CN107182943A - A kind of method of indoor mass rearing Bradysia fungus gnat - Google Patents
A kind of method of indoor mass rearing Bradysia fungus gnat Download PDFInfo
- Publication number
- CN107182943A CN107182943A CN201710350446.0A CN201710350446A CN107182943A CN 107182943 A CN107182943 A CN 107182943A CN 201710350446 A CN201710350446 A CN 201710350446A CN 107182943 A CN107182943 A CN 107182943A
- Authority
- CN
- China
- Prior art keywords
- fungus gnat
- culture
- bradysia fungus
- culture dish
- bradysia
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241001157791 Mycetophilus sp. HMR-1993 Species 0.000 title claims abstract description 76
- 241001494113 Bradysia Species 0.000 title claims abstract description 72
- 238000000034 method Methods 0.000 title claims abstract description 23
- 230000000384 rearing effect Effects 0.000 title claims abstract description 18
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims abstract description 24
- 235000007685 Pleurotus columbinus Nutrition 0.000 claims abstract description 17
- 240000001462 Pleurotus ostreatus Species 0.000 claims abstract description 17
- 235000001603 Pleurotus ostreatus Nutrition 0.000 claims abstract description 17
- 238000004519 manufacturing process Methods 0.000 claims abstract description 7
- 239000011159 matrix material Substances 0.000 claims abstract description 7
- 238000011081 inoculation Methods 0.000 claims abstract description 5
- 239000001963 growth medium Substances 0.000 claims description 27
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 18
- 241000382353 Pupa Species 0.000 claims description 12
- 238000012258 culturing Methods 0.000 claims description 10
- 235000014655 lactic acid Nutrition 0.000 claims description 9
- 239000004310 lactic acid Substances 0.000 claims description 9
- 239000002609 medium Substances 0.000 claims description 9
- 244000144987 brood Species 0.000 claims description 8
- 235000013305 food Nutrition 0.000 claims description 7
- 230000012447 hatching Effects 0.000 claims description 7
- 235000013601 eggs Nutrition 0.000 claims description 6
- 238000012360 testing method Methods 0.000 claims description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 5
- 239000000356 contaminant Substances 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 claims description 4
- 241000607479 Yersinia pestis Species 0.000 claims description 3
- 210000001015 abdomen Anatomy 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 230000032669 eclosion Effects 0.000 claims description 3
- 239000012528 membrane Substances 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 238000011534 incubation Methods 0.000 claims 1
- 238000009395 breeding Methods 0.000 abstract description 4
- 230000001488 breeding effect Effects 0.000 abstract description 4
- 241000238631 Hexapoda Species 0.000 abstract description 3
- 230000001932 seasonal effect Effects 0.000 abstract description 3
- 241000196324 Embryophyta Species 0.000 description 6
- 239000001965 potato dextrose agar Substances 0.000 description 6
- 102000002322 Egg Proteins Human genes 0.000 description 5
- 108010000912 Egg Proteins Proteins 0.000 description 5
- 238000002474 experimental method Methods 0.000 description 5
- 210000004681 ovum Anatomy 0.000 description 5
- 241000233866 Fungi Species 0.000 description 3
- 241000256095 Sciaridae Species 0.000 description 3
- 230000001418 larval effect Effects 0.000 description 3
- 239000000575 pesticide Substances 0.000 description 3
- 240000006108 Allium ampeloprasum Species 0.000 description 2
- 241000255925 Diptera Species 0.000 description 2
- 241000255932 Nematocera Species 0.000 description 2
- 244000061456 Solanum tuberosum Species 0.000 description 2
- 235000002595 Solanum tuberosum Nutrition 0.000 description 2
- 238000004500 asepsis Methods 0.000 description 2
- 238000009835 boiling Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000012549 training Methods 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- 241000222532 Agrocybe Species 0.000 description 1
- 235000005254 Allium ampeloprasum Nutrition 0.000 description 1
- 235000017060 Arachis glabrata Nutrition 0.000 description 1
- 244000105624 Arachis hypogaea Species 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 235000018262 Arachis monticola Nutrition 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241001157808 Mycetophilidae Species 0.000 description 1
- 241000244206 Nematoda Species 0.000 description 1
- 241000134360 Sciaroidea Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000002431 foraging effect Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 230000029264 phototaxis Effects 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 238000005057 refrigeration Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/90—Feeding-stuffs specially adapted for particular animals for insects, e.g. bees or silkworms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Polymers & Plastics (AREA)
- Environmental Sciences (AREA)
- Animal Husbandry (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Birds (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Animal Behavior & Ethology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Insects & Arthropods (AREA)
- Mushroom Cultivation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a kind of method of indoor mass rearing Bradysia fungus gnat, belong to plant protection and cultured insect technical field.Methods described raises Bradysia fungus gnat using the hypha of Pleurotus ostreatus of culture dish culture, realize successive propagation, the step of including culture matrix manufacturing, hypha of Pleurotus ostreatus inoculated and cultured, the acquisition of Bradysia fungus gnat kind worm and continuously rearing, the peaceful mushroom mycelium inoculation of culture matrix manufacturing is completed on superclean bench.The present invention raises Bradysia fungus gnat using mycelia, it is to avoid the shortcoming being subject to seasonal restrictions is raised by fresh host plant.The method for breeding of the present invention saves artificial, simple to operate.Only need to operation 23 times a growth cycle for Bradysia fungus gnat, be greatly saved man-hour.
Description
Technical field
The present invention relates to a kind of method of indoor mass rearing Bradysia fungus gnat, belong to plant protection and cultured insect technology neck
Domain.
Background technology
Eye Sciaridae (Sciaridae) belongs to Diptera (Diptera) Nematocera (Nematocera) eye fungus gnat Superfamily
(Sciaroidea), species is enriched, widely distributed, likes moist dark environment, adaptable.The larva of most of population
In the excrement for living in withered trees, fungi and animal, some larvas are also lived in cave, vegetable plot, greenhouse.Eye fungus gnat life
Cycle living is usually 3~4 weeks, Life of Adult 3~5 days, 4~7 days ovum phases, 9~l of larval phase 7 days, 3~5 days pupa time；Female adult one
As can lay eggs 70~200, being originated in ovum more soil seam between gap, plant base portion and leaf sheath gap in.
Bradysia fungus gnat (Bradysia spp.) is that one of more, harm most serious category, the whole world are studied in a Sciaridae
Known to have 455 kinds, what China had recorded has 131 kinds；Widely distributed, foraging territories are wide, the various vegetables that can cause harm and edible mushroom,
Crop can be caused to have no harvest when serious.The many clusters in plant roots and stems and root surrounding soil gap of larva are survived the winter, the Winter in North China phase
For mid or late October~mid or late November, start to pupate in next year mid or late March, April, early and middle ten dayses reached emergence peak；Get in south
Teletostage is mid or late December, starts to pupate in late Febuary in next year, reaches emergence peak mid-March.4~June, late September~11
Month worm amount is more, is hazard boom in season in spring and autumn 2, and 7~August causes worm amount to die-off because of high temperature and drought.
Bradysia fungus gnat larva mainly takes food the under ground portion for plant of causing harm and the mycelium of edible mushroom, fructification etc., may be used also
Propagate disease；Adult has stronger phototaxis, though pathogen, nematode and mite class etc. can be propagated by not taking food, is connected between causing
Evil.Bradysia fungus gnat individual is small, reproduction speed fast, it is hidden to cause harm, and preventing and treating is difficult and host range is wide, has become in agricultural production
The problem of urgent need to resolve.Especially in Edible Fungi, Bradysia fungus gnat can cause harm a variety of foods such as flat mushroom, agrocybe, White mushroom
With bacterium, the yield and quality of edible mushroom is had a strong impact on.And the current domestic registered insecticide that can be used on edible mushroom
It is less；The edible fungi growth cycle is short, and it is exceeded that long-term irrational use agricultural chemicals easily causes product residues of pesticides, produces insect anti-
The property of medicine.Therefore need to carry out testing sieve select to the edible mushroom preferable biological pesticide of Bradysia fungus gnat effect and efficiently, it is low toxicity, low residual
The chemical pesticide stayed carrys out Instructing manufacture medication.And, it is necessary to which a large amount of worm ages are consistent, develop in Chemicals are studied with integrated control
Neat larva is used as test worm.The artificial feeding of current Bradysia fungus gnat is more using fresh host plant or in vitro rhizome as feed,
There is season limit and cost is higher, also use the food such as man-made feeds or peanut, potato, soybean to carry out Scale dependency for feed
Bradysia fungus gnat, but operation is more complicated, it is time-consuming to take a lot of work.
The content of the invention
For the method for breeding of existing Bradysia fungus gnat, in order to which the experiment for better meeting the research of edible mushroom Bradysia fungus gnat is needed
Ask, realize the extensive raising of Bradysia fungus gnat, inventor is used as feed there is provided one kind by long-term experiment by the use of hypha of Pleurotus ostreatus
The method for raising Bradysia fungus gnat.The present invention can realize the anniversary mass rearing of Bradysia fungus gnat, and simple to operate, save work
When, it is cost-effective, be adapted to obtain in laboratory and largely develop consistent Bradysia fungus gnat larva and supply experimental study.
A kind of method for indoor mass rearing Bradysia fungus gnat that the present invention is provided, is raised using the hypha of Pleurotus ostreatus of culture dish culture
Bradysia fungus gnat is supported, successive propagation, including culture matrix manufacturing-hypha of Pleurotus ostreatus culture-Bradysia fungus gnat kind worm acquisition-Bradysia fungus gnat is realized
The step of continuously rearing, wherein the peaceful mushroom cultural hypha of culture matrix manufacturing is completed on superclean bench.Comprise the following steps that：
Step one：PDA culture medium makes.
(1) weigh finished product PDA culture medium 39g, 1000mL water to pour into beaker, bevelling stirring in side obtains culture medium solution；
It is preferred that, described finished product PDA culture medium and water can be heated in the water-bath of boiling, promote dissolving.
(2) culture medium solution dissolved is dispensed into 4 500mL triangular flasks, mouth is sealed with sealed membrane and rubber band,
121 DEG C sterilize 20 minutes.
(3) when temperature drops to 50~60 DEG C after the completion of sterilizing, lactic acid is added in culture medium solution.Per 1000mL culture mediums
The μ L of lactic acid 200 are added in solution, are shaken up.The mass percent concentration of the lactic acid is 25%.
(4) culture medium solution for having added lactic acid is poured into diameter 9cm culture dish, culture medium solution in culture dish is blown
After dry, dehydrated medium is obtained standby.
Step 2：Hypha of Pleurotus ostreatus inoculation, culture.
(1) actication of culture.The hypha of Pleurotus ostreatus in test tube is scraped with oese, the culture dish with dehydrated medium is inoculated into
On.The culture dish being inoculated with is placed in 25 DEG C of incubators, 24 hours dark culturings, 5 days or so mycelia are covered with and can be enlarged
Culture.
(2) squamous subculture.The dehydrated medium that mycelia is covered with culture dish is divided into some fritters, new tool is inoculated into
Have on the culture dish of dehydrated medium, 24 hours dark culturings are carried out in 25 DEG C of incubators.The culture dish after mycelia is covered with to put
Enter (4~6 DEG C of temperature) stored refrigerated in refrigerator, raised for Bradysia fungus gnat.
Note：Note stringent asepsis requirements in the peaceful mushroom silk seeded process of culture matrix manufacturing, it is to avoid living contaminants.
Step 3：Bradysia fungus gnat kind worm obtains.
The Bradysia fungus gnat four-age larva or pupa in the mushroom rod caused harm by Bradysia fungus gnat, picking mushroom rod are collected in mushroom shed, is put
Enter in the culture dish for covering with mycelia in step 2 after squamous subculture；The culture dish for accessing Bradysia fungus gnat is put into 25 DEG C of incubators
After middle dark culturing, four-age larva culture 5~7 days, after pupa culture 3~4 days, adult eclosion.The adult allowed after sprouting wings is being covered with
Pangamy is laid eggs in the culture dish of mycelia, and the first brood of larvae of hatching is used as kind of a worm.
Also the more bulging female adult of belly and male worm are collected in the serious mushroom shed that can be caused harm with pest sucking device in Bradysia fungus gnat, step is put into
Raised in the culture dish for covering with mycelia in rapid two after squamous subculture, it is dark 1~2 day in 25 DEG C of incubators after Adult worms producting eggs,
The first brood of larvae of hatching is used as kind of a worm.
Step 4：Bradysia fungus gnat continuously rearing.
(1) culture dish for covering with mycelia after the squamous subculture prepared with step 2 raises the Bradysia fungus gnat the in step 3
Generation larva is to pupate within 8~12 days after kind of a worm, larvae hatch.
(2) first brood of larvae of Bradysia fungus gnat is after kind of worm has taken food the hypha of Pleurotus ostreatus in the culture dish for covering with mycelia,
The culture medium with larva is divided into some pieces with scalpel or pincet, is respectively put into the new culture dish for covering with mycelia,
Continue to raise.
(3) after Bradysia fungus gnat pupates, pupa is chosen in the new culture dish for covering with mycelia with line pen is hooked, carries out subculture numerous
Grow.
Note：Under the conditions of 25 DEG C, pupa time and ovum phase are distinguished 3~4 days, 8~12 days larval phases, can be chosen not according to experiment demand
Bradysia fungus gnat larva of the same period is tested.
The invention has the advantages that：
(1) present invention raises Bradysia fungus gnat using mycelia, it is to avoid raise what is be subject to seasonal restrictions by fresh host plant
Shortcoming.
(2) method for breeding of the invention saves artificial, simple to operate.Only need to operation a growth cycle for Bradysia fungus gnat
2~3 times, it is greatly saved man-hour.
Brief description of the drawings
The method flow diagram for the indoor mass rearing Bradysia fungus gnat that Fig. 1 provides for the present invention.
Embodiment
The present invention is described in detail with reference to the accompanying drawings and examples.
The present invention provides a kind of method of indoor mass rearing Bradysia fungus gnat, and flow as shown in Figure 1 specifically includes following step
Suddenly：
Step one：PDA culture medium makes.
(1) electronic scale weighs finished product PDA culture medium (potato dextrose agar) 39g, pours into 2000mL beakers.Take
1000mL water is poured into beaker, the stirring of side bevelling.It can be placed in boiling water bath and heat, promote dissolving.
(2) culture medium dissolved is dispensed into 4 500mL triangular flasks, mouth, 121 DEG C is sealed with sealed membrane and rubber band
Sterilizing 20 minutes.
(3) when temperature drops to 50~60 DEG C after the completion of sterilizing, culture medium is taken out, is operated in superclean bench, breast is added
Acid.The μ L of lactic acid 200 are added per 1000mL culture mediums, are shaken up.The mass percent concentration of the lactic acid is 25%.
(4) in superclean bench, in the culture dish that the culture medium for having added lactic acid is poured into diameter 9cm, culture medium drying
It is standby that the culture dish with dehydrated medium is obtained afterwards.
Step 2：Hypha of Pleurotus ostreatus inoculation, culture.
(1) actication of culture.The hypha of Pleurotus ostreatus in test tube is scraped with oese in superclean bench, step one is inoculated into
On culture dish with dehydrated medium, it is ensured that gnotobasis, it is to avoid living contaminants.The culture dish being inoculated with is placed on 25 DEG C of trainings
Support in case, 24 hours dark culturings, 5 days or so mycelia, which are covered with, can be enlarged culture.
(2) squamous subculture.The culture medium for covering with mycelia is divided into some fritters in superclean bench, is inoculated into new
On dehydrated medium, 24 hours dark culturings are carried out in 25 DEG C of incubators.The culture dish for covering with mycelia is put into refrigeration in refrigerator
Preserve, raised for Bradysia fungus gnat.
Note：Note stringent asepsis requirements in the peaceful mushroom silk seeded process of culture matrix manufacturing, it is to avoid living contaminants.
Step 3：Bradysia fungus gnat kind worm obtains.
The Bradysia fungus gnat kind worm obtains to be realized by any one in the following two kinds mode：
(1) mushroom rod caused harm by Bradysia fungus gnat is collected in mushroom shed, laboratory is taken back.Bradysia fungus gnat four in picking mushroom rod
Instar larvae or pupa, are put into the culture dish for covering with mycelia；The culture dish for covering with mycelia for accessing Bradysia fungus gnat is put into 25 DEG C of trainings
Support after dark culturing in case, four-age larva culture 5~7 days, after pupa culture 3~4 days, adult eclosion.The adult allowed after sprouting wings exists
Cover with pangamy in the culture dish of mycelia to lay eggs, the first brood of larvae of hatching is used as kind of a worm.
(2) the more bulging female adult of belly and male worm are collected in the serious mushroom shed that can also be caused harm with pest sucking device in Bradysia fungus gnat, is put
Enter in the culture dish for covering with mycelia in step 2 after squamous subculture and raise, in 25 DEG C of incubators dark culturing after 1~2 day into
Worm lays eggs, and the first brood of larvae of hatching is used as kind of a worm.
Step 4：Bradysia fungus gnat continuously rearing.
(1) Bradysia fungus gnat first brood of larvae, i.e., the kind worm obtained in step 3, larva are raised with the culture dish for covering with mycelia
Pupate within 8~12 days after hatching.
(2) after Bradysia fungus gnat larva has taken food the hypha of Pleurotus ostreatus in the culture dish for covering with mycelia, with scalpel or small tweezer
Culture medium with larva is divided into some pieces by son, is respectively put into the new culture dish for covering with mycelia, continues to raise.
(3) after Bradysia fungus gnat pupates, pupa is chosen in the new culture dish for covering with mycelia with line pen is hooked, carries out subculture numerous
Grow.
Note：Under the conditions of 25 DEG C, the pupa time of Bradysia fungus gnat and ovum phase are distinguished 3~4 days, 8~12 days larval phases, can be according to experiment
The Bradysia fungus gnat larva that demand chooses different times is tested.
Under the conditions of 25 DEG C, Bradysia fungus gnat is raised with potted plant leek seedling, completing a generation (from ovum to adult) needs 23~25
My god, it can obtain 150~200 larvas when accessing 5 pairs of Bradysia fungus gnat adults per basin leek seedling；It is that feed is raised late with fresh potato
Eye fungus gnat, completing a generation needs 20~23 days, and 200~300 larvas can be obtained when 5 pairs of Bradysia fungus gnat adults are accessed per box.This
The method that invention is provided raises Bradysia fungus gnat, and completing a generation needs 14~20 days, in each culture dish 5 pairs of Bradysia fungus gnats of access into
400~500 larvas can be obtained during worm.
The method feeding materials that the present invention is provided are hypha of Pleurotus ostreatus, are readily available, without seasonal limitation；Raising container is
Culture dish, takes up space small, can make mass propgation ware in incubator simultaneously and carry out anniversary breeding；Compared with other method, the present invention
The method of offer is easy to operate, saving working hour, and the cycle is short, and survival rate is high, can obtain and largely develop consistent larva for experiment.
Claims (5)
1. a kind of method of indoor mass rearing Bradysia fungus gnat, it is characterised in that：Comprise the following steps,
Step one：PDA culture medium makes；
(1) weigh finished product PDA culture medium 39g, 1000mL water to pour into beaker, bevelling stirring in side obtains culture medium solution；
(2) culture medium solution dissolved is attached in triangular flask, mouth is sealed with sealed membrane and rubber band, 121 DEG C sterilize 20 minutes；
(3) when temperature drops to 50~60 DEG C after the completion of sterilizing, lactic acid is added in culture medium solution；
(4) culture medium solution for having added lactic acid is poured into culture dish, after culture medium solution drying in culture dish, obtains drying
Culture medium is standby；
Step 2：Hypha of Pleurotus ostreatus inoculation, culture；
(1) actication of culture：The hypha of Pleurotus ostreatus in test tube is scraped with oese, is inoculated on the culture dish with dehydrated medium；
The culture dish being inoculated with is placed in 25 DEG C of incubators, 24 hours dark culturings；
(2) squamous subculture：The dehydrated medium that mycelia is covered with culture dish is divided into some fritters, new having is inoculated into and does
On the culture dish of dry culture medium, 24 hours dark culturings are carried out in 25 DEG C of incubators；
Step 3：Bradysia fungus gnat kind worm obtains；
Step 4：Bradysia fungus gnat continuously rearing；
(1) culture dish for covering with mycelia after the squamous subculture prepared with step 2 raises the Bradysia fungus gnat first generation in step 3
Larva is to pupate within 8~12 days after kind of a worm, larvae hatch；
(2) after Bradysia fungus gnat pupates, pupa is chosen in the new culture dish for covering with mycelia with line pen is hooked, carries out successive propagation.
2. a kind of method of indoor mass rearing Bradysia fungus gnat according to claim 1, it is characterised in that：Cultivate matrix manufacturing
With hypha of Pleurotus ostreatus inoculation, in incubation it is noted that aseptic condition, it is to avoid living contaminants.
3. a kind of method of indoor mass rearing Bradysia fungus gnat according to claim 1, it is characterised in that：By step one
It is stored refrigerated under the conditions of need to being placed on 4~6 DEG C with the culture dish for covering with hypha of Pleurotus ostreatus that step 2 is obtained, raised for Bradysia fungus gnat
With.
4. a kind of method of indoor mass rearing Bradysia fungus gnat according to claim 1, it is characterised in that：Institute in step 3
The Bradysia fungus gnat kind worm stated obtains, using the following two kinds mode：
The Bradysia fungus gnat four-age larva or pupa in the mushroom rod caused harm by Bradysia fungus gnat, picking mushroom rod are collected in mushroom shed, step is put into
In the culture dish for covering with mycelia in rapid two after squamous subculture；The culture dish for accessing Bradysia fungus gnat is put into black in 25 DEG C of incubators
After light culture, four-age larva culture 5~7 days, after pupa culture 3~4 days, adult eclosion；The adult allowed after sprouting wings is covering with mycelia
Culture dish in pangamy spawning, the first brood of larvae of hatching is used as kind of a worm；
Or the more bulging female adult of belly and male worm are collected in the serious mushroom shed that caused harm with pest sucking device in Bradysia fungus gnat, it is put into step 2
Raised in the culture dish for covering with mycelia after middle squamous subculture, dark culturing Adult worms producting eggs after 1~2 day in 25 DEG C of incubators,
The first brood of larvae of hatching is used as kind of a worm.
5. a kind of method of indoor mass rearing Bradysia fungus gnat according to claim 1, it is characterised in that：In step 4 late
After the larva of eye fungus gnat has taken food the hypha of Pleurotus ostreatus in the culture dish for covering with mycelia, larva will be carried with scalpel or pincet
Culture medium be divided into some pieces, be respectively put into the new culture dish for covering with mycelia, continue raise.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710350446.0A CN107182943B (en) | 2017-05-18 | 2017-05-18 | A kind of method of interior mass rearing Bradysia fungus gnat |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710350446.0A CN107182943B (en) | 2017-05-18 | 2017-05-18 | A kind of method of interior mass rearing Bradysia fungus gnat |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107182943A true CN107182943A (en) | 2017-09-22 |
| CN107182943B CN107182943B (en) | 2019-09-27 |
Family
ID=59873837
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710350446.0A Active CN107182943B (en) | 2017-05-18 | 2017-05-18 | A kind of method of interior mass rearing Bradysia fungus gnat |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107182943B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108990909A (en) * | 2018-05-25 | 2018-12-14 | 华南农业大学 | A kind of Bradysia fungus gnat worm living traps and the method for indoor mass rearing |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR3110338A1 (en) * | 2020-05-20 | 2021-11-26 | Fungfeed | FOOD FOR INSECTS - BREEDING / FEEDING METHOD, INSECT LARVA AND ASSOCIATED FEED |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104798734A (en) * | 2015-05-19 | 2015-07-29 | 天津市植物保护研究所 | Culture medium and method for feeding predatory coenosia larvae indoors |
| CN205667255U (en) * | 2016-06-14 | 2016-11-02 | 长江大学 | A kind of Bradysia odoriphaga adult assay device |
-
2017
- 2017-05-18 CN CN201710350446.0A patent/CN107182943B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104798734A (en) * | 2015-05-19 | 2015-07-29 | 天津市植物保护研究所 | Culture medium and method for feeding predatory coenosia larvae indoors |
| CN205667255U (en) * | 2016-06-14 | 2016-11-02 | 长江大学 | A kind of Bradysia odoriphaga adult assay device |
Non-Patent Citations (2)
| Title |
|---|
| 第19-25页: "《药用微生物技术》", 31 August 2015 * |
| 郝姗姗: "异迟眼蕈蚊对不同食用菌的嗅觉行为反应以及触角的超微结构观察", 《农业科技辑》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108990909A (en) * | 2018-05-25 | 2018-12-14 | 华南农业大学 | A kind of Bradysia fungus gnat worm living traps and the method for indoor mass rearing |
| CN108990909B (en) * | 2018-05-25 | 2020-05-15 | 华南农业大学 | Method for trapping bradysia odoriphaga live insects and feeding bradysia odoriphaga live insects indoors in large quantities |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107182943B (en) | 2019-09-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104585137B (en) | The method that field prevents and treats tobacco-plant pest-insect using fennel aphid breeding ladybug | |
| CN103155906A (en) | Aphidoletes aphidimyza mass reproduction technology and application technology | |
| CN103385220A (en) | Scale breeding method for encarsia formosa | |
| CN101569297A (en) | Breeding method of pyemotes zhonghuajia and application thereof in pest biological control | |
| CN102870742A (en) | Large-scale artificial feeding method for eretmocerus hayati | |
| CN103141443B (en) | A kind of regulate and control the method for beet webworm Ah lattice ichneumon wasp diapause | |
| CN105165734A (en) | Method for mass reproduction of Bracon adoxophyesi Mimanikawa | |
| CN104026078B (en) | A kind of reticulitermes flavipe indoor intensive raising method | |
| CN105532587B (en) | Mediterranean flour moth method for breeding and application | |
| WO2015003548A1 (en) | Method for cultivating cordyceps indoors | |
| CN105746435A (en) | Method for propagating chrysopa pallens larvae through rice moth eggs | |
| CN109769755A (en) | A kind of simple and fast propagation method of spotted wing drosophila pupa parasitic wasp hair angle clavicorn serphid | |
| CN104957107B (en) | A kind of method of interior raising Propylaea japonica | |
| CN106879557A (en) | A kind of method for raising black striped plant bug | |
| JP5929546B2 (en) | How to raise spider mite natural enemy insects | |
| CN108935341B (en) | Method for raising cabbage butterfly velvet cocoon bee | |
| CN107581159A (en) | A kind of figured cloth moths attracted by lamplight natural enemy hangs the artificial fecundation method of cocoon honeybee | |
| CN107182943B (en) | A kind of method of interior mass rearing Bradysia fungus gnat | |
| CN108323476A (en) | A kind of method of the new small peaceful mite of the double tails of artificial feeding | |
| KR101461510B1 (en) | Artificial breeding of yellow spotless Ladybugs | |
| CN104041462A (en) | Method for regulating and controlling diapause of microplitis syntretomorpha | |
| CN101984800B (en) | Method for bagged breeding of plant-feed insect | |
| CN103125451A (en) | Method for propagating Exorista civilis Rondani | |
| Abu Hassan et al. | Breeding patterns of the JE vector Culex gelidus and its insect predators in rice cultivation areas of northern peninsular Malaysia. | |
| CN111066732B (en) | Method for breeding orius sauteri |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |