CN105746435B - A kind of method using rice moth egg breeding chrysopa septempunctata larva - Google Patents
A kind of method using rice moth egg breeding chrysopa septempunctata larva Download PDFInfo
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- CN105746435B CN105746435B CN201610103922.4A CN201610103922A CN105746435B CN 105746435 B CN105746435 B CN 105746435B CN 201610103922 A CN201610103922 A CN 201610103922A CN 105746435 B CN105746435 B CN 105746435B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Abstract
The present invention relates to a kind of method using rice moth egg breeding chrysopa septempunctata larva, including：Obtain rice moth egg and raising two step of chrysopa septempunctata; wherein raise chrysopa septempunctata includes raising adult, the raising of ovum phase, larva free-ranging, pupa time receipts cocoon, pupa time receipts cocoon, the emergence of adult, the refrigeration of product and packaging and other steps again; the present invention from the processing of rice moth egg, the collection of chrysopa septempunctata ovum and storage, the free-ranging of chrysopa septempunctata larva, pupate and receive the key technique of each raisings such as cocoon, development emergence and the refrigeration of different worm state products; research and probe is carried out, to provide scientific basis using rice moth egg biological control chrysopa septempunctata.
Description
Technical field
The present invention relates to a kind of method using rice moth egg breeding chrysopa septempunctata larva, belong to agriculture raising field.
Background technology
Chrysopa septempunctata (Chrysopa septempunctata Wesmael) belongs to Neuroptera, chrysopidae, Chrysopa subfamily, big grass
Sandfly category, is a kind of very wide predatory natural enemy insect of distribution.Due to its feeding habits are wide, heavy, distribution is wide, more than quantity it is deep by
The attention of domestic and international biological and ecological methods to prevent plant disease, pests, and erosion worker.Using the Chrysopa pest control of nature, often by generation period, quantity, species and worm
State limits, and cannot gain the initiative in preventing and controlling.Pass through mass rearing, breeding and release, so that it may in a planned way to utilize grass
Sandfly carrys out pest control.
China's Chrysopa type and quantity are very abundant, recorded totally 109 kinds of 18 categories, the overwhelming majority belongs to Chrysopa subfamily, agriculture
Woods is common kind more than 10.In addition to chrysopa septempunctata has no report except Tibet, the Inner Mongol, other areas have.Chrysopa septempunctata category holometabolan,
Larval phase, casts off a skin twice, there is three ages, and larva is double sucking mouth parts, and adult is biting mouthparts.Its adult and larva are equal
Prey on aphid.Multiple generations occurred in 1 year for chrysopa septempunctata, there is obvious generation overlap phenomenon.It occurs from generation to generation often with spot
Latitude south move and increase.Adult has phototaxis, upward property, and ovum gathers production, produces on plants shoots, blade, bark etc., sometimes
Also produce on wall, on window, or even production, on fluorescent lamp, the base portion of ovum has a silk handle.According to biology department of Hubei University insect day
Research department's research is opposed, the female eggs on average amount of chrysopa septempunctata list is 800 or so, most up to 1234.Adult has feeding ovum grain
Habit, therefore to take ovum immediately in artificial feeding.Its larva is also known as aphis lion because food aphid amount is big.The feeding of 1,2 ages is smaller, and 3 ages took
Food is very big, accounts for 80% or so of larval phase total appetite.In Nanjing breeding observing, chrysopa septempunctata larva all one's life edible aphid 677.6,
Adult is edible 488.22 average, and a generation, each Chrysopa average energy eliminated 1041.44 aphids.Larva has what is killed each other
Habit, is become apparent with 3 instar larvaes especially, and larva, which reels off raw silk from cocoons to cocoon, to pupate.Mainly with mature larva deadwood, fallen leaves heap, tree seam or
Cocoon in wizened and wrinkly crimping blade overwintering.Its preoviposition period is longer, and generally 4-18 days, a mating can produce embryonated egg throughout one's life, with
The oviposition of evening 8-10 points is most.
Although Chrysopa feeding habits are relatively wide, as the feed needed for mass rearing breeding Chrysopa, it is necessary to possess two conditions.Its
One, Chrysopa likes feeding, and has no adverse effects to Chrysopa ontogeny and reproductive capacity；Second, deriving from a wealth of sources, it is readily available,
It is of low cost, easy to store.With natural food raising Chrysopa effect it is good, but often because of the influence of natural conditions (such as weather) and by
To limitation, in order to greatly develop biological control science and technology, realize the large-scale production of Dominant Natural Enemies, Dominant Natural Enemies people should be strengthened
The research of work feed.Then the research of man-made feeds replacement natural feed is carried out immediately.Since the sixties, in order to solve to advise greatly
The problem of mould breeding Chrysopa in production for utilizing, many entomology workers have carried out the research of man-made feeds both at home and abroad, but imitate
Fruit is not notable.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of method using rice moth egg breeding chrysopa septempunctata larva, this hair
Bright processing from rice moth egg, the collection of chrysopa septempunctata ovum and storage, the free-ranging of chrysopa septempunctata larva, pupate receive cocoon, development is sprouted wings and different
The key technique of each raisings such as the refrigeration of worm state product, has carried out research and probe, to utilize rice moth egg biological control
Chrysopa septempunctata provides scientific basis.
The technical solution that the present invention solves above-mentioned technical problem is as follows：A kind of side using rice moth egg breeding chrysopa septempunctata larva
Method, including：
1) rice moth egg is obtained
Under conditions of 22-28 DEG C of temperature, relative humidity 70%, rice moth egg is inoculated into rice moth larva artificial feed,
Rice moth egg hatching be rice moth larva after, feeding man-made feeds growth and development, rice moth larva raising turned into after 40-45 days rice moth into
Worm, rice moth imago is moved into ovigerous disk, is allowed to lay eggs, 24 it is small when after collect rice moth egg, remove scale and feed impurity, and pass through
Ultraviolet kills embryo processing, then rice moth egg is sticked on paper, obtains rice moth egg card, spare；
2) chrysopa septempunctata is raised
The rearing conditions of chrysopa septempunctata each age are：25-27 DEG C of temperature, relative humidity more than 75%, when illumination 14 is small；
2.1) adult is raised
Chrysopa septempunctata adult is put in raising cylinder, and every chrysopa septempunctata adult feeds what is 1) obtained by 150 rice moth egg/days of appetite
Rice moth egg card, each cylinder of raising have 20 female adults and 4-7 male worms, and mating removes male worm after 3 days, raising cylinder both ends are with individual layer
Cotton seals, and with sponge ball moisturizing, then after raising 8 days, is put into oviposition paper into raising cylinder, is adjacent to raising cylinder periphery of inner wall,
Morning next day collects oviposition paper and ovum grain；
2.2) the ovum phase raises
2.1) ovum card is made in the chrysopa septempunctata ovum collected, is directly used in inoculation reproduction, or ovum is made and is stuck in 10-12 DEG C of refrigeration
It is spare within 10 days；
2.3) larva free-ranging
It will be accessed with a batch of chrysopa septempunctata ovum in raising box, and per 30 chrysopa septempunctata ovum of box, raise box upper end with individual layer cotton
Cloth seals, and with wet sponge ball moisturizing, after chrysopa septempunctata larva sprouts wings, puts spacer in raising box, prevents it from killing each other, and
Rice moth egg card is fed, specific injected volume is：Every 1-2 instar larvae feeds 50 rice moth egg/days；Larva feeding 200 after every 3 ages
Grain rice moth egg/day, replaces a rice moth egg card in every 3 days, until it is pupated；
2.4) pupa time receives cocoon
Access chrysopa septempunctata ovum the 8th day, paper of pupating is placed in box is raised, traps and pupates, then pupa is collected into cultivation and is held
In device, it is placed at 8 DEG C within preserving 50 days, it is spare.
2.5) emergence of adult
Pupa is put into raising box and is developed, in addition to it need not supply its food, other conditions are the same as 2.3) larva free-ranging, pupa development 9-
Chrysopa septempunctata adult is turned into after 12 days, chrysopa septempunctata adult directly raises oviposition or within 10 DEG C refrigerate 80 days, spare；
2.6) refrigeration of product
Chrysopa septempunctata pupa is within 8 DEG C preserve 50 days；Chrysopa septempunctata adult is within 8-10 DEG C preserves 100 days；Chrysopa septempunctata ovum exists
Within 8-10 DEG C refrigerates 10 days, the relative humidity during refrigeration is 50%-80%；
2.7) pack
Chrysopa septempunctata adult is box-packaged, and chrysopa septempunctata larva and chrysopa septempunctata ovum are packed with paper bag type.
Based on the above technical solutions, the present invention can also be improved as follows.
Further, in 2.1), a diameter of 15 centimetres, a height of 25 centimetres of the raising cylinder.
Further, in 2.3), the specification of the raising box：15 centimetres of diameter is 10 centimetres high.
Further, in 2.3), the spacer put in the raising box is corrugated paper or paper slip.
The beneficial effects of the invention are as follows：
Chrysopa septempunctata artificial propagation at present uses live body host insect more, and for feed, complex procedures, occupied space is big, manually into
This height, and pollution and the harm of other Chrysopa natural enemies are be easy to cause, production process is difficult to control, and complicated, raising quality is not
Stablize, while because being mixed with host insect, be easy to cause pollution, and can not each worm state of chrysopa septempunctata raising be controlled with
The collection of product, it is difficult to reach batch production and largely produce, it is impossible to meet the needs of green agriculture and crop production safety.Urgently
Need the preferable Hosts of screening raising chrysopa septempunctata effect and its method for breeding.Feeding rice moth egg is that current raising chrysopa septempunctata is comprehensive
Close and evaluate best Hosts, but there has been no the reproduction technique system using rice moth egg feeding chrysopa septempunctata at present.The present invention's
Propagation method can quantify and control each production link, ensure product quality, easy to be factory produced.
Brief description of the drawings
Fig. 1 is influence figure of the temperature (DEG C) to chrysopa septempunctata development duration (hour)；
Fig. 2 is the influence figure of temperature (DEG C) and relative humidity (RH) to chrysopa septempunctata development duration (hour).
Embodiment
The principles and features of the present invention are described below, and the given examples are served only to explain the present invention, is not intended to limit
Determine the scope of the present invention.
Influence of 1 Three feed of embodiment to chrysopa septempunctata growth and development
1 materials and methods
Foodstuff is made using Trichogramma pupae, rice moth egg and aphid, in glass raising cylinder (500ml) with paper slip make every
Just chrysopa septempunctata larva is incubated from thing group breeding.Addition adequately feed daily, cocoon of pupating in time daily to after pupating.Every kind of processing is raised
Support 5.Observe and record that larva goes through the phase, pupa goes through the phase, percentage of pupation, eclosion rate daily.After cocooing 3 days, claim pupa weight with electronic scale.Observation
And record each worm state survival condition.
Temperature is 27 ± 1 DEG C during experiment, and photoperiod L: D=14: 10, relative humidity is 70 ± 5%.
2 results and analysis
Influence of the 2.1 different feeds processing to chrysopa septempunctata growth and development
The development duration difference between Three feed processing as it can be seen that chrysopa septempunctata larva is averaged of table 1, from be short to it is long successively
For：Aphid (8.08 days), rice moth egg (8.20 days), Trichogramma pupae (8.61 days).
The different foods of table 1 go through chrysopa septempunctata larvae development the influence of phase
* (development duration：M±SD)
But chrysopa septempunctata be averaged pupa go through phase and eclosion rate Three feed processing between there is no difference；But it is poor that percentage of pupation exists
Different, Trichogramma pupae processing the average change into pupa rate is 58.8%, is raised less than with rice moth egg (63.20%) and aphid
(69.20%)；The pupa of two feed processing is significantly higher than aphid processing again, is shown in Table 2.
Influence of the different foods of table 2 to chrysopa septempunctata pupa time and emergence
* (being the arcsin value of percentage in bracket)
Table 3 is as can be seen that the first-instar young death rate of each processing is zero；But the 2nd, the death rate of third-instar larvae is throughout
Had differences between reason；In terms of whole children phase survival rate, aphid processing highest, rice moth egg processing is taken second place, at Trichogramma pupae
Manage it is minimum, but more than 90%.Experimental observation finds in Trichogramma pupae processing, there is quite a few adult eclosion
Dead quickly afterwards, adult pick-up rate (57.78%) is substantially less than rice moth egg (77.78%) and the knot of aphid (86.67%) raising
Fruit.
3 different feeds of table handle chrysopa septempunctata larval mortality % and adult pick-up rate %
* (being the arcsin value of percentage in bracket)
The hatching of 2 chrysopa septempunctata ovum of embodiment and refrigerated condition
1 materials and methods
The influence of 1.1 epidemic disasters
Treatment temperature gradient is set to 15 DEG C, 20 DEG C, 25 DEG C, 30 DEG C, and humidity sets 35% and 70%.When collection chrysopa septempunctata 12 is small
Interior produced fresh ovum, often dispenses 20, each temperature gradient 5 is managed in dactylethrae.It is repeated 3 times.Observation egg development situation daily, when
When ovum grain surface stain is hatched soon, it is every 2 it is small when observation once.Record the development duration and hatching situation of each processing ovum.
The refrigeration of 1.2 chrysopa septempunctata ovum
The ovum for being developed to different times at 27 DEG C is taken respectively：Within when postpartum 8 is small, development 24-32 it is small when and development 48-
56 it is small when, be respectively charged into finger-type pipe, often pipe 80.According to the threshold of development of ovum, the ovum of each developmental stage is distinguished
If 4 DEG C, 7 DEG C, 10 DEG C and 13 DEG C this 4 temperature gradients are refrigerated.Each treatment temperature was every 3 days, 6 days, 9 days, 12 natural gift
20 ± 5 are taken out not from each processing, dactylethrae is sub-packed in, is placed in 27 DEG C, under conditions of relative humidity is 70%, record
Development duration and incubation rate.In triplicate.
2 results and analysis
The development of 2.1 chrysopa septempunctata ovum and the relation of temperature
The development duration and temperature relation of 4 ovum of table
* (development duration：Mean+SD)
The rate of development that can be seen that ovum from table 4 and Fig. 1 is proportionate with the rise of temperature.According to formula T=KV+C, T
For environment temperature；K is effective accumulated temperature；V represents rate of development；C is threshold of development.Egg development origin temp can be calculated
And effective accumulated temperature：
Return law of the straight line：
K=1/V × (T-C)
Wherein K is effective accumulated temperature, and V is developmental rate, and T is environment temperature, and C is threshold of development.Calculate chrysopa septempunctata
The threshold of development of ovum is C=9.15 DEG C, and K=57.13 subsists.
Direct optimization (Li Dianmo etc., 1986)：
Threshold of development：Effective accumulated temperature：
Wherein Ai=Di(Ti- C), AiFor the effective accumulated temperature in threshold of development C, TiTo test set temperature,
DiFor in TiAt a temperature of development duration.C=9.69 DEG C, K=54.54 subsists.The development of 2.2 chrysopa septempunctata ovum and the relation of humidity
It can be seen that from table 5 and Fig. 2 under conditions of humidity 35%, the incubation rate of ovum is with the reduction and development of temperature
Time lengthening and decline；At 15 DEG C, the incubation rate of ovum is 48%.But under conditions of humidity 70%, incubation rate and the temperature of ovum
Do not have significant correlation between degree change.
Influence of the different humitures of table 5 to egg hatching rate (%)
* (being the arcsin value of percentage in bracket)
The refrigeration of 2.3 ovum
6 are the results are shown in Table, ovum cannot hatch at 4 DEG C after refrigeration in 3 days；Under the conditions of 7 DEG C, ovum incubates after refrigerating 3 days
Rate is only 30%.Optimal refrigerated storage temperature is 13 DEG C, after refrigeration 9 days its hatching can also reach more than 80%, but with refrigeration
The extension of time, the incubation rate of ovum gradually reduce.Do not had differences between the ovum processing of different developmental phases.
The incubation rate of 6 ovum of table is acted on refrigeration
* (being the arcsin value of percentage in bracket)
The influence of 3 stocking density of embodiment and temperature to killing each other
1 materials and methods
1.1 stocking density
In the circle box of 15 × 10 (diameter * high) raise 1 instar larvae of chrysopa septempunctata, density be respectively per box 30,60,90,
120th, 150, isolated with wide 5mm paper slips, 3 repetitions, are fed for enough aphids.Physics and chemistry pupa (into cocoon) rate everywhere is recorded in observation.
And independently raised with single head to compare.Temperature is 27 DEG C during experiment, photoperiod L: D=14: 10, relative humidity for 70 ±
5%.
1.2 raising temperature
At 20 DEG C, 25 DEG C, 30 DEG C, larvae at the beginning of every box group breeding 50.3 repetitions.50 childrens are raised in every
Physics and chemistry pupa (into cocoon) rate everywhere is recorded in worm, observation.And independently raised with single head to compare.The photoperiod is L: D=14 during experiment:
10, relative humidity is 70 ± 5%.
1.3 different feeds
In addition to feed processing is respectively aphid, rice moth egg, Trichogramma pupae, remaining is the same as 1.1.
2 results and analysis
Influence of 2.1 stocking densities to chrysopa septempunctata
As can be seen from Table 7, as the increase of density, percentage of pupation are more and more lower.In the case of low density, kill each other
It can not avoid.Such as when density is 30, percentage of pupation 75.34%.When density increases to 120 every, the pick-up rate of pupa is only
50.52%.The stocking density of every 30, box may be selected in biological control.
The percentage of pupation (%) raised under 7 larva different densities of table
* (being the arcsin value of percentage in bracket)
Influence of 2.2 raising temperatures to chrysopa septempunctata
As can be seen from Table 8, under each temperature conditionss, the eclosion rate of cocoon and larva are gone through in group breeding and individually raising
Phase does not make significant difference simultaneously, and is had a significant impact for percentage of pupation.With the reduction of temperature, group breeding, which is pupated, to increase, and puts down
Equal percentage of pupation is 68% at 30 DEG C, and 25 DEG C of whens are 70.70%, and when temperature is 20 DEG C, percentage of pupation brings up to 74.8%.
8 different temperatures of table is to effect of killing each other
* (being the arcsin value of percentage in bracket)
Influence of 2.3 feeds to chrysopa septempunctata
Influence of 9 different feeds of table to killing each other
As can be seen from Table 9, different feeds processing has influenced percentage of pupation, but individually raising, and percentage of pupation is from high to low
Respectively aphid (97.78%), rice moth egg (95.56%), Trichogramma pupae (91.11%), but difference is not notable.And
Group breeding is still the percentage of pupation highest of bean aphid raising, is 69.20%, and the percentage of pupation of Trichogramma pupae raising is most
It is low, it is only 58.80%, the percentage of pupation of rice moth egg raising is 63.20%, the significant difference between three.
The foregoing is merely presently preferred embodiments of the present invention, is not intended to limit the invention, it is all the present invention spirit and
Within principle, any modification, equivalent replacement, improvement and so on, should all be included in the protection scope of the present invention.
Claims (4)
- A kind of 1. method using rice moth egg breeding chrysopa septempunctata larva, it is characterised in that including：1) rice moth egg is obtainedUnder conditions of 22-28 DEG C of temperature, relative humidity 70%, rice moth egg is inoculated into rice moth larva artificial feed, rice moth Egg hatching be rice moth larva after, feeding man-made feeds growth and development, rice moth larva raising turns into rice moth imago after 40-45 days, Rice moth imago is moved into ovigerous disk, is allowed to lay eggs, 24 it is small when after collect rice moth egg, remove scale and feed impurity, and through purple Embryo processing is killed in outside line, then rice moth egg is sticked on paper, obtains rice moth egg card, spare；2) chrysopa septempunctata is raisedThe rearing conditions of chrysopa septempunctata each age are：25-27 DEG C of temperature, relative humidity more than 75%, when illumination 14 is small,2.1) adult is raisedChrysopa septempunctata adult is put in raising cylinder, and every chrysopa septempunctata adult is fed the rice moth 1) obtained by 150 rice moth egg/days of appetite Ovum card, each cylinder of raising have 20 female adults and 4-7 male worms, and mating removes male worm after 3 days, raising cylinder both ends are with individual layer cotton Sealing, with sponge ball moisturizing, then after raising 8 days, is put into oviposition paper into raising cylinder, raising cylinder periphery of inner wall is adjacent to, in next day Collect oviposition paper and ovum grain in the morning；2.2) the ovum phase raisesBy 2.1) collect chrysopa septempunctata ovum ovum card is made, be directly used in inoculation reproduction, or be made ovum be stuck in 10-12 DEG C refrigerate 10 days Within, it is spare；2.3) larva free-rangingIt will be accessed with a batch of chrysopa septempunctata ovum in raising box, per 30 chrysopa septempunctata ovum of box, raising box upper end is sealed with individual layer cotton Mouthful, with wet sponge ball moisturizing, after chrysopa septempunctata larva sprouts wings, spacer is put in raising box, prevents it from killing each other, and feed Rice moth egg card, specific injected volume are：Every 1-2 instar larvae feeds 50 rice moth egg/days；Larva feeds 200 rice after every 3 ages Moth ovum/day, replaces a rice moth egg card in every 3 days, until it is pupated；2.4) pupa time receives cocoonAccess chrysopa septempunctata ovum the 8th day, paper of pupating is placed in box is raised, traps and pupates, then pupa is collected into and is cultivated in container, put It is spare within being preserved 50 days at 8 DEG C；2.5) emergence of adultPupa is put into raising box and is developed, in addition to it need not supply its food, other conditions are the same as 2.3) larva free-ranging, pupa development 9-12 days After turn into chrysopa septempunctata adult, chrysopa septempunctata adult directly raises oviposition or within 10 DEG C of refrigeration 80 days, spare；2.6) refrigeration of productChrysopa septempunctata pupa is within 8 DEG C preserve 50 days；Chrysopa septempunctata adult is within 8-10 DEG C preserves 100 days；Chrysopa septempunctata ovum is in 8-10 DEG C refrigeration 10 days within, the relative humidity during refrigeration is 50%-80%；2.7) packChrysopa septempunctata adult is box-packaged, and chrysopa septempunctata larva and chrysopa septempunctata ovum are packed with paper bag type.
- 2. according to the method described in claim 1, it is characterized in that, in 2.1), raise cylinder a diameter of 15 centimetres are high For 25 centimetres.
- 3. according to the method described in claim 1, it is characterized in that, in 2.3), the specification of the raising box：15 lis of diameter Rice is 10 centimetres high.
- 4. according to the method described in claim 1, it is characterized in that, in 2.3), the spacer put in the raising box is watt Stupefied paper or paper slip.
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| CN107466975B (en) * | 2017-09-25 | 2023-06-27 | 广西壮族自治区农业科学院甘蔗研究所 | Multifunctional rice moth feeding device |
| CN107683828B (en) * | 2017-10-05 | 2021-05-18 | 福建省农业科学院植物保护研究所 | Self-disabled insect feeding and egg collecting method |
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