JP5929546B2 - How to raise spider mite natural enemy insects - Google Patents

Description

  The present invention relates to a natural enemy insect breeding method and breeding container capable of efficiently and massively raising spider mite natural enemy insects.

  Spider mites have become a problem around the world as important pests that cause serious damage to crops such as vegetables, fruit trees, and flowers. Spider mites are difficult-to-control pests that develop a high level of drug resistance. Therefore, biological control using natural enemy organisms is effective.

  Possible natural enemies of spider mites include spider mites (such as dust mites) and natural enemy insects (such as poppy, nematode, red thrips, spider mites, etc.). In order to efficiently breed and propagate these natural enemy organisms, it is necessary to appropriately combine spider mites that serve as natural enemies and host plants that serve as spider mites.

  Cabbular mites generally do not require a large number of prey mites for growth and egg production, and are relatively easy to breed. Therefore, a large-scale propagation method using a kidney bean or a lima bean that is parasitic on a spider mite has been developed. Patent Document 1 and Non-Patent Document 2). However, red mites are vulnerable to pesticides and have problems such as being unable to exert their control effect when spider mites occur frequently.

  On the other hand, natural enemy insects are relatively strong against pesticides and exert their control effect when spider mites occur frequently. Therefore, an efficient and large-scale method for rearing natural enemy insects is eagerly desired. However, the breeding methods that have been developed are limited to small-scale breeding methods in which petals and the like that are parasitized by the spider mite are given to a small number of natural enemy insects in petri dishes (Non-patent Documents 3 and 4).

Overmeer, W. P. J. 1985. Rearing and handling. In Spider Mites: Their Biology, Natural Enemies and Control. Vol. 1B (W. Helle and M. W. Sabelis eds.). Elsevier, Amsterdam, pp. 161-170. Shih, C.I.T., 2001. Automatic mass-rearing of Amblyseius womersleyi (Acari: Phytoseiidae). Experimental and Applied Acarology 25, 425-440. Kishimoto, H., 2003. Development and oviposition of predacious insects, Stethorusjaponicus (Coleoptera: Coccinellidae), Oligota kashmirica benefica (Coleoptera: Staphylinidae), and Scolothrips takahashii (Thysanoptera: Thripidae) reared on different spider mite Teite Applied entomology and zoology 38, 15-21. Takeshi Shimoda, 2004. Breeding of spider mite natural enemy insect poppy seeds. Plant protection 58 (12) 545-548 Hiroshi Amano, 1996. Chapter 6 Experimental methods, Plant mite studies (Shozo Ehara and Tokujun Shinji), National Rural Education Association p.314-322.

  In the conventional small-scale breeding method, the number of natural enemy insects that can be reared is as few as several tens per container. Also, depending on the type of natural enemy insect, breeding conditions differ for each growth stage. For this reason, the mainstream is a method in which each breeding stage is isolated and housed in a separate container and the feed is replenished frequently (every 2-3 days). However, such a breeding method is difficult, and there is a problem that not only high technology and experience but also a great effort is required (Non-Patent Document 5).

  However, a large-scale breeding method that can replace the small-scale breeding method has not yet been developed. Raising spider mites natural enemies is much more difficult than raising spider mites. One reason is that natural enemy insects require a greater amount of prey mites compared to red mites. The other reason is due to the difference between ticks and natural enemy insects. For example, when the growth method using a common bean mite that is infested with a spider mite, which has been established as a method for mass growth of red mites, is applied to natural enemy insects, natural enemy insects may die in large quantities. The main reason for this is that natural enemy insects get caught in the hair flies on the leaves of kidney beans and die.

  In addition, for some natural enemy insects such as spider fly, even a small breeding method has not been developed.

  The present invention has been made in view of the above problems, and an object of the present invention is to provide a natural enemy insect breeding method and the like capable of efficiently and massively breeding spider mite natural enemy insects.

  The inventors of the present application have made extensive studies to solve the above problems. First, in order to reduce the labor of frequent supplementation of spider mites, we tried to use various types of live plants infested with spider mites for breeding natural enemy insects. As a result of the trial, it is necessary to select live plants that can infest parasitic mites in a larger amount and sustainably better than natural mite insects for breeding natural enemy insects, and have excellent compatibility with natural enemy insects. There was found. For example, leguminous plants such as kidney beans are typical examples of spider mite-like plants, but when they parasitize in large quantities, they will fall, and natural enemy insects may be caught and killed by hair flies on the leaves. It turned out to be rather unsuitable for breeding natural enemy insects.

  In addition, since natural enemy insects are bred in a breeding container, it is necessary to select a live plant suitable for growing in the breeding container.

  As a result of these intensive studies, the present inventors have found that the above problem can be solved by using at least one kind of vegetable selected from the group consisting of Brassicaceae, Asteraceae, and Rabbitaceae, and arrive at the present invention. It came to.

  That is, the natural enemy insect breeding method according to the present invention is a pot plant which is at least one kind of vegetable selected from the group consisting of a breeding process for propagating spider mites, and a cruciferous, asteraceae and rhesus family. A parasitic process for infesting the grown vegetables with the proliferated spider mites, and a breeding process for placing the pot-planted vegetables infested with the mites in a breeding container and breeding natural enemies of spider mites in the breeding container; It is characterized by including.

  In the breeding method according to the present invention, in the above method, the vegetable is preferably a cruciferous vegetable.

  In the breeding method according to the present invention, in the above method, the vegetable is more preferably Komatsuna.

  The breeding method according to the present invention uses the at least one plant selected from the group consisting of leguminous, cruciferous, cucurbitaceae, solanaceous, rose, and convolvulaceae plants in the propagation step. Is preferably grown.

  In the breeding method according to the present invention, it is more preferable to grow the spider mites using leguminous plants in the growth step.

  In the breeding method according to the present invention, it is more preferable that the spider mites are propagated using kidney beans in the propagation step.

  In the breeding method according to the present invention, the plant is preferably cultivated in an indoor environment in the growth step.

  In the breeding method according to the present invention, in the above method, the spider mite is preferably a tick belonging to the genus Spider mite.

  In the breeding method according to the present invention, in the above method, the spider mite is more preferably a spider mite.

  In the breeding method according to the present invention, in the method described above, the natural enemy insect is preferably at least one selected from the group consisting of Poppy beetles, ladybirds, thrips, and flies.

  In the breeding method according to the present invention, in the method described above, the natural enemy insect is more preferably a spider mite, a spider mite, a spider mite, a red spider, or a spider fly.

  The present invention is also a breeding container used for the breeding method, which covers a cylindrical container having a bottom surface and an upper surface, and an opening formed on the upper surface, and has an eye size of 0.01 mm or more and 0.1 mm. The above-mentioned cylindrical container is formed by detachably joining a cylindrical lower part having the bottom surface and a cylindrical upper part having the upper surface, An upper part is provided with the engagement protrusion which contact | abuts the inner surface of the said lower part in the said joining, The said upper part is translucent, The breeding container characterized by the above-mentioned is provided.

  According to the present invention, since at least one kind of vegetable selected from the group consisting of pot-planted Brassicaceae, Asteraceae, and Rabbitaceae is used, breeding that can raise spider mite natural enemy insects efficiently and in large quantities There is an effect that a method and the like can be provided.

(A) is a figure which shows an example of the breeding container based on this invention, (b) is an upper cylinder part (upper part) and lower cylinder part (lower part) of the breeding container used for breeding of natural enemy insects in the said breeding container FIG. (A) And (b) is a figure which shows a mode that a nymph mite is bred in large quantities in an Example, (c) is a figure which shows a pot-spotted nymph mite parasitic Komatsuna, (d), (e), (G) And (h) is a figure which shows natural enemy insects, (f), (i) and (k) are figures which show the mode after natural enemy insect introduction in an Example. (J) is a figure which shows the mode of the natural enemy insect which ingests this with respect to bait | feeds, such as a honey liquid applied to the internal wall surface of the upper cylinder part 1 of a breeding container in an Example. In one Example, it is a figure which shows the growth pattern of the yellow-spotted beetle. In another Example, it is a figure which shows the growth rate of a common yellow-spotted beetle. In another Example, it is a figure which shows the growth rate of a common yellow-spotted beetle. In a reference example, it is a figure which shows the survival rate of the sand-bearing thrips at the time of using a kidney bean leaf piece. (A) is a figure which shows a waste leaf piece in a reference example, (b) is a figure which shows the survival rate of the sand-bearing thrips at the time of using a waste leaf piece in a reference example.

[1. (How to raise natural enemy insects)
(Overview)
The natural enemy insect breeding method according to the present invention includes a step of growing spider mites, and at least one kind of vegetable selected from the group consisting of cruciferous, asteraceae, and rhododendron vegetables, and is potted vegetable A parasitic step of infesting the grown spider mites, and a rearing step of placing the pot-planted vegetables infested with the spider mites in a rearing container and rearing the natural enemy insect of the spider mite in the rearing container. This is a method of raising natural enemy insects.

(Advantages of the present invention)
1) The present invention uses the above-mentioned vegetables that can be easily cultivated indoors or in a greenhouse as a plant for raising natural enemy insects, so that natural enemy insects can be raised throughout the year. Moreover, advanced knowledge and skills are not required for breeding natural enemy insects.
2) Since the said vegetable as a host plant which infests spider mites is used in the state of the pot plant raw plant body, compared with the conventional method using a leaf piece etc., a plant does not deteriorate easily. In addition, since the host plant used in the present invention hardly affects the plant body even if a large amount of spider mites are infested, a large number of spider mites can be maintained on the plant body for a long period of time. It is not necessary to replenish and replace spider mites) frequently. Moreover, since these host plants are used in the state of pot planting, they can be easily exchanged.
3) Since the above-mentioned vegetables can maintain a large number of foods (spider mites), problems such as developmental inhibition of natural enemy insects, decrease in the number of eggs laid, and starvation due to food shortage do not occur, and natural enemy insects and larvae are isolated. There is no need to rear them. In addition, since these vegetables are compatible with various types of natural enemy insects, a large amount of natural enemy insects (hundreds of animals per breeding container) can be reared.

  By having these advantages, a large number of natural enemy insects can be efficiently raised with less effort than conventional methods, and it is possible to handle various types of natural enemy insects throughout the year. it can. Furthermore, since natural enemy insects can be bred in succession, a large number of natural enemy insects can be obtained as a result.

  First, natural enemy insects, spider mites and plants related to the present invention will be described.

(Natural enemy insect)
In the present invention, natural enemy insects refer to insects that prey on spider mites. Natural enemy insects include, for example, poppy beetles, ladybirds, thrips, wing flies (Feltiella spp., Anthrocnodax spp., Therodiplosis spp.), Bugs (Orius spp.), Geocoris spp., Etc. Is mentioned. More specifically, Japanese indigenous natural enemies include Oligota kashmirica benefica, Oligota yasumatsui, Stethorus japonicus, Scolohrips takahashii, Examples include Feltiella acarivora. Other foreign enemies include Oligota flavicornis, Oligota oviformis, Stethorus punctum, Stethorus punctillum, Stethorus bifidus, Stethorus nigripes, Scolohrips sexmaculatus, Scolothrips indicues, Feltiella sp., Anococnoda peri .

(Spider mites)
In the present invention, spider mites that are food for natural enemy insects refer to ticks belonging to the family Tetranychidae on taxonomy. As the spider mites, mites belonging to the genus Nite spider mite (genus Tetranychus) are preferable. Examples of the genus Nite spider mite include Tetranychus urticae Koch, Kantawa spider mite (Tetranychus kanzawaiKishida), Sagaminari spider mite (Tetranychus phaselusEhara), Ashinowa spider mite (Tetranychus ludeniZacher), Nari spp. Among them, the spider mite that is a food for many kinds of natural enemy insects is preferable.

(Tick mite propagation plant)
In the growth step, it is preferable to use a plant for growing spider mites. The plant used here (spotting plant for spider mites) may be any plant that can feed on spider mites and can grow spider mites, such as legumes, Brassicaceae, Cucurbitaceae, eggplants, roses, convolvulaceae, etc. Plant. More specifically, legumes include common bean (Phaseolus vulgaris), lima bean (Phaseolus lunatus), cowpea (Phaseolus unguiculata), soybean (Glycine max), peanut (Arachis. Hypogaea), peanut (Glycine soja), pea. (Pisum. Sativum), Kudu (Pueraria lobata (Wild) Ohwi), and the like. Examples of the cruciferous plants include Komatsuna (Brassica rapa var. Perviridis), Chingensai (Brassica rapa var. Chinensis), Japanese radish (Raphanus sativus L.), Chinese cabbage (Brassica rapa var. Pekinensis), and the like. Examples of cucurbitaceae plants include cucumber (Cucumis sativus) and pumpkin (Cucurbita spp.). Examples of solanaceous plants include eggplant (Solanum melongena), tomato (Solanum lycopersicum), and the like. Examples of the Rosaceae plants include strawberry (Fragaria L.), rose (Rosa spp.), Pear (Pyrus pyrifolia var. Pyrifolia), apple (Malus pumila), cherry (Prunus subg. Cerasus), and the like. Examples of the convolvulaceae plant include Ensai (Ipomoea aquatica). The plants exemplified above are particularly preferred for spider mites and have the common property that the total leaf area per individual is relatively large. Among these, leguminous plants, in addition to these characteristics, have the characteristics that they reach the total leaf area in a relatively short period of time and are relatively easy to grow. ) Is preferable because it easily grows in large quantities.

  As a plant for spider mite propagation, a plant individual (live plant) may be used as it is for the growth of spider mites, or a part of the plant individual (for example, a leaf piece or the like) may be used for the growth of spider mites. The individual is used as it is for the growth of spider mites.

  It is more preferable to use a plant that can be easily cultivated throughout the year in a room, a greenhouse, or the like, and that can easily grow spider mites, as a spider mite propagation plant, because a large amount of spider mites can be grown throughout the year. Among such plants, kidney beans, lima beans, cowpeas, soybeans, peanuts, wild beans, and peas are particularly preferable among the above examples. For example, kidney beans are easy to grow indoors and grow fast, and when grown at around 23 ° C., they grow to a size that can be used for mass growth of spider mites in about two weeks after sowing. Although not particularly limited, in the case of kidney beans, the plant height is preferably about 30 cm or more as a size that can be used for mass growth of spider mites.

  The cultivation method may be determined appropriately depending on the type of plant. For example, in the case of kidney beans, about 1 to 10 seeds are sown in a pot containing culture soil, placed in a vat containing water, and grown in a temperature-controlled room (23 ± 2 ° C., 1000-3500 lux, 16L8D). To do. What is necessary is just to use the plant body cultivated for about 2 to 3 weeks after sowing for mass growth of spider mites.

(Plant for raising natural enemy insects)
The plant used as a place for breeding natural enemy insects used in the parasitic process and breeding process (hereinafter referred to as a plant for breeding natural enemy insects) is at least one selected from the group consisting of cruciferous, asteraceae and red-breasted vegetables. It is a vegetable planted in a pot. These plants can be easily cultivated 1) indoors or in greenhouses, etc. 2) can maintain a large amount of spider mites in a pot planted state 3) can easily breed many natural enemy insects in a pot planted state 4) There is a common advantage that it can be commonly used for breeding multiple types of natural enemy insects.

  Unlike natural plants such as legumes, the above natural enemy insect-raising plants rarely fall off even if they are subjected to feeding stress caused by spider mites. Therefore, even when a large number of spider mites are inoculated, spider mites and natural enemy insects can be maintained over a long period of time. In addition, since there is almost no fallen leaves, there is relatively little risk of soiling the breeding container. In addition, unlike common bean and the like, there are no key-like hair flaws on the surface of the leaves, so natural enemy insects will not be caught and die.

  The category of plants for raising natural enemy insects excludes wild grasses that are not used as vegetables, that is, edible plants. As a result of artificial selection and improvement, for example, vegetables have characteristics such as easy cultivation and large growth of edible parts in a relatively short period of time. More suitable for cultivation. In addition, among vegetables, leafy vegetables with leaves as edible parts are mainly used from the viewpoint that spider mites are able to infest a large leaf area and that the leaves are relatively soft and spider mites prefer to infest. More preferred. The radish and turnip, which will be described later, are root vegetables, but can be used in the same way as leaf vegetables in that the growth at the initial stage is exclusively spent on the development of the leaves.

  The cruciferous vegetables include Komatsuna, Chingensai, Japanese radish, Chinese cabbage, Mizuna (Brassica rapa var. Lancinifolia), turnip (Brassica rapa L var. Rapa, Brassica rapa L var. Glabra), cabbage (Brassica oleracea var. Capitata), Broccoli (Brassica oleracea var. Italica), watercress (Nasturtium officinale), Takana (Brassica juncea var. Integlifolia), Nozawana (Brassica rapa var. Hakabura), and the like. Asteraceae vegetables include lettuce (Lactuca sativa), shungiku (Glebionis coronaria), trevis (Cichorium intybus var. Foliosum), and the like. Examples of the red crustacean vegetables include spinach (Spinacia oleracea). Among them, cruciferous vegetables that are compatible with more types of natural enemy insects are preferable, cruciferous leafy vegetables are more preferable, komatsuna or chingensai are more preferable, and komatsuna are particularly preferable. A plurality of kinds of natural enemy insect rearing plants can be used in combination.

  Plants for raising natural enemy insects are grown in pots. Here, the pot may be a cultivation container that can accommodate a natural enemy insect-raising plant and a medium, and the size, shape, and the like are not particularly limited. In addition, when the example of a culture medium is given, in the case of hydroponics, it is water or a culture medium for hydroponics, and in non-hydroponics (cultivation using a solid culture medium), it is soil or a solid soil substitute. Examples of solid soil substitutes include vermiculite and peat moss.

  The cultivation method may be determined appropriately depending on the type of plant for raising natural enemy insects. Taking the case of Komatsuna as an example, about 1 to 10 Komatsuna seeds are sown in a pot (diameter 9 cm) containing culture soil, placed in a vat containing water, and placed in a temperature-controlled room (23 ± 2 ° C., 8000). Cultivated for about 2 weeks at 10000 lux, 16L8D), and further cultivated in a greenhouse (23 ± 3 ° C.) for about 3 to 4 weeks. By this cultivation method, it is possible to obtain a strong plant necessary for maintaining natural enemy insects over a relatively long period in a state inoculated with a large number of spider mites. Considering the effects on the survival and growth of natural enemy insects and the damage stress caused by many inoculated spider mites, Komatsuna can be cultivated in a constant temperature greenhouse of the above conditions for about 2 weeks and then in a greenhouse of the above conditions. For example, a plant body of about 3 to 8 weeks after sowing is preferable for breeding natural enemy insects, and a plant body of 5 to 6 weeks after sowing is most preferable. Although not particularly limited, in the case of Komatsuna, the plant height is preferably about 20 cm or more as a size that can be used for the growth of natural enemy insects.

  Next, each step of the breeding method according to the present invention will be described.

(Proliferation process)
The growth process for growing spider mites will be described. The method for propagating spider mites in the propagation step is not particularly limited, but it is preferable that the spider mites are propagated by infesting the spider mite propagation plant grown to a predetermined size.

  The spider mite propagation plant used in the propagation step and the spider mite propagation plant in the propagation step may be cultivated outdoors, but are preferably cultivated in an indoor environment isolated from the outside, such as indoors or greenhouses. The reason is that it is possible to stably supply spider mites propagation plants and spider mites throughout the year, and to reduce the risk of contamination such as contamination with mites.

  In a more specific example, several leaves of a spider parasitic plant are placed on a plant body of a spider mite propagation plant grown to a predetermined size, and 2 to 2 in another temperature-controlled room (for example, 23 ± 2 ° C., 16L8D). Next-generation individuals of spider mites are obtained by keeping them isolated for 3 weeks. By repeating this procedure, spider mites can be efficiently grown in large quantities.

(Parasitic process)
A parasitic process for parasiticizing the above-grown spider mites on pot-planted vegetables (plants for natural enemy insect breeding) will be described. The parasitic process is performed by cultivating the natural enemy insect breeding plant after moving the spider mites that have been propagated onto the natural enemy insect breeding plant grown to a predetermined size. The method for moving spider mites onto the natural enemy insect-raising plant is not particularly limited.

  The natural enemy insect rearing plant used in the parasitic process and the natural enemy insect rearing plant in the parasitic process may be cultivated outdoors, but are preferably cultivated in an indoor environment isolated from the outside, such as indoors or greenhouses. The reason is that it becomes possible to stably supply natural enemy insect-raising plants and spider mites throughout the year, and to reduce the risk of contamination such as contamination with mites.

  In addition, the parasitic process can be performed simultaneously with the following breeding process, but it is preferable to carry out the breeding process after causing a sufficient amount of spider mites to infest the natural enemy insect breeding plant.

  In a more specific example, first, a pot-planted natural enemy insect breeding plant grown to a predetermined size is placed in a bat filled with water. Next, several leaves of the above-mentioned spider mite-growing plant on which the spider mites have been propagated are placed on the plant body of the natural enemy insect breeding plant, and 1 in a constant temperature room (for example, 25 ± 1 ° C., 2000-5000 lux, 16L8D). After maintaining for about 3 days, the leaves of the dead mite-growing plant are removed. Thereby, the natural enemy insect breeding plant in the state where many spider mites (for example, 100 to 400 female adults per pot, 5 to 10 times the total growth stage) are infested can be obtained.

(Breeding process)
The rearing process for rearing spider mite natural enemy insects will be described. The breeding step is a step of placing a pot-planted natural enemy insect breeding plant infested with ticks into a breeding container for breeding natural enemy insects, and breeding the natural enemy insects of spider mites in the breeding container.

  In the breeding process, water necessary for the growth of natural enemy insect breeding plants is supplied as necessary. In addition, when the natural enemy insect rearing plant is dead, the pot planted natural enemy insect rearing plant is replaced as necessary. The breeding process may be performed outdoors, but in order to be able to stably supply natural enemy insects throughout the year, it is preferably performed in an indoor environment isolated from the outside, such as indoors and greenhouses.

  The breeding container for breeding natural enemy insects 1) has an internal space that can accommodate pot-planted natural enemy insect breeding plants, 2) can be kept isolated so that spider mites and natural enemy insects do not escape, and 3) What is necessary is just to have an openable / closable structure in which pot planted natural enemy insect breeding plants and natural enemy insects can be taken in and out, and 4) at least a ventilable structure (aeration structure). When the light source is installed outside the breeding container, the breeding container is preferably translucent. For example, a breeding container (FIG. 1 (a)) described below can be used (see also FIG. 2 (f) and FIG. 2 (i)).

  In order to satisfy the condition 2) more reliably, it is preferable not to have a structure that always communicates the inside and outside of the breeding container other than the ventilation structure 4). In order to continuously perform the breeding process, the breeding container may preferably have an internal space that can receive a plurality of pots planted with natural enemy insect breeding plants.

  In a more specific example of the breeding process, first, about 1-2 pots of natural enemy insect breeding plants infested with ticks are placed in the breeding container, and the natural enemy insects are introduced. After introduction of natural enemy insects, add 1-2 pots of natural enemy insect breeding plants infested with mites every 7 to 10 days according to the amount of food in the breeding container, and remove old pots as necessary ( After replacement, water is supplied every few days. As for poppy seeds, adults, eggs and larvae live on plants (FIG. 2 (g)), whereas moths need to form cocoons in the soil and develop (FIG. 2 (h)). ), Lay about 5 cm of moist vermiculite on the bottom of the breeding container (FIG. 2 (i)), and install a plant pot for raising natural enemy insects. In addition, when a pot is installed directly on vermiculite, pressure is applied to the poppy seed beetle in the vermiculite due to the weight of the pot and it dies, so a circular net or the like (pressure distribution means) is installed between the two to distribute the pressure. It is more preferable that As for spider mites, larvae are predatory on spider mites, whereas adults feed on nectar, etc., so that honey solution is attached to the inner wall of the breeding container (FIG. 2 (j)). It is preferable to use the bait. For example, in a rearing container described later, a honey solution or the like may be applied to a part of the upper tube portion 1 or the mesh 8 (FIG. 2 (j)). Alternatively, absorbent cotton or the like soaked with honey liquid or the like may be placed on the mesh 8 (FIG. 2 (k)).

  When many next-generation adults of natural enemy insects appear (usually during the period of about 1 to 2 months after the introduction of natural enemies), a part is collected and reared in a new breeding container. By repeating the above procedure, the above natural enemy insects can be efficiently raised for generation without much effort.

  Natural enemy insects raised by the above method can be used for biological control of spider mites. In particular, it can be used effectively in fruit trees such as pears, mandarin oranges, apples, etc., for which biological control by phytoseiids is not sufficiently effective. Thereby, it can contribute to providing a safe and secure crop for consumers that does not depend on agricultural chemicals.

[2. Breeding container)
The breeding container according to the present invention is a breeding container used in the breeding method, and covers a cylindrical container having a bottom surface and an upper surface and an opening formed on the upper surface, and has an eye size of 0.01 mm or more. The cylindrical container is formed by detachably joining a cylindrical lower part having the bottom surface and a cylindrical upper part having the upper surface. The upper portion includes an engaging protrusion that abuts the inner surface of the lower portion in the seaming, and the upper portion is translucent.

  Hereinafter, with reference to FIG.1 and FIG.2 (i), an example of the breeding container based on this invention is demonstrated more concretely. A breeding container 10 shown in FIG. 1 is a cylindrical container having a circular bottom surface 4 and a circular top surface 3. The breeding container 10 has a cylindrical lower cylinder part (lower part) 2 having one end side opened and a bottom face 4 on the other end side, and a cylindrical upper cylinder part (upper part) having one end side opened and the upper face 3 on the other end side. 1 is a structure in which the one end sides are joined together. The joining of the upper cylinder part 1 and the lower cylinder part 2 is mechanical, and can be removed and reattached if necessary. For convenience of explanation, FIG. 1 shows a state before joining the upper tube portion 1 and the lower tube portion 2 or after removing both. FIG. 2I shows a state where the upper tube portion 1 and the lower tube portion 2 are joined together.

  On the upper surface 3, an opening (opening) 3a having a predetermined size is formed for ventilation. The opening 3 a is covered with a mesh 8, and when the upper tube portion 1 and the lower tube portion 2 are joined together, the interior space of the breeding container 10 and the outside communicate with each other only at the mesh 8 portion. The mesh size of the mesh 8 is in the range of 0.01 mm to 0.1 mm. As a result, extremely fine natural enemy insects (adult adults with a body length of 1 to 3 mm) and spider mites (adults with a body length of about 0.5 mm) can be isolated and bred while sufficiently fulfilling the function of ventilation. The material of the mesh 8 is not particularly limited, and a plankton net made by Tetorongose can be used.

  An opening 7 is formed in the side surface 9 b of the lower cylinder portion 2. Thereby, if necessary, the spider mites and natural enemy insects are introduced (for example, placed on a brush or the like on the plant for raising natural enemy insects) without opening and closing the upper cylinder part 1 and the lower cylinder part 2 or It can be collected (for example, sucked using a suction device). The position, number, size, shape, and the like of the opening 7 may be appropriately determined depending on the natural enemy insect to be bred or the type of plant for breeding the natural enemy insect. The opening 7 may be on the side surface 9a of the upper tube portion 1 and is not necessarily provided. The size of the opening 7 is preferably 5 mm to 30 mm in diameter. In order to prevent natural enemy insects and spider mites from escaping out of the breeding container 10, the opening 7 is closed with a stopper or the like, and the stopper or the like is removed only when necessary.

  Further, as shown in FIG. 1B, a step portion 5 is provided on the one end side of the lower cylinder portion 2 so that the radius of the cylinder constituting the inner surface of the lower cylinder portion 2 is enlarged. ing. On the other hand, a step portion (engagement protrusion) 6 is provided on the one end side of the upper tube portion 1 so that the radius of the cylinder constituting the outer surface of the upper tube portion 1 is reduced. When the lower tube portion 2 and the upper tube portion 1 are joined together, the inner surface (lower inner surface) 5a of the step portion 5 and the outer surface of the step portion 6 come into contact with each other, and the step portion 5 and the step portion 6 Are fitted together to form a seamless smooth surface. By providing this fitting structure, natural enemy insects and spider mites, which are micro organisms having a body length of about 0.5 mm to 3 mm, do not escape from the seam between the upper tube portion 1 and the lower tube portion 2. Yes.

  Moreover, at least the upper cylinder part 1 is comprised from the translucent material, Preferably both the upper cylinder part 1 and the lower cylinder part 2 are comprised from the translucent material. Thereby, the external light (for example, part or all of visible light) of a wavelength required for the photosynthesis of a plant can be taken in the breeding container 10. Examples of the translucent material include translucent resins such as acrylic, translucent ceramics, and glass. Among them, transparent materials such as acrylic resin and glass are preferable because the inside of the breeding container can be observed. Acrylic resins are more preferred because they are lightweight and easy to handle.

  In the case where the natural enemy and / or the potted plant for raising natural enemy insects is stored in the breeding container 10, a part of the upper cylinder part 1 and a part of the lower cylinder part 2 are kept in contact with each other. The upper tube portion 1 is tilted upward with the fitting structure of the step portion 5 and the step portion 6 as a fulcrum. At this time, since the movement of the outer surface of the step portion 6 of the upper tube portion 1 is restricted by the inner surface of the step portion 5, the upper tube portion 1 and the lower tube portion 2 can be opened and closed without being displaced. . The same applies to the case where the natural enemy and / or the pot-planted plant for raising natural enemy insects is taken out from the breeding container 10.

  The shape of the breeding container 10 is not particularly limited as long as it is cylindrical, and may be, for example, a rectangular column shape, an elliptical column shape, or the like. The height of the breeding container 10 may be appropriately determined depending on the type of pot-planted natural enemy insect breeding plant, and is, for example, in the range of 30 cm to 100 cm, and preferably in the range of 30 cm to 50 cm. The ratio of the height of the upper tube portion 1 and the lower tube portion 2 is not particularly limited. However, if the height of the lower tube portion 2 is within a range of 15 cm to 25 cm, the pot-planted natural enemy insect breeding plant is used. It is easy to put in and out, which is preferable. In addition, from the viewpoint of opening and closing the upper tube portion 1 and the lower tube portion 2 without shifting, the step portion 5 and the step portion 6 do not need to be formed in a continuous ring shape, but the natural enemy insects and spider mites From the standpoint of isolated breeding, the step portion 5 and the step portion 6 are preferably formed in a continuous ring shape.

  EXAMPLES Hereinafter, although an Example and a comparative example demonstrate this invention further in detail, this invention is not limited to this example.

  First, materials and methods commonly used in Examples 1 to 8 will be described together.

<Cultivation of spider mite breeding plants and mass propagation of spider mites>
Four black pots (9 cm in diameter) containing horticultural soil (kureha horticultural soil) were seeded with 3-5 grains of kidney bean seeds (variety: Nagazu peas) per pot, and bats (30 × 22 × 4. 5cm) and cultivated in a constant temperature room (23 ± 2 ° C., 1000-3500 lux, 16L8D). The next generation by placing several spider mite parasitic kidney bean leaves on the kidney bean plant about 2 to 3 weeks after sowing and keeping them isolated in a separate temperature-controlled room (23 ± 2 ° C., 1000 to 3500 lux, 16L8D) for 2 to 3 weeks. An individual was obtained (FIG. 2 (a)). This procedure was repeated to efficiently breed large numbers of spider mites (FIG. 2 (b)).

<Culture of plants for raising natural enemy insects>
In black pot (diameter 9cm) with horticulture soil (Kureha Horticulture soil), Komatsuna (variety: Rakuten, 1-4 per pot), Chingensai (variety: Blue Emperor Chingensai, Blue Axis Pakchoi, 1-3 per pot) Grain), Japanese radish (variety: Kanpaku, 1-3 per pot), Chinese cabbage (variety: disease resistant 60 days, 1-3 per pot), spinach (variety: Solomon, 1-2 per pot), or lettuce (Cultivar: Sunny lettuce, 1-2 seeds per pot) seeded, placed in a vat with water, and cultivated in a constant temperature room (23 ± 2 ° C, 8000-10000 lux, 16L8D) for about 2 weeks The plants were further cultivated in a greenhouse (23 ± 3 ° C.) for about 3 to 4 weeks.

<Infestation of spider mites on plants for raising natural enemy insects>
Place the pot-planted Komatsuna (5-6 weeks after sowing) obtained by the method of <Cultivation of Natural Enemy Insect-raising Plant> in a bat filled with water, and place several leafworm spider leaf beans on the Komatsuna plant body. After maintaining for 1 to 3 days in a temperature-controlled room (25 ± 1 ° C., 2000-5000 lux, 16L8D), by removing dead kidney bean leaves, a large number of nymph mites (100-400 adult females per pot, all A pot-planted komatsuna (hereinafter referred to as “spider mite parasitic komatsuna”) in a state in which 5 to 10 times of the growth stage was infested was obtained (FIG. 2 (c)). The spider mite parasitic kidney bean leaf was obtained according to the description in <Cultivation of spider mite propagation plant and spider mite proliferation>. And 1-2 pots of spider mite parasitic komatsuna were installed in the natural enemy breeding container mentioned later. Chinggensai, Japanese radish, Chinese cabbage, spinach and lettuce were also subjected to the same procedure as Komatsuna.

<Creation of natural enemy insects>
The natural enemy insects (Kiacyclohimetentoe (Fig. 2 (d)), red-eared thrips (Fig. 2 (e)), Japanese red spider mite, red spider mite, red spider fly (Fig. 2 (j)) It was introduced and raised in a natural enemy breeding container in which a parasitic komatsuna was installed, and the breeding container 10 shown in Fig. 1 was used as the natural enemy breeding container, which is made of acrylic resin and has an outer size of the lower tube portion 2. The height is about 180 mm, the outer dimension height of the upper tube portion 1 is about 210 mm, the top surface 3 and the bottom surface 4 are each a disc shape having a diameter of about 300 mm, and the opening 3a is a square hole of about 200 mm × 150 mm. Tetrangoth plankton net (Toray Star Night # 6000, eye size 0.04 mm) was used as the mesh 8. The top surface 3, the bottom surface 4 and the side surface 9a of the breeding container 10 were used. The thickness of 9b is about 5 mm, the height of the stepped portions 5 and 6 is about 5 mm, and the thickness is about 2.5 mm, and the opening 7 is 20 mm below the upper side of the lower tube portion 2. A circular hole with a diameter of 8.2 mm.

  After introduction of natural enemy insects, 1-2 pots of spider mite parasitic komatsuna were added every 7-10 days according to the amount of food in the natural enemy breeding container, and water was supplied to komatsuna every few days (FIG. 2 (f)). The old pots were appropriately removed according to the number of added pots (that is, the old and new pots were replaced). For poppy seeds, adults (Fig. 2 (g)), eggs and larvae live on the komatsuna pot, whereas pupae (Fig. 2 (h)) need to form and develop pupae in the soil. For this reason, moist vermiculite was laid on the bottom of the natural enemy breeding container for about 5 cm, and spider mite parasitic komatsuna was installed (FIG. 2 (i)). In spite of spider mites, larvae are predatory on spider mites, whereas adults eat nectar etc., so do absorbent cotton soaked with 50% honey solution on mesh 8 (Fig. 2 (k))? A honey solution or the like was applied to the inner wall surface or the like of the upper tube portion 1 of the mesh 8 or the breeding container 10 (FIG. 2 (j)) to obtain adult bait. Addition / exchange of spider mite parasites, introduction / collection of natural enemy insects, etc. were performed with the upper container of the natural enemy breeding container tilted. Since many next-generation adults of natural enemy insects appeared during the period of about 2 weeks to 1 month after introduction of natural enemy insects, some were collected and propagated in new natural enemy breeding containers. The above procedure was repeated to raise natural enemy insects. Chinggensai, Japanese radish, Chinese cabbage, spinach and lettuce were also subjected to the same procedure as Komatsuna.

  More specific methods and conditions will be individually described in the following examples.

<Example 1: Successive breeding of ladybird using spider mite parasitic komatsuna>
A natural enemy rearing vessel that collects about 20 adults and larvae that live on the waste leaves in the field and installs spider mite parasitic komatsuna obtained by the method of <Infestation of spider mites on natural enemy insect rearing plants> Was used for 4 months in a constant temperature room (25 ± 1 ° C., 16L8D). The method of breeding was in accordance with the method of <breding natural enemy insects> above. Under these conditions, the next generation adults of Chiacyclohime ladybirds could be obtained about every month, so every ladybird in the container was collected every month and introduced into a new natural enemy breeding container (container Maximum number of adults per 200).

  FIG. 3 shows the growth pattern of Kiacyclohimetento at that time. After 4 months (4 generation breeding), about 1600 adult Amaranthus japonicum, which is 80 times the introduction time, could be obtained. Including other developmental stages (eggs, larvae, and pupae) that are not the subject of this study suggests that a greater number of yellow-spotted beetles could be reared.

<Example 2: Growth rate of ladybird using spider mite parasitic komatsuna>
In this study, the growth rate was evaluated using as an index the number of next-generation adults per breeding container that had spawned adult Amaranthus japonica for a certain period of time. Introduce adult adults (200, almost same number of males and females) into a natural enemy breeding container in which spider mite parasitic komatsuna obtained by the above method <infestation of spider mites on natural enemy insect breeding plants> is installed. After the eggs were laid for a day, all the introduced adults were removed, and then the breeding test was started according to the method described above for breeding natural enemy insects. After the start of the test, spider larvae were appropriately fed with spider mite parasitic komatsuna and reared, and the number of emergence adults was investigated (repeated once). Other rearing conditions and methods are the same as in Example 1.

  The result is shown in FIG. A total of 420 next-generation adults could be obtained during the period of 15 to 21 days after the start of the test, suggesting that the growth rate of the natural enemy insects by spider parasite Komatsuna is high.

<Example 3: Rearing efficiency of ladybird when using spider mite parasitic komatsuna>
In this study, the breeding efficiency of ladybirds was evaluated using the rate at which larvae of the yellow-spotted beetle can grow to adults as an evaluation index. Spider mite parasitic Komatsuna obtained by the above method <Infestation of spider mites on plants for natural enemy insects> was inoculated with a small pen brush (1 to 2 years old, 400) and placed in a natural enemy breeding container. . Then, according to the above method of “raising natural enemy insects”, spider mite parasites were reared while appropriately exchanging spider mites, and the number of emerged adults was investigated (repeated twice). The spider mite parasitic Komatsuna is installed in a bat (11 cm in diameter) containing water in a natural enemy breeding container. Other rearing conditions and methods are the same as in Example 1.

  The result is shown in FIG. Although the test was repeated twice, in each case, 88.8% of the number of inoculated adults (355 animals each) was obtained during the period of 8 to 24 days after the start of the test. This result suggests that the spider mite parasite Komatsuna is suitable for the development of Larva and moths.

<Example 4: Rearing situation of ladybird using various pot planted plants>
Various spider mite parasitic plants (Komatsuna, Japanese radish, spinach, and lettuce) obtained by the above method <Infestation of spider mites on natural enemy insect rearing plants> were placed in a natural enemy rearing container. Next, 3 adult females of the common yellow-spotted beetle are introduced per natural enemy breeding container, and various spider mite parasitic plants infested with ticks are exchanged as appropriate according to the method of <bred of natural enemy insects> described above. Bred natural enemy insects. The number of next-generation natural enemies one month after the start of breeding of natural enemy insects was compared.

  Moreover, instead of the above-mentioned Komatsuna and Japanese radish, common bean and lentil as reference examples were reared according to this method. The kidney beans and lima bean (variety: Sieva) were cultivated for about 2 to 3 weeks according to the description in the section <Cultivation of spider mite propagation plant and spider mite proliferation>, and then the spider mite to the natural enemy insect rearing plant. The spider mite was infested in accordance with the description in the column of “Parasitic parasite>”.

  The results are shown in Table 1. In a test using Komatsuna, Japanese radish, spinach and lettuce (repeated once each), 35 to 94 next generation adults and about 110 other individuals were recovered after one month, and the number of adults was 12 at the time of introduction. It increased to about 30 times. These results suggest that potted cruciferous, red, and asteraceae vegetables can be easily grown by inoculating echidna mite.

  On the other hand, in the test using kidney beans and lima beans (4 repetitions each), the natural enemy insects were annihilated or hardly proliferated. This is because when the kidney beans and lima beans are inoculated with many spider mites, the plants die, and mold tends to grow in the natural enemy breeding container, and furthermore, natural enemy insects (especially larvae) on the key-shaped hair follicles that grow densely on the leaves of both. ) Was trapped and died. The above results suggest that the combination of spider mites and plants that have been used for breeding conventional natural enemy insects cannot sufficiently proliferate chiacyclohimetentou.

<Reference Example 1: Problems of the rearing method of Hadania thrips using conventional methods and materials>
In this study, problems in conventional breeding methods were examined. A kidney bean leaf piece (5 × 5 cm, leaf surface or leaf back) was placed on wet cotton wool (7 × 7 cm) in a round polystyrene container (diameter 9 cm, height 4.5 cm), After inoculation for 2 days, it was used for the test. Ten adult thrips were inoculated on the leaf mite leaf bean leaf, and the transition of the survival rate over 3 days was investigated (repeated 20 times each on the leaf front and back).

  The result is shown in FIG. The survival rate on the leaf surface of kidney bean leaves decreased to 79.3% 1 day after the start of the test, 70% after 2 days, and 52.7% after 3 days. As a cause of death, a case of death caused by hook-shaped hair follicles on the leaf pieces and a case of drowning on absorbent cotton were observed. The survival rate of the leaf of the kidney bean leaf was as low as 52.7% 1 day after the start of the test, and rapidly decreased to 34% after 2 days and 24% after 3 days.

  Regarding the scrap leaf pieces (diameter 8 cm, leaf surface or leaf back) (Fig. 7 (a)), 10 adults were inoculated and examined according to the same procedure and conditions (repeated 5 times each on the leaf surface and leaf back). ).

  The result is shown in FIG. The survival rate on the leaf surface of the waste leaf pieces was 30% 3 days after the start of the test, and the percentage of drowned individuals on absorbent cotton was 70%. The survival rate on the back side of the waste leaf pieces was 40% 3 days after the start of the test, and the percentage of drowned individuals was 60%. In either case, no mortality associated with stagnation was observed.

  The above results suggest that when the conventional materials and methods are used, the adult Thrips thrips die frequently, and the Thrips thrips cannot grow sufficiently, regardless of the type of leaf pieces and the front and back of the leaves. doing.

<Example 5: Breeding of Hanania thrips using pot-planted Komatsuna and other plants>
Spider mite parasite Komatsuna obtained by the above method <Infestation of spider mites on plants for breeding natural enemy insects> (Tick mite parasitized on plants 5 to 6 weeks after sowing, hereinafter "5 to 6 weeks after sowing") 1) was placed in a natural enemy breeding container, and then 5 or 20 adult female thrips were introduced (4 repetitions each). Then, according to the above method of <branching of natural enemy insects>, natural enemy insects were bred while appropriately adding spider mite parasitic komatsuna, and the number of next generations one month after introduction of natural enemy insects was investigated for each development stage. The procedure and conditions of each test were the same as in Example 1.

  Also, spider mite parasitic komatsuna with different elapsed days after sowing (3-4 weeks after sowing, or 7-8 weeks after sowing, infested with spider mites, hereinafter “3-4 weeks after sowing” or “ A test was also carried out under the same conditions except that "7 to 8 weeks after sowing" was used (in which 2 adults of Thrips thrips were introduced). In addition, a test was also conducted in which 20 adult females of Thrips thrips were introduced into the spider mite parasitic Komatsuna 7 to 8 weeks after sowing (repeated twice). Spider mite parasite Komatsuna 3 to 4 weeks after sowing or 7 to 8 weeks after sowing is in a constant temperature room (23 ± 2 ° C., 8000 to 10000 lux, 16L8D) in the method of <cultivation of natural enemy insect rearing plants>. After cultivating for about 2 weeks, in the greenhouse (23 ± 3 ° C.), each plant planted for about 1-2 weeks or 5-6 weeks was subjected to the above-mentioned <Infestation of spider mites on natural enemy insect breeding plants> The spider mite was infested by the method of>.

  Furthermore, a test in which four adult females of Thrips spider were introduced into the spider mite parasite radish (5-6 weeks after sowing) obtained by the above method <Infestation of spider mites on natural enemy insect breeding plants> A test in which 5 female adults were introduced into spider mite parasite (5-6 weeks after sowing) (3 repetitions), and a test in which 4 female adults were introduced into spider mite, 5-6 weeks after sowing (repetition was 1), 4 female adults were introduced into the spider mite spinach (5-6 weeks after sowing), and 4 female adults were introduced into the spider parasite lettuce (5-6 weeks after sowing). The test (repeated once) was performed in the same manner.

  Furthermore, as a comparison, a test (in which the repetition was performed once) in which 5 adult females of Thrips thrips were introduced into a spider mite parasite (2-3 weeks after sowing) obtained by the same method as in Example 4 was also performed.

  The results are shown in Table 2. In the test plot in which 5 natural enemy insects were introduced into the spider mite parasite Komatsuna (5-6 weeks after sowing), 169-229 next-generation female adults and about 200-500 other individuals were recovered one month later. The number of adults increased to about 34 to 46 times that at the time of introduction. In the test area where 20 natural enemy insects were introduced into the spider mite parasite Komatsuna (5-6 weeks after sowing), 394-564 next-generation female adults and about 100-2000 other individuals were recovered one month later. The number of adults increased to about 20 to 28 times that at the time of introduction.

  On the other hand, in the test plot where 5 natural enemy insects were introduced into the spider mite parasite Komatsuna (3-4 weeks after sowing), 70-88 next-generation female adults and about 140-370 other individuals were recovered one month later. The number of female adults increased to 14 to 18 times that at the time of introduction. In addition, in the test area where 5 natural enemy insects were introduced into the spider mite parasite Komatsuna (7-8 weeks after sowing), 64 next generation female adults and about 60-100 other individuals were recovered one month later. The number of adults increased to about 13 times that at the time of introduction. In the test plot in which 20 natural enemy insects were introduced into spider mite parasite Komatsuna (7-8 weeks after sowing), 122-172 next-generation female adults were collected one month later, and about 30-40 other individuals were recovered. The number of adults increased to about 6 to 9 times that at the time of introduction.

  The above results indicate that it is most preferable to inoculate the sand spider mite by inoculating the potted komatsuna 3 to 8 weeks after sowing, and further to inoculate the komatsuna 5 to 6 weeks after sowing. It suggests. The reason for this is presumed that Komatsuna 5 to 6 weeks after sowing is resistant to food damage stress even when inoculated with a large number of urticae, and the deterioration of old leaves is less likely to occur.

  In the test plot where 4 adult females of Thrips parasites were introduced into spider mite parasite, 174 next-generation female adults and about 140 other individuals were recovered after one month, and the number of adult females was approximately 43 times that of the introduction. Increased. In the test plot in which 5 female adults were introduced into the spider mite parasite, 91 to 224 next-generation female adults and about 70 to 380 other individuals were recovered one month later. It increased to about 45 times. In the test plot in which 4 female adults were introduced into the spider mite parasitic cabbage, 76 next-generation female adults and about 150 other individuals were recovered one month later, and the number of female adults increased 19-fold. In the test plot in which 4 female adults were introduced into the spider mite spinach, 67 next-generation female adults were recovered one month later, and the number of adult females increased 17-fold compared to the time of introduction. In the test plot where 4 female adults were introduced into the spider mite parasitic lettuce, 39 next-generation female adults were recovered after 1 month, and the number of adult females increased 10-fold compared to the time of introduction. The above results suggest that it is possible to easily proliferate the red sand thrips by inoculating potted cruciferous, red, and asteraceae vegetables.

  In a test in which 5 adult females of Thrips thrips were introduced into a pot plant spider mite parasite that was a comparison target, only 14 of the next generation female adults after 1 month were recovered and hardly proliferated. This suggests that the combination of spider mites and plants, which have been used for breeding natural enemy enemies in the past, does not allow the thrips to grow sufficiently.

<Example 6: Rearing of poppy seeds using spider mite parasitic komatsuna and other plants>
Using the spider mite parasite Komatsuna (5-6 weeks after sowing) obtained by the above method <Infestation of spider mites on plants for breeding natural enemy insects>, a breeding test was conducted on two types of poppy seed beetles. The experimental conditions and procedures were the same as in Example 1, but as described above, since the poppy wings grew in the soil, moist vermiculite was laid on the bottom of the natural enemy breeding container. Four adult females were introduced per natural enemy rearing container (repeated twice). In the same manner, 4 adult females of the spider mite, S. eleganae, which are closely related species, were introduced per natural enemy breeding container (repeated twice). Moreover, the spider mite parasitic spinach (5 to 6 weeks after sowing) and the spider mite parasitic lettuce (5 to 6 weeks after sowing) obtained by the same method as in Example 4 and the adult spider mite or spider mite beetle. Were introduced per natural enemy breeding container (repeated once each). Furthermore, as a comparison, four adult females of the spider mite, B. crispula, were introduced into the spider mite parasitic lima bean obtained by the same method as in Example 4 (one iteration).

  The results are shown in Table 3. In the test section where the spider mite was infected with the spider mite parasite Komatsuna, 162-167 next generation adults and 101 larvae were recovered one month after the introduction, and the number of adults increased by about 40 times the number of adults. did. In the test plot into which the adult spider mite, S. eleganae, was introduced, 109 to 141 next-generation adults were recovered one month after the introduction, and the number of adults increased approximately 27 to 35 times the number of adults. In addition, in the test section in which the spider mite parasitic spinach was introduced with the adult spider mite, the next generation adult, 67-104 adults were collected one month after the introduction, and the number of adults was 17-26 at the time of introduction. Increased about twice. In the test area where the spider mite parasite lettuce was introduced, the next generation adults were recovered one month after the introduction, and the number of adults increased to about 22 times the number of adults. These results suggest that poppy seeds can be easily propagated by inoculating potted cruciferous, red, and asteraceae vegetables with urticae.

  In the test area using the spider mite parasite Lima pea, which was the target of comparison, 70 adults were recovered one month after the introduction of the female spider mite, Brittany spp. Many were also observed. In addition, most adults did not settle on the plant body, but distributed on the inner wall of the breeding container, and inhabited spider mites that escaped from the plant body. The above results show that the combination of spider mites and plants that have been used for breeding conventional natural enemy insects cannot sufficiently develop larvae of poppy seeds, and adults do not sufficiently settle on plants, This suggests that poppy beetles cannot be propagated sufficiently.

<Example 7: Rearing of spider mite using spider mite parasitic komatsuna>
Spider mite parasite Komatsuna (5-6 weeks after sowing) obtained by the above method <Infestation of spider mite on plants for natural enemy insect rearing> is washed with natural enemy rearing container (as described above, absorbent cotton soaked with 50% honey solution After installing 1 pot in the mesh or applying honey solution to the inner wall of the upper part of the rearing container, etc., 15 spider mite larvae (4 repetitions) or 50 adults (2 repetitions) Introduced. Then, in accordance with the method of <branching of natural enemy insects>, natural enemy insects were bred while appropriately adding spider mite parasites, and the number of next-generation adults one month after the introduction of natural enemy insects was investigated. The procedure and conditions of each test were the same as in Example 1.

  The results are shown in Table 4. In the test plot in which 15 spider mite larvae were introduced into the spider mite parasite Komatsuna, 51 to 88 next-generation adults were recovered one month later, and the number of adults increased to about 3 to 6 times that at the time of introduction. In the test plot in which 50 spider mites were introduced into the spider mite parasite Komatsuna, the number of next-generation adults was recovered 224 to 245 after one month, and the number of adults increased to about 4 to 5 times the number at the time of introduction. Moreover, the test method of the honey liquid, which is an adult food, did not affect the growth rate of spider mites.

  The above results suggest that spider mites can be easily propagated by inoculating honey spider mites on a pot-planted Komatsuna 5 to 6 weeks after sowing and giving honey liquid as an adult food.

<Example 8: Rearing of spider mite using spider mite parasitic spinach>
After setting up 1 pot in a natural enemy breeding container prepared in the same manner as in Example 7, spider mite parasitic spinach (4-6 weeks after sowing) obtained by the method of <Infestation of spider mites on natural enemy insect breeding plants> 30 adult spider flies were introduced (repeated twice). Then, the number of next-generation adults two weeks after the introduction of natural enemy insects was investigated. The procedure and conditions of each test were the same as in Example 1.

  The results are shown in Table 5. In the test plot in which 30 spider mites were introduced into spider mite spinach (4-6 weeks after sowing), the number of next-generation adults was recovered after 2 weeks, and the number of adults was about three times that at the time of introduction. Increased. Moreover, the test method of the honey liquid, which is an adult food, did not affect the growth rate of spider mites.

  The above results suggest that spider mites can be easily proliferated by inoculating a potted spinach 4 to 6 weeks after sowing with a spider mite and giving a honey solution or the like as an adult diet.

  According to the present invention, natural enemies of spider mites can be reared efficiently and in large quantities.

1 ... Upper tube (upper part)
2 ... Lower cylinder part (lower part)
3... Upper surface 3a ..Opening (opening)
4 ・ ・ ・ Bottom surface 5a ・ ・ Inner side surface (lower inner surface)
6 ... Step part (engagement protrusion)
7 ・ ・ ・ Open 8 ・ ・ ・ Mesh 9a ・ ・ Side (side of upper cylinder)
9b ..Side (side of bottom cylinder)
10 · · Breeding containers (breeding containers and cylindrical containers)

Claims (12)

A growth process for growing spider mites;
A parasitic step of infesting the grown spider mites with at least one kind of vegetable selected from the group consisting of Brassicaceae, Asteraceae and Rubiaceae vegetables, and potted plants;
A method for raising natural enemy insects, comprising a step of placing the pot-planted vegetables infested with the mites in a breeding container and breeding the natural enemy insects of the spider mites in the breeding container.   The breeding method according to claim 1, wherein the vegetable is a cruciferous vegetable.   The breeding method according to claim 2, wherein the vegetable is Komatsuna.   In the proliferation step, the spider mites are propagated using at least one plant selected from the group consisting of leguminous, cruciferous, cucurbitaceae, eggplant, rose, and convolvulaceae plants. The breeding method according to any one of claims 1 to 3.   The breeding method according to claim 4, wherein the spider mites are propagated using a leguminous plant in the propagation step.   The breeding method according to claim 5, wherein in the breeding step, the spider mites are grown using kidney beans.   The breeding method according to any one of claims 4 to 6, wherein the plant is cultivated in an indoor environment in the propagation step.   The breeding method according to any one of claims 1 to 7, wherein the spider mite is a tick belonging to the genus Spider mite.   The breeding method according to claim 8, wherein the spider mite is a spider mite.   The breeding method according to any one of claims 1 to 9, wherein the natural enemy insect is at least one selected from the group consisting of a poppy, a ladybird, a thrips, and a fly fly. .   11. The breeding method according to claim 10, wherein the natural enemy insect is a spider mite, a spider mite, a spider mite, a thrips or a spider fly. The breeding container is
A cylindrical container having a bottom surface and a top surface;
It covers the opening formed on the upper surface, the size of the eye comprises a mesh in the range of 0.1mm or less 0.01mm or more,
The cylindrical container is
The cylindrical lower part having the bottom surface and the cylindrical upper part having the upper surface are detachably joined together,
The upper part includes an engaging protrusion that contacts the inner surface of the lower part in the seaming,
The upper is characterized by a translucent der Turkey, breeding method according to any one of claims 1 to 11.