protruding " font; Hardboard is cut into " recessed " word shape, it is covered on vial, with wide adhesive tape it is wrapped on the vial and fixes, in vial, add 9/10 running water; Get 10 fresh Alternanthera philoxeroides stems from the greenhouse, the leaf that most end 2 is held successively removes, and the 1cm place otch of a 2cm of cutting sth. askew snarls not make it loose with rubber band outside putting successively apart from last, and the stem that fixes is inserted in the vial; Vial is put into the chassis, and an end of yerbadetajo seed grass blade in the family way outwardly; Crooked Alternanthera philoxeroides cauline leaf makes the top of each stem contact with the chassis, cover upper glass cover; Gauze on the cloche main header casing is fixed with rubber band; Cloche outer wall middle part is labelled, and label substance is for inserting mature larva date and a worm number, if the larva that connect in a day more than or equal to two jars, is then distinguished with A, B, C....
(4) step of the present invention is in the raising stage of pupa, pupates the Alternanthera philoxeroides stem stalk of usefulness for from the top number 6-10 joint altogether that begins for mature larva, the thick 4-6mm of the stem of final section, and the total length of stem is at 40-55cm, and it is longer to choose single-unit, not wounded in the battle stem.
(4) step of the present invention is in the raising stage of pupa, pupates the Alternanthera philoxeroides stem stalk of usefulness for from top number totally 8 joints that begin for mature larva, the thick 5mm of the stem of final section, and the total length of stem is at 45-55cm, and it is longer to choose single-unit, not wounded in the battle stem.
The cloche back cut diameter of the device of pupating of the present invention is 5-13cm, and lower port diameter is 10-25cm, and the top height is 2-6cm, and the lower part height is 18-30cm; The bottom surface diameter on chassis is 15-24cm, and height is 1.5-3.5cm; The bottom surface diameter of vial is 4-9cm, and back cut diameter is 3-7cm, and height is 8-17cm.
The cloche back cut diameter of the device of pupating of the present invention is 9cm, and lower port diameter is 18.5cm, and the top height is 4cm, and the lower part height is 24cm; The bottom surface diameter on chassis is 19.5cm, and height is 2.5cm; The bottom surface diameter of vial is 6.5cm, and back cut diameter is 5cm, and height is 12.5cm.
The bottom surface diameter of vial I of the present invention is 6-10cm, back cut diameter 4-8cm, high 10-13cm; The bottom surface diameter of described vial II is 6-10cm, back cut diameter 4-8cm, high 5.5-9.5cm.
The bottom surface diameter of vial I of the present invention is 8cm, back cut diameter 6cm, high 11.7cm; The bottom surface diameter of described vial II is 8cm, back cut diameter 6cm, high 7.5cm.
The advantage of the straight chest flea beetle experimental population raising method of lotus grass of the present invention is as follows:
(1) cultivates the experimental population of the straight chest flea beetle of lotus grass in neat, consistent, different worm attitudes of a large amount of (or controlled quantity), different worm age for the science scrutiny.
(2) the straight chest flea beetle of lotus grass is the good natural enemy insect of generally acknowledging both at home and abroad, life cycle is short, breeding amount is big, it is normal generation overlap, the problems such as the worm attitude is irregular, quantity is uncontrollable, the more difficult investigation of genetic background found in reproductive process, cause its scientific research bottleneck to occur, and using value is obstructed.Have not yet to see the report that a large amount of artificial feeding experimental populations are arranged both at home and abroad, the present invention will be that scientific research solves the required rigorous material of experiment, cultivates the experimental insect of the straight chest flea beetle of lotus grass in neat, consistent, different worm attitudes of a large amount of (or controlled quantity), different worm age for the science scrutiny.
(3) upper blade and the tender stem of the straight chest flea beetle imago feeding Alternanthera philoxeroides of lotus grass, and ovum produced on blade, but blade withered and yellow rotting easily causes product ovum thereon also along with rotting.The present invention collects pieces of an egg one time day by day at adult eclosion after 3 days, be same batch pieces of an egg, adult and ovum are separated, both guaranteed the survival rate of ovum, again can be so that the brooding time basically identical of ovum be conducive to obtain the more neat straight chest flea beetle experimental population of lotus grass in the length of time.
(4) pupating is the key link that the straight chest flea beetle of lotus grass is finished the history of life and effective control Alternanthera philoxeroides, and mature larva bores moth Alternanthera philoxeroides stem stalk and pupates, but the stem stalk of being stung by larva is easy to rot, and makes the interior pupa of stem stalk also rot.The mature larva that will be about to pupate among the present invention is put in the homemade device of pupating, and uses 8 comparatively sturdy joint Alternanthera philoxeroides stem stalks to pupate for it, has improved the survival rate of pupa greatly.
(5) rearing method of the straight chest flea beetle experimental population of lotus grass of the present invention, method is simple and easy, easily grasp, particularly be convenient to indoor feeding and observe its history of life, even the keeper does not have relevant professional knowledge, also can learn in a short time, the device of pupating can be placed on the foster worm frame of layer-stepping, it is less to take up space, and feeding cost is lower; The straight chest flea beetle of lotus grass can at any time, everywhere, whatever you like be produced, for experimental study provides sufficient worm source.
(6) method of the present invention can realize the uninterruptedly breeding all the year round of this worm, and can be experiment provides single strain and can be used as its material that makes a variation from generation to generation of detection, and needs round in real time, in right amount the test worm source that homogeneous causes according to research institute.
Description of drawings
Fig. 1 is the schematic diagram of vial I of the present invention;
Fig. 2 is the schematic diagram of vial II of the present invention;
Fig. 3 is the pupate structural representation of device of the present invention.
Among the figure: 1, gauze 2, cloche 3, hardboard 4, Alternanthera philoxeroides 5, vial 6, chassis
Following embodiment all can realize the effect of above-mentioned experimental example.
Embodiment
The raising method of the straight chest flea beetle experimental population of a kind of lotus grass, this method is following steps:
(1) one of the filter paper of size at the bottom of putting among the vial I (the bottom surface diameter is 8cm, back cut diameter 6cm, high 11.7cm) bottle soaks with running water; Get the adult of firm emergence and put into bottle, every bottle a worm number is the 20-30 head, get 3 and contain 6 fresh Alternanthera philoxeroides cauline leafs from top, and with putting into vial I behind the running water immersion 10min, gauze is covered on the vial I mouth, fix with rubber band, being placed on temperature is 26 ℃, illumination and dark duration be than being 14h: 10h, and humidity is in 85% the artificial incubator; Early take out vial I before 9 every day in artificial incubator, clamp the stem of residue cauline leaf in the bottle with the tip tweezers, adult is dialled gently or shake off to vial with wolf cub hair pen, getting 3 contains 6 fresh Alternanthera philoxeroides cauline leafs and changes from top, and with putting into vial I behind the running water immersion 10min, seal then;
(2) adult that has just sprouted wings began to lay eggs after the 3rd day, every 24 hours with vial I in pieces of an egg and adult separately, the pieces of an egg of Shou Jiing are same batch each time, label to record at the bottle wall and lay eggs the date; Therefrom take out the cauline leaf that has pieces of an egg from vial I, will have the blade of pieces of an egg to take, (the bottom surface diameter is 8cm, back cut diameter 6cm, high 7.5cm to put into vial II.), be covered with the filter paper that soaks with end size at the bottom of the vial II, put 10 pieces of an egg for every bottle, cut out a preservative film, cover bottleneck, with 10 pin holes of pinprick, add a cover a gauze at the middle part again, wale bottleneck with rubber band; In time spray running water every day in bottle, moistening whole filter paper keeps the humidity in the bottle; Pieces of an egg were hatching in 3-4 days;
(3) the 1st day after the larva hatching directly adds fresh Alternanthera philoxeroides in vial II; Only remove the old leaf with chorion on the 2nd day, and in vial II, add fresh Alternanthera philoxeroides again; Withered Alternanthera philoxeroides blade pressed from both sides out in the 3rd, and with little writing brush remaining few larva on the old blade of Alternanthera philoxeroides is swept down lightly, the fresher Alternanthera philoxeroides of adding in the vial II; Add fresh Alternanthera philoxeroides so repeatedly, 2 ages and 3 instar larvaes can directly be swept down larva with wolf cub hair pen or clamp old grass blade with tweezers and shake off larva down in vial II, add fresh Alternanthera philoxeroides again in vial II; The Alternanthera philoxeroides cauline leaf of handling out is dropped into designated treatment point and destroys;
(4) the mature larva action of pupating in advance is slower, and food ingestion also reduces gradually, and mature larva is inserted in the device of pupating, and the described device of pupating is made up of cloche 2, hardboard 3, Alternanthera philoxeroides 4, vial 5 and the chassis 6 of gauze 1, " protruding " font; Hardboard 3 is cut into " recessed " word shape, it is covered on vial 5, with wide adhesive tape it is wrapped on the vial 5 and fixes, in vial 5, add 9/10 running water; Pupate cloche 2 back cut diameters of device are 9cm, and lower port diameter is 18.5cm, and the top height is 4cm, and the lower part height is 24cm; The bottom surface diameter on chassis 6 is 19.5cm, and height is 2.5cm; The bottom surface diameter of vial 5 is 6.5cm, and back cut diameter is 5cm, and height is 12.5cm.From the greenhouse, get 10 fresh Alternanthera philoxeroides cauline leafs 4 (for mature larva pupate usefulness Alternanthera philoxeroides stem stalk for from top begin the number totally 8 the joint, the thick 5mm of the stem of final section, the total length of stem is in the 45-55cm scope, and it is longer to choose single-unit, not wounded in the battle stem.), the leaf that most end 2 is held successively removes, and the 1cm place otch of a 2cm of cutting sth. askew snarls not make it loose with rubber band outside putting successively apart from last, and the stem that fixes is inserted in the vial 5; Vial 5 is put into chassis 6, an end of Alternanthera philoxeroides 4 cauline leafs is arranged outwardly; Crooked Alternanthera philoxeroides 4 cauline leafs make the top of each stem contact with chassis 6, cover upper glass cover 2; Gauze 1 on cloche 2 main header casings is fixed with rubber band; Cloche 2 outer walls middle part is labelled, label substance is for inserting mature larva date and a worm number, with wolf cub hair pen mature larva is received on Alternanthera philoxeroides 4 cauline leafs lightly, larva is connected on the cane, insert 100 mature larvas in each device of pupating; Gauze 1 on cloche 2 main header casings, fixing with rubber band 7; Cloche 2 outer walls middle part is labelled, and label substance is for inserting mature larva date and a worm number, and mature larva pierces in the Alternanthera philoxeroides 4 stem stalks pupates.It is 26 ℃ that this device of pupating is placed temperature, and illumination and dark duration are than being 14h: 10h, and humidity is that 85% artificial incubator is cultivated; Count the adult that emergence was arranged on the 7th day from the access mature larva and occur, and adult all sprouts wings in ensuing 4 days, just the cane in the device can be taken out at the 11st day, the pupation rate of mature larva can reach 80%-95%.
Claims (20)
1. the raising method of the straight chest flea beetle experimental population of lotus grass is characterized in that the step of this raising method is as follows:
(1) in the vial I, put into bottle at the bottom of one of the filter paper of size, running water soaks; Get the adult of firm emergence and put into bottle, every bottle a worm number is the 15-40 head, get the 2-4 root from the fresh Alternanthera philoxeroides cauline leaf of the top 4-8 of containing sheet, and with putting into the vial I behind the running water immersion 5-15min, gauze is covered on the vial I mouth, fix with rubber band, be placed in the artificial incubator and cultivate, the temperature of artificial incubator is 20-30 ℃, and illumination and dark duration are than being 10-18h:8-12h, and humidity is 70%-90%; Early take out the vial I before 9 every day in artificial incubator, clamp the stem of residue cauline leaf in the bottle with the tip tweezers, dial gently or shake off adult to the vial I with wolf cub hair pen, getting the 2-4 root changes from the fresh Alternanthera philoxeroides cauline leaf of the top 4-8 of containing sheet, and with putting into the vial I behind the running water immersion 5-15min, seal then;
(2) begin to lay eggs after the adult 2-4 day of just having sprouted wings, every 18-30 hour with the vial I in pieces of an egg and adult separately, the pieces of an egg of Shou Jiing are same batch each time, label to record at the bottle wall and lay eggs the date; From the vial I, take out the Alternanthera philoxeroides cauline leaf that has pieces of an egg, to there be the blade of pieces of an egg to take, put into the vial II, be covered with the filter paper that soaks with end size at the bottom of the vial II, put 8-12 sheet pieces of an egg for every bottle, cover bottleneck with preservative film, and sting 10-15 pin hole thereon, add a cover a gauze again, wale bottleneck with rubber band; In time spray running water every day in the vial II, moistening whole filter paper keeps the humidity in the vial II; Pieces of an egg were hatched in 4-6 days;
(3) the 1st day after the larva hatching directly adds fresh Alternanthera philoxeroides in the vial II; Only remove the old leaf with chorion on the 2nd day, and in the vial II, add fresh Alternanthera philoxeroides again; Withered Alternanthera philoxeroides blade pressed from both sides out in the 3rd day, and with little writing brush remaining few larva on the old blade of Alternanthera philoxeroides is swept down lightly, the fresher Alternanthera philoxeroides of adding in the vial II; Add fresh Alternanthera philoxeroides so repeatedly, 2 ages and 3 instar larvaes can directly be swept down larva with wolf cub hair pen or clamp old grass blade with tweezers and shake off larva down in the vial II, add fresh Alternanthera philoxeroides again in the vial II; The Alternanthera philoxeroides cauline leaf of handling out is dropped into designated treatment point and destroys;
(4) the mature larva action of pupating in advance is slower, food ingestion also reduces gradually, and mature larva is inserted in the device of pupating, and with wolf cub hair pen mature larva is received on the Alternanthera philoxeroides cauline leaf lightly, connect larva as far as possible on cane, insert 80-120 mature larva in each device of pupating; Mature larva pierces in the Alternanthera philoxeroides stem stalk pupates, and it is 20-30 ℃ that the device of will pupating is placed on temperature, and illumination and dark duration be than being 10-18h:8-12h, and humidity is to cultivate in the artificial incubator of 70%-90%; Count the adult that emergence was just arranged on the 7th day from the access mature larva and occur, and all sprouted wings in ensuing 4 days, just the cane in the device can be taken out at the 11st day, the mature larva pupation rate can reach 80%-95%.
2. raising method as claimed in claim 1 is characterized in that, the step of this raising method is as follows:
(1) in the vial I, put into bottle at the bottom of one of the filter paper of size, soak with running water; Get the adult of firm emergence and put into bottle, every bottle a worm number is the 20-30 head, get 3 and contain 6 fresh Alternanthera philoxeroides cauline leafs from top, and with putting into the vial I behind the running water immersion 10min, gauze is covered on the vial I mouth, fix with rubber band, be placed in the artificial incubator, the temperature of artificial incubator is 26 ℃, and illumination and dark duration are than being 14h:10h, and humidity is 85%; Early take out the vial I before 9 every day in artificial incubator, clamp the stem of residue cauline leaf in the bottle with the tip tweezers, dial gently or shake off adult to the vial I with wolf cub hair pen, get 3 from the top cauline leaf replacing that contains 6 fresh Alternanthera philoxeroides, and with putting into the vial I behind the running water immersion 10min, seal then;
(2) adult that has just sprouted wings began to lay eggs on the 3rd, every 24 hours with the vial I in pieces of an egg and adult separately, each pieces of an egg of collecting are same batch, lay eggs the date at the bottle wall record of labelling; From the vial I, take out the cauline leaf that has pieces of an egg, to there be the blade of pieces of an egg to take, put into the vial II, be covered with the filter paper that soaks with end size at the bottom of the vial II, put 10 pieces of an egg for every bottle, cover with preservative film, and sting 10 pin holes thereon, add a cover a common gauze again, wale bottleneck with rubber band; In time spray running water every day in bottle, moistening whole filter paper keeps the humidity in the bottle; Pieces of an egg were hatching in 3-4 days;
(3) the 1st day after the larva hatching directly adds fresh Alternanthera philoxeroides in the vial II; Only remove the old leaf with chorion on the 2nd day, and in the vial II, add fresh Alternanthera philoxeroides again; Withered Alternanthera philoxeroides blade pressed from both sides out in the 3rd day, and with little writing brush remaining few larva on the old blade of Alternanthera philoxeroides is swept down lightly, the fresher Alternanthera philoxeroides of adding in the vial II; Add fresh Alternanthera philoxeroides so repeatedly, 2 ages and 3 instar larvaes can directly be swept down larva with wolf cub hair pen or clamp old grass blade with tweezers and shake off larva down in the vial II, add fresh Alternanthera philoxeroides again in the vial II; The Alternanthera philoxeroides cauline leaf of handling out is dropped into designated treatment point and destroys;
(4) the mature larva action of pupating in advance is slower, food ingestion also reduces gradually, and mature larva is inserted in the device of pupating, and with wolf cub hair pen mature larva is received on the Alternanthera philoxeroides cauline leaf lightly, connect larva as far as possible on cane, insert 100 mature larvas in each device of pupating; Mature larva pierces in the Alternanthera philoxeroides stem stalk pupates, and it is 26 ℃ that the device of will pupating is placed on temperature, and illumination and dark duration be than being 14h:10h, and humidity is to cultivate in 85% the artificial incubator; Count the adult that emergence was just arranged on the 7th day from the access mature larva and occur, and adult all sprouts wings in ensuing 4 days, just the cane in the device can be taken out at the 11st day, the mature larva pupation rate can reach 80%-95%.
3. raising method as claimed in claim 1 or 2 is characterized in that the described device of pupating is made up of the cloche (2) of gauze (1), " protruding " font, hardboard (3), Alternanthera philoxeroides (4), vial (5) and chassis (6); Hardboard (3) is cut into " recessed " word shape, it is covered on vial (5), with wide adhesive tape it is wrapped in vial (5) and upward fixes, in vial (5), add 9/10 running water; From the greenhouse, get the fresh Alternanthera philoxeroides stem (4) of 8-12 root, the leaf that most end 1-3 is held successively removes, the 0.5-1.5cm place otch of a 1-3cm of cutting sth. askew snarls not make it loose with rubber band outside putting successively apart from last, and the stem that fixes is inserted in the vial (5); Vial (5) is put into chassis (6), and an end of yerbadetajo seed grass blade in the family way (4) outwardly; Crooked Alternanthera philoxeroides cauline leaf (4) makes the top of each stem contact with chassis (6), cover upper glass cover (2); Gauze (1) on cloche (2) main header casing is fixed with rubber band; Cloche (2) outer wall middle part is labelled, and label substance is for inserting mature larva date and a worm number, if the larva that connect in a day more than or equal to two jars, is then distinguished with A, B, C....
4. raising method as claimed in claim 3 is characterized in that the described device of pupating is made up of the cloche (2) of gauze (1), " protruding " font, hardboard (3), Alternanthera philoxeroides (4), vial (5) and chassis (6); Hardboard (3) is cut into " recessed " word shape, it is covered on vial (5), with wide adhesive tape it is wrapped in vial (5) and upward fixes, in vial (5), add 9/10 running water; From the greenhouse, get 10 fresh Alternanthera philoxeroides stems (4), the leaf that most end 2 is held successively removes, the 1cm place otch of a 2cm of cutting sth. askew snarls not make it loose with rubber band outside putting successively apart from last, and the stem that fixes is inserted in the vial (5); Vial (5) is put into chassis (6), and an end of yerbadetajo seed grass blade in the family way (4) outwardly; Crooked Alternanthera philoxeroides cauline leaf (4) makes the top of each stem contact with chassis (6), cover upper glass cover (2); Gauze (1) on cloche (2) main header casing is fixed with rubber band; Cloche (2) outer wall middle part is labelled, and label substance is for inserting mature larva date and a worm number, if the larva that connect in a day more than or equal to two jars, is then distinguished with A, B, C....
5. as claim 1, one of 2 or 4 described raising method, it is characterized in that described (4) step is in the raising stage of pupa, pupate the Alternanthera philoxeroides stem stalk of usefulness for from the top altogether 6-10 joint of number that begins for mature larva, the thick 4-6mm of the stem of final section, the total length of stem is at 40-60cm, it is longer to choose single-unit, not wounded in the battle stem.
6. raising method as claimed in claim 3, it is characterized in that described (4) step is in the raising stage of pupa, pupate the Alternanthera philoxeroides stem stalk of usefulness for from the top altogether 6-10 joint of number that begins for mature larva, the thick 4-6mm of the stem of final section, the total length of stem is at 40-60cm, it is longer to choose single-unit, not wounded in the battle stem.
7. raising method as claimed in claim 5, it is characterized in that described (4) step is in the raising stage of pupa, for mature larva pupate usefulness Alternanthera philoxeroides stem stalk for from top begin the number totally 8 the joint, the thick 5mm of the stem of final section, the total length of stem is at 45-55cm, it is longer to choose single-unit, not wounded in the battle stem.
8. raising method as claimed in claim 6, it is characterized in that described (4) step is in the raising stage of pupa, for mature larva pupate usefulness Alternanthera philoxeroides stem stalk for from top begin the number totally 8 the joint, the thick 5mm of the stem of final section, the total length of stem is at 45-55cm, it is longer to choose single-unit, not wounded in the battle stem.
9. as claim 4,6, one of 7 or 8 described raising method, the cloche back cut diameter that it is characterized in that the described device of pupating is 5-13cm, and lower port diameter is 10-25cm, and the top height is 2-6cm, and the lower part height is 18-30cm; The bottom surface diameter on chassis is 15-24cm, and height is 1.5-3.5cm; The bottom surface diameter of vial is 4-9cm, and back cut diameter is 3-7cm, and height is 8-17cm.
10. raising method as claimed in claim 3, the cloche back cut diameter that it is characterized in that the described device of pupating is 5-13cm, and lower port diameter is 10-25cm, and the top height is 2-6cm, and the lower part height is 18-30cm; The bottom surface diameter on chassis is 15-24cm, and height is 1.5-3.5cm; The bottom surface diameter of vial is 4-9cm, and back cut diameter is 3-7cm, and height is 8-17cm.
11. raising method as claimed in claim 5, the cloche back cut diameter that it is characterized in that the described device of pupating is 5-13cm, and lower port diameter is 10-25cm, and the top height is 2-6cm, and the lower part height is 18-30cm; The bottom surface diameter on chassis is 15-24cm, and height is 1.5-3.5cm; The bottom surface diameter of vial is 4-9cm, and back cut diameter is 3-7cm, and height is 8-17cm.
12. raising method as claimed in claim 9, the cloche back cut diameter that it is characterized in that the described device of pupating is 9cm, and lower port diameter is 18.5cm, and the top height is 4cm, and the lower part height is 24cm; The bottom surface diameter on chassis is 19.5cm, and height is 2.5cm; The bottom surface diameter of vial is 6.5cm, and back cut diameter is 5cm, and height is 12.5cm.
13. as claim 10 or 11 described raising method, the cloche back cut diameter that it is characterized in that the described device of pupating is 9cm, lower port diameter is 18.5cm, and the top height is 4cm, and the lower part height is 24cm; The bottom surface diameter on chassis is 19.5cm, and height is 2.5cm; The bottom surface diameter of vial is 6.5cm, and back cut diameter is 5cm, and height is 12.5cm.
14. as claim 1,2,4,6,7,8, one of 10 or 11 described raising method, the bottom surface diameter that it is characterized in that described vial I is 6-10cm, back cut diameter 4-8cm, high 10-13cm; The bottom surface diameter of described vial II is 6-10cm, back cut diameter 4-8cm, high 5.5-9.5cm.
15. raising method as claimed in claim 3, the bottom surface diameter that it is characterized in that described vial I is 6-10cm, back cut diameter 4-8cm, high 10-13cm; The bottom surface diameter of described vial II is 6-10cm, back cut diameter 4-8cm, high 5.5-9.5cm.
16. raising method as claimed in claim 5, the bottom surface diameter that it is characterized in that described vial I is 6-10cm, back cut diameter 4-8cm, high 10-13cm; The bottom surface diameter of described vial II is 6-10cm, back cut diameter 4-8cm, high 5.5-9.5cm.
17. raising method as claimed in claim 9, the bottom surface diameter that it is characterized in that described vial I is 6-10cm, back cut diameter 4-8cm, high 10-13cm; The bottom surface diameter of described vial II is 6-10cm, back cut diameter 4-8cm, high 5.5-9.5cm.
18. raising method as claimed in claim 13, the bottom surface diameter that it is characterized in that described vial I is 6-10cm, back cut diameter 4-8cm, high 10-13cm; The bottom surface diameter of described vial II is 6-10cm, back cut diameter 4-8cm, high 5.5-9.5cm.
19. raising method as claimed in claim 14, the bottom surface diameter that it is characterized in that described vial I is 8cm, back cut diameter 6cm, high 11.7cm; The bottom surface diameter of described vial II is 8cm, back cut diameter 6cm, high 7.5cm.
20. as the described raising method of one of claim 15-18, the bottom surface diameter that it is characterized in that described vial I is 8cm, back cut diameter 6cm, high 11.7cm; The bottom surface diameter of described vial II is 8cm, back cut diameter 6cm, high 7.5cm.
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| CN103960200B (en) * | 2014-04-23 | 2016-05-18 | 长江大学 | The rearing method of a kind of phyllotreta striolata adult |
| CN104604814B (en) * | 2015-02-17 | 2017-01-25 | 浙江省农业科学院 | Method for efficient chilo suppressalis breeding |
| CN104756956B (en) * | 2015-03-26 | 2017-08-25 | 宜昌市农业生态与资源保护站 | Plant modification Agasicles hygrophila facility is built |
| CN109122589B (en) * | 2018-08-22 | 2020-10-27 | 山西农业大学 | Lotus herb flea beetle stem externalization pupation device based on humidity control and experiment method |
| CN111280165B (en) * | 2020-03-04 | 2021-10-29 | 山西农业大学 | Low-temperature preservation method for agasicles hygrophila eggs |
| CN113348922A (en) * | 2021-05-07 | 2021-09-07 | 江苏大学 | Method for controlling alternanthera philoxeroides by using facultative insects and/or special feeding insects |
| CN114403098A (en) * | 2021-12-22 | 2022-04-29 | 中国农业科学院农业基因组研究所 | Lotus herb direct chest flea beetle pupating device and pupating method thereof |
| CN118140877B (en) * | 2024-05-11 | 2024-07-12 | 云南千淇生物科技有限公司 | Breeding device and breeding method for breeding Lepidius crassipes |
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| HU223567B1 (en) * | 2002-06-25 | 2004-09-28 | Gabonatermesztési Kutató Kht. | Gk ati variety of early eating wheat (triticum aestivum l.) |
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