CN101940193B - Method for indoor propagation and outdoor release of cybocephalus - Google Patents
Method for indoor propagation and outdoor release of cybocephalus Download PDFInfo
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- CN101940193B CN101940193B CN2010102883152A CN201010288315A CN101940193B CN 101940193 B CN101940193 B CN 101940193B CN 2010102883152 A CN2010102883152 A CN 2010102883152A CN 201010288315 A CN201010288315 A CN 201010288315A CN 101940193 B CN101940193 B CN 101940193B
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Abstract
The invention belongs to the technical field of biologically controlling agricultural and forestry pests, and in particular relates to a method for the indoor propagation and the outdoor release of natural enemy insects. The method comprises the following steps of: using potatoes as a propagation material of an intermediate host; using oriental kidney armored scales which are easy to mass propagation indoors and have wide humiture adaptation range as the intermediate host, putting the intermediate host cultured for about 20-30 days in a disposable cup with a lid, inoculating and propagating a cybocephalus seed source in a sex ratio of 1 to 1, taking out the cybocephalus seed source after 10-15 days and transferring to another cup until the seed source is dead; putting the rest potatoes in rows in a plastic box with bilayer gauze on the bottom layer to collect pupas; and putting the collected pupas in an adult insect collecting cage or releasing the pupas in the field so as to prevent armored scale pests or releasing the collected adult insects in the field. The invention can carry out the mass production on the cybocephalus and solve the problems of low propagation efficiency and annual productivity incapability of the cybocephalus.
Description
Technical field
The invention belongs to agriculture and forestry injurious insect biological control technical field, relate to the indoor of a kind of square toes first and breed and open-air method for releasing.
Background technology
Diaspididae is a most species in the Homoptera Sternorrhyncha, and the monoid of evolving most is distributed widely in the whole world, and You Yiya, Europe, non-continent are the abundantest.Diaspididae has write down 2088 kinds of 372 genus at present; Account for 1/3rd of armored scale class caste; And the known about kind more than 320 of armored scale kind of China, main parasitic is the important pests monoid of forestry, fruit tree, economic crops and ornamental plants in various arbors, shrub and herbaceous plant.Because the special morphosis of armored scale class pest, chemical control is difficulty very, and the economic loss that causes is unable to estimate.Armored scale worm kind natural enemy aboundresources; The square toes first is most important predator monoid, and its host is extensive, can adapt to various habitats; The predation rate is high; Both can be used for the biological control of the armored scale in the forestry, also can be used for the armored scale class pest of fruit tree, economic crops and ornamental plants, and stung western a red-spotted lizard, brown round a red-spotted lizard, citrus like predation arrowhead scales, chaff sheet a red-spotted lizard, chaff scale, pawpaw and take turns a red-spotted lizard, Milan in vain and take turns a red-spotted lizard, camphor tree capsule a red-spotted lizard, Pseudaulacaspis pentagona, persimmon suede mealybug, tangerine Panonychus citri etc. in vain; The breeding value is arranged very much, have a extensive future.
Square toes first (Scale picnic beetle), the Insecta coleoptera.This section has recorded and narrated nearly 140 kinds.The sort research that (comprises African Realm and belong to palearctic African northern territory) square toes first section in Africa is comparatively deep.The square toes first kind of having recorded and narrated in the world, the kind more than 2/3 is distributed in Africa, kind surplus the kind of Palaearctic region square toes first has recorded and narrated 40, India and subprovince, Malaysia square toes first kind only have fragmentary description and record, and the thumping majority kind all belongs to square toes first genus in the kind.The square toes first research of China is deep not enough, up to the present only puts down in writing 9 kinds.
People such as Tian Mingyi, the square toes first belongs to Chinese kind description and a novel species is recorded and narrated.The insect natural enemy, 16 (3), 1994.This article the other side helmet belongs to Chinese kind and has carried out the form description, and has recorded and narrated the square toes first of picking up from Nan Ling and belong to novel species.
1, Japanese square toes first (Cybocephalus nipponicus Endrody Yonnga), body long (not comprising curved down head) 1.00～1.35mm, wide 0.75～0.95mm.
2, little bright square toes first (C.sublucidus Plavilshtshikov), body long (not comprising curved down head) 2.10mm, wide 1.30mm.
3, southern mountain range square toes first (Cybocephalus nanlingensissp.Nov), body long (not comprising curved down head) 1.18～1.20mm, wide 0.97～0.10mm.
People such as Tian Mingyi, Hainan Province's square toes first belongs to kind and records and narrates.Agricultural University Of South China's journal, 18 (1), 1997.This article author investigates Hainan Province's square toes first kind, identifies the square toes first altogether and belongs to 8 kinds of kinds, that is:
1, Japanese square toes first (Cybocephalus nipponicus Endrody-Yonnga, 1971), body is long (not to comprise curved down head; 1.00～1.35mm down together), wide 0.76～0.95mm, male worm head and pronotary are yellow; Foot is yellow, pronotary lateral margin and elytrum ora terminalis yellowish-brown, remainder black; Female polypide black distributes: Hainan, Liaoning, Beijing, Shaanxi, Zhejiang, Jiangxi, Hunan, Hubei, Shanxi, Fujian, Guangdong, Guangxi, Sichuan, Guizhou, Yunnan, Hong Kong; Be distributed in Japan, India, Sri Lanka, Mariana Islands, Micronesia abroad.
2, Pang Shi square toes first (Cybocephaluspangi Yu Tian, 1995), the wide 0.70～0.90mm of the long 0.90～1.20mm of body, similar than the small body type kind with Japanese square toes first, distribute: Hainan, Guizhou.
3, drastic crack square toes first (Cybocephalus dissectus Yu, 1994), individual difference in size is bigger. the long 1.25～1.80mm of body, and wide 0.80～1.20mm, the black kind, the extremely horizontal arch of body distributes: Hainan, Guangdong, Sichuan, Guangxi.
4, recessed stem square toes first (Cybocephalus concavus Yu et Tian, 1995), the long 1.10～1.30mm of body, wide 0.8mm.Body black distributes: Hainan, Guangdong.
5, swollen joint square toes first (Cybocephalus exlansus Yu, 1994), different name: southern mountain range square toes first (Cybocephalus nanlingensis Tnan, 1994), the long 1.10～1.40mm of body, body black distributes: Hainan, Guangdong.
6, Chinese square toes first (Cybocephalus chinwnsis YuCybocephalus, 1995), the long 1.2～1.5mm of body, wide 0.8mm, body black distributes: Hainan, Zhejiang, Guangdong, Guangxi, Sichuan, Guizhou.
7, Mashan square toes first (Cybocephalus mashanus Yu, 1994), the long 1.08～1.22mm of body, wide 0.90～1.00mm, body black, feeler 11 joints distribute: Hainan, Guangdong, Guangxi.
8, Guilin square toes first (Cybocephalus guilinensis Yu, 1995), the long 1.00～1.10mm of body, wide 0.8mm, build is less, and body black distributes: Hainan, Guangxi.
People such as Li Hongjun, Japanese square toes first is to the reaction of arrowhead scales predation under the different condition.The plant protection journal, 36 (6), 2009.Under indoor conditions; Quantitative study under different temperatures, humidity and the illumination condition Japanese square toes first adult to 1 age of different densities arrowhead scales male nymph the predation reaction and seek effect; The result shows; Temperature is that 25 ℃, relative moisture are 70～90%, intensity of illumination 12000lx, L: D=14:, help Japanese square toes first adult predation most at 10 o'clock.Theoretical maximum predation amount is 308.86, and best searching density is 95.18.
People such as Ran Chun, the influence that temperature is grown and bred Japanese square toes first.The plant protection journal, 30 (4), 2003.This article is according to the correlation of temperature and the change of Japanese square toes first experimental population; The development duration of each worm attitude of match, survival rate and adult egg laying amount and model between temperature; Fundamental reaction the temperature variant trend of population each item ecological characteristic, can supply indoor mass rearing to breed Japanese square toes first reference is provided.Research and inquirement Japanese square toes first is to the reaction of temperature in 18～30 ℃ of scopes, and this worm is to the reaction of extreme temperature undetermined still.
Find out from the mensuration result of Wang Shi model; Ovum, first-instar young, second instar larvae, third-instar larvae, pupa and the preoviposition period the critical developmental temperature of high temperature is respectively 37.1,36.2,35.3,41.3,45.1,43.1 ℃; Explain that Japanese square toes first is more high temperature resistant; Whether this conforms to actual conditions, remains further to be studied.
Adopt the way of low temperature storage Japan square toes first cocoon and ovum can postpone the time that adult eclosion and ovum are hatched, for planned indoor extensive raising with discharge Japanese square toes first pest control and provide convenience.When being housed in 8 ℃, the eclosion rate of Japanese square toes first pupa and the incubation rate of ovum are the highest.So 8 ℃ is low temperature storage Japan's square toes first pupa and the more satisfactory temperature of ovum; And the Wang Shi model simulates the critical developmental temperature of the low temperature of each worm attitude between 3.5～6.2 ℃; Two groups of data are more approaching, so the critical developmental temperature of low temperature can be used as the reference frame that indoor low temperature is preserved Japanese square toes first.
Japan square toes first adult can only preserve in low temperature in 15 days, and lethality sharply increases after 15 days, so should not carry out low tempertaure storage with Japanese square toes first adult.
People such as Ran Chun, the method for the Japanese square toes first of a kind of indoor feeding.The plant doctor, 16 (6), 2003.This article has been introduced the method to Japanese square toes first indoor feeding:
1, the host of the raising white mulberry scale of white mulberry scale is more, like Ailanthus altissima, mulberry tree, peach, Japanese plum etc., be easy to adopt.Treat that white mulberry scale gets into the hatching Sheng phase of laying eggs, choose with dissecting needle and be split into worm a red-spotted lizard shell, with ovum with just incubate nymph and move in the surface plate, fetch indoor inoculation to potato, let its hatching, growth, growth and breeding with No. 0 writing brush.Can adopting later on uses the same method obtains the worm source on indoor potato.Be inoculated on other nonvaccinated potatos, further enlarge the white mulberry scale captive breeding, for the raising of Japanese square toes first provides a large amount of feeds.Indoor relative humidity maintains between 70～80%, and temperature is at 28 ℃, and when every day, illumination was no less than 12h, it is more suitable that white mulberry scale is grown, grows, bred.
2, the raising of Japanese square toes first
White mulberry scale do not grow as yet to adult during this period of time in, should not inoculate Japanese square toes first, otherwise Japanese square toes first is eaten up the white mulberry scale on the potato very soon, this mass rearing to Japanese square toes first is disadvantageous.In order to let the white mulberry scale of equal number raise more Japanese square toes first, it is the most desirable to wait white mulberry scale to grow after the adult stage the Japanese square toes first of inoculation.Japanese square toes first on each potato should not be inoculated too much, otherwise also can eat up white mulberry scale at short notice, and white mulberry scale and Japanese square toes first are proper in 50: 1 ratio inoculation.Treat Japanese square toes first after the growth of laying eggs on the potato, the offspring that the review dates we helmet produces, in generation, is discharged into the field and controls armored scale insects such as tangerine Panonychus citri and arrowhead scales thereafter on demand, or carries out low tempertaure storage, for future use.The indoor suitable raising temperature of Japan's square toes first is 23.2～27.1 ℃.
3, the low temperature storage of Japanese square toes first
The Japanese square toes first of indoor feeding not necessarily is released into the field at once, must preserve some times in order to need.Low temperature can delay the g and D progress of insect, can accumulate the provenance of Japanese square toes first, supplies breeding in good time and field to discharge the harm that is used for pest control.Low tempertaure storage Japan's square toes first ovum and pupa are the effective ways of preserving Japanese square toes first.Under identical holding conditions, 8 ℃ is the optimum low temperature storage temperature of pupa and ovum.Japan's square toes first ovum, pupa and adult place 2 ℃ respectively, and 4 ℃, 6 ℃, 8 ℃, 10 ℃, the egg hatching rate behind the 25d is respectively 72.1%, 76.5%, and 79.4%, 82.7% and 68.7%; The eclosion rate of pupa is respectively 62.3%, 69.5%, 73.5%, 80.7%, 61.1%.And the adult survival rate behind the 15d is respectively 52.1%, 53.1%, 52.7%; 52.9%, 50.3%, during to 15d; Survival rate at each temperature all is not higher than 53.5%; When the 25d survival rate the highest also less than 4.0%, so Japanese square toes first adult should not preserve more than the 15d, thereby can not carry out low temperature long preservation worm kind with the adult of Japanese square toes first in low temperature.
People such as Lei Yanmei, the biological property of Japanese square toes first and utilization thereof.Plant protection, 30 (4), 2004.This paper introduces and in the greenhouse, raises Sang Bai shield ant, san jose scale with pumpkin or potato piece; Growth phase ought be for a long time on pumpkin or potato piece to treat it, can be with having worm pumpkin or potato piece to move in thin wire gauze guard, and inoculation reproduction Japan square toes first; When treating adult eclosion, be released to the orchard.
In sum; The indoor propagation technical research of square toes first only people such as Ran Chun has report, and this research only is in laboratory stage, can not raise on a large scale; There is following problem: the one, the vector of square toes first is selected improper; The reproduction rate of white peach scale on potato is low, causes inoculating the food of getting that coccid when too early has just been satisfied not the square toes first, and the reproductive efficiency of square toes first is also low; The 2nd, it is long that square toes first adult is gone through the phase, and oviposition period is long, causes generation overlap, is unfavorable for open-air releasing research, and this research does not relate to the research of propagation production in batches.And the research of the artificial feed of square toes first also is in laboratory stage; With egg yolk, honey (5: 5) be matrix no matter adding with do not add micro-bright arrowhead scales body fluid or by the manual work of egg yolk+honey+royal jelly (every milliliter contains 40 milliliters of royal jelly) of preparation in 5: 5: 2 for feed, adult all can be got food.The juice of also desirable food corn of adult or sponge gourd fresh flower powder is not got the tealeaves pollen of dried zasiokaurin of food and storage in addition.But after adult fed and raises artificial feed, lethality increased greatly, and can not lay eggs, in case after using hello the female worm feed of arrowhead scales instead, get final product normal spawning, showed with artificial only can earn a bare living for feed.Therefore, solution party's helmet reproductive efficiency low, can not realize the batch process of annual, at first to collect and filter out a kind of can large-scale breeding, adapt to the wide armored scale of temperature humidity range, and the propagating materials that is used to breed armored scale is easy to get; The selection that next is breeding container, connect worm ratio and armored scale length of time makes the egg laying amount maximization of square toes first; Be the simplification of the open-air method for releasing of square toes first at last.
Summary of the invention
The problem that the reproductive efficiency that the objective of the invention is to overcome the square toes first is low, can not realize the batch process of annual provides a kind of the indoor of square toes first that can produce in enormous quantities to breed and open-air method for releasing.
The present invention is achieved in that
Gather earlier square toes first provenance and vector armored scale provenance in the open air, vector armored scale provenance is inoculated on the culture materials surface of sterilization, cultivate under field conditions (factors); After cultivating 20～30 day time, again 1～2 of new culture materials is put into Disposable paper cup with cover, insert each one of square toes first provenance male and female; Carry out the breeding of square toes first, took out in the square toes first provenance immigration another Disposable paper cup with cover in 10～15 days, dead until square toes first provenance; Then culture materials is taken out from cup; Concentrated flat raft is put into the bottom and is placed with two cultivation boxes with the onesize pupa collection material in bottom, treats after 10～15 days that larva all pupates, collection material field release that can the band pupa; Perhaps put into adult and collect cage cultivation square toes first adult, open-air again release.
Above-described vector armored scale provenance is an east kidney armored scale.
Above-described culture materials is a potato.
Above-described natural conditions are that temperature is 15～35 ℃, and relative moisture is 50～80%, and humidity surpasses 80% needs to use the dehumidifier dehumidifier.
Above-described Disposable paper cup with cover, mid portion is cut, and is beneficial to breathe freely.
Above-described pupa collection material is a hospital gauze.
Above-described cultivation box comprises one or more cultivation boxes in plastic casing, plastics gauze box, chemical yarn & fabric box, tub and the carton.
Above-described collection cage; Be made up of aluminium chassis, glass plate, plastics or chemical yarn & fabric, funnel, test tube, tampon, circular hole and black cloth cover, the cage body is positive square or rectangle, and cage body length and width height all is 30～60cm * 30～60cm * 30～60cm; Upper and lower surface is a glass plate; The surrounding is fixed with plastics or chemical yarn & fabric, and the glass on the top is opened a circular hole, the about 4～6cm of diameter; The shape of black cloth cover is similar with the cage body, and is big slightly, and the circular hole of the about 6～10cm of a diameter is also opened at the top, and funnel is stood upside down, and covers circular port on the cage, and interface cements with glue; During use, be put in the pupa collection material in the cage after, be about to the black sheet and be placed on the cage body, funnel exposes through the round hole of black cloth cover, test tube is placed on the funnel osculum, the place of contact seals with tampon, cage is put in light is local preferably to get final product.
Above-described open-air release comprises that pupa discharges and adult discharges.
Specifically breed and open-air method for releasing step is:
(1) collection of square toes first and vector armored scale provenance:
The square toes first of field acquisition and the adult of vector armored scale or larva or ovum or pupa are taken back indoor subsequent use as provenance.
(2) breeding of vector armored scale:
At first potato is cleaned, dried subsequent usely behind the spray disinfectant, the east kidney armored scale nymph that will just hatch is then swept on the surface of potato with writing brush; 180～200 of nymphs on each potato; It is 15～35 ℃ that the potato that will have nymph again places temperature, and relative moisture is under 50～80% the condition, lets its growth; Take out subsequent usely after 20～30 days, stay a part and breed east of future generation kidney armored scale as provenance.
(3) breeding of square toes first:
At first, have the potato that grows 20～30 days east kidney armored scales with 1 and put into Disposable paper cup with cover, insert each one of square toes first provenance male and female, at rim of a cup lid layer 50 purpose gauze, cover lids again.Taking out square toes first provenance after 10～15 days moves in the another Disposable paper cup with cover, at rim of a cup lid layer 50 purpose gauze, cover lids again; Dead until square toes first provenance, then potato is taken out from cup, concentrated flat raft is put into plastic casing or tub or carton; Put into two overlapping hospital gauzes onesize in its bottom before putting into the bottom; Treat in time to take out potato after larva all pupates, at last hospital gauze is put into adult and collect cage, collect adult.
(4) field of square toes first discharges
The release of square toes first has two kinds of methods:
1, pupa discharges: the hospital gauze that will have square toes first pupa directly hangs on the plant that has armored scale, can climb to voluntarily behind the adult eclosion and seek and get the food insect on the plant that is injured.
2, adult discharges: the adult of collecting is put into Disposable paper cup with cover be hung on the plant that is injured, remove bowl cover and gauze, put into some shredded paper bars in the Disposable paper cup, the food insect is sought and gets in the climbing that is beneficial to adult.
Advantage of the inventive method and good effect:
1, can produce in enormous quantities and breed the square toes first, be used to prevent and treat armored scale insect such as pine greedy scale, the white armored scale of Kao Shi, the California red scale of agricultural, forestry and ornamental plant, brown round a red-spotted lizard etc.
2, with the vector of east kidney armored scale as breeding square toes first, the generation of east kidney armored scale is short, and host's wide adaptability can adopt different propagating materialss with suiting measures to local conditions.The present invention is to be propagating materials with the potato, and kidney armored scale survival rate on potato in east is high, egg laying amount is big, is easy to large-scale breeding, and the scale breeding potentiality of square toes first are significantly improved; The temperature humidity range that adapts to is wide, therefore, even also can breed in the winter time.
3, the breeding of square toes first adopts Disposable paper cup to replace large-scale tub, reduces reproductive-cost; Adopt the mode of single butt joint worm breeding, can significantly improve the egg laying amount of square toes first, disposable the laying eggs of 1 the other side's helmet can reach more than 60 at most.
4, adopt the fixing egg-laying time of square toes first promptly promptly to remove the mode of adult in 10 days, can make each individual worm attitude of the next generation neat, reach the purpose of batch process.
5, adopt double gauze can effectively attract square toes first larva to pupate above that, play the effect of collecting pupa.
6, take the mode that pupa is put in the field to replace the open-air adult that discharges, can greatly practice thrift the man power and material.
Description of drawings
Fig. 1: be square toes first large-scale breeding method process chart of the present invention;
Fig. 2: for adult is collected the cage sketch map.
Fig. 2 sign: 1-glass plate, 2-aluminium chassis, 3-plastics or chemical yarn & fabric, 4-black cloth cover, 5-circular hole, 6-test tube, 7-tampon, 8-funnel.
Embodiment
Below the inventive method is done further to describe in detail, but should be appreciated that the present invention is not limited by these contents through embodiment.
Embodiment 1:
(1) collection of square toes first and vector armored scale provenance:
On the plant in receiving the oranges and tangerines orchard of armored scale pest damage, collect ovum or larva or the pupa or the adult of square toes first and vector east kidney armored scale respectively, take back indoor subsequent use as provenance.
(2) breeding of vector:
At first potato is cleaned, spray disinfectant or in disinfectant, clean after dry subsequent usely, the east kidney armored scale nymph that will just hatch is then swept on the potato with writing brush; 180～200 of nymphs on each potato; The potato that will have nymph again places 27～30 ℃ of temperature, under the condition of humidity 65～70%, lets it freely grow; Take out subsequent usely after 20 days, stay a part and breed east of future generation kidney armored scale as provenance.
(3) breeding of square toes first:
At first, have the potato that grows 20 days east kidney armored scales with 1 and put into treated Disposable paper cup with cover, insert each one of square toes first provenance male and female, let it freely get food and lay eggs.The bowl cover of disposable dixie cup with cover need pass through certain processing, is about to mid portion and cuts, and is beneficial to breathe freely.For anti-square toes first is escaped from, should be earlier at rim of a cup lid layer 50 purpose gauze, cover lids again.Take out square toes first provenance with suction pipe after 15 days and move in another Disposable paper cup with cover, dead until square toes first provenance, wherein death can replenish the square toes first adult of a same sex as in the process that moves, finding.
Then, potato is taken out from cup, put into big plastic casing with concentrating tiling, size is not limit, and potato can not overlappingly be placed.Before putting on the container bottom pad two hospital gauzes onesize with the bottom, treat that the larva back of all in gauze, pupating in time takes out potato, the pollution gauze in case potato is rotted.
At last, the gauze of band pupa is put into the adult gatherer.
(4) field of square toes first discharges
The gauze that will have square toes first pupa hangs on the plant that receives armored scale harm, and the adult of emergence can climb to voluntarily and seek on the plant that is injured and get and eat insect, thereby plays the effect of pest control.
Embodiment 2:
(1) collection of square toes first and vector armored scale provenance:
Receiving on the loose woodsy plant of armored scale pest damage, collecting ovum or larva or the pupa or the adult of square toes first and vector east kidney armored scale respectively, taking back indoor subsequent use as provenance.
(2) breeding of vector:
At first potato is cleaned, spray disinfectant or in disinfectant, clean after dry subsequent usely, the east kidney armored scale nymph that will just hatch is then swept on the potato with writing brush; About 190～210 of nymph on each potato; The potato that will have nymph again places 25～28 ℃ of temperature, under the condition of humidity 72～75%, lets it freely grow; Take out subsequent usely after 25 days, stay a part and breed east of future generation kidney armored scale as provenance;
(3) breeding of square toes first:
At first, have the potato that grows 20 days east kidney armored scales with 1 and put into treated Disposable paper cup with cover, insert each one of square toes first provenance male and female, let it freely get food and lay eggs.The bowl cover of disposable dixie cup with cover need pass through certain processing, is about to mid portion and cuts, and is beneficial to breathe freely.For anti-square toes first is escaped from, should be earlier at rim of a cup lid layer 50 purpose gauze, cover lids again.Take out square toes first provenance with suction pipe after 12 days and move in another Disposable paper cup with cover, dead until square toes first provenance, wherein death can replenish the square toes first adult of a same sex as in the process that moves, finding.
Then, potato is taken out from cup, put into big carton with concentrating tiling, size is not limit, and potato can not overlappingly be placed.Before putting on the container bottom pad two hospital gauzes onesize with the bottom, treat that the larva back of all in gauze, pupating in time takes out potato, the pollution gauze in case potato is rotted.
At last, the gauze of band pupa is put into the adult gatherer, collect adult, open-air release used or as the provenance of square toes first of future generation.
(4) field of square toes first discharges
The adult of collecting is put into Disposable paper cup with cover be hung on the plant that is injured, remove bowl cover and gauze, put into some shredded paper bars in the Disposable paper cup, the food insect is sought and gets in the climbing that is beneficial to adult, thereby plays the effect of pest control.
Embodiment 3:
(1) collection of square toes first and vector armored scale provenance:
On ornamental plant sago cycas that receives the armored scale pest damage or Indian fig plant, collect ovum or larva or the pupa or the adult of square toes first and vector east kidney armored scale respectively, take back indoor subsequent use as provenance.
(2) breeding of vector:
At first potato is cleaned, spray disinfectant or in disinfectant, clean after dry subsequent usely, the east kidney armored scale nymph that will just hatch is then swept on the potato with writing brush; 200 of nymphs on each potato; The potato that will have nymph again places 27～30 ℃ of temperature, under the condition of humidity 76～80%, lets it freely grow; Take out subsequent usely after 28 days, stay a part and breed east of future generation kidney armored scale as provenance;
(3) breeding of square toes first:
At first, have the potato that grows 20 days east kidney armored scales with 1 and put into treated Disposable paper cup with cover, insert each one of square toes first provenance male and female, let it freely get food and lay eggs.The bowl cover of Disposable paper cup with cover need pass through certain processing, is about to mid portion and cuts, and is beneficial to breathe freely.For anti-square toes first is escaped from, should be earlier at rim of a cup lid layer 50 purpose gauze, cover lids again.Take out square toes first provenance with suction pipe after 10 days and move in another Disposable paper cup with cover, dead until square toes first provenance, wherein death can replenish the square toes first adult of a same sex as in the process that moves, finding.
Then, potato is taken out from cup, put into big tub with concentrating tiling, size is not limit, and potato can not overlappingly be placed.Before putting on the container bottom pad two hospital gauzes onesize with the bottom, treat that the larva back of all in gauze, pupating in time takes out potato, the pollution gauze in case potato is rotted.
At last, the gauze of band pupa is put into the adult gatherer, collect adult.
(4) field of square toes first discharges
The adult of collecting is put into Disposable paper cup with cover be hung on the plant that is injured, remove bowl cover and gauze, put into some shredded paper bars in the Disposable paper cup, the food insect is sought and gets in the climbing that is beneficial to adult, thereby plays the effect of pest control.
Claims (3)
1. breed and open-air method for releasing the indoor of a square toes first, it is characterized in that: the vector armored scale is cultivated under field conditions (factors), newer culture materials potato is put into culture vessel; Insert the square toes first; Carry out the breeding of square toes first, pupate, can discharge with the collection material hospital gauze of pupa is open-air; Perhaps put into adult and collect cage cultivation square toes first adult, open-air again release;
Described vector armored scale is cultivated under field conditions (factors), earlier potato is cleaned, and dries subsequent use behind the spray disinfectant; The east kidney armored scale nymph that will just hatch is then swept on the surface of potato with writing brush; 180～210 of nymphs on each potato, it is 15～35 ℃ that the potato that will have nymph again places temperature, relative moisture is to grow under 50～80% the condition; Take out subsequent usely after 20～30 days, stay a part and breed east of future generation kidney armored scale as provenance.
2. breed and open-air method for releasing the indoor of a kind of square toes first according to claim 1, it is characterized in that: described square toes first breeding, and pupating is to have the potato that grows 20～30 days east kidney armored scales with 1 to put into Disposable paper cup with cover; Insert each one of square toes first provenance male and female, at rim of a cup lid layer 50 purpose gauze, cover lids again; Taking out square toes first provenance after 10～15 days moves in the another Disposable paper cup with cover, at rim of a cup lid layer 50 purpose gauze, cover lids again; Dead until square toes first provenance, then potato is taken out from cup, concentrated flat raft is put into plastic casing or tub or carton; Put into two overlapping hospital gauzes onesize in its bottom before putting into the bottom; Treat in time to take out potato after larva all pupates, hospital gauze is put into adult collect cage, collect adult.
3. breed and open-air method for releasing the indoor of a kind of square toes first according to claim 1, it is characterized in that: described collection cage, form by aluminium chassis, glass plate, plastics or chemical yarn & fabric, funnel, test tube, tampon, circular hole and black cloth cover; The cage body is positive square or rectangle; Cage body length and width height is 30～60cm * 30～60cm * 30～60cm, and upper and lower surface is a glass plate, and the surrounding is fixed with plastics or chemical yarn & fabric; Glass on the top is opened a circular hole, diameter 4～6cm; The shape of black cloth cover is similar with the cage body, and is big slightly, and the circular hole of a diameter 6～10cm is also opened at the top, and funnel is stood upside down, and covers circular port on the cage, and interface cements with glue; During use, be put in the pupa collection material in the cage after, be about to the black cloth enclosure on the cage body, funnel exposes through the round hole of black cloth cover, test tube is placed on the funnel osculum, the place of contact seals with tampon, cage is put in light is local preferably to get final product.
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| CN2010102883152A CN101940193B (en) | 2010-09-21 | 2010-09-21 | Method for indoor propagation and outdoor release of cybocephalus |
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| CN2010102883152A CN101940193B (en) | 2010-09-21 | 2010-09-21 | Method for indoor propagation and outdoor release of cybocephalus |
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| CN101595857A (en) * | 2009-05-08 | 2009-12-09 | 中国农业科学院植物保护研究所 | The artificial extensive raising method of diet of ophraella communa |
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| CN101595857A (en) * | 2009-05-08 | 2009-12-09 | 中国农业科学院植物保护研究所 | The artificial extensive raising method of diet of ophraella communa |
Non-Patent Citations (2)
| Title |
|---|
| 冉春等.一种室内饲养日本方头甲的方法.《植物医生》.2003,第16卷(第6期),第38-39页. * |
| 冉春等.温度对日本方头甲生长发育及繁殖的影响.《植物保护学报》.2003,(第4期),第383-388页. * |
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