CN101569297A - Breeding method of pyemotes zhonghuajia and application thereof in pest biological control - Google Patents
Breeding method of pyemotes zhonghuajia and application thereof in pest biological control Download PDFInfo
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Abstract
The invention relates to a breeding method of pyemotes zhonghuajia, comprising wild pyemotes zhonghuajia collected from persimmon smotylon japonicam lesne imago or stenhomalus taiwanus matsushita larva is propagated by using scolytus seulensis musayawa larva or pupa, stenhomalus taiwanus matsushita larva or early age of pupa as breeder seed; under the proper condition, greater wax moth larva, lasioderma serricorne larva, the early age of pupa, early age of pupa of martianus dermestoides chevrolat or gelechiid can be taken as substitutional host, and the breeder seed is inoculated into the substitutional host as stock seed; the gelechiid, sitophilus zeamais, rice weevil larva, the early age of pupa or the greater wax moth larva can be taken as substitutional host, and the stock seed and the substitutional host can be inoculated according to the proportion of 1:3; under the proper condition, the pyemotes zhonghuajia can be cultured and bred. The method has the advantages of simple operation, low production cost, being suitable for large scale batch production and long-distance transportation and the like, and the bred pyemotes zhonghuajia can be applied to the biological control of disguised and boring pests.
Description
Technical field
The invention belongs to the biological control technical field, particularly, relate to mating system of a kind of Chinese beetle Pu mite and the application in the biological control of insect pests.
Background technology
Borer pest is the crushing insect of woods fruit, and its hidden habit that causes harm has been brought very big difficulty to control, not only causes great economic loss to forestry, has also caused Ecological environment worsening simultaneously.Control to borer pest at present mainly depends on chemical control and artificial control, chemical control had both easily caused environmental pollution, be difficult to again prove effective, and many natural enemy insects was wrecked totally, cause less important insect to rise to primary pest, the wildness that causes less important insect takes place.Artificial control's efficient is low, and easily the tree body is caused artificial injury.
The method of utilizing pest natural enemy control borer pest is being sought always and inquired into to Chinese scholars for a long time, with the generation of control borer pest disaster.About long 200 μ of Pu mite (Pyemotes spp.) body, being the insect ectoparasite all, is the important biomolecule controlling elements of disguised and gregariousness insect.China beetle Pu mite (Pyemotes Zhonghuajia) belongs to pyemotes ventricosus group in the Pyemotes, it is the distinctive dominant species of China that we find when the investigation trunk borer, can parasitic multiple borer pest in the natural environment, and not only can parasitic larva, adult and ovum that also can parasitic coleopteron, be the kind of seldom seeing, application prospect is extensive, realizes that therefore large-scale industrialized the breeding of this Pu mite is to satisfy to produce the key that goes up the control borer pest.
The method of domestic existing scale breeding Pu mite, but used Pu mite kind is Pyemotes scolyhti, it is bark beetle Pu mite, this kind belongs to bark beetle Pu mite group (P.scolytigroup), its natural host scope is narrow, only parasitic bark beetle section insect, influence manually utilizes effect, several alternative host of its method employing all has weak point simultaneously: the alternative host insect source that has can't be guaranteed, as adopt the Cathay poplar roundheaded borer to breed the Pu mite, need a large amount of parasitic naturally Cathay poplar roundheaded borers of natural world of gathering, this not only is subjected to the influence of natural conditions, and the quantity of the Cathay poplar longicorn that natural world provided can't meet the demands at all; Adopt the semanotus bifasciatus larva to breed the Pu mite, the needs of scale breeding Pu mite seldom can't be satisfied in natural semanotus bifasciatus larva source, can only lean on artificial breeding to solve.It is earlier cypress to be made trap-tree that the semanotus bifasciatus larva breeds, inoculate semanotus bifasciatus with trap-tree then, again with semanotus bifasciatus larva breeding Pu mite, adopt this method to breed the Pu mite, not only need to cut down a large amount of evergreen rare species cypresses and make trap-tree, and the semanotus bifasciatus amount that is produced is little, and production scale is difficult to do greatly.The host who has does not have practical significance, as bloom first or cute conjunctivitis bee pupa because the Pu mite amount of breeding out is too little on producing.The alternative host insect source that has is subjected to market clout, and as the larva of mulberry silkworm chrysalis or honeybee, production cost is higher, or can not year-round supply, and as grub, and the field raises grub and can increase insect to environment, causes the loss of peasant on fruit tree and crops.Particularly above-mentioned alternative host is naked worm and raises, and is not easy to long-distance transport.
The Chinese beetle Pu mite that the present invention breeds out is in the newfound kind of China, does not all have relevant report both at home and abroad.Researcher of the present invention filter out gelechiid larva, rice weevil larva and just age pupa, large wax borer larva as the alternative host of mass rearing, such worm is easy to artificial a large amount of, anniversary raising, and it is with low cost, gelechiid and the rice weevil of larva perch in the cereal grain is real particularly, parasitic Chinese beetle Pu mite is subjected to the protection of a shell, can not be suitable for long-distance transport because of vibrations dead inefficacy that come off; Discharge on utensil and the method and noted waterproof and insect protected, solved the insects such as ant of going on a hunting trip in rainwater and field Chinese beetle Pu mite is discharged the loss that causes,
Summary of the invention
The mating system that the purpose of this invention is to provide Chinese beetle Pu mite.
Another object of the present invention provides the application of Chinese beetle Pu mite in biological control of insect pests.
The mating system of the present invention China beetle Pu mite (Pyemotes Zhonghuajia) comprises the steps:
1, Chinese beetle Pu mite breeder's stock is prepared: the Chinese beetle Pu mite that has wild proterties from long moth-eaten (Sinoxylonjaponicum Lesne) adult of persimmon bidentate stem or the collection of the narrow longicorn in Taiwan (Stenhomalus taiwanusMatsushit) larva body, place the dactylethrae feeder, move into wild crinosity bark beetle (Scolytidae spp.) larva or just age pupa or the narrow roundheaded borer in Taiwan or just age pupa, under 20 ℃-30 ℃ of temperature, relative moisture 50%-80%, cultivated 5-11 days, as Chinese beetle Pu mite breeder's stock.
2, the preparation of China's beetle Pu mite original seed: with greater wax moth (Galleria mellonella) larva, lasioderma serricorne (Lasioderma serricorne Fabricius) larva or just age pupa, or foreign worm (Martianus derm estoides Chevr) pupa in first age, or gelechiid (Sitotrogacerealella Oliver) larva host as an alternative, at 20 ℃-30 ℃, under the relative moisture 50%-80%, with breeder's stock and alternative host in the glass culture dish feeder according to 1: the ratio of 3-5, inoculation 8h-72h, raised 5-11 days, and cultivated and obtain Chinese beetle Pu mite original seed.
3, a large amount of breedings of Chinese beetle Pu mite: with gelechiid (Sitotroga cerealellaOliver), rice weevil (Sitophilus spp.) for example corn weevil (Sitophilus zeamaisMotschulsky), rice weevil (S.oryzae Linne) larva or just age, pupa or large wax borer larva be alternative host, Chinese beetle Pu mite original seed and alternative host are inoculated 8h-48h according to 1: 3 ratio, transfer to then in venting bags or the dactylethrae feeder, (when the host is the greater wax moth that exposes, breeding facility is a dactylethrae, and cotton makes stopper; When the host was rice weevil larva in the gelechiid larva, the grain of rice in the barley corn or pupa etc., feeder was a venting bags), be that 20 ℃-30 ℃, relative moisture are under the 50%-80% in temperature, cultivated 4-10 days, breed out Chinese beetle Pu mite.
Wherein, the described bark beetle larva of step 1 is to cut open from the peach trunk the annual 3-4 month and the 7-8 month to get, and is positioned in the culture dish, stores standby under 5-10 ℃ of condition.The narrow longicorn in described Taiwan is to cut open the 3-4 month to get in Chinese pepper branch bar, is positioned in the culture dish feeder, and is standby 5 ℃ of-10 ℃ of storages.
The described greater wax moth of step 2 is the laboratory artificial feed, raises in raising box, supports the length of time to 3-4, places 20-27 ℃ of storage standby.
Inoculation described in the step 3 is carried out in inoculation sieve, and described inoculation sieve is combined closely by the sieve at the container of bottom and top and formed, and Chinese beetle Pu mite original seed places the container of bottom, and the alternative host who is inoculated places on the sieve at top.The effect of sieve is to prevent from the host and plant the swollen abdomen body of mite to mix, and the Chinese beetle Pu mite of searching the host is passed through, and makes it find the host, and its material can be any harder materials such as plastics, stainless steel, galvanized iron sheet.Inoculator size and shape can be made as required.
The Chinese beetle Pu mite that is bred out by above-mentioned mating system preserves standby 5 ℃-18 ℃ of temperature in the refrigerating box of relative moisture 50%-80% (chamber).
When the Chinese beetle Pu mite that breeds out is transported at a distance, the dactylethrae feeder is piled up in carton, protected with foamed plastics on every side; Pile up in the carton after perhaps the venting bags feeder being sealed with sealing machine and transport.
The Chinese beetle Pu mite that is bred out by above-mentioned mating system can be applicable in the biological control of control borer pest.Described borer pest is Yang Ganxiang, semanotus bifasciatus, Cathay poplar longicorn, anoplophora glabripennis, flatheaded borer, clearwing plumage, red turpentine beetle, crinosity bark beetle, Japanese bark beetle or the little moth of Chinese scholar tree petiole etc.
The borer pest larva low age stage discharges Chinese beetle Pu mite, can effectively control Yang Ganxiang, semanotus bifasciatus, Cathay poplar longicorn, anoplophora glabripennis, flatheaded borer, clearwing plumage, red turpentine beetle, the little moth of Chinese scholar tree petiole etc. and bore the moth insect.
The present invention also provides the field method for releasing of the above-mentioned Chinese beetle Pu mite that breeds out, and it comprises the steps:
1, choose release time: concentrating on the borer pest low age stage discharges Chinese beetle Pu mite.As, when control Yang Gan resembled larva, Chinese beetle Pu mite discharged when daily mean temperature reaches 15 ℃ generally in the first tenday period of a month in late April to May; When preventing and treating other insect, be newly hatched larvae occurrence in peak period or control object emergence period release time.
2, select delivery mode: when feeder is dactylethrae, pull up tampon after, dactylethrae is covered with supporting with it gauze bag, purpose is to prevent from that other insects such as ant from crawling into to destroy the swollen abdomen body of Chinese beetle Pu mite that the mouth of pipe is oblique down, is fixed on the trunk back.
When feeder is venting bags, with sharp tweezers or other sharp devices sack is punctured the aperture of dozens of aperture 1mm, prevent that rainwater from entering, be fixed on the trunk; Or venting bags top cut off mouth, and hide rain with plastic foil, be fixed on the trunk.
3, choose burst size: the burst size of controlling Chinese beetle Pu mite the head of the tree diameter of a cross-section of a tree trunk 1.3 meters above the ground * (19000-40000)/centimetre.
Yang Gan is when resembling larva and adult in control, and the burst size of Chinese beetle Pu mite is: the tree diameter of a cross-section of a tree trunk 1.3 meters above the ground * 22000/centimetre;
When control roundheaded borer, flatheaded borer larva, clearwing plumage larva, the burst size of Chinese beetle Pu mite is: the tree diameter of a cross-section of a tree trunk 1.3 meters above the ground * 19000/centimetre;
During the little moth larvae of control petiole, the burst size of Chinese beetle Pu mite is: the tree diameter of a cross-section of a tree trunk 1.3 meters above the ground * 40000/centimetre.
In the field method for releasing of above-mentioned Chinese beetle Pu mite, can determine to discharge the position according to the controlling object harming rule.When resembling newly hatched larvae, control Yang Gan to be fixed on the high position of trunk 1 people with discharging utensil; During insects such as control longicorn, flatheaded borer, clearwing plumage, will discharge utensil to be placed on the pest damage position; During the little moth of control Chinese scholar tree petiole, be put in the trunk top near the tree crown place with discharging utensil.China beetle Pu mite is not directly shone by sunlight, is put in the trunk back.
The mating system of the present invention China beetle Pu mite has following advantage:
1, the present invention screens the alternative host as mass rearing such as gelechiid larva, rice weevil; such worm is easy to artificial a large amount of, anniversary raising; be not subjected to season and time restriction; and it is with low cost; particularly the larva perch is in the cereal grain is real; parasitic Chinese beetle Pu mite is subjected to the protection of a shell, can not be suitable for long-distance transport because of vibrations dead inefficacy that come off.
2, use the inoculation of inoculation sieve, its sieve texture improves greatly than dactylethrae and culture dish inoculation efficient.
3, noted waterproof and insect protected on release utensil and the method, helped of the protection of Chinese beetle Pu mite in the field.
4, the breeder's stock that utilizes natural host to keep Chinese beetle Pu mite helps keeping the original species of Chinese beetle Pu mite.
Embodiment
Following examples further specify content of the present invention, but should not be construed as limitation of the present invention.
Breeding of embodiment 1 Chinese beetle Pu mite breeder's stock
Will be from the long moth-eaten mature larva that becomes Chinese beetle Pu mite that polypide gathers and wild crinosity bark beetle of persimmon bidentate stem (or first age pupa) with wild proterties, (specification is long 7cm to place the dactylethrae feeder, the glass tube of internal diameter 2cm) in, in 27 ± 2 ℃, relative moisture 50%-80%, well-ventilated, clean dustless environment, inoculation, with the cotton plug jam-pack mouth of pipe, cultivated 7 days, the Chinese beetle Pu mite offspring who breeds is as Pu mite breeder's stock.
Embodiment 2 utilizes the greater wax moth larva to produce Chinese beetle Pu mite original seed for substituting the host
Present embodiment selects for use the greater wax moth larva of artificial feeding as the alternative host who produces Chinese beetle Pu mite original seed.Select the 3-4 greater wax moth larva in age, place culture dish, the tampon that the glass dactylethrae of Pu mite breeder's stock is housed is pulled out, also place culture dish, 27 ± 2 ℃ of temperature, under the relative moisture 50%-80% condition, with breeder's stock and alternative host in glass culture dish feeder (diameter 15cm glass culture dish) according to 1: 3 ratio, behind the inoculation 8h, change in the dactylethrae feeder, raise 7 days to new Chinese beetle Pu mite generation, promptly can be used as Chinese beetle Pu mite and produce original seed.
It is to substitute the host to produce Chinese beetle Pu mite original seed that embodiment 3 utilizes lasioderma serricorne
Select lasioderma serricorne pupa in first age as the alternative host who produces Chinese beetle Pu mite original seed, to substitute host's lasioderma serricorne pupa and place culture dish, the glass dactylethrae cotton plug that Chinese beetle Pu mite breeder's stock will be housed simultaneously pulls out, also place same culture dish, at 27 ± 2 ℃, relative moisture 70%-80%, the well-ventilated, under the clean dustless environment, with breeder's stock and alternative host in the glass culture dish feeder according to 1: 5 ratio, behind the inoculation 8h, change in the culture dish feeder (diameter 15cm glass culture dish), raise 7 days to new Chinese beetle Pu mite generation, promptly can be used as Chinese beetle Pu mite original seed.
Embodiment 4 utilizes the gelechiid larva to carry out a large amount of breedings of Chinese beetle Pu mite for substituting the host
1, prepares to substitute the host
Artificial feeding gelechiid larva after the gelechiid larvae development is abundant, promptly can be used as Chinese beetle Pu mite and substitutes host's use.
2, inoculation
The preparation of inoculation sieve: the inoculation sieve was made by stainless steel by two-layer composition up and down, and lower floor is the hydrostatic column of diameter 20cm, high 0.5cm; The upper strata is a sieve, 60 order stainless (steel) wires; In conjunction with tight, Chinese beetle Pu mite original seed is placed by lower floor between the levels, and the upper strata holds the alternative host who is inoculated.
At 27 ± 2 ℃, relative moisture 50-80%, under well-ventilated, the clean dustless environment, Chinese beetle Pu mite original seed is put in the container of inoculation sieve lower floor, the alternative host gelechiid that raising is obtained is placed on the sieve on inoculation sieve upper strata simultaneously, inoculative proportion is original seed: alternative host=1: 3, inoculation 24h.
3, Chinese beetle Pu mite is cultivated
To finish the gelechiid of inoculation, transfer in the venting bags, is that 27 ± 2 ℃, relative moisture are under 50%-80%, well-ventilated, the clean dustless environment in temperature, cultivates 7 days, breeds out the Chinese beetle Pu mite that is used for field release.If (transport for long-distance then cultivated 5 days.)
4, Chinese beetle Pu mite preserves
Seal and be kept at 10 ℃-18 ℃ of temperature the venting bags (be growth 10cm by nonwoven, wide 8cm sack seals with sealing machine) of cultivating good Chinese beetle Pu mite is housed, in the refrigerating box of relative moisture 50%-80% (chamber).
5, Chinese beetle Pu mite transportation
Take out from refrigerating box cultured Chinese beetle Pu mite venting bags is housed, pile up in the transportation incubator, transportation incubator temperature is controlled at 10 ℃-15 ℃.
Embodiment 5 utilizes the greater wax moth larva to carry out a large amount of breedings of Chinese beetle Pu mite for substituting the host
1, prepares to substitute the host
In the insectary greater wax moth was supported to the 3-4 instar larvae stage, standby.
2, inoculation
The preparation of inoculation sieve: the inoculation sieve is made up of two-layer hydrostatic column up and down, is made by stainless steel, and lower floor is the hydrostatic column of diameter 20cm, high 0.5cm; The upper strata is a sieve, 60 order stainless (steel) wires; The upper strata is seated at lower floor as food steamer.
At 27 ± 2 ℃, about relative moisture 50%, under well-ventilated, the clean dustless environment, Chinese beetle Pu mite original seed is placed the container of inoculation sieve lower floor, alternative host's greater wax moth larva that raising is obtained is placed on the sieve on inoculation sieve upper strata simultaneously, inoculative proportion is original seed: alternative host=1: 3, inoculation 8h.
3, Chinese beetle Pu mite is cultivated
The greater wax moth larva that will be fixed with Chinese beetle Pu mite after the inoculation is transferred to glass dactylethrae (specification: long 7cm, internal diameter 2cm) in, each dactylethrae is put 20-25 greater wax moth larva, clog the mouth of pipe with cotton, pile up in the carton case, place under 27 ± 2 ℃, relative moisture 50%, clean dustless, the draughty environmental condition and cultivated 7 days, breed out and be used for the Chinese beetle Pu mite that the field discharges.If (transport for long-distance then cultivated 5 days.)
4, Chinese beetle Pu mite preserves
Being housed, the carton case of cultivating good Chinese beetle Pu mite is kept at 10 ℃-18 ℃ of temperature, in the refrigerating box of relative moisture about 50%.
5, Chinese beetle Pu mite transportation
Take out from refrigerating box cultured Chinese beetle Pu mite carton case is housed, be put in the transportation incubator, on every side with the foamed plastics protection, transportation incubator temperature is controlled at 10 ℃-15 ℃.
Embodiment 6 Chinese beetle Pu mites are applied to prevent and treat forestry quarantine property insect Yang Gan and resemble
1, release time
The control Yang Ganxiang larva that survives the winter discharges the Chinese beetle Pu mite time in the first tenday period of a month in late April to May, discharges when daily mean temperature reaches 15 ℃.
2, method for releasing
Adopt 1 property submerged to discharge.
Rubber band is tied to the high position of trunk 1 people, the tampon of feeder dactylethrae is pulled up, cover dactylethrae with corresponding gauze bag, the mouth of pipe is oblique down, utilizes rubber band to be fixed in the trunk back (comprising cotton plug).
3, burst size
Present embodiment adopts individual plant control formula to discharge, and the computing formula of Chinese beetle Pu mite burst size is: the tree diameter of a cross-section of a tree trunk 1.3 meters above the ground * 22000/centimetre.
Discharge Chinese beetle Pu acarid control Yang Gan and resemble the larva that survives the winter, discharge back 21d investigation, corrected mortality is 91.74%.Effectively control Yang Ganxiang harm by discharging Chinese beetle Pu mite, reached the purpose that protects trees.
Embodiment 7 Chinese beetle Pu mites are applied to prevent and treat the little moth of Chinese scholar tree petiole
1, release time
The little moth larvae harm phase of petiole discharges.
2, method for releasing
During release the feeder venting bags is pricked out the dozens of aperture with sharp tweezers or other sharp utensils on sack, about hole diameter 1mm, then venting bags is tied to the trunk top near the tree crown place with rubber band.
3, burst size
Present embodiment adopts individual plant control formula to discharge, and the computing formula of Chinese beetle Pu mite burst size is: the tree diameter of a cross-section of a tree trunk 1.3 meters above the ground * 40000/centimetre.
Discharge the little moth larvae of Chinese beetle Pu mite control petiole, discharge back 12 days, Chinese beetle Pu mite is to the parasitic rate average out to 94.23% of the little moth larvae of petiole.Effectively control causing harm of the little moth of Chinese scholar tree petiole by discharging Chinese beetle Pu mite, reached the purpose that protects trees.
Embodiment 8 Chinese beetle Pu mites are applied to prevent and treat anoplophora glabripennis
1, release time
Discharge in the anoplophora glabripennis larva hatching peak phase.
2, method for releasing
During release the feeder venting bags is pricked out the dozens of aperture with sharp tweezers or other sharp utensils on sack, about hole diameter 1mm, then venting bags is tied to trunk pest harm position with cotton rope.
3, burst size
Present embodiment adopts individual plant control formula to discharge, and the computing formula of Chinese beetle Pu mite burst size is: the tree diameter of a cross-section of a tree trunk 1.3 meters above the ground * 19000/centimetre.
At anoplophora glabripennis newly hatched larvae occurrence in peak period, calculate Chinese beetle Pu mite quantity Pest Control according to Chinese beetle Pu mite burst size formula, control efficiency reaches 98.93%.Effectively control causing harm of anoplophora glabripennis by discharging Chinese beetle Pu mite, reached the purpose that protects trees.
Claims (9)
1, the mating system of Chinese beetle Pu mite is characterized in that, comprises the steps:
(1) preparation of Chinese beetle Pu mite breeder's stock: gather Chinese beetle Pu mite with wild proterties from long moth-eaten adult of persimmon bidentate stem or the narrow roundheaded borer body in Taiwan, place the dactylethrae feeder, move into wild crinosity bark beetle larva or just age pupa or the narrow roundheaded borer in Taiwan or just age pupa, under 20 ℃-30 ℃ of temperature, relative moisture 50%-80%, cultivated 5-11 days, as Chinese beetle Pu mite breeder's stock;
(2) preparation of Chinese beetle Pu mite original seed: with greater wax moth larva or lasioderma serricorne larva or first first age pupa or the gelechiid host as an alternative of age pupa or foreign worm, under 20 ℃-30 ℃, relative moisture 50%-80%, with breeder's stock and alternative host in the glass culture dish feeder according to 1: the ratio inoculation 8h-72h of 3-5, raised 5-11 days, and cultivated and obtain Chinese beetle Pu mite original seed;
(3) a large amount of breedings of Chinese beetle Pu mite: with gelechiid, corn weevil, rice weevil larva or just age, pupa or large wax borer larva be alternative host, Chinese beetle Pu mite original seed and alternative host are inoculated 8h-48h according to 1: 3 ratio, transfer to then in venting bags or the dactylethrae feeder, in temperature is that 20 ℃-30 ℃, relative moisture are under the 50%-80%, cultivated 4-10 days, and bred out Chinese beetle Pu mite.
2, mating system as claimed in claim 1 is characterized in that, the described bark beetle larva of step (1) is to cut open from the peach trunk the annual 3-4 month and the 7-8 month to get, and is positioned in the culture dish, stores standby under 5-10 ℃ of condition; The narrow longicorn in described Taiwan is to cut open the 3-4 month to get in Chinese pepper branch bar, is positioned in the culture dish, and is standby 5-10 ℃ of storage;
The described greater wax moth of step (2) is the laboratory artificial feed, raises in raising box, supports the length of time to 3-4, places 20-27 ℃ of storage standby.
3, mating system as claimed in claim 1, it is characterized in that, inoculation described in the step (3) is carried out in the inoculation sieve, described inoculation sieve is combined closely by the sieve at the container of bottom and top and is formed, China's beetle Pu mite original seed places the container of bottom, and the alternative host who is inoculated places on the sieve at top.
As the arbitrary described mating system of claim 1-3, it is characterized in that 4, the Chinese beetle Pu mite that breeds out preserves standby 5 ℃-18 ℃ of temperature in the refrigerating box of relative moisture 50%-80%.
5, the application of Chinese beetle Pu mite in the biological control of control borer pest of using the arbitrary described mating system of claim 1-4 to breed out.
6, application as claimed in claim 5 is characterized in that, described borer pest is Yang Ganxiang, semanotus bifasciatus, Cathay poplar longicorn, anoplophora glabripennis, flatheaded borer, red turpentine beetle, crinosity bark beetle, Japanese bark beetle, clearwing plumage or the little moth of Chinese scholar tree petiole.
7, use the field method for releasing of the Chinese beetle Pu mite that the arbitrary described mating system of claim 1-4 breeds out, it is characterized in that, comprise the steps:
(1) choose release time: concentrating on the borer pest low age stage discharges Chinese beetle Pu mite;
(2) select delivery mode: when feeder is dactylethrae, pull up tampon after, dactylethrae is covered with the gauze bag, oblique time of the mouth of pipe is fixed on the trunk back;
When feeder is venting bags, sack is punctured the aperture of dozens of aperture 1mm, be fixed on the trunk, or venting bags top is cut off, hide with plastic foil, be fixed on the trunk.
(3) choose burst size: the burst size of controlling Chinese beetle Pu mite the head of the tree diameter of a cross-section of a tree trunk 1.3 meters above the ground * (19000-40000)/centimetre.
8, field as claimed in claim 7 method for releasing is characterized in that, in the step (1), when control Yang Gan resembled larva, Chinese beetle Pu mite discharged when daily mean temperature reaches 15 ℃ generally in the first tenday period of a month in late April to May; When preventing and treating other insect, be newly hatched larvae occurrence in peak period or control object emergence period release time.
9, as claim 7 or 8 described field method for releasing, it is characterized in that, in the step (2), will be fixed on the high position of trunk 1 people with discharging utensil when control Yang Gan resembles newly hatched larvae; When control longicorn, flatheaded borer or clearwing plumage insect, will discharge utensil and be placed on the pest damage position; During the little moth of control Chinese scholar tree petiole, place the trunk top near the tree crown place with discharging utensil.
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| CN102812928A (en) * | 2012-08-02 | 2012-12-12 | 北京林业大学 | Breeding method of pyemotes tritici |
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